AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0964580

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0964580 MtrunA17_Chr6g0485021 96.127 284 10 1 1 283 1 284 0.0 561
Msa0964580 MtrunA17_Chr3g0093821 61.111 270 104 1 9 278 1 269 6.61e-124 354
Msa0964580 MtrunA17_Chr1g0152551 44.788 259 140 3 20 277 10 266 3.91e-80 243
Msa0964580 MtrunA17_Chr5g0401521 40.945 254 147 3 21 273 9 260 5.93e-67 211
Msa0964580 MtrunA17_Chr4g0053741 40.796 201 118 1 74 273 9 209 1.67e-51 168
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0964580 AT3G24420.1 55.056 267 118 2 14 279 8 273 2.39e-109 318
Msa0964580 AT3G03990.1 43.284 268 149 3 10 277 1 265 2.40e-79 241
Msa0964580 AT4G37470.1 39.370 254 151 3 21 273 9 260 1.73e-66 208

Find 68 sgRNAs with CRISPR-Local

Find 237 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTTTCTCTTTCTTTAATTA+TGG 0.173279 6_3:+91361445 Msa0964580:exon
TAGAATCCAACTATGAAAAT+TGG 0.176582 6_3:+91364852 Msa0964580:CDS
AGATGACTTGATCAGTTTAT+TGG 0.223698 6_3:+91361727 Msa0964580:CDS
TACATGGAGAAGAAAATTAA+AGG 0.264049 6_3:+91365091 Msa0964580:CDS
TTCACAGATGGTTCATCATT+TGG 0.295535 6_3:-91364905 None:intergenic
TTGGAGGTAATTGACACTTT+TGG 0.320317 6_3:+91365124 Msa0964580:CDS
TCAGCTTGTTGATGTGCTTA+AGG 0.325189 6_3:+91365174 Msa0964580:CDS
TTCAAGAGGCTTATTCTTGT+TGG 0.325449 6_3:+91361839 Msa0964580:CDS
ATGGAACAGACCAATCAATA+TGG 0.335818 6_3:+91361576 Msa0964580:CDS
ATGTAAAGAGCTACACTATA+TGG 0.336358 6_3:-91365073 None:intergenic
TCTTTAATAGCACCAGAAAA+AGG 0.340554 6_3:-91361656 None:intergenic
ATAGAGTTGTTCTATTTGAT+TGG 0.343427 6_3:+91361633 Msa0964580:CDS
CTTAAAGTTGTTAACTTTGT+TGG 0.343903 6_3:+91361761 Msa0964580:CDS
AACATGTTTGGTTTGGTGTT+TGG 0.345702 6_3:-91361470 None:intergenic
TTTGTTGGTCATTCCATGTC+TGG 0.352708 6_3:+91361776 Msa0964580:CDS
CAGCTTGTTGATGTGCTTAA+GGG 0.360603 6_3:+91365175 Msa0964580:CDS
TTATGGTGTTCTAACATGTT+TGG 0.393064 6_3:-91361482 None:intergenic
GATCAGTTTATTGGATCAAA+TGG 0.398901 6_3:+91361736 Msa0964580:CDS
CATTCCATGTCTGGTATGAT+TGG 0.407416 6_3:+91361785 Msa0964580:CDS
AGAATCCAACTATGAAAATT+GGG 0.411548 6_3:+91364853 Msa0964580:CDS
GAAGAGTTGAAAGATTGTTA+TGG 0.425115 6_3:-91361499 None:intergenic
ATTTGGATCCACTACATTTG+TGG 0.429468 6_3:-91364888 None:intergenic
TGTGGTGTTAATTAGATTTG+AGG 0.430458 6_3:+91365540 Msa0964580:three_prime_UTR
TAACCACATATGTTGATGTT+AGG 0.432497 6_3:-91365410 None:intergenic
ACATGGAGAAGAAAATTAAA+GGG 0.433639 6_3:+91365092 Msa0964580:CDS
GTGTTCTAACATGTTTGGTT+TGG 0.447393 6_3:-91361477 None:intergenic
CTTCTGAATGCTAGAATCCT+AGG 0.449257 6_3:+91361518 Msa0964580:CDS
AATGCTAGAATCCTAGGAAC+AGG 0.457236 6_3:+91361524 Msa0964580:CDS
ACTAAACATAGCTAAGTTCA+AGG 0.458492 6_3:-91365220 None:intergenic
AGTGTTGATCAATTAGTGTT+TGG 0.461050 6_3:+91365445 Msa0964580:three_prime_UTR
TAGTTTCTGTTCCTGTTCCT+AGG 0.461589 6_3:-91361535 None:intergenic
TCTAAAGAAGAATACTTTGA+AGG 0.467629 6_3:-91361695 None:intergenic
TATAAATACAGATGACTATG+AGG 0.480351 6_3:+91364787 Msa0964580:CDS
TTATTATTAAATAAAAGTGT+TGG 0.486633 6_3:-91361356 None:intergenic
AATTAAAGGGAAAGTTACAT+TGG 0.494880 6_3:+91365105 Msa0964580:CDS
TTCTTGTTGGTGCTTCCCCA+AGG 0.507570 6_3:+91361852 Msa0964580:CDS
GCACCGACACATCGAATTGA+AGG 0.508092 6_3:+91365361 Msa0964580:three_prime_UTR
