AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1216350

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1216350 MtrunA17_Chr4g0065631 93.182 44 3 0 1 44 1 44 3.79e-23 86.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 43 sgRNAs with CRISPR-Local

Find 252 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTATTGAATTCGTATGAATT+AGG 0.224326 8_1:+78069128 Msa1216350:five_prime_UTR
AAAGGAAGTTGGATGATTTC+TGG 0.241926 8_1:-78073223 None:intergenic
AGTAAACTACCTGGTCAATT+TGG 0.246911 8_1:-78069269 None:intergenic
AATTCAGCATTTCATGAAAT+AGG 0.271395 8_1:-78072340 None:intergenic
AATTCATAGTTGTTATAAAC+TGG 0.281008 8_1:-78072300 None:intergenic
AATTCGTATGAATTAGGATA+TGG 0.302466 8_1:+78069134 Msa1216350:exon
AAATAAAATCATTCTTATTC+CGG 0.302899 8_1:-78073318 None:intergenic
AAGACAAGGCAGCATGAGTT+AGG 0.338230 8_1:+78072240 Msa1216350:three_prime_UTR
TGATCGAGATAATCAAATTC+TGG 0.348778 8_1:-78069080 None:intergenic
CCAACTTCCTTTGAAGAAGA+TGG 0.360582 8_1:+78073234 Msa1216350:three_prime_UTR
TCCATGTTTCTATAGTGTTT+AGG 0.362822 8_1:+78072381 Msa1216350:three_prime_UTR
AAGGAAGTTGGATGATTTCT+GGG 0.367425 8_1:-78073222 None:intergenic
TGTGAAGACATCGAAGTGTC+TGG 0.378386 8_1:+78072151 Msa1216350:intron
AGGAATATTATCCTCTTTCA+AGG 0.387832 8_1:-78069244 None:intergenic
CCATCTTCTTCAAAGGAAGT+TGG 0.421879 8_1:-78073234 None:intergenic
TTTGAATAAATCCTTGAAAG+AGG 0.426736 8_1:+78069233 Msa1216350:CDS
AATTCCATCCATGGAGTCAT+AGG 0.439906 8_1:+78073376 Msa1216350:three_prime_UTR
CCGGCGTAAATAACTTATCA+AGG 0.458031 8_1:-78073299 None:intergenic
GGCACTGAAACTTCATCCTC+AGG 0.479293 8_1:+78072267 Msa1216350:three_prime_UTR
CAACTTCCTTTGAAGAAGAT+GGG 0.493093 8_1:+78073235 Msa1216350:three_prime_UTR
TGAACTGATAATCAACGAAA+AGG 0.503651 8_1:-78069040 None:intergenic
CAGCATTTCATGAAATAGGC+TGG 0.510831 8_1:-78072336 None:intergenic
CCTTCTCCCATCTTCTTCAA+AGG 0.517870 8_1:-78073241 None:intergenic
AGGCAGCATGAGTTAGGGAT+TGG 0.539652 8_1:+78072246 Msa1216350:three_prime_UTR
AACGTGGTTAATTCCATCCA+TGG 0.540181 8_1:+78073367 Msa1216350:three_prime_UTR
AGACAAGGCAGCATGAGTTA+GGG 0.561229 8_1:+78072241 Msa1216350:three_prime_UTR
GTGAAGACATCGAAGTGTCT+GGG 0.563144 8_1:+78072152 Msa1216350:intron
TGCACCTATGACTCCATGGA+TGG 0.570427 8_1:-78073380 None:intergenic
TCCTAAACACTATAGAAACA+TGG 0.584437 8_1:-78072382 None:intergenic
TATTCCTTGCCAAATTGACC+AGG 0.585093 8_1:+78069260 Msa1216350:CDS
AACTGGGACTTCCGCACCTG+AGG 0.588074 8_1:-78072283 None:intergenic
GTAGATGATTAGTGTGACTC+TGG 0.589651 8_1:+78072207 Msa1216350:three_prime_UTR
ATTCATAGTTGTTATAAACT+GGG 0.590095 8_1:-78072299 None:intergenic
AATTTGCACCTATGACTCCA+TGG 0.592048 8_1:-78073384 None:intergenic
CCTTTGAAGAAGATGGGAGA+AGG 0.594462 8_1:+78073241 Msa1216350:three_prime_UTR
CTGGCAATCACGTGAAGACA+AGG 0.595486 8_1:+78072226 Msa1216350:three_prime_UTR
CGGCGTAAATAACTTATCAA+GGG 0.599107 8_1:-78073298 None:intergenic
TGCCATAAACGCAATACTTG+AGG 0.602797 8_1:+78073164 Msa1216350:intron
CCTTGATAAGTTATTTACGC+CGG 0.607125 8_1:+78073299 Msa1216350:three_prime_UTR
ACTACCTGGTCAATTTGGCA+AGG 0.624601 8_1:-78069264 None:intergenic
