AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0675010


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0675010 MtrunA17_Chr4g0018241 94.595 111 6 0 1 111 87 197 8.45e-74 217
Msa0675010 MtrunA17_Chr4g0018251 91.892 111 9 0 1 111 87 197 9.27e-72 212
Msa0675010 MtrunA17_Chr4g0018281 82.727 110 19 0 1 110 87 196 3.47e-62 187
Msa0675010 MtrunA17_Chr5g0398871 46.364 110 56 1 4 110 102 211 3.05e-30 107
Msa0675010 MtrunA17_Chr3g0079401 46.667 105 54 1 8 110 83 187 4.54e-29 103
Msa0675010 MtrunA17_Chr3g0079381 45.794 107 56 1 6 110 80 186 5.80e-29 103
Msa0675010 MtrunA17_Chr4g0056601 43.363 113 61 1 1 110 90 202 1.18e-27 100
Msa0675010 MtrunA17_Chr1g0195561 41.441 111 59 2 1 109 83 189 4.04e-25 93.6
Msa0675010 MtrunA17_Chr7g0269361 39.450 109 64 1 1 109 84 190 9.05e-24 90.1
Msa0675010 MtrunA17_Chr2g0301381 44.643 112 50 2 2 109 86 189 4.03e-23 88.6
Msa0675010 MtrunA17_Chr4g0067891 44.554 101 46 2 13 104 100 199 1.50e-21 84.7
Msa0675010 MtrunA17_Chr8g0391341 44.554 101 46 2 13 104 109 208 3.09e-21 84.0
Msa0675010 MtrunA17_Chr4g0070161 37.037 108 68 0 2 109 92 199 9.37e-19 77.4
Msa0675010 MtrunA17_Chr4g0018231 36.937 111 65 2 2 107 92 202 4.11e-16 70.5
Msa0675010 MtrunA17_Chr1g0147051 38.095 105 51 2 11 103 141 243 7.99e-16 71.6
Msa0675010 MtrunA17_Chr7g0272141 38.235 102 54 3 10 107 99 195 2.63e-15 70.1
Msa0675010 MtrunA17_Chr3g0079371 36.364 88 55 1 18 104 136 223 9.10e-15 67.4
Msa0675010 MtrunA17_Chr3g0126771 36.697 109 61 2 2 104 129 235 2.28e-14 67.8
Msa0675010 MtrunA17_Chr4g0011681 37.500 104 58 2 11 108 97 199 3.04e-14 65.9
Msa0675010 MtrunA17_Chr4g0011721 37.500 104 58 2 11 108 97 199 3.07e-14 65.5
Msa0675010 MtrunA17_Chr4g0011701 37.500 104 58 2 11 108 97 199 3.30e-14 65.5
Msa0675010 MtrunA17_Chr4g0011711 37.500 104 58 2 11 108 97 199 3.31e-14 65.5
Msa0675010 MtrunA17_Chr1g0192311 30.693 101 64 2 11 109 118 214 4.19e-13 63.9
Msa0675010 MtrunA17_Chr1g0192281 30.693 101 64 2 11 109 118 214 4.19e-13 63.9
Msa0675010 MtrunA17_Chr4g0069891 31.579 95 61 1 14 108 143 233 1.53e-11 59.7
Msa0675010 MtrunA17_Chr7g0272151 28.713 101 66 2 11 109 117 213 1.70e-11 59.3
Msa0675010 MtrunA17_Chr8g0345281 29.703 101 70 1 8 107 127 227 1.86e-11 59.3
Msa0675010 MtrunA17_Chr2g0301941 30.851 94 61 1 14 107 141 230 1.90e-11 59.3
Msa0675010 MtrunA17_Chr1g0192321 31.633 98 61 2 12 107 115 208 2.11e-11 59.3
Msa0675010 MtrunA17_Chr4g0067611 33.333 108 66 2 2 107 106 209 2.46e-11 58.9
Msa0675010 MtrunA17_Chr7g0230391 36.957 92 52 2 18 104 119 209 4.44e-11 57.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0675010 AT5G62360.1 57.843 102 43 0 10 111 102 203 8.14e-39 129
Msa0675010 AT1G62770.1 51.515 99 44 1 13 107 98 196 9.96e-30 105
Msa0675010 AT1G62760.1 48.182 110 52 2 4 110 201 308 2.14e-26 99.8
Msa0675010 AT5G62350.1 43.243 111 59 1 1 107 89 199 2.94e-25 94.4
Msa0675010 AT5G20740.1 45.370 108 53 2 9 111 92 198 2.35e-24 92.4
Msa0675010 AT5G20740.2 45.370 108 53 2 9 111 126 232 5.23e-24 92.0
Msa0675010 AT4G25260.1 39.474 114 65 1 1 110 88 201 6.47e-23 88.6
Msa0675010 AT1G70720.2 42.000 100 53 1 13 107 107 206 5.13e-22 86.3
Msa0675010 AT1G70720.1 42.000 100 53 1 13 107 95 194 5.64e-22 85.9
Msa0675010 AT2G01610.1 43.564 101 46 1 15 104 114 214 1.29e-21 85.5
Msa0675010 AT1G23205.1 43.564 101 52 2 9 105 99 198 1.84e-21 84.7
Msa0675010 AT3G47380.1 42.593 108 58 1 4 107 92 199 2.42e-21 84.3
Msa0675010 AT3G62820.1 43.299 97 52 2 13 107 95 190 6.02e-20 80.5
Msa0675010 AT4G25250.1 39.216 102 60 1 4 103 92 193 7.02e-20 80.5
Msa0675010 AT5G51520.1 41.509 106 60 2 2 106 98 202 2.16e-19 79.3
Msa0675010 AT1G14890.1 45.263 95 47 2 15 104 118 212 1.38e-18 77.4
Msa0675010 AT4G12390.1 41.964 112 60 1 4 110 93 204 4.99e-18 75.9
Msa0675010 AT2G47670.1 42.553 94 54 0 14 107 111 204 9.46e-18 75.1
Msa0675010 AT3G14310.1 31.429 105 71 1 4 107 113 217 3.53e-16 73.2
Msa0675010 AT1G53830.1 31.429 105 71 1 4 107 116 220 1.11e-15 71.6
Msa0675010 AT5G53370.1 39.806 103 57 3 2 103 126 224 3.85e-15 70.1
Msa0675010 AT5G53370.2 40.196 102 56 3 2 102 162 259 3.99e-15 70.1
Msa0675010 AT4G00080.1 33.000 100 60 2 10 103 98 196 1.21e-12 61.6
Msa0675010 AT3G49220.2 38.298 94 52 2 10 103 191 278 4.21e-12 61.6
Msa0675010 AT3G49220.1 38.298 94 52 2 10 103 146 233 4.25e-12 61.6
Msa0675010 AT5G27870.1 27.723 101 71 1 11 111 115 213 6.85e-12 60.8
Msa0675010 AT3G49220.3 37.895 95 53 2 10 104 191 279 1.25e-11 60.1
Msa0675010 AT3G05610.1 30.693 101 64 2 13 111 118 214 2.83e-11 59.3

