AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0651790

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0651790 MtrunA17_Chr4g0044961 98.469 196 3 0 1 196 1 196 6.23e-143 395
Msa0651790 MtrunA17_Chr5g0402331 46.114 193 89 6 1 189 1 182 1.93e-46 151
Msa0651790 MtrunA17_Chr5g0402361 51.316 152 68 3 42 189 205 354 4.54e-44 150
Msa0651790 MtrunA17_Chr5g0402361 45.263 190 94 3 1 189 1 181 8.33e-44 149
Msa0651790 MtrunA17_Chr5g0402361 30.675 163 83 2 56 188 119 281 2.76e-13 67.4
Msa0651790 MtrunA17_Chr1g0161211 42.446 139 78 1 56 192 45 183 1.46e-31 113
Msa0651790 MtrunA17_Chr8g0391691 43.262 141 78 1 57 195 36 176 2.49e-31 112
Msa0651790 MtrunA17_Chr3g0111371 40.876 137 79 1 56 192 4 138 7.22e-31 109
Msa0651790 MtrunA17_Chr5g0434951 43.066 137 77 1 56 191 3 139 2.07e-30 108
Msa0651790 MtrunA17_Chr8g0392951 38.889 144 87 1 50 192 17 160 9.45e-29 105
Msa0651790 MtrunA17_Chr1g0169581 39.286 140 81 1 56 191 3 142 5.30e-27 100
Msa0651790 MtrunA17_Chr6g0482731 38.889 144 82 3 55 194 24 165 3.20e-25 96.3
Msa0651790 MtrunA17_Chr8g0351681 37.838 148 82 4 57 194 49 196 1.35e-24 95.5
Msa0651790 MtrunA17_Chr7g0256171 37.226 137 82 2 57 189 5 141 4.23e-23 90.1
Msa0651790 MtrunA17_Chr7g0245911 38.621 145 78 4 56 189 46 190 6.51e-23 90.9
Msa0651790 MtrunA17_Chr4g0033601 36.957 138 83 2 56 189 4 141 2.75e-22 88.2
Msa0651790 MtrunA17_Chr7g0254731 35.766 137 86 1 55 189 10 146 3.50e-22 87.8
Msa0651790 MtrunA17_Chr3g0116151 34.615 130 84 1 61 189 10 139 1.07e-21 86.3
Msa0651790 MtrunA17_Chr3g0116141 34.615 130 84 1 61 189 9 138 1.10e-21 86.3
Msa0651790 MtrunA17_Chr1g0169621 33.537 164 80 2 56 190 3 166 1.38e-21 86.7
Msa0651790 MtrunA17_Chr3g0093271 36.296 135 84 1 57 189 63 197 1.80e-21 87.4
Msa0651790 MtrunA17_Chr1g0188171 36.842 133 82 1 59 189 14 146 2.78e-21 85.5
Msa0651790 MtrunA17_Chr5g0440931 35.766 137 86 1 55 189 10 146 2.83e-21 85.5
Msa0651790 MtrunA17_Chr6g0460211 35.766 137 86 1 55 189 10 146 3.09e-21 85.5
Msa0651790 MtrunA17_Chr4g0057661 35.075 134 86 1 57 189 6 139 6.29e-21 84.3
Msa0651790 MtrunA17_Chr2g0329371 35.714 140 84 2 56 189 63 202 1.02e-20 85.5
Msa0651790 MtrunA17_Chr1g0166281 36.296 135 84 1 57 189 12 146 5.37e-20 82.0
Msa0651790 MtrunA17_Chr7g0225921 35.338 133 80 3 61 189 10 140 9.75e-20 81.3
Msa0651790 MtrunA17_Chr4g0057641 34.328 134 87 1 57 189 6 139 2.05e-19 80.5
Msa0651790 MtrunA17_Chr4g0057651 34.328 134 87 1 57 189 6 139 3.34e-19 79.7
Msa0651790 MtrunA17_Chr8g0366401 29.371 143 96 1 55 192 15 157 3.56e-19 80.5
Msa0651790 MtrunA17_Chr6g0459231 33.846 130 85 1 61 189 12 141 5.19e-19 79.3
Msa0651790 MtrunA17_Chr4g0062221 29.577 142 96 1 55 192 9 150 1.20e-18 79.3
Msa0651790 MtrunA17_Chr1g0185001 34.921 126 81 1 65 189 20 145 1.46e-18 78.2