TTATGTGTAGTATAGTCCTA+TGG 0.509181 6_3:+91365311 Msa0964580:three_prime_UTR
TTATTTCTCCACAAATGTAG+TGG 0.511610 6_3:+91364880 Msa0964580:CDS
ATATAGTGTAGCTCTTTACA+TGG 0.517253 6_3:+91365075 Msa0964580:CDS
GTAAAATTAAATGTGCCAAA+AGG 0.520600 6_3:-91365275 None:intergenic
TCTCTGAATTTGTTCACAGA+TGG 0.525803 6_3:-91364917 None:intergenic
TGGAACAGACCAATCAATAT+GGG 0.528463 6_3:+91361577 Msa0964580:CDS
TAAAGGGAAAGTTACATTGG+AGG 0.531177 6_3:+91365108 Msa0964580:CDS
CTAAAGAAGAATACTTTGAA+GGG 0.533094 6_3:-91361694 None:intergenic
AGCACATCAACAAGCTGAAG+TGG 0.536506 6_3:-91365169 None:intergenic
GAGGTTTGAATAATTGTGCA+TGG 0.540705 6_3:-91365034 None:intergenic
AAATGAATGAAGTGGAAGAG+AGG 0.548449 6_3:-91361407 None:intergenic
GTCACCGCAAAACACTGTCT+TGG 0.557039 6_3:-91364981 None:intergenic
ATAAGCCTCTTGAAAAGTTG+AGG 0.571930 6_3:-91361830 None:intergenic
ATGCCTAACATCAACATATG+TGG 0.586348 6_3:+91365407 Msa0964580:three_prime_UTR
GCAAAACACTGTCTTGGCTA+AGG 0.590734 6_3:-91364975 None:intergenic
AGATTCATATCCACCTAATG+TGG 0.591947 6_3:-91365598 None:intergenic
ACAACCAATCATACCAGACA+TGG 0.594195 6_3:-91361789 None:intergenic
ACTGTCTTGGCTAAGGACAA+TGG 0.594937 6_3:-91364968 None:intergenic
TCGCCTTCAATTCGATGTGT+CGG 0.600565 6_3:-91365364 None:intergenic
GTGGATATGAATCTTAACAT+TGG 0.601425 6_3:+91365607 Msa0964580:three_prime_UTR
CTAAACATAGCTAAGTTCAA+GGG 0.604175 6_3:-91365219 None:intergenic
CAATATAACTAAGAAACCAT+AGG 0.606735 6_3:-91365327 None:intergenic
ACTAGAAACAATAGAATGTG+TGG 0.609328 6_3:-91361382 None:intergenic
TGAAGTGGAGCAGTTAGTTG+AGG 0.612568 6_3:-91365154 None:intergenic
ATAAATACAGATGACTATGA+GGG 0.625313 6_3:+91364788 Msa0964580:CDS
TATTCAAACCTCTAGTGACA+TGG 0.628354 6_3:+91365045 Msa0964580:CDS
TTGAGGAAATGACAGTGTTG+TGG 0.648866 6_3:+91365557 Msa0964580:three_prime_UTR
TGGAACAACCATGTCACTAG+AGG 0.653480 6_3:-91365053 None:intergenic
AATACAGATGACTATGAGGG+AGG 0.668351 6_3:+91364791 Msa0964580:CDS
ATGAAGCATTCATACAAACA+TGG 0.681879 6_3:-91365471 None:intergenic
ATGAATGCTTCATAGATGTG+TGG 0.740144 6_3:+91365481 Msa0964580:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTAATTTAAAAAAAAAT+TGG + chr6_3:91361957-91361976 Msa0964580:intron 0.0%
!!! CATAAAATAGTTTAAATTTT+AGG + chr6_3:91362501-91362520 Msa0964580:intron 10.0%
!! TAGTAATTAATATTCTTATA+GGG - chr6_3:91362571-91362590 None:intergenic 10.0%
!! ATAGTAATTAATATTCTTAT+AGG - chr6_3:91362572-91362591 None:intergenic 10.0%
!!! AAAAGTCATAAATTTTTTTT+TGG + chr6_3:91362736-91362755 Msa0964580:intron 10.0%
!! TAAAGAATAAAATAAGTATA+TGG + chr6_3:91363623-91363642 Msa0964580:intron 10.0%
!!! ACATTATATACTTTTTTTAT+CGG - chr6_3:91363781-91363800 None:intergenic 10.0%
!!! TAGTTTTTTTCAGTAAAATA+AGG - chr6_3:91361614-91361633 None:intergenic 15.0%
!!! AATTTAGAATTGAAATGAAA+TGG + chr6_3:91362440-91362459 Msa0964580:intron 15.0%
!! AATTTAGATCTTCAAATTAT+AGG + chr6_3:91362775-91362794 Msa0964580:intron 15.0%
!!! CTCTATATTTTATTTTTTGT+CGG - chr6_3:91363926-91363945 None:intergenic 15.0%
!! AATAGATAATTATAAACATG+TGG - chr6_3:91364137-91364156 None:intergenic 15.0%
!!! TTGTGTGTATTTTTTTTTTT+TGG + chr6_3:91364555-91364574 Msa0964580:intron 15.0%
!! TATGTTAAAATAAAATGATG+TGG + chr6_3:91364636-91364655 Msa0964580:intron 15.0%
