AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0766930

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0766930 MtrunA17_Chr5g0422371 96.429 140 5 0 2 141 4 143 8.20e-93 271
Msa0766930 MtrunA17_Chr1g0203371 40.714 140 75 4 1 138 1 134 8.37e-24 94.0
Msa0766930 MtrunA17_Chr6g0456901 39.706 136 77 3 1 135 1 132 3.19e-23 92.8
Msa0766930 MtrunA17_Chr1g0161921 38.168 131 74 3 14 142 15 140 3.67e-22 89.4
Msa0766930 MtrunA17_Chr8g0375991 36.434 129 75 3 14 140 9 132 2.76e-17 76.6
Msa0766930 MtrunA17_Chr1g0161941 31.538 130 84 4 14 142 9 134 1.42e-14 69.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0766930 AT1G26100.1 59.859 142 46 1 1 142 1 131 6.09e-51 164
Msa0766930 AT5G38630.1 44.275 131 64 5 9 136 9 133 1.09e-26 102
Msa0766930 AT4G25570.2 39.552 134 76 3 9 141 49 178 1.20e-24 97.8
Msa0766930 AT4G25570.1 39.552 134 76 3 9 141 8 137 2.01e-24 96.3
Msa0766930 AT1G14730.1 30.556 144 90 4 14 152 20 158 7.22e-13 65.1

Find 92 sgRNAs with CRISPR-Local

Find 190 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTCAGTGGCTTTGTTATTT+TGG 0.056164 5_2:-29344512 Msa0766930:three_prime_UTR
GCTTTAGCTTCTGGGATATT+TGG 0.112597 5_2:-29346503 Msa0766930:CDS
TAGCATTTCTTGTTCTCTTC+TGG 0.118815 5_2:-29346943 Msa0766930:CDS
CAAGGGGTGGCTTTAGCTTC+TGG 0.175443 5_2:-29346512 Msa0766930:CDS
GAATGTTTGTGCAGCAATTT+TGG 0.224729 5_2:-29346591 Msa0766930:intron
TTACCTTGGCATGTATTTCT+TGG 0.229367 5_2:-29344676 Msa0766930:CDS
TTTGCTTTGTTGTAATTCTA+TGG 0.245295 5_2:-29347388 Msa0766930:five_prime_UTR
TTGATTTGTGTATCCCTCTT+TGG 0.260850 5_2:-29346410 Msa0766930:CDS
CTTTAGCTTCTGGGATATTT+GGG 0.267158 5_2:-29346502 Msa0766930:CDS
CTTCTGGGATATTTGGGATT+TGG 0.279862 5_2:-29346496 Msa0766930:CDS
TGTTATGACGCCATCTTAAT+TGG 0.285745 5_2:+29344338 None:intergenic
TCCTGTTGAGGAAGAGAATT+AGG 0.298620 5_2:+29346887 None:intergenic
ATTTCTACAGCCTTCATTCA+TGG 0.299138 5_2:-29346439 Msa0766930:CDS
CTCTTTCTTGCGAGAGTTTG+TGG 0.301862 5_2:-29346974 Msa0766930:CDS
GCAGTGGCTTCTTGAACTTT+TGG 0.301868 5_2:-29344735 Msa0766930:intron
AAGGGGTGGCTTTAGCTTCT+GGG 0.303519 5_2:-29346511 Msa0766930:CDS
GTTGTAAATTCTTTAGGCCT+TGG 0.309741 5_2:-29344544 Msa0766930:three_prime_UTR
AGCATTTCTTGTTCTCTTCT+GGG 0.319777 5_2:-29346942 Msa0766930:CDS
TACTACCTTCTCCACTTAAA+AGG 0.321243 5_2:+29346724 None:intergenic
CCACAATCCCATCATTTACT+TGG 0.326307 5_2:+29346465 None:intergenic
TCCATGGTTGTAAATTCTTT+AGG 0.339651 5_2:-29344550 Msa0766930:three_prime_UTR
CATCCTGTGCTCATGGTCAT+TGG 0.339718 5_2:-29346753 Msa0766930:CDS
ACATTTAGTTACAGTTGTTA+TGG 0.357021 5_2:-29347026 Msa0766930:exon
TCTTGTTCTCTTCTGGGCTC+TGG 0.357755 5_2:-29346936 Msa0766930:CDS
GCAGATCTTAGAATAGAAAA+TGG 0.360436 5_2:+29347299 None:intergenic
GCAGTAGCAACGGCGGAAAC+TGG 0.361598 5_2:-29344634 Msa0766930:CDS
TTGTTAGAATTTAGATATCT+TGG 0.364277 5_2:-29344368 Msa0766930:three_prime_UTR
TCCTAATTCTCTTCCTCAAC+AGG 0.404001 5_2:-29346888 Msa0766930:CDS
TGTATCCCTCTTTGGAGCTC+AGG 0.406685 5_2:-29346402 Msa0766930:intron
TAGATGGTTGCCTGGGTCAA+GGG 0.416984 5_2:-29346564 Msa0766930:CDS
