AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0866290

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0866290 MtrunA17_Chr5g0422371 96.429 140 5 0 2 141 4 143 8.20e-93 271
Msa0866290 MtrunA17_Chr1g0203371 40.714 140 75 4 1 138 1 134 8.37e-24 94.0
Msa0866290 MtrunA17_Chr6g0456901 39.706 136 77 3 1 135 1 132 3.19e-23 92.8
Msa0866290 MtrunA17_Chr1g0161921 38.168 131 74 3 14 142 15 140 3.67e-22 89.4
Msa0866290 MtrunA17_Chr8g0375991 36.434 129 75 3 14 140 9 132 2.76e-17 76.6
Msa0866290 MtrunA17_Chr1g0161941 31.538 130 84 4 14 142 9 134 1.42e-14 69.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0866290 AT1G26100.1 59.859 142 46 1 1 142 1 131 6.09e-51 164
Msa0866290 AT5G38630.1 44.275 131 64 5 9 136 9 133 1.09e-26 102
Msa0866290 AT4G25570.2 39.552 134 76 3 9 141 49 178 1.20e-24 97.8
Msa0866290 AT4G25570.1 39.552 134 76 3 9 141 8 137 2.01e-24 96.3
Msa0866290 AT1G14730.1 30.556 144 90 4 14 152 20 158 7.22e-13 65.1

Find 88 sgRNAs with CRISPR-Local

Find 192 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTCAGTGGCTTTGTTATTT+TGG 0.056164 5_4:+67839414 Msa0866290:three_prime_UTR
GCTTTAGCTTCTGGGATATT+TGG 0.112597 5_4:+67837437 Msa0866290:CDS
TAGCATTTCTTGTTCTCTTC+TGG 0.118815 5_4:+67836997 Msa0866290:CDS
CAAGGGGTGGCTTTAGCTTC+TGG 0.175443 5_4:+67837428 Msa0866290:CDS
GAATGTTTGTGCAGCAATTT+TGG 0.224729 5_4:+67837349 Msa0866290:intron
TTACCTTGGCATGTATTTCT+TGG 0.229367 5_4:+67839250 Msa0866290:CDS
TTTGCTTTGTTGTAATTCTA+TGG 0.245295 5_4:+67836552 Msa0866290:five_prime_UTR
TTGATTTGTGTATCCCTCTT+TGG 0.260850 5_4:+67837530 Msa0866290:CDS
CTTTAGCTTCTGGGATATTT+GGG 0.267158 5_4:+67837438 Msa0866290:CDS
CTTCTGGGATATTTGGGATT+TGG 0.279862 5_4:+67837444 Msa0866290:CDS
TGTTATGACGCCATCTTAAT+TGG 0.285745 5_4:-67839588 None:intergenic
TCCTGTTGAGGAAGAGAATT+AGG 0.298620 5_4:-67837053 None:intergenic
ATTTCTACAGCCTTCATTCA+TGG 0.299138 5_4:+67837501 Msa0866290:CDS
CTCTTTCTTGCGAGAGTTTG+TGG 0.301862 5_4:+67836966 Msa0866290:CDS
GCAGTGGCTTCTTGAACTTT+TGG 0.301868 5_4:+67839191 Msa0866290:intron
AAGGGGTGGCTTTAGCTTCT+GGG 0.303519 5_4:+67837429 Msa0866290:CDS
GTTGTAAATTCTTTAGGCCT+TGG 0.309741 5_4:+67839382 Msa0866290:three_prime_UTR
AGCATTTCTTGTTCTCTTCT+GGG 0.319777 5_4:+67836998 Msa0866290:CDS
TACTACCTTCTCCACTTAAA+AGG 0.321243 5_4:-67837216 None:intergenic
CCACAATCCCATCATTTACT+TGG 0.326307 5_4:-67837475 None:intergenic
TCCATGGTTGTAAATTCTTT+AGG 0.339651 5_4:+67839376 Msa0866290:three_prime_UTR
CATCCTGTGCTCATGGTCAT+TGG 0.339718 5_4:+67837187 Msa0866290:CDS
ACATTTAGTTACAGTTGTTA+TGG 0.357021 5_4:+67836914 Msa0866290:exon
TCTTGTTCTCTTCTGGGCTC+TGG 0.357755 5_4:+67837004 Msa0866290:CDS
GCAGATCTTAGAATAGAAAA+TGG 0.360436 5_4:-67836641 None:intergenic
GCAGTAGCAACGGCGGAAAC+TGG 0.361598 5_4:+67839292 Msa0866290:CDS
TTGTTAGAATTTAGATATCT+TGG 0.364277 5_4:+67839558 Msa0866290:three_prime_UTR
TCCTAATTCTCTTCCTCAAC+AGG 0.404001 5_4:+67837052 Msa0866290:CDS
TGTATCCCTCTTTGGAGCTC+AGG 0.406685 5_4:+67837538 Msa0866290:CDS
