AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1291460

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1291460 MtrunA17_Chr8g0365941 95.918 245 10 0 1 245 1 245 6.39e-171 483
Msa1291460 MtrunA17_Chr8g0365941 98.039 102 2 0 245 346 366 467 2.12e-65 214
Msa1291460 MtrunA17_Chr5g0421111 46.154 312 94 11 1 245 1 305 3.55e-54 186
Msa1291460 MtrunA17_Chr5g0421111 67.021 94 30 1 245 338 426 518 2.19e-34 132
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1291460 AT1G68920.1 31.474 251 115 10 17 245 28 243 1.81e-14 74.7
Msa1291460 AT1G68920.1 47.191 89 36 2 245 333 364 441 3.07e-14 73.9
Msa1291460 AT1G68920.4 31.474 251 115 10 17 245 28 243 1.81e-14 74.7
Msa1291460 AT1G68920.4 47.191 89 36 2 245 333 364 441 3.07e-14 73.9
Msa1291460 AT1G68920.2 31.474 251 115 10 17 245 28 243 1.81e-14 74.7
Msa1291460 AT1G68920.2 47.191 89 36 2 245 333 364 441 3.07e-14 73.9
Msa1291460 AT1G68920.3 31.474 251 115 10 17 245 28 243 1.86e-14 74.7
Msa1291460 AT1G68920.3 47.191 89 36 2 245 333 363 440 3.07e-14 73.9

Find 127 sgRNAs with CRISPR-Local

Find 188 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCATATGGTCCTAATTTCT+TGG 0.241465 8_3:+46823821 Msa1291460:CDS
ATTAACACATCTTTAGTAAA+AGG 0.243544 8_3:-46826124 None:intergenic
GAAAGGAGGGTTCTTTAGAT+TGG 0.243763 8_3:+46823700 Msa1291460:CDS
TATGAATCCACCATTGAATT+AGG 0.277485 8_3:-46823804 None:intergenic
TGCAATGGATTAACCTGATT+TGG 0.278397 8_3:-46825393 None:intergenic
AGTGGAGAACCTTCTATTAA+AGG 0.279819 8_3:+46824347 Msa1291460:CDS
GACTAGAACCGTACATCTTT+AGG 0.302334 8_3:-46825096 None:intergenic
AAAATCCACATGTGGATTTA+CGG 0.315802 8_3:-46825055 None:intergenic
CACTTGCACAATCCAACATT+TGG 0.316306 8_3:-46824328 None:intergenic
CCTTCATCTTGAGACATTAC+AGG 0.318475 8_3:-46824239 None:intergenic
AGGGATTAACTCTAGGTTAA+AGG 0.325942 8_3:+46826045 Msa1291460:three_prime_UTR
GGATTTAGACCTTTAATAGA+AGG 0.338159 8_3:-46824356 None:intergenic
TGCAAATTCATCTGTAGGTT+TGG 0.339897 8_3:+46823743 Msa1291460:CDS
AGTCACTTCTTCGATCATTC+TGG 0.342191 8_3:-46824214 None:intergenic
ACCGGGTCCATCATCTGCTC+TGG 0.343461 8_3:+46825212 Msa1291460:CDS
GTAAGAAGGTCCAAGAAATT+AGG 0.353442 8_3:-46823831 None:intergenic
TCAACCACAGTTGGAACCTT+TGG 0.357269 8_3:+46825353 Msa1291460:CDS
TCTGATAAATTTGCCATGTT+AGG 0.358693 8_3:-46825312 None:intergenic
AATACAGCACAATGCCTCTT+TGG 0.360099 8_3:+46825911 Msa1291460:three_prime_UTR
