AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1027180

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 77 sgRNAs with CRISPR-Local

Find 250 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAATGCTAATTATAGTTTA+TGG 0.086369 7_1:+59484138 Msa1027180:three_prime_UTR
TTAATTTAATCTTGAAAATT+TGG 0.122950 7_1:-59484425 None:intergenic
TAAATTAAAATTTGATTATT+TGG 0.145260 7_1:-59484460 None:intergenic
AAGAAAGCACCTGTTATTTA+TGG 0.167139 7_1:+59482999 Msa1027180:CDS
AGATTAAATTAATGAAAATT+TGG 0.174794 7_1:+59484436 Msa1027180:three_prime_UTR
GAAATAGCTCCATTTATATA+AGG 0.225284 7_1:+59484054 Msa1027180:three_prime_UTR
AAAATTTCTCGGGCTTTATA+TGG 0.250180 7_1:+59483671 Msa1027180:three_prime_UTR
TAAAATTCGCTATATTTATA+AGG 0.270635 7_1:-59484180 None:intergenic
AGAGCTACTCATCAAATTTC+AGG 0.270993 7_1:-59483032 None:intergenic
TTGATTGCTATAGTTTATAT+AGG 0.278090 7_1:+59484080 Msa1027180:three_prime_UTR
AATCAAAACCCTTATATAAA+TGG 0.298325 7_1:-59484063 None:intergenic
GCAGATCACCTGGTCTTAAA+TGG 0.311454 7_1:+59483876 Msa1027180:three_prime_UTR
GAATGCTAATTATAGTTTAT+GGG 0.313694 7_1:+59484139 Msa1027180:three_prime_UTR
TTACATATCTTGTATAGAAT+TGG 0.314853 7_1:+59483468 Msa1027180:three_prime_UTR
AATAAATAATTAAAATTTCT+CGG 0.342425 7_1:+59483660 Msa1027180:three_prime_UTR
ATTACATAAAGGAGAGAATA+TGG 0.343599 7_1:+59482964 Msa1027180:CDS
CAAGATACTGCCATTGAACT+TGG 0.379589 7_1:+59480159 Msa1027180:CDS
AGAAAGCACCTGTTATTTAT+GGG 0.379944 7_1:+59483000 Msa1027180:CDS
AAATAGCTCCATTTATATAA+GGG 0.383955 7_1:+59484055 Msa1027180:three_prime_UTR
GCTTTCTTCCCATAAATAAC+AGG 0.391981 7_1:-59483008 None:intergenic
TGATTGCTATAGTTTATATA+GGG 0.412634 7_1:+59484081 Msa1027180:three_prime_UTR
TACGTGGTGAAAATATTTAT+AGG 0.413993 7_1:-59484242 None:intergenic
ATAAATAATTAAAATTTCTC+GGG 0.419506 7_1:+59483661 Msa1027180:three_prime_UTR
TAATCTGGCTTAACATAGAA+AGG 0.421828 7_1:+59483750 Msa1027180:three_prime_UTR
CCATCATTATTTCTAAGAAT+AGG 0.428755 7_1:-59483937 None:intergenic
GATAACAAAGGATTACATAA+AGG 0.436486 7_1:+59482953 Msa1027180:CDS
TTCAGCTGGATGGCATGATA+AGG 0.436569 7_1:+59483597 Msa1027180:three_prime_UTR
GGCTTTATATGGAGAAGGTA+AGG 0.443686 7_1:+59483682 Msa1027180:three_prime_UTR
GCAAGTGGTGAACTCTAAAC+AGG 0.462787 7_1:-59483056 None:intergenic
CCTATTCTTAGAAATAATGA+TGG 0.465042 7_1:+59483937 Msa1027180:three_prime_UTR
AAGGAGAGAATATGGCATAA+AGG 0.466216 7_1:+59482972 Msa1027180:CDS
TACAATGATATAGGAGATTG+TGG 0.467972 7_1:+59482926 Msa1027180:intron
AATAATGATGGGTTATTAAG+CGG 0.473078 7_1:+59483949 Msa1027180:three_prime_UTR
TGATCTAAAATTGATGATAC+AGG 0.475870 7_1:-59483536 None:intergenic
TAATTCATTTCCAAGTTCAA+TGG 0.482603 7_1:-59480169 None:intergenic
AATGCTATTGAATATACAAA+TGG 0.489437 7_1:+59483416 Msa1027180:three_prime_UTR
AAACTGCTAGCAAACTATGA+AGG 0.493697 7_1:+59483572 Msa1027180:three_prime_UTR
TCAGCTGGATGGCATGATAA+GGG 0.496675 7_1:+59483598 Msa1027180:three_prime_UTR
GCGGCAGAGGGCAGAAGGTG+TGG 0.498337 7_1:-59483809 None:intergenic
AGAAGGCGGCAGAGGGCAGA+AGG 0.499716 7_1:-59483814 None:intergenic
CAAAGAGGCAAGAAATTACA+TGG 0.507736 7_1:+59483391 Msa1027180:intron
GTTCACCACTTGCATGATCA+AGG 0.514280 7_1:+59483066 Msa1027180:three_prime_UTR
AACATGTCACAAACTAAATG+TGG 0.515257 7_1:+59484389 Msa1027180:three_prime_UTR
AAACTATGAAGGGCTTCAGC+TGG 0.519080 7_1:+59483583 Msa1027180:three_prime_UTR
AAGCAACTGTTTCGCTGATG+TGG 0.519623 7_1:-59484030 None:intergenic
