AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0611550

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0611550 MtrunA17_Chr4g0044961 99.490 196 1 0 1 196 1 196 7.15e-145 400
Msa0611550 MtrunA17_Chr5g0402331 46.632 193 88 6 1 189 1 182 6.38e-48 155
Msa0611550 MtrunA17_Chr5g0402361 51.974 152 67 3 42 189 205 354 2.84e-45 153
Msa0611550 MtrunA17_Chr5g0402361 45.789 190 93 3 1 189 1 181 5.00e-45 152
Msa0611550 MtrunA17_Chr5g0402361 30.675 163 83 2 56 188 119 281 1.80e-13 67.8
Msa0611550 MtrunA17_Chr8g0391691 43.972 141 77 1 57 195 36 176 1.57e-32 115
Msa0611550 MtrunA17_Chr1g0161211 42.446 139 78 1 56 192 45 183 1.23e-31 113
Msa0611550 MtrunA17_Chr1g0161211 39.394 66 40 0 128 193 46 111 1.76e-11 60.5
Msa0611550 MtrunA17_Chr3g0111371 40.876 137 79 1 56 192 4 138 5.63e-31 110
Msa0611550 MtrunA17_Chr5g0434951 43.066 137 77 1 56 191 3 139 1.23e-30 109
Msa0611550 MtrunA17_Chr8g0392951 38.889 144 87 1 50 192 17 160 5.18e-29 105
Msa0611550 MtrunA17_Chr1g0169581 39.286 140 81 1 56 191 3 142 3.16e-27 100
Msa0611550 MtrunA17_Chr6g0482731 38.889 144 82 3 55 194 24 165 2.06e-25 97.1
Msa0611550 MtrunA17_Chr8g0351681 37.838 148 82 4 57 194 49 196 4.82e-25 96.7
Msa0611550 MtrunA17_Chr7g0256171 37.956 137 81 2 57 189 5 141 3.28e-24 93.2
Msa0611550 MtrunA17_Chr4g0033601 37.681 138 82 2 56 189 4 141 2.05e-23 90.9
Msa0611550 MtrunA17_Chr7g0245911 38.621 145 78 4 56 189 46 190 3.13e-23 91.7
Msa0611550 MtrunA17_Chr4g0057661 35.821 134 85 1 57 189 6 139 3.52e-22 87.4
Msa0611550 MtrunA17_Chr7g0254731 35.766 137 86 1 55 189 10 146 5.09e-22 87.4
Msa0611550 MtrunA17_Chr2g0329371 36.429 140 83 2 56 189 63 202 6.60e-22 88.6
Msa0611550 MtrunA17_Chr3g0116151 34.615 130 84 1 61 189 10 139 7.52e-22 86.7
Msa0611550 MtrunA17_Chr3g0116141 34.615 130 84 1 61 189 9 138 8.78e-22 86.7
Msa0611550 MtrunA17_Chr1g0169621 33.537 164 80 2 56 190 3 166 1.29e-21 87.0
Msa0611550 MtrunA17_Chr3g0093271 36.296 135 84 1 57 189 63 197 1.85e-21 87.4
Msa0611550 MtrunA17_Chr1g0188171 36.842 133 82 1 59 189 14 146 2.78e-21 85.5
Msa0611550 MtrunA17_Chr5g0440931 35.766 137 86 1 55 189 10 146 2.96e-21 85.5
Msa0611550 MtrunA17_Chr6g0460211 35.766 137 86 1 55 189 10 146 3.19e-21 85.5
Msa0611550 MtrunA17_Chr7g0225921 36.090 133 79 3 61 189 10 140 5.80e-21 84.3
Msa0611550 MtrunA17_Chr4g0057641 35.075 134 86 1 57 189 6 139 1.26e-20 83.6
Msa0611550 MtrunA17_Chr6g0459231 34.615 130 84 1 61 189 12 141 2.14e-20 82.8
Msa0611550 MtrunA17_Chr4g0057651 35.075 134 86 1 57 189 6 139 2.57e-20 82.8
Msa0611550 MtrunA17_Chr1g0166281 36.296 135 84 1 57 189 12 146 3.06e-20 82.8
Msa0611550 MtrunA17_Chr8g0366401 30.070 143 95 1 55 192 15 157 3.49e-20 83.2
Msa0611550 MtrunA17_Chr4g0062221 30.282 142 95 1 55 192 9 150 8.42e-20 82.0