!! ATTTAAAAGATAAACATCTT+AGG - chr6_3:91364698-91364717 None:intergenic 15.0%
!! TCTTTCTCTTTCTTTAATTA+TGG + chr6_3:91361445-91361464 Msa0964580:exon 20.0%
!! TATATAACAAATATACCTTG+GGG - chr6_3:91361870-91361889 None:intergenic 20.0%
!! GTATATAACAAATATACCTT+GGG - chr6_3:91361871-91361890 None:intergenic 20.0%
!! AGTATATAACAAATATACCT+TGG - chr6_3:91361872-91361891 None:intergenic 20.0%
!! ACAATAACTTTACATTTCAA+AGG - chr6_3:91362219-91362238 None:intergenic 20.0%
!!! ATGTAAAGTTATTGTTTTCT+AGG + chr6_3:91362224-91362243 Msa0964580:intron 20.0%
!! AAATGACAAATGTTAAGAAA+GGG - chr6_3:91362361-91362380 None:intergenic 20.0%
!! AAAATGACAAATGTTAAGAA+AGG - chr6_3:91362362-91362381 None:intergenic 20.0%
!!! AACTATTTTATGATACTACA+AGG - chr6_3:91362493-91362512 None:intergenic 20.0%
!!! TAGTTTAAATTTTAGGGAAT+TGG + chr6_3:91362508-91362527 Msa0964580:intron 20.0%
!! AATTAATATTCTTATAGGGA+TGG - chr6_3:91362567-91362586 None:intergenic 20.0%
!! TAATTACTATATAGATGTGA+TGG + chr6_3:91362582-91362601 Msa0964580:intron 20.0%
!! TAGTAAAAAGGAAAATACTT+TGG - chr6_3:91362691-91362710 None:intergenic 20.0%
!! AAACTAGAGATTTAGTAAAA+AGG - chr6_3:91362703-91362722 None:intergenic 20.0%
!!! GTCATAAATTTTTTTTTGGA+AGG + chr6_3:91362740-91362759 Msa0964580:intron 20.0%
!!! CACTGAAATATTTTTTTGAA+AGG + chr6_3:91362874-91362893 Msa0964580:intron 20.0%
!!! TTTTAGTGGCTAAATTTTTA+AGG + chr6_3:91363007-91363026 Msa0964580:intron 20.0%
!! TTAAGGTTTAAATGTGTAAT+TGG + chr6_3:91363024-91363043 Msa0964580:intron 20.0%
!! CTTTGTAAAAATATTGGTAT+TGG + chr6_3:91363176-91363195 Msa0964580:intron 20.0%
!!! CTTTGAAAAAATATTGGTAT+TGG - chr6_3:91363259-91363278 None:intergenic 20.0%
!! CTTTGTAAAAATATTGGTAT+TGG - chr6_3:91363176-91363195 None:intergenic 20.0%
!!! AATTGGTTTAAATGTGTAAT+TGG - chr6_3:91363441-91363460 None:intergenic 20.0%
!! AACACAACTCTATTAAAAAT+TGG - chr6_3:91363458-91363477 None:intergenic 20.0%
!! AGAATAAAATAAGTATATGG+TGG + chr6_3:91363626-91363645 Msa0964580:intron 20.0%
!!! TATTTTTATTTGTTAGCTAG+AGG - chr6_3:91363960-91363979 None:intergenic 20.0%
!! ATAATTATAGTGATGTAAGT+GGG - chr6_3:91364004-91364023 None:intergenic 20.0%
!! AATAATTATAGTGATGTAAG+TGG - chr6_3:91364005-91364024 None:intergenic 20.0%
!!! AACTTTTGATAAAATGTGAT+TGG + chr6_3:91364033-91364052 Msa0964580:intron 20.0%
!!! AAGTATGTATACTCATTTTT+AGG + chr6_3:91364373-91364392 Msa0964580:intron 20.0%
!! CACAATATAAGATAATTCAA+CGG - chr6_3:91364495-91364514 None:intergenic 20.0%
!!! GTGTATTTTTTTTTTTTGGA+AGG + chr6_3:91364559-91364578 Msa0964580:intron 20.0%
! AAGAAGAAAAATGAATGAAG+TGG - chr6_3:91361418-91361437 None:intergenic 25.0%
! ATAGAGTTGTTCTATTTGAT+TGG + chr6_3:91361633-91361652 Msa0964580:CDS 25.0%
! CTAAAGAAGAATACTTTGAA+GGG - chr6_3:91361697-91361716 None:intergenic 25.0%
! TCTAAAGAAGAATACTTTGA+AGG - chr6_3:91361698-91361717 None:intergenic 25.0%
! CTTAAAGTTGTTAACTTTGT+TGG + chr6_3:91361761-91361780 Msa0964580:CDS 25.0%
!!! CTTTGTTTGTTATTGTTACT+TGG + chr6_3:91362117-91362136 Msa0964580:intron 25.0%
! GTAATAGACTATATGAGATA+AGG + chr6_3:91362645-91362664 Msa0964580:intron 25.0%
! AAAAGGAAAATACTTTGGTT+GGG - chr6_3:91362686-91362705 None:intergenic 25.0%
! AAAAAGGAAAATACTTTGGT+TGG - chr6_3:91362687-91362706 None:intergenic 25.0%