AATGCTAATTATAGTTTATG+GGG 0.519775 7_1:+59484140 Msa1027180:three_prime_UTR
GGTGAGAAGTGCAGATCACC+TGG 0.524126 7_1:+59483866 Msa1027180:three_prime_UTR
GGATTGTCAGAAGGCGGCAG+AGG 0.525146 7_1:-59483822 None:intergenic
GTCCCAATGAGAAAAGTTGT+TGG 0.539840 7_1:+59483493 Msa1027180:three_prime_UTR
TCTCGGGCTTTATATGGAGA+AGG 0.541439 7_1:+59483677 Msa1027180:three_prime_UTR
CTATTCTTAGAAATAATGAT+GGG 0.545280 7_1:+59483938 Msa1027180:three_prime_UTR
GATTGTCAGAAGGCGGCAGA+GGG 0.550966 7_1:-59483821 None:intergenic
TGATTTGGCCATTTAAGACC+AGG 0.558051 7_1:-59483884 None:intergenic
TGATAAGGGTTCAAGTAGGA+TGG 0.559261 7_1:+59483612 Msa1027180:three_prime_UTR
ATAATGATGGGTTATTAAGC+GGG 0.567133 7_1:+59483950 Msa1027180:three_prime_UTR
AACTGCTAGCAAACTATGAA+GGG 0.574265 7_1:+59483573 Msa1027180:three_prime_UTR
TATGAAGGGCTTCAGCTGGA+TGG 0.585494 7_1:+59483587 Msa1027180:three_prime_UTR
TGAGACAAAGTTCAAGAGGG+TGG 0.589004 7_1:+59480101 Msa1027180:CDS
ATAATGAGACAAAGTTCAAG+AGG 0.589640 7_1:+59480097 Msa1027180:CDS
TGTAGAATGAAAATAATGGT+AGG 0.594513 7_1:+59479384 None:intergenic
GAGACAAAGTTCAAGAGGGT+GGG 0.600020 7_1:+59480102 Msa1027180:CDS
CATAGAAAGGCTTTATACGA+GGG 0.610134 7_1:+59483763 Msa1027180:three_prime_UTR
AGCCTGTGTGGATTGTCAGA+AGG 0.620100 7_1:-59483831 None:intergenic
GCTTTATATGGAGAAGGTAA+GGG 0.623958 7_1:+59483683 Msa1027180:three_prime_UTR
ACACAGGCTGATCATCCACT+TGG 0.630161 7_1:+59483845 Msa1027180:three_prime_UTR
CGCCTTCTGACAATCCACAC+AGG 0.642397 7_1:+59483829 Msa1027180:three_prime_UTR
CTGTGTGGATTGTCAGAAGG+CGG 0.647153 7_1:-59483828 None:intergenic
GATTGTGGTAAAGATAACAA+AGG 0.647801 7_1:+59482941 Msa1027180:CDS
ACATAGAAAGGCTTTATACG+AGG 0.656319 7_1:+59483762 Msa1027180:three_prime_UTR
TCTAAAATTGATGATACAGG+TGG 0.658257 7_1:-59483533 None:intergenic
TAATGAGACAAAGTTCAAGA+GGG 0.659889 7_1:+59480098 Msa1027180:CDS
TATCTTGATAGCTACTCGCA+AGG 0.663358 7_1:-59480143 None:intergenic
GGCATGATAAGGGTTCAAGT+AGG 0.663612 7_1:+59483608 Msa1027180:three_prime_UTR
GATTACCTTGATCATGCAAG+TGG 0.664617 7_1:-59483071 None:intergenic
GATCTGCACTTCTCACCAAG+TGG 0.671647 7_1:-59483860 None:intergenic
AAGTGGATGATCAGCCTGTG+TGG 0.676233 7_1:-59483843 None:intergenic
TAACAAGGACTAAAATTACG+TGG 0.721270 7_1:-59484258 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGTTTAAAATTTATTTTGAA+AGG - chr7_1:59483295-59483314 None:intergenic 10.0%
!! ATAAATAATTAAAATTTCTC+GGG + chr7_1:59483661-59483680 Msa1027180:three_prime_UTR 10.0%
!!! AATATTTTTAATTCTTGATT+TGG - chr7_1:59483902-59483921 None:intergenic 10.0%
!!! TTTGTTTAAAGTTTTTAATA+GGG - chr7_1:59483981-59484000 None:intergenic 10.0%
!!! TTTTGTTTAAAGTTTTTAAT+AGG - chr7_1:59483982-59484001 None:intergenic 10.0%
!! TTAATTTAATCTTGAAAATT+TGG - chr7_1:59484428-59484447 None:intergenic 10.0%
!! AGATTAAATTAATGAAAATT+TGG + chr7_1:59484436-59484455 Msa1027180:three_prime_UTR 10.0%
!!! GAATTTTATACTTAACTAAT+TGG + chr7_1:59479517-59479536 Msa1027180:intron 15.0%
!! ATGACTATTAAAATATTGTA+TGG - chr7_1:59479753-59479772 None:intergenic 15.0%
!! ATGTCATATTTATATACAAA+TGG - chr7_1:59479814-59479833 None:intergenic 15.0%
!!! TTTTTGTTTTTTTTTTGATG+TGG + chr7_1:59480275-59480294 Msa1027180:intron 15.0%
!!! TTTTGTTTTTTTTTTGATGT+GGG + chr7_1:59480276-59480295 Msa1027180:intron 15.0%
!!! TTTTATCAATTGAGATTTTT+TGG + chr7_1:59480467-59480486 Msa1027180:intron 15.0%
!!! TTTTGATTTGTTTTGATATA+TGG + chr7_1:59480542-59480561 Msa1027180:intron 15.0%