Msa0611550 MtrunA17_Chr7g0256701 35.000 140 84 3 57 189 73 212 8.60e-19 80.5
Msa0611550 MtrunA17_Chr1g0185001 34.921 126 81 1 65 189 20 145 1.06e-18 78.6
Msa0611550 MtrunA17_Chr4g0064231 35.714 154 79 3 56 189 63 216 1.83e-18 80.1
Msa0611550 MtrunA17_Chr7g0245231 31.646 158 105 2 41 195 14 171 1.42e-17 76.3
Msa0611550 MtrunA17_Chr8g0368681 27.941 136 94 1 61 192 16 151 1.65e-17 75.9
Msa0611550 MtrunA17_Chr5g0396261 35.965 114 69 1 82 191 2 115 1.00e-16 72.8
Msa0611550 MtrunA17_Chr8g0373691 34.074 135 87 2 58 190 54 188 1.01e-16 74.3
Msa0611550 MtrunA17_Chr5g0409441 32.031 128 83 2 61 185 26 152 2.34e-16 72.8
Msa0611550 MtrunA17_Chr7g0245371 32.192 146 85 2 58 189 25 170 2.57e-16 73.2
Msa0611550 MtrunA17_Chr7g0274101 34.615 130 83 1 62 189 17 146 3.75e-16 72.0
Msa0611550 MtrunA17_Chr5g0441691 33.083 133 87 2 58 189 374 505 1.49e-15 74.3
Msa0611550 MtrunA17_Chr6g0450921 30.556 180 108 4 19 195 5 170 1.64e-15 70.9
Msa0611550 MtrunA17_Chr5g0402351 47.561 82 42 1 51 131 17 98 1.66e-15 69.3
Msa0611550 MtrunA17_Chr1g0160151 29.932 147 97 2 56 196 21 167 1.82e-15 70.9
Msa0611550 MtrunA17_Chr4g0074811 28.409 176 120 3 23 195 362 534 3.42e-15 73.2
Msa0611550 MtrunA17_Chr1g0176751 33.582 134 84 3 58 189 430 560 1.63e-14 71.2
Msa0611550 MtrunA17_Chr7g0267751 28.824 170 114 3 23 189 406 571 2.22e-14 70.9
Msa0611550 MtrunA17_Chr1g0170281 29.412 170 113 3 23 189 389 554 3.05e-14 70.5
Msa0611550 MtrunA17_Chr5g0397101 33.088 136 83 3 58 189 372 503 7.34e-14 69.3
Msa0611550 MtrunA17_Chr4g0059951 32.143 140 91 2 54 189 372 511 8.02e-14 69.3
Msa0611550 MtrunA17_Chr5g0444171 33.083 133 87 2 58 189 398 529 9.63e-14 68.9
Msa0611550 MtrunA17_Chr1g0157131 29.139 151 97 4 46 189 364 511 9.82e-14 68.9
Msa0611550 MtrunA17_Chr4g0074791 29.944 177 101 6 25 195 369 528 1.29e-13 68.6
Msa0611550 MtrunA17_Chr3g0121191 29.787 141 87 2 57 195 93 223 1.86e-13 66.6
Msa0611550 MtrunA17_Chr0c02g0489761 32.331 133 88 2 58 189 384 515 2.28e-13 67.8
Msa0611550 MtrunA17_Chr8g0387671 33.083 133 87 2 58 189 372 503 2.32e-13 67.8
Msa0611550 MtrunA17_Chr1g0200601 32.593 135 84 4 58 189 336 466 5.89e-13 66.6
Msa0611550 MtrunA17_Chr3g0131181 28.736 174 115 4 21 189 296 465 6.39e-13 66.6
Msa0611550 MtrunA17_Chr8g0385341 28.235 170 115 3 23 189 301 466 4.23e-12 63.9
Msa0611550 MtrunA17_Chr3g0098951 30.216 139 90 3 58 189 389 527 4.62e-12 63.9
Msa0611550 MtrunA17_Chr5g0448721 31.206 141 78 4 58 189 402 532 8.10e-12 63.2
Msa0611550 MtrunA17_Chr4g0042491 41.429 70 41 0 123 192 109 178 1.71e-11 60.5
Msa0611550 MtrunA17_Chr7g0242561 29.412 170 107 5 27 189 331 494 2.08e-11 62.0
Msa0611550 MtrunA17_Chr3g0125311 29.932 147 89 4 57 189 13 159 4.85e-11 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0611550 AT2G15680.1 59.896 192 70 1 1 192 1 185 1.79e-81 240
