AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0900770

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 48 sgRNAs with CRISPR-Local

Find 296 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATTAAGAACAATCAAAATT+TGG 0.187988 6_1:-96977822 Msa0900770:five_prime_UTR
GAAGAGAAGTGAGACGGTTT+TGG 0.294453 6_1:+96978022 None:intergenic
ACAAGAGGATCATCTAGTTC+TGG 0.312368 6_1:-96975899 Msa0900770:three_prime_UTR
CATTTCTTCCTCTTGCTCCT+TGG 0.325994 6_1:+96976102 None:intergenic
CACTCGAAACTCATCTATAA+AGG 0.344139 6_1:-96977781 Msa0900770:intron
GTAGTGGGAAGAGGTCTTCT+TGG 0.346543 6_1:-96976072 Msa0900770:CDS
AATTCCATTCTCTGCAACTT+TGG 0.390172 6_1:+96976027 None:intergenic
TTGCACCTTGTGTTGGAAAC+TGG 0.401274 6_1:-96976006 Msa0900770:CDS
TGCATTATGTGCAGGTAGCT+TGG 0.407949 6_1:-96975930 Msa0900770:three_prime_UTR
CAAGAGGATCATCTAGTTCT+GGG 0.436940 6_1:-96975898 Msa0900770:three_prime_UTR
ACACAAGAAAGTCCACGAAC+AGG 0.438225 6_1:+96976928 None:intergenic
CGCATTCAAAACCCTGTTCG+TGG 0.447453 6_1:-96976940 Msa0900770:five_prime_UTR
TGTCTGCTAGGAATGTTGTT+GGG 0.450777 6_1:-96975969 Msa0900770:exon
TCATCTCTCTAAAGTTAGTA+AGG 0.464335 6_1:-96976867 Msa0900770:CDS
GGTTCCAAAGTTGCAGAGAA+TGG 0.465975 6_1:-96976031 Msa0900770:CDS
TGGAAGATTGCAGAGAAAGA+AGG 0.486096 6_1:-96976052 Msa0900770:CDS
CACATTCAAAACCATGTTCG+TGG 0.497210 6_1:-96976903 Msa0900770:exon
AGGTATCTAATCAGTACAGT+AGG 0.498460 6_1:-96977035 Msa0900770:five_prime_UTR
GAGGGCGATGGAGCGATGAA+AGG 0.512177 6_1:+96977973 None:intergenic
CTGTCTGCTAGGAATGTTGT+TGG 0.512759 6_1:-96975970 Msa0900770:exon
AAGAAATGATCTTAAGTAGT+GGG 0.519554 6_1:-96976087 Msa0900770:CDS
GTCTGCTAGGAATGTTGTTG+GGG 0.524420 6_1:-96975968 Msa0900770:exon
GAAGAAATGATCTTAAGTAG+TGG 0.525243 6_1:-96976088 Msa0900770:CDS
GGTATCTAATCAGTACAGTA+GGG 0.529874 6_1:-96977034 Msa0900770:five_prime_UTR
TGATCTTAAGTAGTGGGAAG+AGG 0.538774 6_1:-96976081 Msa0900770:CDS
TAGCAACAATACTGTCTGCT+AGG 0.542055 6_1:-96975981 Msa0900770:exon
ATCTGTATAAATCAAATCAA+AGG 0.542988 6_1:-96977010 Msa0900770:five_prime_UTR
AGGGCGATGGAGCGATGAAA+GGG 0.544961 6_1:+96977974 None:intergenic
AAAATACCTTTGTGAGGGAG+AGG 0.545723 6_1:+96977954 None:intergenic
AGAAAGATTGCATTATGTGC+AGG 0.545965 6_1:-96975938 Msa0900770:three_prime_UTR
AATGGAATTGCACCTTGTGT+TGG 0.547259 6_1:-96976013 Msa0900770:CDS
CTTTGTGAGGGAGAGGGCGA+TGG 0.557738 6_1:+96977961 None:intergenic
ATGAAAGGGTTTCCTTCGTG+AGG 0.581808 6_1:+96977988 None:intergenic
TGAAAGGGTTTCCTTCGTGA+GGG 0.582008 6_1:+96977989 None:intergenic
TAACAGGACCAAGGAGCAAG+AGG 0.582357 6_1:-96976110 Msa0900770:intron
ATATTGGTTCTGAGTATGAA+AGG 0.583298 6_1:-96976832 Msa0900770:intron
CTACACCAGTTTCCAACACA+AGG 0.592815 6_1:+96976001 None:intergenic
CACAAGAAAGTCCACGAACA+GGG 0.597521 6_1:+96976929 None:intergenic
AAATACCTTTGTGAGGGAGA+GGG 0.598580 6_1:+96977955 None:intergenic
GAAAGGGTTTCCTTCGTGAG+GGG 0.623252 6_1:+96977990 None:intergenic
TTTCACGAAGAGAAGTGAGA+CGG 0.625027 6_1:+96978016 None:intergenic
TGATAATTATATGTTTGATG+TGG 0.634378 6_1:-96977866 Msa0900770:intron
TTTACTTTATAACAGGACCA+AGG 0.637117 6_1:-96976119 Msa0900770:intron
ACTGAGAAAGTCCACGAACA+TGG 0.644673 6_1:+96976892 None:intergenic
CGTGAAATCTCCCCTCACGA+AGG 0.657109 6_1:-96978000 Msa0900770:five_prime_UTR
CATCGCCCTCTCCCTCACAA+AGG 0.667019 6_1:-96977960 Msa0900770:intron
GTATCTAATCAGTACAGTAG+GGG 0.670712 6_1:-96977033 Msa0900770:five_prime_UTR
AGCTTGGAAAGAGAAACAAG+AGG 0.730779 6_1:-96975914 Msa0900770:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTAATGAAATAAAATAGA+GGG + chr6_1:96975814-96975833 None:intergenic 10.0%