CTGCCAATGCATAAGTGTAT+AGG 0.426996 5_2:+29344653 None:intergenic
ACAAAATTCCAAGTAAATGA+TGG 0.437217 5_2:-29346473 Msa0766930:CDS
TTCTATTCTAAGATCTGCAA+TGG 0.437930 5_2:-29347294 Msa0766930:five_prime_UTR
TACAGCCTTCATTCATGGAT+GGG 0.445813 5_2:-29346434 Msa0766930:CDS
CATGGACTCAGTGCTGTGTT+TGG 0.448440 5_2:+29344567 None:intergenic
ATAGATGGTTGCCTGGGTCA+AGG 0.449560 5_2:-29346565 Msa0766930:CDS
GCGGCGACAACGGTCAGATC+TGG 0.449819 5_2:-29347205 Msa0766930:five_prime_UTR
GAAGAGAATTAGGAGTATTG+AGG 0.459271 5_2:+29346897 None:intergenic
AAATGCGATCAGTGAGGATA+AGG 0.461868 5_2:-29344702 Msa0766930:CDS
TCGGTGCATTTATGTCTTCA+AGG 0.466772 5_2:-29346530 Msa0766930:CDS
TTGGTGCATAGATGGTTGCC+TGG 0.471390 5_2:-29346572 Msa0766930:CDS
GTAAACAACGATTGTGTTGA+AGG 0.474451 5_2:-29347271 Msa0766930:five_prime_UTR
GGTTCTCCATCCTGTGCTCA+TGG 0.477323 5_2:-29346760 Msa0766930:CDS
CTACAGCCTTCATTCATGGA+TGG 0.480089 5_2:-29346435 Msa0766930:CDS
AGATGGTTGCCTGGGTCAAG+GGG 0.483244 5_2:-29346563 Msa0766930:CDS
AAGAGCAAAGGGAGTAGTGA+GGG 0.491250 5_2:+29346992 None:intergenic
CTCTCGCAAGAAAGAGCAAA+GGG 0.500913 5_2:+29346981 None:intergenic
CGGTGCATTTATGTCTTCAA+GGG 0.507370 5_2:-29346529 Msa0766930:CDS
GTCAATTTGGTCGCAAACAA+AGG 0.515334 5_2:-29347354 Msa0766930:five_prime_UTR
TCTATTCTAAGATCTGCAAT+GGG 0.521294 5_2:-29347293 Msa0766930:five_prime_UTR
CAAACACAGCACTGAGTCCA+TGG 0.530906 5_2:-29344566 Msa0766930:exon
CAAAATTCCAAGTAAATGAT+GGG 0.535853 5_2:-29346472 Msa0766930:CDS
CCAAGTAAATGATGGGATTG+TGG 0.538655 5_2:-29346465 Msa0766930:CDS
TCAAACCCATCCATGAATGA+AGG 0.539092 5_2:+29346429 None:intergenic
GGATCTAAGAGCCAGGTTGT+CGG 0.540019 5_2:-29347118 Msa0766930:five_prime_UTR
GTGTAATACTCCCTCCGTCC+CGG 0.540277 5_2:+29347474 None:intergenic
GTGGATGGCTGCCGACAACC+TGG 0.544797 5_2:+29347107 None:intergenic
GCATTTATGTCTTCAAGGGG+TGG 0.549961 5_2:-29346525 Msa0766930:CDS
TGGCCTATACACTTATGCAT+TGG 0.552843 5_2:-29344656 Msa0766930:CDS
GTCAGTTATTCCGGGACGGA+GGG 0.558569 5_2:-29347484 None:intergenic
TGGTCACAAGCCAATTAAGA+TGG 0.558924 5_2:-29344348 Msa0766930:three_prime_UTR
TCACTGATCGCATTTCTCCT+CGG 0.562616 5_2:+29344710 None:intergenic
CTTGGTCTTGCTCTCCTCAG+TGG 0.566596 5_2:-29344526 Msa0766930:three_prime_UTR
AGGCCAAGAAATACATGCCA+AGG 0.572983 5_2:+29344673 None:intergenic
TGGTGCATAGATGGTTGCCT+GGG 0.586545 5_2:-29346571 Msa0766930:CDS
TTATGCATTGGCAGTAGCAA+CGG 0.591173 5_2:-29344644 Msa0766930:CDS
GGTCAGTTATTCCGGGACGG+AGG 0.593751 5_2:-29347485 None:intergenic
GCCTAAAGAATTTACAACCA+TGG 0.598613 5_2:+29344549 None:intergenic
AATCCAATGACCATGAGCAC+AGG 0.602677 5_2:+29346750 None:intergenic
GTTGCTAAACGCGGCGACAA+CGG 0.605493 5_2:-29347215 Msa0766930:five_prime_UTR
CTCACTACTAAATACACTCG+TGG 0.610031 5_2:+29347088 None:intergenic
CAATGACCATGAGCACAGGA+TGG 0.614301 5_2:+29346754 None:intergenic
CTACTAAATACACTCGTGGA+TGG 0.619730 5_2:+29347092 None:intergenic
TGAGGATAAGGATCTTACCT+TGG 0.620818 5_2:-29344690 Msa0766930:CDS
GATGGTTGCCTGGGTCAAGG+GGG 0.624454 5_2:-29346562 Msa0766930:CDS
