AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1053160


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1053160 MtrunA17_Chr7g0223171 95.918 49 2 0 1 49 1 49 6.60e-30 99.0
Msa1053160 MtrunA17_Chr8g0376211 85.714 49 7 0 1 49 1 49 1.55e-27 92.8
Msa1053160 MtrunA17_Chr8g0376251 85.714 49 7 0 1 49 1 49 1.55e-27 92.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1053160 AT1G06515.1 66.038 53 14 1 1 49 1 53 1.30e-18 70.9
Msa1053160 AT1G06515.2 66.038 53 14 1 1 49 1 53 1.30e-18 70.9
Msa1053160 AT2G30942.1 62.264 53 16 1 1 49 1 53 3.48e-17 67.4

Find 15 sgRNAs with CRISPR-Local

Find 143 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTAACTATTACAGATATTT+TGG 0.137656 7_2:+22530316 Msa1053160:intron
CACCTTTACAATGTTACTTT+TGG 0.153503 7_2:+22529987 Msa1053160:CDS
ACAGATCATTCAACATTTAT+TGG 0.178417 7_2:+22527392 Msa1053160:five_prime_UTR
TGAACTACCTCTTGAAGAAT+TGG 0.287014 7_2:-22530376 None:intergenic
GGCTTTATATGCTGGATTGG+TGG 0.387109 7_2:+22530009 Msa1053160:CDS
CAGTTGTGGAAGTCATGAAT+TGG 0.399034 7_2:+22529949 Msa1053160:intron
TTGGGCTTTATATGCTGGAT+TGG 0.428670 7_2:+22530006 Msa1053160:CDS
GCTTTATATGCTGGATTGGT+GGG 0.445941 7_2:+22530010 Msa1053160:CDS
CATGAATTGGGTTCAGCAAA+AGG 0.457099 7_2:+22529962 Msa1053160:exon
AAATCTCATGCGATCGCACC+AGG 0.500256 7_2:+22527422 Msa1053160:five_prime_UTR
GAACTACCTCTTGAAGAATT+GGG 0.501709 7_2:-22530375 None:intergenic
GCCCAAAAGTAACATTGTAA+AGG 0.523226 7_2:-22529989 None:intergenic
AGTTGTGGAAGTCATGAATT+GGG 0.531926 7_2:+22529950 Msa1053160:intron
CAAAAGTAACATTGTAAAGG+TGG 0.589878 7_2:-22529986 None:intergenic
ACATTACCCAATTCTTCAAG+AGG 0.594314 7_2:+22530369 Msa1053160:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTAATTTCTTTTATGTTATT+TGG + chr7_2:22527746-22527765 Msa1053160:intron 10.0%
!! GAAAGAAAAATTAATTTAAG+AGG - chr7_2:22527480-22527499 None:intergenic 15.0%
!!! ATAGTTTTTGTAATTTAAGT+TGG + chr7_2:22528514-22528533 Msa1053160:intron 15.0%
!!! TAGTTTTTGTAATTTAAGTT+GGG + chr7_2:22528515-22528534 Msa1053160:intron 15.0%
!! TAAAATACAAATACTTAATG+AGG - chr7_2:22528811-22528830 None:intergenic 15.0%
!!! TTGTGTAATCTTTAATTTTT+AGG + chr7_2:22529699-22529718 Msa1053160:intron 15.0%
!!! ACTGCAAAAAATATATTTTT+GGG - chr7_2:22529933-22529952 None:intergenic 15.0%
!!! AACTGCAAAAAATATATTTT+TGG - chr7_2:22529934-22529953 None:intergenic 15.0%
!!! ATTATTTGTTGTTTTGTTGA+TGG - chr7_2:22527243-22527262 None:intergenic 20.0%
!!! TATAAAGTTTTGAGTAATGT+TGG + chr7_2:22527620-22527639 Msa1053160:intron 20.0%
!! GAAAACTTTGATTCTTAAAA+AGG - chr7_2:22527838-22527857 None:intergenic 20.0%
!! ACAAATTACAGAATGAAAAA+TGG + chr7_2:22528015-22528034 Msa1053160:intron 20.0%
!!! TTTAAAACTATTGTTTTTGC+AGG - chr7_2:22528159-22528178 None:intergenic 20.0%
!! GAAAATATCACATCTAATAA+GGG - chr7_2:22528404-22528423 None:intergenic 20.0%