!!! TTTTTAATGAAATAAAATAG+AGG + chr6_1:96975815-96975834 None:intergenic 10.0%
!! TTAAAAAAAATCCATAAAAA+CGG + chr6_1:96976910-96976929 None:intergenic 10.0%
!!! TGATTTTTTTTTATTGTAAT+AGG - chr6_1:96977408-96977427 Msa0900770:intron 10.0%
!!! TTTTAATGAAATAAAATAGA+GGG + chr6_1:96975814-96975833 None:intergenic 10.0%
!!! TTTTTAATGAAATAAAATAG+AGG + chr6_1:96975815-96975834 None:intergenic 10.0%
!! TTAAAAAAAATCCATAAAAA+CGG + chr6_1:96976910-96976929 None:intergenic 10.0%
!!! TGATTTTTTTTTATTGTAAT+AGG - chr6_1:96977408-96977427 Msa0900770:intron 10.0%
!!! TTAATTTTATGATTATGATC+AGG - chr6_1:96976836-96976855 Msa0900770:CDS 15.0%
!!! GTTTTTATGGATTTTTTTTA+AGG - chr6_1:96976909-96976928 Msa0900770:exon 15.0%
!!! AGATTATTATTTGATAGTTT+TGG - chr6_1:96977250-96977269 Msa0900770:intron 15.0%
!!! TTAGTATAAGATTTCATTTT+TGG - chr6_1:96978039-96978058 Msa0900770:five_prime_UTR 15.0%
!!! TTAATTTTATGATTATGATC+AGG - chr6_1:96976836-96976855 Msa0900770:CDS 15.0%
!!! GTTTTTATGGATTTTTTTTA+AGG - chr6_1:96976909-96976928 Msa0900770:exon 15.0%
!!! AGATTATTATTTGATAGTTT+TGG - chr6_1:96977250-96977269 Msa0900770:intron 15.0%
!!! TTAGTATAAGATTTCATTTT+TGG - chr6_1:96978039-96978058 Msa0900770:five_prime_UTR 15.0%
!! ATTAAAACAATTCAAAGACA+GGG + chr6_1:96976000-96976019 None:intergenic 20.0%
!! TGATAATTATATGTTTGATG+TGG - chr6_1:96976025-96976044 Msa0900770:CDS 20.0%
!!! GTTGATTTCAATGAAAAATT+GGG - chr6_1:96976047-96976066 Msa0900770:CDS 20.0%
!! GATTAAGAACAATCAAAATT+TGG - chr6_1:96976069-96976088 Msa0900770:CDS 20.0%
!! ACAAATCAATATTCAAAGAT+GGG + chr6_1:96976152-96976171 None:intergenic 20.0%
!! AACAAATCAATATTCAAAGA+TGG + chr6_1:96976153-96976172 None:intergenic 20.0%
!! TTAATAAGCAAGCATATATA+TGG + chr6_1:96976397-96976416 None:intergenic 20.0%
!! ATCTGTATAAATCAAATCAA+AGG - chr6_1:96976881-96976900 Msa0900770:CDS 20.0%
!!! AGTTAGTAAGGATAAATTTT+TGG - chr6_1:96977036-96977055 Msa0900770:five_prime_UTR 20.0%
!!! AAGGATAAATTTTTGGATAT+TGG - chr6_1:96977043-96977062 Msa0900770:intron 20.0%
!! CTATCAAATAATAATCTTTG+TGG + chr6_1:96977247-96977266 None:intergenic 20.0%
!!! TATTCAATTCTCACATTTTA+TGG - chr6_1:96977304-96977323 Msa0900770:intron 20.0%
!!! TATTGTAATAGGTTGTTTTT+AGG - chr6_1:96977419-96977438 Msa0900770:intron 20.0%
!!! ATTGTAATAGGTTGTTTTTA+GGG - chr6_1:96977420-96977439 Msa0900770:intron 20.0%
!!! AAATTGAAGAGCTTTTAAAA+TGG + chr6_1:96977503-96977522 None:intergenic 20.0%
!!! TCAACGAATTGTTTAAAATA+TGG + chr6_1:96977571-96977590 None:intergenic 20.0%
!! ATTGTTAAAAGTAGATTTGT+TGG - chr6_1:96977597-96977616 Msa0900770:intron 20.0%
!!! TTGTTAAAAGTAGATTTGTT+GGG - chr6_1:96977598-96977617 Msa0900770:intron 20.0%
!!! TATCTGTTATTTTGATTTGT+TGG - chr6_1:96977633-96977652 Msa0900770:intron 20.0%
!!! ATCTGTTATTTTGATTTGTT+GGG - chr6_1:96977634-96977653 Msa0900770:intron 20.0%
!! ATGAGAATTTACTTTATAAC+AGG - chr6_1:96977765-96977784 Msa0900770:intron 20.0%
!! ATTAAAACAATTCAAAGACA+GGG + chr6_1:96976000-96976019 None:intergenic 20.0%
!! TGATAATTATATGTTTGATG+TGG - chr6_1:96976025-96976044 Msa0900770:CDS 20.0%
!!! GTTGATTTCAATGAAAAATT+GGG - chr6_1:96976047-96976066 Msa0900770:CDS 20.0%
!! GATTAAGAACAATCAAAATT+TGG - chr6_1:96976069-96976088 Msa0900770:CDS 20.0%
!! ACAAATCAATATTCAAAGAT+GGG + chr6_1:96976152-96976171 None:intergenic 20.0%
!! AACAAATCAATATTCAAAGA+TGG + chr6_1:96976153-96976172 None:intergenic 20.0%
!! TTAATAAGCAAGCATATATA+TGG + chr6_1:96976397-96976416 None:intergenic 20.0%
!! ATCTGTATAAATCAAATCAA+AGG - chr6_1:96976881-96976900 Msa0900770:CDS 20.0%
