AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0729100

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0729100 MtrunA17_Chr5g0422371 96.429 140 5 0 2 141 4 143 8.20e-93 271
Msa0729100 MtrunA17_Chr1g0203371 40.714 140 75 4 1 138 1 134 8.37e-24 94.0
Msa0729100 MtrunA17_Chr6g0456901 39.706 136 77 3 1 135 1 132 3.19e-23 92.8
Msa0729100 MtrunA17_Chr1g0161921 38.168 131 74 3 14 142 15 140 3.67e-22 89.4
Msa0729100 MtrunA17_Chr8g0375991 36.434 129 75 3 14 140 9 132 2.76e-17 76.6
Msa0729100 MtrunA17_Chr1g0161941 31.538 130 84 4 14 142 9 134 1.42e-14 69.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0729100 AT1G26100.1 59.859 142 46 1 1 142 1 131 6.09e-51 164
Msa0729100 AT5G38630.1 44.275 131 64 5 9 136 9 133 1.09e-26 102
Msa0729100 AT4G25570.2 39.552 134 76 3 9 141 49 178 1.20e-24 97.8
Msa0729100 AT4G25570.1 39.552 134 76 3 9 141 8 137 2.01e-24 96.3
Msa0729100 AT1G14730.1 30.556 144 90 4 14 152 20 158 7.22e-13 65.1

Find 92 sgRNAs with CRISPR-Local

Find 191 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTCAGTGGCTTTGTTATTT+TGG 0.056164 5_1:+35572500 Msa0729100:three_prime_UTR
GCTTTAGCTTCTGGGATATT+TGG 0.112597 5_1:+35570533 Msa0729100:CDS
TAGCATTTCTTGTTCTCTTC+TGG 0.118815 5_1:+35570093 Msa0729100:CDS
CAAGGGGTGGCTTTAGCTTC+TGG 0.175443 5_1:+35570524 Msa0729100:CDS
GAATGTTTGTGCAGCAATTT+TGG 0.224729 5_1:+35570445 Msa0729100:intron
TTACCTTGGCATGTATTTCT+TGG 0.229367 5_1:+35572336 Msa0729100:CDS
TTTGCTTTGTTGTAATTCTA+TGG 0.245295 5_1:+35569648 Msa0729100:five_prime_UTR
TTGATTTGTGTATCCCTCTT+TGG 0.260850 5_1:+35570626 Msa0729100:CDS
CTTTAGCTTCTGGGATATTT+GGG 0.267158 5_1:+35570534 Msa0729100:CDS
CTTCTGGGATATTTGGGATT+TGG 0.279862 5_1:+35570540 Msa0729100:CDS
TGTTATGACGCCATCTTAAT+TGG 0.285745 5_1:-35572674 None:intergenic
TCCTGTTGAGGAAGAGAATT+AGG 0.298620 5_1:-35570149 None:intergenic
ATTTCTACAGCCTTCATTCA+TGG 0.299138 5_1:+35570597 Msa0729100:CDS
CTCTTTCTTGCGAGAGTTTG+TGG 0.301862 5_1:+35570062 Msa0729100:CDS
GCAGTGGCTTCTTGAACTTT+TGG 0.301868 5_1:+35572277 Msa0729100:intron
AAGGGGTGGCTTTAGCTTCT+GGG 0.303519 5_1:+35570525 Msa0729100:CDS
GTTGTAAATTCTTTAGGCCT+TGG 0.309741 5_1:+35572468 Msa0729100:three_prime_UTR
AGCATTTCTTGTTCTCTTCT+GGG 0.319777 5_1:+35570094 Msa0729100:CDS
TACTACCTTCTCCACTTAAA+AGG 0.321243 5_1:-35570312 None:intergenic
CCACAATCCCATCATTTACT+TGG 0.326307 5_1:-35570571 None:intergenic
TCCATGGTTGTAAATTCTTT+AGG 0.339651 5_1:+35572462 Msa0729100:three_prime_UTR
CATCCTGTGCTCATGGTCAT+TGG 0.339718 5_1:+35570283 Msa0729100:CDS
ACATTTAGTTACAGTTGTTA+TGG 0.357021 5_1:+35570010 Msa0729100:exon
TCTTGTTCTCTTCTGGGCTC+TGG 0.357755 5_1:+35570100 Msa0729100:CDS
GCAGATCTTAGAATAGAAAA+TGG 0.360436 5_1:-35569737 None:intergenic
GCAGTAGCAACGGCGGAAAC+TGG 0.361598 5_1:+35572378 Msa0729100:CDS
TTGTTAGAATTTAGATATCT+TGG 0.364277 5_1:+35572644 Msa0729100:three_prime_UTR
TCCTAATTCTCTTCCTCAAC+AGG 0.404001 5_1:+35570148 Msa0729100:CDS
TGTATCCCTCTTTGGAGCTC+AGG 0.406685 5_1:+35570634 Msa0729100:CDS
TAGATGGTTGCCTGGGTCAA+GGG 0.416984 5_1:+35570472 Msa0729100:CDS