Msa0611550 AT1G24620.1 45.000 140 75 1 57 194 37 176 2.93e-34 120
Msa0611550 AT1G73630.1 45.324 139 73 2 53 190 15 151 1.85e-32 115
Msa0611550 AT1G18210.2 42.222 135 76 1 56 190 22 154 9.10e-30 108
Msa0611550 AT1G18210.1 42.222 135 76 1 56 190 22 154 9.10e-30 108
Msa0611550 AT1G66400.1 40.141 142 77 2 54 189 12 151 3.55e-29 106
Msa0611550 AT5G37770.1 39.583 144 81 1 54 191 14 157 4.70e-29 106
Msa0611550 AT5G37770.2 39.583 144 81 1 54 191 14 157 4.70e-29 106
Msa0611550 AT5G17470.1 39.552 134 77 1 61 190 6 139 2.26e-25 96.3
Msa0611550 AT5G17470.1 49.123 57 29 0 58 114 78 134 3.80e-11 58.9
Msa0611550 AT5G42380.1 36.691 139 85 1 54 189 46 184 3.32e-25 97.1
Msa0611550 AT3G07490.1 38.462 143 72 3 57 189 5 141 2.27e-24 94.0
Msa0611550 AT1G05990.1 39.130 138 79 2 57 189 5 142 3.43e-24 93.6
Msa0611550 AT4G12860.1 40.146 137 78 2 57 189 5 141 5.21e-24 93.2
Msa0611550 AT4G03290.1 38.571 140 79 2 57 189 5 144 3.00e-23 91.3
Msa0611550 AT2G36180.1 38.571 140 81 2 58 192 1 140 4.05e-23 90.5
Msa0611550 AT3G03400.1 36.000 125 74 2 61 180 9 132 4.95e-23 90.1
Msa0611550 AT2G43290.1 34.247 146 85 3 53 189 63 206 4.04e-22 89.7
Msa0611550 AT1G66410.1 35.766 137 86 1 55 189 10 146 1.48e-21 86.7
Msa0611550 AT5G37780.1 35.766 137 86 1 55 189 10 146 1.48e-21 86.7
Msa0611550 AT5G21274.1 36.496 137 85 1 55 189 10 146 1.87e-21 86.3
Msa0611550 AT1G76650.1 35.821 134 85 1 57 189 43 176 3.77e-21 86.3
Msa0611550 AT1G76650.3 35.821 134 85 1 57 189 43 176 3.77e-21 86.3
Msa0611550 AT3G43810.1 35.766 137 86 1 55 189 10 146 4.40e-21 85.5
Msa0611550 AT3G43810.2 35.766 137 86 1 55 189 10 146 6.45e-21 86.7
Msa0611550 AT2G27030.3 34.507 142 91 1 55 194 10 151 1.09e-20 85.1
Msa0611550 AT3G56800.1 35.036 137 87 1 55 189 10 146 1.63e-20 84.0
Msa0611550 AT2G41110.1 35.036 137 87 1 55 189 10 146 1.63e-20 84.0
Msa0611550 AT2G27030.1 35.036 137 87 1 55 189 10 146 1.63e-20 84.0
Msa0611550 AT2G41100.7 32.911 158 87 1 55 193 64 221 2.03e-20 86.7
Msa0611550 AT2G41100.7 30.303 132 74 1 82 195 2 133 5.89e-14 68.9
Msa0611550 AT2G41100.6 32.911 158 87 1 55 193 64 221 2.03e-20 86.7
Msa0611550 AT2G41100.6 30.303 132 74 1 82 195 2 133 5.89e-14 68.9
Msa0611550 AT2G41100.3 32.911 158 87 1 55 193 64 221 2.03e-20 86.7
Msa0611550 AT2G41100.3 30.303 132 74 1 82 195 2 133 5.89e-14 68.9
Msa0611550 AT1G18530.1 30.000 140 94 1 57 192 7 146 2.73e-20 83.6
Msa0611550 AT2G41100.4 32.911 158 87 1 55 193 99 256 3.22e-20 86.7
Msa0611550 AT2G41100.4 30.818 159 92 1 55 195 10 168 5.72e-19 83.2
Msa0611550 AT2G41100.1 32.911 158 87 1 55 193 99 256 3.22e-20 86.7
Msa0611550 AT2G41100.1 30.818 159 92 1 55 195 10 168 5.72e-19 83.2
Msa0611550 AT1G76640.1 32.692 156 88 3 44 189 10 158 2.34e-19 81.3
Msa0611550 AT2G41410.1 34.320 169 100 5 30 193 49 211 2.93e-19 82.4