Msa0651790 MtrunA17_Chr7g0256701 35.000 140 84 3 57 189 73 212 1.92e-18 79.7
Msa0651790 MtrunA17_Chr4g0064231 35.714 154 79 3 56 189 63 216 4.11e-18 79.0
Msa0651790 MtrunA17_Chr7g0245231 31.646 158 105 2 41 195 14 171 1.11e-17 76.6
Msa0651790 MtrunA17_Chr8g0373691 34.074 135 87 2 58 190 54 188 1.25e-16 74.3
Msa0651790 MtrunA17_Chr8g0368681 27.206 136 95 1 61 192 16 151 1.84e-16 73.2
Msa0651790 MtrunA17_Chr5g0396261 35.965 114 69 1 82 191 2 115 1.85e-16 72.0
Msa0651790 MtrunA17_Chr7g0274101 34.615 130 83 1 62 189 17 146 5.65e-16 71.6
Msa0651790 MtrunA17_Chr6g0450921 30.556 180 108 4 19 195 5 170 1.59e-15 70.9
Msa0651790 MtrunA17_Chr5g0402351 47.561 82 42 1 51 131 17 98 1.86e-15 69.3
Msa0651790 MtrunA17_Chr7g0245371 31.507 146 86 2 58 189 25 170 2.16e-15 70.9
Msa0651790 MtrunA17_Chr5g0441691 33.083 133 87 2 58 189 374 505 2.22e-15 73.6
Msa0651790 MtrunA17_Chr5g0409441 31.250 128 84 2 61 185 26 152 3.28e-15 69.7
Msa0651790 MtrunA17_Chr4g0074811 28.409 176 120 3 23 195 362 534 6.11e-15 72.4
Msa0651790 MtrunA17_Chr3g0131181 29.310 174 114 4 21 189 296 465 1.44e-14 71.2
Msa0651790 MtrunA17_Chr1g0160151 29.252 147 98 2 56 196 21 167 4.16e-14 67.0
Msa0651790 MtrunA17_Chr4g0059951 32.143 140 91 2 54 189 372 511 7.72e-14 69.3
Msa0651790 MtrunA17_Chr5g0397101 33.088 136 83 3 58 189 372 503 8.71e-14 68.9
Msa0651790 MtrunA17_Chr5g0444171 33.083 133 87 2 58 189 398 529 1.27e-13 68.6
Msa0651790 MtrunA17_Chr4g0074791 29.944 177 101 6 25 195 369 528 1.40e-13 68.6
Msa0651790 MtrunA17_Chr1g0157131 29.139 151 97 4 46 189 364 511 1.44e-13 68.6
Msa0651790 MtrunA17_Chr1g0176751 32.836 134 85 3 58 189 430 560 2.04e-13 68.2
Msa0651790 MtrunA17_Chr8g0387671 33.083 133 87 2 58 189 372 503 2.28e-13 67.8
Msa0651790 MtrunA17_Chr7g0267751 28.235 170 115 3 23 189 406 571 2.65e-13 67.8
Msa0651790 MtrunA17_Chr0c02g0489761 32.331 133 88 2 58 189 384 515 2.68e-13 67.8
Msa0651790 MtrunA17_Chr1g0170281 28.824 170 114 3 23 189 389 554 3.39e-13 67.4
Msa0651790 MtrunA17_Chr3g0121191 29.078 141 88 2 57 195 93 223 2.78e-12 63.2
Msa0651790 MtrunA17_Chr8g0385341 28.235 170 115 3 23 189 301 466 4.07e-12 64.3
Msa0651790 MtrunA17_Chr3g0098951 30.216 139 90 3 58 189 389 527 4.66e-12 63.9
Msa0651790 MtrunA17_Chr1g0200601 31.852 135 85 4 58 189 336 466 6.28e-12 63.5
Msa0651790 MtrunA17_Chr4g0042491 41.429 70 41 0 123 192 109 178 8.47e-11 58.5
Msa0651790 MtrunA17_Chr5g0448721 30.496 141 79 4 58 189 402 532 9.34e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0651790 AT2G15680.1 59.375 192 71 1 1 192 1 185 2.62e-80 237
Msa0651790 AT1G24620.1 44.286 140 76 1 57 194 37 176 3.08e-33 117
Msa0651790 AT1G73630.1 45.324 139 73 2 53 190 15 151 4.64e-32 114