AACATGATGTTGATCAGACA+AGG 0.651098 8_1:+78072180 Msa1216350:CDS
TGAAACTTCATCCTCAGGTG+CGG 0.651972 8_1:+78072272 Msa1216350:three_prime_UTR
AAAGTGGACTAGTCATAACG+TGG 0.742351 8_1:+78073351 Msa1216350:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATATATATATATATATAT+TGG - chr8_1:78071332-78071351 None:intergenic 0.0%
!!! CATCTTAAATATATTTTAAA+TGG + chr8_1:78071400-78071419 Msa1216350:intron 10.0%
!!! TTGCAATTTTTTTAATATTT+TGG - chr8_1:78072480-78072499 None:intergenic 10.0%
!! AAAATACACTATAAGATATA+TGG - chr8_1:78070202-78070221 None:intergenic 15.0%
!!! AATTTTGTCTTAAGTTTTTT+AGG + chr8_1:78070383-78070402 Msa1216350:intron 15.0%
!!! ATCATATCTGTAATTTTAAA+GGG - chr8_1:78070665-78070684 None:intergenic 15.0%
!!! AATCATATCTGTAATTTTAA+AGG - chr8_1:78070666-78070685 None:intergenic 15.0%
!! ATATATATATTGGTATGTTA+AGG - chr8_1:78071322-78071341 None:intergenic 15.0%
!!! AAATCATAAATGTATTTTGA+TGG - chr8_1:78071446-78071465 None:intergenic 15.0%
!!! AATATCTAAATTTTAATGCA+TGG + chr8_1:78071863-78071882 Msa1216350:intron 15.0%
!!! AGTACTCAATTTTTAAATAA+CGG + chr8_1:78072502-78072521 Msa1216350:intron 15.0%
!! AAATAAAATCATTCTTATTC+CGG - chr8_1:78073321-78073340 None:intergenic 15.0%
!!! AAGAATGATTTTATTTTTTC+TGG + chr8_1:78073325-78073344 Msa1216350:three_prime_UTR 15.0%
!! TTATTGAATTCGTATGAATT+AGG + chr8_1:78069128-78069147 Msa1216350:five_prime_UTR 20.0%
!!! ACAAATTGCATTAATTTTGA+AGG + chr8_1:78069203-78069222 Msa1216350:CDS 20.0%
!! AAATAACGATGAATGTTATT+TGG + chr8_1:78069334-78069353 Msa1216350:intron 20.0%
!! TATAACTTTATAGACGAAAT+CGG - chr8_1:78069473-78069492 None:intergenic 20.0%
!!! GGAAATTGATAATATCAATA+AGG - chr8_1:78069753-78069772 None:intergenic 20.0%
!! ATTATCAATTTCCATGTTTA+TGG + chr8_1:78069760-78069779 Msa1216350:intron 20.0%
!! TAAAGAAATAACGTTAAAGT+AGG + chr8_1:78069823-78069842 Msa1216350:intron 20.0%
!! AAAGAAATAACGTTAAAGTA+GGG + chr8_1:78069824-78069843 Msa1216350:intron 20.0%
!!! AATTTTTATTTTGGTGACAA+TGG + chr8_1:78069919-78069938 Msa1216350:intron 20.0%
!!! TATCTTATAGTGTATTTTGT+AGG + chr8_1:78070204-78070223 Msa1216350:intron 20.0%
!! ATAGTAACAATAATGTTTAG+TGG - chr8_1:78071642-78071661 None:intergenic 20.0%
!! AACAAATTAGATACAATCAA+TGG - chr8_1:78072129-78072148 None:intergenic 20.0%
!! ATTCATAGTTGTTATAAACT+GGG - chr8_1:78072302-78072321 None:intergenic 20.0%
!! AATTCATAGTTGTTATAAAC+TGG - chr8_1:78072303-78072322 None:intergenic 20.0%
!!! ATTTTTCTGTTTCATATTCA+AGG + chr8_1:78072560-78072579 Msa1216350:intron 20.0%
!!! TTTTTCTGTTTCATATTCAA+GGG + chr8_1:78072561-78072580 Msa1216350:intron 20.0%
!!! TTATTTTTTCTGGAAAAAAG+TGG + chr8_1:78073335-78073354 Msa1216350:three_prime_UTR 20.0%
! AATTCGTATGAATTAGGATA+TGG + chr8_1:78069134-78069153 Msa1216350:exon 25.0%
! TTTGAATAAATCCTTGAAAG+AGG + chr8_1:78069233-78069252 Msa1216350:CDS 25.0%
! TTCGTCTATAAAGTTATAGA+TGG + chr8_1:78069475-78069494 Msa1216350:intron 25.0%
! TTTACACTAGTAAATGGATA+GGG + chr8_1:78069559-78069578 Msa1216350:intron 25.0%
!! TTTTTGCTTATATATGTGAC+CGG - chr8_1:78069610-78069629 None:intergenic 25.0%
! TGCACATTGTATTTAATGTA+AGG + chr8_1:78069794-78069813 Msa1216350:intron 25.0%
