Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0011720 | Msa1066440 | 0.815063 | 1.119953e-51 | -8.615850e-47 |
| Msa0053900 | Msa1066440 | 0.804951 | 1.688379e-49 | -8.615850e-47 |
| Msa0060600 | Msa1066440 | 0.820253 | 7.561141e-53 | -8.615850e-47 |
| Msa0105340 | Msa1066440 | 0.811430 | 7.028726e-51 | -8.615850e-47 |
| Msa0611550 | Msa1066440 | 0.802840 | 4.634553e-49 | -8.615850e-47 |
| Msa0622340 | Msa1066440 | 0.800761 | 1.238582e-48 | -8.615850e-47 |
| Msa0651790 | Msa1066440 | 0.801672 | 8.063113e-49 | -8.615850e-47 |
| Msa0694250 | Msa1066440 | 0.810639 | 1.042963e-50 | -8.615850e-47 |
| Msa0724050 | Msa1066440 | 0.808919 | 2.444880e-50 | -8.615850e-47 |
| Msa0763880 | Msa1066440 | 0.802672 | 5.019175e-49 | -8.615850e-47 |
| Msa0148340 | Msa1066440 | 0.819764 | 9.786616e-53 | -8.615850e-47 |
| Msa0148360 | Msa1066440 | 0.811698 | 6.147455e-51 | -8.615850e-47 |
| Msa0408630 | Msa1066440 | 0.813433 | 2.566431e-51 | -8.615850e-47 |
| Msa0419620 | Msa1066440 | -0.811827 | 5.760692e-51 | -8.615850e-47 |
| Msa0426330 | Msa1066440 | 0.805647 | 1.206548e-49 | -8.615850e-47 |
| Msa0999040 | Msa1066440 | 0.801398 | 9.174505e-49 | -8.615850e-47 |
| Msa1066430 | Msa1066440 | 0.819570 | 1.083350e-52 | -8.615850e-47 |
| Msa1066440 | Msa1142500 | 0.805881 | 1.077714e-49 | -8.615850e-47 |
| Msa1066440 | Msa1218650 | 0.800522 | 1.385454e-48 | -8.615850e-47 |
| Msa1066440 | Msa1419110 | 0.809973 | 1.451823e-50 | -8.615850e-47 |
| Msa1066440 | Msa1423450 | 0.815656 | 8.269194e-52 | -8.615850e-47 |
| Msa1066440 | Msa1439230 | 0.802525 | 5.383016e-49 | -8.615850e-47 |
| Msa0252180 | Msa1066440 | 0.814830 | 1.261768e-51 | -8.615850e-47 |
| Msa0556240 | Msa1066440 | 0.801326 | 9.490476e-49 | -8.615850e-47 |
| Msa0870620 | Msa1066440 | 0.813243 | 2.825093e-51 | -8.615850e-47 |
| Msa0908170 | Msa1066440 | 0.800957 | 1.129139e-48 | -8.615850e-47 |
| Msa0937240 | Msa1066440 | 0.804231 | 2.385423e-49 | -8.615850e-47 |
| Msa0944770 | Msa1066440 | 0.812592 | 3.922649e-51 | -8.615850e-47 |
| Msa0946320 | Msa1066440 | 0.808937 | 2.422993e-50 | -8.615850e-47 |
| Msa0974530 | Msa1066440 | 0.817660 | 2.938848e-52 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 10 sgRNAs with CRISPR-Local
Find 33 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATTGTATCAACATCCTTAT+TGG | 0.184494 | 7_2:+60477735 | Msa1066440:CDS |
| ACTTGAAATCATGGCCAATA+AGG | 0.364486 | 7_2:-60477749 | None:intergenic |
| CTACATTGAACTTGAAATCA+TGG | 0.442344 | 7_2:-60477758 | None:intergenic |
| ACAACAGAATATAGACATCT+TGG | 0.464268 | 7_2:-60477805 | None:intergenic |
| CAATCCAACAAAAGAAACAT+TGG | 0.519243 | 7_2:-60477852 | None:intergenic |
| TTGTTGGATTGTCCAACATG+AGG | 0.529572 | 7_2:+60477864 | Msa1066440:CDS |
| TGACAATTCAAACATGGATG+AGG | 0.556876 | 7_2:+60477180 | None:intergenic |
| TACATGGACAACCCTCATGT+TGG | 0.566264 | 7_2:-60477876 | None:intergenic |
| TGTTGGATTGTCCAACATGA+GGG | 0.570912 | 7_2:+60477865 | Msa1066440:CDS |
| GGACACGTTTCAACTTGTTG+TGG | 0.622647 | 7_2:-60477831 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TATAAGTAACTTGTTAAATT+TGG | + | chr7_2:60477320-60477339 | Msa1066440:intron | 15.0% |
| !! | AATCTTACAATCATATTAAA+AGG | - | chr7_2:60477356-60477375 | None:intergenic | 15.0% |
| !! | TTAACAAAAAATAGTTTCAA+AGG | + | chr7_2:60477374-60477393 | Msa1066440:intron | 15.0% |
| !!! | ATCATTAGTATTGTTATTAT+TGG | + | chr7_2:60477468-60477487 | Msa1066440:intron | 15.0% |