TAGATGGTTGCCTGGGTCAA+GGG 0.416984 5_4:+67837376 Msa0866290:CDS
CTGCCAATGCATAAGTGTAT+AGG 0.426996 5_4:-67839273 None:intergenic
ACAAAATTCCAAGTAAATGA+TGG 0.437217 5_4:+67837467 Msa0866290:CDS
TTCTATTCTAAGATCTGCAA+TGG 0.437930 5_4:+67836646 Msa0866290:five_prime_UTR
TACAGCCTTCATTCATGGAT+GGG 0.445813 5_4:+67837506 Msa0866290:CDS
CATGGACTCAGTGCTGTGTT+TGG 0.448440 5_4:-67839359 None:intergenic
ATAGATGGTTGCCTGGGTCA+AGG 0.449560 5_4:+67837375 Msa0866290:CDS
GCGGCGACAACGGTCAGATC+TGG 0.449819 5_4:+67836735 Msa0866290:five_prime_UTR
GAAGAGAATTAGGAGTATTG+AGG 0.459271 5_4:-67837043 None:intergenic
AAATGCGATCAGTGAGGATA+AGG 0.461868 5_4:+67839224 Msa0866290:CDS
TCGGTGCATTTATGTCTTCA+AGG 0.466772 5_4:+67837410 Msa0866290:CDS
TTGGTGCATAGATGGTTGCC+TGG 0.471390 5_4:+67837368 Msa0866290:CDS
GTAAACAACGATTGTGTTGA+AGG 0.474451 5_4:+67836669 Msa0866290:five_prime_UTR
GGTTCTCCATCCTGTGCTCA+TGG 0.477323 5_4:+67837180 Msa0866290:intron
CTACAGCCTTCATTCATGGA+TGG 0.480089 5_4:+67837505 Msa0866290:CDS
AGATGGTTGCCTGGGTCAAG+GGG 0.483244 5_4:+67837377 Msa0866290:CDS
AAGAGCAAAGGGAGTAGTGA+GGG 0.491250 5_4:-67836948 None:intergenic
CTCTCGCAAGAAAGAGCAAA+GGG 0.500913 5_4:-67836959 None:intergenic
CGGTGCATTTATGTCTTCAA+GGG 0.507370 5_4:+67837411 Msa0866290:CDS
GTCAATTTGGTCGCAAACAA+AGG 0.515334 5_4:+67836586 Msa0866290:five_prime_UTR
TCTATTCTAAGATCTGCAAT+GGG 0.521294 5_4:+67836647 Msa0866290:five_prime_UTR
CAAACACAGCACTGAGTCCA+TGG 0.530906 5_4:+67839360 Msa0866290:exon
CAAAATTCCAAGTAAATGAT+GGG 0.535853 5_4:+67837468 Msa0866290:CDS
CCAAGTAAATGATGGGATTG+TGG 0.538655 5_4:+67837475 Msa0866290:CDS
TCAAACCCATCCATGAATGA+AGG 0.539092 5_4:-67837511 None:intergenic
GGATCTAAGAGCCAGGTTGT+CGG 0.540019 5_4:+67836822 Msa0866290:five_prime_UTR
GTGGATGGCTGCCGACAACC+TGG 0.544797 5_4:-67836833 None:intergenic
GCATTTATGTCTTCAAGGGG+TGG 0.549961 5_4:+67837415 Msa0866290:CDS
TGGCCTATACACTTATGCAT+TGG 0.552843 5_4:+67839270 Msa0866290:CDS
TGGTCACAAGCCAATTAAGA+TGG 0.558924 5_4:+67839578 Msa0866290:three_prime_UTR
TCACTGATCGCATTTCTCCT+CGG 0.562616 5_4:-67839216 None:intergenic
CTTGGTCTTGCTCTCCTCAG+TGG 0.566596 5_4:+67839400 Msa0866290:three_prime_UTR
AGGCCAAGAAATACATGCCA+AGG 0.572983 5_4:-67839253 None:intergenic
TGGTGCATAGATGGTTGCCT+GGG 0.586545 5_4:+67837369 Msa0866290:CDS
TTATGCATTGGCAGTAGCAA+CGG 0.591173 5_4:+67839282 Msa0866290:CDS
GCCTAAAGAATTTACAACCA+TGG 0.598613 5_4:-67839377 None:intergenic
AATCCAATGACCATGAGCAC+AGG 0.602677 5_4:-67837190 None:intergenic
GTTGCTAAACGCGGCGACAA+CGG 0.605493 5_4:+67836725 Msa0866290:five_prime_UTR
CTCACTACTAAATACACTCG+TGG 0.610031 5_4:-67836852 None:intergenic
CAATGACCATGAGCACAGGA+TGG 0.614301 5_4:-67837186 None:intergenic
CTACTAAATACACTCGTGGA+TGG 0.619730 5_4:-67836848 None:intergenic
TGAGGATAAGGATCTTACCT+TGG 0.620818 5_4:+67839236 Msa0866290:CDS
GATGGTTGCCTGGGTCAAGG+GGG 0.624454 5_4:+67837378 Msa0866290:CDS
AATGGTATCATACTGACAGA+AGG 0.626636 5_4:-67836623 None:intergenic
ACTCTCGCAAGAAAGAGCAA+AGG 0.634673 5_4:-67836960 None:intergenic