!!! CGGGTATGTAATTTTTATTT+TGG + chr8_1:78069910-78069929 Msa1216350:intron 25.0%
!! TGATCTTTAAAAGTTTAGCA+TGG + chr8_1:78070010-78070029 Msa1216350:intron 25.0%
!! GATCTTTAAAAGTTTAGCAT+GGG + chr8_1:78070011-78070030 Msa1216350:intron 25.0%
! ACATCTATTATAAAGCTATC+TGG - chr8_1:78070231-78070250 None:intergenic 25.0%
!! ACAAAAGTGTAACTTTAGTA+TGG - chr8_1:78070427-78070446 None:intergenic 25.0%
!! TACTAAAGTTACACTTTTGT+TGG + chr8_1:78070427-78070446 Msa1216350:intron 25.0%
!!! TTTGAAGTGTTGTTTTAAGT+TGG - chr8_1:78070484-78070503 None:intergenic 25.0%
! ACAAGGTTAATCTAATGAAA+CGG - chr8_1:78070597-78070616 None:intergenic 25.0%
!!! CAATTATAAAAGCATCAACA+AGG - chr8_1:78070614-78070633 None:intergenic 25.0%
! AAACGCTTTATAGTATGAAT+TGG + chr8_1:78070827-78070846 Msa1216350:intron 25.0%
! AATATGTAGAGTTCAAAGTT+AGG + chr8_1:78070903-78070922 Msa1216350:intron 25.0%
! ATATGTAGAGTTCAAAGTTA+GGG + chr8_1:78070904-78070923 Msa1216350:intron 25.0%
!! ATTATGTCCTTGTTTACTTT+GGG + chr8_1:78070952-78070971 Msa1216350:intron 25.0%
!!! TTTACTACCTCCTTTTTTAA+TGG + chr8_1:78071012-78071031 Msa1216350:intron 25.0%
! AATATGACCATTAAAAAAGG+AGG - chr8_1:78071022-78071041 None:intergenic 25.0%
!!! TCCTTTTTTAATGGTCATAT+TGG + chr8_1:78071021-78071040 Msa1216350:intron 25.0%
! TCCAATATGACCATTAAAAA+AGG - chr8_1:78071025-78071044 None:intergenic 25.0%
!! AAATTAGAACCAAGTTTTTC+TGG - chr8_1:78071106-78071125 None:intergenic 25.0%
!!! TAAATGTATTTTGATGGCTA+TGG - chr8_1:78071440-78071459 None:intergenic 25.0%
! TTAATGCAATTCTGTCAAAA+TGG + chr8_1:78071904-78071923 Msa1216350:intron 25.0%
! TATCGATAACAAAAGAAATG+TGG - chr8_1:78072052-78072071 None:intergenic 25.0%
! AATTCAGCATTTCATGAAAT+AGG - chr8_1:78072343-78072362 None:intergenic 25.0%
! TCTATAGTGTTTAGGAAAAA+TGG + chr8_1:78072389-78072408 Msa1216350:three_prime_UTR 25.0%
!! TCAAAAACTCAAGTACTTTT+AGG - chr8_1:78072592-78072611 None:intergenic 25.0%
! ACTCAAACTGATAAAAGAAA+AGG + chr8_1:78072688-78072707 Msa1216350:intron 25.0%
! TCGCAAGACAATTTAATTAA+AGG + chr8_1:78072908-78072927 Msa1216350:intron 25.0%
!! CTCTTTAACTTACGTTAATT+GGG - chr8_1:78072950-78072969 None:intergenic 25.0%
! ATATGTTATTGCACTAAGAA+TGG - chr8_1:78073115-78073134 None:intergenic 25.0%
TGAACTGATAATCAACGAAA+AGG - chr8_1:78069043-78069062 None:intergenic 30.0%
TGATCGAGATAATCAAATTC+TGG - chr8_1:78069083-78069102 None:intergenic 30.0%
AGGAATATTATCCTCTTTCA+AGG - chr8_1:78069247-78069266 None:intergenic 30.0%
! ACGATGAATGTTATTTGGTT+AGG + chr8_1:78069339-78069358 Msa1216350:intron 30.0%
! CGATGAATGTTATTTGGTTA+GGG + chr8_1:78069340-78069359 Msa1216350:intron 30.0%
GTTTACACTAGTAAATGGAT+AGG + chr8_1:78069558-78069577 Msa1216350:intron 30.0%
CATTGAATGACTAATGTACA+TGG + chr8_1:78069645-78069664 Msa1216350:intron 30.0%
GTTGTACTAGAAAGATGATT+TGG + chr8_1:78069711-78069730 Msa1216350:intron 30.0%
ACTCAAATAGTCCATAAACA+TGG - chr8_1:78069774-78069793 None:intergenic 30.0%
TTAAAGATCAAAGCACACAA+TGG - chr8_1:78070000-78070019 None:intergenic 30.0%
AACTTTAATGACAAGCAACA+TGG - chr8_1:78070337-78070356 None:intergenic 30.0%
