AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0899130

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0899130 MtrunA17_Chr6g0485021 95.070 284 13 1 1 283 1 284 0.0 559
Msa0899130 MtrunA17_Chr3g0093821 61.481 270 103 1 9 278 1 269 6.02e-125 357
Msa0899130 MtrunA17_Chr1g0152551 44.402 259 141 3 20 277 10 266 1.40e-79 241
Msa0899130 MtrunA17_Chr5g0401521 40.551 254 148 3 21 273 9 260 7.21e-67 210
Msa0899130 MtrunA17_Chr4g0053741 40.796 201 118 1 74 273 9 209 5.67e-52 169
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0899130 AT3G24420.1 54.815 270 120 2 11 279 5 273 3.70e-109 317
Msa0899130 AT3G03990.1 42.910 268 150 3 10 277 1 265 3.59e-79 241
Msa0899130 AT4G37470.1 38.976 254 152 3 21 273 9 260 2.04e-66 208

Find 66 sgRNAs with CRISPR-Local

Find 197 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTTTCTCTTTCTTTAATTA+TGG 0.173279 6_1:-93964153 Msa0899130:exon
TAGAATCCAACTATGAAAAT+TGG 0.176582 6_1:-93960983 Msa0899130:CDS
AGATGACTTGATCAGTTTAT+TGG 0.223698 6_1:-93963871 Msa0899130:CDS
TTCACTGATGGTTCATCATT+TGG 0.234984 6_1:+93960930 None:intergenic
TACATGGAGAAGAAAATTAA+AGG 0.264049 6_1:-93960744 Msa0899130:CDS
TTGGAGATAATTGACACTTT+TGG 0.295294 6_1:-93960711 Msa0899130:CDS
TTCAAGAGGCTTATTCTTGT+TGG 0.325449 6_1:-93963759 Msa0899130:CDS
ATGGAACAGACCAATCAATA+TGG 0.335818 6_1:-93964022 Msa0899130:CDS
ATGTAAAGAGCTACACTATA+TGG 0.336358 6_1:+93960762 None:intergenic
TCTTTAATAGCACCAGAAAA+AGG 0.340554 6_1:+93963942 None:intergenic
TCAGCTGGTTGATGTGCTTA+AGG 0.340672 6_1:-93960661 Msa0899130:CDS
ACAAAGTTGTTCTATTTGAT+TGG 0.344498 6_1:-93963965 Msa0899130:CDS
AACATGTTTGGTTTGGTGTT+TGG 0.345702 6_1:+93964128 None:intergenic
ATTTGTGTAGTATAGTTCTA+TGG 0.347130 6_1:-93960526 Msa0899130:three_prime_UTR
CTTAAAGTTGTTAACTTTGT+TGG 0.349814 6_1:-93963837 Msa0899130:CDS
TTTGTTGGTCATTCCATGTC+TGG 0.352708 6_1:-93963822 Msa0899130:CDS
TTATGCTGTTCTAACATGTT+TGG 0.379798 6_1:+93964116 None:intergenic
CAGCTGGTTGATGTGCTTAA+GGG 0.382841 6_1:-93960660 Msa0899130:CDS
CATTCCATGTCTGGTATGAT+TGG 0.407416 6_1:-93963813 Msa0899130:CDS
AGAATCCAACTATGAAAATT+GGG 0.411548 6_1:-93960982 Msa0899130:CDS
GATCAGTTTATTGGATCAAA+TGG 0.413857 6_1:-93963862 Msa0899130:CDS
ATTTGGATCCACTACATTTG+TGG 0.429468 6_1:+93960947 None:intergenic
CTGTTCTAACATGTTTGGTT+TGG 0.431637 6_1:+93964121 None:intergenic
ACATGGAGAAGAAAATTAAA+GGG 0.433639 6_1:-93960743 Msa0899130:CDS
TGGGGTGTTAATTAGATTTG+AGG 0.433751 6_1:-93960296 Msa0899130:three_prime_UTR
TCTAAAGAAGAATACTTTGA+AGG 0.446152 6_1:+93963903 None:intergenic
CTTCTGAATGCTAGAATCCT+AGG 0.449257 6_1:-93964080 Msa0899130:CDS
AATGCTAGAATCCTAGGAAC+AGG 0.457236 6_1:-93964074 Msa0899130:CDS
TAGTTTCTGTTCCTGTTCCT+AGG 0.461589 6_1:+93964063 None:intergenic
ACTAAACATAGCTAAATTCA+AGG 0.462612 6_1:+93960615 None:intergenic
TATAAATACAGATGACTATG+AGG 0.480351 6_1:-93961048 Msa0899130:CDS