AATGGTATCATACTGACAGA+AGG 0.626636 5_2:+29347317 None:intergenic
ACTCTCGCAAGAAAGAGCAA+AGG 0.634673 5_2:+29346980 None:intergenic
GAAGCAGTGGTTGCTAAACG+CGG 0.634869 5_2:-29347224 Msa0766930:five_prime_UTR
ATGGGTCAGTTATTCCGGGA+CGG 0.635699 5_2:-29347488 None:intergenic
TACTCACCTGAGCTCCAAAG+AGG 0.638028 5_2:+29346396 None:intergenic
ACTCACCTGAGCTCCAAAGA+GGG 0.638153 5_2:+29346397 None:intergenic
TGCATTGGCAGTAGCAACGG+CGG 0.640059 5_2:-29344641 Msa0766930:CDS
CACTGAGGAGAGCAAGACCA+AGG 0.641031 5_2:+29344527 None:intergenic
GTGAGTGAGTGACTAGTGAG+AGG 0.642048 5_2:-29347070 Msa0766930:five_prime_UTR
CCAAAATAACAAAGCCACTG+AGG 0.646081 5_2:+29344512 None:intergenic
GGTGCATTTATGTCTTCAAG+GGG 0.646132 5_2:-29346528 Msa0766930:CDS
AAAGAGCAAAGGGAGTAGTG+AGG 0.647530 5_2:+29346991 None:intergenic
TAAACAACGATTGTGTTGAA+GGG 0.648525 5_2:-29347270 Msa0766930:five_prime_UTR
GCATAGATGAGATCCTGTTG+AGG 0.669004 5_2:+29346875 None:intergenic
GAGTAGTGAGGGAAAGACAG+TGG 0.669481 5_2:+29347003 None:intergenic
TAAATGTTGTGCAGAAGCAG+TGG 0.679941 5_2:-29347237 Msa0766930:five_prime_UTR
GAGGAGAAATGCGATCAGTG+AGG 0.738861 5_2:-29344708 Msa0766930:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTGATGTAATTAAATAAAAA+GGG + chr5_2:29344975-29344994 None:intergenic 10.0%
!! TTTGATGTAATTAAATAAAA+AGG + chr5_2:29344976-29344995 None:intergenic 10.0%
!!! TTTAATTTTAATTTAATTGC+AGG - chr5_2:29345013-29345032 Msa0766930:intron 10.0%
!!! TTCTAAATTAATTTTTACTT+AGG - chr5_2:29345459-29345478 Msa0766930:intron 10.0%
!! AATAATTTGATAAAAACTAA+AGG - chr5_2:29345632-29345651 Msa0766930:intron 10.0%
!! AATAATTTGATAAAAACTAA+AGG + chr5_2:29345632-29345651 None:intergenic 10.0%
!! AATACATATATGTTAATTGA+TGG - chr5_2:29345534-29345553 Msa0766930:intron 15.0%
!! GATAATAACAATTCATAATT+GGG + chr5_2:29346717-29346736 None:intergenic 15.0%
!! TGATAATAACAATTCATAAT+TGG + chr5_2:29346718-29346737 None:intergenic 15.0%
!! AACCCAAAAATATTATGTAT+TGG - chr5_2:29345106-29345125 Msa0766930:intron 20.0%
!! ACCCAAAAATATTATGTATT+GGG - chr5_2:29345107-29345126 Msa0766930:intron 20.0%
!!! TCCCAATACATAATATTTTT+GGG + chr5_2:29345111-29345130 None:intergenic 20.0%
!!! ATCCCAATACATAATATTTT+TGG + chr5_2:29345112-29345131 None:intergenic 20.0%
!! AAATATTATGTATTGGGATT+AGG - chr5_2:29345113-29345132 Msa0766930:intron 20.0%
!! TAATGAAATCAAGTCATTAT+TGG - chr5_2:29345146-29345165 Msa0766930:intron 20.0%
!! AGCAACTAAATTGAAATAAA+AGG + chr5_2:29346647-29346666 None:intergenic 20.0%
!! ACACAATTAATTTCTATCAA+TGG + chr5_2:29346792-29346811 None:intergenic 20.0%
!!! GCAAAATTCTTTTATGTATA+TGG - chr5_2:29346983-29347002 Msa0766930:CDS 20.0%
!!! TTTTGAAGAATTTGATACTA+AGG + chr5_2:29347319-29347338 None:intergenic 20.0%
!! TTGTTAGAATTTAGATATCT+TGG - chr5_2:29347426-29347445 Msa0766930:five_prime_UTR 20.0%
! ACAACAAAGCAAAAAACTAA+AGG + chr5_2:29344399-29344418 None:intergenic 25.0%
!! TTTGCTTTGTTGTAATTCTA+TGG - chr5_2:29344406-29344425 Msa0766930:three_prime_UTR 25.0%
! ACATTTAGTTACAGTTGTTA+TGG - chr5_2:29344768-29344787 Msa0766930:intron 25.0%