!! TCTTAATCTATAAAAATTTG+CGG + chr7_1:59480775-59480794 Msa1027180:intron 15.0%
!!! TATTATTTTTAGCTTGAATA+TGG + chr7_1:59481059-59481078 Msa1027180:intron 15.0%
!! ATTATTAAGAGATAAAATAG+AGG + chr7_1:59481257-59481276 Msa1027180:intron 15.0%
!!! ACTTAAAAAAATGAAAGTAT+TGG - chr7_1:59481621-59481640 None:intergenic 15.0%
!! TATAGTAAAAATTTCATTGT+AGG + chr7_1:59481725-59481744 Msa1027180:intron 15.0%
!!! TTATGAATAAAGTATTTAAG+TGG + chr7_1:59481855-59481874 Msa1027180:intron 15.0%
!! TGAAATTTAAATATGATCTA+TGG - chr7_1:59482559-59482578 None:intergenic 15.0%
!!! GATTACATTTTTGTTTTTTA+CGG + chr7_1:59483720-59483739 Msa1027180:three_prime_UTR 15.0%
!!! ATTACATTTTTGTTTTTTAC+GGG + chr7_1:59483721-59483740 Msa1027180:three_prime_UTR 15.0%
!! TAAAATTCGCTATATTTATA+AGG - chr7_1:59484183-59484202 None:intergenic 15.0%
!!! GTGTTATTTTCAATTACTTT+TGG + chr7_1:59479985-59480004 Msa1027180:intron 20.0%
!! ACAAAATTTAGAAATACTCA+TGG - chr7_1:59480034-59480053 None:intergenic 20.0%
!! GAGAAAAAAACTTAATATGA+GGG - chr7_1:59480219-59480238 None:intergenic 20.0%
!! AGAGAAAAAAACTTAATATG+AGG - chr7_1:59480220-59480239 None:intergenic 20.0%
!! ATGCAAAAAAGAAAAGTAAA+AGG - chr7_1:59480405-59480424 None:intergenic 20.0%
!! TATTACAAAGATAAGTAAGA+TGG + chr7_1:59480640-59480659 Msa1027180:intron 20.0%
!! ATCGTTTAAATCATAATTTC+TGG + chr7_1:59480727-59480746 Msa1027180:intron 20.0%
!! CTTAATCTATAAAAATTTGC+GGG + chr7_1:59480776-59480795 Msa1027180:intron 20.0%
!! TGTTTAGATAAACAAAATAC+TGG + chr7_1:59480957-59480976 Msa1027180:intron 20.0%
!!! TATGGTTTTTGATTGTTTTA+TGG + chr7_1:59481077-59481096 Msa1027180:intron 20.0%
!!! TTAAGGTATAACATCTTTTT+TGG - chr7_1:59481408-59481427 None:intergenic 20.0%
!! AGAAAATGAAGATGTTAAAT+AGG + chr7_1:59481560-59481579 Msa1027180:intron 20.0%
!! AATTAATCAAAATCCAAGTT+AGG - chr7_1:59482098-59482117 None:intergenic 20.0%
!!! AGTTTAGAATCATATGTTAA+TGG + chr7_1:59482224-59482243 Msa1027180:intron 20.0%
!!! AAAATTTTCTATGTAACCTT+TGG + chr7_1:59482257-59482276 Msa1027180:intron 20.0%
!! TCACTAAAAATATACCAAAT+TGG - chr7_1:59482509-59482528 None:intergenic 20.0%
!! TTACTACCAATAAACAATTT+GGG - chr7_1:59482750-59482769 None:intergenic 20.0%
!! ATTACTACCAATAAACAATT+TGG - chr7_1:59482751-59482770 None:intergenic 20.0%
!!! CTGAAATTTTACAATGATAT+AGG + chr7_1:59482917-59482936 Msa1027180:intron 20.0%
!!! TTTTAATTTTTATGCAACCA+AGG - chr7_1:59483240-59483259 None:intergenic 20.0%
!! AATGCTATTGAATATACAAA+TGG + chr7_1:59483416-59483435 Msa1027180:three_prime_UTR 20.0%
!! TTACATATCTTGTATAGAAT+TGG + chr7_1:59483468-59483487 Msa1027180:three_prime_UTR 20.0%
!!! TTACATTTTTGTTTTTTACG+GGG + chr7_1:59483722-59483741 Msa1027180:three_prime_UTR 20.0%
!! CTATTCTTAGAAATAATGAT+GGG + chr7_1:59483938-59483957 Msa1027180:three_prime_UTR 20.0%
!! AAATAGCTCCATTTATATAA+GGG + chr7_1:59484055-59484074 Msa1027180:three_prime_UTR 20.0%
!! AATCAAAACCCTTATATAAA+TGG - chr7_1:59484066-59484085 None:intergenic 20.0%
!! TTGATTGCTATAGTTTATAT+AGG + chr7_1:59484080-59484099 Msa1027180:three_prime_UTR 20.0%
!! TGATTGCTATAGTTTATATA+GGG + chr7_1:59484081-59484100 Msa1027180:three_prime_UTR 20.0%
!! TGAATGCTAATTATAGTTTA+TGG + chr7_1:59484138-59484157 Msa1027180:three_prime_UTR 20.0%
!! GAATGCTAATTATAGTTTAT+GGG + chr7_1:59484139-59484158 Msa1027180:three_prime_UTR 20.0%
!! AATGCTAATTATAGTTTATG+GGG + chr7_1:59484140-59484159 Msa1027180:three_prime_UTR 20.0%