TCTTACTAACTCCGAAAACT+TGG 0.372119 8_3:+46823848 Msa1291460:CDS
TCAGATTCTTCAACATCAAC+CGG 0.372154 8_3:+46825194 Msa1291460:intron
TTCTTCATGTCCTAATTCAA+TGG 0.389007 8_3:+46823794 Msa1291460:CDS
ATCTGTAGGTTTGGTTTCCA+TGG 0.397148 8_3:+46823752 Msa1291460:CDS
TTCCATGGAAAATTCTATGA+TGG 0.411759 8_3:+46823767 Msa1291460:CDS
GGTTGATATGAATGTGATAA+TGG 0.412278 8_3:-46825267 None:intergenic
GTGTGGGATTCCTCAATCTA+TGG 0.414177 8_3:+46824133 Msa1291460:CDS
CTTCTTCGATCATTCTGGAT+TGG 0.414720 8_3:-46824209 None:intergenic
GTTGAGGAAATTTCAGTCCT+TGG 0.419505 8_3:-46825421 None:intergenic
GGAAATATCTCAGCCCCATT+TGG 0.420071 8_3:-46824014 None:intergenic
CAGTCCTTGGCTGCTTGCAA+TGG 0.420935 8_3:-46825408 None:intergenic
GAAAACTGAGACAAACTTTG+AGG 0.438701 8_3:-46824035 None:intergenic
AGGAGGGTTCTTTAGATTGG+AGG 0.438856 8_3:+46823703 Msa1291460:CDS
CCGGGTCCATCATCTGCTCT+GGG 0.439623 8_3:+46825213 Msa1291460:CDS
AGCAATGAAGTGAGAAAAGA+TGG 0.442777 8_3:+46823897 Msa1291460:CDS
ATGGGTTGGGACCTTGGTAG+TGG 0.442811 8_3:+46823963 Msa1291460:CDS
TCTCGGTCTGTTGAGCATCT+TGG 0.450146 8_3:+46824173 Msa1291460:CDS
GTTGGAATGGGTTGGGACCT+TGG 0.452583 8_3:+46823957 Msa1291460:CDS
TAGTATCTCATATATATAAC+CGG 0.453048 8_3:+46825964 Msa1291460:three_prime_UTR
ACTTGCACAATCCAACATTT+GGG 0.456863 8_3:-46824327 None:intergenic
AGGGTTTAAAGAGCCAAATC+AGG 0.463980 8_3:+46825380 Msa1291460:CDS
CAAATTTATCAGATATGCTT+AGG 0.466891 8_3:+46825322 Msa1291460:CDS
TGATCTTCATTCTTCATCAT+CGG 0.474379 8_3:+46825879 Msa1291460:three_prime_UTR
TGTGTTAATCTATGATCTCA+TGG 0.474987 8_3:+46826138 Msa1291460:three_prime_UTR
AATTCAATGGTGGATTCATA+TGG 0.475276 8_3:+46823807 Msa1291460:CDS
GAAGTGAGAAAAGATGGGTT+TGG 0.476292 8_3:+46823903 Msa1291460:CDS
AAGGTCTAAATCCTAAGAAA+AGG 0.480320 8_3:+46824366 Msa1291460:CDS
CTCTACCTCTACCTGTGTCT+CGG 0.483360 8_3:+46824156 Msa1291460:CDS
GGCACACTGAACAAACTTCA+AGG 0.485234 8_3:-46826103 None:intergenic
CCACAGTTGGAACCTTTGGC+TGG 0.487866 8_3:+46825357 Msa1291460:CDS
CATCCAAGTTGTGGCTACTC+AGG 0.488215 8_3:-46825726 None:intergenic
AGTTGGAACCTTTGGCTGGA+GGG 0.488222 8_3:+46825361 Msa1291460:CDS
GTTATATATATGAGATACTA+AGG 0.496943 8_3:-46825961 None:intergenic
TAAATAGTAAATAGAAATGA+TGG 0.498536 8_3:+46825499 Msa1291460:three_prime_UTR
TAGAACAGTTGGAATGGGTT+GGG 0.502266 8_3:+46823950 Msa1291460:CDS
TGATGATAGAACAGTTGGAA+TGG 0.507282 8_3:+46823944 Msa1291460:CDS
TTTGAGGTAGATAGAAAGGA+GGG 0.507780 8_3:+46823687 Msa1291460:CDS
