AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1182800

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1182800 MtrunA17_Chr8g0338511 96.729 428 14 0 127 554 25 452 0.0 862
Msa1182800 MtrunA17_Chr8g0338511 44.910 167 34 2 22 130 28 194 4.70e-29 120
Msa1182800 MtrunA17_Chr8g0338471 79.376 417 81 2 130 545 23 435 0.0 694
Msa1182800 MtrunA17_Chr8g0338471 41.954 174 38 4 16 130 17 186 5.70e-24 106
Msa1182800 MtrunA17_Chr8g0338501 76.291 426 96 2 130 554 52 473 0.0 676
Msa1182800 MtrunA17_Chr8g0338501 39.766 171 40 4 19 130 49 215 3.04e-20 95.1
Msa1182800 MtrunA17_Chr8g0338521 71.148 357 92 5 149 502 12 360 3.55e-174 496
Msa1182800 MtrunA17_Chr8g0338521 57.895 95 31 3 45 130 67 161 1.08e-20 94.0
Msa1182800 MtrunA17_Chr8g0338531 67.324 355 93 4 195 542 8 346 3.52e-157 467
Msa1182800 MtrunA17_Chr8g0338531 51.765 85 34 1 53 130 25 109 4.88e-16 81.6
Msa1182800 MtrunA17_Chr2g0311541 43.675 419 231 5 135 550 18 434 1.76e-117 365
Msa1182800 MtrunA17_Chr2g0311551 44.175 412 225 5 135 545 259 666 1.85e-117 371
Msa1182800 MtrunA17_Chr8g0338481 67.364 239 53 1 300 538 1 214 2.49e-112 335
Msa1182800 MtrunA17_Chr2g0311521 43.165 417 232 5 136 550 261 674 4.60e-111 355
Msa1182800 MtrunA17_Chr5g0399591 41.404 413 216 12 153 549 40 442 7.77e-100 320
Msa1182800 MtrunA17_Chr5g0399561 39.252 428 233 13 143 554 32 448 1.38e-94 306
Msa1182800 MtrunA17_Chr2g0311511 43.077 325 173 8 135 449 35 357 5.59e-79 263
Msa1182800 MtrunA17_Chr5g0394381 32.710 428 249 19 136 553 24 422 3.56e-46 173
Msa1182800 MtrunA17_Chr4g0063701 30.732 410 242 17 149 539 51 437 1.52e-39 154
Msa1182800 MtrunA17_Chr4g0063691 30.414 411 246 17 136 525 37 428 2.20e-33 135
Msa1182800 MtrunA17_Chr0c04g0492001 28.471 425 270 16 139 551 29 431 7.11e-32 130
Msa1182800 MtrunA17_Chr3g0134291 28.471 425 270 16 139 551 29 431 2.29e-31 129
Msa1182800 MtrunA17_Chr0c04g0492031 28.471 425 270 16 139 551 29 431 2.47e-31 129
Msa1182800 MtrunA17_Chr3g0106201 27.619 420 268 12 141 547 24 420 6.75e-31 127
Msa1182800 MtrunA17_Chr3g0106141 28.605 423 269 13 141 550 24 426 1.78e-30 126
Msa1182800 MtrunA17_Chr3g0106121 28.744 414 260 12 141 541 24 415 3.23e-30 125
Msa1182800 MtrunA17_Chr3g0106131 28.837 430 263 12 141 554 24 426 1.10e-29 124
Msa1182800 MtrunA17_Chr3g0106151 28.571 413 242 15 152 547 38 414 5.29e-29 122
Msa1182800 MtrunA17_Chr3g0106211 29.111 371 236 12 141 503 27 378 1.67e-27 117
Msa1182800 MtrunA17_Chr7g0257821 28.205 390 233 16 153 531 45 398 2.53e-26 114
Msa1182800 MtrunA17_Chr3g0106221 27.614 373 226 10 152 504 37 385 7.38e-26 112
Msa1182800 MtrunA17_Chr1g0202331 25.556 450 274 19 134 544 24 451 1.43e-25 111
Msa1182800 MtrunA17_Chr3g0106191 28.883 367 219 13 190 547 2 335 5.16e-21 97.1
Msa1182800 MtrunA17_Chr7g0237341 26.044 407 258 16 152 543 43 421 4.86e-20 94.4
Msa1182800 MtrunA17_Chr2g0323301 25.926 405 235 17 130 505 24 392 1.12e-19 93.2
Msa1182800 MtrunA17_Chr4g0053161 24.709 429 273 17 134 543 44 441 1.73e-19 92.4
Msa1182800 MtrunA17_Chr7g0237331 24.631 406 261 12 152 541 44 420 5.31e-19 90.9
Msa1182800 MtrunA17_Chr7g0237351 24.027 437 289 16 134 554 25 434 7.31e-19 90.5
Msa1182800 MtrunA17_Chr4g0042351 26.171 363 228 14 154 503 42 377 8.96e-19 90.1
Msa1182800 MtrunA17_Chr4g0042391 25.895 363 233 12 152 503 40 377 1.03e-18 90.1
Msa1182800 MtrunA17_Chr3g0137741 27.717 368 217 18 151 509 52 379 7.79e-18 87.4
Msa1182800 MtrunA17_Chr8g0361311 27.513 378 216 17 152 504 39 383 7.37e-17 83.2
Msa1182800 MtrunA17_Chr2g0280701 26.385 379 219 19 152 504 38 382 1.13e-16 83.6
Msa1182800 MtrunA17_Chr4g0023071 23.896 385 236 14 152 508 42 397 1.93e-16 82.8
Msa1182800 MtrunA17_Chr8g0361251 27.154 383 211 17 152 504 39 383 3.87e-16 82.0
Msa1182800 MtrunA17_Chr3g0134321 33.766 154 95 4 152 300 38 189 6.41e-16 81.3
Msa1182800 MtrunA17_Chr2g0280751 25.185 405 251 16 130 514 28 400 7.58e-16 81.3
Msa1182800 MtrunA17_Chr4g0048691 27.763 389 229 18 152 513 61 424 1.02e-15 80.1
Msa1182800 MtrunA17_Chr2g0280681 24.934 377 222 16 152 503 43 383 1.31e-15 80.5
Msa1182800 MtrunA17_Chr8g0361301 25.806 372 229 14 152 504 40 383 1.76e-15 79.7
Msa1182800 MtrunA17_Chr4g0042381 24.863 366 234 13 152 503 39 377 2.61e-15 79.3
Msa1182800 MtrunA17_Chr2g0280801 23.409 440 266 18 153 553 45 452 3.73e-15 79.0
Msa1182800 MtrunA17_Chr2g0280691 26.221 389 232 18 140 505 27 383 3.92e-15 79.0
Msa1182800 MtrunA17_Chr3g0084621 25.132 378 225 17 152 505 44 387 4.18e-15 78.6
Msa1182800 MtrunA17_Chr2g0280731 28.205 312 184 12 152 446 46 334 6.22e-15 78.2
Msa1182800 MtrunA17_Chr3g0098791 23.153 406 253 15 122 505 18 386 6.65e-15 77.8
Msa1182800 MtrunA17_Chr5g0428111 26.471 374 233 17 152 508 45 393 2.07e-14 76.6
Msa1182800 MtrunA17_Chr8g0361261 24.821 419 267 15 106 504 24 414 2.40e-14 76.3
Msa1182800 MtrunA17_Chr8g0361441 25.455 385 248 13 135 505 29 388 2.74e-14 76.3
Msa1182800 MtrunA17_Chr7g0236161 23.380 432 261 19 152 544 44 444 3.21e-14 75.9
Msa1182800 MtrunA17_Chr7g0236201 23.380 432 261 19 152 544 44 444 3.57e-14 75.9
Msa1182800 MtrunA17_Chr1g0149381 26.555 418 238 21 151 549 57 424 4.06e-14 75.5