Msa0611550 AT3G59440.1 33.803 142 79 3 57 189 51 186 3.51e-19 81.6
Msa0611550 AT3G10190.1 36.029 136 84 3 57 189 70 205 3.93e-19 81.6
Msa0611550 AT4G14640.1 34.815 135 86 1 57 189 13 147 6.34e-19 79.7
Msa0611550 AT2G41090.1 34.815 135 87 1 55 189 10 143 1.06e-18 80.1
Msa0611550 AT3G50770.1 32.143 196 107 6 9 190 21 204 1.09e-18 80.5
Msa0611550 AT3G25600.1 29.286 140 95 1 57 192 12 151 1.14e-18 79.3
Msa0611550 AT5G04870.1 32.941 170 107 3 23 189 421 586 3.13e-18 82.4
Msa0611550 AT3G22930.1 33.571 140 91 1 52 189 30 169 3.29e-18 78.6
Msa0611550 AT5G37780.4 35.537 121 76 1 71 189 13 133 5.87e-18 76.6
Msa0611550 AT1G66410.2 35.537 121 76 1 71 189 36 156 6.91e-18 77.4
Msa0611550 AT5G37780.3 35.537 121 76 1 71 189 52 172 7.10e-18 77.8
Msa0611550 AT5G37780.2 35.537 121 76 1 71 189 41 161 7.89e-18 77.4
Msa0611550 AT3G50360.1 34.058 138 89 1 57 192 27 164 1.09e-17 77.0
Msa0611550 AT2G41110.2 34.091 132 85 1 60 189 27 158 1.72e-17 76.3
Msa0611550 AT1G76650.2 33.582 134 78 2 57 189 43 166 2.86e-17 75.9
Msa0611550 AT1G32250.1 26.712 146 99 1 55 192 14 159 5.38e-17 75.1
Msa0611550 AT1G66410.4 36.364 110 68 1 82 189 1 110 1.10e-16 72.8
Msa0611550 AT1G66410.3 36.364 110 68 1 82 189 1 110 1.10e-16 72.8
Msa0611550 AT3G43810.4 36.364 110 68 1 82 189 1 110 2.73e-16 71.6
Msa0611550 AT3G43810.3 36.364 110 68 1 82 189 1 110 2.73e-16 71.6
Msa0611550 AT3G03410.1 33.588 131 80 1 59 189 4 127 3.26e-16 72.0
Msa0611550 AT3G10660.2 32.353 170 108 3 23 189 457 622 3.76e-16 76.3
Msa0611550 AT3G10660.1 32.353 170 108 3 23 189 457 622 3.76e-16 76.3
Msa0611550 AT4G14640.2 33.884 121 78 1 71 189 9 129 4.90e-16 71.6
Msa0611550 AT4G20780.1 31.977 172 92 5 42 189 13 183 7.94e-16 72.4
Msa0611550 AT4G37010.2 32.609 138 91 2 57 192 31 168 9.51e-16 72.0
Msa0611550 AT4G37010.1 32.609 138 91 2 57 192 27 164 1.00e-15 71.6
Msa0611550 AT3G03000.1 31.206 141 94 1 55 192 18 158 1.84e-15 70.9
Msa0611550 AT2G41100.2 33.333 129 67 1 84 193 3 131 2.41e-15 71.6
Msa0611550 AT5G44460.1 33.133 166 92 7 41 189 11 174 3.36e-15 70.9
Msa0611550 AT3G51920.1 31.343 134 90 1 61 192 16 149 5.01e-15 69.7
Msa0611550 AT4G04695.1 34.586 133 85 2 58 189 337 468 5.53e-15 72.8
Msa0611550 AT5G23580.1 33.083 133 86 3 58 189 328 458 6.99e-15 72.4
Msa0611550 AT1G18890.1 31.098 164 112 1 27 189 338 501 7.20e-15 72.4
Msa0611550 AT4G04700.1 33.835 133 86 2 58 189 337 468 9.54e-15 72.0
Msa0611550 AT4G04720.2 31.852 135 86 3 58 189 385 516 1.38e-14 71.6
Msa0611550 AT4G04720.1 31.852 135 86 3 58 189 385 516 1.38e-14 71.6
Msa0611550 AT1G21550.1 34.228 149 86 4 54 191 7 154 1.41e-14 68.6
Msa0611550 AT4G23650.1 33.083 133 87 2 58 189 384 515 3.31e-14 70.5
Msa0611550 AT5G12480.2 35.294 136 82 3 58 189 271 404 6.45e-14 69.7
Msa0611550 AT5G12480.1 35.294 136 82 3 58 189 365 498 9.77e-14 69.3