! ACACTTTTGTTGGAAAATTG+AGG + chr8_1:78070437-78070456 Msa1216350:intron 30.0%
! TTTTATAATTGAGTGCCCTT+GGG + chr8_1:78070623-78070642 Msa1216350:intron 30.0%
! GATTATGTCCTTGTTTACTT+TGG + chr8_1:78070951-78070970 Msa1216350:intron 30.0%
TTAGAGTAACCAGAAAAACT+TGG + chr8_1:78071094-78071113 Msa1216350:intron 30.0%
! GACTTTGAGAAATCTCTTAA+AGG + chr8_1:78071265-78071284 Msa1216350:intron 30.0%
CATGGTAAAGGAAGTAAAAA+GGG + chr8_1:78071604-78071623 Msa1216350:intron 30.0%
ATACTGCTGTTCTGTTTATA+TGG + chr8_1:78071994-78072013 Msa1216350:intron 30.0%
ATTAGTGAAGAAAATGAAGG+GGG - chr8_1:78072024-78072043 None:intergenic 30.0%
CATTAGTGAAGAAAATGAAG+GGG - chr8_1:78072025-78072044 None:intergenic 30.0%
CCATTAGTGAAGAAAATGAA+GGG - chr8_1:78072026-78072045 None:intergenic 30.0%
TCCATTAGTGAAGAAAATGA+AGG - chr8_1:78072027-78072046 None:intergenic 30.0%
! TCCATGTTTCTATAGTGTTT+AGG + chr8_1:78072381-78072400 Msa1216350:three_prime_UTR 30.0%
TCCTAAACACTATAGAAACA+TGG - chr8_1:78072385-78072404 None:intergenic 30.0%
TTTAGGAAAAATGGTCTGTT+AGG + chr8_1:78072398-78072417 Msa1216350:three_prime_UTR 30.0%
! ATTAACAACTTTTGTTCGAG+AGG + chr8_1:78072425-78072444 Msa1216350:three_prime_UTR 30.0%
! TTGAGTATTCTTGAGCTTTT+TGG + chr8_1:78072534-78072553 Msa1216350:intron 30.0%
!! AAGTACTTGAGTTTTTGAAG+TGG + chr8_1:78072594-78072613 Msa1216350:intron 30.0%
TAATTAAATTGTCTTGCGAG+TGG - chr8_1:78072907-78072926 None:intergenic 30.0%
! CCTCTTTAACTTACGTTAAT+TGG - chr8_1:78072951-78072970 None:intergenic 30.0%
CCAATTAACGTAAGTTAAAG+AGG + chr8_1:78072948-78072967 Msa1216350:intron 30.0%
ATTAACGTAAGTTAAAGAGG+AGG + chr8_1:78072951-78072970 Msa1216350:intron 30.0%
AGTTACAAGAAATCTCTGTT+TGG - chr8_1:78073038-78073057 None:intergenic 30.0%
AGTAAACTACCTGGTCAATT+TGG - chr8_1:78069272-78069291 None:intergenic 35.0%
GCATGATTAAGTAAACTACC+TGG - chr8_1:78069281-78069300 None:intergenic 35.0%
! ATTTGGTTAGGGTTTGTTTC+TGG + chr8_1:78069351-78069370 Msa1216350:intron 35.0%
! TTTGGTTAGGGTTTGTTTCT+GGG + chr8_1:78069352-78069371 Msa1216350:intron 35.0%
!!! TGTTACTCACATTTTGACTG+TGG + chr8_1:78069420-78069439 Msa1216350:intron 35.0%
GACAGGTTTACACTAGTAAA+TGG + chr8_1:78069553-78069572 Msa1216350:intron 35.0%
ATGTGACCAGATGCAATATA+AGG + chr8_1:78069675-78069694 Msa1216350:intron 35.0%
TAACGTTAAAGTAGGGCAAA+AGG + chr8_1:78069831-78069850 Msa1216350:intron 35.0%
!! AGTTTTACAGAACACCGATT+AGG + chr8_1:78069880-78069899 Msa1216350:intron 35.0%
ATTGATGAAGCCAAAAGCAA+TGG + chr8_1:78070519-78070538 Msa1216350:intron 35.0%
!!! GTTTTGGTTCATTGTTATGC+TGG + chr8_1:78070549-78070568 Msa1216350:intron 35.0%
! CTTTTATAATTGAGTGCCCT+TGG + chr8_1:78070622-78070641 Msa1216350:intron 35.0%
TTTATAATTGAGTGCCCTTG+GGG + chr8_1:78070624-78070643 Msa1216350:intron 35.0%
GAAGAATGCACTTCAATTGT+TGG - chr8_1:78070790-78070809 None:intergenic 35.0%
! TTTGATGGCTATGGCTATAT+AGG - chr8_1:78071431-78071450 None:intergenic 35.0%
GCATGGTAAAGGAAGTAAAA+AGG + chr8_1:78071603-78071622 Msa1216350:intron 35.0%
!! AGCTTCATGGGTTTTTCAAA+TGG + chr8_1:78071927-78071946 Msa1216350:intron 35.0%
! CCCTTCATTTTCTTCACTAA+TGG + chr8_1:78072023-78072042 Msa1216350:intron 35.0%