AATTAAAGGGAAAGTTACAT+TGG 0.494880 6_1:-93960730 Msa0899130:CDS
TCTCTGAATTTGTTCACTGA+TGG 0.500195 6_1:+93960918 None:intergenic
TTCTTGTTGGTGCTTCCCCA+AGG 0.507570 6_1:-93963746 Msa0899130:intron
TTATTTCTCCACAAATGTAG+TGG 0.511610 6_1:-93960955 Msa0899130:CDS
GTAAAATTAAATGTGACAAA+AGG 0.511712 6_1:+93960560 None:intergenic
TCGTCTTTAATTTGATGTGT+CGG 0.514689 6_1:+93960473 None:intergenic
CTAAAGAAGAATACTTTGAA+GGG 0.515212 6_1:+93963904 None:intergenic
AGCACATCAACCAGCTGAAG+TGG 0.517198 6_1:+93960666 None:intergenic
ATATAGTGTAGCTCTTTACA+TGG 0.517253 6_1:-93960760 Msa0899130:CDS
CATCAAATTAAAGACGAGTT+CGG 0.519646 6_1:-93960467 Msa0899130:three_prime_UTR
TGGAACAGACCAATCAATAT+GGG 0.528463 6_1:-93964021 Msa0899130:CDS
ACTAACTGCTCCACTTCAGC+TGG 0.538136 6_1:-93960676 Msa0899130:CDS
GAGGTTTGAATAATTGTGCA+TGG 0.540705 6_1:+93960801 None:intergenic
AAATGAATGAAGTGGAAGAG+AGG 0.548449 6_1:+93964192 None:intergenic
TGTTAGACATTAACACGTGC+AGG 0.552655 6_1:+93960442 None:intergenic
GTCACCGCAAAACACTGTCT+TGG 0.557039 6_1:+93960854 None:intergenic
AGTGTTGATCAATTAGTGTC+CGG 0.557674 6_1:-93960391 Msa0899130:three_prime_UTR
ATAAGCCTCTTGAAAAGTTG+AGG 0.571930 6_1:+93963768 None:intergenic
ATGTCTAACATCAACATATG+TGG 0.573967 6_1:-93960429 Msa0899130:three_prime_UTR
GTGGATATGAATCTTAACAT+TGG 0.582169 6_1:-93960229 Msa0899130:three_prime_UTR
GCAAAACACTGTCTTGGCTA+AGG 0.590734 6_1:+93960860 None:intergenic
AGATTCATATCCACCTAATG+TGG 0.591947 6_1:+93960238 None:intergenic
ACAACCAATCATACCAGACA+TGG 0.594195 6_1:+93963809 None:intergenic
ACTGTCTTGGCTAAGGACAA+TGG 0.594937 6_1:+93960867 None:intergenic
CTAAACATAGCTAAATTCAA+GGG 0.608404 6_1:+93960616 None:intergenic
ACTAGAAACAATAGAATGTG+TGG 0.609328 6_1:+93964217 None:intergenic
TGAAGTGGAGCAGTTAGTTG+AGG 0.612568 6_1:+93960681 None:intergenic
ATAAATACAGATGACTATGA+GGG 0.625313 6_1:-93961047 Msa0899130:CDS
TATTCAAACCTCTAGTGACA+TGG 0.628354 6_1:-93960790 Msa0899130:CDS
TTGAGGAAATGACAGTGTTG+TGG 0.648866 6_1:-93960279 Msa0899130:three_prime_UTR
TGGAACAACCATGTCACTAG+AGG 0.653480 6_1:+93960782 None:intergenic
ACGAAGCATTCATACAAACA+TGG 0.660389 6_1:+93960365 None:intergenic
AATACAGATGACTATGAGGG+AGG 0.668351 6_1:-93961044 Msa0899130:CDS
ATTCATACAAACATGGACAC+CGG 0.676948 6_1:+93960372 None:intergenic
ATGAATGCTTCGTAGATGTG+TGG 0.720291 6_1:-93960355 Msa0899130:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATTTAATAAAAAAAAAAT+TGG - chr6_1:93960791-93960810 Msa0899130:CDS 0.0%
!!! CATAAAATAGTTTAAATTTT+AGG - chr6_1:93961332-93961351 Msa0899130:intron 10.0%
!! TAGTAATTAATATTCTTATA+GGG + chr6_1:93961402-93961421 None:intergenic 10.0%
!!! AAAAGTCATAAAATTTTTTT+TGG - chr6_1:93961569-93961588 Msa0899130:intron 10.0%
!! TAAAGAATAAAATAAGTATA+TGG - chr6_1:93962018-93962037 Msa0899130:intron 10.0%
!!! TAATCAAAATCTTTTTTTTA+TGG - chr6_1:93962115-93962134 Msa0899130:intron 10.0%