!! AGAAAATATCACATCTAATA+AGG - chr7_2:22528405-22528424 None:intergenic 20.0%
!!! ATTAGATGTGATATTTTCTT+AGG + chr7_2:22528406-22528425 Msa1053160:intron 20.0%
!! GAGTAATGAACATAATTAAA+AGG - chr7_2:22528670-22528689 None:intergenic 20.0%
!!! TTACTCTTTGATTTTGTAAT+TGG + chr7_2:22528684-22528703 Msa1053160:intron 20.0%
!!! TTTTGCATTTCTATATATAC+TGG + chr7_2:22529869-22529888 Msa1053160:intron 20.0%
!!! CTGCAAAAAATATATTTTTG+GGG - chr7_2:22529932-22529951 None:intergenic 20.0%
!!! AAATATATTTTTTGCAGTTG+TGG + chr7_2:22529935-22529954 Msa1053160:intron 20.0%
!!! TGGTTAAAGAAATAGTTTTA+AGG - chr7_2:22530118-22530137 None:intergenic 20.0%
!! ACCATGATAGTTATTTATAT+AGG + chr7_2:22530134-22530153 Msa1053160:intron 20.0%
!! CCATGATAGTTATTTATATA+GGG + chr7_2:22530135-22530154 Msa1053160:intron 20.0%
!! CAAAATATCTGTAATAGTTA+AGG - chr7_2:22530318-22530337 None:intergenic 20.0%
!!! CTTAACTATTACAGATATTT+TGG + chr7_2:22530316-22530335 Msa1053160:intron 20.0%
!!! AATCTTTCTTTTTCACTTCA+CGG + chr7_2:22527310-22527329 Msa1053160:five_prime_UTR 25.0%
! ACAGATCATTCAACATTTAT+TGG + chr7_2:22527392-22527411 Msa1053160:five_prime_UTR 25.0%
!!! AATGTTGGTTTGATACAAAA+TGG + chr7_2:22527635-22527654 Msa1053160:intron 25.0%
!!! GTTAAACATTTTAACCTTCA+AGG - chr7_2:22527816-22527835 None:intergenic 25.0%
! TATAAGTGTGTATGAAATGA+AGG + chr7_2:22528112-22528131 Msa1053160:intron 25.0%
!!! TTGTAATTGTTTCTACTAAC+TGG - chr7_2:22528481-22528500 None:intergenic 25.0%
! ATTAAGACTTCTTTCTGAAT+TGG + chr7_2:22528624-22528643 Msa1053160:intron 25.0%
! AATATGACATGTCTTTAAGT+CGG + chr7_2:22528912-22528931 Msa1053160:intron 25.0%
!! GAATGTATTGTTTTCAGATT+CGG - chr7_2:22528983-22529002 None:intergenic 25.0%
! TTTAACATAACTAGCGTATT+TGG + chr7_2:22529067-22529086 Msa1053160:intron 25.0%
! CAGTTTCAACAATTCTTTAT+TGG - chr7_2:22529141-22529160 None:intergenic 25.0%
! TGCTATTGAGAATTCAAATT+AGG + chr7_2:22529359-22529378 Msa1053160:intron 25.0%
!!! ACCTTTACAATGTTACTTTT+GGG + chr7_2:22529988-22530007 Msa1053160:CDS 25.0%
! TGTAGAAGAAAGTGAAATAA+AGG - chr7_2:22530055-22530074 None:intergenic 25.0%
! CCCTATATAAATAACTATCA+TGG - chr7_2:22530138-22530157 None:intergenic 25.0%
!! ATCTTTCTTTTTCACTTCAC+GGG + chr7_2:22527311-22527330 Msa1053160:five_prime_UTR 30.0%
!! ATCAACTGATTTTCTAACGA+AGG + chr7_2:22527584-22527603 Msa1053160:intron 30.0%
!! TCAACTGATTTTCTAACGAA+GGG + chr7_2:22527585-22527604 Msa1053160:intron 30.0%
AAAATTAAAGTGATCGCAGT+GGG - chr7_2:22527719-22527738 None:intergenic 30.0%
AAAAATTAAAGTGATCGCAG+TGG - chr7_2:22527720-22527739 None:intergenic 30.0%
TCAAAATGTTCTAACCTTGA+AGG + chr7_2:22527799-22527818 Msa1053160:intron 30.0%
!! AAACAGAAGTGTTTGTATGT+AGG + chr7_2:22528052-22528071 Msa1053160:intron 30.0%
! GAAATGAAGGCATATATGTT+TGG + chr7_2:22528125-22528144 Msa1053160:intron 30.0%
TGTCTTAATAAGTGCATGTT+TGG - chr7_2:22528288-22528307 None:intergenic 30.0%