CTGCCAATGCATAAGTGTAT+AGG 0.426996 5_1:-35572359 None:intergenic
ACAAAATTCCAAGTAAATGA+TGG 0.437217 5_1:+35570563 Msa0729100:CDS
TTCTATTCTAAGATCTGCAA+TGG 0.437930 5_1:+35569742 Msa0729100:five_prime_UTR
TACAGCCTTCATTCATGGAT+GGG 0.445813 5_1:+35570602 Msa0729100:CDS
CATGGACTCAGTGCTGTGTT+TGG 0.448440 5_1:-35572445 None:intergenic
ATAGATGGTTGCCTGGGTCA+AGG 0.449560 5_1:+35570471 Msa0729100:CDS
GCGGCGACAACGGTCAGATC+TGG 0.449819 5_1:+35569831 Msa0729100:five_prime_UTR
GAAGAGAATTAGGAGTATTG+AGG 0.459271 5_1:-35570139 None:intergenic
AAATGCGATCAGTGAGGATA+AGG 0.461868 5_1:+35572310 Msa0729100:CDS
TCGGTGCATTTATGTCTTCA+AGG 0.466772 5_1:+35570506 Msa0729100:CDS
TTGGTGCATAGATGGTTGCC+TGG 0.471390 5_1:+35570464 Msa0729100:CDS
GTAAACAACGATTGTGTTGA+AGG 0.474451 5_1:+35569765 Msa0729100:five_prime_UTR
GGTTCTCCATCCTGTGCTCA+TGG 0.477323 5_1:+35570276 Msa0729100:intron
CTACAGCCTTCATTCATGGA+TGG 0.480089 5_1:+35570601 Msa0729100:CDS
AGATGGTTGCCTGGGTCAAG+GGG 0.483244 5_1:+35570473 Msa0729100:CDS
AAGAGCAAAGGGAGTAGTGA+GGG 0.491250 5_1:-35570044 None:intergenic
CTCTCGCAAGAAAGAGCAAA+GGG 0.500913 5_1:-35570055 None:intergenic
CGGTGCATTTATGTCTTCAA+GGG 0.507370 5_1:+35570507 Msa0729100:CDS
GTCAATTTGGTCGCAAACAA+AGG 0.515334 5_1:+35569682 Msa0729100:five_prime_UTR
TCTATTCTAAGATCTGCAAT+GGG 0.521294 5_1:+35569743 Msa0729100:five_prime_UTR
CAAACACAGCACTGAGTCCA+TGG 0.530906 5_1:+35572446 Msa0729100:exon
CAAAATTCCAAGTAAATGAT+GGG 0.535853 5_1:+35570564 Msa0729100:CDS
CCAAGTAAATGATGGGATTG+TGG 0.538655 5_1:+35570571 Msa0729100:CDS
TCAAACCCATCCATGAATGA+AGG 0.539092 5_1:-35570607 None:intergenic
GGATCTAAGAGCCAGGTTGT+CGG 0.540019 5_1:+35569918 Msa0729100:five_prime_UTR
GTGTAATACTCCCTCCGTCC+CGG 0.540277 5_1:-35569562 None:intergenic
GTGGATGGCTGCCGACAACC+TGG 0.544797 5_1:-35569929 None:intergenic
GCATTTATGTCTTCAAGGGG+TGG 0.549961 5_1:+35570511 Msa0729100:CDS
TGGCCTATACACTTATGCAT+TGG 0.552843 5_1:+35572356 Msa0729100:CDS
GTCAGTTATTCCGGGACGGA+GGG 0.558569 5_1:+35569552 None:intergenic
TGGTCACAAGCCAATTAAGA+TGG 0.558924 5_1:+35572664 Msa0729100:three_prime_UTR
TCACTGATCGCATTTCTCCT+CGG 0.562616 5_1:-35572302 None:intergenic
CTTGGTCTTGCTCTCCTCAG+TGG 0.566596 5_1:+35572486 Msa0729100:three_prime_UTR
AGGCCAAGAAATACATGCCA+AGG 0.572983 5_1:-35572339 None:intergenic
TGGTGCATAGATGGTTGCCT+GGG 0.586545 5_1:+35570465 Msa0729100:CDS
TTATGCATTGGCAGTAGCAA+CGG 0.591173 5_1:+35572368 Msa0729100:CDS
GGTCAGTTATTCCGGGACGG+AGG 0.593751 5_1:+35569551 None:intergenic
GCCTAAAGAATTTACAACCA+TGG 0.598613 5_1:-35572463 None:intergenic
AATCCAATGACCATGAGCAC+AGG 0.602677 5_1:-35570286 None:intergenic
GTTGCTAAACGCGGCGACAA+CGG 0.605493 5_1:+35569821 Msa0729100:five_prime_UTR
CTCACTACTAAATACACTCG+TGG 0.610031 5_1:-35569948 None:intergenic
CAATGACCATGAGCACAGGA+TGG 0.614301 5_1:-35570282 None:intergenic
CTACTAAATACACTCGTGGA+TGG 0.619730 5_1:-35569944 None:intergenic
TGAGGATAAGGATCTTACCT+TGG 0.620818 5_1:+35572322 Msa0729100:CDS
GATGGTTGCCTGGGTCAAGG+GGG 0.624454 5_1:+35570474 Msa0729100:CDS