!!! TTATTCTTTTTTTTTTTTGA+GGG + chr6_1:93963326-93963345 None:intergenic 10.0%
!!! TTTATTCTTTTTTTTTTTTG+AGG + chr6_1:93963327-93963346 None:intergenic 10.0%
!!! TAGTTTTTTTCAGTAAAATA+AGG + chr6_1:93960452-93960471 None:intergenic 15.0%
!! AGTATATAAAAATATACCTT+GGG + chr6_1:93960709-93960728 None:intergenic 15.0%
!!! AATTTAGAATTGAAATGAAA+TGG - chr6_1:93961271-93961290 Msa0899130:intron 15.0%
!! GTAGTAATTAATATTCTTAT+AGG + chr6_1:93961403-93961422 None:intergenic 15.0%
!! AATTTAGATCTTCAAATTAT+AGG - chr6_1:93961608-93961627 Msa0899130:intron 15.0%
!!! TTATATACTTTTTTTTGTGT+CGG + chr6_1:93962369-93962388 None:intergenic 15.0%
!!! CTCTATATTTTATTTTTTGT+CGG + chr6_1:93962514-93962533 None:intergenic 15.0%
!!! TATTTTTATTTGTTAGTTAG+AGG + chr6_1:93962548-93962567 None:intergenic 15.0%
!! ATAATTATAGTGATATAAGT+GGG + chr6_1:93962592-93962611 None:intergenic 15.0%
!! AATAATTATAGTGATATAAG+TGG + chr6_1:93962593-93962612 None:intergenic 15.0%
!!! AACTTTTAATAAAATGTGAT+TGG - chr6_1:93962621-93962640 Msa0899130:intron 15.0%
!! AATAGATAATTATAAACATG+TGG + chr6_1:93962725-93962744 None:intergenic 15.0%
!! GTTAAAATAAAATAATGTGA+TGG - chr6_1:93963199-93963218 Msa0899130:intron 15.0%
!! TCTTTCTCTTTCTTTAATTA+TGG - chr6_1:93960283-93960302 Msa0899130:three_prime_UTR 20.0%
!! GTATATAAAAATATACCTTG+GGG + chr6_1:93960708-93960727 None:intergenic 20.0%
!! GAGTATATAAAAATATACCT+TGG + chr6_1:93960710-93960729 None:intergenic 20.0%
!! ACAATAACTTTACATTTCAA+AGG + chr6_1:93961050-93961069 None:intergenic 20.0%
!!! ATGTAAAGTTATTGTTTTCT+AGG - chr6_1:93961055-93961074 Msa0899130:intron 20.0%
!! AAATGACAAATGTTAAGAAA+GGG + chr6_1:93961192-93961211 None:intergenic 20.0%
!! AAAATGACAAATGTTAAGAA+AGG + chr6_1:93961193-93961212 None:intergenic 20.0%
!!! AACTATTTTATGATACTACA+AGG + chr6_1:93961324-93961343 None:intergenic 20.0%
!!! TAGTTTAAATTTTAGGGAAT+TGG - chr6_1:93961339-93961358 Msa0899130:intron 20.0%
!! AATTAATATTCTTATAGGGA+TGG + chr6_1:93961398-93961417 None:intergenic 20.0%
!! TAGTAAAAAGGAAAATACTT+TGG + chr6_1:93961525-93961544 None:intergenic 20.0%
!! AAACTAGAGATTTAGTAAAA+AGG + chr6_1:93961537-93961556 None:intergenic 20.0%
!!! GTCATAAAATTTTTTTTGGA+AGG - chr6_1:93961573-93961592 Msa0899130:intron 20.0%
!!! CACTGAAATATTTTTTAGAA+AGG - chr6_1:93961707-93961726 Msa0899130:intron 20.0%
!! AGAATAAAATAAGTATATGG+TGG - chr6_1:93962021-93962040 Msa0899130:intron 20.0%
!!! AACAAAACTTTGACTTTAAA+AGG + chr6_1:93962230-93962249 None:intergenic 20.0%
!!! CTATAAAAAACTTCTAGTTT+TGG - chr6_1:93962255-93962274 Msa0899130:intron 20.0%
!! TAATTATAGTGATATAAGTG+GGG + chr6_1:93962591-93962610 None:intergenic 20.0%
!! TATTAAAAGATAAACGTCTT+AGG + chr6_1:93963259-93963278 None:intergenic 20.0%
!! GTAAAATTAAATGTGACAAA+AGG + chr6_1:93963879-93963898 None:intergenic 20.0%