AACATGATGTTGATCAGACA+AGG + chr8_1:78072180-78072199 Msa1216350:CDS 35.0%
GATATTGGACTTGCCTAATT+GGG - chr8_1:78072646-78072665 None:intergenic 35.0%
CAATTAGGCAAGTCCAATAT+CGG + chr8_1:78072645-78072664 Msa1216350:intron 35.0%
TTTGAGTCAACAATGAGTGT+GGG - chr8_1:78072675-78072694 None:intergenic 35.0%
ATCGTACGTCATATTTGAGA+TGG - chr8_1:78072876-78072895 None:intergenic 35.0%
ATCTCAAATATGACGTACGA+TGG + chr8_1:78072875-78072894 Msa1216350:intron 35.0%
ACTTACGTTAATTGGGATAG+TGG - chr8_1:78072943-78072962 None:intergenic 35.0%
GTACTCAAATGTTGTTGTCT+TGG + chr8_1:78073077-78073096 Msa1216350:intron 35.0%
AGTGCAATAACATATCACAC+TGG + chr8_1:78073120-78073139 Msa1216350:intron 35.0%
GTGCAATAACATATCACACT+GGG + chr8_1:78073121-78073140 Msa1216350:intron 35.0%
TGCAATAACATATCACACTG+GGG + chr8_1:78073122-78073141 Msa1216350:intron 35.0%
AAGGAAGTTGGATGATTTCT+GGG - chr8_1:78073225-78073244 None:intergenic 35.0%
AAAGGAAGTTGGATGATTTC+TGG - chr8_1:78073226-78073245 None:intergenic 35.0%
! CAACTTCCTTTGAAGAAGAT+GGG + chr8_1:78073235-78073254 Msa1216350:three_prime_UTR 35.0%
CGGCGTAAATAACTTATCAA+GGG - chr8_1:78073301-78073320 None:intergenic 35.0%
CCTTGATAAGTTATTTACGC+CGG + chr8_1:78073299-78073318 Msa1216350:three_prime_UTR 35.0%
TATTCCTTGCCAAATTGACC+AGG + chr8_1:78069260-78069279 Msa1216350:CDS 40.0%
!! TGTTGGTTCTGCTACAAACA+AGG + chr8_1:78069378-78069397 Msa1216350:intron 40.0%
ACTTTATAGACGAAATCGGC+AGG - chr8_1:78069469-78069488 None:intergenic 40.0%
GTAAATGGATAGGGTGTGTT+TGG + chr8_1:78069568-78069587 Msa1216350:intron 40.0%
TGCTTATATATGTGACCGGA+GGG - chr8_1:78069606-78069625 None:intergenic 40.0%
TTGCTTATATATGTGACCGG+AGG - chr8_1:78069607-78069626 None:intergenic 40.0%
GTTAAAGTAGGGCAAAAGGA+AGG + chr8_1:78069835-78069854 Msa1216350:intron 40.0%
ACACAATGGTAATCGAGTCA+GGG - chr8_1:78069986-78070005 None:intergenic 40.0%
TAGTACTCGACACATCCAAT+AGG - chr8_1:78070129-78070148 None:intergenic 40.0%
! AACACTTCCACCATTGCTTT+TGG - chr8_1:78070532-78070551 None:intergenic 40.0%
!!! AAGCAATGGTGGAAGTGTTT+TGG + chr8_1:78070533-78070552 Msa1216350:intron 40.0%
ATTGTTATGCTGGAAGATCG+AGG + chr8_1:78070559-78070578 Msa1216350:intron 40.0%
TCACATGCCCAAAGTAAACA+AGG - chr8_1:78070962-78070981 None:intergenic 40.0%
CATCAGATATGCCACAATGA+TGG - chr8_1:78071149-78071168 None:intergenic 40.0%
TGGCATATCTGATGAAGTCT+CGG + chr8_1:78071155-78071174 Msa1216350:intron 40.0%
ATGTTGCAGAGCTTCAGATA+AGG - chr8_1:78071188-78071207 None:intergenic 40.0%
TGACTAATGAAACCGCGATT+TGG - chr8_1:78071238-78071257 None:intergenic 40.0%
TCTGTCAAAATGGAGCTTCA+TGG + chr8_1:78071914-78071933 Msa1216350:intron 40.0%
CTGTCAAAATGGAGCTTCAT+GGG + chr8_1:78071915-78071934 Msa1216350:intron 40.0%
GTAGATGATTAGTGTGACTC+TGG + chr8_1:78072207-78072226 Msa1216350:three_prime_UTR 40.0%
CAGCATTTCATGAAATAGGC+TGG - chr8_1:78072339-78072358 None:intergenic 40.0%
!! TTGGTTTTCGTAAGTGCTAC+TGG - chr8_1:78072461-78072480 None:intergenic 40.0%
TTTCCAAGCTGAACCCAATT+AGG + chr8_1:78072630-78072649 Msa1216350:intron 40.0%
CGATATTGGACTTGCCTAAT+TGG - chr8_1:78072647-78072666 None:intergenic 40.0%