!! TCTCATTAAAAAAAAGTGAT+AGG + chr7_1:59484211-59484230 Msa1027180:three_prime_UTR 20.0%
!!! TAAAAAAAAGTGATAGGTTT+TGG + chr7_1:59484217-59484236 Msa1027180:three_prime_UTR 20.0%
! CTTTATGCTTGATTCAAATT+AGG + chr7_1:59479478-59479497 Msa1027180:intron 25.0%
! TCAAATTAGGTTATGTAGTT+AGG + chr7_1:59479491-59479510 Msa1027180:intron 25.0%
! CAAATTAGGTTATGTAGTTA+GGG + chr7_1:59479492-59479511 Msa1027180:intron 25.0%
! AAGTACTTCCATTAAATTGT+CGG - chr7_1:59479944-59479963 None:intergenic 25.0%
! TAATTCATTTCCAAGTTCAA+TGG - chr7_1:59480172-59480191 None:intergenic 25.0%
! ATTCCTTTGTAAATTTCAGT+TGG + chr7_1:59480338-59480357 Msa1027180:intron 25.0%
! TTCCTTTGTAAATTTCAGTT+GGG + chr7_1:59480339-59480358 Msa1027180:intron 25.0%
! AAAAGGTTTGACATGAAAAA+TGG - chr7_1:59480388-59480407 None:intergenic 25.0%
! TCTCTAAATTGACAAGTTTA+AGG - chr7_1:59481425-59481444 None:intergenic 25.0%
! TTAAACTTGTCAATTTAGAG+AGG + chr7_1:59481424-59481443 Msa1027180:intron 25.0%
! TATCATATGCCTTCATAATA+CGG + chr7_1:59481450-59481469 Msa1027180:intron 25.0%
! ATCTCATATCTAATCTTTCA+TGG + chr7_1:59481671-59481690 Msa1027180:intron 25.0%
! ATGAAAGGATTCATAATCTT+TGG + chr7_1:59481949-59481968 Msa1027180:intron 25.0%
!! TATGTAACCTTTGGTTTATT+TGG + chr7_1:59482266-59482285 Msa1027180:intron 25.0%
!!! AGGTAATAAAAGTACCAATT+TGG + chr7_1:59482492-59482511 Msa1027180:intron 25.0%
! GATAACAAAGGATTACATAA+AGG + chr7_1:59482953-59482972 Msa1027180:CDS 25.0%
! ATTACATAAAGGAGAGAATA+TGG + chr7_1:59482964-59482983 Msa1027180:CDS 25.0%
! AAAGGATCATATGATATGTT+GGG - chr7_1:59483106-59483125 None:intergenic 25.0%
! AAAAGGATCATATGATATGT+TGG - chr7_1:59483107-59483126 None:intergenic 25.0%
! AGTAAATTGAATTGGACAAA+AGG - chr7_1:59483124-59483143 None:intergenic 25.0%
! AGTTCTACATAATTGAAGTT+TGG - chr7_1:59483196-59483215 None:intergenic 25.0%
!!! TTCTAATTGTTGTGTTGTTT+TGG + chr7_1:59483326-59483345 Msa1027180:intron 25.0%
!! TGATCTAAAATTGATGATAC+AGG - chr7_1:59483539-59483558 None:intergenic 25.0%
!!! CCATCATTATTTCTAAGAAT+AGG - chr7_1:59483940-59483959 None:intergenic 25.0%
! CCTATTCTTAGAAATAATGA+TGG + chr7_1:59483937-59483956 Msa1027180:three_prime_UTR 25.0%
!!! AATAATGATGGGTTATTAAG+CGG + chr7_1:59483949-59483968 Msa1027180:three_prime_UTR 25.0%
!! TTAAAGTTTTTAATAGGGAG+TGG - chr7_1:59483976-59483995 None:intergenic 25.0%
! GAAATAGCTCCATTTATATA+AGG + chr7_1:59484054-59484073 Msa1027180:three_prime_UTR 25.0%
! TACGTGGTGAAAATATTTAT+AGG - chr7_1:59484245-59484264 None:intergenic 25.0%
TTGTAAGCTTGGAAACATAT+TGG + chr7_1:59479584-59479603 Msa1027180:intron 30.0%
TTGTATGGTACTTACAGTTT+AGG - chr7_1:59479738-59479757 None:intergenic 30.0%
GTCAAAAAACTCTTCATGAA+TGG - chr7_1:59479920-59479939 None:intergenic 30.0%
AGTACTTCCATTAAATTGTC+GGG - chr7_1:59479943-59479962 None:intergenic 30.0%
!! TTAATGGAAGTACTTTCATG+AGG + chr7_1:59479949-59479968 Msa1027180:intron 30.0%
! TGTTTTGTGAAACTTTTAGC+AGG + chr7_1:59480051-59480070 Msa1027180:intron 30.0%
ATAATGAGACAAAGTTCAAG+AGG + chr7_1:59480097-59480116 Msa1027180:CDS 30.0%
TAATGAGACAAAGTTCAAGA+GGG + chr7_1:59480098-59480117 Msa1027180:CDS 30.0%
!!! TTTTTTTGATGTGGGAGAAA+TGG + chr7_1:59480284-59480303 Msa1027180:intron 30.0%
! AAATGGTTACTTTGAACACA+AGG - chr7_1:59480371-59480390 None:intergenic 30.0%
TTACAGTGATACTGTGTATT+TGG - chr7_1:59480899-59480918 None:intergenic 30.0%