!!! ATTGTTTGTAGTTTTGAATT+TGG - chr6_1:93964120-93964139 Msa0899130:CDS 20.0%
!!! TTGTTTGTAGTTTTGAATTT+GGG - chr6_1:93964121-93964140 Msa0899130:CDS 20.0%
! AAGAAGAAAAATGAATGAAG+TGG + chr6_1:93960255-93960274 None:intergenic 25.0%
! ACAAAGTTGTTCTATTTGAT+TGG - chr6_1:93960471-93960490 Msa0899130:three_prime_UTR 25.0%
! CTAAAGAAGAATACTTTGAA+GGG + chr6_1:93960535-93960554 None:intergenic 25.0%
! TCTAAAGAAGAATACTTTGA+AGG + chr6_1:93960536-93960555 None:intergenic 25.0%
! CTTAAAGTTGTTAACTTTGT+TGG - chr6_1:93960599-93960618 Msa0899130:three_prime_UTR 25.0%
!!! CTTTGTTTGTTATTGTTACT+TGG - chr6_1:93960951-93960970 Msa0899130:CDS 25.0%
! TTACTACTATATAGATGTGA+TGG - chr6_1:93961416-93961435 Msa0899130:intron 25.0%
! GTAATAGACTATATGAGATA+AGG - chr6_1:93961479-93961498 Msa0899130:intron 25.0%
! AAAAGGAAAATACTTTGGTT+GGG + chr6_1:93961520-93961539 None:intergenic 25.0%
! AAAAAGGAAAATACTTTGGT+TGG + chr6_1:93961521-93961540 None:intergenic 25.0%
! AAATCTTAAGAACAGATACT+TGG + chr6_1:93961823-93961842 None:intergenic 25.0%
!! ATCTGTTCTTAAGATTTTAG+TGG - chr6_1:93961826-93961845 Msa0899130:intron 25.0%
!! TATGTTGTGATTCCTTTTTA+AGG - chr6_1:93961958-93961977 Msa0899130:intron 25.0%
!!! TTTTTAAGGTGAATTTGATG+CGG - chr6_1:93961972-93961991 Msa0899130:intron 25.0%
! ATTTATACAAGGAAATGTCT+TGG + chr6_1:93962687-93962706 None:intergenic 25.0%
! AATCTCTTTGGATTTATACA+AGG + chr6_1:93962698-93962717 None:intergenic 25.0%
!! AAAATAGAGATGTCAACTTT+TGG - chr6_1:93962827-93962846 Msa0899130:intron 25.0%
!!! ATTGGTTTTAATAACACCAA+AGG + chr6_1:93962865-93962884 None:intergenic 25.0%
! AAGTATGTATACTCATTTGT+AGG - chr6_1:93962957-93962976 Msa0899130:intron 25.0%
! TCACATCTTCAATAATTCTT+TGG - chr6_1:93963025-93963044 Msa0899130:intron 25.0%
! CACAATATAAGATGATTCAA+TGG + chr6_1:93963079-93963098 None:intergenic 25.0%
! TATAAATACAGATGACTATG+AGG - chr6_1:93963388-93963407 Msa0899130:intron 25.0%
! ATAAATACAGATGACTATGA+GGG - chr6_1:93963389-93963408 Msa0899130:intron 25.0%
! TAGAATCCAACTATGAAAAT+TGG - chr6_1:93963453-93963472 Msa0899130:intron 25.0%
! AGAATCCAACTATGAAAATT+GGG - chr6_1:93963454-93963473 Msa0899130:intron 25.0%
! TACATGGAGAAGAAAATTAA+AGG - chr6_1:93963692-93963711 Msa0899130:intron 25.0%
! ACATGGAGAAGAAAATTAAA+GGG - chr6_1:93963693-93963712 Msa0899130:intron 25.0%
! AATTAAAGGGAAAGTTACAT+TGG - chr6_1:93963706-93963725 Msa0899130:intron 25.0%
! CTAAACATAGCTAAATTCAA+GGG + chr6_1:93963823-93963842 None:intergenic 25.0%
! ACTAAACATAGCTAAATTCA+AGG + chr6_1:93963824-93963843 None:intergenic 25.0%
!! ATTTGTGTAGTATAGTTCTA+TGG - chr6_1:93963910-93963929 Msa0899130:CDS 25.0%
!!! TGTTTGTAGTTTTGAATTTG+GGG - chr6_1:93964122-93964141 Msa0899130:CDS 25.0%
ACTAGAAACAATAGAATGTG+TGG + chr6_1:93960222-93960241 None:intergenic 30.0%
TTATGCTGTTCTAACATGTT+TGG + chr6_1:93960323-93960342 None:intergenic 30.0%