GAAGCAGTGGTTGCTAAACG+CGG 0.634869 5_4:+67836716 Msa0866290:five_prime_UTR
TACTCACCTGAGCTCCAAAG+AGG 0.638028 5_4:-67837544 None:intergenic
ACTCACCTGAGCTCCAAAGA+GGG 0.638153 5_4:-67837543 None:intergenic
TGCATTGGCAGTAGCAACGG+CGG 0.640059 5_4:+67839285 Msa0866290:CDS
CACTGAGGAGAGCAAGACCA+AGG 0.641031 5_4:-67839399 None:intergenic
GTGAGTGAGTGACTAGTGAG+AGG 0.642048 5_4:+67836870 Msa0866290:five_prime_UTR
CCAAAATAACAAAGCCACTG+AGG 0.646081 5_4:-67839414 None:intergenic
GGTGCATTTATGTCTTCAAG+GGG 0.646132 5_4:+67837412 Msa0866290:CDS
AAAGAGCAAAGGGAGTAGTG+AGG 0.647530 5_4:-67836949 None:intergenic
TAAACAACGATTGTGTTGAA+GGG 0.648525 5_4:+67836670 Msa0866290:five_prime_UTR
GCATAGATGAGATCCTGTTG+AGG 0.669004 5_4:-67837065 None:intergenic
GAGTAGTGAGGGAAAGACAG+TGG 0.669481 5_4:-67836937 None:intergenic
TAAATGTTGTGCAGAAGCAG+TGG 0.679941 5_4:+67836703 Msa0866290:five_prime_UTR
GAGGAGAAATGCGATCAGTG+AGG 0.738861 5_4:+67839218 Msa0866290:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTGATGTAATTAAATAAAAA+GGG - chr5_4:67837121-67837140 None:intergenic 10.0%
!! TTTGATGTAATTAAATAAAA+AGG - chr5_4:67837122-67837141 None:intergenic 10.0%
!!! TTTAATTTTAATTTAATTGC+AGG + chr5_4:67837159-67837178 Msa0866290:intron 10.0%
!!! TTCTAAATTAATTTTTACTT+AGG + chr5_4:67837605-67837624 Msa0866290:intron 10.0%
!! AATAATTTGATAAAAACTAA+AGG + chr5_4:67837778-67837797 Msa0866290:intron 10.0%
!! AATAATTTGATAAAAACTAA+AGG - chr5_4:67837778-67837797 None:intergenic 10.0%
!! GATAATAACAATTCATAATT+GGG - chr5_4:67838849-67838868 None:intergenic 15.0%
!! TGATAATAACAATTCATAAT+TGG - chr5_4:67838850-67838869 None:intergenic 15.0%
!! AACCCAAAAATATTATGTAT+TGG + chr5_4:67837252-67837271 Msa0866290:intron 20.0%
!! ACCCAAAAATATTATGTATT+GGG + chr5_4:67837253-67837272 Msa0866290:intron 20.0%
!!! TCCCAATACATAATATTTTT+GGG - chr5_4:67837257-67837276 None:intergenic 20.0%
!!! ATCCCAATACATAATATTTT+TGG - chr5_4:67837258-67837277 None:intergenic 20.0%
!! AAATATTATGTATTGGGATT+AGG + chr5_4:67837259-67837278 Msa0866290:intron 20.0%
!! TAATGAAATCAAGTCATTAT+TGG + chr5_4:67837292-67837311 Msa0866290:intron 20.0%
!! AATACATGTATGTTAATTGA+TGG + chr5_4:67837680-67837699 Msa0866290:intron 20.0%
!!! TATGTTAACTGATGTATTTT+TGG + chr5_4:67838483-67838502 Msa0866290:intron 20.0%
!! AGCAACTAAATTGAAATAAA+AGG - chr5_4:67838779-67838798 None:intergenic 20.0%
!! ACACAATTAATTTCTATCAA+TGG - chr5_4:67838924-67838943 None:intergenic 20.0%
!!! GCAAAATTCTTTTATGTATA+TGG + chr5_4:67839115-67839134 Msa0866290:intron 20.0%
!!! TTTTGAAGAATTTGATACTA+AGG - chr5_4:67839451-67839470 None:intergenic 20.0%
!! TTGTTAGAATTTAGATATCT+TGG + chr5_4:67839558-67839577 Msa0866290:three_prime_UTR 20.0%
! ACAACAAAGCAAAAAACTAA+AGG - chr5_4:67836545-67836564 None:intergenic 25.0%
!! TTTGCTTTGTTGTAATTCTA+TGG + chr5_4:67836552-67836571 Msa0866290:five_prime_UTR 25.0%
! ACATTTAGTTACAGTTGTTA+TGG + chr5_4:67836914-67836933 Msa0866290:exon 25.0%
!! ATTGGATTTATCCTTTTAAG+TGG + chr5_4:67837205-67837224 Msa0866290:CDS 25.0%