! AAATCTTAAGAACAGATACT+TGG - chr6_3:91362990-91363009 None:intergenic 25.0%
!! ATCTGTTCTTAAGATTTTAG+TGG + chr6_3:91362993-91363012 Msa0964580:intron 25.0%
!!! ACTTTTTTTTTGTTTGGCAT+TGG + chr6_3:91363085-91363104 Msa0964580:intron 25.0%
!!! CAATATTTTTACAAAGTGCA+GGG - chr6_3:91363294-91363313 None:intergenic 25.0%
! CTTTGTAAAAATATTGGCAT+TGG + chr6_3:91363116-91363135 Msa0964580:intron 25.0%
!!! CAATATTTTTACAAAGTGCA+GGG - chr6_3:91363294-91363313 None:intergenic 25.0%
!! CTTTGGAAAAATATTGGTAT+TGG + chr6_3:91363207-91363226 Msa0964580:intron 25.0%
!!! CAATATTTTTTCAAAGTGCA+GGG + chr6_3:91363263-91363282 Msa0964580:intron 25.0%
!!! CAATATTTTTACAAAGTGCA+GGG + chr6_3:91363294-91363313 Msa0964580:intron 25.0%
! CTTTGTAAAAATATTGGCAT+TGG - chr6_3:91363116-91363135 None:intergenic 25.0%
!!! CAATATTTTTACAAAGTGCA+GGG + chr6_3:91363294-91363313 Msa0964580:intron 25.0%
!! TATGTTGTGATTCCTTTTTA+AGG + chr6_3:91363563-91363582 Msa0964580:intron 25.0%
!!! TTTTTAAGGTGAATTTGATG+CGG + chr6_3:91363577-91363596 Msa0964580:intron 25.0%
! TAATTATAGTGATGTAAGTG+GGG - chr6_3:91364003-91364022 None:intergenic 25.0%
! ATTTATACAAGGAAATGTCT+TGG - chr6_3:91364099-91364118 None:intergenic 25.0%
! AATCTCTTTGGATTTATACA+AGG - chr6_3:91364110-91364129 None:intergenic 25.0%
!!! ATCGGTTTTAATAACATCAA+AGG - chr6_3:91364281-91364300 None:intergenic 25.0%
! GTTAAAATAAAATGATGTGG+TGG + chr6_3:91364639-91364658 Msa0964580:intron 25.0%
! TATAAATACAGATGACTATG+AGG + chr6_3:91364787-91364806 Msa0964580:CDS 25.0%
! ATAAATACAGATGACTATGA+GGG + chr6_3:91364788-91364807 Msa0964580:CDS 25.0%
! TAGAATCCAACTATGAAAAT+TGG + chr6_3:91364852-91364871 Msa0964580:CDS 25.0%
! AGAATCCAACTATGAAAATT+GGG + chr6_3:91364853-91364872 Msa0964580:CDS 25.0%
! TACATGGAGAAGAAAATTAA+AGG + chr6_3:91365091-91365110 Msa0964580:CDS 25.0%
! ACATGGAGAAGAAAATTAAA+GGG + chr6_3:91365092-91365111 Msa0964580:CDS 25.0%
! AATTAAAGGGAAAGTTACAT+TGG + chr6_3:91365105-91365124 Msa0964580:CDS 25.0%
!! GTTTTCAATTTCATTCCTTT+TGG + chr6_3:91365260-91365279 Msa0964580:three_prime_UTR 25.0%
! GTAAAATTAAATGTGCCAAA+AGG - chr6_3:91365278-91365297 None:intergenic 25.0%
! CAATATAACTAAGAAACCAT+AGG - chr6_3:91365330-91365349 None:intergenic 25.0%
!!! TGTTTGTAGTTTTGTATTTG+TGG + chr6_3:91365522-91365541 Msa0964580:three_prime_UTR 25.0%
ACTAGAAACAATAGAATGTG+TGG - chr6_3:91361385-91361404 None:intergenic 30.0%
TTATGGTGTTCTAACATGTT+TGG - chr6_3:91361485-91361504 None:intergenic 30.0%
GAAGAGTTGAAAGATTGTTA+TGG - chr6_3:91361502-91361521 None:intergenic 30.0%
! GAAACTATAGTTTTTGCTCA+TGG + chr6_3:91361551-91361570 Msa0964580:CDS 30.0%
! ATAGTTTTTGCTCATGGTTA+TGG + chr6_3:91361557-91361576 Msa0964580:CDS 30.0%
! TGATTTTGTCCCATATTGAT+TGG - chr6_3:91361589-91361608 None:intergenic 30.0%
TCTTTAATAGCACCAGAAAA+AGG - chr6_3:91361659-91361678 None:intergenic 30.0%
AGATGACTTGATCAGTTTAT+TGG + chr6_3:91361727-91361746 Msa0964580:CDS 30.0%
GATCAGTTTATTGGATCAAA+TGG + chr6_3:91361736-91361755 Msa0964580:CDS 30.0%
! GAAAAGTTGAGGTCTTTTAA+TGG - chr6_3:91361822-91361841 None:intergenic 30.0%
CAAACAAAGAACATAGTTAC+AGG - chr6_3:91362106-91362125 None:intergenic 30.0%
TTCAAAGGATAAAGAAGAAG+AGG - chr6_3:91362204-91362223 None:intergenic 30.0%
CTCATATAGTCTATTACAAG+TGG - chr6_3:91362642-91362661 None:intergenic 30.0%