!! ATTGGATTTATCCTTTTAAG+TGG - chr5_2:29345059-29345078 Msa0766930:intron 25.0%
! ACAAAATTCCAAGTAAATGA+TGG - chr5_2:29345321-29345340 Msa0766930:intron 25.0%
! CAAAATTCCAAGTAAATGAT+GGG - chr5_2:29345322-29345341 Msa0766930:intron 25.0%
!!! TTAATTGATGGTGATTTTGT+TGG - chr5_2:29345546-29345565 Msa0766930:intron 25.0%
!! TTTTATGGTGAACTTTACAA+TGG + chr5_2:29345707-29345726 None:intergenic 25.0%
!!! TGAGAGTGTATTGTTTTTTA+TGG + chr5_2:29345897-29345916 None:intergenic 25.0%
! AATGCATATATGTTAAGTGA+TGG + chr5_2:29346085-29346104 None:intergenic 25.0%
! TTAATTGATTGCGATATTGT+CGG + chr5_2:29346110-29346129 None:intergenic 25.0%
!! TATGTTGATTGATTGTGATA+TGG + chr5_2:29346187-29346206 None:intergenic 25.0%
! TTGATTGATTGTGATATTGT+TGG + chr5_2:29346216-29346235 None:intergenic 25.0%
! TATTTCTCACAATTTAAGTG+AGG - chr5_2:29346283-29346302 Msa0766930:intron 25.0%
! ATTTCTCACAATTTAAGTGA+GGG - chr5_2:29346284-29346303 Msa0766930:intron 25.0%
! AACAATAAGAAACTGGAATT+TGG + chr5_2:29346622-29346641 None:intergenic 25.0%
! AAAGGATAACAATAAGAAAC+TGG + chr5_2:29346629-29346648 None:intergenic 25.0%
! GGCTTTTACTTATGTCAATT+TGG - chr5_2:29344427-29344446 Msa0766930:three_prime_UTR 30.0%
GCAGATCTTAGAATAGAAAA+TGG + chr5_2:29344498-29344517 None:intergenic 30.0%
!! TTCTATTCTAAGATCTGCAA+TGG - chr5_2:29344500-29344519 Msa0766930:three_prime_UTR 30.0%
!! TCTATTCTAAGATCTGCAAT+GGG - chr5_2:29344501-29344520 Msa0766930:three_prime_UTR 30.0%
TAAACAACGATTGTGTTGAA+GGG - chr5_2:29344524-29344543 Msa0766930:three_prime_UTR 30.0%
AGAAAAAGAAAGAGAGAAAG+AGG + chr5_2:29344631-29344650 None:intergenic 30.0%
!!! ATCCACTTTTTCTTCCATAA+CGG + chr5_2:29344660-29344679 None:intergenic 30.0%
AAAGGGAATGAAGAAAATAG+AGG + chr5_2:29344958-29344977 None:intergenic 30.0%
!! TTTATCCTTTTAAGTGGAGA+AGG - chr5_2:29345065-29345084 Msa0766930:intron 30.0%
ATGACTTAAATTGGAGATAG+AGG + chr5_2:29345857-29345876 None:intergenic 30.0%
TCTATCTCCAATTTAAGTCA+TGG - chr5_2:29345761-29345780 Msa0766930:intron 30.0%
! ACTTTTGCCATGACTTAAAT+TGG + chr5_2:29345848-29345867 None:intergenic 30.0%
ATTTAAGTCATGGCAAAAGT+AGG - chr5_2:29345771-29345790 Msa0766930:intron 30.0%
ATTTAAGTCATGGCAAAAGT+AGG + chr5_2:29345771-29345790 None:intergenic 30.0%
! ACTTTTGCCATGACTTAAAT+TGG - chr5_2:29345848-29345867 Msa0766930:intron 30.0%
TCTATCTCCAATTTAAGTCA+TGG + chr5_2:29345761-29345780 None:intergenic 30.0%
ATGACTTAAATTGGAGATAG+AGG - chr5_2:29345857-29345876 Msa0766930:intron 30.0%
!! TTAAGTGATGGAGATTTTGT+TGG + chr5_2:29346073-29346092 None:intergenic 30.0%
TTGATTGATTGCGATATTGT+CGG + chr5_2:29346146-29346165 None:intergenic 30.0%
TTGATTGATTGTGATATGGT+TGG + chr5_2:29346183-29346202 None:intergenic 30.0%
TTTCTCACAATTTAAGTGAG+GGG - chr5_2:29346285-29346304 Msa0766930:intron 30.0%
TACTTACTGTATACATAGTG+TGG + chr5_2:29346319-29346338 None:intergenic 30.0%
TCAGGACATACATGAATAAA+GGG + chr5_2:29346672-29346691 None:intergenic 30.0%
ATCAGGACATACATGAATAA+AGG + chr5_2:29346673-29346692 None:intergenic 30.0%