AATGGTATCATACTGACAGA+AGG 0.626636 5_1:-35569719 None:intergenic
ACTCTCGCAAGAAAGAGCAA+AGG 0.634673 5_1:-35570056 None:intergenic
GAAGCAGTGGTTGCTAAACG+CGG 0.634869 5_1:+35569812 Msa0729100:five_prime_UTR
ATGGGTCAGTTATTCCGGGA+CGG 0.635699 5_1:+35569548 None:intergenic
TACTCACCTGAGCTCCAAAG+AGG 0.638028 5_1:-35570640 None:intergenic
ACTCACCTGAGCTCCAAAGA+GGG 0.638153 5_1:-35570639 None:intergenic
TGCATTGGCAGTAGCAACGG+CGG 0.640059 5_1:+35572371 Msa0729100:CDS
CACTGAGGAGAGCAAGACCA+AGG 0.641031 5_1:-35572485 None:intergenic
GTGAGTGAGTGACTAGTGAG+AGG 0.642048 5_1:+35569966 Msa0729100:five_prime_UTR
CCAAAATAACAAAGCCACTG+AGG 0.646081 5_1:-35572500 None:intergenic
GGTGCATTTATGTCTTCAAG+GGG 0.646132 5_1:+35570508 Msa0729100:CDS
AAAGAGCAAAGGGAGTAGTG+AGG 0.647530 5_1:-35570045 None:intergenic
TAAACAACGATTGTGTTGAA+GGG 0.648525 5_1:+35569766 Msa0729100:five_prime_UTR
GCATAGATGAGATCCTGTTG+AGG 0.669004 5_1:-35570161 None:intergenic
GAGTAGTGAGGGAAAGACAG+TGG 0.669481 5_1:-35570033 None:intergenic
TAAATGTTGTGCAGAAGCAG+TGG 0.679941 5_1:+35569799 Msa0729100:five_prime_UTR
GAGGAGAAATGCGATCAGTG+AGG 0.738861 5_1:+35572304 Msa0729100:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTGATGTAATTAAATAAAAA+GGG - chr5_1:35570217-35570236 None:intergenic 10.0%
!! TTTGATGTAATTAAATAAAA+AGG - chr5_1:35570218-35570237 None:intergenic 10.0%
!!! TTTAATTTTAATTTAATTGC+AGG + chr5_1:35570255-35570274 Msa0729100:intron 10.0%
!!! TTCTAAATTAATTTTTACTT+AGG + chr5_1:35570701-35570720 Msa0729100:intron 10.0%
!! AATAATTTGATAAAAACTAA+AGG + chr5_1:35570874-35570893 Msa0729100:intron 10.0%
!! AATAATTTGATAAAAACTAA+AGG - chr5_1:35570874-35570893 None:intergenic 10.0%
!! GATAATAACAATTCATAATT+GGG - chr5_1:35571935-35571954 None:intergenic 15.0%
!! TGATAATAACAATTCATAAT+TGG - chr5_1:35571936-35571955 None:intergenic 15.0%
!! AACCCAAAAATATTATGTAT+TGG + chr5_1:35570348-35570367 Msa0729100:intron 20.0%
!! ACCCAAAAATATTATGTATT+GGG + chr5_1:35570349-35570368 Msa0729100:intron 20.0%
!!! TCCCAATACATAATATTTTT+GGG - chr5_1:35570353-35570372 None:intergenic 20.0%
!!! ATCCCAATACATAATATTTT+TGG - chr5_1:35570354-35570373 None:intergenic 20.0%
!! AAATATTATGTATTGGGATT+AGG + chr5_1:35570355-35570374 Msa0729100:intron 20.0%
!! TAATGAAATCAAGTCATTAT+TGG + chr5_1:35570388-35570407 Msa0729100:intron 20.0%
!! AATACATGTATGTTAATTGA+TGG + chr5_1:35570776-35570795 Msa0729100:intron 20.0%
!!! TATGTTAACTGATGTATTTT+TGG + chr5_1:35571569-35571588 Msa0729100:intron 20.0%
!! AGCAACTAAATTGAAATAAA+AGG - chr5_1:35571865-35571884 None:intergenic 20.0%
!! ACACAATTAATTTCTATCAA+TGG - chr5_1:35572010-35572029 None:intergenic 20.0%
!!! GCAAAATTCTTTTATGTATA+TGG + chr5_1:35572201-35572220 Msa0729100:intron 20.0%
!!! TTTTGAAGAATTTGATACTA+AGG - chr5_1:35572537-35572556 None:intergenic 20.0%
!! TTGTTAGAATTTAGATATCT+TGG + chr5_1:35572644-35572663 Msa0729100:three_prime_UTR 20.0%
! ACAACAAAGCAAAAAACTAA+AGG - chr5_1:35569641-35569660 None:intergenic 25.0%
!! TTTGCTTTGTTGTAATTCTA+TGG + chr5_1:35569648-35569667 Msa0729100:five_prime_UTR 25.0%