AGAAAATGATACGTTTCTGT+CGG + chr7_2:22528376-22528395 Msa1053160:intron 30.0%
GAAACAATTACAAGCATCAA+AGG + chr7_2:22528488-22528507 Msa1053160:intron 30.0%
TGAATTGGTATTAACTTAGC+TGG + chr7_2:22528639-22528658 Msa1053160:intron 30.0%
AAAACATTATTCCACTCGTT+AGG - chr7_2:22528780-22528799 None:intergenic 30.0%
TAATAGCGATTCATATACTC+CGG + chr7_2:22528829-22528848 Msa1053160:intron 30.0%
ATATGACATGTCTTTAAGTC+GGG + chr7_2:22528913-22528932 Msa1053160:intron 30.0%
AACAATACATTCAAGAGCTT+AGG + chr7_2:22528991-22529010 Msa1053160:intron 30.0%
GATCCATAATTTGATTGAAG+CGG + chr7_2:22529115-22529134 Msa1053160:intron 30.0%
! ACATCAAATGTGCTTAGTTT+TGG + chr7_2:22529288-22529307 Msa1053160:intron 30.0%
!!! ATGGTTTTTTTCCATTAGTC+AGG - chr7_2:22529533-22529552 None:intergenic 30.0%
TGACGAATGAGGAATAATTA+TGG - chr7_2:22529552-22529571 None:intergenic 30.0%
TTGTATCCATCATTAATGCA+TGG + chr7_2:22529751-22529770 Msa1053160:intron 30.0%
CAAAAGTAACATTGTAAAGG+TGG - chr7_2:22529989-22530008 None:intergenic 30.0%
!!! CACCTTTACAATGTTACTTT+TGG + chr7_2:22529987-22530006 Msa1053160:CDS 30.0%
!!! TAGTTTTAAGGTAACTACGA+AGG - chr7_2:22530106-22530125 None:intergenic 30.0%
!! TCTTTCTTTTTCACTTCACG+GGG + chr7_2:22527312-22527331 Msa1053160:five_prime_UTR 35.0%
CCATATGATTGTTTCAGTCA+GGG - chr7_2:22527919-22527938 None:intergenic 35.0%
ATCCTCCAATATCTAGGAAA+GGG - chr7_2:22527986-22528005 None:intergenic 35.0%
AATCCTCCAATATCTAGGAA+AGG - chr7_2:22527987-22528006 None:intergenic 35.0%
GTCTTAATCCTCCAATATCT+AGG - chr7_2:22527992-22528011 None:intergenic 35.0%
AACCCAACGTAAACAATGAT+TGG + chr7_2:22528177-22528196 Msa1053160:intron 35.0%
CTCCAATCATTGTTTACGTT+GGG - chr7_2:22528182-22528201 None:intergenic 35.0%
ACTGAACGAAAGGTCAAAAT+AGG - chr7_2:22528261-22528280 None:intergenic 35.0%
TAGCTGGTTACATGGATAAT+GGG - chr7_2:22528337-22528356 None:intergenic 35.0%
TTCTTTACTAGCTGGTTACA+TGG - chr7_2:22528345-22528364 None:intergenic 35.0%
TTCAACGTTTCTTTACTAGC+TGG - chr7_2:22528353-22528372 None:intergenic 35.0%
TATTATCTCCAAAGTTGTCC+TGG + chr7_2:22528571-22528590 Msa1053160:intron 35.0%
TAGGCATAAGCTGTAAAGTA+TGG - chr7_2:22528761-22528780 None:intergenic 35.0%
ACATTCAAGAGCTTAGGATT+TGG + chr7_2:22528997-22529016 Msa1053160:intron 35.0%
!! GAGCAATGATTTGTTTGTGT+TGG + chr7_2:22529160-22529179 Msa1053160:intron 35.0%
CAATGCCACATCAAATAAGA+GGG - chr7_2:22529186-22529205 None:intergenic 35.0%
ACAATGCCACATCAAATAAG+AGG - chr7_2:22529187-22529206 None:intergenic 35.0%
AATTATTCCTCATTCGTCAG+TGG + chr7_2:22529553-22529572 Msa1053160:intron 35.0%
ATTATTCCTCATTCGTCAGT+GGG + chr7_2:22529554-22529573 Msa1053160:intron 35.0%
AGTTGTGGAAGTCATGAATT+GGG + chr7_2:22529950-22529969 Msa1053160:intron 35.0%
GCCCAAAAGTAACATTGTAA+AGG - chr7_2:22529992-22530011 None:intergenic 35.0%