GTTTGAGTCAACAATGAGTG+TGG - chr8_1:78072676-78072695 None:intergenic 40.0%
!! GGTTTTAATCAGAGTGTGCA+CGG - chr8_1:78072749-78072768 None:intergenic 40.0%
AAAGGTAGACAAGATGTGGT+CGG - chr8_1:78072826-78072845 None:intergenic 40.0%
GTTAAAGAGGAGGCAAATGA+AGG + chr8_1:78072961-78072980 Msa1216350:intron 40.0%
CTCAAATGTTGTTGTCTTGG+CGG + chr8_1:78073080-78073099 Msa1216350:intron 40.0%
TCAAATGTTGTTGTCTTGGC+GGG + chr8_1:78073081-78073100 Msa1216350:intron 40.0%
TGCCATAAACGCAATACTTG+AGG + chr8_1:78073164-78073183 Msa1216350:intron 40.0%
! CACCTCAAGTATTGCGTTTA+TGG - chr8_1:78073169-78073188 None:intergenic 40.0%
CCATCTTCTTCAAAGGAAGT+TGG - chr8_1:78073237-78073256 None:intergenic 40.0%
! CCAACTTCCTTTGAAGAAGA+TGG + chr8_1:78073234-78073253 Msa1216350:three_prime_UTR 40.0%
CCGGCGTAAATAACTTATCA+AGG - chr8_1:78073302-78073321 None:intergenic 40.0%
!! AAAGTGGACTAGTCATAACG+TGG + chr8_1:78073351-78073370 Msa1216350:three_prime_UTR 40.0%
AACGTGGTTAATTCCATCCA+TGG + chr8_1:78073367-78073386 Msa1216350:three_prime_UTR 40.0%
AATTCCATCCATGGAGTCAT+AGG + chr8_1:78073376-78073395 Msa1216350:three_prime_UTR 40.0%
ACTACCTGGTCAATTTGGCA+AGG - chr8_1:78069267-78069286 None:intergenic 45.0%
!! GGTTTGTTTCTGGGTTCTGT+TGG + chr8_1:78069361-78069380 Msa1216350:intron 45.0%
GGTTCTGCTACAAACAAGGT+TGG + chr8_1:78069382-78069401 Msa1216350:intron 45.0%
!!! ACTCACATTTTGACTGTGGC+TGG + chr8_1:78069424-78069443 Msa1216350:intron 45.0%
!!! CTCACATTTTGACTGTGGCT+GGG + chr8_1:78069425-78069444 Msa1216350:intron 45.0%
ATAGACGAAATCGGCAGGAT+GGG - chr8_1:78069464-78069483 None:intergenic 45.0%
TATAGACGAAATCGGCAGGA+TGG - chr8_1:78069465-78069484 None:intergenic 45.0%
AAACCTGTCAGTGTCATGTC+TGG - chr8_1:78069542-78069561 None:intergenic 45.0%
TGGAAATACATCTCTCCCTC+CGG + chr8_1:78069588-78069607 Msa1216350:intron 45.0%
GCTCGACCTTATATTGCATC+TGG - chr8_1:78069684-78069703 None:intergenic 45.0%
AAAGTAGGGCAAAAGGAAGG+AGG + chr8_1:78069838-78069857 Msa1216350:intron 45.0%
AAGTAGGGCAAAAGGAAGGA+GGG + chr8_1:78069839-78069858 Msa1216350:intron 45.0%
! TACAGAACACCGATTAGGAC+AGG + chr8_1:78069885-78069904 Msa1216350:intron 45.0%
! ACAGAACACCGATTAGGACA+GGG + chr8_1:78069886-78069905 Msa1216350:intron 45.0%
GTGACAATGGTGCGTGTTTA+GGG + chr8_1:78069932-78069951 Msa1216350:intron 45.0%
AATGGTAATCGAGTCAGGGT+CGG - chr8_1:78069982-78070001 None:intergenic 45.0%
CACACAATGGTAATCGAGTC+AGG - chr8_1:78069987-78070006 None:intergenic 45.0%
!! CTATCTGAAGTGTCGAGTAG+TGG - chr8_1:78070048-78070067 None:intergenic 45.0%
CACTACTCGACACTTCAGAT+AGG + chr8_1:78070046-78070065 Msa1216350:intron 45.0%
TCTCTCTCTCTCTCTCCTAT+TGG + chr8_1:78070111-78070130 Msa1216350:intron 45.0%
! ATGAGAGTGCACAGCATTTG+AGG + chr8_1:78070310-78070329 Msa1216350:intron 45.0%
TGAGGAAGTTGCTGACAAAG+AGG + chr8_1:78070455-78070474 Msa1216350:intron 45.0%
GATGAAGCCAAAAGCAATGG+TGG + chr8_1:78070522-78070541 Msa1216350:intron 45.0%
AATGTTAGCAAGTGCCCCAA+GGG - chr8_1:78070641-78070660 None:intergenic 45.0%
AAATGTTAGCAAGTGCCCCA+AGG - chr8_1:78070642-78070661 None:intergenic 45.0%
ACAATGATGGTCACACTGGA+AGG - chr8_1:78071136-78071155 None:intergenic 45.0%
