Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1306720 | sp|Q05212|DR102_ARATH | 62.424 | 165 | 57 | 2 | 5 | 169 | 9 | 168 | 3.98e-65 | 205 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1306720 | A0A396GQB3 | 94.681 | 188 | 10 | 0 | 1 | 188 | 50 | 237 | 6.55e-128 | 368 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0043960 | Msa1306720 | 0.817870 | 2.634699e-52 | -8.615850e-47 |
| Msa0044050 | Msa1306720 | 0.811047 | 8.509006e-51 | -8.615850e-47 |
| Msa0090620 | Msa1306720 | 0.812235 | 4.694792e-51 | -8.615850e-47 |
| Msa0619760 | Msa1306720 | 0.801965 | 7.019301e-49 | -8.615850e-47 |
| Msa0716600 | Msa1306720 | 0.816716 | 4.790523e-52 | -8.615850e-47 |
| Msa0798950 | Msa1306720 | -0.800297 | 1.539902e-48 | -8.615850e-47 |
| Msa0176620 | Msa1306720 | 0.812502 | 4.104898e-51 | -8.615850e-47 |
| Msa0183800 | Msa1306720 | 0.817831 | 2.689085e-52 | -8.615850e-47 |
| Msa0223100 | Msa1306720 | -0.811036 | 8.558862e-51 | -8.615850e-47 |
| Msa1258100 | Msa1306720 | -0.801226 | 9.949428e-49 | -8.615850e-47 |
| Msa1306720 | Msa1370930 | 0.832198 | 1.084186e-55 | -8.615850e-47 |
| Msa1306720 | Msa1395690 | 0.809536 | 1.802592e-50 | -8.615850e-47 |
| Msa1306720 | Msa1407210 | 0.804265 | 2.346771e-49 | -8.615850e-47 |
| Msa1306720 | Msa1423550 | -0.812611 | 3.885316e-51 | -8.615850e-47 |
| Msa0405660 | Msa1306720 | 0.815982 | 6.992836e-52 | -8.615850e-47 |
| Msa0427590 | Msa1306720 | -0.807640 | 4.578916e-50 | -8.615850e-47 |
| Msa1006940 | Msa1306720 | 0.805241 | 1.467634e-49 | -8.615850e-47 |
| Msa1030570 | Msa1306720 | 0.804602 | 1.997007e-49 | -8.615850e-47 |
| Msa1036250 | Msa1306720 | -0.815941 | 7.141380e-52 | -8.615850e-47 |
| Msa1039480 | Msa1306720 | 0.808297 | 3.319445e-50 | -8.615850e-47 |
| Msa1076520 | Msa1306720 | -0.805765 | 1.139963e-49 | -8.615850e-47 |
| Msa1085590 | Msa1306720 | 0.866359 | 3.020610e-65 | -8.615850e-47 |
| Msa0277280 | Msa1306720 | 0.805030 | 1.625240e-49 | -8.615850e-47 |
| Msa1214900 | Msa1306720 | -0.813464 | 2.526272e-51 | -8.615850e-47 |
| Msa1219780 | Msa1306720 | 0.810870 | 9.296896e-51 | -8.615850e-47 |
| Msa1222030 | Msa1306720 | 0.807229 | 5.598067e-50 | -8.615850e-47 |
| Msa0546740 | Msa1306720 | -0.805241 | 1.467699e-49 | -8.615850e-47 |
| Msa0575990 | Msa1306720 | 0.869210 | 3.671251e-66 | -8.615850e-47 |
| Msa0576100 | Msa1306720 | 0.868086 | 8.479722e-66 | -8.615850e-47 |
| Msa0964580 | Msa1306720 | -0.804171 | 2.455833e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1306720 | MtrunA17_Chr8g0386801 | 94.681 | 188 | 10 | 0 | 1 | 188 | 50 | 237 | 1.26e-131 | 368 |