Msa0611550 AT4G09570.1 32.593 135 84 4 58 189 331 461 1.48e-13 68.6
Msa0611550 AT1G35670.1 32.593 135 84 4 58 189 332 462 2.87e-13 67.8
Msa0611550 AT1G50700.1 30.370 135 88 3 58 189 379 510 6.50e-13 67.0
Msa0611550 AT5G12180.1 32.353 136 84 3 58 189 379 510 7.32e-13 66.6
Msa0611550 AT5G19360.1 32.353 136 84 3 58 189 374 505 7.58e-13 66.6
Msa0611550 AT4G38230.2 31.724 145 80 4 58 193 360 494 1.10e-12 66.2
Msa0611550 AT4G38230.1 31.724 145 80 4 58 193 186 320 1.16e-12 65.9
Msa0611550 AT4G38230.4 31.724 145 80 4 58 193 330 464 1.21e-12 66.2
Msa0611550 AT4G38230.3 31.724 145 80 4 58 193 330 464 1.21e-12 66.2
Msa0611550 AT4G04710.2 31.579 133 89 2 58 189 338 469 1.22e-12 65.9
Msa0611550 AT4G04710.3 31.579 133 89 2 58 189 324 455 1.34e-12 65.9
Msa0611550 AT4G21940.2 31.111 135 87 3 58 189 407 538 1.34e-12 65.9
Msa0611550 AT4G21940.1 31.111 135 87 3 58 189 407 538 1.35e-12 65.9
Msa0611550 AT3G51850.1 27.439 164 118 1 27 189 329 492 1.42e-12 65.9
Msa0611550 AT2G17290.1 31.618 136 84 4 58 189 391 521 1.67e-12 65.9
Msa0611550 AT2G17290.2 31.618 136 84 4 58 189 391 521 1.67e-12 65.9
Msa0611550 AT4G04710.1 31.579 133 89 2 58 189 351 482 1.84e-12 65.5
Msa0611550 AT1G74740.1 31.429 140 92 2 54 189 358 497 4.01e-12 64.7
Msa0611550 AT3G20410.2 29.630 135 89 3 58 189 397 528 7.79e-12 63.9
Msa0611550 AT3G20410.1 29.630 135 89 3 58 189 397 528 7.79e-12 63.9
Msa0611550 AT4G04740.4 28.148 135 91 3 58 189 374 505 2.62e-11 62.0
Msa0611550 AT4G04740.6 28.148 135 91 3 58 189 374 505 2.62e-11 62.0
Msa0611550 AT4G04740.5 28.148 135 91 3 58 189 374 505 2.62e-11 62.0
Msa0611550 AT4G04740.3 28.148 135 91 3 58 189 374 505 2.62e-11 62.0
Msa0611550 AT4G04740.1 28.148 135 91 3 58 189 374 505 2.62e-11 62.0
Msa0611550 AT4G04740.7 28.148 135 91 3 58 189 374 505 2.62e-11 62.0
Msa0611550 AT4G04710.4 31.852 135 88 3 58 189 351 484 2.63e-11 62.0
Msa0611550 AT2G38910.1 28.409 176 117 4 19 189 399 570 2.76e-11 62.0
Msa0611550 AT5G19450.2 34.559 136 83 3 58 189 363 496 2.85e-11 62.0
Msa0611550 AT5G19450.1 34.559 136 83 3 58 189 363 496 2.85e-11 62.0
Msa0611550 AT3G57530.1 34.351 131 80 3 62 189 373 500 6.04e-11 61.2
Msa0611550 AT1G61950.1 30.216 139 89 3 55 189 402 536 8.15e-11 60.8

Find 51 sgRNAs with CRISPR-Local

Find 55 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATCAATTTCGAAGAATTTA+TGG 0.163356 4_2:+59760718 Msa0611550:CDS
TCTCCATGCCTAGAGATTTC+AGG 0.242652 4_2:-59760637 None:intergenic
AGAAGAAGGGTGGCGGGATT+AGG 0.344511 4_2:+59760747 Msa0611550:CDS
TTCTCTTTGAGAGGTTGTAT+TGG 0.359751 4_2:-59760421 None:intergenic
TAAAGAAATGTTGTGGAAGC+TGG 0.364619 4_2:+59760841 Msa0611550:CDS
ATCTCTAGGCATGGAGAAAT+CGG 0.392953 4_2:+59760643 Msa0611550:CDS
AAATTTGATTCCAATAAAGA+TGG 0.423505 4_2:+59760584 Msa0611550:CDS