! ACATTTAGTTACAGTTGTTA+TGG + chr5_1:35570010-35570029 Msa0729100:exon 25.0%
!! ATTGGATTTATCCTTTTAAG+TGG + chr5_1:35570301-35570320 Msa0729100:CDS 25.0%
! ACAAAATTCCAAGTAAATGA+TGG + chr5_1:35570563-35570582 Msa0729100:CDS 25.0%
! CAAAATTCCAAGTAAATGAT+GGG + chr5_1:35570564-35570583 Msa0729100:CDS 25.0%
!!! TTAATTGATGGTGATTTTGT+TGG + chr5_1:35570788-35570807 Msa0729100:intron 25.0%
!! TTTTATGGTGAACTTTACAA+TGG - chr5_1:35570949-35570968 None:intergenic 25.0%
!!! TGAGAGTGTATTGTTTTTTA+TGG - chr5_1:35571130-35571149 None:intergenic 25.0%
! AATGCATATATGTTAAGTGA+TGG - chr5_1:35571317-35571336 None:intergenic 25.0%
! TTAATTGATTGCGATATTGT+CGG - chr5_1:35571342-35571361 None:intergenic 25.0%
!! TATGTTGATTGATTGTGATA+TGG - chr5_1:35571419-35571438 None:intergenic 25.0%
! TTGATTGATTGTGATATTGT+TGG - chr5_1:35571448-35571467 None:intergenic 25.0%
! TATTTCTCACAATTTAAGTG+AGG + chr5_1:35571515-35571534 Msa0729100:intron 25.0%
! ATTTCTCACAATTTAAGTGA+GGG + chr5_1:35571516-35571535 Msa0729100:intron 25.0%
! AACAATAAGAAACTGGAATT+TGG - chr5_1:35571840-35571859 None:intergenic 25.0%
! AAAGGATAACAATAAGAAAC+TGG - chr5_1:35571847-35571866 None:intergenic 25.0%
! GGCTTTTACTTATGTCAATT+TGG + chr5_1:35569669-35569688 Msa0729100:five_prime_UTR 30.0%
GCAGATCTTAGAATAGAAAA+TGG - chr5_1:35569740-35569759 None:intergenic 30.0%
!! TTCTATTCTAAGATCTGCAA+TGG + chr5_1:35569742-35569761 Msa0729100:five_prime_UTR 30.0%
!! TCTATTCTAAGATCTGCAAT+GGG + chr5_1:35569743-35569762 Msa0729100:five_prime_UTR 30.0%
TAAACAACGATTGTGTTGAA+GGG + chr5_1:35569766-35569785 Msa0729100:five_prime_UTR 30.0%
AGAAAAAGAAAGAGAGAAAG+AGG - chr5_1:35569873-35569892 None:intergenic 30.0%
!!! ATCCACTTTTTCTTCCATAA+CGG - chr5_1:35569902-35569921 None:intergenic 30.0%
AAAGGGAATGAAGAAAATAG+AGG - chr5_1:35570200-35570219 None:intergenic 30.0%
!! TTTATCCTTTTAAGTGGAGA+AGG + chr5_1:35570307-35570326 Msa0729100:CDS 30.0%
ATGACTTAAATTGGAGATAG+AGG - chr5_1:35571090-35571109 None:intergenic 30.0%
TCTATCTCCAATTTAAGTCA+TGG + chr5_1:35571003-35571022 Msa0729100:intron 30.0%
! ACTTTTGCCATGACTTAAAT+TGG - chr5_1:35571081-35571100 None:intergenic 30.0%
ATTTAAGTCATGGCAAAAGT+AGG + chr5_1:35571013-35571032 Msa0729100:intron 30.0%
ATTTAAGTCATGGCAAAAGT+AGG - chr5_1:35571013-35571032 None:intergenic 30.0%
! ACTTTTGCCATGACTTAAAT+TGG + chr5_1:35571081-35571100 Msa0729100:intron 30.0%
TCTATCTCCAATTTAAGTCA+TGG - chr5_1:35571003-35571022 None:intergenic 30.0%
ATGACTTAAATTGGAGATAG+AGG + chr5_1:35571090-35571109 Msa0729100:intron 30.0%
!! TTAAGTGATGGAGATTTTGT+TGG - chr5_1:35571305-35571324 None:intergenic 30.0%
TTGATTGATTGCGATATTGT+CGG - chr5_1:35571378-35571397 None:intergenic 30.0%
TTGATTGATTGTGATATGGT+TGG - chr5_1:35571415-35571434 None:intergenic 30.0%
TTTCTCACAATTTAAGTGAG+GGG + chr5_1:35571517-35571536 Msa0729100:intron 30.0%
TACTTACTGTATACATAGTG+TGG - chr5_1:35571551-35571570 None:intergenic 30.0%
TCAGGACATACATGAATAAA+GGG - chr5_1:35571890-35571909 None:intergenic 30.0%
ATCAGGACATACATGAATAA+AGG - chr5_1:35571891-35571910 None:intergenic 30.0%
