AlfalfaGEDB | Msa1199610

M. sativa cultivar zhongmu-4 / Msa1199610

KEGG: -
GO: -
PFAM: PF00069.28

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1199610	sp\|Q8GZ99\|HPCA1_ARATH	60.331	242	79	5	236	476	728	953	1.38e-83	280
Msa1199610	sp\|Q8GZ99\|HPCA1_ARATH	41.117	197	109	5	37	227	441	636	4.62e-27	118

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1199610	A0A2P5CVC0	80.549	437	83	2	40	476	448	882	0.0	684

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0061590	Msa1199610	-0.818934	1.512444e-52	-8.615850e-47
Msa0091470	Msa1199610	0.826403	2.765742e-54	-8.615850e-47
Msa0199740	Msa1199610	0.805210	1.489942e-49	-8.615850e-47
Msa0270840	Msa1199610	0.811729	6.050687e-51	-8.615850e-47
Msa0405250	Msa1199610	-0.833099	6.476761e-56	-8.615850e-47
Msa0646340	Msa1199610	0.808610	2.845763e-50	-8.615850e-47
Msa0675010	Msa1199610	0.838989	2.071803e-57	-8.615850e-47
Msa0698650	Msa1199610	0.802794	4.736883e-49	-8.615850e-47
Msa0707040	Msa1199610	0.815721	7.994706e-52	-8.615850e-47
Msa0744690	Msa1199610	0.813919	2.005470e-51	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1199610	MtrunA17_Chr8g0369211	88.971	272	30	0	205	476	695	966	2.46e-152	458
Msa1199610	MtrunA17_Chr8g0369211	97.938	194	4	0	42	235	449	642	4.61e-132	405
Msa1199610	MtrunA17_Chr8g0369211	90.909	44	2	1	1	44	103	144	5.12e-15	77.8
Msa1199610	MtrunA17_Chr8g0369231	82.721	272	47	0	205	476	342	613	2.24e-143	424
Msa1199610	MtrunA17_Chr8g0369231	88.542	192	22	0	44	235	98	289	2.82e-119	362
Msa1199610	MtrunA17_Chr2g0283111	56.017	241	92	4	236	476	708	934	1.61e-80	268
Msa1199610	MtrunA17_Chr2g0283111	38.182	220	114	7	24	235	419	624	1.11e-28	120
Msa1199610	MtrunA17_Chr2g0291501	60.748	214	80	3	236	449	736	945	9.96e-79	263
Msa1199610	MtrunA17_Chr3g0108361	47.639	233	101	6	237	455	720	945	1.49e-49	182
Msa1199610	MtrunA17_Chr3g0108351	52.525	198	79	5	237	425	718	909	5.79e-48	177

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1199610	AT1G79620.4	73.980	196	51	0	40	235	482	677	3.45e-99	312
Msa1199610	AT1G79620.3	73.980	196	51	0	40	235	456	651	8.42e-99	313
Msa1199610	AT1G79620.2	73.980	196	51	0	40	235	456	651	2.73e-98	313
Msa1199610	AT1G79620.2	91.429	70	6	0	235	304	734	803	4.90e-33	133
Msa1199610	AT1G79620.1	73.980	196	51	0	40	235	456	651	2.67e-97	314
Msa1199610	AT1G79620.1	66.939	245	71	4	235	476	734	971	2.47e-95	309
Msa1199610	AT1G79620.5	73.980	196	51	0	40	235	482	677	4.75e-97	314
Msa1199610	AT1G79620.5	66.939	245	71	4	235	476	760	997	3.84e-95	309
Msa1199610	AT5G49760.1	60.331	242	79	5	236	476	728	953	1.40e-84	280
Msa1199610	AT5G49760.1	41.117	197	109	5	37	227	441	636	4.69e-28	118

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Find 190 sgRNAs with CRISPR-Local