AAAGGAAAATACTTTGGTTG+GGG - chr6_3:91362685-91362704 None:intergenic 30.0%
! CCAAACAAAAAAAAAGTGCA+GGG - chr6_3:91363082-91363101 None:intergenic 30.0%
!! CCAATATTTTTACAAAGTGC+AGG - chr6_3:91363293-91363312 None:intergenic 30.0%
CCTGCACTTTGTAAAAATAT+TGG + chr6_3:91363110-91363129 Msa0964580:intron 30.0%
!!! CACTTTTTTTTGTTTGGCAT+TGG + chr6_3:91363145-91363164 Msa0964580:intron 30.0%
!! CCAATATTTTTACAAAGTGC+AGG - chr6_3:91363293-91363312 None:intergenic 30.0%
CCTGCACTTTGTAAAAATAT+TGG + chr6_3:91363110-91363129 Msa0964580:intron 30.0%
! CAATATTTTTCCAAAGTGCA+GGG - chr6_3:91363203-91363222 None:intergenic 30.0%
! CCTGCACTTTGAAAAAATAT+TGG - chr6_3:91363265-91363284 None:intergenic 30.0%
!!! CCAATATTTTTTCAAAGTGC+AGG + chr6_3:91363262-91363281 Msa0964580:intron 30.0%
CCTGCACTTTGTAAAAATAT+TGG - chr6_3:91363110-91363129 None:intergenic 30.0%
!! CCAATATTTTTACAAAGTGC+AGG + chr6_3:91363293-91363312 Msa0964580:intron 30.0%
!!! CACTTTTTTTTGTTTGGCAT+TGG - chr6_3:91363145-91363164 None:intergenic 30.0%
CCTGCACTTTGTAAAAATAT+TGG - chr6_3:91363110-91363129 None:intergenic 30.0%
!! CCAATATTTTTACAAAGTGC+AGG + chr6_3:91363293-91363312 Msa0964580:intron 30.0%
!!! CACTTTTTTTTGTTTGGCAT+TGG - chr6_3:91363145-91363164 None:intergenic 30.0%
!! CTTTTAACAGATTTTGGCAT+TGG - chr6_3:91363410-91363429 None:intergenic 30.0%
! CAAAATCTGTTAAAAGTGCA+GGG + chr6_3:91363414-91363433 Msa0964580:intron 30.0%
AATGAACCAACCAAAACATA+TGG + chr6_3:91363488-91363507 Msa0964580:intron 30.0%
GCATCAAATTCACCTTAAAA+AGG - chr6_3:91363578-91363597 None:intergenic 30.0%
TAGATAATGCTAAGATCGTT+AGG + chr6_3:91363667-91363686 Msa0964580:intron 30.0%
! TTGGTGGTTCAAATTTCAAA+AGG - chr6_3:91364068-91364087 None:intergenic 30.0%
! CCTAAAAGGTTCTTTTTGTT+GGG - chr6_3:91364168-91364187 None:intergenic 30.0%
! ACCTAAAAGGTTCTTTTTGT+TGG - chr6_3:91364169-91364188 None:intergenic 30.0%
! ACAATAGAGATGTCAACTTT+TGG + chr6_3:91364243-91364262 Msa0964580:intron 30.0%
TGTGTCTATCTTAGAGAAAT+CGG - chr6_3:91364299-91364318 None:intergenic 30.0%
! AGAAACCCAATTTTCATAGT+TGG - chr6_3:91364861-91364880 None:intergenic 30.0%
! TTATTTCTCCACAAATGTAG+TGG + chr6_3:91364880-91364899 Msa0964580:CDS 30.0%
ATGTAAAGAGCTACACTATA+TGG - chr6_3:91365076-91365095 None:intergenic 30.0%
ATATAGTGTAGCTCTTTACA+TGG + chr6_3:91365075-91365094 Msa0964580:CDS 30.0%
CTAAACATAGCTAAGTTCAA+GGG - chr6_3:91365222-91365241 None:intergenic 30.0%
ACTAAACATAGCTAAGTTCA+AGG - chr6_3:91365223-91365242 None:intergenic 30.0%
TTATGTGTAGTATAGTCCTA+TGG + chr6_3:91365311-91365330 Msa0964580:three_prime_UTR 30.0%
TAACCACATATGTTGATGTT+AGG - chr6_3:91365413-91365432 None:intergenic 30.0%
!! AGTGTTGATCAATTAGTGTT+TGG + chr6_3:91365445-91365464 Msa0964580:three_prime_UTR 30.0%
! ATGAAGCATTCATACAAACA+TGG - chr6_3:91365474-91365493 None:intergenic 30.0%
! TGTGGTGTTAATTAGATTTG+AGG + chr6_3:91365540-91365559 Msa0964580:three_prime_UTR 30.0%
CCTAATGTGGAAAAAAGTTA+TGG - chr6_3:91365588-91365607 None:intergenic 30.0%
!!! CCATAACTTTTTTCCACATT+AGG + chr6_3:91365585-91365604 Msa0964580:three_prime_UTR 30.0%
!!! TAACTTTTTTCCACATTAGG+TGG + chr6_3:91365588-91365607 Msa0964580:three_prime_UTR 30.0%
GTGGATATGAATCTTAACAT+TGG + chr6_3:91365607-91365626 Msa0964580:three_prime_UTR 30.0%