Msa1182800 MtrunA17_Chr2g0280841 23.741 417 249 20 159 538 53 437 4.84e-14 74.3
Msa1182800 MtrunA17_Chr2g0318811 26.768 396 232 17 130 504 20 378 7.15e-14 74.7
Msa1182800 MtrunA17_Chr3g0078521 26.110 383 225 19 152 503 40 395 1.06e-13 74.3
Msa1182800 MtrunA17_Chr7g0236121 23.218 435 270 18 152 552 44 448 1.49e-13 73.6
Msa1182800 MtrunA17_Chr4g0048651 27.523 327 191 16 152 456 61 363 2.54e-13 72.4
Msa1182800 MtrunA17_Chr3g0093161 24.339 378 251 14 140 504 25 380 2.75e-13 72.8
Msa1182800 MtrunA17_Chr3g0084491 24.865 370 233 14 152 504 57 398 2.88e-13 72.8
Msa1182800 MtrunA17_Chr3g0084681 23.958 384 222 16 152 505 44 387 3.21e-13 72.8
Msa1182800 MtrunA17_Chr3g0084651 23.810 378 229 15 152 503 41 385 4.45e-13 71.6
Msa1182800 MtrunA17_Chr2g0318831 26.738 374 220 17 152 504 41 381 4.59e-13 72.0
Msa1182800 MtrunA17_Chr2g0280791 24.672 381 236 15 159 514 47 401 4.68e-13 72.0
Msa1182800 MtrunA17_Chr4g0048761 25.773 388 218 19 152 505 56 407 1.03e-12 70.9
Msa1182800 MtrunA17_Chr3g0078531 27.273 385 218 20 152 503 52 407 1.15e-12 70.9
Msa1182800 MtrunA17_Chr2g0323241 31.492 181 111 6 134 304 23 200 1.28e-12 70.1
Msa1182800 MtrunA17_Chr8g0340091 31.361 169 101 7 137 293 80 245 2.47e-12 69.7
Msa1182800 MtrunA17_Chr3g0084431 23.096 407 230 16 135 505 22 381 2.97e-12 69.7
Msa1182800 MtrunA17_Chr4g0042361 24.804 383 226 18 152 504 74 424 3.15e-12 69.7
Msa1182800 MtrunA17_Chr2g0318841 24.599 374 228 16 152 504 38 378 3.45e-12 69.3
Msa1182800 MtrunA17_Chr1g0205551 29.932 147 89 6 234 375 1 138 3.59e-12 63.9
Msa1182800 MtrunA17_Chr4g0042421 23.469 392 254 15 133 503 19 385 4.38e-12 68.9
Msa1182800 MtrunA17_Chr4g0048641 29.568 301 167 16 152 430 63 340 5.10e-12 68.9
Msa1182800 MtrunA17_Chr3g0106161 30.055 183 106 5 366 543 90 255 5.79e-12 68.6
Msa1182800 MtrunA17_Chr7g0237391 23.775 408 267 18 152 543 42 421 6.18e-12 68.6
Msa1182800 MtrunA17_Chr8g0340061 31.361 169 101 7 137 293 27 192 7.72e-12 67.8
Msa1182800 MtrunA17_Chr2g0333091 32.237 152 96 3 151 302 50 194 9.91e-12 67.8
Msa1182800 MtrunA17_Chr7g0236641 22.603 438 278 20 136 542 25 432 1.33e-11 67.4
Msa1182800 MtrunA17_Chr3g0078541 26.702 382 223 21 152 503 52 406 1.54e-11 67.4
Msa1182800 MtrunA17_Chr3g0084451 23.684 380 225 14 152 505 44 384 2.58e-11 66.6
Msa1182800 MtrunA17_Chr8g0361431 26.266 316 195 14 152 456 52 340 3.11e-11 66.2
Msa1182800 MtrunA17_Chr8g0340071 29.139 151 96 4 152 293 44 192 3.94e-11 65.9
Msa1182800 MtrunA17_Chr2g0315851 25.507 345 217 15 187 515 93 413 4.93e-11 65.1
Msa1182800 MtrunA17_Chr2g0315841 25.000 352 210 11 187 516 93 412 5.26e-11 65.1
Msa1182800 MtrunA17_Chr3g0114851 28.621 290 171 14 138 415 28 293 6.03e-11 65.5
Msa1182800 MtrunA17_Chr4g0048801 40.000 135 62 6 154 273 39 169 9.25e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1182800 AT5G24080.2 27.438 441 269 20 132 540 26 447 4.30e-29 122
Msa1182800 AT2G41890.1 27.344 384 243 14 134 508 24 380 1.18e-17 87.0
Msa1182800 AT3G12000.1 26.178 382 239 13 143 504 40 398 1.89e-17 85.5
Msa1182800 AT1G65790.3 24.682 393 239 13 134 505 28 384 3.58e-17 85.5
Msa1182800 AT1G65790.2 24.682 393 239 13 134 505 28 384 3.58e-17 85.5
Msa1182800 AT1G65790.1 24.936 393 238 13 134 505 28 384 4.41e-17 85.5
Msa1182800 AT1G34300.1 24.619 394 247 17 134 518 25 377 7.24e-17 84.7
Msa1182800 AT1G11330.1 25.897 390 246 15 147 514 42 410 1.36e-16 84.0
Msa1182800 AT1G11330.2 25.897 390 246 15 147 514 42 410 1.36e-16 84.0
Msa1182800 AT4G21390.2 24.941 421 261 15 125 519 18 409 1.67e-16 83.6
Msa1182800 AT1G11340.1 27.154 383 211 18 152 503 34 379 1.98e-16 83.2
Msa1182800 AT1G11330.3 25.897 390 246 15 147 514 42 410 2.01e-16 83.2
Msa1182800 AT4G21390.1 24.941 421 261 15 125 519 18 409 2.61e-16 83.2
Msa1182800 AT1G11340.3 27.415 383 210 19 152 503 102 447 3.26e-16 82.8
Msa1182800 AT1G11340.2 27.154 383 211 18 152 503 102 447 3.46e-16 82.4
Msa1182800 AT1G11410.3 24.865 370 236 16 152 503 13 358 5.53e-15 79.0
Msa1182800 AT1G11410.1 24.865 370 236 16 152 503 37 382 5.93e-15 78.6
Msa1182800 AT1G11410.4 24.865 370 236 16 152 503 44 389 6.61e-15 78.6
Msa1182800 AT1G11300.1 24.181 397 249 16 142 514 32 400 6.14e-14 75.5
Msa1182800 AT1G11300.2 24.181 397 249 16 142 514 32 400 6.46e-14 75.5
Msa1182800 AT4G21380.2 22.449 392 257 14 132 505 28 390 1.14e-13 74.3
Msa1182800 AT1G78850.1 23.607 377 247 15 162 515 52 410 1.59e-13 73.2
Msa1182800 AT1G78860.1 23.280 378 247 15 162 515 52 410 1.70e-13 73.2
Msa1182800 AT4G21380.1 22.449 392 257 14 132 505 28 390 2.30e-13 73.6
Msa1182800 AT1G65800.2 24.935 385 228 17 147 505 39 388 3.28e-13 72.8
Msa1182800 AT1G65800.1 24.935 385 228 17 147 505 39 388 4.89e-13 72.4
Msa1182800 AT1G11303.1 23.671 414 242 15 142 519 32 407 8.52e-13 71.6
Msa1182800 AT1G16905.1 24.259 371 215 16 170 515 69 398 3.68e-12 68.9
Msa1182800 AT4G03230.4 23.653 427 269 18 152 553 44 438 4.58e-12 69.7
Msa1182800 AT4G03230.1 23.653 427 269 18 152 553 44 438 4.58e-12 69.7
Msa1182800 AT4G03230.8 23.515 421 264 18 152 546 44 432 5.30e-12 69.3
Msa1182800 AT1G11350.1 26.253 419 218 21 141 514 26 398 1.26e-11 68.2
Msa1182800 AT1G61380.1 23.155 393 249 17 152 519 35 399 1.47e-11 67.8