Msa0651790 AT1G18210.2 42.222 135 76 1 56 190 22 154 1.51e-29 107
Msa0651790 AT1G18210.1 42.222 135 76 1 56 190 22 154 1.51e-29 107
Msa0651790 AT1G66400.1 40.141 142 77 2 54 189 12 151 6.34e-29 105
Msa0651790 AT5G37770.1 38.621 145 83 1 54 192 14 158 1.22e-27 102
Msa0651790 AT5G37770.2 38.621 145 83 1 54 192 14 158 1.22e-27 102
Msa0651790 AT3G03400.1 36.800 125 73 2 61 180 9 132 9.47e-25 94.4
Msa0651790 AT3G07490.1 38.462 143 72 3 57 189 5 141 1.66e-24 94.4
Msa0651790 AT5G17470.1 38.806 134 78 1 61 190 6 139 3.96e-24 93.2
Msa0651790 AT5G17470.1 49.123 57 29 0 58 114 78 134 4.00e-11 58.9
Msa0651790 AT5G42380.1 35.971 139 86 1 54 189 46 184 6.37e-24 93.6
Msa0651790 AT1G05990.1 38.406 138 80 2 57 189 5 142 3.98e-23 90.5
Msa0651790 AT4G12860.1 39.416 137 79 2 57 189 5 141 5.84e-23 90.1
Msa0651790 AT4G03290.1 37.857 140 80 2 57 189 5 144 4.16e-22 88.2
Msa0651790 AT2G36180.1 37.857 140 82 2 58 192 1 140 7.66e-22 87.0
Msa0651790 AT1G66410.1 35.766 137 86 1 55 189 10 146 1.56e-21 86.7
Msa0651790 AT5G37780.1 35.766 137 86 1 55 189 10 146 1.56e-21 86.7
Msa0651790 AT5G21274.1 36.496 137 85 1 55 189 10 146 1.69e-21 86.3
Msa0651790 AT3G43810.1 35.766 137 86 1 55 189 10 146 4.26e-21 85.5
Msa0651790 AT2G43290.1 33.562 146 86 3 53 189 63 206 5.28e-21 86.7
Msa0651790 AT3G43810.2 35.766 137 86 1 55 189 10 146 7.88e-21 86.3
Msa0651790 AT2G27030.3 34.507 142 91 1 55 194 10 151 1.06e-20 85.1
Msa0651790 AT3G56800.1 35.036 137 87 1 55 189 10 146 1.60e-20 84.0
Msa0651790 AT2G41110.1 35.036 137 87 1 55 189 10 146 1.60e-20 84.0
Msa0651790 AT2G27030.1 35.036 137 87 1 55 189 10 146 1.60e-20 84.0
Msa0651790 AT1G76650.1 35.075 134 86 1 57 189 43 176 6.64e-20 83.2
Msa0651790 AT1G76650.3 35.075 134 86 1 57 189 43 176 6.64e-20 83.2
Msa0651790 AT2G41100.7 32.278 158 88 1 55 193 64 221 1.83e-19 84.3
Msa0651790 AT2G41100.7 29.545 132 75 1 82 195 2 133 5.65e-13 66.2
Msa0651790 AT2G41100.6 32.278 158 88 1 55 193 64 221 1.83e-19 84.3
Msa0651790 AT2G41100.6 29.545 132 75 1 82 195 2 133 5.65e-13 66.2
Msa0651790 AT2G41100.3 32.278 158 88 1 55 193 64 221 1.83e-19 84.3
Msa0651790 AT2G41100.3 29.545 132 75 1 82 195 2 133 5.65e-13 66.2
Msa0651790 AT1G18530.1 29.286 140 95 1 57 192 7 146 2.94e-19 80.9
Msa0651790 AT2G41100.4 32.278 158 88 1 55 193 99 256 3.09e-19 84.0
Msa0651790 AT2G41100.4 30.189 159 93 1 55 195 10 168 5.15e-18 80.5
Msa0651790 AT2G41100.1 32.278 158 88 1 55 193 99 256 3.09e-19 84.0
Msa0651790 AT2G41100.1 30.189 159 93 1 55 195 10 168 5.15e-18 80.5
Msa0651790 AT2G41410.1 34.320 169 100 5 30 193 49 211 4.79e-19 81.6
Msa0651790 AT4G14640.1 34.815 135 86 1 57 189 13 147 9.39e-19 79.3
Msa0651790 AT3G10190.1 36.029 136 84 3 57 189 70 205 1.02e-18 80.5