AGATGGGAAAATATCTCAAC+AGG 0.423802 4_2:+59760601 Msa0611550:CDS
TTTCGAACATTTGATAAGAA+TGG 0.431044 4_2:+59760791 Msa0611550:CDS
CTCCATGCCTAGAGATTTCA+GGG 0.434046 4_2:-59760636 None:intergenic
ATTTATGGAGGCACAGAAGA+AGG 0.474383 4_2:+59760733 Msa0611550:CDS
TCAAAGGACAAACAGAACTC+TGG 0.476930 4_2:+59760473 Msa0611550:CDS
TTTCGGGTGGTTGACTTGGA+TGG 0.482059 4_2:+59760686 Msa0611550:CDS
GTGGTTGACTTGGATGGAGA+TGG 0.497520 4_2:+59760692 Msa0611550:CDS
TCAGGTACTTTCTCTTTGAG+AGG 0.501193 4_2:-59760430 None:intergenic
CAAAGGACAAACAGAACTCT+GGG 0.507659 4_2:+59760474 Msa0611550:CDS
ACATTTGATAAGAATGGTGA+TGG 0.510358 4_2:+59760797 Msa0611550:CDS
AGAGTCGTGACGGCTTCCTC+AGG 0.512598 4_2:-59760448 None:intergenic
GGTGCAGCCTTGAGGACTGC+AGG 0.514632 4_2:+59760870 Msa0611550:CDS
TCAAAGAACAGACAAAACTC+TGG 0.529354 4_2:+59760509 Msa0611550:CDS
GAGGCACAGAAGAAGGGTGG+CGG 0.540486 4_2:+59760740 Msa0611550:CDS
CAGCATTCAAACATGTCTCA+TGG 0.541379 4_2:-59760969 None:intergenic
TCTCATGGATTGAGTCATCA+TGG 0.547313 4_2:-59760954 None:intergenic
AGAGGTTGTATTGGAAATCG+AGG 0.549337 4_2:-59760412 None:intergenic
AAAGCTACCCTGAAATCTCT+AGG 0.549647 4_2:+59760629 Msa0611550:CDS
AGGCACAGAAGAAGGGTGGC+GGG 0.550603 4_2:+59760741 Msa0611550:CDS
AATTTGATTCCAATAAAGAT+GGG 0.558831 4_2:+59760585 Msa0611550:CDS
TCCTTTGAGAAGAGTCGTGA+CGG 0.561303 4_2:-59760458 None:intergenic
CCATTCGCCTGCAGTCCTCA+AGG 0.565957 4_2:-59760877 None:intergenic
GGGTGGCGGGATTAGGTCGT+TGG 0.568531 4_2:+59760754 Msa0611550:CDS
CCTTGAGGACTGCAGGCGAA+TGG 0.569437 4_2:+59760877 Msa0611550:CDS
AAGAAATTAAAGAAATGTTG+TGG 0.574845 4_2:+59760834 Msa0611550:CDS
CAAAGAACAGACAAAACTCT+GGG 0.588758 4_2:+59760510 Msa0611550:CDS
TACCCTGAAATCTCTAGGCA+TGG 0.589415 4_2:+59760634 Msa0611550:CDS
TGTTGTGGAAGCTGGAAGAA+AGG 0.589500 4_2:+59760849 Msa0611550:CDS
ACACCATCTTCATCTCATCA+AGG 0.589556 4_2:-59760556 None:intergenic
GAAGCTCTCATAAAATGTCA+AGG 0.593326 4_2:+59760381 None:intergenic
ATCTTCATCTCATCAAGGCT+AGG 0.593507 4_2:-59760551 None:intergenic
GGAAGAAAGGTGCAGCCTTG+AGG 0.598195 4_2:+59760862 Msa0611550:CDS
CAGGCGAATGGTGAGAGCAG+TGG 0.606356 4_2:+59760889 Msa0611550:CDS
TTTATGGAGGCACAGAAGAA+GGG 0.608726 4_2:+59760734 Msa0611550:CDS
GTGAGAGCAGTGGACACTGA+TGG 0.611578 4_2:+59760899 Msa0611550:CDS
GCAGTGGACACTGATGGAGA+TGG 0.626893 4_2:+59760905 Msa0611550:CDS
GCCGTCACGACTCTTCTCAA+AGG 0.629350 4_2:+59760457 Msa0611550:CDS
CAATTTCGAAGAATTTATGG+AGG 0.644323 4_2:+59760721 Msa0611550:CDS
CATTTGATAAGAATGGTGAT+GGG 0.644649 4_2:+59760798 Msa0611550:CDS
TAGCCTTGATGAGATGAAGA+TGG 0.657485 4_2:+59760553 Msa0611550:CDS
GGACACTGATGGAGATGGCA+TGG 0.659167 4_2:+59760910 Msa0611550:CDS
TCTCAAAGAGAAAGTACCTG+AGG 0.659784 4_2:+59760432 Msa0611550:CDS
ATGGAGGCACAGAAGAAGGG+TGG 0.680107 4_2:+59760737 Msa0611550:CDS