Msa1182800 AT1G61400.1 24.165 389 244 14 152 519 48 406 1.74e-11 67.4
Msa1182800 AT1G61400.2 24.165 389 244 14 152 519 48 406 1.78e-11 67.4
Msa1182800 AT1G61550.1 22.384 411 265 14 134 519 10 391 2.98e-11 67.0
Msa1182800 AT4G32300.1 26.689 296 174 14 151 429 47 316 4.81e-11 66.2
Msa1182800 AT5G24080.3 24.629 337 206 17 235 540 1 320 7.08e-11 65.5
Msa1182800 AT5G24080.1 24.629 337 206 17 235 540 1 320 7.08e-11 65.5

Find 117 sgRNAs with CRISPR-Local

Find 253 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCATGGTTGCTTTCACCTTC+TGG 0.162410 8_1:-17977195 Msa1182800:CDS
GTCAGGGTACTTGTATGTTT+TGG 0.212495 8_1:-17976638 Msa1182800:CDS
CTACACTGCTCTTCAGTAAA+AGG 0.260924 8_1:+17976184 None:intergenic
ATGGTGAACTTAACAGTCTT+TGG 0.262148 8_1:-17979142 Msa1182800:CDS
ACCAATCGCTATAGTGCTTT+TGG 0.264293 8_1:+17979423 None:intergenic
ACCAATGGCTATGGTACTTT+TGG 0.266017 8_1:+17977248 None:intergenic
AGTATAAGTACATATATTAA+AGG 0.280779 8_1:+17976453 None:intergenic
CCTTCTGGTGACTTTGCCTT+TGG 0.284286 8_1:-17977180 Msa1182800:CDS
TCAATCAGTGAGTAATGTTT+TGG 0.296727 8_1:+17976352 None:intergenic
CCTTCTCAAGTTGTGGATAA+AGG 0.297314 8_1:-17976853 Msa1182800:CDS
CACTGGCAACTTTGTGCTTC+AGG 0.298549 8_1:-17976932 Msa1182800:CDS
TCACTCTTAGTTGAACTCTT+AGG 0.318025 8_1:+17976502 None:intergenic
TCCATAACCTGACACCATTT+GGG 0.321032 8_1:+17977001 None:intergenic
AAAGCTTTCTTCAAGGCTTA+AGG 0.324891 8_1:-17976827 Msa1182800:CDS
CCCCAAATGGTGTCAGGTTA+TGG 0.333819 8_1:-17977002 Msa1182800:CDS
TTCCATAACCTGACACCATT+TGG 0.334304 8_1:+17977000 None:intergenic
TATAATAGCTTCTGCACTTT+GGG 0.334674 8_1:-17976403 Msa1182800:CDS
CAGCTGCCATATGGATCATT+AGG 0.345935 8_1:+17976328 None:intergenic
GCACATCCTTGTATGAAATC+TGG 0.361226 8_1:+17976301 None:intergenic
TTCCATAGCATGTCACCATT+TGG 0.368612 8_1:+17979233 None:intergenic
TGACAGGTATGGTGGAACTA+TGG 0.376095 8_1:+17976008 None:intergenic
CACAACTTGAGAAGGCAATA+AGG 0.378374 8_1:+17976861 None:intergenic
AAGAAGTTACCACTTTCAAA+TGG 0.384175 8_1:-17976094 Msa1182800:CDS
CATTCTATTAATTTACCATC+TGG 0.385953 8_1:-17976742 Msa1182800:CDS
TGACGCCTTCTTGCCTTCTC+AGG 0.401906 8_1:-17979099 Msa1182800:CDS
AAGGAAGAAAGCTTTCTTCA+AGG 0.414940 8_1:-17976834 Msa1182800:CDS
TGGTGAACTTAACAGTCTTT+GGG 0.415246 8_1:-17979141 Msa1182800:CDS
ATCAAGTGCTGGAGCTCAAT+TGG 0.420184 8_1:-17976671 Msa1182800:CDS
AGAGTTGATGCAACTCTTAA+TGG 0.425621 8_1:-17976070 Msa1182800:CDS
AGCTCCAGCACTTGATGTAC+TGG 0.427066 8_1:+17976678 None:intergenic
CTCCAAATGGTGACATGCTA+TGG 0.432332 8_1:-17979235 Msa1182800:CDS
TCAGGTTAGGTGATAGTTGT+TGG 0.437474 8_1:-17976120 Msa1182800:CDS
TCTGGCTTGCAGCTGCCATA+TGG 0.441887 8_1:+17976319 None:intergenic
ACTCTATCACCAAGCCCCTC+AGG 0.442069 8_1:+17979209 None:intergenic
CAATTGGTCTTTGACAAGTC+AGG 0.449002 8_1:-17976655 Msa1182800:CDS
GTGGAACTCACTGCCAATGA+TGG 0.454151 8_1:-17977039 Msa1182800:CDS
GAATCAGTGAGTAGAGGAGA+AGG 0.455286 8_1:+17979473 None:intergenic
AAAGAGACTAATTTCAGTAA+AGG 0.455417 8_1:-17979062 Msa1182800:CDS
CTTATTGCCTTCTCAAGTTG+TGG 0.458043 8_1:-17976860 Msa1182800:CDS
ACTGTTGTGTGGTATGCTAA+TGG 0.458658 8_1:-17977090 Msa1182800:CDS
TGCCTTCTCAGGTTGTTGAA+AGG 0.468344 8_1:-17979088 Msa1182800:CDS
GTGAAGGTTGGACCACAGTA+TGG 0.468347 8_1:-17976483 Msa1182800:CDS
CTATGGAACCCTGAGGGGCT+TGG 0.468655 8_1:-17979218 Msa1182800:CDS
ATCAGTGAGTAGAGGAGAAG+GGG 0.473970 8_1:+17979475 None:intergenic
CTGGTGCTAGCTTCTCCAAA+TGG 0.475850 8_1:-17979248 Msa1182800:intron
ATTGATCCTAATGATCCATA+TGG 0.477623 8_1:-17976334 Msa1182800:CDS
ATTTAATACTAATTTGTGAC+AGG 0.481714 8_1:+17975992 None:intergenic
AAGGAGACAGATTTCAGTAA+AGG 0.482797 8_1:-17976808 Msa1182800:CDS
AATCAGTGAGTAGAGGAGAA+GGG 0.485968 8_1:+17979474 None:intergenic
ATATAATAGCTTCTGCACTT+TGG 0.487312 8_1:-17976404 Msa1182800:CDS
GTATAAGTACATATATTAAA+GGG 0.488197 8_1:+17976454 None:intergenic
CCTTTATCCACAACTTGAGA+AGG 0.492964 8_1:+17976853 None:intergenic
AGAAGTTACCACTTTCAAAT+GGG 0.494879 8_1:-17976093 Msa1182800:CDS
AGTTAGCACCAACCCATCAT+TGG 0.498106 8_1:+17977026 None:intergenic
GAGGAGAAGGGGAAGTAGAG+AGG 0.502798 8_1:+17979486 None:intergenic
GCAGTGTAGGAAATCTTGTA+TGG 0.504417 8_1:-17976170 Msa1182800:CDS
ATACTAATTTGTGACAGGTA+TGG 0.507981 8_1:+17975997 None:intergenic
GGATGATTGTTTGTGTTCTG+TGG 0.510059 8_1:-17976149 Msa1182800:CDS
AAAAGAATCACCAATGGCTA+TGG 0.516781 8_1:+17977239 None:intergenic
AGTGCAGGTAGTGGTGTTTG+TGG 0.518931 8_1:-17976427 Msa1182800:CDS
TAACTTCCCCAAATGGTGTC+AGG 0.521731 8_1:-17977008 Msa1182800:CDS
TATCAGATTATGTGCTTCAA+AGG 0.523234 8_1:-17976207 Msa1182800:CDS
TGCAACTCTTAATGGTGCTA+AGG 0.523300 8_1:-17976062 Msa1182800:CDS
AGATGAAAACAACCATGAAA+AGG 0.524867 8_1:+17979375 None:intergenic
TTGGTGCTAACTTCCCCAAA+TGG 0.527112 8_1:-17977015 Msa1182800:CDS
TGTGGTGTGTTCAATGACAT+TGG 0.533191 8_1:-17979182 Msa1182800:CDS
AGCCTTTCAACAACCTGAGA+AGG 0.536142 8_1:+17979086 None:intergenic
TGAAGCACATAATCTGATAG+AGG 0.536988 8_1:+17976211 None:intergenic
CATTGGAAACTCTGTGCTTG+AGG 0.537589 8_1:-17979165 Msa1182800:CDS
AGACAGATTTCAGTAAAGGA+AGG 0.543432 8_1:-17976804 Msa1182800:CDS
GGTTTGAGCTACAACACTGA+TGG 0.545146 8_1:+17979444 None:intergenic