GGTTATGATGATAGAACAGT+TGG 0.507995 8_3:+46823939 Msa1291460:CDS
TCTCCTGAGTAGCCACAACT+TGG 0.508367 8_3:+46825723 Msa1291460:three_prime_UTR
CTCTAGTGTCAAGGCTGTGA+AGG 0.511372 8_3:+46826025 Msa1291460:three_prime_UTR
TCCATGGAAAATTCTATGAT+GGG 0.513525 8_3:+46823768 Msa1291460:CDS
AGGTTAAAGGAAGTAATCAA+TGG 0.515880 8_3:+46826058 Msa1291460:three_prime_UTR
AAAGTTTATTTCTTTCTACT+AGG 0.516977 8_3:+46825585 Msa1291460:three_prime_UTR
CGATGATGGTTCCCAAATGT+TGG 0.518846 8_3:+46824316 Msa1291460:CDS
AGAAGTACTTCATCCACAAA+AGG 0.520131 8_3:-46825994 None:intergenic
CCTGTAATGTCTCAAGATGA+AGG 0.520207 8_3:+46824239 Msa1291460:CDS
AGAAACCCCAGAGCAGATGA+TGG 0.522386 8_3:-46825219 None:intergenic
GATGAAGGGAAACAAGCTCT+TGG 0.524819 8_3:+46824254 Msa1291460:CDS
TTAACTTTCATCTGACCAGC+TGG 0.527755 8_3:-46825852 None:intergenic
AACCTTGCAAATTCATCTGT+AGG 0.531570 8_3:+46823738 Msa1291460:CDS
CTCAGGAGAAGTTGTTGTGA+AGG 0.532326 8_3:-46825709 None:intergenic
GCAGACTGTTCAACCACAGT+TGG 0.533598 8_3:+46825344 Msa1291460:CDS
GATGATAGAACAGTTGGAAT+GGG 0.534254 8_3:+46823945 Msa1291460:CDS
ATAGAACAGTTGGAATGGGT+TGG 0.546913 8_3:+46823949 Msa1291460:CDS
GAAGGGAAACAAGCTCTTGG+AGG 0.547800 8_3:+46824257 Msa1291460:CDS
TAATCCATTGCAAGCAGCCA+AGG 0.549245 8_3:+46825404 Msa1291460:CDS
GTTGAGCATCTTGGTAGTGA+TGG 0.549328 8_3:+46824182 Msa1291460:CDS
ACAGACCGAGACACAGGTAG+AGG 0.549978 8_3:-46824161 None:intergenic
TCTTTAAACCCTCCAGCCAA+AGG 0.552371 8_3:-46825369 None:intergenic
CCCAGAGCAGATGATGGACC+CGG 0.555611 8_3:-46825213 None:intergenic
TTCATGTCCTAATTCAATGG+TGG 0.556761 8_3:+46823797 Msa1291460:CDS
CAGTTGGAACCTTTGGCTGG+AGG 0.557432 8_3:+46825360 Msa1291460:CDS
TTAGTAATTCTCTAGTGTCA+AGG 0.558599 8_3:+46826016 Msa1291460:three_prime_UTR
AGAGGTAGAGCCATAGATTG+AGG 0.558788 8_3:-46824143 None:intergenic
CTGAGTAGCCACAACTTGGA+TGG 0.560886 8_3:+46825727 Msa1291460:three_prime_UTR
CATCATATCCACGAAACTCC+CGG 0.562237 8_3:-46824105 None:intergenic
GATGAATCGAGTGGCGATGA+TGG 0.566803 8_3:+46824302 Msa1291460:CDS
ATCCACAAAAGGTATTAGAC+CGG 0.568752 8_3:-46825983 None:intergenic
GCTGTGAAGGGATTAACTCT+AGG 0.570898 8_3:+46826038 Msa1291460:three_prime_UTR
CTGTAATGTCTCAAGATGAA+GGG 0.576780 8_3:+46824240 Msa1291460:CDS
AGAGTTGAATGAATCAGTCC+TGG 0.580047 8_3:-46825288 None:intergenic
CTTACTAACTCCGAAAACTT+GGG 0.581398 8_3:+46823849 Msa1291460:CDS
CAAATGTTGGATTGTGCAAG+TGG 0.582909 8_3:+46824329 Msa1291460:CDS