TAGTGCAAAACAGAGTTATA+TGG - chr7_1:59481177-59481196 None:intergenic 30.0%
AACTCTCAATGTAGAAAAGA+TGG + chr7_1:59481281-59481300 Msa1027180:intron 30.0%
!! TCTTAGGTGATTTGGTTATT+CGG + chr7_1:59481314-59481333 Msa1027180:intron 30.0%
ATCATATGCCTTCATAATAC+GGG + chr7_1:59481451-59481470 Msa1027180:intron 30.0%
TTCCATCTGCATAAATGAAT+AGG + chr7_1:59481475-59481494 Msa1027180:intron 30.0%
ATCCTATTCATTTATGCAGA+TGG - chr7_1:59481480-59481499 None:intergenic 30.0%
! GTGGAAGTTTTCTTCTTTAT+TGG + chr7_1:59481874-59481893 Msa1027180:intron 30.0%
AATGATCAAGAAGAGATGAA+AGG + chr7_1:59481934-59481953 Msa1027180:intron 30.0%
! AGGATTCATAATCTTTGGAA+TGG + chr7_1:59481954-59481973 Msa1027180:intron 30.0%
! GCTTTTATACTGCACTATAA+AGG - chr7_1:59482373-59482392 None:intergenic 30.0%
AGTGCAGTATAAAAGCTTAT+TGG + chr7_1:59482377-59482396 Msa1027180:intron 30.0%
TTTCAGTTTGAATCTTGTGT+AGG + chr7_1:59482649-59482668 Msa1027180:intron 30.0%
TTCAGTTTGAATCTTGTGTA+GGG + chr7_1:59482650-59482669 Msa1027180:intron 30.0%
CTAATGATTAAGTAATCAGC+AGG - chr7_1:59482710-59482729 None:intergenic 30.0%
AAGTTGCCCAAATTGTTTAT+TGG + chr7_1:59482741-59482760 Msa1027180:intron 30.0%
GAAACACAATGCAAAACATT+TGG + chr7_1:59482800-59482819 Msa1027180:intron 30.0%
TACAATGATATAGGAGATTG+TGG + chr7_1:59482926-59482945 Msa1027180:intron 30.0%
GATTGTGGTAAAGATAACAA+AGG + chr7_1:59482941-59482960 Msa1027180:CDS 30.0%
!! AAGAAAGCACCTGTTATTTA+TGG + chr7_1:59482999-59483018 Msa1027180:CDS 30.0%
!! AGAAAGCACCTGTTATTTAT+GGG + chr7_1:59483000-59483019 Msa1027180:CDS 30.0%
TCCAATTCAATTTACTGCTA+TGG + chr7_1:59483128-59483147 Msa1027180:intron 30.0%
ACCATAGCAGTAAATTGAAT+TGG - chr7_1:59483132-59483151 None:intergenic 30.0%
GTTAAACTACATTTACACCT+TGG + chr7_1:59483220-59483239 Msa1027180:intron 30.0%
! AACCAACAACTTTTCTCATT+GGG - chr7_1:59483498-59483517 None:intergenic 30.0%
! AAACCAACAACTTTTCTCAT+TGG - chr7_1:59483499-59483518 None:intergenic 30.0%
! TCTAAAATTGATGATACAGG+TGG - chr7_1:59483536-59483555 None:intergenic 30.0%
! AAAATTTCTCGGGCTTTATA+TGG + chr7_1:59483671-59483690 Msa1027180:three_prime_UTR 30.0%
!!! ATTTTTGTTTTTTACGGGGA+AGG + chr7_1:59483726-59483745 Msa1027180:three_prime_UTR 30.0%
! TAATCTGGCTTAACATAGAA+AGG + chr7_1:59483750-59483769 Msa1027180:three_prime_UTR 30.0%
!! ATAATGATGGGTTATTAAGC+GGG + chr7_1:59483950-59483969 Msa1027180:three_prime_UTR 30.0%
TAACAAGGACTAAAATTACG+TGG - chr7_1:59484261-59484280 None:intergenic 30.0%
AACATGTCACAAACTAAATG+TGG + chr7_1:59484389-59484408 Msa1027180:three_prime_UTR 30.0%
!! TGAACCTCTAGGATATATCT+GGG + chr7_1:59479544-59479563 Msa1027180:intron 35.0%
GAATGACACAATGGACAAAA+TGG - chr7_1:59479658-59479677 None:intergenic 35.0%
ACACATCATGAATGACACAA+TGG - chr7_1:59479667-59479686 None:intergenic 35.0%
TCTACCAAAACAATCTGATC+TGG - chr7_1:59479889-59479908 None:intergenic 35.0%
! TTTTGACCCCGACAATTTAA+TGG + chr7_1:59479933-59479952 Msa1027180:intron 35.0%
GTACTTCCATTAAATTGTCG+GGG - chr7_1:59479942-59479961 None:intergenic 35.0%
CACCCAACTGAAATTTACAA+AGG - chr7_1:59480344-59480363 None:intergenic 35.0%
! TAATTTCTGGAGACATCTTG+TGG + chr7_1:59480740-59480759 Msa1027180:intron 35.0%
! TGAGAAACTGTCAAAGTGTT+TGG + chr7_1:59480859-59480878 Msa1027180:intron 35.0%
! ACTGTCAAAGTGTTTGGTTA+TGG + chr7_1:59480865-59480884 Msa1027180:intron 35.0%
! CTGTCAAAGTGTTTGGTTAT+GGG + chr7_1:59480866-59480885 Msa1027180:intron 35.0%
