AlfalfaGEDB | Msa1142500

M. sativa cultivar zhongmu-4 / Msa1142500

KEGG: -
GO: -
PFAM: PF13302.10

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1142500	KAG5626294.1	44.615	325	142	7	3	290	161	484	1.44e-82	273
Msa1142500	KAG5626294.1	44.877	488	219	8	4	443	1	486	3.18e-141	423

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1142500	sp\|P05332\|YP20_BACLI	32.530	166	104	3	279	437	8	172	1.16e-15	78.2

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1142500	A0A7N2LTV9	43.067	476	226	9	4	441	7	475	5.90e-122	373

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TFs/TRs:

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Gene ID	Type	Classification
Msa1142500	TR	GNAT

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0005350	Msa1142500	0.844895	5.693191e-59	-8.615850e-47
Msa0006700	Msa1142500	0.835948	1.246670e-56	-8.615850e-47
Msa0011720	Msa1142500	0.843095	1.730188e-58	-8.615850e-47
Msa0014800	Msa1142500	0.834490	2.908401e-56	-8.615850e-47
Msa0020630	Msa1142500	0.818916	1.527089e-52	-8.615850e-47
Msa0043940	Msa1142500	0.812593	3.920794e-51	-8.615850e-47
Msa0044710	Msa1142500	0.839981	1.144521e-57	-8.615850e-47
Msa0050720	Msa1142500	0.811757	5.966584e-51	-8.615850e-47
Msa0050730	Msa1142500	0.825231	5.246178e-54	-8.615850e-47
Msa0050760	Msa1142500	0.831369	1.736249e-55	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1142500	MtrunA17_Chr7g0223351	93.750	176	11	0	272	447	1	176	4.41e-119	345
Msa1142500	MtrunA17_Chr7g0223351	83.562	146	14	1	135	270	4	149	2.78e-80	246
Msa1142500	MtrunA17_Chr7g0223351	66.667	144	38	1	1	134	1	144	4.23e-61	196
Msa1142500	MtrunA17_Chr7g0223361	69.364	173	52	1	272	443	1	173	1.36e-80	246
Msa1142500	MtrunA17_Chr7g0223361	82.069	145	15	1	1	134	1	145	2.66e-79	243
Msa1142500	MtrunA17_Chr7g0223361	66.207	145	38	1	138	271	7	151	1.21e-63	203
Msa1142500	MtrunA17_Chr5g0394631	32.895	380	177	10	138	440	20	398	2.07e-57	194
Msa1142500	MtrunA17_Chr5g0394631	35.758	165	85	3	4	150	17	178	3.87e-24	103
Msa1142500	MtrunA17_Chr5g0394631	42.384	151	66	6	3	134	225	373	1.66e-23	102
Msa1142500	MtrunA17_Chr7g0273661	58.228	158	64	2	272	427	1	158	1.89e-55	181

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1142500	AT2G32020.1	40.805	174	99	3	271	442	11	182	2.44e-42	148
Msa1142500	AT2G32020.1	40.909	154	81	3	139	282	19	172	8.09e-36	130
Msa1142500	AT2G32020.1	42.361	144	73	3	1	134	12	155	7.93e-33	123
Msa1142500	AT3G22560.1	44.444	171	91	3	274	440	2	172	9.51e-41	144
Msa1142500	AT3G22560.1	43.836	146	68	3	3	134	2	147	8.22e-31	117
Msa1142500	AT3G22560.1	42.466	146	70	3	139	270	7	152	4.68e-29	112
Msa1142500	AT2G32030.1	40.361	166	95	3	279	442	24	187	3.38e-39	140
Msa1142500	AT2G32030.1	38.037	163	90	4	139	290	24	186	1.14e-31	120
Msa1142500	AT2G32030.1	39.568	139	74	3	6	134	22	160	1.20e-28	112

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Find 111 sgRNAs with CRISPR-Local