ACTAACTCCGAAAACTTGGG+TGG 0.585659 8_3:+46823852 Msa1291460:CDS
GGGTCTTGTAACGAAGCTGA+TGG 0.585899 8_3:+46824278 Msa1291460:CDS
AACCTACAGATGAATTTGCA+AGG 0.591369 8_3:-46823740 None:intergenic
TAGTAAATAGAAATGATGGT+TGG 0.593178 8_3:+46825503 Msa1291460:three_prime_UTR
AAGGGAAACAAGCTCTTGGA+GGG 0.596219 8_3:+46824258 Msa1291460:CDS
TCTAGTGTCAAGGCTGTGAA+GGG 0.596816 8_3:+46826026 Msa1291460:three_prime_UTR
GTAGTGGTTCTATGATGAAG+AGG 0.598345 8_3:+46823979 Msa1291460:CDS
GATGCAGGTATCGTGCCAGC+TGG 0.600320 8_3:+46825837 Msa1291460:intron
GTTGTTGTGAAGGTTGTTTG+TGG 0.601204 8_3:-46825699 None:intergenic
TTAGATTGGAGGTTTGTGAG+TGG 0.604373 8_3:+46823714 Msa1291460:CDS
CTGGATTTATTGATGCTGCA+AGG 0.604609 8_3:+46824066 Msa1291460:CDS
ACATCTTACCATCCAAGTTG+TGG 0.609036 8_3:-46825735 None:intergenic
TCCATATTAAAATCCACATG+TGG 0.609814 8_3:-46825063 None:intergenic
ATGATGAAGAATGAAGATCA+AGG 0.610173 8_3:-46825876 None:intergenic
GATAATGGAGGAAAAGCCAT+TGG 0.610652 8_3:-46825252 None:intergenic
TCATTCAACTCTACCTAACA+TGG 0.611728 8_3:+46825299 Msa1291460:CDS
TGCTCAACAGACCGAGACAC+AGG 0.613918 8_3:-46824167 None:intergenic
CAGATTCTTCAACATCAACC+GGG 0.618225 8_3:+46825195 Msa1291460:intron
GAATCCAGAAAATACCAAAG+AGG 0.621606 8_3:-46825925 None:intergenic
CCAGCCAAAGGTTCCAACTG+TGG 0.623573 8_3:-46825357 None:intergenic
TAGTGGTTCTATGATGAAGA+GGG 0.626282 8_3:+46823980 Msa1291460:CDS
TGGATATGATGAACTCGTGT+GGG 0.632999 8_3:+46824117 Msa1291460:CDS
TGCAACCGTAAATCCACATG+TGG 0.638726 8_3:+46825050 Msa1291460:CDS
GCACACTGAACAAACTTCAA+GGG 0.642076 8_3:-46826102 None:intergenic
TGATATGAATGTGATAATGG+AGG 0.657822 8_3:-46825264 None:intergenic
GCAATGAAGTGAGAAAAGAT+GGG 0.658069 8_3:+46823898 Msa1291460:CDS
GGTTCTATGATGAAGAGGGA+TGG 0.658101 8_3:+46823984 Msa1291460:CDS
CGGGTCCATCATCTGCTCTG+GGG 0.660359 8_3:+46825214 Msa1291460:CDS
GTTTCTCAGAGAAATGCCAA+TGG 0.663274 8_3:+46825236 Msa1291460:CDS
GTGGATATGATGAACTCGTG+TGG 0.663347 8_3:+46824116 Msa1291460:CDS
AATTTAACACTGAACAAACA+AGG 0.676173 8_3:-46826198 None:intergenic
GCTGATGGAGATGAATCGAG+TGG 0.686428 8_3:+46824293 Msa1291460:CDS
TCATCATAGAACCACTACCA+AGG 0.687049 8_3:-46823974 None:intergenic
TTATCACATTCATATCAACC+AGG 0.710399 8_3:+46825270 Msa1291460:CDS
ATAGCTGCATGAAGTGTGCG+AGG 0.713396 8_3:+46825659 Msa1291460:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAAAAAAATTCATATTTTGT+TGG - chr8_3:46824513-46824532 None:intergenic 10.0%