AATGAGAGAGAACTCAAAAG+AGG - chr7_1:59480987-59481006 None:intergenic 35.0%
!! AGATGGTAGAGTATTGTCTT+AGG + chr7_1:59481298-59481317 Msa1027180:intron 35.0%
! GAGTATTGTCTTAGGTGATT+TGG + chr7_1:59481306-59481325 Msa1027180:intron 35.0%
!! CTTAGGTGATTTGGTTATTC+GGG + chr7_1:59481315-59481334 Msa1027180:intron 35.0%
!! TTAGGTGATTTGGTTATTCG+GGG + chr7_1:59481316-59481335 Msa1027180:intron 35.0%
AGATACTGTTAACTCTCTCT+TGG - chr7_1:59481383-59481402 None:intergenic 35.0%
AGAAAGAGCAAGTCATTTCA+TGG + chr7_1:59481644-59481663 Msa1027180:intron 35.0%
TTGACCACAAATGTCATAGT+TGG + chr7_1:59481987-59482006 Msa1027180:intron 35.0%
TGACCACAAATGTCATAGTT+GGG + chr7_1:59481988-59482007 Msa1027180:intron 35.0%
ATACCCAACTATGACATTTG+TGG - chr7_1:59481994-59482013 None:intergenic 35.0%
ATCAAAATCCAAGTTAGGCA+AGG - chr7_1:59482093-59482112 None:intergenic 35.0%
GCATAGAGATAAAAGACATC+AGG - chr7_1:59482295-59482314 None:intergenic 35.0%
CTATAAAGGACTTTGTAGGA+AGG - chr7_1:59482359-59482378 None:intergenic 35.0%
TGCACTATAAAGGACTTTGT+AGG - chr7_1:59482363-59482382 None:intergenic 35.0%
TAAACTGCATAACTGACATG+AGG + chr7_1:59482472-59482491 Msa1027180:intron 35.0%
! AAATGTGAGTATTGTGCGTT+GGG - chr7_1:59482850-59482869 None:intergenic 35.0%
!! TAAGAAGGCACTTCTAAAGA+GGG - chr7_1:59482886-59482905 None:intergenic 35.0%
TCAGCATTGTATCAGTAAGA+AGG - chr7_1:59482901-59482920 None:intergenic 35.0%
AAGGAGAGAATATGGCATAA+AGG + chr7_1:59482972-59482991 Msa1027180:CDS 35.0%
GCTTTCTTCCCATAAATAAC+AGG - chr7_1:59483011-59483030 None:intergenic 35.0%
AGAGCTACTCATCAAATTTC+AGG - chr7_1:59483035-59483054 None:intergenic 35.0%
CAAAGAGGCAAGAAATTACA+TGG + chr7_1:59483391-59483410 Msa1027180:intron 35.0%
AAACTGCTAGCAAACTATGA+AGG + chr7_1:59483572-59483591 Msa1027180:three_prime_UTR 35.0%
AACTGCTAGCAAACTATGAA+GGG + chr7_1:59483573-59483592 Msa1027180:three_prime_UTR 35.0%
GCTTTATATGGAGAAGGTAA+GGG + chr7_1:59483683-59483702 Msa1027180:three_prime_UTR 35.0%
! ACATAGAAAGGCTTTATACG+AGG + chr7_1:59483762-59483781 Msa1027180:three_prime_UTR 35.0%
! CATAGAAAGGCTTTATACGA+GGG + chr7_1:59483763-59483782 Msa1027180:three_prime_UTR 35.0%
!! TAATTGGCTTGTGAACCTCT+AGG + chr7_1:59479533-59479552 Msa1027180:intron 40.0%
!! GTGAACCTCTAGGATATATC+TGG + chr7_1:59479543-59479562 Msa1027180:intron 40.0%
TCATTCATGATGTGTGTGTG+TGG + chr7_1:59479672-59479691 Msa1027180:intron 40.0%
AAACAATCTGATCTGGCATG+TGG - chr7_1:59479882-59479901 None:intergenic 40.0%
! CATGCCAGATCAGATTGTTT+TGG + chr7_1:59479882-59479901 Msa1027180:intron 40.0%
! TATCTTGATAGCTACTCGCA+AGG - chr7_1:59480146-59480165 None:intergenic 40.0%
CAAGATACTGCCATTGAACT+TGG + chr7_1:59480159-59480178 Msa1027180:CDS 40.0%
! ACTGTACTTTGTTTCGCTCT+TGG + chr7_1:59480603-59480622 Msa1027180:intron 40.0%
! AAAGTGTTTAGATAGCAGCC+AGG - chr7_1:59480840-59480859 None:intergenic 40.0%
!! TAGGTGATTTGGTTATTCGG+GGG + chr7_1:59481317-59481336 Msa1027180:intron 40.0%
TAATCTTTCATGGTACCTGC+AGG + chr7_1:59481681-59481700 Msa1027180:intron 40.0%
TGTTCAAACCTTGCCTAACT+TGG + chr7_1:59482082-59482101 Msa1027180:intron 40.0%
!!! GCTCTAAGATTTTGCAAGCT+TGG + chr7_1:59482179-59482198 Msa1027180:intron 40.0%
CAGGCATCCAAATAAACCAA+AGG - chr7_1:59482276-59482295 None:intergenic 40.0%
TAGTTGAGGGAGAACTTGAA+AGG + chr7_1:59482418-59482437 Msa1027180:intron 40.0%
GGGAGAACTTGAAAGGAAAA+TGG + chr7_1:59482425-59482444 Msa1027180:intron 40.0%
TATGCAGTTTAGCACTCTCA+TGG - chr7_1:59482463-59482482 None:intergenic 40.0%