! GAAACTATAGTTTTTGCTCA+TGG - chr6_1:93960389-93960408 Msa0899130:three_prime_UTR 30.0%
! ATAGTTTTTGCTCATGGTTA+TGG - chr6_1:93960395-93960414 Msa0899130:three_prime_UTR 30.0%
! TGATTTTGTCCCATATTGAT+TGG + chr6_1:93960427-93960446 None:intergenic 30.0%
TCTTTAATAGCACCAGAAAA+AGG + chr6_1:93960497-93960516 None:intergenic 30.0%
AGATGACTTGATCAGTTTAT+TGG - chr6_1:93960565-93960584 Msa0899130:three_prime_UTR 30.0%
GATCAGTTTATTGGATCAAA+TGG - chr6_1:93960574-93960593 Msa0899130:three_prime_UTR 30.0%
! GAAAAGTTGAGGTCTTTTAA+TGG + chr6_1:93960660-93960679 None:intergenic 30.0%
CAAACAAAGAACATAGTTAC+AGG + chr6_1:93960940-93960959 None:intergenic 30.0%
GAGAACAACAAAGGAAAAAA+AGG + chr6_1:93961151-93961170 None:intergenic 30.0%
CTCATATAGTCTATTACAAG+TGG + chr6_1:93961476-93961495 None:intergenic 30.0%
AAAGGAAAATACTTTGGTTG+GGG + chr6_1:93961519-93961538 None:intergenic 30.0%
AATGAACCAACCAAAACATA+TGG - chr6_1:93961883-93961902 Msa0899130:intron 30.0%
GCATCAAATTCACCTTAAAA+AGG + chr6_1:93961973-93961992 None:intergenic 30.0%
TAGATAATGCTAAGATCGTT+AGG - chr6_1:93962062-93962081 Msa0899130:intron 30.0%
AATTCCTTAACATATGCCTT+TGG + chr6_1:93962159-93962178 None:intergenic 30.0%
! AAGACCCTTCTTTTATATGT+GGG - chr6_1:93962313-93962332 Msa0899130:intron 30.0%
AACTCCCACATATAAAAGAA+GGG + chr6_1:93962320-93962339 None:intergenic 30.0%
AAACTCCCACATATAAAAGA+AGG + chr6_1:93962321-93962340 None:intergenic 30.0%
! TTGGTGGTTCAAATTTCAAA+AGG + chr6_1:93962656-93962675 None:intergenic 30.0%
! CCTAAAAGGTTCTTTTTGTT+GGG + chr6_1:93962756-93962775 None:intergenic 30.0%
! ACCTAAAAGGTTCTTTTTGT+TGG + chr6_1:93962757-93962776 None:intergenic 30.0%
!! TTGGTGTCATTAAAAACCTT+TGG - chr6_1:93962846-93962865 Msa0899130:intron 30.0%
TGTGTCTATCTTAGAGAAAT+TGG + chr6_1:93962883-93962902 None:intergenic 30.0%
! AGAAACCCAATTTTCATAGT+TGG + chr6_1:93963462-93963481 None:intergenic 30.0%
! TTATTTCTCCACAAATGTAG+TGG - chr6_1:93963481-93963500 Msa0899130:intron 30.0%
ATGTAAAGAGCTACACTATA+TGG + chr6_1:93963677-93963696 None:intergenic 30.0%
ATATAGTGTAGCTCTTTACA+TGG - chr6_1:93963676-93963695 Msa0899130:intron 30.0%
! TTGGAGATAATTGACACTTT+TGG - chr6_1:93963725-93963744 Msa0899130:intron 30.0%
!! TCGTCTTTAATTTGATGTGT+CGG + chr6_1:93963966-93963985 None:intergenic 30.0%
CATCAAATTAAAGACGAGTT+CGG - chr6_1:93963969-93963988 Msa0899130:CDS 30.0%
ATGTCTAACATCAACATATG+TGG - chr6_1:93964007-93964026 Msa0899130:CDS 30.0%
CCTAATGTGGAAAAAAGTTA+TGG + chr6_1:93964188-93964207 None:intergenic 30.0%
!!! CCATAACTTTTTTCCACATT+AGG - chr6_1:93964185-93964204 Msa0899130:five_prime_UTR 30.0%
!!! TAACTTTTTTCCACATTAGG+TGG - chr6_1:93964188-93964207 Msa0899130:five_prime_UTR 30.0%
GTGGATATGAATCTTAACAT+TGG - chr6_1:93964207-93964226 Msa0899130:five_prime_UTR 30.0%
AAATGAATGAAGTGGAAGAG+AGG + chr6_1:93960247-93960266 None:intergenic 35.0%
!! AACATGTTTGGTTTGGTGTT+TGG + chr6_1:93960311-93960330 None:intergenic 35.0%