!!! ATTTCTAGAAACATATATAA+GGG - chr8_3:46824963-46824982 None:intergenic 15.0%
!!! GACAATATTCAATTTTATAA+AGG - chr8_3:46825176-46825195 None:intergenic 15.0%
!! TGTAAAACTGAAATTAAAAA+AGG - chr8_3:46825453-46825472 None:intergenic 15.0%
!! TAAATAGTAAATAGAAATGA+TGG + chr8_3:46825499-46825518 Msa1291460:three_prime_UTR 15.0%
!! AAAATTCATCATAGTGAATT+GGG + chr8_3:46824673-46824692 Msa1291460:intron 20.0%
!! AAATTATTGCAATTTCAAAC+AGG - chr8_3:46824830-46824849 None:intergenic 20.0%
!!! GATTTCTAGAAACATATATA+AGG - chr8_3:46824964-46824983 None:intergenic 20.0%
!! AACTGAAATTAAAAAAGGAT+TGG - chr8_3:46825448-46825467 None:intergenic 20.0%
!!! AAAGTTTATTTCTTTCTACT+AGG + chr8_3:46825585-46825604 Msa1291460:three_prime_UTR 20.0%
!! ATTTAGCAAGAAATATTAGA+AGG - chr8_3:46825775-46825794 None:intergenic 20.0%
!! GTTATATATATGAGATACTA+AGG - chr8_3:46825964-46825983 None:intergenic 20.0%
!! TAGTATCTCATATATATAAC+CGG + chr8_3:46825964-46825983 Msa1291460:three_prime_UTR 20.0%
!! ATTAACACATCTTTAGTAAA+AGG - chr8_3:46826127-46826146 None:intergenic 20.0%
!!! AAATTTTGAGGTAGATAGAA+AGG + chr8_3:46823683-46823702 Msa1291460:CDS 25.0%
!!! CCATTTTTTTTCCTTTTCTT+AGG - chr8_3:46824380-46824399 None:intergenic 25.0%
! CCTAAGAAAAGGAAAAAAAA+TGG + chr8_3:46824377-46824396 Msa1291460:CDS 25.0%
!!! TTTTAGTATAGTTGATACTG+TGG + chr8_3:46824423-46824442 Msa1291460:intron 25.0%
!! TTGTCATTTGATTAATGACT+TGG - chr8_3:46824598-46824617 None:intergenic 25.0%
! GTCATTAATCAAATGACAAA+AGG + chr8_3:46824599-46824618 Msa1291460:intron 25.0%
!! ACTAGAGCTATACTAATTTT+AGG + chr8_3:46824628-46824647 Msa1291460:intron 25.0%
! CAAAATTCATCATAGTGAAT+TGG + chr8_3:46824672-46824691 Msa1291460:intron 25.0%
! ATCTTTGTTACAATTGGTAA+AGG + chr8_3:46824885-46824904 Msa1291460:intron 25.0%
!!! ATACGATAAACTGTTTTGTT+AGG + chr8_3:46824921-46824940 Msa1291460:intron 25.0%
!!! ATGTGGATTTTAATATGGAA+CGG + chr8_3:46825067-46825086 Msa1291460:CDS 25.0%
!!! ATTGTAAGCTTTAAAATTGC+TGG - chr8_3:46825132-46825151 None:intergenic 25.0%
! CAAATTTATCAGATATGCTT+AGG + chr8_3:46825322-46825341 Msa1291460:CDS 25.0%
! TAGTAAATAGAAATGATGGT+TGG + chr8_3:46825503-46825522 Msa1291460:three_prime_UTR 25.0%
! AATTTAACACTGAACAAACA+AGG - chr8_3:46826201-46826220 None:intergenic 25.0%
! GAGTGAAAGTGAAAATTTTG+AGG + chr8_3:46823671-46823690 Msa1291460:CDS 30.0%
TTCCATGGAAAATTCTATGA+TGG + chr8_3:46823767-46823786 Msa1291460:CDS 30.0%