Msa0651790 AT2G41090.1 34.815 135 87 1 55 189 10 143 1.05e-18 80.1
Msa0651790 AT3G22930.1 33.571 140 91 1 52 189 30 169 4.60e-18 78.2
Msa0651790 AT1G76640.1 32.051 156 89 3 44 189 10 158 4.77e-18 77.8
Msa0651790 AT5G37780.4 35.537 121 76 1 71 189 13 133 6.25e-18 76.6
Msa0651790 AT1G66410.2 35.537 121 76 1 71 189 36 156 6.62e-18 77.4
Msa0651790 AT5G37780.3 35.537 121 76 1 71 189 52 172 6.73e-18 77.8
Msa0651790 AT3G59440.1 33.099 142 80 3 57 189 51 186 7.13e-18 78.2
Msa0651790 AT5G37780.2 35.537 121 76 1 71 189 41 161 8.41e-18 77.0
Msa0651790 AT3G50360.1 34.058 138 89 1 57 192 27 164 9.10e-18 77.0
Msa0651790 AT3G25600.1 28.571 140 96 1 57 192 12 151 1.22e-17 76.6
Msa0651790 AT3G50770.1 31.633 196 108 6 9 190 21 204 1.27e-17 77.8
Msa0651790 AT2G41110.2 34.091 132 85 1 60 189 27 158 1.59e-17 76.3
Msa0651790 AT5G04870.1 32.353 170 108 3 23 189 421 586 4.44e-17 79.0
Msa0651790 AT1G66410.4 36.364 110 68 1 82 189 1 110 8.06e-17 73.2
Msa0651790 AT1G66410.3 36.364 110 68 1 82 189 1 110 8.06e-17 73.2
Msa0651790 AT3G43810.4 36.364 110 68 1 82 189 1 110 2.09e-16 72.0
Msa0651790 AT3G43810.3 36.364 110 68 1 82 189 1 110 2.09e-16 72.0
Msa0651790 AT1G76650.2 32.836 134 79 2 57 189 43 166 4.77e-16 72.8
Msa0651790 AT1G32250.1 26.027 146 100 1 55 192 14 159 4.82e-16 72.4
Msa0651790 AT3G10660.2 32.353 170 108 3 23 189 457 622 6.13e-16 75.9
Msa0651790 AT3G10660.1 32.353 170 108 3 23 189 457 622 6.13e-16 75.9
Msa0651790 AT4G14640.2 33.884 121 78 1 71 189 9 129 6.25e-16 71.2
Msa0651790 AT4G37010.2 32.609 138 91 2 57 192 31 168 1.07e-15 71.6
Msa0651790 AT4G37010.1 32.609 138 91 2 57 192 27 164 1.13e-15 71.6
Msa0651790 AT4G04695.1 34.586 133 85 2 58 189 337 468 5.96e-15 72.8
Msa0651790 AT3G03410.1 32.824 131 81 1 59 189 4 127 6.18e-15 68.9
Msa0651790 AT4G20780.1 31.395 172 93 5 42 189 13 183 6.33e-15 70.1
Msa0651790 AT5G23580.1 33.083 133 86 3 58 189 328 458 7.26e-15 72.4
Msa0651790 AT4G04700.1 33.835 133 86 2 58 189 337 468 1.10e-14 72.0
Msa0651790 AT4G04720.2 31.852 135 86 3 58 189 385 516 1.80e-14 71.2
Msa0651790 AT4G04720.1 31.852 135 86 3 58 189 385 516 1.80e-14 71.2
Msa0651790 AT2G41100.2 32.558 129 68 1 84 193 3 131 2.62e-14 68.6
Msa0651790 AT3G03000.1 30.496 141 95 1 55 192 18 158 3.08e-14 67.8
Msa0651790 AT5G44460.1 32.530 166 93 7 41 189 11 174 3.66e-14 67.8
Msa0651790 AT4G23650.1 33.083 133 87 2 58 189 384 515 3.82e-14 70.5
Msa0651790 AT3G51920.1 31.008 129 87 1 66 192 21 149 5.10e-14 67.0
Msa0651790 AT5G12480.2 35.294 136 82 3 58 189 271 404 6.26e-14 69.7
Msa0651790 AT1G18890.1 30.488 164 113 1 27 189 338 501 7.52e-14 69.7
Msa0651790 AT5G12480.1 35.294 136 82 3 58 189 365 498 8.79e-14 69.3
Msa0651790 AT1G21550.1 33.557 149 87 4 54 191 7 154 2.29e-13 65.1