AAATGAATGAAGTGGAAGAG+AGG - chr6_3:91361410-91361429 None:intergenic 35.0%
!! AACATGTTTGGTTTGGTGTT+TGG - chr6_3:91361473-91361492 None:intergenic 35.0%
GTGTTCTAACATGTTTGGTT+TGG - chr6_3:91361480-91361499 None:intergenic 35.0%
ATGGAACAGACCAATCAATA+TGG + chr6_3:91361576-91361595 Msa0964580:CDS 35.0%
TGGAACAGACCAATCAATAT+GGG + chr6_3:91361577-91361596 Msa0964580:CDS 35.0%
!!! CTATTTGATTGGCCTTTTTC+TGG + chr6_3:91361644-91361663 Msa0964580:CDS 35.0%
!! AAAGACCTCAACTTTTCAAG+AGG + chr6_3:91361825-91361844 Msa0964580:CDS 35.0%
ATAAGCCTCTTGAAAAGTTG+AGG - chr6_3:91361833-91361852 None:intergenic 35.0%
TTCAAGAGGCTTATTCTTGT+TGG + chr6_3:91361839-91361858 Msa0964580:CDS 35.0%
ACAAAGAACATAGTTACAGG+AGG - chr6_3:91362103-91362122 None:intergenic 35.0%
GAGAACAACAAAGGGAAAAA+AGG - chr6_3:91362320-91362339 None:intergenic 35.0%
TGTTTGAGGAGAACAACAAA+GGG - chr6_3:91362328-91362347 None:intergenic 35.0%
ATGTTTGAGGAGAACAACAA+AGG - chr6_3:91362329-91362348 None:intergenic 35.0%
AGGGAATATATGCATGTTTG+AGG - chr6_3:91362342-91362361 None:intergenic 35.0%
! CAAAATCTGTTGAAAGTGCA+GGG - chr6_3:91363051-91363070 None:intergenic 35.0%
!! CTTTCAACAGATTTTGGCAT+TGG + chr6_3:91363055-91363074 Msa0964580:intron 35.0%
!!! CCCTGCACTTTTTTTTTGTT+TGG + chr6_3:91363079-91363098 Msa0964580:intron 35.0%
! GCCAAACAAAAAAAAAGTGC+AGG - chr6_3:91363083-91363102 None:intergenic 35.0%
!!! TCCCTGCACTTTTTTTTGTT+TGG + chr6_3:91363139-91363158 Msa0964580:intron 35.0%
! GCCAAACAAAAAAAAGTGCA+GGG - chr6_3:91363323-91363342 None:intergenic 35.0%
! TGCCAAACAAAAAAAAGTGC+AGG - chr6_3:91363322-91363341 None:intergenic 35.0%
! CCAATATTTTTCCAAAGTGC+AGG - chr6_3:91363204-91363223 None:intergenic 35.0%
! CCTGCACTTTGGAAAAATAT+TGG + chr6_3:91363201-91363220 Msa0964580:intron 35.0%
! TGCCAAACAAAAAAAAGTGC+AGG + chr6_3:91363322-91363341 Msa0964580:intron 35.0%
!!! TGCCTGCACTTTTTTTTGTT+TGG - chr6_3:91363327-91363346 None:intergenic 35.0%
! TGCCAAACAAAAAAAAGTGC+AGG + chr6_3:91363322-91363341 Msa0964580:intron 35.0%
! GCCAAACAAAAAAAAGTGCA+GGG + chr6_3:91363323-91363342 Msa0964580:intron 35.0%
!!! TCCCTGCACTTTTTTTTGTT+TGG - chr6_3:91363139-91363158 None:intergenic 35.0%
! CCTGCACTTTTAACAGATTT+TGG - chr6_3:91363416-91363435 None:intergenic 35.0%
! CCAAAATCTGTTAAAAGTGC+AGG + chr6_3:91363413-91363432 Msa0964580:intron 35.0%
TTGACAATGCTAAGATCGTT+AGG + chr6_3:91363810-91363829 Msa0964580:intron 35.0%
GTGGTTCAAATTTCAAAAGG+AGG - chr6_3:91364065-91364084 None:intergenic 35.0%
AATTTGAACCACCAATTAGC+CGG + chr6_3:91364073-91364092 Msa0964580:intron 35.0%
ACATGTGGAAGAAATCTCTT+TGG - chr6_3:91364122-91364141 None:intergenic 35.0%
! CCCAACAAAAAGAACCTTTT+AGG + chr6_3:91364165-91364184 Msa0964580:intron 35.0%
ATTTGGATCCACTACATTTG+TGG - chr6_3:91364891-91364910 None:intergenic 35.0%
TTCACAGATGGTTCATCATT+TGG - chr6_3:91364908-91364927 None:intergenic 35.0%
! TCTCTGAATTTGTTCACAGA+TGG - chr6_3:91364920-91364939 None:intergenic 35.0%
GAGGTTTGAATAATTGTGCA+TGG - chr6_3:91365037-91365056 None:intergenic 35.0%
TATTCAAACCTCTAGTGACA+TGG + chr6_3:91365045-91365064 Msa0964580:CDS 35.0%
TAAAGGGAAAGTTACATTGG+AGG + chr6_3:91365108-91365127 Msa0964580:CDS 35.0%
! TTGGAGGTAATTGACACTTT+TGG + chr6_3:91365124-91365143 Msa0964580:CDS 35.0%
