AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0148340


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0148340 MtrunA17_Chr1g0150721 94.406 143 7 1 1 143 1 142 2.65e-97 276
Msa0148340 MtrunA17_Chr3g0136431 59.060 149 49 4 1 143 1 143 8.34e-55 169
Msa0148340 MtrunA17_Chr4g0008841 31.034 145 71 4 2 138 13 136 9.08e-18 74.3
Msa0148340 MtrunA17_Chr7g0240831 34.646 127 62 3 2 128 22 127 2.45e-15 68.2
Msa0148340 MtrunA17_Chr7g0240841 34.646 127 62 3 2 128 22 127 2.45e-15 68.2
Msa0148340 MtrunA17_Chr7g0240861 30.000 140 76 4 2 139 27 146 5.56e-15 67.4
Msa0148340 MtrunA17_Chr4g0032291 32.331 133 65 4 5 133 46 157 1.20e-14 67.0
Msa0148340 MtrunA17_Chr4g0032581 32.258 124 62 4 5 128 35 136 1.26e-13 63.9
Msa0148340 MtrunA17_Chr4g0032571 27.407 135 76 2 1 135 27 139 5.47e-13 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0148340 AT5G20650.1 55.102 147 61 3 1 143 1 146 3.66e-50 157
Msa0148340 AT2G26975.1 32.847 137 65 3 1 131 24 139 2.30e-19 79.0
Msa0148340 AT5G59040.1 34.375 128 63 2 1 128 29 135 2.81e-17 73.9
Msa0148340 AT2G37925.1 31.250 128 70 2 1 128 29 138 2.00e-14 66.2
Msa0148340 AT5G59030.1 27.778 144 80 3 5 142 45 170 4.37e-13 63.2
Msa0148340 AT3G46900.1 26.761 142 78 4 7 142 35 156 9.32e-13 62.4