! ACAAAATTCCAAGTAAATGA+TGG + chr5_4:67837467-67837486 Msa0866290:CDS 25.0%
! CAAAATTCCAAGTAAATGAT+GGG + chr5_4:67837468-67837487 Msa0866290:CDS 25.0%
!!! TTAATTGATGGTGATTTTGT+TGG + chr5_4:67837692-67837711 Msa0866290:intron 25.0%
!! TTTTATGGTGAACTTTACAA+TGG - chr5_4:67837853-67837872 None:intergenic 25.0%
!!! TGAGAGTGTATTGTTTTTTA+TGG - chr5_4:67838043-67838062 None:intergenic 25.0%
! AATGCATATATGTTAAGTGA+TGG - chr5_4:67838231-67838250 None:intergenic 25.0%
! TTAATTGATTGCGATATTGT+CGG - chr5_4:67838256-67838275 None:intergenic 25.0%
!! TATGTTGATTGATTGTGATA+TGG - chr5_4:67838333-67838352 None:intergenic 25.0%
! TTGATTGATTGTGATATTGT+TGG - chr5_4:67838362-67838381 None:intergenic 25.0%
! TATTTCTCACAATTTAAGTG+AGG + chr5_4:67838429-67838448 Msa0866290:intron 25.0%
! ATTTCTCACAATTTAAGTGA+GGG + chr5_4:67838430-67838449 Msa0866290:intron 25.0%
! AACAATAAGAAACTGGAATT+TGG - chr5_4:67838754-67838773 None:intergenic 25.0%
! AAAGGATAACAATAAGAAAC+TGG - chr5_4:67838761-67838780 None:intergenic 25.0%
! GGCTTTTACTTATGTCAATT+TGG + chr5_4:67836573-67836592 Msa0866290:five_prime_UTR 30.0%
GCAGATCTTAGAATAGAAAA+TGG - chr5_4:67836644-67836663 None:intergenic 30.0%
!! TTCTATTCTAAGATCTGCAA+TGG + chr5_4:67836646-67836665 Msa0866290:five_prime_UTR 30.0%
!! TCTATTCTAAGATCTGCAAT+GGG + chr5_4:67836647-67836666 Msa0866290:five_prime_UTR 30.0%
TAAACAACGATTGTGTTGAA+GGG + chr5_4:67836670-67836689 Msa0866290:five_prime_UTR 30.0%
AGAAAAAGAAAGAGAGAAAG+AGG - chr5_4:67836777-67836796 None:intergenic 30.0%
!!! ATCCACTTTTTCTTCCATAA+CGG - chr5_4:67836806-67836825 None:intergenic 30.0%
AAAGGGAATGAAGAAAATAG+AGG - chr5_4:67837104-67837123 None:intergenic 30.0%
!! TTTATCCTTTTAAGTGGAGA+AGG + chr5_4:67837211-67837230 Msa0866290:CDS 30.0%
ATGACTTAAATTGGAGATAG+AGG - chr5_4:67838003-67838022 None:intergenic 30.0%
TCTATCTCCAATTTAAGTCA+TGG + chr5_4:67837907-67837926 Msa0866290:intron 30.0%
! ACTTTTGCCATGACTTAAAT+TGG - chr5_4:67837994-67838013 None:intergenic 30.0%
ATTTAAGTCATGGCAAAAGT+AGG + chr5_4:67837917-67837936 Msa0866290:intron 30.0%
ATTTAAGTCATGGCAAAAGT+AGG - chr5_4:67837917-67837936 None:intergenic 30.0%
! ACTTTTGCCATGACTTAAAT+TGG + chr5_4:67837994-67838013 Msa0866290:intron 30.0%
TCTATCTCCAATTTAAGTCA+TGG - chr5_4:67837907-67837926 None:intergenic 30.0%
ATGACTTAAATTGGAGATAG+AGG + chr5_4:67838003-67838022 Msa0866290:intron 30.0%
!! TTAAGTGATGGAGATTTTGT+TGG - chr5_4:67838219-67838238 None:intergenic 30.0%
TTGATTGATTGCGATATTGT+CGG - chr5_4:67838292-67838311 None:intergenic 30.0%
TTGATTGATTGTGATATGGT+TGG - chr5_4:67838329-67838348 None:intergenic 30.0%
TTTCTCACAATTTAAGTGAG+GGG + chr5_4:67838431-67838450 Msa0866290:intron 30.0%
TACTTACTGTATACATAGTG+TGG - chr5_4:67838465-67838484 None:intergenic 30.0%
TCAGGACATACATGAATAAA+GGG - chr5_4:67838804-67838823 None:intergenic 30.0%
ATCAGGACATACATGAATAA+AGG - chr5_4:67838805-67838824 None:intergenic 30.0%
! TGAATTGTTATTATCACCAC+TGG + chr5_4:67838854-67838873 Msa0866290:intron 30.0%
TATATGCACAGCAATAACAA+GGG - chr5_4:67838956-67838975 None:intergenic 30.0%