! TGAATTGTTATTATCACCAC+TGG - chr5_2:29346722-29346741 Msa0766930:intron 30.0%
TATATGCACAGCAATAACAA+GGG + chr5_2:29346824-29346843 None:intergenic 30.0%
TTATATGCACAGCAATAACA+AGG + chr5_2:29346825-29346844 None:intergenic 30.0%
TCCATGGTTGTAAATTCTTT+AGG - chr5_2:29347244-29347263 Msa0766930:five_prime_UTR 30.0%
! AACTTTTGTACTCAGATACT+AGG - chr5_2:29347341-29347360 Msa0766930:five_prime_UTR 30.0%
AATGGTATCATACTGACAGA+AGG + chr5_2:29344480-29344499 None:intergenic 35.0%
GTAAACAACGATTGTGTTGA+AGG - chr5_2:29344523-29344542 Msa0766930:three_prime_UTR 35.0%
!! CTTTTTCTGAAAACCCGTTA+TGG - chr5_2:29344643-29344662 Msa0766930:CDS 35.0%
!!! TCCACTTTTTCTTCCATAAC+GGG + chr5_2:29344659-29344678 None:intergenic 35.0%
TAGCATTTCTTGTTCTCTTC+TGG - chr5_2:29344851-29344870 Msa0766930:intron 35.0%
AGCATTTCTTGTTCTCTTCT+GGG - chr5_2:29344852-29344871 Msa0766930:intron 35.0%
!! GAAGAGAATTAGGAGTATTG+AGG + chr5_2:29344900-29344919 None:intergenic 35.0%
TACTACCTTCTCCACTTAAA+AGG + chr5_2:29345073-29345092 None:intergenic 35.0%
! GAATGTTTGTGCAGCAATTT+TGG - chr5_2:29345203-29345222 Msa0766930:intron 35.0%
!! CTTTAGCTTCTGGGATATTT+GGG - chr5_2:29345292-29345311 Msa0766930:intron 35.0%
ATTTCTACAGCCTTCATTCA+TGG - chr5_2:29345355-29345374 Msa0766930:intron 35.0%
!! TTGATTTGTGTATCCCTCTT+TGG - chr5_2:29345384-29345403 Msa0766930:intron 35.0%
TGGAGATAGAGGAAAAAAGA+AGG + chr5_2:29345868-29345887 None:intergenic 35.0%
GCAAAAGTAGGAATTAGTGT+TGG - chr5_2:29345783-29345802 Msa0766930:intron 35.0%
!! AGTGTTGGTCTGAAGTAATA+CGG + chr5_2:29345821-29345840 None:intergenic 35.0%
TGGAGATAGAGGAAAAAAGA+AGG - chr5_2:29345868-29345887 Msa0766930:intron 35.0%
! TAACTAACGCATGGGTATTT+TGG + chr5_2:29346474-29346493 None:intergenic 35.0%
AATCGCTACGTGAATTCAAA+TGG + chr5_2:29346592-29346611 None:intergenic 35.0%
CAGGACATACATGAATAAAG+GGG + chr5_2:29346671-29346690 None:intergenic 35.0%
ATATGCACAGCAATAACAAG+GGG + chr5_2:29346823-29346842 None:intergenic 35.0%
GGAAATTGTAAACTAGTGTG+AGG - chr5_2:29346865-29346884 Msa0766930:intron 35.0%
CAACATGTTGAACTTCGTTA+TGG + chr5_2:29347035-29347054 None:intergenic 35.0%
TTACCTTGGCATGTATTTCT+TGG - chr5_2:29347118-29347137 Msa0766930:five_prime_UTR 35.0%
GCCTAAAGAATTTACAACCA+TGG + chr5_2:29347248-29347267 None:intergenic 35.0%
GTTGTAAATTCTTTAGGCCT+TGG - chr5_2:29347250-29347269 Msa0766930:five_prime_UTR 35.0%
TGTTATGACGCCATCTTAAT+TGG + chr5_2:29347459-29347478 None:intergenic 35.0%
GTCAATTTGGTCGCAAACAA+AGG - chr5_2:29344440-29344459 Msa0766930:three_prime_UTR 40.0%
TAAATGTTGTGCAGAAGCAG+TGG - chr5_2:29344557-29344576 Msa0766930:exon 40.0%
ACCCGTTATGGAAGAAAAAG+TGG - chr5_2:29344655-29344674 Msa0766930:CDS 40.0%
AAAAAGTGGATCTAAGAGCC+AGG - chr5_2:29344669-29344688 Msa0766930:CDS 40.0%
CTACTAAATACACTCGTGGA+TGG + chr5_2:29344705-29344724 None:intergenic 40.0%
CTCACTACTAAATACACTCG+TGG + chr5_2:29344709-29344728 None:intergenic 40.0%
TCCTAATTCTCTTCCTCAAC+AGG - chr5_2:29344906-29344925 Msa0766930:intron 40.0%
TCCTGTTGAGGAAGAGAATT+AGG + chr5_2:29344910-29344929 None:intergenic 40.0%