! TGAATTGTTATTATCACCAC+TGG + chr5_1:35571940-35571959 Msa0729100:intron 30.0%
TATATGCACAGCAATAACAA+GGG - chr5_1:35572042-35572061 None:intergenic 30.0%
TTATATGCACAGCAATAACA+AGG - chr5_1:35572043-35572062 None:intergenic 30.0%
TCCATGGTTGTAAATTCTTT+AGG + chr5_1:35572462-35572481 Msa0729100:three_prime_UTR 30.0%
! AACTTTTGTACTCAGATACT+AGG + chr5_1:35572559-35572578 Msa0729100:three_prime_UTR 30.0%
AATGGTATCATACTGACAGA+AGG - chr5_1:35569722-35569741 None:intergenic 35.0%
GTAAACAACGATTGTGTTGA+AGG + chr5_1:35569765-35569784 Msa0729100:five_prime_UTR 35.0%
!! CTTTTTCTGAAAACCCGTTA+TGG + chr5_1:35569885-35569904 Msa0729100:five_prime_UTR 35.0%
!!! TCCACTTTTTCTTCCATAAC+GGG - chr5_1:35569901-35569920 None:intergenic 35.0%
TAGCATTTCTTGTTCTCTTC+TGG + chr5_1:35570093-35570112 Msa0729100:CDS 35.0%
AGCATTTCTTGTTCTCTTCT+GGG + chr5_1:35570094-35570113 Msa0729100:CDS 35.0%
!! GAAGAGAATTAGGAGTATTG+AGG - chr5_1:35570142-35570161 None:intergenic 35.0%
TACTACCTTCTCCACTTAAA+AGG - chr5_1:35570315-35570334 None:intergenic 35.0%
! GAATGTTTGTGCAGCAATTT+TGG + chr5_1:35570445-35570464 Msa0729100:intron 35.0%
!! CTTTAGCTTCTGGGATATTT+GGG + chr5_1:35570534-35570553 Msa0729100:CDS 35.0%
ATTTCTACAGCCTTCATTCA+TGG + chr5_1:35570597-35570616 Msa0729100:CDS 35.0%
!! TTGATTTGTGTATCCCTCTT+TGG + chr5_1:35570626-35570645 Msa0729100:CDS 35.0%
TGGAGATAGAGGAAAAAAGA+AGG - chr5_1:35571101-35571120 None:intergenic 35.0%
GCAAAAGTAGGAATTAGTGT+TGG + chr5_1:35571025-35571044 Msa0729100:intron 35.0%
TGGAGATAGAGGAAAAAAGA+AGG + chr5_1:35571101-35571120 Msa0729100:intron 35.0%
!!! GTATTTTGGTCAGTTTGACT+GGG - chr5_1:35571692-35571711 None:intergenic 35.0%
! TAACTAACGCATGGGTATTT+TGG - chr5_1:35571706-35571725 None:intergenic 35.0%
AATCGCTACGTGAATTCAAA+TGG - chr5_1:35571810-35571829 None:intergenic 35.0%
CAGGACATACATGAATAAAG+GGG - chr5_1:35571889-35571908 None:intergenic 35.0%
ATATGCACAGCAATAACAAG+GGG - chr5_1:35572041-35572060 None:intergenic 35.0%
GGAAATTGTAAACTAGTGTG+AGG + chr5_1:35572083-35572102 Msa0729100:intron 35.0%
CAACATGTTGAACTTCGTTA+TGG - chr5_1:35572253-35572272 None:intergenic 35.0%
TTACCTTGGCATGTATTTCT+TGG + chr5_1:35572336-35572355 Msa0729100:CDS 35.0%
GCCTAAAGAATTTACAACCA+TGG - chr5_1:35572466-35572485 None:intergenic 35.0%
GTTGTAAATTCTTTAGGCCT+TGG + chr5_1:35572468-35572487 Msa0729100:three_prime_UTR 35.0%
TGTTATGACGCCATCTTAAT+TGG - chr5_1:35572677-35572696 None:intergenic 35.0%
GTCAATTTGGTCGCAAACAA+AGG + chr5_1:35569682-35569701 Msa0729100:five_prime_UTR 40.0%
TAAATGTTGTGCAGAAGCAG+TGG + chr5_1:35569799-35569818 Msa0729100:five_prime_UTR 40.0%
ACCCGTTATGGAAGAAAAAG+TGG + chr5_1:35569897-35569916 Msa0729100:five_prime_UTR 40.0%
AAAAAGTGGATCTAAGAGCC+AGG + chr5_1:35569911-35569930 Msa0729100:five_prime_UTR 40.0%
CTACTAAATACACTCGTGGA+TGG - chr5_1:35569947-35569966 None:intergenic 40.0%
CTCACTACTAAATACACTCG+TGG - chr5_1:35569951-35569970 None:intergenic 40.0%
TCCTAATTCTCTTCCTCAAC+AGG + chr5_1:35570148-35570167 Msa0729100:CDS 40.0%