Find 1316 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATTACCATGTGCCAGAG+TGG	0.654835	8_1:-51822556	Msa1199610:five_prime_UTR
AAAATTTATATAATCTATTT+GGG	0.323991	8_1:+51802751	None:intergenic
AAACTATAGGCCACAGAAAT+AGG	0.242060	8_1:+51802624	None:intergenic
AACTCAAGTTAAAGGGACAA+TGG	0.589377	8_1:-51803873	Msa1199610:intron
AAGAGTGACGTATACAGTTT+CGG	0.401785	8_1:-51803216	Msa1199610:CDS
AAGCGTGCAGAGAGAGCAAT+TGG	0.390058	8_1:-51805388	Msa1199610:CDS
AAGGGAACTAATGGATCCAG+TGG	0.645637	8_1:-51803082	Msa1199610:CDS
AAGGTAACAATTCGTCCCAA+TGG	0.574060	8_1:+51816181	None:intergenic
AAGTGCACCAACAATTTCTC+TGG	0.440317	8_1:-51805184	Msa1199610:CDS
AATCCATACAAGAAAACTAT+AGG	0.429958	8_1:+51802611	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TGTTATCTAAATTACCACTC+TGG	+	chr8_1:51803101-51803120	None:intergenic	30.0%
ATGTATTAGGTAACAGTGAA+TGG	+	chr8_1:51803166-51803185	None:intergenic	30.0%
ATGGCCATAAATATGAAAGA+AGG	+	chr8_1:51803232-51803251	None:intergenic	30.0%
TGAACAGAAAAGAAACAAGA+GGG	+	chr8_1:51803274-51803293	None:intergenic	30.0%
TTCTAATGACAAAGTATGTG+AGG	-	chr8_1:51803916-51803935	Msa1199610:CDS	30.0%
AATAACTCAAAATGTAAGCG+TGG	+	chr8_1:51803971-51803990	None:intergenic	30.0%
ATCCATGAATGTTCAATTGT+GGG	+	chr8_1:51804021-51804040	None:intergenic	30.0%
TATCCATGAATGTTCAATTG+TGG	+	chr8_1:51804022-51804041	None:intergenic	30.0%
CCACAATCAACAAAATCATT+TGG	+	chr8_1:51804047-51804066	None:intergenic	30.0%
AAGATTAGAACATCGAGAAA+AGG	-	chr8_1:51804218-51804237	Msa1199610:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_1	gene	51802440	51823222	51802440	ID=Msa1199610;Name=Msa1199610
chr8_1	mRNA	51802440	51823222	51802440	ID=Msa1199610-mRNA-1;Parent=Msa1199610;Name=Msa1199610-mRNA-1;_AED=0.33;_eAED=0.33;_QI=1166\|0.72\|0.66\|1\|0.81\|0.75\|12\|325\|476
chr8_1	exon	51802440	51803283	51802440	ID=Msa1199610-mRNA-1:exon:16406;Parent=Msa1199610-mRNA-1
chr8_1	exon	51803874	51804080	51803874	ID=Msa1199610-mRNA-1:exon:16405;Parent=Msa1199610-mRNA-1
chr8_1	exon	51805150	51805289	51805150	ID=Msa1199610-mRNA-1:exon:16404;Parent=Msa1199610-mRNA-1
chr8_1	exon	51805371	51805523	51805371	ID=Msa1199610-mRNA-1:exon:16403;Parent=Msa1199610-mRNA-1
chr8_1	exon	51805774	51806057	51805774	ID=Msa1199610-mRNA-1:exon:16402;Parent=Msa1199610-mRNA-1
chr8_1	exon	51806308	51806385	51806308	ID=Msa1199610-mRNA-1:exon:16401;Parent=Msa1199610-mRNA-1
chr8_1	exon	51806481	51806562	51806481	ID=Msa1199610-mRNA-1:exon:16400;Parent=Msa1199610-mRNA-1
chr8_1	exon	51812787	51812884	51812787	ID=Msa1199610-mRNA-1:exon:16399;Parent=Msa1199610-mRNA-1
chr8_1	exon	51813782	51813843	51813782	ID=Msa1199610-mRNA-1:exon:16398;Parent=Msa1199610-mRNA-1
chr8_1	exon	51814020	51814087	51814020	ID=Msa1199610-mRNA-1:exon:16397;Parent=Msa1199610-mRNA-1
chr8_1	exon	51816177	51816403	51816177	ID=Msa1199610-mRNA-1:exon:16396;Parent=Msa1199610-mRNA-1
chr8_1	exon	51822544	51823222	51822544	ID=Msa1199610-mRNA-1:exon:16395;Parent=Msa1199610-mRNA-1
chr8_1	five_prime_UTR	51822544	51823222	51822544	ID=Msa1199610-mRNA-1:five_prime_utr;Parent=Msa1199610-mRNA-1
chr8_1	five_prime_UTR	51816177	51816403	51816177	ID=Msa1199610-mRNA-1:five_prime_utr;Parent=Msa1199610-mRNA-1
chr8_1	five_prime_UTR	51814020	51814087	51814020	ID=Msa1199610-mRNA-1:five_prime_utr;Parent=Msa1199610-mRNA-1
chr8_1	five_prime_UTR	51813782	51813843	51813782	ID=Msa1199610-mRNA-1:five_prime_utr;Parent=Msa1199610-mRNA-1
chr8_1	five_prime_UTR	51812787	51812884	51812787	ID=Msa1199610-mRNA-1:five_prime_utr;Parent=Msa1199610-mRNA-1
chr8_1	five_prime_UTR	51806531	51806562	51806531	ID=Msa1199610-mRNA-1:five_prime_utr;Parent=Msa1199610-mRNA-1
chr8_1	CDS	51806481	51806530	51806481	ID=Msa1199610-mRNA-1:cds;Parent=Msa1199610-mRNA-1
chr8_1	CDS	51806308	51806385	51806308	ID=Msa1199610-mRNA-1:cds;Parent=Msa1199610-mRNA-1
chr8_1	CDS	51805774	51806057	51805774	ID=Msa1199610-mRNA-1:cds;Parent=Msa1199610-mRNA-1
chr8_1	CDS	51805371	51805523	51805371	ID=Msa1199610-mRNA-1:cds;Parent=Msa1199610-mRNA-1
chr8_1	CDS	51805150	51805289	51805150	ID=Msa1199610-mRNA-1:cds;Parent=Msa1199610-mRNA-1
chr8_1	CDS	51803874	51804080	51803874	ID=Msa1199610-mRNA-1:cds;Parent=Msa1199610-mRNA-1
chr8_1	CDS	51802765	51803283	51802765	ID=Msa1199610-mRNA-1:cds;Parent=Msa1199610-mRNA-1
chr8_1	three_prime_UTR	51802440	51802764	51802440	ID=Msa1199610-mRNA-1:three_prime_utr;Parent=Msa1199610-mRNA-1