| Msa1306720 | MtrunA17_Chr7g0273891 | 77.841 | 176 | 37 | 1 | 1 | 176 | 1 | 174 | 3.59e-89 | 263 |
| Msa1306720 | MtrunA17_Chr7g0273881 | 58.252 | 206 | 65 | 5 | 1 | 188 | 1 | 203 | 6.00e-69 | 208 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1306720 | AT3G04880.1 | 62.424 | 165 | 57 | 2 | 5 | 169 | 9 | 168 | 4.05e-66 | 205 |
Find 53 sgRNAs with CRISPR-Local
Find 58 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTGCAACTGATAATGAATT+AGG | 0.279111 | 8_4:+1638455 | Msa1306720:CDS |
| AAACACGCCAGGAAATTTAT+TGG | 0.309655 | 8_4:-1638177 | None:intergenic |
| CATGGTTTGTTATTGCTTGC+AGG | 0.337090 | 8_4:-1638350 | None:intergenic |
| GCTACCAGACCACGGACTTC+TGG | 0.341674 | 8_4:-1638122 | None:intergenic |
| AGGAAATTTATTGGCGAAGA+TGG | 0.345085 | 8_4:-1638168 | None:intergenic |
| AGAAACTTCTCCATCTCTTC+TGG | 0.360672 | 8_4:-1638374 | None:intergenic |
| ATCTTCAACTTGGATGTTGA+TGG | 0.375151 | 8_4:-1638024 | None:intergenic |
| TTCCGCTGAAGTATAATTGC+CGG | 0.389880 | 8_4:-1638273 | None:intergenic |
| TTCTCCATCTCTTCTGGCCA+TGG | 0.396736 | 8_4:-1638368 | None:intergenic |
| AACATCCAAGTTGAAGATCT+TGG | 0.402414 | 8_4:+1638029 | Msa1306720:CDS |
| GTTGATAGAACGGGCGTTGA+CGG | 0.410559 | 8_4:-1638228 | None:intergenic |
| ATCTTCGCCAATAAATTTCC+TGG | 0.422484 | 8_4:+1638170 | Msa1306720:CDS |
| TATTCCTCGGCCGCTGAAGT+TGG | 0.437328 | 8_4:+1638065 | Msa1306720:CDS |
| AGACAAGTCTCTGGTTGAAA+TGG | 0.438957 | 8_4:+1638397 | Msa1306720:CDS |
| GTTTGAATTGTTGATAGAAC+GGG | 0.444933 | 8_4:-1638237 | None:intergenic |
| GTAGCATGTGGCACCGGTGC+CGG | 0.465413 | 8_4:+1638140 | Msa1306720:CDS |
| CGGGCGTTGACGGCATCAGC+AGG | 0.481374 | 8_4:-1638218 | None:intergenic |
| TCAAACGTCCTCGCCGTCTC+CGG | 0.491868 | 8_4:+1638254 | Msa1306720:CDS |
| CAAGTGACGGCAAACACGCC+AGG | 0.494536 | 8_4:-1638188 | None:intergenic |
| ACGACCAACTTCAGCGGCCG+AGG | 0.501219 | 8_4:-1638069 | None:intergenic |
| CTCACCAGAAGTCCGTGGTC+TGG | 0.504206 | 8_4:+1638118 | Msa1306720:CDS |
| CAAACCATGGCCAGAAGAGA+TGG | 0.509426 | 8_4:+1638364 | Msa1306720:CDS |
| TTGTTATTGCTTGCAGGACA+AGG | 0.512602 | 8_4:-1638344 | None:intergenic |
| GAAGTTTCTAGACAAGTCTC+TGG | 0.519385 | 8_4:+1638388 | Msa1306720:CDS |
| GGAGCGGAGGTAGGAGACTA+AGG | 0.520714 | 8_4:-1638003 | None:intergenic |
| AACTATCACACTCACTCACT+TGG | 0.521077 | 8_4:-1638486 | None:intergenic |
| CAAGTCTCTGGTTGAAATGG+TGG | 0.527841 | 8_4:+1638400 | Msa1306720:CDS |