TTATATGCACAGCAATAACA+AGG - chr5_4:67838957-67838976 None:intergenic 30.0%
TCCATGGTTGTAAATTCTTT+AGG + chr5_4:67839376-67839395 Msa0866290:three_prime_UTR 30.0%
! AACTTTTGTACTCAGATACT+AGG + chr5_4:67839473-67839492 Msa0866290:three_prime_UTR 30.0%
AATGGTATCATACTGACAGA+AGG - chr5_4:67836626-67836645 None:intergenic 35.0%
GTAAACAACGATTGTGTTGA+AGG + chr5_4:67836669-67836688 Msa0866290:five_prime_UTR 35.0%
!! CTTTTTCTGAAAACCCGTTA+TGG + chr5_4:67836789-67836808 Msa0866290:five_prime_UTR 35.0%
!!! TCCACTTTTTCTTCCATAAC+GGG - chr5_4:67836805-67836824 None:intergenic 35.0%
TAGCATTTCTTGTTCTCTTC+TGG + chr5_4:67836997-67837016 Msa0866290:CDS 35.0%
AGCATTTCTTGTTCTCTTCT+GGG + chr5_4:67836998-67837017 Msa0866290:CDS 35.0%
!! GAAGAGAATTAGGAGTATTG+AGG - chr5_4:67837046-67837065 None:intergenic 35.0%
TACTACCTTCTCCACTTAAA+AGG - chr5_4:67837219-67837238 None:intergenic 35.0%
! GAATGTTTGTGCAGCAATTT+TGG + chr5_4:67837349-67837368 Msa0866290:intron 35.0%
!! CTTTAGCTTCTGGGATATTT+GGG + chr5_4:67837438-67837457 Msa0866290:CDS 35.0%
ATTTCTACAGCCTTCATTCA+TGG + chr5_4:67837501-67837520 Msa0866290:CDS 35.0%
!! TTGATTTGTGTATCCCTCTT+TGG + chr5_4:67837530-67837549 Msa0866290:CDS 35.0%
TGGAGATAGAGGAAAAAAGA+AGG - chr5_4:67838014-67838033 None:intergenic 35.0%
GCAAAAGTAGGAATTAGTGT+TGG + chr5_4:67837929-67837948 Msa0866290:intron 35.0%
!! AGTGTTGGTCTGAAGTAATA+CGG - chr5_4:67837967-67837986 None:intergenic 35.0%
TGGAGATAGAGGAAAAAAGA+AGG + chr5_4:67838014-67838033 Msa0866290:intron 35.0%
!!! GTATTTTGGTCAGTTTGACT+GGG - chr5_4:67838606-67838625 None:intergenic 35.0%
! TAACTAACGCATGGGTATTT+TGG - chr5_4:67838620-67838639 None:intergenic 35.0%
AATCGCTACGTGAATTCAAA+TGG - chr5_4:67838724-67838743 None:intergenic 35.0%
CAGGACATACATGAATAAAG+GGG - chr5_4:67838803-67838822 None:intergenic 35.0%
ATATGCACAGCAATAACAAG+GGG - chr5_4:67838955-67838974 None:intergenic 35.0%
GGAAATTGTAAACTAGTGTG+AGG + chr5_4:67838997-67839016 Msa0866290:intron 35.0%
CAACATGTTGAACTTCGTTA+TGG - chr5_4:67839167-67839186 None:intergenic 35.0%
TTACCTTGGCATGTATTTCT+TGG + chr5_4:67839250-67839269 Msa0866290:CDS 35.0%
GCCTAAAGAATTTACAACCA+TGG - chr5_4:67839380-67839399 None:intergenic 35.0%
GTTGTAAATTCTTTAGGCCT+TGG + chr5_4:67839382-67839401 Msa0866290:three_prime_UTR 35.0%
TGTTATGACGCCATCTTAAT+TGG - chr5_4:67839591-67839610 None:intergenic 35.0%
GTCAATTTGGTCGCAAACAA+AGG + chr5_4:67836586-67836605 Msa0866290:five_prime_UTR 40.0%
TAAATGTTGTGCAGAAGCAG+TGG + chr5_4:67836703-67836722 Msa0866290:five_prime_UTR 40.0%
ACCCGTTATGGAAGAAAAAG+TGG + chr5_4:67836801-67836820 Msa0866290:five_prime_UTR 40.0%
AAAAAGTGGATCTAAGAGCC+AGG + chr5_4:67836815-67836834 Msa0866290:five_prime_UTR 40.0%
CTACTAAATACACTCGTGGA+TGG - chr5_4:67836851-67836870 None:intergenic 40.0%
CTCACTACTAAATACACTCG+TGG - chr5_4:67836855-67836874 None:intergenic 40.0%
TCCTAATTCTCTTCCTCAAC+AGG + chr5_4:67837052-67837071 Msa0866290:CDS 40.0%
TCCTGTTGAGGAAGAGAATT+AGG - chr5_4:67837056-67837075 None:intergenic 40.0%