!!! AGTTAGTAAGGATAAATTTT+TGG - chr6_1:96977036-96977055 Msa0900770:five_prime_UTR 20.0%
!!! AAGGATAAATTTTTGGATAT+TGG - chr6_1:96977043-96977062 Msa0900770:intron 20.0%
!! CTATCAAATAATAATCTTTG+TGG + chr6_1:96977247-96977266 None:intergenic 20.0%
!!! TATTCAATTCTCACATTTTA+TGG - chr6_1:96977304-96977323 Msa0900770:intron 20.0%
!!! TATTGTAATAGGTTGTTTTT+AGG - chr6_1:96977419-96977438 Msa0900770:intron 20.0%
!!! ATTGTAATAGGTTGTTTTTA+GGG - chr6_1:96977420-96977439 Msa0900770:intron 20.0%
!!! AAATTGAAGAGCTTTTAAAA+TGG + chr6_1:96977503-96977522 None:intergenic 20.0%
!!! TCAACGAATTGTTTAAAATA+TGG + chr6_1:96977571-96977590 None:intergenic 20.0%
!! ATTGTTAAAAGTAGATTTGT+TGG - chr6_1:96977597-96977616 Msa0900770:intron 20.0%
!!! TTGTTAAAAGTAGATTTGTT+GGG - chr6_1:96977598-96977617 Msa0900770:intron 20.0%
!!! TATCTGTTATTTTGATTTGT+TGG - chr6_1:96977633-96977652 Msa0900770:intron 20.0%
!!! ATCTGTTATTTTGATTTGTT+GGG - chr6_1:96977634-96977653 Msa0900770:intron 20.0%
!! ATGAGAATTTACTTTATAAC+AGG - chr6_1:96977765-96977784 Msa0900770:intron 20.0%
! GAATAAAAATACCTTTGTGA+GGG + chr6_1:96975945-96975964 None:intergenic 25.0%
! AGAATAAAAATACCTTTGTG+AGG + chr6_1:96975946-96975965 None:intergenic 25.0%
!! TTCTTTTTCCATAGATCAAT+TGG - chr6_1:96975962-96975981 Msa0900770:three_prime_UTR 25.0%
! CATTAAAACAATTCAAAGAC+AGG + chr6_1:96976001-96976020 None:intergenic 25.0%
!! GGTTGATTTCAATGAAAAAT+TGG - chr6_1:96976046-96976065 Msa0900770:CDS 25.0%
! ATTAGACTAGAATAAGTAGT+TGG + chr6_1:96976300-96976319 None:intergenic 25.0%
! CTACAAAACAAAGTATAGTA+TGG + chr6_1:96976343-96976362 None:intergenic 25.0%
!!! CATACTATACTTTGTTTTGT+AGG - chr6_1:96976341-96976360 Msa0900770:intron 25.0%
! TACTCAAGATTGAATTCAAA+CGG + chr6_1:96976519-96976538 None:intergenic 25.0%
! TGAATTCAATCTTGAGTAAT+TGG - chr6_1:96976521-96976540 Msa0900770:intron 25.0%
!!! AGGCTTATTTGATTTTCTTT+TGG - chr6_1:96976552-96976571 Msa0900770:intron 25.0%
!! TTTTACTCTTTAGTTGAACA+TGG - chr6_1:96976598-96976617 Msa0900770:intron 25.0%
!!! TTAACTTGTAAACTTCATTG+TGG - chr6_1:96976639-96976658 Msa0900770:intron 25.0%
! TAAATCTGAGTCCAAAATAT+GGG - chr6_1:96977197-96977216 Msa0900770:intron 25.0%
! AAATAATAATCTTTGTGGCA+GGG + chr6_1:96977242-96977261 None:intergenic 25.0%
!! TCTCACATTTTATGGTTTTT+TGG - chr6_1:96977312-96977331 Msa0900770:intron 25.0%
!! CTCACATTTTATGGTTTTTT+GGG - chr6_1:96977313-96977332 Msa0900770:intron 25.0%
!!! ATTTTATGGTTTTTTGGGTT+CGG - chr6_1:96977318-96977337 Msa0900770:intron 25.0%
!! TCGTAGTTTTCTGTATTTTT+CGG - chr6_1:96977348-96977367 Msa0900770:intron 25.0%
! AGAAAATCAAGAATGATGAA+CGG + chr6_1:96977387-96977406 None:intergenic 25.0%
! TGGTTCAAATTATAAAAGCT+TGG - chr6_1:96977454-96977473 Msa0900770:intron 25.0%
! TTGGGTTTATAATGAAAGTT+GGG - chr6_1:96977652-96977671 Msa0900770:intron 25.0%
! AAGAAATGATCTTAAGTAGT+GGG - chr6_1:96977804-96977823 Msa0900770:five_prime_UTR 25.0%
! GAATAAAAATACCTTTGTGA+GGG + chr6_1:96975945-96975964 None:intergenic 25.0%
! AGAATAAAAATACCTTTGTG+AGG + chr6_1:96975946-96975965 None:intergenic 25.0%
!! TTCTTTTTCCATAGATCAAT+TGG - chr6_1:96975962-96975981 Msa0900770:three_prime_UTR 25.0%
! CATTAAAACAATTCAAAGAC+AGG + chr6_1:96976001-96976020 None:intergenic 25.0%
!! GGTTGATTTCAATGAAAAAT+TGG - chr6_1:96976046-96976065 Msa0900770:CDS 25.0%
! ATTAGACTAGAATAAGTAGT+TGG + chr6_1:96976300-96976319 None:intergenic 25.0%
! CTACAAAACAAAGTATAGTA+TGG + chr6_1:96976343-96976362 None:intergenic 25.0%
!!! CATACTATACTTTGTTTTGT+AGG - chr6_1:96976341-96976360 Msa0900770:intron 25.0%
! TACTCAAGATTGAATTCAAA+CGG + chr6_1:96976519-96976538 None:intergenic 25.0%