!! CAGCAATTTTGGTGCATAGA+TGG - chr5_2:29345214-29345233 Msa0766930:intron 40.0%
TCGGTGCATTTATGTCTTCA+AGG - chr5_2:29345264-29345283 Msa0766930:intron 40.0%
CGGTGCATTTATGTCTTCAA+GGG - chr5_2:29345265-29345284 Msa0766930:intron 40.0%
GGTGCATTTATGTCTTCAAG+GGG - chr5_2:29345266-29345285 Msa0766930:intron 40.0%
!! GCTTTAGCTTCTGGGATATT+TGG - chr5_2:29345291-29345310 Msa0766930:intron 40.0%
!! CTTCTGGGATATTTGGGATT+TGG - chr5_2:29345298-29345317 Msa0766930:intron 40.0%
CCACAATCCCATCATTTACT+TGG + chr5_2:29345332-29345351 None:intergenic 40.0%
! CCAAGTAAATGATGGGATTG+TGG - chr5_2:29345329-29345348 Msa0766930:intron 40.0%
TACAGCCTTCATTCATGGAT+GGG - chr5_2:29345360-29345379 Msa0766930:intron 40.0%
TCAAACCCATCCATGAATGA+AGG + chr5_2:29345368-29345387 None:intergenic 40.0%
GCAAAAGTAGGAGTTAGTGT+TGG + chr5_2:29345836-29345855 None:intergenic 40.0%
TTTGGTCAGTTTGACTGGAT+GGG + chr5_2:29346456-29346475 None:intergenic 40.0%
! TTTTGGTCAGTTTGACTGGA+TGG + chr5_2:29346457-29346476 None:intergenic 40.0%
!!! GGTATTTTGGTCAGTTTGAC+TGG + chr5_2:29346461-29346480 None:intergenic 40.0%
GCGTTAGTTAACTAACGCAT+GGG + chr5_2:29346482-29346501 None:intergenic 40.0%
TTGCGTGAGTTAACTTACAC+TGG - chr5_2:29346533-29346552 Msa0766930:CDS 40.0%
! AAGTGAAAAGCGCAACAATC+AGG + chr5_2:29346690-29346709 None:intergenic 40.0%
TCAAAAAGTCAAGCCTTGCA+CGG + chr5_2:29346760-29346779 None:intergenic 40.0%
! AACTGCTGTAGCTGTTCTTT+TGG - chr5_2:29346844-29346863 Msa0766930:intron 40.0%
TGCGTTAGTTAACTAACGCA+AGG - chr5_2:29346911-29346930 Msa0766930:CDS 40.0%
GCGTTAGTTAACTAACGCAA+GGG - chr5_2:29346912-29346931 Msa0766930:CDS 40.0%
!!! AACTAACGCAAGGGCATTTT+TGG - chr5_2:29346921-29346940 Msa0766930:CDS 40.0%
!!! GCATTTTTGGCATGGCAAAA+AGG - chr5_2:29346934-29346953 Msa0766930:CDS 40.0%
TTCAACATGTTGCATTGCAG+TGG - chr5_2:29347043-29347062 Msa0766930:five_prime_UTR 40.0%
AAATGCGATCAGTGAGGATA+AGG - chr5_2:29347092-29347111 Msa0766930:five_prime_UTR 40.0%
TGAGGATAAGGATCTTACCT+TGG - chr5_2:29347104-29347123 Msa0766930:five_prime_UTR 40.0%
TGGCCTATACACTTATGCAT+TGG - chr5_2:29347138-29347157 Msa0766930:five_prime_UTR 40.0%
CTGCCAATGCATAAGTGTAT+AGG + chr5_2:29347144-29347163 None:intergenic 40.0%
! TTATGCATTGGCAGTAGCAA+CGG - chr5_2:29347150-29347169 Msa0766930:five_prime_UTR 40.0%
CCAAAATAACAAAGCCACTG+AGG + chr5_2:29347285-29347304 None:intergenic 40.0%
!!! CCTCAGTGGCTTTGTTATTT+TGG - chr5_2:29347282-29347301 Msa0766930:five_prime_UTR 40.0%
TGGTCACAAGCCAATTAAGA+TGG - chr5_2:29347446-29347465 Msa0766930:five_prime_UTR 40.0%
AAGAGCAAAGGGAGTAGTGA+GGG + chr5_2:29344805-29344824 None:intergenic 45.0%
AAAGAGCAAAGGGAGTAGTG+AGG + chr5_2:29344806-29344825 None:intergenic 45.0%
CTCTCGCAAGAAAGAGCAAA+GGG + chr5_2:29344816-29344835 None:intergenic 45.0%
ACTCTCGCAAGAAAGAGCAA+AGG + chr5_2:29344817-29344836 None:intergenic 45.0%
CTCTTTCTTGCGAGAGTTTG+TGG - chr5_2:29344820-29344839 Msa0766930:intron 45.0%
GCATAGATGAGATCCTGTTG+AGG + chr5_2:29344922-29344941 None:intergenic 45.0%
AATCCAATGACCATGAGCAC+AGG + chr5_2:29345047-29345066 None:intergenic 45.0%