| GAAATGGTGGAGATCGGAAA+AGG | 0.530827 | 8_4:+1638413 | Msa1306720:CDS |
| CGGACTTCTGGTGAGGAAGA+TGG | 0.540080 | 8_4:-1638110 | None:intergenic |
| GCAACTGATAATGAATTAGG+AGG | 0.540967 | 8_4:+1638458 | Msa1306720:CDS |
| GAAGATGGAGACGCCGGCAC+CGG | 0.542021 | 8_4:-1638153 | None:intergenic |
| AAGTTCCAAGATCTTCAACT+TGG | 0.554485 | 8_4:-1638034 | None:intergenic |
| GATGTTGATGGAGCGGAGGT+AGG | 0.554811 | 8_4:-1638012 | None:intergenic |
| CTGGTTGAAATGGTGGAGAT+CGG | 0.556226 | 8_4:+1638407 | Msa1306720:CDS |
| CGTTTGAATTGTTGATAGAA+CGG | 0.560789 | 8_4:-1638238 | None:intergenic |
| GGTCTGGTAGCATGTGGCAC+CGG | 0.575899 | 8_4:+1638134 | Msa1306720:CDS |
| AGCAGGAGTAAGACAAGTGA+CGG | 0.579606 | 8_4:-1638201 | None:intergenic |
| CAACTTGGATGTTGATGGAG+CGG | 0.587254 | 8_4:-1638019 | None:intergenic |
| GATGCTGTAATGATCGGTGC+CGG | 0.589848 | 8_4:-1637967 | None:intergenic |
| TGAAGTATAATTGCCGGAGA+CGG | 0.598273 | 8_4:-1638267 | None:intergenic |
| TATAATTGCCGGAGACGGCG+AGG | 0.601346 | 8_4:-1638262 | None:intergenic |
| GACTCGACGACCAACTTCAG+CGG | 0.619165 | 8_4:-1638075 | None:intergenic |
| GCAGGACAAGGAGACTTGAA+CGG | 0.633520 | 8_4:-1638332 | None:intergenic |
| CTTGGATGTTGATGGAGCGG+AGG | 0.634307 | 8_4:-1638016 | None:intergenic |
| GTCCGTGGTCTGGTAGCATG+TGG | 0.635767 | 8_4:+1638128 | Msa1306720:CDS |
| ATTGGCGAAGATGGAGACGC+CGG | 0.638711 | 8_4:-1638159 | None:intergenic |
| CTCCGGCAATTATACTTCAG+CGG | 0.652831 | 8_4:+1638271 | Msa1306720:CDS |
| TGCCACATGCTACCAGACCA+CGG | 0.658066 | 8_4:-1638130 | None:intergenic |
| CTGCAAGCAATAACAAACCA+TGG | 0.660390 | 8_4:+1638351 | Msa1306720:CDS |
| GAGAGAGATGCTGTAATGAT+CGG | 0.661193 | 8_4:-1637973 | None:intergenic |
| TCTTCCTCACCAGAAGTCCG+TGG | 0.676014 | 8_4:+1638113 | Msa1306720:CDS |
| CAGACCACGGACTTCTGGTG+AGG | 0.678028 | 8_4:-1638117 | None:intergenic |
| CCACGCGTCTACGATCTCGA+TGG | 0.684947 | 8_4:-1638300 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATGATTTTTATATGTTTGTT+GGG | - | chr8_4:1637939-1637958 | None:intergenic | 15.0% |
| !!! | GATGATTTTTATATGTTTGT+TGG | - | chr8_4:1637940-1637959 | None:intergenic | 20.0% |
| ! | AAACATATAAAAATCATCGC+TGG | + | chr8_4:1637942-1637961 | Msa1306720:CDS | 25.0% |
| !! | AACTTCTTTTTACTATTCCT+CGG | + | chr8_4:1638052-1638071 | Msa1306720:CDS | 25.0% |
| !! | ATTGCAACTGATAATGAATT+AGG | + | chr8_4:1638455-1638474 | Msa1306720:CDS | 25.0% |
| ! | GTTTGAATTGTTGATAGAAC+GGG | - | chr8_4:1638240-1638259 | None:intergenic | 30.0% |