!! CAGCAATTTTGGTGCATAGA+TGG + chr5_4:67837360-67837379 Msa0866290:intron 40.0%
TCGGTGCATTTATGTCTTCA+AGG + chr5_4:67837410-67837429 Msa0866290:CDS 40.0%
CGGTGCATTTATGTCTTCAA+GGG + chr5_4:67837411-67837430 Msa0866290:CDS 40.0%
GGTGCATTTATGTCTTCAAG+GGG + chr5_4:67837412-67837431 Msa0866290:CDS 40.0%
!! GCTTTAGCTTCTGGGATATT+TGG + chr5_4:67837437-67837456 Msa0866290:CDS 40.0%
!! CTTCTGGGATATTTGGGATT+TGG + chr5_4:67837444-67837463 Msa0866290:CDS 40.0%
CCACAATCCCATCATTTACT+TGG - chr5_4:67837478-67837497 None:intergenic 40.0%
! CCAAGTAAATGATGGGATTG+TGG + chr5_4:67837475-67837494 Msa0866290:CDS 40.0%
TACAGCCTTCATTCATGGAT+GGG + chr5_4:67837506-67837525 Msa0866290:CDS 40.0%
TCAAACCCATCCATGAATGA+AGG - chr5_4:67837514-67837533 None:intergenic 40.0%
GCAAAAGTAGGAGTTAGTGT+TGG - chr5_4:67837982-67838001 None:intergenic 40.0%
!!! GGTATTTTGGTCAGTTTGAC+TGG - chr5_4:67838607-67838626 None:intergenic 40.0%
GCGTTAGTTAACTAACGCAT+GGG - chr5_4:67838628-67838647 None:intergenic 40.0%
TTGCGTGAGTTAACTTACAC+TGG + chr5_4:67838679-67838698 Msa0866290:intron 40.0%
! AAGTGAAAAGCGCAACAATC+AGG - chr5_4:67838822-67838841 None:intergenic 40.0%
TCAAAAAGTCAAGCCTTGCA+CGG - chr5_4:67838892-67838911 None:intergenic 40.0%
! AACTGCTGTAGCTGTTCTTT+TGG + chr5_4:67838976-67838995 Msa0866290:intron 40.0%
TGCGTTAGTTAACTAACGCA+AGG + chr5_4:67839043-67839062 Msa0866290:intron 40.0%
GCGTTAGTTAACTAACGCAA+GGG + chr5_4:67839044-67839063 Msa0866290:intron 40.0%
!!! AACTAACGCAAGGGCATTTT+TGG + chr5_4:67839053-67839072 Msa0866290:intron 40.0%
!!! GCATTTTTGGCATGGCTAAA+AGG + chr5_4:67839066-67839085 Msa0866290:intron 40.0%
TTCAACATGTTGCATTGCAG+TGG + chr5_4:67839175-67839194 Msa0866290:intron 40.0%
AAATGCGATCAGTGAGGATA+AGG + chr5_4:67839224-67839243 Msa0866290:CDS 40.0%
TGAGGATAAGGATCTTACCT+TGG + chr5_4:67839236-67839255 Msa0866290:CDS 40.0%
TGGCCTATACACTTATGCAT+TGG + chr5_4:67839270-67839289 Msa0866290:CDS 40.0%
CTGCCAATGCATAAGTGTAT+AGG - chr5_4:67839276-67839295 None:intergenic 40.0%
! TTATGCATTGGCAGTAGCAA+CGG + chr5_4:67839282-67839301 Msa0866290:CDS 40.0%
CCAAAATAACAAAGCCACTG+AGG - chr5_4:67839417-67839436 None:intergenic 40.0%
!!! CCTCAGTGGCTTTGTTATTT+TGG + chr5_4:67839414-67839433 Msa0866290:three_prime_UTR 40.0%
TGGTCACAAGCCAATTAAGA+TGG + chr5_4:67839578-67839597 Msa0866290:three_prime_UTR 40.0%
AAGAGCAAAGGGAGTAGTGA+GGG - chr5_4:67836951-67836970 None:intergenic 45.0%
AAAGAGCAAAGGGAGTAGTG+AGG - chr5_4:67836952-67836971 None:intergenic 45.0%
CTCTCGCAAGAAAGAGCAAA+GGG - chr5_4:67836962-67836981 None:intergenic 45.0%
ACTCTCGCAAGAAAGAGCAA+AGG - chr5_4:67836963-67836982 None:intergenic 45.0%
CTCTTTCTTGCGAGAGTTTG+TGG + chr5_4:67836966-67836985 Msa0866290:CDS 45.0%
GCATAGATGAGATCCTGTTG+AGG - chr5_4:67837068-67837087 None:intergenic 45.0%
AATCCAATGACCATGAGCAC+AGG - chr5_4:67837193-67837212 None:intergenic 45.0%
GTCAAGGGGGTTGAAGAAAT+CGG + chr5_4:67837391-67837410 Msa0866290:CDS 45.0%
GCATTTATGTCTTCAAGGGG+TGG + chr5_4:67837415-67837434 Msa0866290:CDS 45.0%