CTGTTCTAACATGTTTGGTT+TGG + chr6_1:93960318-93960337 None:intergenic 35.0%
ATGGAACAGACCAATCAATA+TGG - chr6_1:93960414-93960433 Msa0899130:three_prime_UTR 35.0%
TGGAACAGACCAATCAATAT+GGG - chr6_1:93960415-93960434 Msa0899130:three_prime_UTR 35.0%
!!! CTATTTGATTGGCCTTTTTC+TGG - chr6_1:93960482-93960501 Msa0899130:three_prime_UTR 35.0%
!! AAAGACCTCAACTTTTCAAG+AGG - chr6_1:93960663-93960682 Msa0899130:CDS 35.0%
ATAAGCCTCTTGAAAAGTTG+AGG + chr6_1:93960671-93960690 None:intergenic 35.0%
TTCAAGAGGCTTATTCTTGT+TGG - chr6_1:93960677-93960696 Msa0899130:CDS 35.0%
CATTTCAAAGGAGAAAGAAG+AGG + chr6_1:93961038-93961057 None:intergenic 35.0%
ATGTTAGAGGAGAACAACAA+AGG + chr6_1:93961160-93961179 None:intergenic 35.0%
AGGGAATATATGCATGTTAG+AGG + chr6_1:93961173-93961192 None:intergenic 35.0%
!! AATGTTAACTTGTGCTCCAA+AGG - chr6_1:93962140-93962159 Msa0899130:intron 35.0%
! CAAGACCCTTCTTTTATATG+TGG - chr6_1:93962312-93962331 Msa0899130:intron 35.0%
ATTGCAATGCTAAGATCGTT+AGG - chr6_1:93962400-93962419 Msa0899130:intron 35.0%
! ATTTGTTAGTTAGAGGCATG+TGG + chr6_1:93962541-93962560 None:intergenic 35.0%
GTGGTTCAAATTTCAAAAGG+AGG + chr6_1:93962653-93962672 None:intergenic 35.0%
AATTTGAACCACCAATTAGC+CGG - chr6_1:93962661-93962680 Msa0899130:intron 35.0%
ACATGTGGAAGAAATCTCTT+TGG + chr6_1:93962710-93962729 None:intergenic 35.0%
! CCCAACAAAAAGAACCTTTT+AGG - chr6_1:93962753-93962772 Msa0899130:intron 35.0%
TGTATACTCATTTGTAGGTC+AGG - chr6_1:93962962-93962981 Msa0899130:intron 35.0%
ATTTGGATCCACTACATTTG+TGG + chr6_1:93963492-93963511 None:intergenic 35.0%
TTCACTGATGGTTCATCATT+TGG + chr6_1:93963509-93963528 None:intergenic 35.0%
! TCTCTGAATTTGTTCACTGA+TGG + chr6_1:93963521-93963540 None:intergenic 35.0%
GAGGTTTGAATAATTGTGCA+TGG + chr6_1:93963638-93963657 None:intergenic 35.0%
TATTCAAACCTCTAGTGACA+TGG - chr6_1:93963646-93963665 Msa0899130:intron 35.0%
!!! ATGTGCTTAAGGGTGTTTTA+GGG - chr6_1:93963786-93963805 Msa0899130:CDS 35.0%
!! AGTGTTGATCAATTAGTGTC+CGG - chr6_1:93964045-93964064 Msa0899130:CDS 35.0%
ATTCATACAAACATGGACAC+CGG + chr6_1:93964067-93964086 None:intergenic 35.0%
! ACGAAGCATTCATACAAACA+TGG + chr6_1:93964074-93964093 None:intergenic 35.0%
! TGGGGTGTTAATTAGATTTG+AGG - chr6_1:93964140-93964159 Msa0899130:exon 35.0%
AGATTCATATCCACCTAATG+TGG + chr6_1:93964201-93964220 None:intergenic 35.0%
CTTCTGAATGCTAGAATCCT+AGG - chr6_1:93960356-93960375 Msa0899130:three_prime_UTR 40.0%
AATGCTAGAATCCTAGGAAC+AGG - chr6_1:93960362-93960381 Msa0899130:three_prime_UTR 40.0%
TAGTTTCTGTTCCTGTTCCT+AGG + chr6_1:93960376-93960395 None:intergenic 40.0%
TTTGTTGGTCATTCCATGTC+TGG - chr6_1:93960614-93960633 Msa0899130:exon 40.0%
CATTCCATGTCTGGTATGAT+TGG - chr6_1:93960623-93960642 Msa0899130:exon 40.0%
ACAACCAATCATACCAGACA+TGG + chr6_1:93960630-93960649 None:intergenic 40.0%