!!! ATGTGCTTAAGGGTGTTTTA+GGG + chr6_3:91365185-91365204 Msa0964580:CDS 35.0%
ATGCCTAACATCAACATATG+TGG + chr6_3:91365407-91365426 Msa0964580:three_prime_UTR 35.0%
ATGAATGCTTCATAGATGTG+TGG + chr6_3:91365481-91365500 Msa0964580:three_prime_UTR 35.0%
AGATTCATATCCACCTAATG+TGG - chr6_3:91365601-91365620 None:intergenic 35.0%
CTTCTGAATGCTAGAATCCT+AGG + chr6_3:91361518-91361537 Msa0964580:CDS 40.0%
AATGCTAGAATCCTAGGAAC+AGG + chr6_3:91361524-91361543 Msa0964580:CDS 40.0%
TAGTTTCTGTTCCTGTTCCT+AGG - chr6_3:91361538-91361557 None:intergenic 40.0%
TTTGTTGGTCATTCCATGTC+TGG + chr6_3:91361776-91361795 Msa0964580:CDS 40.0%
CATTCCATGTCTGGTATGAT+TGG + chr6_3:91361785-91361804 Msa0964580:CDS 40.0%
ACAACCAATCATACCAGACA+TGG - chr6_3:91361792-91361811 None:intergenic 40.0%
TTCTTCCCAAAATACTGCCT+AGG + chr6_3:91362537-91362556 Msa0964580:intron 40.0%
! GAAGTCCTAGGCAGTATTTT+GGG - chr6_3:91362545-91362564 None:intergenic 40.0%
TACAAGTGGCCTAGTTCATT+TGG - chr6_3:91362628-91362647 None:intergenic 40.0%
! CCAAAATCTGTTGAAAGTGC+AGG - chr6_3:91363052-91363071 None:intergenic 40.0%
! CCTGCACTTTCAACAGATTT+TGG + chr6_3:91363049-91363068 Msa0964580:intron 40.0%
!!! CTGACACCATATGTTTTGGT+TGG - chr6_3:91363497-91363516 None:intergenic 40.0%
! CCTACTGACACCATATGTTT+TGG - chr6_3:91363501-91363520 None:intergenic 40.0%
! CCAAAACATATGGTGTCAGT+AGG + chr6_3:91363498-91363517 Msa0964580:intron 40.0%
! ACATATGGTGTCAGTAGGAT+TGG + chr6_3:91363503-91363522 Msa0964580:intron 40.0%
! CATATGGTGTCAGTAGGATT+GGG + chr6_3:91363504-91363523 Msa0964580:intron 40.0%
! ATTTGTTAGCTAGAGGCATG+TGG - chr6_3:91363953-91363972 None:intergenic 40.0%
ATACAAGGAAATGTCTTGGC+CGG - chr6_3:91364095-91364114 None:intergenic 40.0%
!! AGAACCTTTTAGGTACAGCT+AGG + chr6_3:91364175-91364194 Msa0964580:intron 40.0%
CTTTCCTAGCTGTACCTAAA+AGG - chr6_3:91364182-91364201 None:intergenic 40.0%
AATACAGATGACTATGAGGG+AGG + chr6_3:91364791-91364810 Msa0964580:CDS 40.0%
! TTAGCCAAGACAGTGTTTTG+CGG + chr6_3:91364977-91364996 Msa0964580:CDS 40.0%
!! TCAGCTTGTTGATGTGCTTA+AGG + chr6_3:91365174-91365193 Msa0964580:CDS 40.0%
!! CAGCTTGTTGATGTGCTTAA+GGG + chr6_3:91365175-91365194 Msa0964580:CDS 40.0%
!!! GATGTGCTTAAGGGTGTTTT+AGG + chr6_3:91365184-91365203 Msa0964580:CDS 40.0%
TTGAGGAAATGACAGTGTTG+TGG + chr6_3:91365557-91365576 Msa0964580:three_prime_UTR 40.0%
! GGAAGTCCTAGGCAGTATTT+TGG - chr6_3:91362546-91362565 None:intergenic 45.0%
CTTATAGGGATGGAAGTCCT+AGG - chr6_3:91362557-91362576 None:intergenic 45.0%
CACCTCATGCCAAATGAACT+AGG + chr6_3:91362616-91362635 Msa0964580:intron 45.0%
CCACGTGGCATGAAATGATT+GGG - chr6_3:91363234-91363253 None:intergenic 45.0%
!! ATATTGGTATTGGTGCCACG+TGG - chr6_3:91363249-91363268 None:intergenic 45.0%
TGTCAGTAGGATTGGGTAAG+AGG + chr6_3:91363511-91363530 Msa0964580:intron 45.0%
GTCAGTAGGATTGGGTAAGA+GGG + chr6_3:91363512-91363531 Msa0964580:intron 45.0%
TCAGTAGGATTGGGTAAGAG+GGG + chr6_3:91363513-91363532 Msa0964580:intron 45.0%
TAAGGTGAATTTGATGCGGC+TGG + chr6_3:91363581-91363600 Msa0964580:intron 45.0%
AAATGTCTTGGCCGGCTAAT+TGG - chr6_3:91364087-91364106 None:intergenic 45.0%
ACTGTCTTGGCTAAGGACAA+TGG - chr6_3:91364971-91364990 None:intergenic 45.0%
GCAAAACACTGTCTTGGCTA+AGG - chr6_3:91364978-91364997 None:intergenic 45.0%