| !!! | TCATTAGTATTGTTATTATT+GGG | + | chr7_2:60477469-60477488 | Msa1066440:intron | 15.0% |
| !!! | ATCATTGTTCATTTGTTAAT+AGG | + | chr7_2:60477276-60477295 | Msa1066440:intron | 20.0% |
| !! | TTTCAAAGGATTATATTTGT+TGG | + | chr7_2:60477388-60477407 | Msa1066440:intron | 20.0% |
| !! | GATGTCCAATTTATTATAAT+TGG | - | chr7_2:60477556-60477575 | None:intergenic | 20.0% |
| ! | TATTATTGGGACAAATTGAA+CGG | + | chr7_2:60477482-60477501 | Msa1066440:intron | 25.0% |
| ! | TTTATTATAATTGGCAGCTA+TGG | - | chr7_2:60477547-60477566 | None:intergenic | 25.0% |
| ! | AGCTGCCAATTATAATAAAT+TGG | + | chr7_2:60477548-60477567 | Msa1066440:intron | 25.0% |
| ! | ATAATAAATTGGACATCACA+TGG | + | chr7_2:60477559-60477578 | Msa1066440:intron | 25.0% |
| ! | ATAAAATGAGACAACATTGT+TGG | + | chr7_2:60477626-60477645 | Msa1066440:intron | 25.0% |
| !!! | AAAAGCACATTATTTTTCTG+TGG | - | chr7_2:60477672-60477691 | None:intergenic | 25.0% |
| !!! | ATACAATTTTGATATGTCGA+TGG | - | chr7_2:60477723-60477742 | None:intergenic | 25.0% |
| ! | AATTGTATCAACATCCTTAT+TGG | + | chr7_2:60477735-60477754 | Msa1066440:CDS | 25.0% |
| TTGGATTTCTCATAGATGTT+TGG | - | chr7_2:60477426-60477445 | None:intergenic | 30.0% | |
| ACATCTATGAGAAATCCAAA+TGG | + | chr7_2:60477427-60477446 | Msa1066440:intron | 30.0% | |
| CATCTATGAGAAATCCAAAT+GGG | + | chr7_2:60477428-60477447 | Msa1066440:intron | 30.0% | |
| GAGTAAATCAAATTCCCATT+TGG | - | chr7_2:60477445-60477464 | None:intergenic | 30.0% | |
| !!! | CTTTTTTTAGAACATGTGCT+TGG | + | chr7_2:60477687-60477706 | Msa1066440:intron | 30.0% |
| CTACATTGAACTTGAAATCA+TGG | - | chr7_2:60477761-60477780 | None:intergenic | 30.0% | |
| ACAACAGAATATAGACATCT+TGG | - | chr7_2:60477808-60477827 | None:intergenic | 30.0% | |
| CAATCCAACAAAAGAAACAT+TGG | - | chr7_2:60477855-60477874 | None:intergenic | 30.0% | |
| GGAGTAGTATCTATGTATCT+AGG | + | chr7_2:60477220-60477239 | Msa1066440:intron | 35.0% | |
| ATTGGACATCACATGGTAAT+TGG | + | chr7_2:60477566-60477585 | Msa1066440:intron | 35.0% | |
| ACTTGAAATCATGGCCAATA+AGG | - | chr7_2:60477752-60477771 | None:intergenic | 35.0% | |
| ! | GTGTCCAATGTTTCTTTTGT+TGG | + | chr7_2:60477848-60477867 | Msa1066440:CDS | 35.0% |
| ! | GAGGTGAGACAAGATTTTTG+TGG | + | chr7_2:60477199-60477218 | Msa1066440:intron | 40.0% |
| TCAATCATGTGTTCAGTCCA+TGG | + | chr7_2:60477521-60477540 | Msa1066440:intron | 40.0% | |
| !! | GGAAGCTGTCAAAGCATTTT+TGG | - | chr7_2:60477787-60477806 | None:intergenic | 40.0% |
| ATAATTGGCAGCTATGGCCA+TGG | - | chr7_2:60477541-60477560 | None:intergenic | 45.0% | |
| !! | GGACACGTTTCAACTTGTTG+TGG | - | chr7_2:60477834-60477853 | None:intergenic | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_2 | gene | 60477193 | 60477884 | 60477193 | ID=Msa1066440;Name=Msa1066440 |
| chr7_2 | mRNA | 60477193 | 60477884 | 60477193 | ID=Msa1066440-mRNA-1;Parent=Msa1066440;Name=Msa1066440-mRNA-1;_AED=0.97;_eAED=1.00;_QI=0|0|0|0.5|1|1|2|0|59 |
| chr7_2 | exon | 60477193 | 60477201 | 60477193 | ID=Msa1066440-mRNA-1:exon:11869;Parent=Msa1066440-mRNA-1 |
| chr7_2 | exon | 60477714 | 60477884 | 60477714 | ID=Msa1066440-mRNA-1:exon:11870;Parent=Msa1066440-mRNA-1 |
| chr7_2 | CDS | 60477193 | 60477201 | 60477193 | ID=Msa1066440-mRNA-1:cds;Parent=Msa1066440-mRNA-1 |
| chr7_2 | CDS | 60477714 | 60477884 | 60477714 | ID=Msa1066440-mRNA-1:cds;Parent=Msa1066440-mRNA-1 |
| Gene Sequence |
| Protein sequence |