Find 30 sgRNAs with CRISPR-Local

Find 40 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCAACATTCTACCAATATT+TGG 0.138215 1_4:-8597741 Msa0148340:CDS
GAGATTCGGACTCCTCTTCT+TGG 0.249119 1_4:-8597656 Msa0148340:CDS
ACCAATATTTGGAAAATATT+CGG 0.254322 1_4:-8597730 Msa0148340:CDS
TCCTCTTCTTGGGTTGAATA+AGG 0.262656 1_4:-8597645 Msa0148340:CDS
TGACACTCTTATTTGATTCA+TGG 0.286816 1_4:-8597817 Msa0148340:CDS
TGATGCACATGACATTATAT+TGG 0.315744 1_4:-8597850 Msa0148340:CDS
GATGCACATGACATTATATT+GGG 0.333053 1_4:-8597849 Msa0148340:CDS
CGTTTGGTTACTTCTTGTTT+AGG 0.336421 1_4:-8597499 Msa0148340:CDS
ATTCATGGAAAACCAATTCA+TGG 0.342364 1_4:-8597802 Msa0148340:CDS
TTGGCTATAATGTCGTTTAA+TGG 0.343696 1_4:-8597554 Msa0148340:CDS
TTTGGTGTGAATTCTGCTAT+TGG 0.364233 1_4:-8597590 Msa0148340:CDS
ATTGTGGTTGGTTTAACGTT+TGG 0.388729 1_4:-8597515 Msa0148340:CDS
TAGATAACTCGTCCATGAAT+TGG 0.393378 1_4:+8597790 None:intergenic
AGATTCGGACTCCTCTTCTT+GGG 0.397569 1_4:-8597655 Msa0148340:CDS
TTGGTGTGAATTCTGCTATT+GGG 0.404245 1_4:-8597589 Msa0148340:CDS
ATTTGGAAAATATTCGGATT+CGG 0.414816 1_4:-8597724 Msa0148340:CDS
AGTCGCCGGTGCCGGAGATT+CGG 0.431491 1_4:-8597670 Msa0148340:CDS
TATTGGGTATTTGTTGATGT+TGG 0.437573 1_4:-8597573 Msa0148340:CDS
TTGGGTTGAATAAGGTTTCG+AGG 0.486516 1_4:-8597637 Msa0148340:CDS
AGAAGAGGAGTCCGAATCTC+CGG 0.488903 1_4:+8597659 None:intergenic
GAGGACGCAGTCGCCGGTGC+CGG 0.509370 1_4:-8597678 Msa0148340:CDS
TGAGGGGAGGACGCAGTCGC+CGG 0.519658 1_4:-8597684 Msa0148340:CDS
ACCTTATTCAACCCAAGAAG+AGG 0.545808 1_4:+8597644 None:intergenic
GGAGTCCGAATCTCCGGCAC+CGG 0.550760 1_4:+8597665 None:intergenic
TCAGAGTGAAGAAGAGGCTG+CGG 0.552122 1_4:-8597474 Msa0148340:CDS
TAGGAGTCAGAGTGAAGAAG+AGG 0.579273 1_4:-8597480 Msa0148340:CDS
CACAAACTAAGCACATGCAC+AGG 0.579857 1_4:+8597436 None:intergenic
GAATAAGGTTTCGAGGAACA+AGG 0.583225 1_4:-8597630 Msa0148340:CDS
GCTATAATGTCGTTTAATGG+TGG 0.610087 1_4:-8597551 Msa0148340:CDS
ATGCACATGACATTATATTG+GGG 0.668922 1_4:-8597848 Msa0148340:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACCAATATTTGGAAAATATT+CGG - chr1_4:8597563-8597582 Msa0148340:CDS 20.0%
!! TCCGAATATTTTCCAAATAT+TGG + chr1_4:8597567-8597586 None:intergenic 25.0%
! ATTTGGAAAATATTCGGATT+CGG - chr1_4:8597569-8597588 Msa0148340:CDS 25.0%
!!! GTTTTTGAATCGGTTTTATT+TGG - chr1_4:8597685-8597704 Msa0148340:CDS 25.0%
TGATGCACATGACATTATAT+TGG - chr1_4:8597443-8597462 Msa0148340:CDS 30.0%
GATGCACATGACATTATATT+GGG - chr1_4:8597444-8597463 Msa0148340:CDS 30.0%
ATGCACATGACATTATATTG+GGG - chr1_4:8597445-8597464 Msa0148340:CDS 30.0%
! TGACACTCTTATTTGATTCA+TGG - chr1_4:8597476-8597495 Msa0148340:CDS 30.0%
ATTCATGGAAAACCAATTCA+TGG - chr1_4:8597491-8597510 Msa0148340:CDS 30.0%
TGCAACATTCTACCAATATT+TGG - chr1_4:8597552-8597571 Msa0148340:CDS 30.0%
! TATTGGGTATTTGTTGATGT+TGG - chr1_4:8597720-8597739 Msa0148340:CDS 30.0%
! TTGGCTATAATGTCGTTTAA+TGG - chr1_4:8597739-8597758 Msa0148340:CDS 30.0%
TAGATAACTCGTCCATGAAT+TGG + chr1_4:8597506-8597525 None:intergenic 35.0%
! ATTCGGTTGAAGTTTTTCTC+TGG - chr1_4:8597586-8597605 Msa0148340:CDS 35.0%
! TTGAAGTTTTTCTCTGGTGA+GGG - chr1_4:8597592-8597611 Msa0148340:CDS 35.0%
! GAGGAACAAGGTTTTTGAAT+CGG - chr1_4:8597675-8597694 Msa0148340:CDS 35.0%
! TTTGGTGTGAATTCTGCTAT+TGG - chr1_4:8597703-8597722 Msa0148340:CDS 35.0%
TTGGTGTGAATTCTGCTATT+GGG - chr1_4:8597704-8597723 Msa0148340:CDS 35.0%
! GCTATAATGTCGTTTAATGG+TGG - chr1_4:8597742-8597761 Msa0148340:CDS 35.0%
!! GTTTAATGGTGGTGTTTTTG+TGG - chr1_4:8597753-8597772 Msa0148340:CDS 35.0%
!! ATTGTGGTTGGTTTAACGTT+TGG - chr1_4:8597778-8597797 Msa0148340:CDS 35.0%
! CGTTTGGTTACTTCTTGTTT+AGG - chr1_4:8597794-8597813 Msa0148340:CDS 35.0%
! GTTGAAGTTTTTCTCTGGTG+AGG - chr1_4:8597591-8597610 Msa0148340:CDS 40.0%
! TGAAGTTTTTCTCTGGTGAG+GGG - chr1_4:8597593-8597612 Msa0148340:CDS 40.0%
!! TCCTCTTCTTGGGTTGAATA+AGG - chr1_4:8597648-8597667 Msa0148340:CDS 40.0%
ACCTTATTCAACCCAAGAAG+AGG + chr1_4:8597652-8597671 None:intergenic 40.0%
!! TTGGGTTGAATAAGGTTTCG+AGG - chr1_4:8597656-8597675 Msa0148340:CDS 40.0%
GAATAAGGTTTCGAGGAACA+AGG - chr1_4:8597663-8597682 Msa0148340:CDS 40.0%
!! TGGTGTTTTTGTGGCTATTG+TGG - chr1_4:8597762-8597781 Msa0148340:CDS 40.0%
! GTTTTTGTGGCTATTGTGGT+TGG - chr1_4:8597766-8597785 Msa0148340:CDS 40.0%
AGATTCGGACTCCTCTTCTT+GGG - chr1_4:8597638-8597657 Msa0148340:CDS 45.0%
TAGGAGTCAGAGTGAAGAAG+AGG - chr1_4:8597813-8597832 Msa0148340:CDS 45.0%
! AGTTTTTCTCTGGTGAGGGG+AGG - chr1_4:8597596-8597615 Msa0148340:CDS 50.0%
AGAAGAGGAGTCCGAATCTC+CGG + chr1_4:8597637-8597656 None:intergenic 50.0%
GAGATTCGGACTCCTCTTCT+TGG - chr1_4:8597637-8597656 Msa0148340:CDS 50.0%
TCAGAGTGAAGAAGAGGCTG+CGG - chr1_4:8597819-8597838 Msa0148340:CDS 50.0%
! AGTCGCCGGTGCCGGAGATT+CGG - chr1_4:8597623-8597642 Msa0148340:CDS 65.0%
GGAGTCCGAATCTCCGGCAC+CGG + chr1_4:8597631-8597650 None:intergenic 65.0%
TGAGGGGAGGACGCAGTCGC+CGG - chr1_4:8597609-8597628 Msa0148340:CDS 70.0%
GAGGACGCAGTCGCCGGTGC+CGG - chr1_4:8597615-8597634 Msa0148340:CDS 75.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 8597442 8597873 8597442 ID=Msa0148340;Name=Msa0148340
chr1_4 mRNA 8597442 8597873 8597442 ID=Msa0148340-mRNA-1;Parent=Msa0148340;Name=Msa0148340-mRNA-1;_AED=0.02;_eAED=0.02;_QI=0|-1|0|1|-1|1|1|0|143
chr1_4 exon 8597442 8597873 8597442 ID=Msa0148340-mRNA-1:exon:3696;Parent=Msa0148340-mRNA-1
chr1_4 CDS 8597442 8597873 8597442 ID=Msa0148340-mRNA-1:cds;Parent=Msa0148340-mRNA-1
Gene Sequence