TCCTGTTGAGGAAGAGAATT+AGG - chr5_1:35570152-35570171 None:intergenic 40.0%
!! CAGCAATTTTGGTGCATAGA+TGG + chr5_1:35570456-35570475 Msa0729100:intron 40.0%
TCGGTGCATTTATGTCTTCA+AGG + chr5_1:35570506-35570525 Msa0729100:CDS 40.0%
CGGTGCATTTATGTCTTCAA+GGG + chr5_1:35570507-35570526 Msa0729100:CDS 40.0%
GGTGCATTTATGTCTTCAAG+GGG + chr5_1:35570508-35570527 Msa0729100:CDS 40.0%
!! GCTTTAGCTTCTGGGATATT+TGG + chr5_1:35570533-35570552 Msa0729100:CDS 40.0%
!! CTTCTGGGATATTTGGGATT+TGG + chr5_1:35570540-35570559 Msa0729100:CDS 40.0%
CCACAATCCCATCATTTACT+TGG - chr5_1:35570574-35570593 None:intergenic 40.0%
! CCAAGTAAATGATGGGATTG+TGG + chr5_1:35570571-35570590 Msa0729100:CDS 40.0%
TACAGCCTTCATTCATGGAT+GGG + chr5_1:35570602-35570621 Msa0729100:CDS 40.0%
TCAAACCCATCCATGAATGA+AGG - chr5_1:35570610-35570629 None:intergenic 40.0%
GCAAAAGTAGGAGTTAGTGT+TGG - chr5_1:35571069-35571088 None:intergenic 40.0%
!!! GGTATTTTGGTCAGTTTGAC+TGG - chr5_1:35571693-35571712 None:intergenic 40.0%
GCGTTAGTTAACTAACGCAT+GGG - chr5_1:35571714-35571733 None:intergenic 40.0%
TTGCGTGAGTTAACTTACAC+TGG + chr5_1:35571765-35571784 Msa0729100:intron 40.0%
! AAGTGAAAAGCGCAACAATC+AGG - chr5_1:35571908-35571927 None:intergenic 40.0%
TCAAAAAGTCAAGCCTTGCA+CGG - chr5_1:35571978-35571997 None:intergenic 40.0%
! AACTGCTGTAGCTGTTCTTT+TGG + chr5_1:35572062-35572081 Msa0729100:intron 40.0%
TGCGTTAGTTAACTAACGCA+AGG + chr5_1:35572129-35572148 Msa0729100:intron 40.0%
GCGTTAGTTAACTAACGCAA+GGG + chr5_1:35572130-35572149 Msa0729100:intron 40.0%
!!! AACTAACGCAAGGGCATTTT+TGG + chr5_1:35572139-35572158 Msa0729100:intron 40.0%
!!! GCATTTTTGGCATGGCTAAA+AGG + chr5_1:35572152-35572171 Msa0729100:intron 40.0%
TTCAACATGTTGCATTGCAG+TGG + chr5_1:35572261-35572280 Msa0729100:intron 40.0%
AAATGCGATCAGTGAGGATA+AGG + chr5_1:35572310-35572329 Msa0729100:CDS 40.0%
TGAGGATAAGGATCTTACCT+TGG + chr5_1:35572322-35572341 Msa0729100:CDS 40.0%
TGGCCTATACACTTATGCAT+TGG + chr5_1:35572356-35572375 Msa0729100:CDS 40.0%
CTGCCAATGCATAAGTGTAT+AGG - chr5_1:35572362-35572381 None:intergenic 40.0%
! TTATGCATTGGCAGTAGCAA+CGG + chr5_1:35572368-35572387 Msa0729100:CDS 40.0%
CCAAAATAACAAAGCCACTG+AGG - chr5_1:35572503-35572522 None:intergenic 40.0%
!!! CCTCAGTGGCTTTGTTATTT+TGG + chr5_1:35572500-35572519 Msa0729100:three_prime_UTR 40.0%
TGGTCACAAGCCAATTAAGA+TGG + chr5_1:35572664-35572683 Msa0729100:three_prime_UTR 40.0%
AAGAGCAAAGGGAGTAGTGA+GGG - chr5_1:35570047-35570066 None:intergenic 45.0%
AAAGAGCAAAGGGAGTAGTG+AGG - chr5_1:35570048-35570067 None:intergenic 45.0%
CTCTCGCAAGAAAGAGCAAA+GGG - chr5_1:35570058-35570077 None:intergenic 45.0%
ACTCTCGCAAGAAAGAGCAA+AGG - chr5_1:35570059-35570078 None:intergenic 45.0%
CTCTTTCTTGCGAGAGTTTG+TGG + chr5_1:35570062-35570081 Msa0729100:CDS 45.0%
GCATAGATGAGATCCTGTTG+AGG - chr5_1:35570164-35570183 None:intergenic 45.0%
AATCCAATGACCATGAGCAC+AGG - chr5_1:35570289-35570308 None:intergenic 45.0%
GTCAAGGGGGTTGAAGAAAT+CGG + chr5_1:35570487-35570506 Msa0729100:CDS 45.0%
GCATTTATGTCTTCAAGGGG+TGG + chr5_1:35570511-35570530 Msa0729100:CDS 45.0%