Msa0651790 AT5G12180.1 32.353 136 84 3 58 189 379 510 7.75e-13 66.6
Msa0651790 AT5G19360.1 32.353 136 84 3 58 189 374 505 8.34e-13 66.6
Msa0651790 AT4G21940.1 31.111 135 87 3 58 189 407 538 2.13e-12 65.5
Msa0651790 AT4G21940.2 31.111 135 87 3 58 189 407 538 2.23e-12 65.5
Msa0651790 AT4G09570.1 31.852 135 85 4 58 189 331 461 2.30e-12 65.1
Msa0651790 AT1G35670.1 31.852 135 85 4 58 189 332 462 3.82e-12 64.7
Msa0651790 AT3G20410.2 29.630 135 89 3 58 189 397 528 6.26e-12 63.9
Msa0651790 AT3G20410.1 29.630 135 89 3 58 189 397 528 6.26e-12 63.9
Msa0651790 AT1G50700.1 29.630 135 89 3 58 189 379 510 6.74e-12 63.9
Msa0651790 AT4G38230.1 31.034 145 81 4 58 193 186 320 1.21e-11 62.8
Msa0651790 AT4G38230.4 31.034 145 81 4 58 193 330 464 1.25e-11 63.2
Msa0651790 AT4G38230.3 31.034 145 81 4 58 193 330 464 1.25e-11 63.2
Msa0651790 AT4G38230.2 31.034 145 81 4 58 193 360 494 1.34e-11 63.2
Msa0651790 AT4G04710.3 30.827 133 90 2 58 189 324 455 1.46e-11 62.8
Msa0651790 AT4G04710.2 30.827 133 90 2 58 189 338 469 1.46e-11 62.8
Msa0651790 AT3G51850.1 26.829 164 119 1 27 189 329 492 1.77e-11 62.8
Msa0651790 AT4G04710.1 30.827 133 90 2 58 189 351 482 1.87e-11 62.8
Msa0651790 AT2G17290.1 30.882 136 85 4 58 189 391 521 1.96e-11 62.4
Msa0651790 AT2G17290.2 30.882 136 85 4 58 189 391 521 1.96e-11 62.4
Msa0651790 AT5G19450.2 34.559 136 83 3 58 189 363 496 2.74e-11 62.0
Msa0651790 AT5G19450.1 34.559 136 83 3 58 189 363 496 2.74e-11 62.0
Msa0651790 AT4G04740.4 28.148 135 91 3 58 189 374 505 2.96e-11 62.0
Msa0651790 AT4G04740.6 28.148 135 91 3 58 189 374 505 2.96e-11 62.0
Msa0651790 AT4G04740.5 28.148 135 91 3 58 189 374 505 2.96e-11 62.0
Msa0651790 AT4G04740.3 28.148 135 91 3 58 189 374 505 2.96e-11 62.0
Msa0651790 AT4G04740.1 28.148 135 91 3 58 189 374 505 2.96e-11 62.0
Msa0651790 AT4G04740.7 28.148 135 91 3 58 189 374 505 2.96e-11 62.0
Msa0651790 AT1G74740.1 30.714 140 93 2 54 189 358 497 4.18e-11 61.6

Find 52 sgRNAs with CRISPR-Local

Find 55 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATCAATTTCGAAGAATTTA+TGG 0.163356 4_3:+59290222 Msa0651790:CDS
TCTCCATGCCTAGAGATTTC+AGG 0.242652 4_3:-59290141 None:intergenic
AGAAGAAGGGTGGCGGGATT+AGG 0.344511 4_3:+59290251 Msa0651790:CDS
TTCTCTTTGAGAGGTTGTAT+TGG 0.359751 4_3:-59289925 None:intergenic
TAAAGAAATGTTGTGGAAGC+TGG 0.364619 4_3:+59290345 Msa0651790:CDS
ATCTCTAGGCATGGAGAAAT+CGG 0.392953 4_3:+59290147 Msa0651790:CDS
AGAGGTTGTATTGGAAATCA+AGG 0.420314 4_3:-59289916 None:intergenic
AAATTTGATTCCAATAAAGA+TGG 0.423505 4_3:+59290088 Msa0651790:CDS
AGATGGGAAAATATCTCAAC+AGG 0.423802 4_3:+59290105 Msa0651790:CDS
CTCCATGCCTAGAGATTTCA+GGG 0.434046 4_3:-59290140 None:intergenic
ATTTATGGAGGCACAGAAGA+AGG 0.474383 4_3:+59290237 Msa0651790:CDS