! TGAATTCAATCTTGAGTAAT+TGG - chr6_1:96976521-96976540 Msa0900770:intron 25.0%
!!! AGGCTTATTTGATTTTCTTT+TGG - chr6_1:96976552-96976571 Msa0900770:intron 25.0%
!! TTTTACTCTTTAGTTGAACA+TGG - chr6_1:96976598-96976617 Msa0900770:intron 25.0%
!!! TTAACTTGTAAACTTCATTG+TGG - chr6_1:96976639-96976658 Msa0900770:intron 25.0%
! TAAATCTGAGTCCAAAATAT+GGG - chr6_1:96977197-96977216 Msa0900770:intron 25.0%
! AAATAATAATCTTTGTGGCA+GGG + chr6_1:96977242-96977261 None:intergenic 25.0%
!! TCTCACATTTTATGGTTTTT+TGG - chr6_1:96977312-96977331 Msa0900770:intron 25.0%
!! CTCACATTTTATGGTTTTTT+GGG - chr6_1:96977313-96977332 Msa0900770:intron 25.0%
!!! ATTTTATGGTTTTTTGGGTT+CGG - chr6_1:96977318-96977337 Msa0900770:intron 25.0%
!! TCGTAGTTTTCTGTATTTTT+CGG - chr6_1:96977348-96977367 Msa0900770:intron 25.0%
! AGAAAATCAAGAATGATGAA+CGG + chr6_1:96977387-96977406 None:intergenic 25.0%
! TGGTTCAAATTATAAAAGCT+TGG - chr6_1:96977454-96977473 Msa0900770:intron 25.0%
! TTGGGTTTATAATGAAAGTT+GGG - chr6_1:96977652-96977671 Msa0900770:intron 25.0%
! AAGAAATGATCTTAAGTAGT+GGG - chr6_1:96977804-96977823 Msa0900770:five_prime_UTR 25.0%
TGATAGAACCAATTGATCTA+TGG + chr6_1:96975973-96975992 None:intergenic 30.0%
!!! GGTTTTAGGGTTTTTGATTT+GGG - chr6_1:96976194-96976213 Msa0900770:intron 30.0%
AATTTATGCCAAAGATGCTT+TGG - chr6_1:96976365-96976384 Msa0900770:intron 30.0%
! GAAAGTTCTGATCTTTGATT+TGG - chr6_1:96976453-96976472 Msa0900770:intron 30.0%
AGAAAATCAAATAAGCCTTC+AGG + chr6_1:96976550-96976569 None:intergenic 30.0%
TCTTTAGTTGAACATGGTTA+TGG - chr6_1:96976604-96976623 Msa0900770:intron 30.0%
!! ATCAAAGGAATCCGTTTTTA+TGG - chr6_1:96976896-96976915 Msa0900770:exon 30.0%
!! TCATCTCTCTAAAGTTAGTA+AGG - chr6_1:96977024-96977043 Msa0900770:five_prime_UTR 30.0%
!! ATATTGGTTCTGAGTATGAA+AGG - chr6_1:96977059-96977078 Msa0900770:intron 30.0%
GTAAATCTGAGTCCAAAATA+TGG - chr6_1:96977196-96977215 Msa0900770:intron 30.0%
!!! GCATTTGTTATCCCATATTT+TGG + chr6_1:96977211-96977230 None:intergenic 30.0%
ATATGGGATAACAAATGCTA+AGG - chr6_1:96977213-96977232 Msa0900770:intron 30.0%
AATAATAATCTTTGTGGCAG+GGG + chr6_1:96977241-96977260 None:intergenic 30.0%
CAAATAATAATCTTTGTGGC+AGG + chr6_1:96977243-96977262 None:intergenic 30.0%
!!! TGATAGTTTTGGTAATCTAG+AGG - chr6_1:96977261-96977280 Msa0900770:intron 30.0%
!!! GATAGTTTTGGTAATCTAGA+GGG - chr6_1:96977262-96977281 Msa0900770:intron 30.0%
TAATCTAGAGGGTTAAAGTT+TGG - chr6_1:96977273-96977292 Msa0900770:intron 30.0%
TTAAAATGGACCACACAATA+AGG + chr6_1:96977489-96977508 None:intergenic 30.0%
! CTCTTCAATTTTATTCCTAC+AGG - chr6_1:96977512-96977531 Msa0900770:intron 30.0%
! CAATTTTATTCCTACAGGTA+AGG - chr6_1:96977517-96977536 Msa0900770:intron 30.0%
GTTGGGTTTATAATGAAAGT+TGG - chr6_1:96977651-96977670 Msa0900770:intron 30.0%
CCATTCACAATGTATGAAAA+AGG + chr6_1:96977686-96977705 None:intergenic 30.0%
!! CCTTTTTCATACATTGTGAA+TGG - chr6_1:96977683-96977702 Msa0900770:intron 30.0%
TTTACTTTATAACAGGACCA+AGG - chr6_1:96977772-96977791 Msa0900770:intron 30.0%
GAAGAAATGATCTTAAGTAG+TGG - chr6_1:96977803-96977822 Msa0900770:five_prime_UTR 30.0%
TGATAGAACCAATTGATCTA+TGG + chr6_1:96975973-96975992 None:intergenic 30.0%
!!! GGTTTTAGGGTTTTTGATTT+GGG - chr6_1:96976194-96976213 Msa0900770:intron 30.0%
AATTTATGCCAAAGATGCTT+TGG - chr6_1:96976365-96976384 Msa0900770:intron 30.0%
! GAAAGTTCTGATCTTTGATT+TGG - chr6_1:96976453-96976472 Msa0900770:intron 30.0%
AGAAAATCAAATAAGCCTTC+AGG + chr6_1:96976550-96976569 None:intergenic 30.0%
TCTTTAGTTGAACATGGTTA+TGG - chr6_1:96976604-96976623 Msa0900770:intron 30.0%