CTACAGCCTTCATTCATGGA+TGG + chr5_1:35570601-35570620 Msa0729100:CDS 45.0%
TTTGGTCAGTTTGACTGGGT+GGG - chr5_1:35571688-35571707 None:intergenic 45.0%
! TTTTGGTCAGTTTGACTGGG+TGG - chr5_1:35571689-35571708 None:intergenic 45.0%
GGCGTTAGTTAACTAACGCA+TGG - chr5_1:35571715-35571734 None:intergenic 45.0%
ACGGTATATTACGACACCAG+TGG - chr5_1:35571959-35571978 None:intergenic 45.0%
GTGTCGTAATATACCGTGCA+AGG + chr5_1:35571962-35571981 Msa0729100:intron 45.0%
! GCAGTGGCTTCTTGAACTTT+TGG + chr5_1:35572277-35572296 Msa0729100:intron 45.0%
! TTCTTGAACTTTTGGCACCG+AGG + chr5_1:35572285-35572304 Msa0729100:CDS 45.0%
TCACTGATCGCATTTCTCCT+CGG - chr5_1:35572305-35572324 None:intergenic 45.0%
AGGCCAAGAAATACATGCCA+AGG - chr5_1:35572342-35572361 None:intergenic 45.0%
GAAGCAGTGGTTGCTAAACG+CGG + chr5_1:35569812-35569831 Msa0729100:five_prime_UTR 50.0%
GGATCTAAGAGCCAGGTTGT+CGG + chr5_1:35569918-35569937 Msa0729100:five_prime_UTR 50.0%
GTGAGTGAGTGACTAGTGAG+AGG + chr5_1:35569966-35569985 Msa0729100:five_prime_UTR 50.0%
GAGTAGTGAGGGAAAGACAG+TGG - chr5_1:35570036-35570055 None:intergenic 50.0%
TCTTGTTCTCTTCTGGGCTC+TGG + chr5_1:35570100-35570119 Msa0729100:CDS 50.0%
CAATGACCATGAGCACAGGA+TGG - chr5_1:35570285-35570304 None:intergenic 50.0%
CATCCTGTGCTCATGGTCAT+TGG + chr5_1:35570283-35570302 Msa0729100:CDS 50.0%
TTGGTGCATAGATGGTTGCC+TGG + chr5_1:35570464-35570483 Msa0729100:CDS 50.0%
TGGTGCATAGATGGTTGCCT+GGG + chr5_1:35570465-35570484 Msa0729100:CDS 50.0%
ATAGATGGTTGCCTGGGTCA+AGG + chr5_1:35570471-35570490 Msa0729100:CDS 50.0%
TAGATGGTTGCCTGGGTCAA+GGG + chr5_1:35570472-35570491 Msa0729100:CDS 50.0%
!! AAGGGGTGGCTTTAGCTTCT+GGG + chr5_1:35570525-35570544 Msa0729100:CDS 50.0%
!! TGTATCCCTCTTTGGAGCTC+AGG + chr5_1:35570634-35570653 Msa0729100:CDS 50.0%
ACTCACCTGAGCTCCAAAGA+GGG - chr5_1:35570642-35570661 None:intergenic 50.0%
TACTCACCTGAGCTCCAAAG+AGG - chr5_1:35570643-35570662 None:intergenic 50.0%
! CCAGCGTGAGTTAAGTTGCA+CGG - chr5_1:35571736-35571755 None:intergenic 50.0%
GCACAGCAATAACAAGGGGT+TGG - chr5_1:35572037-35572056 None:intergenic 50.0%
!!! ACGCAAGGGCATTTTTGGCA+TGG + chr5_1:35572144-35572163 Msa0729100:intron 50.0%
! AAGGCGCGAGTGAAAAGTGT+AGG + chr5_1:35572171-35572190 Msa0729100:intron 50.0%
! AGGCGCGAGTGAAAAGTGTA+GGG + chr5_1:35572172-35572191 Msa0729100:intron 50.0%
GAGGAGAAATGCGATCAGTG+AGG + chr5_1:35572304-35572323 Msa0729100:CDS 50.0%
CATGGACTCAGTGCTGTGTT+TGG - chr5_1:35572448-35572467 None:intergenic 50.0%
! CAAACACAGCACTGAGTCCA+TGG + chr5_1:35572446-35572465 Msa0729100:exon 50.0%
GTTGCTAAACGCGGCGACAA+CGG + chr5_1:35569821-35569840 Msa0729100:five_prime_UTR 55.0%
GGTTCTCCATCCTGTGCTCA+TGG + chr5_1:35570276-35570295 Msa0729100:intron 55.0%
AGATGGTTGCCTGGGTCAAG+GGG + chr5_1:35570473-35570492 Msa0729100:CDS 55.0%
TTCTTCAACCCCCTTGACCC+AGG - chr5_1:35570485-35570504 None:intergenic 55.0%
!! CAAGGGGTGGCTTTAGCTTC+TGG + chr5_1:35570524-35570543 Msa0729100:CDS 55.0%
! TGGGAGCGAAAAGTGCAGAG+GGG - chr5_1:35571669-35571688 None:intergenic 55.0%