TGGAACAACCATGTCACTAG+AGG - chr6_3:91365056-91365075 None:intergenic 45.0%
TGAAGTGGAGCAGTTAGTTG+AGG - chr6_3:91365157-91365176 None:intergenic 45.0%
AGCACATCAACAAGCTGAAG+TGG - chr6_3:91365172-91365191 None:intergenic 45.0%
TCGCCTTCAATTCGATGTGT+CGG - chr6_3:91365367-91365386 None:intergenic 45.0%
!!! ATAAAATAGTTTAAATTTTA+GGG + chr6_3:91362502-91362521 Msa0964580:intron 5.0%
!!! TTTAAATTTTATTTAATTTC+AGG - chr6_3:91362820-91362839 None:intergenic 5.0%
!!! ATTTTATTCTTTAATTTTTA+TGG - chr6_3:91363616-91363635 None:intergenic 5.0%
! TTCTTGTTGGTGCTTCCCCA+AGG + chr6_3:91361852-91361871 Msa0964580:CDS 50.0%
GGCCTAGTTCATTTGGCATG+AGG - chr6_3:91362621-91362640 None:intergenic 50.0%
!! TGGTATTGGTCCCTGCACTT+TGG + chr6_3:91363190-91363209 Msa0964580:intron 50.0%
CCCAATCATTTCATGCCACG+TGG + chr6_3:91363231-91363250 Msa0964580:intron 50.0%
GCCACGTGGCATGAAATGAT+TGG - chr6_3:91363235-91363254 None:intergenic 50.0%
GTCACCGCAAAACACTGTCT+TGG - chr6_3:91364984-91365003 None:intergenic 50.0%
! GCACCGACACATCGAATTGA+AGG + chr6_3:91365361-91365380 Msa0964580:three_prime_UTR 50.0%
TGTCTTGGCCGGCTAATTGG+TGG - chr6_3:91364084-91364103 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr6_3 gene 91361357 91365660 91361357 ID=Msa0964580;Name=Msa0964580
chr6_3 mRNA 91361357 91365660 91361357 ID=Msa0964580-mRNA-1;Parent=Msa0964580;Name=Msa0964580-mRNA-1;_AED=0.00;_eAED=0.00;_QI=107|1|1|1|1|1|2|435|283
chr6_3 exon 91361357 91361873 91361357 ID=Msa0964580-mRNA-1:exon:16449;Parent=Msa0964580-mRNA-1
chr6_3 exon 91364784 91365660 91364784 ID=Msa0964580-mRNA-1:exon:16450;Parent=Msa0964580-mRNA-1
chr6_3 five_prime_UTR 91361357 91361463 91361357 ID=Msa0964580-mRNA-1:five_prime_utr;Parent=Msa0964580-mRNA-1
chr6_3 CDS 91361464 91361873 91361464 ID=Msa0964580-mRNA-1:cds;Parent=Msa0964580-mRNA-1
chr6_3 CDS 91364784 91365225 91364784 ID=Msa0964580-mRNA-1:cds;Parent=Msa0964580-mRNA-1
chr6_3 three_prime_UTR 91365226 91365660 91365226 ID=Msa0964580-mRNA-1:three_prime_utr;Parent=Msa0964580-mRNA-1
Gene Sequence

>Msa0964580

ATGGAACCAAACACCAAACCAAACATGTTAGAACACCATAACAATCTTTCAACTCTTCTGAATGCTAGAATCCTAGGAACAGGAACAGAAACTATAGTTTTTGCTCATGGTTATGGAACAGACCAATCAATATGGGACAAAATCACACCTTATTTTACTGAAAAAAACTATAGAGTTGTTCTATTTGATTGGCCTTTTTCTGGTGCTATTAAAGATCAAAATCTTTATGACCCTTCAAAGTATTCTTCTTTAGATGCTTTTGCAGATGACTTGATCAGTTTATTGGATCAAATGGAGCTTAAAGTTGTTAACTTTGTTGGTCATTCCATGTCTGGTATGATTGGTTGTTTAGCTTCCATTAAAAGACCTCAACTTTTCAAGAGGCTTATTCTTGTTGGTGCTTCCCCAAGATATATAAATACAGATGACTATGAGGGAGGATTCACAAGCTCAGATATTGAGAATTTATTGAAGAACATAGAATCCAACTATGAAAATTGGGTTTCTTATTTCTCCACAAATGTAGTGGATCCAAATGATGAACCATCTGTGAACAAATTCAGAGAGTGTTTGAAAAAAATGAGAAATGAAGTTCCATTGTCCTTAGCCAAGACAGTGTTTTGCGGTGACTATAGAGATATACTTGAGAAAGTTGAAACTCCATGCACAATTATTCAAACCTCTAGTGACATGGTTGTTCCATATAGTGTAGCTCTTTACATGGAGAAGAAAATTAAAGGGAAAGTTACATTGGAGGTAATTGACACTTTTGGACATTTTCCTCAACTAACTGCTCCACTTCAGCTTGTTGATGTGCTTAAGGGTGTTTTAGGGTTTGATCACTTCCCTTGA
Protein sequence

>Msa0964580

MEPNTKPNMLEHHNNLSTLLNARILGTGTETIVFAHGYGTDQSIWDKITPYFTEKNYRVVLFDWPFSGAIKDQNLYDPSKYSSLDAFADDLISLLDQMELKVVNFVGHSMSGMIGCLASIKRPQLFKRLILVGASPRYINTDDYEGGFTSSDIENLLKNIESNYENWVSYFSTNVVDPNDEPSVNKFRECLKKMRNEVPLSLAKTVFCGDYRDILEKVETPCTIIQTSSDMVVPYSVALYMEKKIKGKVTLEVIDTFGHFPQLTAPLQLVDVLKGVLGFDHFP*