!! ATCAAAGGAATCCGTTTTTA+TGG - chr6_1:96976896-96976915 Msa0900770:exon 30.0%
!! TCATCTCTCTAAAGTTAGTA+AGG - chr6_1:96977024-96977043 Msa0900770:five_prime_UTR 30.0%
!! ATATTGGTTCTGAGTATGAA+AGG - chr6_1:96977059-96977078 Msa0900770:intron 30.0%
GTAAATCTGAGTCCAAAATA+TGG - chr6_1:96977196-96977215 Msa0900770:intron 30.0%
!!! GCATTTGTTATCCCATATTT+TGG + chr6_1:96977211-96977230 None:intergenic 30.0%
ATATGGGATAACAAATGCTA+AGG - chr6_1:96977213-96977232 Msa0900770:intron 30.0%
AATAATAATCTTTGTGGCAG+GGG + chr6_1:96977241-96977260 None:intergenic 30.0%
CAAATAATAATCTTTGTGGC+AGG + chr6_1:96977243-96977262 None:intergenic 30.0%
!!! TGATAGTTTTGGTAATCTAG+AGG - chr6_1:96977261-96977280 Msa0900770:intron 30.0%
!!! GATAGTTTTGGTAATCTAGA+GGG - chr6_1:96977262-96977281 Msa0900770:intron 30.0%
TAATCTAGAGGGTTAAAGTT+TGG - chr6_1:96977273-96977292 Msa0900770:intron 30.0%
TTAAAATGGACCACACAATA+AGG + chr6_1:96977489-96977508 None:intergenic 30.0%
! CTCTTCAATTTTATTCCTAC+AGG - chr6_1:96977512-96977531 Msa0900770:intron 30.0%
! CAATTTTATTCCTACAGGTA+AGG - chr6_1:96977517-96977536 Msa0900770:intron 30.0%
GTTGGGTTTATAATGAAAGT+TGG - chr6_1:96977651-96977670 Msa0900770:intron 30.0%
CCATTCACAATGTATGAAAA+AGG + chr6_1:96977686-96977705 None:intergenic 30.0%
!! CCTTTTTCATACATTGTGAA+TGG - chr6_1:96977683-96977702 Msa0900770:intron 30.0%
TTTACTTTATAACAGGACCA+AGG - chr6_1:96977772-96977791 Msa0900770:intron 30.0%
GAAGAAATGATCTTAAGTAG+TGG - chr6_1:96977803-96977822 Msa0900770:five_prime_UTR 30.0%
CACTCGAAACTCATCTATAA+AGG - chr6_1:96976110-96976129 Msa0900770:intron 35.0%
TTGCTGCTAGATTGTGATTT+CGG - chr6_1:96976172-96976191 Msa0900770:intron 35.0%
!!! AGATTGTGATTTCGGGTTTT+AGG - chr6_1:96976180-96976199 Msa0900770:intron 35.0%
!!! GATTGTGATTTCGGGTTTTA+GGG - chr6_1:96976181-96976200 Msa0900770:intron 35.0%
!!! GGGTTTTAGGGTTTTTGATT+TGG - chr6_1:96976193-96976212 Msa0900770:intron 35.0%
!! CTCCTTCAATTTGGGTTTTA+GGG - chr6_1:96976233-96976252 Msa0900770:intron 35.0%
AACCCTAAAACCCAAATTGA+AGG + chr6_1:96976238-96976257 None:intergenic 35.0%
!!! GGTTTTAGGGTTTCTGATTT+GGG - chr6_1:96976246-96976265 Msa0900770:intron 35.0%
!!! GTTTTAGGGTTTCTGATTTG+GGG - chr6_1:96976247-96976266 Msa0900770:intron 35.0%
TTGAGTAATTGGATTCCTGA+AGG - chr6_1:96976532-96976551 Msa0900770:intron 35.0%
GTAAACTTCATTGTGGTCTT+TGG - chr6_1:96976646-96976665 Msa0900770:intron 35.0%
AGGTATCTAATCAGTACAGT+AGG - chr6_1:96976856-96976875 Msa0900770:CDS 35.0%
GGTATCTAATCAGTACAGTA+GGG - chr6_1:96976857-96976876 Msa0900770:CDS 35.0%
GTATCTAATCAGTACAGTAG+GGG - chr6_1:96976858-96976877 Msa0900770:CDS 35.0%
TTGTTACTCCAATCCTGTAA+TGG - chr6_1:96977105-96977124 Msa0900770:intron 35.0%
TGTTACTCCAATCCTGTAAT+GGG - chr6_1:96977106-96977125 Msa0900770:intron 35.0%
CTCAGATTTACATACAAGCA+TGG + chr6_1:96977187-96977206 None:intergenic 35.0%
ATAATAATCTTTGTGGCAGG+GGG + chr6_1:96977240-96977259 None:intergenic 35.0%
CAAGAATGATGAACGGATAA+AGG + chr6_1:96977380-96977399 None:intergenic 35.0%
!!! TAGGTTGTTTTTAGGGCAAT+TGG - chr6_1:96977427-96977446 Msa0900770:intron 35.0%
!!! TTTTTAGGGCAATTGGAAAC+TGG - chr6_1:96977434-96977453 Msa0900770:intron 35.0%
GTTGTGAATACCTTATTGTG+TGG - chr6_1:96977476-96977495 Msa0900770:intron 35.0%
TATCATTGATCCTTACCTGT+AGG + chr6_1:96977530-96977549 None:intergenic 35.0%
AATTCCATTCTCTGCAACTT+TGG + chr6_1:96977867-96977886 None:intergenic 35.0%
AGAAAGATTGCATTATGTGC+AGG - chr6_1:96977953-96977972 Msa0900770:intron 35.0%
CACTCGAAACTCATCTATAA+AGG - chr6_1:96976110-96976129 Msa0900770:intron 35.0%
TTGCTGCTAGATTGTGATTT+CGG - chr6_1:96976172-96976191 Msa0900770:intron 35.0%