GAGATCCTTCTACAAAATGA+TGG 0.546683 8_1:-17979032 Msa1182800:intron
AAATCCAGTACATCAAGTGC+TGG 0.547246 8_1:-17976682 Msa1182800:CDS
GGAGACAGTCCTGCACCAAA+AGG 0.549672 8_1:-17977069 Msa1182800:CDS
AAACAACCATGAAAAGGAGT+TGG 0.550103 8_1:+17979381 None:intergenic
TGCAAGCCAGATTTCATACA+AGG 0.550125 8_1:-17976307 Msa1182800:CDS
GACAGGTATGGTGGAACTAT+GGG 0.550857 8_1:+17976009 None:intergenic
ACCAAAAGCACTATAGCGAT+TGG 0.553243 8_1:-17979424 Msa1182800:CDS
TCAACTCTCCCATTTGAAAG+TGG 0.563194 8_1:+17976085 None:intergenic
TGCACCAAAAGGATCAAAAG+TGG 0.563251 8_1:-17977058 Msa1182800:CDS
ACCAAAAGTACCATAGCCAT+TGG 0.565235 8_1:-17977249 Msa1182800:CDS
TATACTGTTAGTGCAGGTAG+TGG 0.565930 8_1:-17976436 Msa1182800:CDS
CTCTATCACCAAGCCCCTCA+GGG 0.566871 8_1:+17979210 None:intergenic
AAAAGATTAGTGTCTTTGAG+TGG 0.569176 8_1:+17977150 None:intergenic
AAGCAACCATGGAGATGTGC+TGG 0.569700 8_1:+17977206 None:intergenic
ATGGTAAATTCAACAGTCTG+TGG 0.569947 8_1:-17976909 Msa1182800:CDS
AATTGGTCTTTGACAAGTCA+GGG 0.572130 8_1:-17976654 Msa1182800:CDS
GGAAACTCTGTGCTTGAGGA+TGG 0.575358 8_1:-17979161 Msa1182800:CDS
GGCAACTTTGTGCTTCAGGA+TGG 0.577553 8_1:-17976928 Msa1182800:CDS
TGTACTTATACTGTTAGTGC+AGG 0.578443 8_1:-17976442 Msa1182800:CDS
CGCGGTGTATTGAATGACAC+TGG 0.581627 8_1:-17976949 Msa1182800:CDS
ATTGAAGAATCAGTGAGTAG+AGG 0.587377 8_1:+17979467 None:intergenic
CTAATTTGTGACAGGTATGG+TGG 0.587677 8_1:+17976000 None:intergenic
TGGTAAATTCAACAGTCTGT+GGG 0.591832 8_1:-17976908 Msa1182800:CDS
CTTGGTGATAGAGTTTCTTG+TGG 0.592795 8_1:-17979200 Msa1182800:CDS
AGACTAATTTCAGTAAAGGA+AGG 0.594962 8_1:-17979058 Msa1182800:CDS
CACAAGCCAGCACATCTCCA+TGG 0.597254 8_1:-17977212 Msa1182800:CDS
CCAGAAGGTGAAAGCAACCA+TGG 0.600409 8_1:+17977195 None:intergenic
GAAGGCAATAAGGTGTCACT+AGG 0.602021 8_1:+17976871 None:intergenic
GACATGCTATGGAACCCTGA+GGG 0.606652 8_1:-17979224 Msa1182800:CDS
ACTCACTGCCAATGATGGGT+TGG 0.610259 8_1:-17977034 Msa1182800:CDS
AAGAGTTCAACTAAGAGTGA+AGG 0.613595 8_1:-17976499 Msa1182800:CDS
AACAACCTGAGAAGGCAAGA+AGG 0.614766 8_1:+17979094 None:intergenic
GTTCAACTAAGAGTGAAGGT+TGG 0.619862 8_1:-17976495 Msa1182800:CDS
CTCAATGTTAAAGTTTCTCG+CGG 0.620103 8_1:-17976967 Msa1182800:CDS
TTTCCGAGAAAACTGTTGTG+TGG 0.627609 8_1:-17977101 Msa1182800:CDS
TGCAGTAAAAGAATCACCAA+TGG 0.629150 8_1:+17977233 None:intergenic
TGGAACTCACTGCCAATGAT+GGG 0.629211 8_1:-17977038 Msa1182800:CDS
GTCAGGTTATGGAATACTGA+AGG 0.629411 8_1:-17976991 Msa1182800:CDS
CCAAAGGCAAAGTCACCAGA+AGG 0.636933 8_1:+17977180 None:intergenic
CCATAACCTGACACCATTTG+GGG 0.639256 8_1:+17977002 None:intergenic
TTTGAGTGGAAGAAAACCAA+AGG 0.654690 8_1:+17977164 None:intergenic
AGGTATGGTGGAACTATGGG+AGG 0.656973 8_1:+17976012 None:intergenic
TGCTCTAAGATAAAATTGTG+TGG 0.657315 8_1:+17976564 None:intergenic
ACATGCTATGGAACCCTGAG+GGG 0.673116 8_1:-17979223 Msa1182800:CDS
AAAGGGCTTAGACCATACTG+TGG 0.757626 8_1:+17976471 None:intergenic
TGACATGCTATGGAACCCTG+AGG 0.767248 8_1:-17979225 Msa1182800:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTGTTAATTTAACATT+TGG - chr8_1:17976497-17976516 Msa1182800:CDS 10.0%
!! AAAAAATCTATAAAATCATA+TGG - chr8_1:17978048-17978067 Msa1182800:intron 10.0%
!! AAAAATAATAAACATATAGC+TGG - chr8_1:17977826-17977845 Msa1182800:intron 15.0%
!!! ACTTTTATTTTCTATGAAAA+AGG - chr8_1:17977917-17977936 Msa1182800:intron 15.0%
!!! CTTTTATTTTCTATGAAAAA+GGG - chr8_1:17977918-17977937 Msa1182800:intron 15.0%
!! GTATAAGTACATATATTAAA+GGG + chr8_1:17979042-17979061 None:intergenic 15.0%
!! AGTATAAGTACATATATTAA+AGG + chr8_1:17979043-17979062 None:intergenic 15.0%
!! ATATGATAATATCTGCAAAA+GGG + chr8_1:17977274-17977293 None:intergenic 20.0%
!! TATATGATAATATCTGCAAA+AGG + chr8_1:17977275-17977294 None:intergenic 20.0%
!!! TGATTATTGAATATGTTTTC+AGG + chr8_1:17977537-17977556 None:intergenic 20.0%
!! ATTAGATAAATATTCCTGTT+TGG - chr8_1:17977772-17977791 Msa1182800:intron 20.0%
!!! ATACATTACAATTTTGACAT+AGG + chr8_1:17977893-17977912 None:intergenic 20.0%
!!! ATCTTTTTTTGCTTTCAATT+TGG - chr8_1:17978359-17978378 Msa1182800:intron 20.0%
!!! ATATCATTTCTCTTTTTACT+TGG + chr8_1:17979228-17979247 None:intergenic 20.0%
! AAATGGAAAGCAAAAAAACA+TGG + chr8_1:17976184-17976203 None:intergenic 25.0%
!!! ATGTTTTTTTGCTTTCCATT+TGG - chr8_1:17976183-17976202 Msa1182800:CDS 25.0%
! AAAGAGACTAATTTCAGTAA+AGG - chr8_1:17976431-17976450 Msa1182800:CDS 25.0%
! TAACAGACAAAATTAGTGAA+CGG - chr8_1:17977366-17977385 Msa1182800:intron 25.0%
!! AATTACCTATTTTCTGTGAT+GGG + chr8_1:17977429-17977448 None:intergenic 25.0%
!! TAATTACCTATTTTCTGTGA+TGG + chr8_1:17977430-17977449 None:intergenic 25.0%
! ATCACAGAAAATAGGTAATT+AGG - chr8_1:17977429-17977448 Msa1182800:intron 25.0%
!!! TCTTAGGTTATAGTTTTACA+GGG + chr8_1:17977607-17977626 None:intergenic 25.0%
!!! ATCTTAGGTTATAGTTTTAC+AGG + chr8_1:17977608-17977627 None:intergenic 25.0%
!!! ATCGACTTTTTAACTATCTT+AGG + chr8_1:17977623-17977642 None:intergenic 25.0%
! ATTGATGTTAACATGAGTAT+TGG + chr8_1:17977711-17977730 None:intergenic 25.0%
!!! TGTTTGCTATAAAAAGCATA+TGG - chr8_1:17978127-17978146 Msa1182800:intron 25.0%