! GTGGGAGCGAAAAGTGCAGA+GGG - chr5_1:35571670-35571689 None:intergenic 55.0%
CCGTGCAACTTAACTCACGC+TGG + chr5_1:35571733-35571752 Msa0729100:intron 55.0%
! GGCGCGAGTGAAAAGTGTAG+GGG + chr5_1:35572173-35572192 Msa0729100:intron 55.0%
! GCGCGAGTGAAAAGTGTAGG+GGG + chr5_1:35572174-35572193 Msa0729100:intron 55.0%
! TGCATTGGCAGTAGCAACGG+CGG + chr5_1:35572371-35572390 Msa0729100:CDS 55.0%
CACTGAGGAGAGCAAGACCA+AGG - chr5_1:35572488-35572507 None:intergenic 55.0%
!! CTTGGTCTTGCTCTCCTCAG+TGG + chr5_1:35572486-35572505 Msa0729100:three_prime_UTR 55.0%
GATGGTTGCCTGGGTCAAGG+GGG + chr5_1:35570474-35570493 Msa0729100:CDS 60.0%
! GGTGGGAGCGAAAAGTGCAG+AGG - chr5_1:35571671-35571690 None:intergenic 60.0%
GCAGTAGCAACGGCGGAAAC+TGG + chr5_1:35572378-35572397 Msa0729100:CDS 60.0%
GCGGCGACAACGGTCAGATC+TGG + chr5_1:35569831-35569850 Msa0729100:five_prime_UTR 65.0%
GTGGATGGCTGCCGACAACC+TGG - chr5_1:35569932-35569951 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 35569563 35572713 35569563 ID=Msa0729100;Name=Msa0729100
chr5_1 mRNA 35569563 35572713 35569563 ID=Msa0729100-mRNA-1;Parent=Msa0729100;Name=Msa0729100-mRNA-1;_AED=0.24;_eAED=0.24;_QI=466|1|1|1|1|1|4|253|194
chr5_1 exon 35569563 35570184 35569563 ID=Msa0729100-mRNA-1:exon:10991;Parent=Msa0729100-mRNA-1
chr5_1 exon 35570277 35570328 35570277 ID=Msa0729100-mRNA-1:exon:10992;Parent=Msa0729100-mRNA-1
chr5_1 exon 35570459 35570655 35570459 ID=Msa0729100-mRNA-1:exon:10993;Parent=Msa0729100-mRNA-1
chr5_1 exon 35572281 35572713 35572281 ID=Msa0729100-mRNA-1:exon:10994;Parent=Msa0729100-mRNA-1
chr5_1 five_prime_UTR 35569563 35570028 35569563 ID=Msa0729100-mRNA-1:five_prime_utr;Parent=Msa0729100-mRNA-1
chr5_1 CDS 35570029 35570184 35570029 ID=Msa0729100-mRNA-1:cds;Parent=Msa0729100-mRNA-1
chr5_1 CDS 35570277 35570328 35570277 ID=Msa0729100-mRNA-1:cds;Parent=Msa0729100-mRNA-1
chr5_1 CDS 35570459 35570655 35570459 ID=Msa0729100-mRNA-1:cds;Parent=Msa0729100-mRNA-1
chr5_1 CDS 35572281 35572460 35572281 ID=Msa0729100-mRNA-1:cds;Parent=Msa0729100-mRNA-1
chr5_1 three_prime_UTR 35572461 35572713 35572461 ID=Msa0729100-mRNA-1:three_prime_utr;Parent=Msa0729100-mRNA-1
Gene Sequence

>Msa0729100

ATGGCCACTGTCTTTCCCTCACTACTCCCTTTGCTCTTTCTTGCGAGAGTTTGTGGTCTTTCAGTAGCATTTCTTGTTCTCTTCTGGGCTCTGGCTTTCAAATCTAGCTTCCTCAATACTCCTAATTCTCTTCCTCAACAGGATCTCATCTATGCAGTTCTCCATCCTGTGCTCATGGTCATTGGATTTATCCTTTTAAGTGGAGAAGCAATTTTGGTGCATAGATGGTTGCCTGGGTCAAGGGGGTTGAAGAAATCGGTGCATTTATGTCTTCAAGGGGTGGCTTTAGCTTCTGGGATATTTGGGATTTGGACAAAATTCCAAGTAAATGATGGGATTGTGGCTAATTTCTACAGCCTTCATTCATGGATGGGTTTGATTTGTGTATCCCTCTTTGGAGCTCAGTGGCTTCTTGAACTTTTGGCACCGAGGAGAAATGCGATCAGTGAGGATAAGGATCTTACCTTGGCATGTATTTCTTGGCCTATACACTTATGCATTGGCAGTAGCAACGGCGGAAACTGGATTATTAGAGAAGCTGACATTCTTGCAAACACAGAGAAATGTATCCAAACACAGCACTGA
Protein sequence

>Msa0729100

MATVFPSLLPLLFLARVCGLSVAFLVLFWALAFKSSFLNTPNSLPQQDLIYAVLHPVLMVIGFILLSGEAILVHRWLPGSRGLKKSVHLCLQGVALASGIFGIWTKFQVNDGIVANFYSLHSWMGLICVSLFGAQWLLELLAPRRNAISEDKDLTLACISWPIHLCIGSSNGGNWIIREADILANTEKCIQTQH*