TTGAATCTTGTGTAGGGACT+TGG + chr7_1:59482656-59482675 Msa1027180:intron 40.0%
TGAATCTTGTGTAGGGACTT+GGG + chr7_1:59482657-59482676 Msa1027180:intron 40.0%
AGTAATCAGCAGGATTGAGT+GGG - chr7_1:59482700-59482719 None:intergenic 40.0%
AAGTAATCAGCAGGATTGAG+TGG - chr7_1:59482701-59482720 None:intergenic 40.0%
! AATGTGAGTATTGTGCGTTG+GGG - chr7_1:59482849-59482868 None:intergenic 40.0%
! GAAATGTGAGTATTGTGCGT+TGG - chr7_1:59482851-59482870 None:intergenic 40.0%
!! GTAAGAAGGCACTTCTAAAG+AGG - chr7_1:59482887-59482906 None:intergenic 40.0%
GATTACCTTGATCATGCAAG+TGG - chr7_1:59483074-59483093 None:intergenic 40.0%
! GAAGTTTGGAAGCATGTTTG+TGG - chr7_1:59483182-59483201 None:intergenic 40.0%
!! GTAACTTCAACTTGCTGCTA+TGG + chr7_1:59483350-59483369 Msa1027180:intron 40.0%
GTCCCAATGAGAAAAGTTGT+TGG + chr7_1:59483493-59483512 Msa1027180:three_prime_UTR 40.0%
TGATAAGGGTTCAAGTAGGA+TGG + chr7_1:59483612-59483631 Msa1027180:three_prime_UTR 40.0%
! GGCTTTATATGGAGAAGGTA+AGG + chr7_1:59483682-59483701 Msa1027180:three_prime_UTR 40.0%
! TTTTACGGGGAAGGTTAATC+TGG + chr7_1:59483735-59483754 Msa1027180:three_prime_UTR 40.0%
CTCACCCAGATATATCCTAG+AGG - chr7_1:59479551-59479570 None:intergenic 45.0%
CTAGGATATATCTGGGTGAG+TGG + chr7_1:59479551-59479570 Msa1027180:intron 45.0%
GTTTCCAAGCTTACAAACCC+TGG - chr7_1:59479580-59479599 None:intergenic 45.0%
ATCTTCCTAGCAAAGCGAGA+TGG - chr7_1:59479633-59479652 None:intergenic 45.0%
TGGCATGTGGTAATCAGATC+TGG - chr7_1:59479869-59479888 None:intergenic 45.0%
TGAGACAAAGTTCAAGAGGG+TGG + chr7_1:59480101-59480120 Msa1027180:CDS 45.0%
GAGACAAAGTTCAAGAGGGT+GGG + chr7_1:59480102-59480121 Msa1027180:CDS 45.0%
GATGGAAGCCCGTATTATGA+AGG - chr7_1:59481462-59481481 None:intergenic 45.0%
CAATTGCTTGATTGTCCTGC+AGG - chr7_1:59481699-59481718 None:intergenic 45.0%
CATGCACTGCTTGTAGTTGA+GGG + chr7_1:59482405-59482424 Msa1027180:intron 45.0%
GCAAGTGGTGAACTCTAAAC+AGG - chr7_1:59483059-59483078 None:intergenic 45.0%
GTTCACCACTTGCATGATCA+AGG + chr7_1:59483066-59483085 Msa1027180:three_prime_UTR 45.0%
CTTGCTGCTATGGAATCCTA+CGG + chr7_1:59483360-59483379 Msa1027180:intron 45.0%
TGCTATGGAATCCTACGGAT+TGG + chr7_1:59483365-59483384 Msa1027180:intron 45.0%
AAACTATGAAGGGCTTCAGC+TGG + chr7_1:59483583-59483602 Msa1027180:three_prime_UTR 45.0%
TTCAGCTGGATGGCATGATA+AGG + chr7_1:59483597-59483616 Msa1027180:three_prime_UTR 45.0%
TCAGCTGGATGGCATGATAA+GGG + chr7_1:59483598-59483617 Msa1027180:three_prime_UTR 45.0%
GGCATGATAAGGGTTCAAGT+AGG + chr7_1:59483608-59483627 Msa1027180:three_prime_UTR 45.0%
! TCTCGGGCTTTATATGGAGA+AGG + chr7_1:59483677-59483696 Msa1027180:three_prime_UTR 45.0%
! TTCGCTGATGTGGCATTTTG+TGG - chr7_1:59484023-59484042 None:intergenic 45.0%
AAGCAACTGTTTCGCTGATG+TGG - chr7_1:59484033-59484052 None:intergenic 45.0%
!! AATAAATAATTAAAATTTCT+CGG + chr7_1:59483660-59483679 Msa1027180:three_prime_UTR 5.0%
!! AAGAATTAAAAATATTAATT+AGG + chr7_1:59483906-59483925 Msa1027180:three_prime_UTR 5.0%
!!! AAATAAATTTTAATTTAACA+AGG - chr7_1:59484276-59484295 None:intergenic 5.0%
!! TAAATTAAAATTTGATTATT+TGG - chr7_1:59484463-59484482 None:intergenic 5.0%
GCTTCCAGGGTTTGTAAGCT+TGG + chr7_1:59479573-59479592 Msa1027180:intron 50.0%
TCATGCCATCTCGCTTTGCT+AGG + chr7_1:59479625-59479644 Msa1027180:intron 50.0%
TCATGATGTGTGTGTGTGGC+AGG + chr7_1:59479676-59479695 Msa1027180:intron 50.0%