!!! AGATTGTGATTTCGGGTTTT+AGG - chr6_1:96976180-96976199 Msa0900770:intron 35.0%
!!! GATTGTGATTTCGGGTTTTA+GGG - chr6_1:96976181-96976200 Msa0900770:intron 35.0%
!!! GGGTTTTAGGGTTTTTGATT+TGG - chr6_1:96976193-96976212 Msa0900770:intron 35.0%
!! CTCCTTCAATTTGGGTTTTA+GGG - chr6_1:96976233-96976252 Msa0900770:intron 35.0%
AACCCTAAAACCCAAATTGA+AGG + chr6_1:96976238-96976257 None:intergenic 35.0%
!!! GGTTTTAGGGTTTCTGATTT+GGG - chr6_1:96976246-96976265 Msa0900770:intron 35.0%
!!! GTTTTAGGGTTTCTGATTTG+GGG - chr6_1:96976247-96976266 Msa0900770:intron 35.0%
TTGAGTAATTGGATTCCTGA+AGG - chr6_1:96976532-96976551 Msa0900770:intron 35.0%
GTAAACTTCATTGTGGTCTT+TGG - chr6_1:96976646-96976665 Msa0900770:intron 35.0%
AGGTATCTAATCAGTACAGT+AGG - chr6_1:96976856-96976875 Msa0900770:CDS 35.0%
GGTATCTAATCAGTACAGTA+GGG - chr6_1:96976857-96976876 Msa0900770:CDS 35.0%
GTATCTAATCAGTACAGTAG+GGG - chr6_1:96976858-96976877 Msa0900770:CDS 35.0%
TTGTTACTCCAATCCTGTAA+TGG - chr6_1:96977105-96977124 Msa0900770:intron 35.0%
TGTTACTCCAATCCTGTAAT+GGG - chr6_1:96977106-96977125 Msa0900770:intron 35.0%
CTCAGATTTACATACAAGCA+TGG + chr6_1:96977187-96977206 None:intergenic 35.0%
ATAATAATCTTTGTGGCAGG+GGG + chr6_1:96977240-96977259 None:intergenic 35.0%
CAAGAATGATGAACGGATAA+AGG + chr6_1:96977380-96977399 None:intergenic 35.0%
!!! TAGGTTGTTTTTAGGGCAAT+TGG - chr6_1:96977427-96977446 Msa0900770:intron 35.0%
!!! TTTTTAGGGCAATTGGAAAC+TGG - chr6_1:96977434-96977453 Msa0900770:intron 35.0%
GTTGTGAATACCTTATTGTG+TGG - chr6_1:96977476-96977495 Msa0900770:intron 35.0%
TATCATTGATCCTTACCTGT+AGG + chr6_1:96977530-96977549 None:intergenic 35.0%
AATTCCATTCTCTGCAACTT+TGG + chr6_1:96977867-96977886 None:intergenic 35.0%
AGAAAGATTGCATTATGTGC+AGG - chr6_1:96977953-96977972 Msa0900770:intron 35.0%
TTTCACGAAGAGAAGTGAGA+CGG + chr6_1:96975878-96975897 None:intergenic 40.0%
AAATACCTTTGTGAGGGAGA+GGG + chr6_1:96975939-96975958 None:intergenic 40.0%
AAAATACCTTTGTGAGGGAG+AGG + chr6_1:96975940-96975959 None:intergenic 40.0%
TGCTGCTAGATTGTGATTTC+GGG - chr6_1:96976173-96976192 Msa0900770:intron 40.0%
ATTCACGTGCTCCTTCAATT+TGG - chr6_1:96976224-96976243 Msa0900770:intron 40.0%
TTCACGTGCTCCTTCAATTT+GGG - chr6_1:96976225-96976244 Msa0900770:intron 40.0%
!! GCTCCTTCAATTTGGGTTTT+AGG - chr6_1:96976232-96976251 Msa0900770:intron 40.0%
!!! GGGTTTTAGGGTTTCTGATT+TGG - chr6_1:96976245-96976264 Msa0900770:intron 40.0%
GTTGAACATGGTTATGGCTA+TGG - chr6_1:96976610-96976629 Msa0900770:intron 40.0%
GTTGTCAAAGAGTCATCACA+TGG + chr6_1:96976794-96976813 None:intergenic 40.0%
CACATTCAAAACCATGTTCG+TGG - chr6_1:96976988-96977007 Msa0900770:five_prime_UTR 40.0%
AATGTTGCCCATTACAGGAT+TGG + chr6_1:96977116-96977135 None:intergenic 40.0%
GACATAATGTTGCCCATTAC+AGG + chr6_1:96977121-96977140 None:intergenic 40.0%
TAATAATCTTTGTGGCAGGG+GGG + chr6_1:96977239-96977258 None:intergenic 40.0%
GAGGGTTAAAGTTTGGAAAG+TGG - chr6_1:96977280-96977299 Msa0900770:intron 40.0%
! TGATCTTAAGTAGTGGGAAG+AGG - chr6_1:96977810-96977829 Msa0900770:five_prime_UTR 40.0%
TGGAAGATTGCAGAGAAAGA+AGG - chr6_1:96977839-96977858 Msa0900770:five_prime_UTR 40.0%
AATGGAATTGCACCTTGTGT+TGG - chr6_1:96977878-96977897 Msa0900770:intron 40.0%
TAGCAACAATACTGTCTGCT+AGG - chr6_1:96977910-96977929 Msa0900770:intron 40.0%
TGTCTGCTAGGAATGTTGTT+GGG - chr6_1:96977922-96977941 Msa0900770:intron 40.0%
AGCTTGGAAAGAGAAACAAG+AGG - chr6_1:96977977-96977996 Msa0900770:five_prime_UTR 40.0%
! ACAAGAGGATCATCTAGTTC+TGG - chr6_1:96977992-96978011 Msa0900770:five_prime_UTR 40.0%