TTCTTCCCAAAATACTGCCT+AGG - chr6_1:93961368-93961387 Msa0899130:intron 40.0%
! GAAGTCCTAGGCAGTATTTT+GGG + chr6_1:93961376-93961395 None:intergenic 40.0%
TACAAGTGGCCTAGTTCATT+TGG + chr6_1:93961462-93961481 None:intergenic 40.0%
!!! CTGACACCATATGTTTTGGT+TGG + chr6_1:93961892-93961911 None:intergenic 40.0%
! CCTACTGACACCATATGTTT+TGG + chr6_1:93961896-93961915 None:intergenic 40.0%
! CCAAAACATATGGTGTCAGT+AGG - chr6_1:93961893-93961912 Msa0899130:intron 40.0%
! ACATATGGTGTCAGTAGGAT+TGG - chr6_1:93961898-93961917 Msa0899130:intron 40.0%
! CATATGGTGTCAGTAGGATT+GGG - chr6_1:93961899-93961918 Msa0899130:intron 40.0%
! TGCTCCAAAGGCATATGTTA+AGG - chr6_1:93962152-93962171 Msa0899130:intron 40.0%
!! ATTCCTTAACTTGTGCCCTT+AGG - chr6_1:93962278-93962297 Msa0899130:intron 40.0%
!! TTCCTTAACTTGTGCCCTTA+GGG - chr6_1:93962279-93962298 Msa0899130:intron 40.0%
! CTTGTTATCTTGTGCCCTAA+GGG + chr6_1:93962296-93962315 None:intergenic 40.0%
! TCTTGTTATCTTGTGCCCTA+AGG + chr6_1:93962297-93962316 None:intergenic 40.0%
ATACAAGGAAATGTCTTGGC+CGG + chr6_1:93962683-93962702 None:intergenic 40.0%
!! AGAACCTTTTAGGTACAGCT+AGG - chr6_1:93962763-93962782 Msa0899130:intron 40.0%
CTTTCCTAGCTGTACCTAAA+AGG + chr6_1:93962770-93962789 None:intergenic 40.0%
CATCTGATGTCAAGTGTGAA+CGG + chr6_1:93962990-93963009 None:intergenic 40.0%
AATACAGATGACTATGAGGG+AGG - chr6_1:93963392-93963411 Msa0899130:intron 40.0%
! TTAGCCAAGACAGTGTTTTG+CGG - chr6_1:93963578-93963597 Msa0899130:intron 40.0%
!!! GATGTGCTTAAGGGTGTTTT+AGG - chr6_1:93963785-93963804 Msa0899130:CDS 40.0%
TGTTAGACATTAACACGTGC+AGG + chr6_1:93963997-93964016 None:intergenic 40.0%
ATGAATGCTTCGTAGATGTG+TGG - chr6_1:93964081-93964100 Msa0899130:CDS 40.0%
TTGAGGAAATGACAGTGTTG+TGG - chr6_1:93964157-93964176 Msa0899130:five_prime_UTR 40.0%
! GGAAGTCCTAGGCAGTATTT+TGG + chr6_1:93961377-93961396 None:intergenic 45.0%
CTTATAGGGATGGAAGTCCT+AGG + chr6_1:93961388-93961407 None:intergenic 45.0%
CACCTCATGCCAAATGAACT+AGG - chr6_1:93961450-93961469 Msa0899130:intron 45.0%
TAAGGTGAATTTGATGCGGC+TGG - chr6_1:93961976-93961995 Msa0899130:intron 45.0%
AAATGTCTTGGCCGGCTAAT+TGG + chr6_1:93962675-93962694 None:intergenic 45.0%
ACTGTCTTGGCTAAGGACAA+TGG + chr6_1:93963572-93963591 None:intergenic 45.0%
GCAAAACACTGTCTTGGCTA+AGG + chr6_1:93963579-93963598 None:intergenic 45.0%
TGGAACAACCATGTCACTAG+AGG + chr6_1:93963657-93963676 None:intergenic 45.0%
TGAAGTGGAGCAGTTAGTTG+AGG + chr6_1:93963758-93963777 None:intergenic 45.0%
! TCAGCTGGTTGATGTGCTTA+AGG - chr6_1:93963775-93963794 Msa0899130:CDS 45.0%
! CAGCTGGTTGATGTGCTTAA+GGG - chr6_1:93963776-93963795 Msa0899130:CDS 45.0%
!!! ATAAAATAGTTTAAATTTTA+GGG - chr6_1:93961333-93961352 Msa0899130:intron 5.0%
!!! TTTAAATTTTATTTAATTTC+AGG + chr6_1:93961653-93961672 None:intergenic 5.0%
!!! ATTTTATTCTTTAATTTTTA+TGG + chr6_1:93962011-93962030 None:intergenic 5.0%