!! CAAGAGGGTGGGTGTTTTTG+TGG + chr7_1:59480113-59480132 Msa1027180:CDS 50.0%
TGCTTGCTTACATGCTTGCC+TGG + chr7_1:59480819-59480838 Msa1027180:intron 50.0%
!! GTGATTTGGTTATTCGGGGG+AGG + chr7_1:59481320-59481339 Msa1027180:intron 50.0%
ATTCGGGGGAGGAAACTTGT+AGG + chr7_1:59481331-59481350 Msa1027180:intron 50.0%
CCTCAACTACAAGCAGTGCA+TGG - chr7_1:59482407-59482426 None:intergenic 50.0%
CCATGCACTGCTTGTAGTTG+AGG + chr7_1:59482404-59482423 Msa1027180:intron 50.0%
CCTCTTTGTCTCCAATCCGT+AGG - chr7_1:59483379-59483398 None:intergenic 50.0%
!! CCTACGGATTGGAGACAAAG+AGG + chr7_1:59483376-59483395 Msa1027180:intron 50.0%
TATGAAGGGCTTCAGCTGGA+TGG + chr7_1:59483587-59483606 Msa1027180:three_prime_UTR 50.0%
! CTGTGTGGATTGTCAGAAGG+CGG - chr7_1:59483831-59483850 None:intergenic 50.0%
! AGCCTGTGTGGATTGTCAGA+AGG - chr7_1:59483834-59483853 None:intergenic 50.0%
AAGTGGATGATCAGCCTGTG+TGG - chr7_1:59483846-59483865 None:intergenic 50.0%
ACACAGGCTGATCATCCACT+TGG + chr7_1:59483845-59483864 Msa1027180:three_prime_UTR 50.0%
GATCTGCACTTCTCACCAAG+TGG - chr7_1:59483863-59483882 None:intergenic 50.0%
TATCTGGGTGAGTGGCTTCC+AGG + chr7_1:59479559-59479578 Msa1027180:intron 55.0%
ATCTGGGTGAGTGGCTTCCA+GGG + chr7_1:59479560-59479579 Msa1027180:intron 55.0%
TGATGTGTGTGTGTGGCAGG+AGG + chr7_1:59479679-59479698 Msa1027180:intron 55.0%
GTGTGTGTGGCAGGAGGATA+TGG + chr7_1:59479685-59479704 Msa1027180:intron 55.0%
GATTGTCAGAAGGCGGCAGA+GGG - chr7_1:59483824-59483843 None:intergenic 55.0%
CGCCTTCTGACAATCCACAC+AGG + chr7_1:59483829-59483848 Msa1027180:three_prime_UTR 55.0%
! GGTGAGAAGTGCAGATCACC+TGG + chr7_1:59483866-59483885 Msa1027180:three_prime_UTR 55.0%
GGATTGTCAGAAGGCGGCAG+AGG - chr7_1:59483825-59483844 None:intergenic 60.0%
AGAAGGCGGCAGAGGGCAGA+AGG - chr7_1:59483817-59483836 None:intergenic 65.0%
GCGGCAGAGGGCAGAAGGTG+TGG - chr7_1:59483812-59483831 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr7_1 gene 59479399 59484483 59479399 ID=Msa1027180;Name=Msa1027180
chr7_1 mRNA 59479399 59484483 59479399 ID=Msa1027180-mRNA-1;Parent=Msa1027180;Name=Msa1027180-mRNA-1;_AED=0.35;_eAED=0.35;_QI=0|0.33|0.75|0.75|0|0|4|1132|76
chr7_1 exon 59479399 59479405 59479399 ID=Msa1027180-mRNA-1:exon:12062;Parent=Msa1027180-mRNA-1
chr7_1 exon 59480073 59480191 59480073 ID=Msa1027180-mRNA-1:exon:12063;Parent=Msa1027180-mRNA-1
chr7_1 exon 59482939 59483087 59482939 ID=Msa1027180-mRNA-1:exon:12064;Parent=Msa1027180-mRNA-1
chr7_1 exon 59483396 59484483 59483396 ID=Msa1027180-mRNA-1:exon:12065;Parent=Msa1027180-mRNA-1
chr7_1 CDS 59479399 59479405 59479399 ID=Msa1027180-mRNA-1:cds;Parent=Msa1027180-mRNA-1
chr7_1 CDS 59480073 59480191 59480073 ID=Msa1027180-mRNA-1:cds;Parent=Msa1027180-mRNA-1
chr7_1 CDS 59482939 59483043 59482939 ID=Msa1027180-mRNA-1:cds;Parent=Msa1027180-mRNA-1
chr7_1 three_prime_UTR 59483044 59483087 59483044 ID=Msa1027180-mRNA-1:three_prime_utr;Parent=Msa1027180-mRNA-1
chr7_1 three_prime_UTR 59483396 59484483 59483396 ID=Msa1027180-mRNA-1:three_prime_utr;Parent=Msa1027180-mRNA-1
Gene Sequence

>Msa1027180

ATGGTAGGTTATCAAATACGAATGAAGACAAATAATGAGACAAAGTTCAAGAGGGTGGGTGTTTTTGTGGAAGTAGTCCTTGCGAGTAGCTATCAAGATACTGCCATTGAACTTGGAAATGAATTAGAGATTGTGGTAAAGATAACAAAGGATTACATAAAGGAGAGAATATGGCATAAAGGAGAGAAGAAAGCACCTGTTATTTATGGGAAGAAAGCACCTGAAATTTGA
Protein sequence

>Msa1027180

MVGYQIRMKTNNETKFKRVGVFVEVVLASSYQDTAIELGNELEIVVKITKDYIKERIWHKGEKKAPVIYGKKAPEI*