!! CAAGAGGATCATCTAGTTCT+GGG - chr6_1:96977993-96978012 Msa0900770:five_prime_UTR 40.0%
TTTCACGAAGAGAAGTGAGA+CGG + chr6_1:96975878-96975897 None:intergenic 40.0%
AAATACCTTTGTGAGGGAGA+GGG + chr6_1:96975939-96975958 None:intergenic 40.0%
AAAATACCTTTGTGAGGGAG+AGG + chr6_1:96975940-96975959 None:intergenic 40.0%
TGCTGCTAGATTGTGATTTC+GGG - chr6_1:96976173-96976192 Msa0900770:intron 40.0%
ATTCACGTGCTCCTTCAATT+TGG - chr6_1:96976224-96976243 Msa0900770:intron 40.0%
TTCACGTGCTCCTTCAATTT+GGG - chr6_1:96976225-96976244 Msa0900770:intron 40.0%
!! GCTCCTTCAATTTGGGTTTT+AGG - chr6_1:96976232-96976251 Msa0900770:intron 40.0%
!!! GGGTTTTAGGGTTTCTGATT+TGG - chr6_1:96976245-96976264 Msa0900770:intron 40.0%
GTTGAACATGGTTATGGCTA+TGG - chr6_1:96976610-96976629 Msa0900770:intron 40.0%
GTTGTCAAAGAGTCATCACA+TGG + chr6_1:96976794-96976813 None:intergenic 40.0%
CACATTCAAAACCATGTTCG+TGG - chr6_1:96976988-96977007 Msa0900770:five_prime_UTR 40.0%
AATGTTGCCCATTACAGGAT+TGG + chr6_1:96977116-96977135 None:intergenic 40.0%
GACATAATGTTGCCCATTAC+AGG + chr6_1:96977121-96977140 None:intergenic 40.0%
TAATAATCTTTGTGGCAGGG+GGG + chr6_1:96977239-96977258 None:intergenic 40.0%
GAGGGTTAAAGTTTGGAAAG+TGG - chr6_1:96977280-96977299 Msa0900770:intron 40.0%
! TGATCTTAAGTAGTGGGAAG+AGG - chr6_1:96977810-96977829 Msa0900770:five_prime_UTR 40.0%
TGGAAGATTGCAGAGAAAGA+AGG - chr6_1:96977839-96977858 Msa0900770:five_prime_UTR 40.0%
AATGGAATTGCACCTTGTGT+TGG - chr6_1:96977878-96977897 Msa0900770:intron 40.0%
TAGCAACAATACTGTCTGCT+AGG - chr6_1:96977910-96977929 Msa0900770:intron 40.0%
TGTCTGCTAGGAATGTTGTT+GGG - chr6_1:96977922-96977941 Msa0900770:intron 40.0%
AGCTTGGAAAGAGAAACAAG+AGG - chr6_1:96977977-96977996 Msa0900770:five_prime_UTR 40.0%
! ACAAGAGGATCATCTAGTTC+TGG - chr6_1:96977992-96978011 Msa0900770:five_prime_UTR 40.0%
!! CAAGAGGATCATCTAGTTCT+GGG - chr6_1:96977993-96978012 Msa0900770:five_prime_UTR 40.0%
! GAAGAGAAGTGAGACGGTTT+TGG + chr6_1:96975872-96975891 None:intergenic 45.0%
TGAAAGGGTTTCCTTCGTGA+GGG + chr6_1:96975905-96975924 None:intergenic 45.0%
ATGAAAGGGTTTCCTTCGTG+AGG + chr6_1:96975906-96975925 None:intergenic 45.0%
! GGACTTTGCCAAAGCATCTT+TGG + chr6_1:96976376-96976395 None:intergenic 45.0%
CACAAGAAAGTCCACGAACA+GGG + chr6_1:96976965-96976984 None:intergenic 45.0%
ACACAAGAAAGTCCACGAAC+AGG + chr6_1:96976966-96976985 None:intergenic 45.0%
ACTGAGAAAGTCCACGAACA+TGG + chr6_1:96977002-96977021 None:intergenic 45.0%
CATTTCTTCCTCTTGCTCCT+TGG + chr6_1:96977792-96977811 None:intergenic 45.0%
GGTTCCAAAGTTGCAGAGAA+TGG - chr6_1:96977860-96977879 Msa0900770:intron 45.0%
TTGCACCTTGTGTTGGAAAC+TGG - chr6_1:96977885-96977904 Msa0900770:intron 45.0%
CTACACCAGTTTCCAACACA+AGG + chr6_1:96977893-96977912 None:intergenic 45.0%
CTGTCTGCTAGGAATGTTGT+TGG - chr6_1:96977921-96977940 Msa0900770:intron 45.0%
GTCTGCTAGGAATGTTGTTG+GGG - chr6_1:96977923-96977942 Msa0900770:intron 45.0%
TGCATTATGTGCAGGTAGCT+TGG - chr6_1:96977961-96977980 Msa0900770:five_prime_UTR 45.0%
! GAAGAGAAGTGAGACGGTTT+TGG + chr6_1:96975872-96975891 None:intergenic 45.0%
TGAAAGGGTTTCCTTCGTGA+GGG + chr6_1:96975905-96975924 None:intergenic 45.0%
ATGAAAGGGTTTCCTTCGTG+AGG + chr6_1:96975906-96975925 None:intergenic 45.0%
! GGACTTTGCCAAAGCATCTT+TGG + chr6_1:96976376-96976395 None:intergenic 45.0%
CACAAGAAAGTCCACGAACA+GGG + chr6_1:96976965-96976984 None:intergenic 45.0%
ACACAAGAAAGTCCACGAAC+AGG + chr6_1:96976966-96976985 None:intergenic 45.0%
ACTGAGAAAGTCCACGAACA+TGG + chr6_1:96977002-96977021 None:intergenic 45.0%
CATTTCTTCCTCTTGCTCCT+TGG + chr6_1:96977792-96977811 None:intergenic 45.0%