| ! | CGTTTGAATTGTTGATAGAA+CGG | - | chr8_4:1638241-1638260 | None:intergenic | 30.0% |
| !! | ATAAAAATCATCGCTGGTTC+CGG | + | chr8_4:1637948-1637967 | Msa1306720:CDS | 35.0% |
| ATCTTCAACTTGGATGTTGA+TGG | - | chr8_4:1638027-1638046 | None:intergenic | 35.0% | |
| ! | AACATCCAAGTTGAAGATCT+TGG | + | chr8_4:1638029-1638048 | Msa1306720:CDS | 35.0% |
| AAGTTCCAAGATCTTCAACT+TGG | - | chr8_4:1638037-1638056 | None:intergenic | 35.0% | |
| AGGAAATTTATTGGCGAAGA+TGG | - | chr8_4:1638171-1638190 | None:intergenic | 35.0% | |
| ATCTTCGCCAATAAATTTCC+TGG | + | chr8_4:1638170-1638189 | Msa1306720:CDS | 35.0% | |
| AAACACGCCAGGAAATTTAT+TGG | - | chr8_4:1638180-1638199 | None:intergenic | 35.0% | |
| ! | GCAACTGATAATGAATTAGG+AGG | + | chr8_4:1638458-1638477 | Msa1306720:CDS | 35.0% |
| GAGAGAGATGCTGTAATGAT+CGG | - | chr8_4:1637976-1637995 | None:intergenic | 40.0% | |
| TGAAGTATAATTGCCGGAGA+CGG | - | chr8_4:1638270-1638289 | None:intergenic | 40.0% | |
| TTCCGCTGAAGTATAATTGC+CGG | - | chr8_4:1638276-1638295 | None:intergenic | 40.0% | |
| ! | TTGTTATTGCTTGCAGGACA+AGG | - | chr8_4:1638347-1638366 | None:intergenic | 40.0% |
| !! | CATGGTTTGTTATTGCTTGC+AGG | - | chr8_4:1638353-1638372 | None:intergenic | 40.0% |
| CTGCAAGCAATAACAAACCA+TGG | + | chr8_4:1638351-1638370 | Msa1306720:CDS | 40.0% | |
| AGAAACTTCTCCATCTCTTC+TGG | - | chr8_4:1638377-1638396 | None:intergenic | 40.0% | |
| ! | GAAGTTTCTAGACAAGTCTC+TGG | + | chr8_4:1638388-1638407 | Msa1306720:CDS | 40.0% |
| AGACAAGTCTCTGGTTGAAA+TGG | + | chr8_4:1638397-1638416 | Msa1306720:CDS | 40.0% | |
| ! | CAACTTGGATGTTGATGGAG+CGG | - | chr8_4:1638022-1638041 | None:intergenic | 45.0% |
| AGCAGGAGTAAGACAAGTGA+CGG | - | chr8_4:1638204-1638223 | None:intergenic | 45.0% | |
| CTCCGGCAATTATACTTCAG+CGG | + | chr8_4:1638271-1638290 | Msa1306720:CDS | 45.0% | |
| CAAGTCTCTGGTTGAAATGG+TGG | + | chr8_4:1638400-1638419 | Msa1306720:CDS | 45.0% | |
| ! | CTGGTTGAAATGGTGGAGAT+CGG | + | chr8_4:1638407-1638426 | Msa1306720:CDS | 45.0% |
| ! | GAAATGGTGGAGATCGGAAA+AGG | + | chr8_4:1638413-1638432 | Msa1306720:CDS | 45.0% |
| GATGCTGTAATGATCGGTGC+CGG | - | chr8_4:1637970-1637989 | None:intergenic | 50.0% | |
| !! | GTTGATAGAACGGGCGTTGA+CGG | - | chr8_4:1638231-1638250 | None:intergenic | 50.0% |
| GCAGGACAAGGAGACTTGAA+CGG | - | chr8_4:1638335-1638354 | None:intergenic | 50.0% | |
| CAAACCATGGCCAGAAGAGA+TGG | + | chr8_4:1638364-1638383 | Msa1306720:CDS | 50.0% | |
| TTCTCCATCTCTTCTGGCCA+TGG | - | chr8_4:1638371-1638390 | None:intergenic | 50.0% | |