Gene Sequence

>Msa1199610

ATGGGTTCTATCTCTCCAGAATTGGGAGACCTAAGCAAGCTAAATATTCTGATTTTGGCTGGTTCTGGTTGTAGCTTCAGTGGTAATATTCCAGATAAATTGGGGGACCTTTCAGAGCTAGCTTTCTTAGCACCTTTGTTCAGAGAATTGTCTAATATAACTATTTTCCATTCGCTTGAAATGAGTTTATGGGTGAAGTTAGGTTTAACACCTGGCTCAGTTTCTTTGCAAAACCCTTTTTTCAACGGTGACGATTATCTTCAAATGCAACTAGCACTCTTTCCACCAACAGGACAATATTTTAACAGGACAGAAGTTCAAAGAATTGGATTTTCGTTGAGTAATCAAACATATAAGCCTCCACATGAATTTGGACCCTATTACTTCATTGCATTTCCTTATGCTTTTCCAGATTCCCACGGAGGATTTTCTCTTAGCAGAGGTGTTATTATTGGGATAGCAGCTGTGAGCACATTTGTGGTCCTTTGCCTCATAGGGTTAGCTGTATATGCTGTCCTGCAAAAGAAGCGTGCAGAGAGAGCAATTGGAATAAGTAGACCATTTGCATCCTGGGCACCAAGTGGCAAGGACAGTGGAGGTGCACCACAATTGAAAGGAGCAAGATGGTTCTCCTATGATGAACTCAAAAAGTGCACCAACAATTTCTCTGGAAGCAATGAACTAGGTTTCGGTGGCTATGGCAAGAGGAGACTCCGTATTGCTCTTGGCTCAGCTAGAGGACTTGCATACTTACATGAACTTGCCAACCCTCCCATAATCCACAGAGATGTGAAGTCCACCAATATCTTACTGGACGAAAGTTTGACAGCAAAGGTTGCAGATTTTGGCCTCTCTAAGCTTGTATCAGACAGCGAGAAAGGTCATGTTTCAACTCAAGTTAAAGGGACAATGGGCTATCTAGATCCTGAATATTACATGACTCAACAACTAACCGAAAAGAGTGACGTATACAGTTTCGGGGTAGTTATGCTTGAACTGATAACATCCAAACAGCCAATCAAAAAGGGTAAGTACGTCGTTCGCGAAGTTCGAACCTTAATGAACGACAAAGATGAAGAGTATTATGGTTTAAGGGAACTAATGGATCCAGTGGTGAGAAACACAGCCAACCTGATAGGATTTGGGAGGTTCTTAGAGTTGGCAATGCAATGTGTTGAGGAATTGGCTTCAGACCGACCAACAATGAGTGAAGTTGTGAAAGCGTTAGAAACCATATTACAAAATGATGGAATTAATACAAATTCCACTTCTGCTTCATCATCTGCCACTGACTTTAATGCTTCTAAGGGTGCTGCAATAAGACATCCTTATATTGATAATACTTTTACTAAGAAGGATGATGTTATTGAGAGTAATGCCTTTGATTACAGTGGAGGGTACACACTTTCAACAAAAGTGGAACCCAAATAG

Protein sequence

>Msa1199610

MGSISPELGDLSKLNILILAGSGCSFSGNIPDKLGDLSELAFLAPLFRELSNITIFHSLEMSLWVKLGLTPGSVSLQNPFFNGDDYLQMQLALFPPTGQYFNRTEVQRIGFSLSNQTYKPPHEFGPYYFIAFPYAFPDSHGGFSLSRGVIIGIAAVSTFVVLCLIGLAVYAVLQKKRAERAIGISRPFASWAPSGKDSGGAPQLKGARWFSYDELKKCTNNFSGSNELGFGGYGKRRLRIALGSARGLAYLHELANPPIIHRDVKSTNILLDESLTAKVADFGLSKLVSDSEKGHVSTQVKGTMGYLDPEYYMTQQLTEKSDVYSFGVVMLELITSKQPIKKGKYVVREVRTLMNDKDEEYYGLRELMDPVVRNTANLIGFGRFLELAMQCVEELASDRPTMSEVVKALETILQNDGINTNSTSASSSATDFNASKGAAIRHPYIDNTFTKKDDVIESNAFDYSGGYTLSTKVEPK*