Find 25 sgRNAs with CRISPR-Local

Find 24 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCATAGCTGCAGCCTCTCT+TGG 0.298648 4_4:+23135029 None:intergenic
CCGAAGGTCTATCGATGAAA+TGG 0.365879 4_4:-23134967 Msa0675010:CDS
GTCAACTCTCTCAAAAGGTC+AGG 0.407740 4_4:-23135060 Msa0675010:CDS
CAATGAGTGATGTACAAACT+TGG 0.414535 4_4:-23134914 Msa0675010:CDS
TCATAGCTGCAGCCTCTCTT+GGG 0.426216 4_4:+23135030 None:intergenic
TTGGGTTAGTGCTGCTTTGA+CGG 0.427334 4_4:-23134895 Msa0675010:CDS
AAGAGAGGCTGCAGCTATGA+AGG 0.471251 4_4:-23135027 Msa0675010:CDS
TTGATGTCAACTCTCTCAAA+AGG 0.498369 4_4:-23135065 Msa0675010:CDS
CGAAGGTCTATCGATGAAAT+GGG 0.502061 4_4:-23134966 Msa0675010:CDS
CCATTTCATCGATAGACCTT+CGG 0.513898 4_4:+23134967 None:intergenic
ATGTTAAGACAGTGGTGAGA+GGG 0.549785 4_4:-23134824 Msa0675010:CDS
AATGTTAAGACAGTGGTGAG+AGG 0.559999 4_4:-23134825 Msa0675010:CDS
ATTTCAAGAGATTAGTGCTA+CGG 0.561546 4_4:-23134850 Msa0675010:CDS
TCAACTCTCTCAAAAGGTCA+GGG 0.566267 4_4:-23135059 Msa0675010:CDS
CAAAATTTGATGTACTAAGA+CGG 0.571253 4_4:+23134943 None:intergenic
AATGAGTGATGTACAAACTT+GGG 0.584779 4_4:-23134913 Msa0675010:CDS
AATAAACTTTCCACTTCACA+AGG 0.605086 4_4:-23134753 Msa0675010:CDS
ACTAATCTCTTGAAATCCGT+CGG 0.615355 4_4:+23134857 None:intergenic
GATCAAAACACTTGTACCGA+CGG 0.623780 4_4:-23134873 Msa0675010:CDS
ATTCCGTCGATGAACTCCGA+AGG 0.643719 4_4:-23134983 Msa0675010:CDS
CAACTCTCTCAAAAGGTCAG+GGG 0.648709 4_4:-23135058 Msa0675010:CDS
TCAGGGGATGAACCCAAGAG+AGG 0.650942 4_4:-23135042 Msa0675010:CDS
AGACCTTCGGAGTTCATCGA+CGG 0.662948 4_4:+23134980 None:intergenic
AGCTATGAAGGATTGCGTCG+AGG 0.696363 4_4:-23135015 Msa0675010:CDS
TACGGAGAATGTTAAGACAG+TGG 0.702949 4_4:-23134832 Msa0675010:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! CAAAATTTGATGTACTAAGA+CGG + chr4_4:23134871-23134890 None:intergenic 25.0%
AATGAGTGATGTACAAACTT+GGG - chr4_4:23134898-23134917 Msa0675010:CDS 30.0%
ATTTCAAGAGATTAGTGCTA+CGG - chr4_4:23134961-23134980 Msa0675010:CDS 30.0%
AATAAACTTTCCACTTCACA+AGG - chr4_4:23135058-23135077 Msa0675010:CDS 30.0%
CAATGAGTGATGTACAAACT+TGG - chr4_4:23134897-23134916 Msa0675010:CDS 35.0%
ACTAATCTCTTGAAATCCGT+CGG + chr4_4:23134957-23134976 None:intergenic 35.0%
TCAACTCTCTCAAAAGGTCA+GGG - chr4_4:23134752-23134771 Msa0675010:CDS 40.0%