| ! | GATGTTGATGGAGCGGAGGT+AGG | - | chr8_4:1638015-1638034 | None:intergenic | 55.0% |
| ! | CTTGGATGTTGATGGAGCGG+AGG | - | chr8_4:1638019-1638038 | None:intergenic | 55.0% |
| TATTCCTCGGCCGCTGAAGT+TGG | + | chr8_4:1638065-1638084 | Msa1306720:CDS | 55.0% | |
| GACTCGACGACCAACTTCAG+CGG | - | chr8_4:1638078-1638097 | None:intergenic | 55.0% | |
| CGGACTTCTGGTGAGGAAGA+TGG | - | chr8_4:1638113-1638132 | None:intergenic | 55.0% | |
| TCTTCCTCACCAGAAGTCCG+TGG | + | chr8_4:1638113-1638132 | Msa1306720:CDS | 55.0% | |
| TGCCACATGCTACCAGACCA+CGG | - | chr8_4:1638133-1638152 | None:intergenic | 55.0% | |
| ATTGGCGAAGATGGAGACGC+CGG | - | chr8_4:1638162-1638181 | None:intergenic | 55.0% | |
| TATAATTGCCGGAGACGGCG+AGG | - | chr8_4:1638265-1638284 | None:intergenic | 55.0% | |
| GGAGCGGAGGTAGGAGACTA+AGG | - | chr8_4:1638006-1638025 | None:intergenic | 60.0% | |
| CAGACCACGGACTTCTGGTG+AGG | - | chr8_4:1638120-1638139 | None:intergenic | 60.0% | |
| CTCACCAGAAGTCCGTGGTC+TGG | + | chr8_4:1638118-1638137 | Msa1306720:CDS | 60.0% | |
| GCTACCAGACCACGGACTTC+TGG | - | chr8_4:1638125-1638144 | None:intergenic | 60.0% | |
| GTCCGTGGTCTGGTAGCATG+TGG | + | chr8_4:1638128-1638147 | Msa1306720:CDS | 60.0% | |
| GGTCTGGTAGCATGTGGCAC+CGG | + | chr8_4:1638134-1638153 | Msa1306720:CDS | 60.0% | |
| CAAGTGACGGCAAACACGCC+AGG | - | chr8_4:1638191-1638210 | None:intergenic | 60.0% | |
| TCAAACGTCCTCGCCGTCTC+CGG | + | chr8_4:1638254-1638273 | Msa1306720:CDS | 60.0% | |
| CCACGCGTCTACGATCTCGA+TGG | - | chr8_4:1638303-1638322 | None:intergenic | 60.0% | |
| CCATCGAGATCGTAGACGCG+TGG | + | chr8_4:1638300-1638319 | Msa1306720:CDS | 60.0% | |
| ACGACCAACTTCAGCGGCCG+AGG | - | chr8_4:1638072-1638091 | None:intergenic | 65.0% | |
| !! | GTAGCATGTGGCACCGGTGC+CGG | + | chr8_4:1638140-1638159 | Msa1306720:CDS | 65.0% |
| GAAGATGGAGACGCCGGCAC+CGG | - | chr8_4:1638156-1638175 | None:intergenic | 65.0% | |
| !! | CGGGCGTTGACGGCATCAGC+AGG | - | chr8_4:1638221-1638240 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_4 | gene | 1637927 | 1638493 | 1637927 | ID=Msa1306720;Name=Msa1306720 |
| chr8_4 | mRNA | 1637927 | 1638493 | 1637927 | ID=Msa1306720-mRNA-1;Parent=Msa1306720;Name=Msa1306720-mRNA-1;_AED=0.09;_eAED=0.09;_QI=0|-1|0|1|-1|1|1|0|188 |
| chr8_4 | exon | 1637927 | 1638493 | 1637927 | ID=Msa1306720-mRNA-1:exon:444;Parent=Msa1306720-mRNA-1 |
| chr8_4 | CDS | 1637927 | 1638493 | 1637927 | ID=Msa1306720-mRNA-1:cds;Parent=Msa1306720-mRNA-1 |
| Gene Sequence |
| Protein sequence |