!! TACTTTTGGGCTTTATATGC+TGG + chr7_2:22530001-22530020 Msa1053160:CDS 35.0%
ACGAAGGTAAAGACATCTTA+AGG - chr7_2:22530090-22530109 None:intergenic 35.0%
! TGCTAATGTGTTTTGTAGTG+CGG + chr7_2:22530345-22530364 Msa1053160:CDS 35.0%
ACATTACCCAATTCTTCAAG+AGG + chr7_2:22530369-22530388 Msa1053160:CDS 35.0%
GAACTACCTCTTGAAGAATT+GGG - chr7_2:22530378-22530397 None:intergenic 35.0%
TGAACTACCTCTTGAAGAAT+TGG - chr7_2:22530379-22530398 None:intergenic 35.0%
GATACAAAATGGTCGCATAG+CGG + chr7_2:22527646-22527665 Msa1053160:intron 40.0%
CCCTGACTGAAACAATCATA+TGG + chr7_2:22527916-22527935 Msa1053160:intron 40.0%
GCCATATGATTGTTTCAGTC+AGG - chr7_2:22527920-22527939 None:intergenic 40.0%
TCCTCCAATATCTAGGAAAG+GGG - chr7_2:22527985-22528004 None:intergenic 40.0%
CCTCCAATCATTGTTTACGT+TGG - chr7_2:22528183-22528202 None:intergenic 40.0%
CCAACGTAAACAATGATTGG+AGG + chr7_2:22528180-22528199 Msa1053160:intron 40.0%
!! ACATGGATAATGGGAGAATG+AGG - chr7_2:22528328-22528347 None:intergenic 40.0%
CTAGCTGGTTACATGGATAA+TGG - chr7_2:22528338-22528357 None:intergenic 40.0%
TACACAATCCAGGACAACTT+TGG - chr7_2:22528582-22528601 None:intergenic 40.0%
AGCGATCTTATACACAATCC+AGG - chr7_2:22528592-22528611 None:intergenic 40.0%
TTTCAGATTCGGATGCATGA+TGG - chr7_2:22528972-22528991 None:intergenic 40.0%
TGGTTTATGTCAAATCAGCG+AGG + chr7_2:22529087-22529106 Msa1053160:intron 40.0%
TGGCCGCTTCAATCAAATTA+TGG - chr7_2:22529121-22529140 None:intergenic 40.0%
AGTGTGAGGAAAACAATGTC+TGG + chr7_2:22529322-22529341 Msa1053160:intron 40.0%
! TGATTTGCGATTCACTTAGC+TGG + chr7_2:22529391-22529410 Msa1053160:intron 40.0%
CAGGCATAAGCTGTAAAGTA+TGG - chr7_2:22529514-22529533 None:intergenic 40.0%
TACAGCTTATGCCTGACTAA+TGG + chr7_2:22529519-22529538 Msa1053160:intron 40.0%
AGTCTACCACAGATGGTTAT+TGG + chr7_2:22529627-22529646 Msa1053160:intron 40.0%
CTCAAGCCATGCATTAATGA+TGG - chr7_2:22529760-22529779 None:intergenic 40.0%
CAGTTGTGGAAGTCATGAAT+TGG + chr7_2:22529949-22529968 Msa1053160:intron 40.0%
CATGAATTGGGTTCAGCAAA+AGG + chr7_2:22529962-22529981 Msa1053160:exon 40.0%
! TTGGGCTTTATATGCTGGAT+TGG + chr7_2:22530006-22530025 Msa1053160:CDS 40.0%
!! GCTTTATATGCTGGATTGGT+GGG + chr7_2:22530010-22530029 Msa1053160:CDS 40.0%
GCGTTAGACTCCGAATCTAA+AGG + chr7_2:22527679-22527698 Msa1053160:intron 45.0%
ACTCCGAATCTAAAGGTCGT+GGG + chr7_2:22527686-22527705 Msa1053160:intron 45.0%
GAACCCACGACCTTTAGATT+CGG - chr7_2:22527692-22527711 None:intergenic 45.0%
! GGAAAGGGGCTCTAGTAATT+TGG - chr7_2:22527971-22527990 None:intergenic 45.0%
AGAGCCCCTTTCCTAGATAT+TGG + chr7_2:22527978-22527997 Msa1053160:intron 45.0%
GTTTGGATGCACTGAACGAA+AGG - chr7_2:22528271-22528290 None:intergenic 45.0%
ATTTAAGCGAGCTGCAGCTT+AGG - chr7_2:22528726-22528745 None:intergenic 45.0%
TACAGCTTATGCCTAACGAG+TGG + chr7_2:22528766-22528785 Msa1053160:intron 45.0%
AGTCTGCCACAGATGGTTAT+TGG + chr7_2:22528868-22528887 Msa1053160:intron 45.0%