TTCCAGTGTGACCATCATTG+TGG + chr8_1:78071135-78071154 Msa1216350:intron 45.0%
GGCATATCTGATGAAGTCTC+GGG + chr8_1:78071156-78071175 Msa1216350:intron 45.0%
GCATATCTGATGAAGTCTCG+GGG + chr8_1:78071157-78071176 Msa1216350:intron 45.0%
GTAATCTAGCAGAGTGTGCA+TGG + chr8_1:78071586-78071605 Msa1216350:intron 45.0%
TAGCAGAGTGTGCATGGTAA+AGG + chr8_1:78071592-78071611 Msa1216350:intron 45.0%
! TGTGAAGACATCGAAGTGTC+TGG + chr8_1:78072151-78072170 Msa1216350:intron 45.0%
! GTGAAGACATCGAAGTGTCT+GGG + chr8_1:78072152-78072171 Msa1216350:intron 45.0%
AAGACAAGGCAGCATGAGTT+AGG + chr8_1:78072240-78072259 Msa1216350:three_prime_UTR 45.0%
AGACAAGGCAGCATGAGTTA+GGG + chr8_1:78072241-78072260 Msa1216350:three_prime_UTR 45.0%
TGAAACTTCATCCTCAGGTG+CGG + chr8_1:78072272-78072291 Msa1216350:three_prime_UTR 45.0%
TTGCCTAATTGGGTTCAGCT+TGG - chr8_1:78072636-78072655 None:intergenic 45.0%
TCTTCTCATCATGTGCTCAC+AGG - chr8_1:78072770-78072789 None:intergenic 45.0%
AAACCTCCAGTCTTGCTTGT+GGG - chr8_1:78072850-78072869 None:intergenic 45.0%
AGACGCTTGTAGACGTTCAT+AGG + chr8_1:78072993-78073012 Msa1216350:intron 45.0%
CCTTCTCCCATCTTCTTCAA+AGG - chr8_1:78073244-78073263 None:intergenic 45.0%
! CCTTTGAAGAAGATGGGAGA+AGG + chr8_1:78073241-78073260 Msa1216350:three_prime_UTR 45.0%
!! AAAAAAAAATTATAAAAGAA+AGG - chr8_1:78071840-78071859 None:intergenic 5.0%
ACACCAGACATGACACTGAC+AGG + chr8_1:78069536-78069555 Msa1216350:intron 50.0%
! AACACCGATTAGGACAGGGT+CGG + chr8_1:78069890-78069909 Msa1216350:intron 50.0%
ATACCCGACCCTGTCCTAAT+CGG - chr8_1:78069897-78069916 None:intergenic 50.0%
GGTGACAATGGTGCGTGTTT+AGG + chr8_1:78069931-78069950 Msa1216350:intron 50.0%
ACAATGGTGCGTGTTTAGGG+AGG + chr8_1:78069935-78069954 Msa1216350:intron 50.0%
GTGTCGAGTACTAGAGAGAG+AGG + chr8_1:78070136-78070155 Msa1216350:intron 50.0%
GTTATGCTGGAAGATCGAGG+AGG + chr8_1:78070562-78070581 Msa1216350:intron 50.0%
ATGATGGTCACACTGGAAGG+TGG - chr8_1:78071133-78071152 None:intergenic 50.0%
TGCCACAATGATGGTCACAC+TGG - chr8_1:78071140-78071159 None:intergenic 50.0%
TCGCGGTTTCATTAGTCAGC+TGG + chr8_1:78071240-78071259 Msa1216350:intron 50.0%
CTGGCAATCACGTGAAGACA+AGG + chr8_1:78072226-78072245 Msa1216350:three_prime_UTR 50.0%
AGGCAGCATGAGTTAGGGAT+TGG + chr8_1:78072246-78072265 Msa1216350:three_prime_UTR 50.0%
! GGCACTGAAACTTCATCCTC+AGG + chr8_1:78072267-78072286 Msa1216350:three_prime_UTR 50.0%
GAGTGTGGGCAATCCGATAT+TGG - chr8_1:78072661-78072680 None:intergenic 50.0%
AGTCGTGGGTAGACAAGAAG+TGG - chr8_1:78072798-78072817 None:intergenic 50.0%
ACTTCTTGTCTACCCACGAC+TGG + chr8_1:78072797-78072816 Msa1216350:intron 50.0%
CTTCTTGTCTACCCACGACT+GGG + chr8_1:78072798-78072817 Msa1216350:intron 50.0%
GGGCAAAGGTAGACAAGATG+TGG - chr8_1:78072830-78072849 None:intergenic 50.0%
GAAACCTCCAGTCTTGCTTG+TGG - chr8_1:78072851-78072870 None:intergenic 50.0%
GGCAAAAGGAAGGAGGGAAG+CGG + chr8_1:78069845-78069864 Msa1216350:intron 55.0%
AAAAGGAAGGAGGGAAGCGG+CGG + chr8_1:78069848-78069867 Msa1216350:intron 55.0%
! ACACCGATTAGGACAGGGTC+GGG + chr8_1:78069891-78069910 Msa1216350:intron 55.0%
ATGGTGCGTGTTTAGGGAGG+CGG + chr8_1:78069938-78069957 Msa1216350:intron 55.0%