TCAAAGGACAAACAGAACTC+TGG 0.476930 4_3:+59289977 Msa0651790:CDS
TTTCGGGTGGTTGACTTGGA+TGG 0.482059 4_3:+59290190 Msa0651790:CDS
GTGGTTGACTTGGATGGAGA+TGG 0.497520 4_3:+59290196 Msa0651790:CDS
TCAGGTACTTTCTCTTTGAG+AGG 0.501193 4_3:-59289934 None:intergenic
CAAAGGACAAACAGAACTCT+GGG 0.507659 4_3:+59289978 Msa0651790:CDS
ACATTTGATAAGAATTGTGA+TGG 0.509502 4_3:+59290301 Msa0651790:CDS
GGTTGACATGAACGAGTTTG+TGG 0.509979 4_3:+59290435 Msa0651790:CDS
AGAGTCGTGACGGCTTCCTC+AGG 0.512598 4_3:-59289952 None:intergenic
GGTGCAGCCTTGAGGACTGC+AGG 0.514632 4_3:+59290374 Msa0651790:CDS
TCAAAGAACAGACAAAACTC+TGG 0.529354 4_3:+59290013 Msa0651790:CDS
GAGGCACAGAAGAAGGGTGG+CGG 0.540486 4_3:+59290244 Msa0651790:CDS
CAGCATTCAAACATGTCTCA+TGG 0.541379 4_3:-59290473 None:intergenic
AAAGCTACCCTGAAATCTCT+AGG 0.549647 4_3:+59290133 Msa0651790:CDS
AGGCACAGAAGAAGGGTGGC+GGG 0.550603 4_3:+59290245 Msa0651790:CDS
GAAGCTCTCATAAAATGTCA+AGG 0.557226 4_3:+59289885 None:intergenic
TCTCATGGATTGAGTCATCA+TGG 0.558622 4_3:-59290458 None:intergenic
AATTTGATTCCAATAAAGAT+GGG 0.558831 4_3:+59290089 Msa0651790:CDS
TCCTTTGAGAAGAGTCGTGA+CGG 0.561303 4_3:-59289962 None:intergenic
CCATTCGCCTGCAGTCCTCA+AGG 0.565957 4_3:-59290381 None:intergenic
GGGTGGCGGGATTAGGTCGT+TGG 0.568531 4_3:+59290258 Msa0651790:CDS
CCTTGAGGACTGCAGGCGAA+TGG 0.569437 4_3:+59290381 Msa0651790:CDS
AAGAAATTAAAGAAATGTTG+TGG 0.574845 4_3:+59290338 Msa0651790:CDS
CAAAGAACAGACAAAACTCT+GGG 0.588758 4_3:+59290014 Msa0651790:CDS
TACCCTGAAATCTCTAGGCA+TGG 0.589415 4_3:+59290138 Msa0651790:CDS
TGTTGTGGAAGCTGGAAGAA+AGG 0.589500 4_3:+59290353 Msa0651790:CDS
ACACCATCTTCATCTCATCA+AGG 0.589556 4_3:-59290060 None:intergenic
ATCTTCATCTCATCAAGGCT+AGG 0.593507 4_3:-59290055 None:intergenic
GGAAGAAAGGTGCAGCCTTG+AGG 0.598195 4_3:+59290366 Msa0651790:CDS
CAGGCGAATGGTGAGAGCAG+TGG 0.606356 4_3:+59290393 Msa0651790:CDS
TTTATGGAGGCACAGAAGAA+GGG 0.608726 4_3:+59290238 Msa0651790:CDS
GTGAGAGCAGTGGACACTGA+TGG 0.611578 4_3:+59290403 Msa0651790:CDS
AAGCTCTCATAAAATGTCAA+GGG 0.613663 4_3:+59289886 None:intergenic
CATTTGATAAGAATTGTGAT+GGG 0.619852 4_3:+59290302 Msa0651790:CDS
GCAGTGGACACTGATGGAGA+TGG 0.626893 4_3:+59290409 Msa0651790:CDS
GCCGTCACGACTCTTCTCAA+AGG 0.629350 4_3:+59289961 Msa0651790:CDS
CAATTTCGAAGAATTTATGG+AGG 0.644323 4_3:+59290225 Msa0651790:CDS
TAGCCTTGATGAGATGAAGA+TGG 0.657485 4_3:+59290057 Msa0651790:CDS
GGACACTGATGGAGATGGCA+TGG 0.659167 4_3:+59290414 Msa0651790:CDS
TCTCAAAGAGAAAGTACCTG+AGG 0.659784 4_3:+59289936 Msa0651790:CDS
ATGGAGGCACAGAAGAAGGG+TGG 0.680107 4_3:+59290241 Msa0651790:CDS
AAAGGACAAACAGAACTCTG+GGG 0.762759 4_3:+59289979 Msa0651790:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTTGATTCCAATAAAGA+TGG + chr4_3:59290088-59290107 Msa0651790:CDS 20.0%