! AGAAAGACATGTTTATAATG+TGG + chr8_1:17978165-17978184 None:intergenic 25.0%
! CATTCTATTAATTTACCATC+TGG - chr8_1:17978751-17978770 Msa1182800:intron 25.0%
!! TTTTCATTAACATAACCAGA+TGG + chr8_1:17978769-17978788 None:intergenic 25.0%
AGATGAAAACAACCATGAAA+AGG + chr8_1:17976121-17976140 None:intergenic 30.0%
! TCTTCTAGTAACTTTTCGTT+AGG - chr8_1:17976138-17976157 Msa1182800:CDS 30.0%
AGAAATGTTTGCATACCAAA+TGG + chr8_1:17976201-17976220 None:intergenic 30.0%
AGACTAATTTCAGTAAAGGA+AGG - chr8_1:17976435-17976454 Msa1182800:CDS 30.0%
! GATTACCATCATTTTGTAGA+AGG + chr8_1:17976469-17976488 None:intergenic 30.0%
! ACTATGAAAGTGTAAAACTG+TGG - chr8_1:17976543-17976562 Msa1182800:CDS 30.0%
TTGAATGTAATAACATGTGC+CGG + chr8_1:17976695-17976714 None:intergenic 30.0%
! AGAGCAACTCTTAATTTTGA+CGG - chr8_1:17976865-17976884 Msa1182800:CDS 30.0%
!!! CAACTAGTCTGTTATTTTCT+CGG - chr8_1:17976986-17977005 Msa1182800:CDS 30.0%
AAAGTCGATAACAAAAAGAG+AGG - chr8_1:17977634-17977653 Msa1182800:intron 30.0%
AAGAGAGGAATTTGTAGATA+TGG - chr8_1:17977649-17977668 Msa1182800:intron 30.0%
AATTTGTAGATATGGTTACC+TGG - chr8_1:17977657-17977676 Msa1182800:intron 30.0%
AATTTACTGATACCAAATCC+AGG + chr8_1:17977678-17977697 None:intergenic 30.0%
TACGAATTAAAGTTCCAAAC+AGG + chr8_1:17977789-17977808 None:intergenic 30.0%
CTAAAAATCAACACACAAGT+TGG + chr8_1:17977979-17977998 None:intergenic 30.0%
! ACCAAGTGCTTTAAATTTGA+TGG - chr8_1:17978017-17978036 Msa1182800:intron 30.0%
! TCCATCAAATTTAAAGCACT+TGG + chr8_1:17978021-17978040 None:intergenic 30.0%
AAAAGATTAGTGTCTTTGAG+TGG + chr8_1:17978346-17978365 None:intergenic 30.0%
! ACAGTTTTCTCGGAAATTTT+GGG + chr8_1:17978388-17978407 None:intergenic 30.0%
! AACAGTTTTCTCGGAAATTT+TGG + chr8_1:17978389-17978408 None:intergenic 30.0%
!! TGCTCTAAGATAAAATTGTG+TGG + chr8_1:17978932-17978951 None:intergenic 30.0%
! AGAGCAACTCTTAATTTTGA+TGG - chr8_1:17976865-17976884 Msa1182800:CDS 30.0%
ATATAATAGCTTCTGCACTT+TGG - chr8_1:17979089-17979108 Msa1182800:CDS 30.0%
TATAATAGCTTCTGCACTTT+GGG - chr8_1:17979090-17979109 Msa1182800:CDS 30.0%
! TCAATCAGTGAGTAATGTTT+TGG + chr8_1:17979144-17979163 None:intergenic 30.0%
ATTGATCCTAATGATCCATA+TGG - chr8_1:17979159-17979178 Msa1182800:CDS 30.0%
TATCAGATTATGTGCTTCAA+AGG - chr8_1:17979286-17979305 Msa1182800:intron 30.0%
AAGAAGTTACCACTTTCAAA+TGG - chr8_1:17979399-17979418 Msa1182800:CDS 30.0%
AGAAGTTACCACTTTCAAAT+GGG - chr8_1:17979400-17979419 Msa1182800:CDS 30.0%
ATTGAAGAATCAGTGAGTAG+AGG + chr8_1:17976029-17976048 None:intergenic 35.0%
AAACAACCATGAAAAGGAGT+TGG + chr8_1:17976115-17976134 None:intergenic 35.0%
ATGGTGAACTTAACAGTCTT+TGG - chr8_1:17976351-17976370 Msa1182800:CDS 35.0%
TGGTGAACTTAACAGTCTTT+GGG - chr8_1:17976352-17976371 Msa1182800:CDS 35.0%
GAGATCCTTCTACAAAATGA+TGG - chr8_1:17976461-17976480 Msa1182800:CDS 35.0%
!! CAATTAGTCTTTGACAGATC+AGG - chr8_1:17976582-17976601 Msa1182800:CDS 35.0%
ATCAGGTTACTTGTATGTCT+TGG - chr8_1:17976599-17976618 Msa1182800:CDS 35.0%
TCAGGTTACTTGTATGTCTT+GGG - chr8_1:17976600-17976619 Msa1182800:CDS 35.0%
TATTATCTGTGTCAACGTGT+TGG - chr8_1:17976734-17976753 Msa1182800:CDS 35.0%
!! GAGCAACTCTTAATTTTGAC+GGG - chr8_1:17976866-17976885 Msa1182800:CDS 35.0%
!! AGCAACTCTTAATTTTGACG+GGG - chr8_1:17976867-17976886 Msa1182800:CDS 35.0%
TGCAAGTTGTTCCAAATTCA+TGG - chr8_1:17977015-17977034 Msa1182800:CDS 35.0%
AGTTGTTCCAAATTCATGGA+CGG - chr8_1:17977019-17977038 Msa1182800:CDS 35.0%
! TGGCGATTCTAGTGATTTTA+AGG + chr8_1:17977316-17977335 None:intergenic 35.0%
AACATTGAGATGATTCGATG+TGG + chr8_1:17977336-17977355 None:intergenic 35.0%
TCACATTACTAGAGTAACAG+TGG + chr8_1:17977568-17977587 None:intergenic 35.0%
!! TAGTTTTACAGGGTTGAAGT+TGG + chr8_1:17977597-17977616 None:intergenic 35.0%
TAGATATGGTTACCTGGATT+TGG - chr8_1:17977663-17977682 Msa1182800:intron 35.0%
!! CTTCTTAAGTGGACTTTTCA+GGG + chr8_1:17977861-17977880 None:intergenic 35.0%
!! TCTTCTTAAGTGGACTTTTC+AGG + chr8_1:17977862-17977881 None:intergenic 35.0%
!! TGAGCACTAGTCTTTCAATT+TGG + chr8_1:17978075-17978094 None:intergenic 35.0%
!! GCTCAGTGCTTTAATTTTGA+TGG - chr8_1:17978090-17978109 Msa1182800:intron 35.0%
ATCAGTAAGTAGAGGAGAAA+GGG + chr8_1:17978196-17978215 None:intergenic 35.0%
AATCAGTAAGTAGAGGAGAA+AGG + chr8_1:17978197-17978216 None:intergenic 35.0%
ATTGCAGAATCAGTAAGTAG+AGG + chr8_1:17978204-17978223 None:intergenic 35.0%
AAAAGAATCACCAATGGCTA+TGG + chr8_1:17978257-17978276 None:intergenic 35.0%
TGCAGTAAAAGAATCACCAA+TGG + chr8_1:17976088-17976107 None:intergenic 35.0%
TTTGAGTGGAAGAAAACCAA+AGG + chr8_1:17978332-17978351 None:intergenic 35.0%
! ATACCACACAACAGTTTTCT+CGG + chr8_1:17978398-17978417 None:intergenic 35.0%
!! AGTTCCACTTTTGATCCTTT+TGG + chr8_1:17978442-17978461 None:intergenic 35.0%
CTCAATGTTAAAGTTTCTCG+CGG - chr8_1:17978526-17978545 Msa1182800:intron 35.0%
ATGGTAAATTCAACAGTCTG+TGG - chr8_1:17978584-17978603 Msa1182800:intron 35.0%
TGGTAAATTCAACAGTCTGT+GGG - chr8_1:17978585-17978604 Msa1182800:intron 35.0%
AAGGAAGAAAGCTTTCTTCA+AGG - chr8_1:17978659-17978678 Msa1182800:intron 35.0%
AAAGCTTTCTTCAAGGCTTA+AGG - chr8_1:17978666-17978685 Msa1182800:intron 35.0%