AAAGGACAAACAGAACTCTG+GGG 0.762759 4_2:+59760475 Msa0611550:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTTGATTCCAATAAAGA+TGG + chr4_2:59760584-59760603 Msa0611550:CDS 20.0%
!! AATTTGATTCCAATAAAGAT+GGG + chr4_2:59760585-59760604 Msa0611550:CDS 20.0%
!! AAGAAATTAAAGAAATGTTG+TGG + chr4_2:59760834-59760853 Msa0611550:CDS 20.0%
!! AGATATTTTCCCATCTTTAT+TGG - chr4_2:59760597-59760616 None:intergenic 25.0%
! CATCAATTTCGAAGAATTTA+TGG + chr4_2:59760718-59760737 Msa0611550:CDS 25.0%
! TTTCGAACATTTGATAAGAA+TGG + chr4_2:59760791-59760810 Msa0611550:CDS 25.0%
!! ATGAAGTGCCACATATTTTT+CGG + chr4_2:59760669-59760688 Msa0611550:CDS 30.0%
CAATTTCGAAGAATTTATGG+AGG + chr4_2:59760721-59760740 Msa0611550:CDS 30.0%
ACATTTGATAAGAATGGTGA+TGG + chr4_2:59760797-59760816 Msa0611550:CDS 30.0%
CATTTGATAAGAATGGTGAT+GGG + chr4_2:59760798-59760817 Msa0611550:CDS 30.0%
! TTCTCTTTGAGAGGTTGTAT+TGG - chr4_2:59760424-59760443 None:intergenic 35.0%
TCAAAGAACAGACAAAACTC+TGG + chr4_2:59760509-59760528 Msa0611550:CDS 35.0%
CAAAGAACAGACAAAACTCT+GGG + chr4_2:59760510-59760529 Msa0611550:CDS 35.0%
AGATGGGAAAATATCTCAAC+AGG + chr4_2:59760601-59760620 Msa0611550:CDS 35.0%
!! TGAAGTGCCACATATTTTTC+GGG + chr4_2:59760670-59760689 Msa0611550:CDS 35.0%
TAAAGAAATGTTGTGGAAGC+TGG + chr4_2:59760841-59760860 Msa0611550:CDS 35.0%
AGAGGTTGTATTGGAAATCG+AGG - chr4_2:59760415-59760434 None:intergenic 40.0%
TCAGGTACTTTCTCTTTGAG+AGG - chr4_2:59760433-59760452 None:intergenic 40.0%
!! TCTCAAAGAGAAAGTACCTG+AGG + chr4_2:59760432-59760451 Msa0611550:CDS 40.0%
TCAAAGGACAAACAGAACTC+TGG + chr4_2:59760473-59760492 Msa0611550:CDS 40.0%
CAAAGGACAAACAGAACTCT+GGG + chr4_2:59760474-59760493 Msa0611550:CDS 40.0%
AAAGGACAAACAGAACTCTG+GGG + chr4_2:59760475-59760494 Msa0611550:CDS 40.0%
ATCTTCATCTCATCAAGGCT+AGG - chr4_2:59760554-59760573 None:intergenic 40.0%
! TAGCCTTGATGAGATGAAGA+TGG + chr4_2:59760553-59760572 Msa0611550:CDS 40.0%
ACACCATCTTCATCTCATCA+AGG - chr4_2:59760559-59760578 None:intergenic 40.0%
AAAGCTACCCTGAAATCTCT+AGG + chr4_2:59760629-59760648 Msa0611550:CDS 40.0%
ATCTCTAGGCATGGAGAAAT+CGG + chr4_2:59760643-59760662 Msa0611550:CDS 40.0%
TCAACCACCCGAAAAATATG+TGG - chr4_2:59760680-59760699 None:intergenic 40.0%
!!! TATTTTTCGGGTGGTTGACT+TGG + chr4_2:59760682-59760701 Msa0611550:CDS 40.0%
! ATTTATGGAGGCACAGAAGA+AGG + chr4_2:59760733-59760752 Msa0611550:CDS 40.0%
! TTTATGGAGGCACAGAAGAA+GGG + chr4_2:59760734-59760753 Msa0611550:CDS 40.0%
! TCTCATGGATTGAGTCATCA+TGG - chr4_2:59760957-59760976 None:intergenic 40.0%
! TCCTTTGAGAAGAGTCGTGA+CGG - chr4_2:59760461-59760480 None:intergenic 45.0%
TACCCTGAAATCTCTAGGCA+TGG + chr4_2:59760634-59760653 Msa0611550:CDS 45.0%
CTCCATGCCTAGAGATTTCA+GGG - chr4_2:59760639-59760658 None:intergenic 45.0%
TCTCCATGCCTAGAGATTTC+AGG - chr4_2:59760640-59760659 None:intergenic 45.0%