CCACAAGCAGCTCCAAATCG+CGG + chr8_1:78071223-78071242 Msa1216350:intron 55.0%
TGTCATCTCCTCTCCACGAC+TGG + chr8_1:78072717-78072736 Msa1216350:intron 55.0%
GTCATCTCCTCTCCACGACT+GGG + chr8_1:78072718-78072737 Msa1216350:intron 55.0%
CCAGTCTTGCTTGTGGGCAA+AGG - chr8_1:78072844-78072863 None:intergenic 55.0%
CCTTTGCCCACAAGCAAGAC+TGG + chr8_1:78072841-78072860 Msa1216350:intron 55.0%
TTGCCCACAAGCAAGACTGG+AGG + chr8_1:78072844-78072863 Msa1216350:intron 55.0%
ACGAAATCGGCAGGATGGGC+AGG - chr8_1:78069460-78069479 None:intergenic 60.0%
TGGTGCGTGTTTAGGGAGGC+GGG + chr8_1:78069939-78069958 Msa1216350:intron 60.0%
! CCGCGATTTGGAGCTGCTTG+TGG - chr8_1:78071226-78071245 None:intergenic 60.0%
AACTGGGACTTCCGCACCTG+AGG - chr8_1:78072286-78072305 None:intergenic 60.0%
GGTAGTACCCAGTCGTGGAG+AGG - chr8_1:78072728-78072747 None:intergenic 60.0%
TGCACGGTAGTACCCAGTCG+TGG - chr8_1:78072733-78072752 None:intergenic 60.0%
TGTGGTCGGAACCCAGTCGT+GGG - chr8_1:78072812-78072831 None:intergenic 60.0%
ATGTGGTCGGAACCCAGTCG+TGG - chr8_1:78072813-78072832 None:intergenic 60.0%
AGGAGGGAAGCGGCGGTAGT+CGG + chr8_1:78069855-78069874 Msa1216350:intron 65.0%
CGTGTTTAGGGAGGCGGGTC+AGG + chr8_1:78069944-78069963 Msa1216350:intron 65.0%
GTTTAGGGAGGCGGGTCAGG+TGG + chr8_1:78069947-78069966 Msa1216350:intron 65.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 78069024 78073401 78069024 ID=Msa1216350;Name=Msa1216350
chr8_1 mRNA 78069024 78073401 78069024 ID=Msa1216350-mRNA-1;Parent=Msa1216350;Name=Msa1216350-mRNA-1;_AED=0.33;_eAED=0.33;_QI=129|0.25|0.6|0.8|0.75|0.6|5|479|63
chr8_1 exon 78069024 78069281 78069024 ID=Msa1216350-mRNA-1:exon:26991;Parent=Msa1216350-mRNA-1
chr8_1 exon 78070405 78070411 78070405 ID=Msa1216350-mRNA-1:exon:26992;Parent=Msa1216350-mRNA-1
chr8_1 exon 78070912 78070919 78070912 ID=Msa1216350-mRNA-1:exon:26993;Parent=Msa1216350-mRNA-1
chr8_1 exon 78072158 78072460 78072158 ID=Msa1216350-mRNA-1:exon:26994;Parent=Msa1216350-mRNA-1
chr8_1 exon 78073178 78073401 78073178 ID=Msa1216350-mRNA-1:exon:26995;Parent=Msa1216350-mRNA-1
chr8_1 five_prime_UTR 78069024 78069152 78069024 ID=Msa1216350-mRNA-1:five_prime_utr;Parent=Msa1216350-mRNA-1
chr8_1 CDS 78069153 78069281 78069153 ID=Msa1216350-mRNA-1:cds;Parent=Msa1216350-mRNA-1
chr8_1 CDS 78070405 78070411 78070405 ID=Msa1216350-mRNA-1:cds;Parent=Msa1216350-mRNA-1
chr8_1 CDS 78070912 78070919 78070912 ID=Msa1216350-mRNA-1:cds;Parent=Msa1216350-mRNA-1
chr8_1 CDS 78072158 78072205 78072158 ID=Msa1216350-mRNA-1:cds;Parent=Msa1216350-mRNA-1
chr8_1 three_prime_UTR 78072206 78072460 78072206 ID=Msa1216350-mRNA-1:three_prime_utr;Parent=Msa1216350-mRNA-1
chr8_1 three_prime_UTR 78073178 78073401 78073178 ID=Msa1216350-mRNA-1:three_prime_utr;Parent=Msa1216350-mRNA-1
Gene Sequence

>Msa1216350

ATGGCTACAACATCAGAAGCTATGAATGAATTCGACGAATCGATGAAGAAACAAATTGCATTAATTTTGAAGGTTGTGCTTTTGAATAAATCCTTGAAAGAGGATAATATTCCTTGCCAAATTGACCAGGCATGTAAGTTCAAAACATCGAAGTGTCTGGGTAGAGAACATGATGTTGATCAGACAAGGTAG
Protein sequence

>Msa1216350

MATTSEAMNEFDESMKKQIALILKVVLLNKSLKEDNIPCQIDQACKFKTSKCLGREHDVDQTR*