AAGGAGACAGATTTCAGTAA+AGG - chr8_1:17978685-17978704 Msa1182800:intron 35.0%
AGACAGATTTCAGTAAAGGA+AGG - chr8_1:17978689-17978708 Msa1182800:intron 35.0%
! GAACTCGTTTTACAAAGTGA+TGG - chr8_1:17978715-17978734 Msa1182800:intron 35.0%
!! AATTGGTCTTTGACAAGTCA+GGG - chr8_1:17978839-17978858 Msa1182800:intron 35.0%
!! TCAGGGTACTTGTATGTTTT+GGG - chr8_1:17978856-17978875 Msa1182800:intron 35.0%
TCACTCTTAGTTGAACTCTT+AGG + chr8_1:17978994-17979013 None:intergenic 35.0%
AAGAGTTCAACTAAGAGTGA+AGG - chr8_1:17978994-17979013 Msa1182800:intron 35.0%
TGTACTTATACTGTTAGTGC+AGG - chr8_1:17979051-17979070 Msa1182800:CDS 35.0%
!! TGTTTTGGACACTGACATTT+TGG + chr8_1:17979129-17979148 None:intergenic 35.0%
!! AGATTTTGATCGACACTGAT+TGG - chr8_1:17979259-17979278 Msa1182800:intron 35.0%
TGAAGCACATAATCTGATAG+AGG + chr8_1:17979285-17979304 None:intergenic 35.0%
!! AGAGTTGATGCAACTCTTAA+TGG - chr8_1:17979423-17979442 Msa1182800:CDS 35.0%
AATCAGTGAGTAGAGGAGAA+GGG + chr8_1:17976022-17976041 None:intergenic 40.0%
!! ACCAAAAGCACTATAGCGAT+TGG - chr8_1:17976069-17976088 Msa1182800:CDS 40.0%
! ACCAATCGCTATAGTGCTTT+TGG + chr8_1:17976073-17976092 None:intergenic 40.0%
! CACAAACCAACTCCTTTTCA+TGG - chr8_1:17976106-17976125 Msa1182800:CDS 40.0%
AGAAACTCACTGCCAATGAT+GGG - chr8_1:17976222-17976241 Msa1182800:CDS 40.0%
TTCCATAGCATGTCACCATT+TGG + chr8_1:17976263-17976282 None:intergenic 40.0%
! CTTGGTGATAGAGTTTCTTG+TGG - chr8_1:17976293-17976312 Msa1182800:CDS 40.0%
TGTGGTGTGTTCAATGACAT+TGG - chr8_1:17976311-17976330 Msa1182800:CDS 40.0%
TAAAACTGTGGATCCAGTTC+TGG - chr8_1:17976555-17976574 Msa1182800:CDS 40.0%
GACTAATTGAGTTCCAGAAC+TGG + chr8_1:17976571-17976590 None:intergenic 40.0%
ATGCCTTAGATCATAGGTGT+CGG + chr8_1:17976636-17976655 None:intergenic 40.0%
TCACTCTCAGTTGAACTCTT+TGG + chr8_1:17976913-17976932 None:intergenic 40.0%
AAGAGTTCAACTGAGAGTGA+AGG - chr8_1:17976913-17976932 Msa1182800:CDS 40.0%
! ACTTTTGCCGTCCATGAATT+TGG + chr8_1:17977029-17977048 None:intergenic 40.0%
CATGGACGGCAAAAGTAAAT+AGG - chr8_1:17977033-17977052 Msa1182800:CDS 40.0%
AGTAAATAGGTGGAAACCGA+AGG - chr8_1:17977046-17977065 Msa1182800:CDS 40.0%
GAAACCGAAGGAAATTCAGA+CGG - chr8_1:17977058-17977077 Msa1182800:CDS 40.0%
TACTCCGTCTGAATTTCCTT+CGG + chr8_1:17977065-17977084 None:intergenic 40.0%
ACATGACATGACAGAAGAAG+AGG - chr8_1:17977119-17977138 Msa1182800:CDS 40.0%
CAAAGAAGGTTGGCATCATT+GGG - chr8_1:17977187-17977206 Msa1182800:CDS 40.0%
CAGACAAAATTAGTGAACGG+TGG - chr8_1:17977369-17977388 Msa1182800:intron 40.0%
GCTATGAATGTAGTGCTTCA+TGG + chr8_1:17977455-17977474 None:intergenic 40.0%
GGACTTGTGTTCTTCTTAAG+TGG + chr8_1:17977872-17977891 None:intergenic 40.0%
! CACACAAGTTGGCTTAACTT+TGG + chr8_1:17977968-17977987 None:intergenic 40.0%
!! ACCAAAAGTACCATAGCCAT+TGG - chr8_1:17978244-17978263 Msa1182800:intron 40.0%
! ACCAATGGCTATGGTACTTT+TGG + chr8_1:17978248-17978267 None:intergenic 40.0%
TTTCCGAGAAAACTGTTGTG+TGG - chr8_1:17978392-17978411 Msa1182800:intron 40.0%
ACTGTTGTGTGGTATGCTAA+TGG - chr8_1:17978403-17978422 Msa1182800:intron 40.0%
!! ACTTTTGATCCTTTTGGTGC+AGG + chr8_1:17978436-17978455 None:intergenic 40.0%
TGCACCAAAAGGATCAAAAG+TGG - chr8_1:17978435-17978454 Msa1182800:intron 40.0%
TCCATAACCTGACACCATTT+GGG + chr8_1:17978495-17978514 None:intergenic 40.0%
TTCCATAACCTGACACCATT+TGG + chr8_1:17978496-17978515 None:intergenic 40.0%
GTCAGGTTATGGAATACTGA+AGG - chr8_1:17978502-17978521 Msa1182800:intron 40.0%
CACAACTTGAGAAGGCAATA+AGG + chr8_1:17978635-17978654 None:intergenic 40.0%
! CTTATTGCCTTCTCAAGTTG+TGG - chr8_1:17978633-17978652 Msa1182800:intron 40.0%
CCTTTATCCACAACTTGAGA+AGG + chr8_1:17978643-17978662 None:intergenic 40.0%
! CCTTCTCAAGTTGTGGATAA+AGG - chr8_1:17978640-17978659 Msa1182800:intron 40.0%
AAATCCAGTACATCAAGTGC+TGG - chr8_1:17978811-17978830 Msa1182800:intron 40.0%
!! CAATTGGTCTTTGACAAGTC+AGG - chr8_1:17978838-17978857 Msa1182800:intron 40.0%
!! GTCAGGGTACTTGTATGTTT+TGG - chr8_1:17978855-17978874 Msa1182800:intron 40.0%
GTTCAACTAAGAGTGAAGGT+TGG - chr8_1:17978998-17979017 Msa1182800:intron 40.0%
TATACTGTTAGTGCAGGTAG+TGG - chr8_1:17979057-17979076 Msa1182800:CDS 40.0%
TGCAAGCCAGATTTCATACA+AGG - chr8_1:17979186-17979205 Msa1182800:CDS 40.0%
GCACATCCTTGTATGAAATC+TGG + chr8_1:17979195-17979214 None:intergenic 40.0%
CTACACTGCTCTTCAGTAAA+AGG + chr8_1:17979312-17979331 None:intergenic 40.0%
! CTTTTACTGAAGAGCAGTGT+AGG - chr8_1:17979310-17979329 Msa1182800:intron 40.0%
GCAGTGTAGGAAATCTTGTA+TGG - chr8_1:17979323-17979342 Msa1182800:intron 40.0%
GGATGATTGTTTGTGTTCTG+TGG - chr8_1:17979344-17979363 Msa1182800:intron 40.0%
!! TGTGTTCTGTGGCTATTTTC+AGG - chr8_1:17979355-17979374 Msa1182800:intron 40.0%
!!! TCTGTGGCTATTTTCAGGTT+AGG - chr8_1:17979360-17979379 Msa1182800:CDS 40.0%
TCAGGTTAGGTGATAGTTGT+TGG - chr8_1:17979373-17979392 Msa1182800:CDS 40.0%
TCAACTCTCCCATTTGAAAG+TGG + chr8_1:17979411-17979430 None:intergenic 40.0%
! TGCAACTCTTAATGGTGCTA+AGG - chr8_1:17979431-17979450 Msa1182800:CDS 40.0%
! CTAATTTGTGACAGGTATGG+TGG + chr8_1:17979496-17979515 None:intergenic 40.0%
ATCAGTGAGTAGAGGAGAAG+GGG + chr8_1:17976021-17976040 None:intergenic 45.0%