GACACACCAATAACCATCTG+TGG - chr7_2:22528877-22528896 None:intergenic 45.0%
! GTTGGCCCTCTTATTTGATG+TGG + chr7_2:22529178-22529197 Msa1053160:intron 45.0%
TGATGTGGCATTGTCTTGAC+TGG + chr7_2:22529193-22529212 Msa1053160:intron 45.0%
!! TGGAGTTCAAGTTGAGTGTG+AGG + chr7_2:22529308-22529327 Msa1053160:intron 45.0%
CAAATACCCACTGACGAATG+AGG - chr7_2:22529563-22529582 None:intergenic 45.0%
GGCAATAGTCTCGGAGTATA+TGG - chr7_2:22529601-22529620 None:intergenic 45.0%
ACTTGCTCAGGCAATAGTCT+CGG - chr7_2:22529610-22529629 None:intergenic 45.0%
GACACACCAATAACCATCTG+TGG - chr7_2:22528877-22528896 None:intergenic 45.0%
! GGCTTTATATGCTGGATTGG+TGG + chr7_2:22530009-22530028 Msa1053160:CDS 45.0%
AAATCTCATGCGATCGCACC+AGG + chr7_2:22527422-22527441 Msa1053160:five_prime_UTR 50.0%
! AAAATGGTCGCATAGCGGAG+TGG + chr7_2:22527651-22527670 Msa1053160:intron 50.0%
GACTCCGAATCTAAAGGTCG+TGG + chr7_2:22527685-22527704 Msa1053160:intron 50.0%
GCCCCTTTCCTAGATATTGG+AGG + chr7_2:22527981-22528000 Msa1053160:intron 50.0%
! ACTTGCTAAGGCAACAGAGC+CGG - chr7_2:22528851-22528870 None:intergenic 50.0%
!! CATCTGTGGCAGACTTGCTA+AGG - chr7_2:22528863-22528882 None:intergenic 50.0%
CTTAGCAAGTCTGCCACAGA+TGG + chr7_2:22528861-22528880 Msa1053160:intron 50.0%
! CATCTGTGGTAGACTTGCTC+AGG - chr7_2:22529622-22529641 None:intergenic 50.0%
CTGAGCAAGTCTACCACAGA+TGG + chr7_2:22529620-22529639 Msa1053160:intron 50.0%
CGCGTGCGTGCGTGCGTACC+TGG - chr7_2:22527443-22527462 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
chr7_2 gene 22527238 22530414 22527238 ID=Msa1053160;Name=Msa1053160
chr7_2 mRNA 22527238 22530414 22527238 ID=Msa1053160-mRNA-1;Parent=Msa1053160;Name=Msa1053160-mRNA-1;_AED=0.34;_eAED=0.34;_QI=217|1|0.66|1|0|0|3|20|49
chr7_2 exon 22527238 22527443 22527238 ID=Msa1053160-mRNA-1:exon:5085;Parent=Msa1053160-mRNA-1
chr7_2 exon 22529952 22530047 22529952 ID=Msa1053160-mRNA-1:exon:5086;Parent=Msa1053160-mRNA-1
chr7_2 exon 22530330 22530414 22530330 ID=Msa1053160-mRNA-1:exon:5087;Parent=Msa1053160-mRNA-1
chr7_2 five_prime_UTR 22527238 22527443 22527238 ID=Msa1053160-mRNA-1:five_prime_utr;Parent=Msa1053160-mRNA-1
chr7_2 five_prime_UTR 22529952 22529962 22529952 ID=Msa1053160-mRNA-1:five_prime_utr;Parent=Msa1053160-mRNA-1
chr7_2 CDS 22529963 22530047 22529963 ID=Msa1053160-mRNA-1:cds;Parent=Msa1053160-mRNA-1
chr7_2 CDS 22530330 22530394 22530330 ID=Msa1053160-mRNA-1:cds;Parent=Msa1053160-mRNA-1
chr7_2 three_prime_UTR 22530395 22530414 22530395 ID=Msa1053160-mRNA-1:three_prime_utr;Parent=Msa1053160-mRNA-1
Gene Sequence

>Msa1053160

ATGAATTGGGTTCAGCAAAAGGTCCACCTTTACAATGTTACTTTTGGGCTTTATATGCTGGATTGGTGGGAGCGTTGTTCATTCAATATTTTGGTGATTGTGCTAATGTGTTTTGTAGTGCGGTACATTACCCAATTCTTCAAGAGGTAG

Protein sequence

>Msa1053160

MNWVQQKVHLYNVTFGLYMLDWWERCSFNILVIVLMCFVVRYITQFFKR*