CTACAGCCTTCATTCATGGA+TGG + chr5_4:67837505-67837524 Msa0866290:CDS 45.0%
TTTGGTCAGTTTGACTGGGT+GGG - chr5_4:67838602-67838621 None:intergenic 45.0%
! TTTTGGTCAGTTTGACTGGG+TGG - chr5_4:67838603-67838622 None:intergenic 45.0%
GGCGTTAGTTAACTAACGCA+TGG - chr5_4:67838629-67838648 None:intergenic 45.0%
ACGGTATATTACGACACCAG+TGG - chr5_4:67838873-67838892 None:intergenic 45.0%
GTGTCGTAATATACCGTGCA+AGG + chr5_4:67838876-67838895 Msa0866290:intron 45.0%
! GCAGTGGCTTCTTGAACTTT+TGG + chr5_4:67839191-67839210 Msa0866290:intron 45.0%
! TTCTTGAACTTTTGGCACCG+AGG + chr5_4:67839199-67839218 Msa0866290:CDS 45.0%
TCACTGATCGCATTTCTCCT+CGG - chr5_4:67839219-67839238 None:intergenic 45.0%
AGGCCAAGAAATACATGCCA+AGG - chr5_4:67839256-67839275 None:intergenic 45.0%
GAAGCAGTGGTTGCTAAACG+CGG + chr5_4:67836716-67836735 Msa0866290:five_prime_UTR 50.0%
GGATCTAAGAGCCAGGTTGT+CGG + chr5_4:67836822-67836841 Msa0866290:five_prime_UTR 50.0%
GTGAGTGAGTGACTAGTGAG+AGG + chr5_4:67836870-67836889 Msa0866290:five_prime_UTR 50.0%
GAGTAGTGAGGGAAAGACAG+TGG - chr5_4:67836940-67836959 None:intergenic 50.0%
TCTTGTTCTCTTCTGGGCTC+TGG + chr5_4:67837004-67837023 Msa0866290:CDS 50.0%
CAATGACCATGAGCACAGGA+TGG - chr5_4:67837189-67837208 None:intergenic 50.0%
CATCCTGTGCTCATGGTCAT+TGG + chr5_4:67837187-67837206 Msa0866290:CDS 50.0%
TTGGTGCATAGATGGTTGCC+TGG + chr5_4:67837368-67837387 Msa0866290:CDS 50.0%
TGGTGCATAGATGGTTGCCT+GGG + chr5_4:67837369-67837388 Msa0866290:CDS 50.0%
ATAGATGGTTGCCTGGGTCA+AGG + chr5_4:67837375-67837394 Msa0866290:CDS 50.0%
TAGATGGTTGCCTGGGTCAA+GGG + chr5_4:67837376-67837395 Msa0866290:CDS 50.0%
!! AAGGGGTGGCTTTAGCTTCT+GGG + chr5_4:67837429-67837448 Msa0866290:CDS 50.0%
!! TGTATCCCTCTTTGGAGCTC+AGG + chr5_4:67837538-67837557 Msa0866290:CDS 50.0%
ACTCACCTGAGCTCCAAAGA+GGG - chr5_4:67837546-67837565 None:intergenic 50.0%
TACTCACCTGAGCTCCAAAG+AGG - chr5_4:67837547-67837566 None:intergenic 50.0%
! CCAGCGTGAGTTAAGTTGCA+CGG - chr5_4:67838650-67838669 None:intergenic 50.0%
GCACAGCAATAACAAGGGGT+TGG - chr5_4:67838951-67838970 None:intergenic 50.0%
!!! ACGCAAGGGCATTTTTGGCA+TGG + chr5_4:67839058-67839077 Msa0866290:intron 50.0%
! AAGGCGCGAGTGAAAAGTGT+AGG + chr5_4:67839085-67839104 Msa0866290:intron 50.0%
! AGGCGCGAGTGAAAAGTGTA+GGG + chr5_4:67839086-67839105 Msa0866290:intron 50.0%
GAGGAGAAATGCGATCAGTG+AGG + chr5_4:67839218-67839237 Msa0866290:CDS 50.0%
CATGGACTCAGTGCTGTGTT+TGG - chr5_4:67839362-67839381 None:intergenic 50.0%
! CAAACACAGCACTGAGTCCA+TGG + chr5_4:67839360-67839379 Msa0866290:exon 50.0%
GTTGCTAAACGCGGCGACAA+CGG + chr5_4:67836725-67836744 Msa0866290:five_prime_UTR 55.0%
GGTTCTCCATCCTGTGCTCA+TGG + chr5_4:67837180-67837199 Msa0866290:intron 55.0%
AGATGGTTGCCTGGGTCAAG+GGG + chr5_4:67837377-67837396 Msa0866290:CDS 55.0%
TTCTTCAACCCCCTTGACCC+AGG - chr5_4:67837389-67837408 None:intergenic 55.0%
!! CAAGGGGTGGCTTTAGCTTC+TGG + chr5_4:67837428-67837447 Msa0866290:CDS 55.0%
! TGGGAGCGAAAAGTGCAGAG+GGG - chr5_4:67838583-67838602 None:intergenic 55.0%