Find 762 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGTTGGCTTTCATAGGG+AGG	0.608005	7_4:-16940389	Msa1142500:CDS
AAAGGTAGGGTTCTACAGAG+AGG	0.693917	7_4:-16948998	Msa1142500:CDS
AAAGGTTGGTTTCCAAAGGG+AGG	0.574627	7_4:-16937449	Msa1142500:CDS
AAAGTTGGCTTTCATAGGGA+GGG	0.561963	7_4:-16940388	Msa1142500:CDS
AAGAATCCAAACCATTCCAA+TGG	0.587139	7_4:+16949209	None:intergenic
AAGAATCTTCACTGCATTAG+TGG	0.453856	7_4:+16949110	None:intergenic
AAGACTTTCCTGATTTGGTA+AGG	0.487902	7_4:-16937522	Msa1142500:CDS
AAGAGAACGTAGCTTCTCAG+AGG	0.683092	7_4:-16949029	Msa1142500:CDS
AAGAGTCAAGGATCACTCTC+CGG	0.583353	7_4:-16937846	Msa1142500:CDS
AAGGGAGGGATTGTTCAGAA+AGG	0.588499	7_4:-16937434	Msa1142500:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TGGCAATAATAAATCCATCA+AGG	-	chr7_4:16937352-16937371	Msa1142500:CDS	30.0%
AGTAACTAAGCATACTAGAT+GGG	-	chr7_4:16937440-16937459	Msa1142500:CDS	30.0%
ATGCTATTGGATTCAACTAT+TGG	-	chr7_4:16937616-16937635	Msa1142500:CDS	30.0%
TGCTATTGGATTCAACTATT+GGG	-	chr7_4:16937617-16937636	Msa1142500:CDS	30.0%
GATCTCTCTCATTTATAAAG+AGG	+	chr7_4:16937953-16937972	None:intergenic	30.0%
GACAATAAGTATGAGTAACT+AGG	-	chr7_4:16938239-16938258	Msa1142500:intron	30.0%
CAATAAGTATGAGTAACTAG+GGG	-	chr7_4:16938241-16938260	Msa1142500:intron	30.0%
AATAAGTATGAGTAACTAGG+GGG	-	chr7_4:16938242-16938261	Msa1142500:intron	30.0%
TGTGCTTTACTATATAAGTG+TGG	+	chr7_4:16938460-16938479	None:intergenic	30.0%
AATTGTAACCAACTATGTGA+TGG	-	chr7_4:16938479-16938498	Msa1142500:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr7_4	gene	16937351	16949433	16937351	ID=Msa1142500;Name=Msa1142500
chr7_4	mRNA	16937351	16949433	16937351	ID=Msa1142500-mRNA-1;Parent=Msa1142500;Name=Msa1142500-mRNA-1;_AED=0.20;_eAED=0.20;_QI=0\|0\|0\|1\|0.25\|0.2\|5\|0\|447
chr7_4	exon	16949221	16949433	16949221	ID=Msa1142500-mRNA-1:exon:3812;Parent=Msa1142500-mRNA-1
chr7_4	exon	16948998	16949187	16948998	ID=Msa1142500-mRNA-1:exon:3811;Parent=Msa1142500-mRNA-1
chr7_4	exon	16940609	16940811	16940609	ID=Msa1142500-mRNA-1:exon:3810;Parent=Msa1142500-mRNA-1
chr7_4	exon	16940375	16940578	16940375	ID=Msa1142500-mRNA-1:exon:3809;Parent=Msa1142500-mRNA-1
chr7_4	exon	16937351	16937884	16937351	ID=Msa1142500-mRNA-1:exon:3808;Parent=Msa1142500-mRNA-1
chr7_4	CDS	16949221	16949433	16949221	ID=Msa1142500-mRNA-1:cds;Parent=Msa1142500-mRNA-1
chr7_4	CDS	16948998	16949187	16948998	ID=Msa1142500-mRNA-1:cds;Parent=Msa1142500-mRNA-1
chr7_4	CDS	16940609	16940811	16940609	ID=Msa1142500-mRNA-1:cds;Parent=Msa1142500-mRNA-1
chr7_4	CDS	16940375	16940578	16940375	ID=Msa1142500-mRNA-1:cds;Parent=Msa1142500-mRNA-1
chr7_4	CDS	16937351	16937884	16937351	ID=Msa1142500-mRNA-1:cds;Parent=Msa1142500-mRNA-1