CCATTTCATCGATAGACCTT+CGG + chr4_4:23134847-23134866 None:intergenic 40.0%
CGAAGGTCTATCGATGAAAT+GGG - chr4_4:23134845-23134864 Msa0675010:CDS 40.0%
GATCAAAACACTTGTACCGA+CGG - chr4_4:23134938-23134957 Msa0675010:CDS 40.0%
TACGGAGAATGTTAAGACAG+TGG - chr4_4:23134979-23134998 Msa0675010:CDS 40.0%
AATGTTAAGACAGTGGTGAG+AGG - chr4_4:23134986-23135005 Msa0675010:CDS 40.0%
ATGTTAAGACAGTGGTGAGA+GGG - chr4_4:23134987-23135006 Msa0675010:CDS 40.0%
GTCAACTCTCTCAAAAGGTC+AGG - chr4_4:23134751-23134770 Msa0675010:CDS 45.0%
CAACTCTCTCAAAAGGTCAG+GGG - chr4_4:23134753-23134772 Msa0675010:CDS 45.0%
CCGAAGGTCTATCGATGAAA+TGG - chr4_4:23134844-23134863 Msa0675010:CDS 45.0%
!! TTGGGTTAGTGCTGCTTTGA+CGG - chr4_4:23134916-23134935 Msa0675010:CDS 45.0%
TCATAGCTGCAGCCTCTCTT+GGG + chr4_4:23134784-23134803 None:intergenic 50.0%
TTCATAGCTGCAGCCTCTCT+TGG + chr4_4:23134785-23134804 None:intergenic 50.0%
AAGAGAGGCTGCAGCTATGA+AGG - chr4_4:23134784-23134803 Msa0675010:CDS 50.0%
AGCTATGAAGGATTGCGTCG+AGG - chr4_4:23134796-23134815 Msa0675010:CDS 50.0%
ATTCCGTCGATGAACTCCGA+AGG - chr4_4:23134828-23134847 Msa0675010:CDS 50.0%
AGACCTTCGGAGTTCATCGA+CGG + chr4_4:23134834-23134853 None:intergenic 50.0%
TCAGGGGATGAACCCAAGAG+AGG - chr4_4:23134769-23134788 Msa0675010:CDS 55.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 23134749 23135084 23134749 ID=Msa0675010;Name=Msa0675010
chr4_4 mRNA 23134749 23135084 23134749 ID=Msa0675010-mRNA-1;Parent=Msa0675010;Name=Msa0675010-mRNA-1;_AED=0.17;_eAED=0.17;_QI=0|-1|0|1|-1|1|1|0|111
chr4_4 exon 23134749 23135084 23134749 ID=Msa0675010-mRNA-1:exon:6469;Parent=Msa0675010-mRNA-1
chr4_4 CDS 23134749 23135084 23134749 ID=Msa0675010-mRNA-1:cds;Parent=Msa0675010-mRNA-1
Gene Sequence

>Msa0675010

ATGTCAACTCTCTCAAAAGGTCAGGGGATGAACCCAAGAGAGGCTGCAGCTATGAAGGATTGCGTCGAGGTGCTAAGTGATTCCGTCGATGAACTCCGAAGGTCTATCGATGAAATGGGCCGTCTTAGTACATCAAATTTTGAACTAACAATGAGTGATGTACAAACTTGGGTTAGTGCTGCTTTGACGGATCAAAACACTTGTACCGACGGATTTCAAGAGATTAGTGCTACGGAGAATGTTAAGACAGTGGTGAGAGGGAGCATTGTGCAAGTTGCTCAATTGACTAGCAATGCTTTAGCATTGATAAATAAACTTTCCACTTCACAAGGTTAA

Protein sequence

>Msa0675010

MSTLSKGQGMNPREAAAMKDCVEVLSDSVDELRRSIDEMGRLSTSNFELTMSDVQTWVSAALTDQNTCTDGFQEISATENVKTVVRGSIVQVAQLTSNALALINKLSTSQG*