GTCAAGGGGGTTGAAGAAAT+CGG - chr5_2:29345245-29345264 Msa0766930:intron 45.0%
GCATTTATGTCTTCAAGGGG+TGG - chr5_2:29345269-29345288 Msa0766930:intron 45.0%
CTACAGCCTTCATTCATGGA+TGG - chr5_2:29345359-29345378 Msa0766930:intron 45.0%
GGCGTTAGTTAACTAACGCA+TGG + chr5_2:29346483-29346502 None:intergenic 45.0%
ACGGTATATTACGACACCAG+TGG + chr5_2:29346741-29346760 None:intergenic 45.0%
GTGTCGTAATATACCGTGCA+AGG - chr5_2:29346744-29346763 Msa0766930:CDS 45.0%
! GCAGTGGCTTCTTGAACTTT+TGG - chr5_2:29347059-29347078 Msa0766930:five_prime_UTR 45.0%
! TTCTTGAACTTTTGGCACCG+AGG - chr5_2:29347067-29347086 Msa0766930:five_prime_UTR 45.0%
TCACTGATCGCATTTCTCCT+CGG + chr5_2:29347087-29347106 None:intergenic 45.0%
AGGCCAAGAAATACATGCCA+AGG + chr5_2:29347124-29347143 None:intergenic 45.0%
GAAGCAGTGGTTGCTAAACG+CGG - chr5_2:29344570-29344589 Msa0766930:exon 50.0%
GGATCTAAGAGCCAGGTTGT+CGG - chr5_2:29344676-29344695 Msa0766930:CDS 50.0%
GTGAGTGAGTGACTAGTGAG+AGG - chr5_2:29344724-29344743 Msa0766930:CDS 50.0%
GAGTAGTGAGGGAAAGACAG+TGG + chr5_2:29344794-29344813 None:intergenic 50.0%
TCTTGTTCTCTTCTGGGCTC+TGG - chr5_2:29344858-29344877 Msa0766930:intron 50.0%
CAATGACCATGAGCACAGGA+TGG + chr5_2:29345043-29345062 None:intergenic 50.0%
CATCCTGTGCTCATGGTCAT+TGG - chr5_2:29345041-29345060 Msa0766930:intron 50.0%
TTGGTGCATAGATGGTTGCC+TGG - chr5_2:29345222-29345241 Msa0766930:intron 50.0%
TGGTGCATAGATGGTTGCCT+GGG - chr5_2:29345223-29345242 Msa0766930:intron 50.0%
ATAGATGGTTGCCTGGGTCA+AGG - chr5_2:29345229-29345248 Msa0766930:intron 50.0%
TAGATGGTTGCCTGGGTCAA+GGG - chr5_2:29345230-29345249 Msa0766930:intron 50.0%
!! AAGGGGTGGCTTTAGCTTCT+GGG - chr5_2:29345283-29345302 Msa0766930:intron 50.0%
!! TGTATCCCTCTTTGGAGCTC+AGG - chr5_2:29345392-29345411 Msa0766930:intron 50.0%
ACTCACCTGAGCTCCAAAGA+GGG + chr5_2:29345400-29345419 None:intergenic 50.0%
TACTCACCTGAGCTCCAAAG+AGG + chr5_2:29345401-29345420 None:intergenic 50.0%
! ATGGGAGCGAAAAGTGCAGA+GGG + chr5_2:29346438-29346457 None:intergenic 50.0%
! CCAGCGTGAGTTAAGTTGCA+CGG + chr5_2:29346504-29346523 None:intergenic 50.0%
GCACAGCAATAACAAGGGGT+TGG + chr5_2:29346819-29346838 None:intergenic 50.0%
!!! ACGCAAGGGCATTTTTGGCA+TGG - chr5_2:29346926-29346945 Msa0766930:CDS 50.0%
! AAGGCGCGAGTGAAAAGTGT+AGG - chr5_2:29346953-29346972 Msa0766930:CDS 50.0%
! AGGCGCGAGTGAAAAGTGTA+GGG - chr5_2:29346954-29346973 Msa0766930:CDS 50.0%
GAGGAGAAATGCGATCAGTG+AGG - chr5_2:29347086-29347105 Msa0766930:five_prime_UTR 50.0%
CATGGACTCAGTGCTGTGTT+TGG + chr5_2:29347230-29347249 None:intergenic 50.0%
! CAAACACAGCACTGAGTCCA+TGG - chr5_2:29347228-29347247 Msa0766930:five_prime_UTR 50.0%
GTTGCTAAACGCGGCGACAA+CGG - chr5_2:29344579-29344598 Msa0766930:CDS 55.0%
GGTTCTCCATCCTGTGCTCA+TGG - chr5_2:29345034-29345053 Msa0766930:intron 55.0%
AGATGGTTGCCTGGGTCAAG+GGG - chr5_2:29345231-29345250 Msa0766930:intron 55.0%
TTCTTCAACCCCCTTGACCC+AGG + chr5_2:29345243-29345262 None:intergenic 55.0%
!! CAAGGGGTGGCTTTAGCTTC+TGG - chr5_2:29345282-29345301 Msa0766930:intron 55.0%