>Msa0148340

ATGATGCACATGACATTATATTGGGGAAAAAACGTGACACTCTTATTTGATTCATGGAAAACCAATTCATGGACGAGTTATCTACTGAGTTTAATTGCATGTTTCATGATTGCAACATTCTACCAATATTTGGAAAATATTCGGATTCGGTTGAAGTTTTTCTCTGGTGAGGGGAGGACGCAGTCGCCGGTGCCGGAGATTCGGACTCCTCTTCTTGGGTTGAATAAGGTTTCGAGGAACAAGGTTTTTGAATCGGTTTTATTTGGTGTGAATTCTGCTATTGGGTATTTGTTGATGTTGGCTATAATGTCGTTTAATGGTGGTGTTTTTGTGGCTATTGTGGTTGGTTTAACGTTTGGTTACTTCTTGTTTAGGAGTCAGAGTGAAGAAGAGGCTGCGGTTGTTGTTGATAATTCCTGTGCATGTGCTTAG

Protein sequence

>Msa0148340

MMHMTLYWGKNVTLLFDSWKTNSWTSYLLSLIACFMIATFYQYLENIRIRLKFFSGEGRTQSPVPEIRTPLLGLNKVSRNKVFESVLFGVNSAIGYLLMLAIMSFNGGVFVAIVVGLTFGYFLFRSQSEEEAAVVVDNSCACA*