Gene Sequence

>Msa1142500

ATGGCAATAATAAATCCATCAAGGATCACTATTCGACCATTCAAGGAAAGTGACGTAGATGATGTGTTATTATGGCTAGGAGATGAAAGAGTAACTAAGCATACTAGATGGGAAACATGTAATTCAAAGAAAGAAGCTTTGGATTTCATAAAATATGAATGCATTTACCCATTACGCCAATCAATATGTCTTGATGACCACTCCATTGGAATGTATAAAGCAGATTTTGGTTATGCTATTGGATTCAACTATTGGGGTCAAGGGATTGCCACTAATGCAGTGAAGATTCTTCTGTCAAAAGTGTTCCATGAGTTCCCCGATTTGAGAAGGTTGCAAGCTTTCACTGTTAAAGAGAACGTAGCTTCTCAGAGGGTGTTGGAAAAGGTAGGGTTCTACAGAGAGGATCATGAGTCAAGGATCACTCTTCGGCCATTCAAAGAAAGTGATGTTGACGATGTGTTGTTGTGGGCAAGTGATGAAAGAGTCATGCAATATACACGACTGGAGTTATGTAAATCAAGGGAACAAGCTTTGAATTTCATAAAAAAAGATTGTATTTACCCATTCCAACAATCAATATGTCTTGATGATCACTCCATTGGAATTTTTAAAGCAGATTTGGGTTTTGCTATTGGGTTCAATTATTGGGGCCAAGGTATAGCCACAAAAGCACTTAAGATTCTCTTGTCTCAAGTGTTCCAAGAGTTTTCTGACTTGCAAAGGTTGCAGGCTAATATTCTTGTAGACAACATGGCTTCACAAAGGCTGTTGGAAAAAGTTGGCTTTCATAGGGAGGGATTGTTCAGAAAGGTCATGGCAATAGATCAAGAGTCAAGGATCACTCTCCGGCCATTCAAAGAAAGTGATGCTGACGATGTGTTGTTGTGGGCAAGTGATGAAAGAGTCATGCAATATATGCGATTGGAATTATGTAAATCAAGGGAACAAGCTTTGAATTTCATAAAAAATGAATGCATTTACCCATTCCAACAATCAATATGTCTTAATGATCACTCTATTGGAATTGTTTTTGTCCTTCCTTATCCTGATGCAAAGTTTAAAGCAGATTTAGGCTATGCTATTGGGTTCAACTATTGGGGCCAAGGTGTAGCTACCAAAGCAGTGAAGATTCTTTTGTCTTGTGTATTCCAAGACTTTCCTGATTTGGTAAGGTTGCAGGCTAATACTCGTCCAGAGAACATGGCTTCTCAAAGACTGTTGGAAAAGGTTGGTTTCCAAAGGGAGGGATTGTTCAGAAAGGGAATTTATAACAAAGGGAATGTAGAGGATTTCTGTATTTTTAGTTTCTTGTCTACCGATGAAATTCCTCATGCTAAGGGTTAA

Protein sequence

>Msa1142500

MAIINPSRITIRPFKESDVDDVLLWLGDERVTKHTRWETCNSKKEALDFIKYECIYPLRQSICLDDHSIGMYKADFGYAIGFNYWGQGIATNAVKILLSKVFHEFPDLRRLQAFTVKENVASQRVLEKVGFYREDHESRITLRPFKESDVDDVLLWASDERVMQYTRLELCKSREQALNFIKKDCIYPFQQSICLDDHSIGIFKADLGFAIGFNYWGQGIATKALKILLSQVFQEFSDLQRLQANILVDNMASQRLLEKVGFHREGLFRKVMAIDQESRITLRPFKESDADDVLLWASDERVMQYMRLELCKSREQALNFIKNECIYPFQQSICLNDHSIGIVFVLPYPDAKFKADLGYAIGFNYWGQGVATKAVKILLSCVFQDFPDLVRLQANTRPENMASQRLLEKVGFQREGLFRKGIYNKGNVEDFCIFSFLSTDEIPHAKG*