! TGGGAGCGAAAAGTGCAGAG+GGG + chr5_2:29346437-29346456 None:intergenic 55.0%
! GATGGGAGCGAAAAGTGCAG+AGG + chr5_2:29346439-29346458 None:intergenic 55.0%
CCGTGCAACTTAACTCACGC+TGG - chr5_2:29346501-29346520 Msa0766930:CDS 55.0%
! GGCGCGAGTGAAAAGTGTAG+GGG - chr5_2:29346955-29346974 Msa0766930:CDS 55.0%
! GCGCGAGTGAAAAGTGTAGG+GGG - chr5_2:29346956-29346975 Msa0766930:CDS 55.0%
! TGCATTGGCAGTAGCAACGG+CGG - chr5_2:29347153-29347172 Msa0766930:five_prime_UTR 55.0%
CACTGAGGAGAGCAAGACCA+AGG + chr5_2:29347270-29347289 None:intergenic 55.0%
!! CTTGGTCTTGCTCTCCTCAG+TGG - chr5_2:29347268-29347287 Msa0766930:five_prime_UTR 55.0%
GATGGTTGCCTGGGTCAAGG+GGG - chr5_2:29345232-29345251 Msa0766930:intron 60.0%
GCAGTAGCAACGGCGGAAAC+TGG - chr5_2:29347160-29347179 Msa0766930:five_prime_UTR 60.0%
GCGGCGACAACGGTCAGATC+TGG - chr5_2:29344589-29344608 Msa0766930:CDS 65.0%
GTGGATGGCTGCCGACAACC+TGG + chr5_2:29344690-29344709 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 29344321 29347495 29344321 ID=Msa0766930;Name=Msa0766930
chr5_2 mRNA 29344321 29347495 29344321 ID=Msa0766930-mRNA-1;Parent=Msa0766930;Name=Msa0766930-mRNA-1;_AED=0.25;_eAED=0.25;_QI=466|1|1|1|1|1|4|253|194
chr5_2 exon 29344321 29344753 29344321 ID=Msa0766930-mRNA-1:exon:11225;Parent=Msa0766930-mRNA-1
chr5_2 exon 29346403 29346599 29346403 ID=Msa0766930-mRNA-1:exon:11224;Parent=Msa0766930-mRNA-1
chr5_2 exon 29346730 29346781 29346730 ID=Msa0766930-mRNA-1:exon:11223;Parent=Msa0766930-mRNA-1
chr5_2 exon 29346874 29347495 29346874 ID=Msa0766930-mRNA-1:exon:11222;Parent=Msa0766930-mRNA-1
chr5_2 five_prime_UTR 29347030 29347495 29347030 ID=Msa0766930-mRNA-1:five_prime_utr;Parent=Msa0766930-mRNA-1
chr5_2 CDS 29346874 29347029 29346874 ID=Msa0766930-mRNA-1:cds;Parent=Msa0766930-mRNA-1
chr5_2 CDS 29346730 29346781 29346730 ID=Msa0766930-mRNA-1:cds;Parent=Msa0766930-mRNA-1
chr5_2 CDS 29346403 29346599 29346403 ID=Msa0766930-mRNA-1:cds;Parent=Msa0766930-mRNA-1
chr5_2 CDS 29344574 29344753 29344574 ID=Msa0766930-mRNA-1:cds;Parent=Msa0766930-mRNA-1
chr5_2 three_prime_UTR 29344321 29344573 29344321 ID=Msa0766930-mRNA-1:three_prime_utr;Parent=Msa0766930-mRNA-1
Gene Sequence

>Msa0766930

ATGGCCACTGTCTTTCCCTCACTACTCCCTTTGCTCTTTCTTGCGAGAGTTTGTGGTCTTTCAGTAGCATTTCTTGTTCTCTTCTGGGCTCTGGCTTTCAAATCTAGCTTCCTCAATACTCCTAATTCTCTTCCTCAACAGGATCTCATCTATGCAGTTCTCCATCCTGTGCTCATGGTCATTGGATTTATCCTTTTAAGTGGAGAAGCAATTTTGGTGCATAGATGGTTGCCTGGGTCAAGGGGGTTGAAGAAATCGGTGCATTTATGTCTTCAAGGGGTGGCTTTAGCTTCTGGGATATTTGGGATTTGGACAAAATTCCAAGTAAATGATGGGATTGTGGCTAATTTCTACAGCCTTCATTCATGGATGGGTTTGATTTGTGTATCCCTCTTTGGAGCTCAGTGGCTTCTTGAACTTTTGGCACCGAGGAGAAATGCGATCAGTGAGGATAAGGATCTTACCTTGGCATGTATTTCTTGGCCTATACACTTATGCATTGGCAGTAGCAACGGCGGAAACTGGATTATTAGAGAAGCTGACATTCTTGCAAACACAGAGAAATGTATCCAAACACAGCACTGA
Protein sequence

>Msa0766930

MATVFPSLLPLLFLARVCGLSVAFLVLFWALAFKSSFLNTPNSLPQQDLIYAVLHPVLMVIGFILLSGEAILVHRWLPGSRGLKKSVHLCLQGVALASGIFGIWTKFQVNDGIVANFYSLHSWMGLICVSLFGAQWLLELLAPRRNAISEDKDLTLACISWPIHLCIGSSNGGNWIIREADILANTEKCIQTQH*