!! AATTTGATTCCAATAAAGAT+GGG + chr4_3:59290089-59290108 Msa0651790:CDS 20.0%
!! AAGAAATTAAAGAAATGTTG+TGG + chr4_3:59290338-59290357 Msa0651790:CDS 20.0%
!! AGATATTTTCCCATCTTTAT+TGG - chr4_3:59290101-59290120 None:intergenic 25.0%
! CATCAATTTCGAAGAATTTA+TGG + chr4_3:59290222-59290241 Msa0651790:CDS 25.0%
! ACATTTGATAAGAATTGTGA+TGG + chr4_3:59290301-59290320 Msa0651790:CDS 25.0%
! CATTTGATAAGAATTGTGAT+GGG + chr4_3:59290302-59290321 Msa0651790:CDS 25.0%
!! ATGAAGTGCCACATATTTTT+CGG + chr4_3:59290173-59290192 Msa0651790:CDS 30.0%
CAATTTCGAAGAATTTATGG+AGG + chr4_3:59290225-59290244 Msa0651790:CDS 30.0%
AGAGGTTGTATTGGAAATCA+AGG - chr4_3:59289919-59289938 None:intergenic 35.0%
! TTCTCTTTGAGAGGTTGTAT+TGG - chr4_3:59289928-59289947 None:intergenic 35.0%
TCAAAGAACAGACAAAACTC+TGG + chr4_3:59290013-59290032 Msa0651790:CDS 35.0%
CAAAGAACAGACAAAACTCT+GGG + chr4_3:59290014-59290033 Msa0651790:CDS 35.0%
AGATGGGAAAATATCTCAAC+AGG + chr4_3:59290105-59290124 Msa0651790:CDS 35.0%
!! TGAAGTGCCACATATTTTTC+GGG + chr4_3:59290174-59290193 Msa0651790:CDS 35.0%
TAAAGAAATGTTGTGGAAGC+TGG + chr4_3:59290345-59290364 Msa0651790:CDS 35.0%
TCAGGTACTTTCTCTTTGAG+AGG - chr4_3:59289937-59289956 None:intergenic 40.0%
!! TCTCAAAGAGAAAGTACCTG+AGG + chr4_3:59289936-59289955 Msa0651790:CDS 40.0%
TCAAAGGACAAACAGAACTC+TGG + chr4_3:59289977-59289996 Msa0651790:CDS 40.0%
CAAAGGACAAACAGAACTCT+GGG + chr4_3:59289978-59289997 Msa0651790:CDS 40.0%
AAAGGACAAACAGAACTCTG+GGG + chr4_3:59289979-59289998 Msa0651790:CDS 40.0%
ATCTTCATCTCATCAAGGCT+AGG - chr4_3:59290058-59290077 None:intergenic 40.0%
! TAGCCTTGATGAGATGAAGA+TGG + chr4_3:59290057-59290076 Msa0651790:CDS 40.0%
ACACCATCTTCATCTCATCA+AGG - chr4_3:59290063-59290082 None:intergenic 40.0%
AAAGCTACCCTGAAATCTCT+AGG + chr4_3:59290133-59290152 Msa0651790:CDS 40.0%
ATCTCTAGGCATGGAGAAAT+CGG + chr4_3:59290147-59290166 Msa0651790:CDS 40.0%
TCAACCACCCGAAAAATATG+TGG - chr4_3:59290184-59290203 None:intergenic 40.0%
!!! TATTTTTCGGGTGGTTGACT+TGG + chr4_3:59290186-59290205 Msa0651790:CDS 40.0%
! ATTTATGGAGGCACAGAAGA+AGG + chr4_3:59290237-59290256 Msa0651790:CDS 40.0%
! TTTATGGAGGCACAGAAGAA+GGG + chr4_3:59290238-59290257 Msa0651790:CDS 40.0%
! TCTCATGGATTGAGTCATCA+TGG - chr4_3:59290461-59290480 None:intergenic 40.0%
! TCCTTTGAGAAGAGTCGTGA+CGG - chr4_3:59289965-59289984 None:intergenic 45.0%
TACCCTGAAATCTCTAGGCA+TGG + chr4_3:59290138-59290157 Msa0651790:CDS 45.0%
CTCCATGCCTAGAGATTTCA+GGG - chr4_3:59290143-59290162 None:intergenic 45.0%
TCTCCATGCCTAGAGATTTC+AGG - chr4_3:59290144-59290163 None:intergenic 45.0%
!!! AGTGCCACATATTTTTCGGG+TGG + chr4_3:59290177-59290196 Msa0651790:CDS 45.0%