! GTGGGAGCGAAAAGTGCAGA+GGG - chr5_4:67838584-67838603 None:intergenic 55.0%
CCGTGCAACTTAACTCACGC+TGG + chr5_4:67838647-67838666 Msa0866290:intron 55.0%
! GGCGCGAGTGAAAAGTGTAG+GGG + chr5_4:67839087-67839106 Msa0866290:intron 55.0%
! GCGCGAGTGAAAAGTGTAGG+GGG + chr5_4:67839088-67839107 Msa0866290:intron 55.0%
! TGCATTGGCAGTAGCAACGG+CGG + chr5_4:67839285-67839304 Msa0866290:CDS 55.0%
CACTGAGGAGAGCAAGACCA+AGG - chr5_4:67839402-67839421 None:intergenic 55.0%
!! CTTGGTCTTGCTCTCCTCAG+TGG + chr5_4:67839400-67839419 Msa0866290:three_prime_UTR 55.0%
GATGGTTGCCTGGGTCAAGG+GGG + chr5_4:67837378-67837397 Msa0866290:CDS 60.0%
! GGTGGGAGCGAAAAGTGCAG+AGG - chr5_4:67838585-67838604 None:intergenic 60.0%
GCAGTAGCAACGGCGGAAAC+TGG + chr5_4:67839292-67839311 Msa0866290:CDS 60.0%
GCGGCGACAACGGTCAGATC+TGG + chr5_4:67836735-67836754 Msa0866290:five_prime_UTR 65.0%
GTGGATGGCTGCCGACAACC+TGG - chr5_4:67836836-67836855 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 67836476 67839627 67836476 ID=Msa0866290;Name=Msa0866290
chr5_4 mRNA 67836476 67839627 67836476 ID=Msa0866290-mRNA-1;Parent=Msa0866290;Name=Msa0866290-mRNA-1;_AED=0.25;_eAED=0.25;_QI=457|1|1|1|1|1|4|253|194
chr5_4 exon 67836476 67837088 67836476 ID=Msa0866290-mRNA-1:exon:21435;Parent=Msa0866290-mRNA-1
chr5_4 exon 67837181 67837232 67837181 ID=Msa0866290-mRNA-1:exon:21436;Parent=Msa0866290-mRNA-1
chr5_4 exon 67837363 67837559 67837363 ID=Msa0866290-mRNA-1:exon:21437;Parent=Msa0866290-mRNA-1
chr5_4 exon 67839195 67839627 67839195 ID=Msa0866290-mRNA-1:exon:21438;Parent=Msa0866290-mRNA-1
chr5_4 five_prime_UTR 67836476 67836932 67836476 ID=Msa0866290-mRNA-1:five_prime_utr;Parent=Msa0866290-mRNA-1
chr5_4 CDS 67836933 67837088 67836933 ID=Msa0866290-mRNA-1:cds;Parent=Msa0866290-mRNA-1
chr5_4 CDS 67837181 67837232 67837181 ID=Msa0866290-mRNA-1:cds;Parent=Msa0866290-mRNA-1
chr5_4 CDS 67837363 67837559 67837363 ID=Msa0866290-mRNA-1:cds;Parent=Msa0866290-mRNA-1
chr5_4 CDS 67839195 67839374 67839195 ID=Msa0866290-mRNA-1:cds;Parent=Msa0866290-mRNA-1
chr5_4 three_prime_UTR 67839375 67839627 67839375 ID=Msa0866290-mRNA-1:three_prime_utr;Parent=Msa0866290-mRNA-1
Gene Sequence

>Msa0866290

ATGGCCACTGTCTTTCCCTCACTACTCCCTTTGCTCTTTCTTGCGAGAGTTTGTGGTCTTTCAGTAGCATTTCTTGTTCTCTTCTGGGCTCTGGCTTTCAAATCTAGCTTCCTCAATACTCCTAATTCTCTTCCTCAACAGGATCTCATCTATGCAGTTCTCCATCCTGTGCTCATGGTCATTGGATTTATCCTTTTAAGTGGAGAAGCAATTTTGGTGCATAGATGGTTGCCTGGGTCAAGGGGGTTGAAGAAATCGGTGCATTTATGTCTTCAAGGGGTGGCTTTAGCTTCTGGGATATTTGGGATTTGGACAAAATTCCAAGTAAATGATGGGATTGTGGCTAATTTCTACAGCCTTCATTCATGGATGGGTTTGATTTGTGTATCCCTCTTTGGAGCTCAGTGGCTTCTTGAACTTTTGGCACCGAGGAGAAATGCGATCAGTGAGGATAAGGATCTTACCTTGGCATGTATTTCTTGGCCTATACACTTATGCATTGGCAGTAGCAACGGCGGAAACTGGATTATTAGAGAAGCTGACATTCTTGCAAACACAGAGAAATGTATCCAAACACAGCACTGA
Protein sequence

>Msa0866290

MATVFPSLLPLLFLARVCGLSVAFLVLFWALAFKSSFLNTPNSLPQQDLIYAVLHPVLMVIGFILLSGEAILVHRWLPGSRGLKKSVHLCLQGVALASGIFGIWTKFQVNDGIVANFYSLHSWMGLICVSLFGAQWLLELLAPRRNAISEDKDLTLACISWPIHLCIGSSNGGNWIIREADILANTEKCIQTQH*