!!! AGTGCCACATATTTTTCGGG+TGG + chr4_2:59760673-59760692 Msa0611550:CDS 45.0%
TGTTGTGGAAGCTGGAAGAA+AGG + chr4_2:59760849-59760868 Msa0611550:CDS 45.0%
!! TTTCGGGTGGTTGACTTGGA+TGG + chr4_2:59760686-59760705 Msa0611550:CDS 50.0%
!! GTGGTTGACTTGGATGGAGA+TGG + chr4_2:59760692-59760711 Msa0611550:CDS 50.0%
GCCGTCACGACTCTTCTCAA+AGG + chr4_2:59760457-59760476 Msa0611550:CDS 55.0%
! ATGGAGGCACAGAAGAAGGG+TGG + chr4_2:59760737-59760756 Msa0611550:CDS 55.0%
!! AGAAGAAGGGTGGCGGGATT+AGG + chr4_2:59760747-59760766 Msa0611550:CDS 55.0%
! GGAAGAAAGGTGCAGCCTTG+AGG + chr4_2:59760862-59760881 Msa0611550:CDS 55.0%
GTGAGAGCAGTGGACACTGA+TGG + chr4_2:59760899-59760918 Msa0611550:CDS 55.0%
GCAGTGGACACTGATGGAGA+TGG + chr4_2:59760905-59760924 Msa0611550:CDS 55.0%
GGACACTGATGGAGATGGCA+TGG + chr4_2:59760910-59760929 Msa0611550:CDS 55.0%
AGAGTCGTGACGGCTTCCTC+AGG - chr4_2:59760451-59760470 None:intergenic 60.0%
GAGGCACAGAAGAAGGGTGG+CGG + chr4_2:59760740-59760759 Msa0611550:CDS 60.0%
AGGCACAGAAGAAGGGTGGC+GGG + chr4_2:59760741-59760760 Msa0611550:CDS 60.0%
CCATTCGCCTGCAGTCCTCA+AGG - chr4_2:59760880-59760899 None:intergenic 60.0%
CCTTGAGGACTGCAGGCGAA+TGG + chr4_2:59760877-59760896 Msa0611550:CDS 60.0%
CAGGCGAATGGTGAGAGCAG+TGG + chr4_2:59760889-59760908 Msa0611550:CDS 60.0%
!! GGGTGGCGGGATTAGGTCGT+TGG + chr4_2:59760754-59760773 Msa0611550:CDS 65.0%
GGTGCAGCCTTGAGGACTGC+AGG + chr4_2:59760870-59760889 Msa0611550:CDS 65.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 59760395 59760985 59760395 ID=Msa0611550;Name=Msa0611550
chr4_2 mRNA 59760395 59760985 59760395 ID=Msa0611550-mRNA-1;Parent=Msa0611550;Name=Msa0611550-mRNA-1;_AED=0.33;_eAED=0.33;_QI=0|-1|0|1|-1|1|1|0|196
chr4_2 exon 59760395 59760985 59760395 ID=Msa0611550-mRNA-1:exon:15896;Parent=Msa0611550-mRNA-1
chr4_2 CDS 59760395 59760985 59760395 ID=Msa0611550-mRNA-1:cds;Parent=Msa0611550-mRNA-1
Gene Sequence

>Msa0611550

ATGTCAAGGACAAGTTTCCTCGATTTCCAATACAACCTCTCAAAGAGAAAGTACCTGAGGAAGCCGTCACGACTCTTCTCAAAGGACAAACAGAACTCTGGGGTAACTATGTTCTCAAAGAACAGACAAAACTCTGGGCTAAAATATATTTTTCAGCCTAGCCTTGATGAGATGAAGATGGTGTTTGACAAATTTGATTCCAATAAAGATGGGAAAATATCTCAACAGGAGTACAAAGCTACCCTGAAATCTCTAGGCATGGAGAAATCGGTGAATGAAGTGCCACATATTTTTCGGGTGGTTGACTTGGATGGAGATGGATTCATCAATTTCGAAGAATTTATGGAGGCACAGAAGAAGGGTGGCGGGATTAGGTCGTTGGATATACAGACTGCGTTTCGAACATTTGATAAGAATGGTGATGGGAAAATAAGTGCTGAAGAAATTAAAGAAATGTTGTGGAAGCTGGAAGAAAGGTGCAGCCTTGAGGACTGCAGGCGAATGGTGAGAGCAGTGGACACTGATGGAGATGGCATGGTTGACATGAACGAGTTTGTAGCCATGATGACTCAATCCATGAGACATGTTTGA
Protein sequence

>Msa0611550

MSRTSFLDFQYNLSKRKYLRKPSRLFSKDKQNSGVTMFSKNRQNSGLKYIFQPSLDEMKMVFDKFDSNKDGKISQQEYKATLKSLGMEKSVNEVPHIFRVVDLDGDGFINFEEFMEAQKKGGGIRSLDIQTAFRTFDKNGDGKISAEEIKEMLWKLEERCSLEDCRRMVRAVDTDGDGMVDMNEFVAMMTQSMRHV*