GAATCAGTGAGTAGAGGAGA+AGG + chr8_1:17976023-17976042 None:intergenic 45.0%
GGTTTGAGCTACAACACTGA+TGG + chr8_1:17976052-17976071 None:intergenic 45.0%
GAGAAACTCACTGCCAATGA+TGG - chr8_1:17976221-17976240 Msa1182800:CDS 45.0%
CTCCAAATGGTGACATGCTA+TGG - chr8_1:17976258-17976277 Msa1182800:CDS 45.0%
CATTGGAAACTCTGTGCTTG+AGG - chr8_1:17976328-17976347 Msa1182800:CDS 45.0%
AACAACCTGAGAAGGCAAGA+AGG + chr8_1:17976402-17976421 None:intergenic 45.0%
! TGCCTTCTCAGGTTGTTGAA+AGG - chr8_1:17976405-17976424 Msa1182800:CDS 45.0%
AGCCTTTCAACAACCTGAGA+AGG + chr8_1:17976410-17976429 None:intergenic 45.0%
! ACCTATGATCTAAGGCATGC+CGG - chr8_1:17976638-17976657 Msa1182800:CDS 45.0%
!! ATGAAGCACCGACATAGACA+CGG + chr8_1:17976768-17976787 None:intergenic 45.0%
! TGTCTATGTCGGTGCTTCAT+AGG - chr8_1:17976768-17976787 Msa1182800:CDS 45.0%
GTTCAACTGAGAGTGAAGGT+TGG - chr8_1:17976917-17976936 Msa1182800:CDS 45.0%
GGACGGCAAAAGTAAATAGG+TGG - chr8_1:17977036-17977055 Msa1182800:CDS 45.0%
ACATGACAGAAGAAGAGGTG+AGG - chr8_1:17977124-17977143 Msa1182800:CDS 45.0%
! GAAGAGGTGAGGATGGTTTT+AGG - chr8_1:17977135-17977154 Msa1182800:CDS 45.0%
!!! GGATGGTTTTAGGTATGCAG+TGG - chr8_1:17977145-17977164 Msa1182800:CDS 45.0%
GCAAAGAAGGTTGGCATCAT+TGG - chr8_1:17977186-17977205 Msa1182800:CDS 45.0%
TGGCATCATTGGGACGAAAA+TGG - chr8_1:17977197-17977216 Msa1182800:CDS 45.0%
GGCATCATTGGGACGAAAAT+GGG - chr8_1:17977198-17977217 Msa1182800:CDS 45.0%
ATCATTGGGACGAAAATGGG+CGG - chr8_1:17977201-17977220 Msa1182800:CDS 45.0%
CGAAAACTCTAAGTTTGCGC+GGG + chr8_1:17977404-17977423 None:intergenic 45.0%
GCGAAAACTCTAAGTTTGCG+CGG + chr8_1:17977405-17977424 None:intergenic 45.0%
TCGCACCCATCACAGAAAAT+AGG - chr8_1:17977421-17977440 Msa1182800:intron 45.0%
TGGAACTCACTGCCAATGAT+GGG - chr8_1:17978455-17978474 Msa1182800:intron 45.0%
AGTTAGCACCAACCCATCAT+TGG + chr8_1:17978470-17978489 None:intergenic 45.0%
!! TTGGTGCTAACTTCCCCAAA+TGG - chr8_1:17978478-17978497 Msa1182800:intron 45.0%
TAACTTCCCCAAATGGTGTC+AGG - chr8_1:17978485-17978504 Msa1182800:intron 45.0%
CCATAACCTGACACCATTTG+GGG + chr8_1:17978494-17978513 None:intergenic 45.0%
!! GAAGGCAATAAGGTGTCACT+AGG + chr8_1:17978625-17978644 None:intergenic 45.0%
!! ATCAAGTGCTGGAGCTCAAT+TGG - chr8_1:17978822-17978841 Msa1182800:intron 45.0%
AAAGGGCTTAGACCATACTG+TGG + chr8_1:17979025-17979044 None:intergenic 45.0%
CAGCTGCCATATGGATCATT+AGG + chr8_1:17979168-17979187 None:intergenic 45.0%
! GACAGGTATGGTGGAACTAT+GGG + chr8_1:17979487-17979506 None:intergenic 45.0%
! TGACAGGTATGGTGGAACTA+TGG + chr8_1:17979488-17979507 None:intergenic 45.0%
! CTGGTGCTAGCTTCTCCAAA+TGG - chr8_1:17976245-17976264 Msa1182800:CDS 50.0%
TGACATGCTATGGAACCCTG+AGG - chr8_1:17976268-17976287 Msa1182800:CDS 50.0%
GACATGCTATGGAACCCTGA+GGG - chr8_1:17976269-17976288 Msa1182800:CDS 50.0%
ACATGCTATGGAACCCTGAG+GGG - chr8_1:17976270-17976289 Msa1182800:CDS 50.0%
GGAAACTCTGTGCTTGAGGA+TGG - chr8_1:17976332-17976351 Msa1182800:CDS 50.0%
! GCACCGACACCTATGATCTA+AGG - chr8_1:17976630-17976649 Msa1182800:CDS 50.0%
ACCGGCATGCCTTAGATCAT+AGG + chr8_1:17976642-17976661 None:intergenic 50.0%
! TCTGTGTCAACGTGTTGGTG+CGG - chr8_1:17976739-17976758 Msa1182800:CDS 50.0%
! TGTCGGTGCTTCATAGGTCT+TGG - chr8_1:17976774-17976793 Msa1182800:CDS 50.0%
! GTCGGTGCTTCATAGGTCTT+GGG - chr8_1:17976775-17976794 Msa1182800:CDS 50.0%
GACAGAAGAAGAGGTGAGGA+TGG - chr8_1:17977128-17977147 Msa1182800:CDS 50.0%
!!! GGTTTTAGGTATGCAGTGGC+CGG - chr8_1:17977149-17977168 Msa1182800:CDS 50.0%
ACTCTAAGTTTGCGCGGGAA+TGG + chr8_1:17977399-17977418 None:intergenic 50.0%
GGTTTGAGCTACAGCACAGA+TGG + chr8_1:17978227-17978246 None:intergenic 50.0%
AAGCAACCATGGAGATGTGC+TGG + chr8_1:17978290-17978309 None:intergenic 50.0%
CCAGAAGGTGAAAGCAACCA+TGG + chr8_1:17978301-17978320 None:intergenic 50.0%
! CCATGGTTGCTTTCACCTTC+TGG - chr8_1:17978298-17978317 Msa1182800:intron 50.0%
CCAAAGGCAAAGTCACCAGA+AGG + chr8_1:17978316-17978335 None:intergenic 50.0%
CCTTCTGGTGACTTTGCCTT+TGG - chr8_1:17978313-17978332 Msa1182800:intron 50.0%
GTGGAACTCACTGCCAATGA+TGG - chr8_1:17978454-17978473 Msa1182800:intron 50.0%
!! ACTCACTGCCAATGATGGGT+TGG - chr8_1:17978459-17978478 Msa1182800:intron 50.0%
! CCCCAAATGGTGTCAGGTTA+TGG - chr8_1:17978491-17978510 Msa1182800:intron 50.0%
CGCGGTGTATTGAATGACAC+TGG - chr8_1:17978544-17978563 Msa1182800:intron 50.0%
CACTGGCAACTTTGTGCTTC+AGG - chr8_1:17978561-17978580 Msa1182800:intron 50.0%
GGCAACTTTGTGCTTCAGGA+TGG - chr8_1:17978565-17978584 Msa1182800:intron 50.0%
AGCTCCAGCACTTGATGTAC+TGG + chr8_1:17978818-17978837 None:intergenic 50.0%
GTGAAGGTTGGACCACAGTA+TGG - chr8_1:17979010-17979029 Msa1182800:intron 50.0%
! AGTGCAGGTAGTGGTGTTTG+TGG - chr8_1:17979066-17979085 Msa1182800:CDS 50.0%
! AGGTATGGTGGAACTATGGG+AGG + chr8_1:17979484-17979503 None:intergenic 50.0%
GAGGAGAAGGGGAAGTAGAG+AGG + chr8_1:17976010-17976029 None:intergenic 55.0%
!! ACTCACTGCCAATGATGGGC+TGG - chr8_1:17976226-17976245 Msa1182800:CDS 55.0%
AGCTAGCACCAGCCCATCAT+TGG + chr8_1:17976237-17976256 None:intergenic 55.0%
CTCTATCACCAAGCCCCTCA+GGG + chr8_1:17976286-17976305 None:intergenic 55.0%