! TTCTTGTTGGTGCTTCCCCA+AGG - chr6_1:93960690-93960709 Msa0899130:CDS 50.0%
GGCCTAGTTCATTTGGCATG+AGG + chr6_1:93961455-93961474 None:intergenic 50.0%
TGTCAGTAGGATTGGGTGAG+AGG - chr6_1:93961906-93961925 Msa0899130:intron 50.0%
GTCAGTAGGATTGGGTGAGA+GGG - chr6_1:93961907-93961926 Msa0899130:intron 50.0%
TCAGTAGGATTGGGTGAGAG+GGG - chr6_1:93961908-93961927 Msa0899130:intron 50.0%
! TGCCCTAAGGGCACAAGTTA+AGG + chr6_1:93962284-93962303 None:intergenic 50.0%
GTCACCGCAAAACACTGTCT+TGG + chr6_1:93963585-93963604 None:intergenic 50.0%
ACTAACTGCTCCACTTCAGC+TGG - chr6_1:93963760-93963779 Msa0899130:CDS 50.0%
AGCACATCAACCAGCTGAAG+TGG + chr6_1:93963773-93963792 None:intergenic 50.0%
TGTCTTGGCCGGCTAATTGG+TGG + chr6_1:93962672-93962691 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr6_1 gene 93960198 93964260 93960198 ID=Msa0899130;Name=Msa0899130
chr6_1 mRNA 93960198 93964260 93960198 ID=Msa0899130-mRNA-1;Parent=Msa0899130;Name=Msa0899130-mRNA-1;_AED=0.21;_eAED=0.21;_QI=104|1|1|1|1|1|2|434|283
chr6_1 exon 93960198 93961073 93960198 ID=Msa0899130-mRNA-1:exon:16007;Parent=Msa0899130-mRNA-1
chr6_1 exon 93963747 93964260 93963747 ID=Msa0899130-mRNA-1:exon:16006;Parent=Msa0899130-mRNA-1
chr6_1 five_prime_UTR 93964157 93964260 93964157 ID=Msa0899130-mRNA-1:five_prime_utr;Parent=Msa0899130-mRNA-1
chr6_1 CDS 93963747 93964156 93963747 ID=Msa0899130-mRNA-1:cds;Parent=Msa0899130-mRNA-1
chr6_1 CDS 93960632 93961073 93960632 ID=Msa0899130-mRNA-1:cds;Parent=Msa0899130-mRNA-1
chr6_1 three_prime_UTR 93960198 93960631 93960198 ID=Msa0899130-mRNA-1:three_prime_utr;Parent=Msa0899130-mRNA-1
Gene Sequence

>Msa0899130

ATGGAACCAAACACCAAACCAAACATGTTAGAACAGCATAACAATCTTTCAACTCTTCTGAATGCTAGAATCCTAGGAACAGGAACAGAAACTATAGTTTTTGCTCATGGTTATGGAACAGACCAATCAATATGGGACAAAATCACACCTTATTTTACTGAAAAAAACTACAAAGTTGTTCTATTTGATTGGCCTTTTTCTGGTGCTATTAAAGATCAAAATCTTTATGACCCTTCAAAGTATTCTTCTTTAGAATCTTTTGCAGATGACTTGATCAGTTTATTGGATCAAATGGAACTTAAAGTTGTTAACTTTGTTGGTCATTCCATGTCTGGTATGATTGGTTGTTTAGCTTCCATTAAAAGACCTCAACTTTTCAAGAGGCTTATTCTTGTTGGTGCTTCCCCAAGATATATAAATACAGATGACTATGAGGGAGGATTCACAAGCTCAGATATTGAGAATTTATTGAAGAACATAGAATCCAACTATGAAAATTGGGTTTCTTATTTCTCCACAAATGTAGTGGATCCAAATGATGAACCATCAGTGAACAAATTCAGAGAGTGTTTGAAAAAAATGAGAAATGAAGTTCCATTGTCCTTAGCCAAGACAGTGTTTTGCGGTGACTATAGAGATATACTTGAGAAAGTTGAAACTCCATGCACAATTATTCAAACCTCTAGTGACATGGTTGTTCCATATAGTGTAGCTCTTTACATGGAGAAGAAAATTAAAGGGAAAGTTACATTGGAGATAATTGACACTTTTGGACATTTTCCTCAACTAACTGCTCCACTTCAGCTGGTTGATGTGCTTAAGGGTGTTTTAGGGTTTGATCACTTCCCTTGA
Protein sequence

>Msa0899130

MEPNTKPNMLEQHNNLSTLLNARILGTGTETIVFAHGYGTDQSIWDKITPYFTEKNYKVVLFDWPFSGAIKDQNLYDPSKYSSLESFADDLISLLDQMELKVVNFVGHSMSGMIGCLASIKRPQLFKRLILVGASPRYINTDDYEGGFTSSDIENLLKNIESNYENWVSYFSTNVVDPNDEPSVNKFRECLKKMRNEVPLSLAKTVFCGDYRDILEKVETPCTIIQTSSDMVVPYSVALYMEKKIKGKVTLEIIDTFGHFPQLTAPLQLVDVLKGVLGFDHFP*