GGTTCCAAAGTTGCAGAGAA+TGG - chr6_1:96977860-96977879 Msa0900770:intron 45.0%
TTGCACCTTGTGTTGGAAAC+TGG - chr6_1:96977885-96977904 Msa0900770:intron 45.0%
CTACACCAGTTTCCAACACA+AGG + chr6_1:96977893-96977912 None:intergenic 45.0%
CTGTCTGCTAGGAATGTTGT+TGG - chr6_1:96977921-96977940 Msa0900770:intron 45.0%
GTCTGCTAGGAATGTTGTTG+GGG - chr6_1:96977923-96977942 Msa0900770:intron 45.0%
TGCATTATGTGCAGGTAGCT+TGG - chr6_1:96977961-96977980 Msa0900770:five_prime_UTR 45.0%
GAAAGGGTTTCCTTCGTGAG+GGG + chr6_1:96975904-96975923 None:intergenic 50.0%
CGCATTCAAAACCCTGTTCG+TGG - chr6_1:96976951-96976970 Msa0900770:five_prime_UTR 50.0%
TAACAGGACCAAGGAGCAAG+AGG - chr6_1:96977781-96977800 Msa0900770:intron 50.0%
! GTAGTGGGAAGAGGTCTTCT+TGG - chr6_1:96977819-96977838 Msa0900770:five_prime_UTR 50.0%
GAAAGGGTTTCCTTCGTGAG+GGG + chr6_1:96975904-96975923 None:intergenic 50.0%
CGCATTCAAAACCCTGTTCG+TGG - chr6_1:96976951-96976970 Msa0900770:five_prime_UTR 50.0%
TAACAGGACCAAGGAGCAAG+AGG - chr6_1:96977781-96977800 Msa0900770:intron 50.0%
! GTAGTGGGAAGAGGTCTTCT+TGG - chr6_1:96977819-96977838 Msa0900770:five_prime_UTR 50.0%
CGTGAAATCTCCCCTCACGA+AGG - chr6_1:96975891-96975910 Msa0900770:three_prime_UTR 55.0%
AGGGCGATGGAGCGATGAAA+GGG + chr6_1:96975920-96975939 None:intergenic 55.0%
CGTGAAATCTCCCCTCACGA+AGG - chr6_1:96975891-96975910 Msa0900770:three_prime_UTR 55.0%
AGGGCGATGGAGCGATGAAA+GGG + chr6_1:96975920-96975939 None:intergenic 55.0%
GAGGGCGATGGAGCGATGAA+AGG + chr6_1:96975921-96975940 None:intergenic 60.0%
CTTTGTGAGGGAGAGGGCGA+TGG + chr6_1:96975933-96975952 None:intergenic 60.0%
CATCGCCCTCTCCCTCACAA+AGG - chr6_1:96975931-96975950 Msa0900770:three_prime_UTR 60.0%
GAGGGCGATGGAGCGATGAA+AGG + chr6_1:96975921-96975940 None:intergenic 60.0%
CTTTGTGAGGGAGAGGGCGA+TGG + chr6_1:96975933-96975952 None:intergenic 60.0%
CATCGCCCTCTCCCTCACAA+AGG - chr6_1:96975931-96975950 Msa0900770:three_prime_UTR 60.0%
Chromosome Type Strat End Strand Name
chr6_1 gene 96975793 96978120 96975793 ID=Msa0900770;Name=Msa0900770
chr6_1 mRNA 96975793 96978120 96975793 ID=Msa0900770-mRNA-1;Parent=Msa0900770;Name=Msa0900770-mRNA-1;_AED=0.21;_eAED=0.21;_QI=398|0.66|1|1|0.33|0.25|4|189|74
chr6_1 exon 96975793 96976126 96975793 ID=Msa0900770-mRNA-1:exon:16890;Parent=Msa0900770-mRNA-1
chr6_1 exon 96976833 96977055 96976833 ID=Msa0900770-mRNA-1:exon:16889;Parent=Msa0900770-mRNA-1
chr6_1 exon 96977782 96977876 96977782 ID=Msa0900770-mRNA-1:exon:16888;Parent=Msa0900770-mRNA-1
chr6_1 exon 96977961 96978120 96977961 ID=Msa0900770-mRNA-1:exon:16887;Parent=Msa0900770-mRNA-1
chr6_1 five_prime_UTR 96977961 96978120 96977961 ID=Msa0900770-mRNA-1:five_prime_utr;Parent=Msa0900770-mRNA-1
chr6_1 five_prime_UTR 96977782 96977876 96977782 ID=Msa0900770-mRNA-1:five_prime_utr;Parent=Msa0900770-mRNA-1
chr6_1 five_prime_UTR 96976913 96977055 96976913 ID=Msa0900770-mRNA-1:five_prime_utr;Parent=Msa0900770-mRNA-1
chr6_1 CDS 96976833 96976912 96976833 ID=Msa0900770-mRNA-1:cds;Parent=Msa0900770-mRNA-1
chr6_1 CDS 96975982 96976126 96975982 ID=Msa0900770-mRNA-1:cds;Parent=Msa0900770-mRNA-1
chr6_1 three_prime_UTR 96975793 96975981 96975793 ID=Msa0900770-mRNA-1:three_prime_utr;Parent=Msa0900770-mRNA-1
Gene Sequence

>Msa0900770

ATGTTCGTGGACTTTCTCAGTAGTCATCTCTCTAAAGTTAGTAAGGATAAATTTTTGGATATTGGTTCTGAGTATGAAAGGACCAAGGAGCAAGAGGAAGAAATGATCTTAAGTAGTGGGAAGAGGTCTTCTTGGAAGATTGCAGAGAAAGAAGGTTCCAAAGTTGCAGAGAATGGAATTGCACCTTGTGTTGGAAACTGGTGTAGCAACAATACTGTCTGCTAG
Protein sequence

>Msa0900770

MFVDFLSSHLSKVSKDKFLDIGSEYERTKEQEEEMILSSGKRSSWKIAEKEGSKVAENGIAPCVGNWCSNNTVC*