TGTTGTGGAAGCTGGAAGAA+AGG + chr4_3:59290353-59290372 Msa0651790:CDS 45.0%
GGTTGACATGAACGAGTTTG+TGG + chr4_3:59290435-59290454 Msa0651790:CDS 45.0%
!! TTTCGGGTGGTTGACTTGGA+TGG + chr4_3:59290190-59290209 Msa0651790:CDS 50.0%
!! GTGGTTGACTTGGATGGAGA+TGG + chr4_3:59290196-59290215 Msa0651790:CDS 50.0%
GCCGTCACGACTCTTCTCAA+AGG + chr4_3:59289961-59289980 Msa0651790:CDS 55.0%
! ATGGAGGCACAGAAGAAGGG+TGG + chr4_3:59290241-59290260 Msa0651790:CDS 55.0%
!! AGAAGAAGGGTGGCGGGATT+AGG + chr4_3:59290251-59290270 Msa0651790:CDS 55.0%
! GGAAGAAAGGTGCAGCCTTG+AGG + chr4_3:59290366-59290385 Msa0651790:CDS 55.0%
GTGAGAGCAGTGGACACTGA+TGG + chr4_3:59290403-59290422 Msa0651790:CDS 55.0%
GCAGTGGACACTGATGGAGA+TGG + chr4_3:59290409-59290428 Msa0651790:CDS 55.0%
GGACACTGATGGAGATGGCA+TGG + chr4_3:59290414-59290433 Msa0651790:CDS 55.0%
AGAGTCGTGACGGCTTCCTC+AGG - chr4_3:59289955-59289974 None:intergenic 60.0%
GAGGCACAGAAGAAGGGTGG+CGG + chr4_3:59290244-59290263 Msa0651790:CDS 60.0%
AGGCACAGAAGAAGGGTGGC+GGG + chr4_3:59290245-59290264 Msa0651790:CDS 60.0%
CCATTCGCCTGCAGTCCTCA+AGG - chr4_3:59290384-59290403 None:intergenic 60.0%
CCTTGAGGACTGCAGGCGAA+TGG + chr4_3:59290381-59290400 Msa0651790:CDS 60.0%
CAGGCGAATGGTGAGAGCAG+TGG + chr4_3:59290393-59290412 Msa0651790:CDS 60.0%
!! GGGTGGCGGGATTAGGTCGT+TGG + chr4_3:59290258-59290277 Msa0651790:CDS 65.0%
GGTGCAGCCTTGAGGACTGC+AGG + chr4_3:59290374-59290393 Msa0651790:CDS 65.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 59289899 59290489 59289899 ID=Msa0651790;Name=Msa0651790
chr4_3 mRNA 59289899 59290489 59289899 ID=Msa0651790-mRNA-1;Parent=Msa0651790;Name=Msa0651790-mRNA-1;_AED=0.34;_eAED=0.34;_QI=0|-1|0|1|-1|1|1|0|196
chr4_3 exon 59289899 59290489 59289899 ID=Msa0651790-mRNA-1:exon:15865;Parent=Msa0651790-mRNA-1
chr4_3 CDS 59289899 59290489 59289899 ID=Msa0651790-mRNA-1:cds;Parent=Msa0651790-mRNA-1
Gene Sequence

>Msa0651790

ATGTCAAGGGCAAGTTTCCTTGATTTCCAATACAACCTCTCAAAGAGAAAGTACCTGAGGAAGCCGTCACGACTCTTCTCAAAGGACAAACAGAACTCTGGGGTAACTATGTTCTCAAAGAACAGACAAAACTCTGGGCTAAAATATATTTTTCAGCCTAGCCTTGATGAGATGAAGATGGTGTTTGACAAATTTGATTCCAATAAAGATGGGAAAATATCTCAACAGGAGTACAAAGCTACCCTGAAATCTCTAGGCATGGAGAAATCGGTGAATGAAGTGCCACATATTTTTCGGGTGGTTGACTTGGATGGAGATGGATTCATCAATTTCGAAGAATTTATGGAGGCACAGAAGAAGGGTGGCGGGATTAGGTCGTTGGATATACAGACTGCGTTTCGAACATTTGATAAGAATTGTGATGGGAAAATAAGTGCTGAAGAAATTAAAGAAATGTTGTGGAAGCTGGAAGAAAGGTGCAGCCTTGAGGACTGCAGGCGAATGGTGAGAGCAGTGGACACTGATGGAGATGGCATGGTTGACATGAACGAGTTTGTGGCCATGATGACTCAATCCATGAGACATGTTTGA
Protein sequence

>Msa0651790

MSRASFLDFQYNLSKRKYLRKPSRLFSKDKQNSGVTMFSKNRQNSGLKYIFQPSLDEMKMVFDKFDSNKDGKISQQEYKATLKSLGMEKSVNEVPHIFRVVDLDGDGFINFEEFMEAQKKGGGIRSLDIQTAFRTFDKNCDGKISAEEIKEMLWKLEERCSLEDCRRMVRAVDTDGDGMVDMNEFVAMMTQSMRHV*