ACTCTATCACCAAGCCCCTC+AGG + chr8_1:17976287-17976306 None:intergenic 55.0%
TGACGCCTTCTTGCCTTCTC+AGG - chr8_1:17976394-17976413 Msa1182800:CDS 55.0%
!! AACATGTGCCGGTGTCGTGT+CGG + chr8_1:17976684-17976703 None:intergenic 55.0%
TGCGGTGTCCGTGTCTATGT+CGG - chr8_1:17976757-17976776 Msa1182800:CDS 55.0%
AGAGACTCACCCTGTCGTCT+TGG + chr8_1:17977244-17977263 None:intergenic 55.0%
AAGACGACAGGGTGAGTCTC+TGG - chr8_1:17977243-17977262 Msa1182800:CDS 55.0%
CACAAGCCAGCACATCTCCA+TGG - chr8_1:17978281-17978300 Msa1182800:intron 55.0%
GGAGACAGTCCTGCACCAAA+AGG - chr8_1:17978424-17978443 Msa1182800:intron 55.0%
! TCTGGCTTGCAGCTGCCATA+TGG + chr8_1:17979177-17979196 None:intergenic 55.0%
CTATGGAACCCTGAGGGGCT+TGG - chr8_1:17976275-17976294 Msa1182800:CDS 60.0%
GACTGACACGTGTCGGACAC+CGG + chr8_1:17976660-17976679 None:intergenic 60.0%
TGTCGGGGACTGACACGTGT+CGG + chr8_1:17976667-17976686 None:intergenic 60.0%
!! ACATGTGCCGGTGTCGTGTC+GGG + chr8_1:17976683-17976702 None:intergenic 60.0%
ACCTTCTTTGCCACGCCGTC+CGG + chr8_1:17977177-17977196 None:intergenic 60.0%
GACGGCGTGGCAAAGAAGGT+TGG - chr8_1:17977177-17977196 Msa1182800:CDS 60.0%
CGAAAATGGGCGGAAGCTGC+TGG - chr8_1:17977211-17977230 Msa1182800:CDS 60.0%
TGGAGTGGCTCCAAGACGAC+AGG - chr8_1:17977231-17977250 Msa1182800:CDS 60.0%
GGAGTGGCTCCAAGACGACA+GGG - chr8_1:17977232-17977251 Msa1182800:CDS 60.0%
GTCAGTCCCCGACACGACAC+CGG - chr8_1:17976673-17976692 Msa1182800:CDS 65.0%
!! CATGTGCCGGTGTCGTGTCG+GGG + chr8_1:17976682-17976701 None:intergenic 65.0%
ATGGGCGGAAGCTGCTGGAG+TGG - chr8_1:17977216-17977235 Msa1182800:CDS 65.0%
AGGTATGCAGTGGCCGGCGC+CGG - chr8_1:17977155-17977174 Msa1182800:CDS 70.0%
GCCGGACGGCGTGGCAAAGA+AGG - chr8_1:17977173-17977192 Msa1182800:CDS 70.0%
! ATGCAGTGGCCGGCGCCGGA+CGG - chr8_1:17977159-17977178 Msa1182800:CDS 75.0%
TTTGCCACGCCGTCCGGCGC+CGG + chr8_1:17977171-17977190 None:intergenic 75.0%
!! GTGGCCGGCGCCGGACGGCG+TGG - chr8_1:17977164-17977183 Msa1182800:CDS 90.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 17976000 17979515 17976000 ID=Msa1182800;Name=Msa1182800
chr8_1 mRNA 17976000 17979515 17976000 ID=Msa1182800-mRNA-1;Parent=Msa1182800;Name=Msa1182800-mRNA-1;_AED=0.17;_eAED=0.17;_QI=0|0|0|1|1|1|3|0|554
chr8_1 exon 17979356 17979515 17979356 ID=Msa1182800-mRNA-1:exon:6788;Parent=Msa1182800-mRNA-1
chr8_1 exon 17979033 17979260 17979033 ID=Msa1182800-mRNA-1:exon:6787;Parent=Msa1182800-mRNA-1
chr8_1 exon 17976000 17977276 17976000 ID=Msa1182800-mRNA-1:exon:6786;Parent=Msa1182800-mRNA-1
chr8_1 CDS 17979356 17979515 17979356 ID=Msa1182800-mRNA-1:cds;Parent=Msa1182800-mRNA-1
chr8_1 CDS 17979033 17979260 17979033 ID=Msa1182800-mRNA-1:cds;Parent=Msa1182800-mRNA-1
chr8_1 CDS 17976000 17977276 17976000 ID=Msa1182800-mRNA-1:cds;Parent=Msa1182800-mRNA-1
Gene Sequence

>Msa1182800

ATGGCTTCCTCTCTACTTCCCCTTCTCCTCTACTCACTGATTCTTCAATCCATCAGTGTTGTAGCTCAAACCAAAAGCACTATAGCGATTGGTGATTCTTTTACTGCACAAACCAACTCCTTTTCATGGTTGTTTTCATCTTCTAGTAACTTTTCGTTAGCTTCTCCAAATGGTGACATGCTATGGAACCCTGAGGGGCTTGGTGATAGAGTTTCTTGTGGTGTGTTCAATGACATTGGAAACTCTGTGCTTGAGGATGGTGAACTTAACAGTCTTTGGGAGACTTTCAAGTTTTCTAGTGACGCCTTCTTGCCTTCTCAGGTTGTTGAAAGGCTTAAAGAGACTAATTTCAGTAAAGGAAGGTGTGAGATCCTTCTACAAAATGATGCTCAAACCAAAAGTACCATAGCCATTGGTGATTCTTTTACTGCACAAGCCAGCACATCTCCATGGTTGCTTTCACCTTCTGGTGACTTTGCCTTTGGTTTTCTTCCACTCAAAGACACTAATCTTTTTTTGCTTTCAATTTGGTATCCCAAAATTTCCGAGAAAACTGTTGTGTGGTATGCTAATGGAGACAGTCCTGCACCAAAAGGATCAAAAGTGGAACTCACTGCCAATGATGGGTTGGTGCTAACTTCCCCAAATGGTGTCAGGTTATGGAATACTGAAGGACTCAATGTTAAAGTTTCTCGCGGTGTATTGAATGACACTGGCAACTTTGTGCTTCAGGATGGTAAATTCAACAGTCTGTGGGAGACTTTCAAGTTTCCTAGTGACACCTTATTGCCTTCTCAAGTTGTGGATAAAGGAAGAAAGCTTTCTTCAAGGCTTAAGGAGACAGATTTCAGTAAAGGAAGGTTTGAACTCGTTTTACAAAGTGATGGTAATCTTGTTATGCATTCTATTAATTTACCATCTGGTTATGTTAATGAAAACTACTTTGAAAGTAATACTGTTAAATCCAGTACATCAAGTGCTGGAGCTCAATTGGTCTTTGACAAGTCAGGGTACTTGTATGTTTTGGGAGAGAACAATGAGAAATATAATGTGTTTGAAGAAGAGAGTAACGTCTCGACCACACAATTTTATCTTAGAGCAACTCTTAATTTTGATGGAGTGTTCACACTTTACAAGCATCCTAAGAGTTCAACTAAGAGTGAAGGTTGGACCACAGTATGGTCTAAGCCCTTTAATATATGTACTTATACTGTTAGTGCAGGTAGTGGTGTTTGTGGATATAATAGCTTCTGCACTTTGGGAGTTGATAAGAGACCAAAATGTCAGTGTCCAAAACATTACTCACTGATTGATCCTAATGATCCATATGGCAGCTGCAAGCCAGATTTCATACAAGGATGTGCAGAAGATGATCCAAGTAAAAAGAGAAATGATATCTATGAGTTCGAGATTTTGATCGACACTGATTGGCCTCTATCAGATTATGTGCTTCAAAGGCCTTTTACTGAAGAGCAGTGTAGGAAATCTTGTATGGATGATTGTTTGTGTTCTGTGGCTATTTTCAGGTTAGGTGATAGTTGTTGGAAGAAGAAGTTACCACTTTCAAATGGGAGAGTTGATGCAACTCTTAATGGTGCTAAGGCTTTCTTGAAAGTGAGAAAAGATAATGCCTCCCATAGTTCCACCATACCTGTCACAAATTAG
Protein sequence

>Msa1182800

MASSLLPLLLYSLILQSISVVAQTKSTIAIGDSFTAQTNSFSWLFSSSSNFSLASPNGDMLWNPEGLGDRVSCGVFNDIGNSVLEDGELNSLWETFKFSSDAFLPSQVVERLKETNFSKGRCEILLQNDAQTKSTIAIGDSFTAQASTSPWLLSPSGDFAFGFLPLKDTNLFLLSIWYPKISEKTVVWYANGDSPAPKGSKVELTANDGLVLTSPNGVRLWNTEGLNVKVSRGVLNDTGNFVLQDGKFNSLWETFKFPSDTLLPSQVVDKGRKLSSRLKETDFSKGRFELVLQSDGNLVMHSINLPSGYVNENYFESNTVKSSTSSAGAQLVFDKSGYLYVLGENNEKYNVFEEESNVSTTQFYLRATLNFDGVFTLYKHPKSSTKSEGWTTVWSKPFNICTYTVSAGSGVCGYNSFCTLGVDKRPKCQCPKHYSLIDPNDPYGSCKPDFIQGCAEDDPSKKRNDIYEFEILIDTDWPLSDYVLQRPFTEEQCRKSCMDDCLCSVAIFRLGDSCWKKKLPLSNGRVDATLNGAKAFLKVRKDNASHSSTIPVTN*