TCCATGGAAAATTCTATGAT+GGG + chr8_3:46823768-46823787 Msa1291460:CDS 30.0%
TTCTTCATGTCCTAATTCAA+TGG + chr8_3:46823794-46823813 Msa1291460:CDS 30.0%
TATGAATCCACCATTGAATT+AGG - chr8_3:46823807-46823826 None:intergenic 30.0%
!! AATTCAATGGTGGATTCATA+TGG + chr8_3:46823807-46823826 Msa1291460:CDS 30.0%
TTCATATGGTCCTAATTTCT+TGG + chr8_3:46823821-46823840 Msa1291460:CDS 30.0%
GGATTTAGACCTTTAATAGA+AGG - chr8_3:46824359-46824378 None:intergenic 30.0%
AAGGTCTAAATCCTAAGAAA+AGG + chr8_3:46824366-46824385 Msa1291460:CDS 30.0%
GAAAAGGAAAAAAAATGGAC+AGG + chr8_3:46824382-46824401 Msa1291460:CDS 30.0%
ATACTGTGGTTAACAATTTG+AGG + chr8_3:46824437-46824456 Msa1291460:intron 30.0%
!! CAGCTTATAATTTTGCTATG+TGG - chr8_3:46824548-46824567 None:intergenic 30.0%
TTGGAACTATGACTATATTG+AGG - chr8_3:46824579-46824598 None:intergenic 30.0%
AATTGTAACAAAGATGACAC+TGG - chr8_3:46824880-46824899 None:intergenic 30.0%
AGTGTCATCTTTGTTACAAT+TGG + chr8_3:46824879-46824898 Msa1291460:intron 30.0%
ATATAAGGGACAACTAACAA+AGG - chr8_3:46824949-46824968 None:intergenic 30.0%
ATTGATAAAAACTGCCAAGA+GGG - chr8_3:46825024-46825043 None:intergenic 30.0%
AAAATCCACATGTGGATTTA+CGG - chr8_3:46825058-46825077 None:intergenic 30.0%
!! TCCACATGTGGATTTTAATA+TGG + chr8_3:46825062-46825081 Msa1291460:CDS 30.0%
TCCATATTAAAATCCACATG+TGG - chr8_3:46825066-46825085 None:intergenic 30.0%
! CAATTTTATAAAGGTCAGTC+AGG - chr8_3:46825167-46825186 None:intergenic 30.0%
!! TGATATGAATGTGATAATGG+AGG - chr8_3:46825267-46825286 None:intergenic 30.0%
! GGTTGATATGAATGTGATAA+TGG - chr8_3:46825270-46825289 None:intergenic 30.0%
TTATCACATTCATATCAACC+AGG + chr8_3:46825270-46825289 Msa1291460:CDS 30.0%
TCTGATAAATTTGCCATGTT+AGG - chr8_3:46825315-46825334 None:intergenic 30.0%
!! TTAAAAAAGGATTGGTGTTG+AGG - chr8_3:46825440-46825459 None:intergenic 30.0%
ATGATGAAGAATGAAGATCA+AGG - chr8_3:46825879-46825898 None:intergenic 30.0%
TGATCTTCATTCTTCATCAT+CGG + chr8_3:46825879-46825898 Msa1291460:three_prime_UTR 30.0%
TTAGTAATTCTCTAGTGTCA+AGG + chr8_3:46826016-46826035 Msa1291460:three_prime_UTR 30.0%
AGGTTAAAGGAAGTAATCAA+TGG + chr8_3:46826058-46826077 Msa1291460:three_prime_UTR 30.0%
TGTGTTAATCTATGATCTCA+TGG + chr8_3:46826138-46826157 Msa1291460:three_prime_UTR 30.0%
!!! TTTTGAGGTAGATAGAAAGG+AGG + chr8_3:46823686-46823705 Msa1291460:CDS 35.0%
! TTTGAGGTAGATAGAAAGGA+GGG + chr8_3:46823687-46823706 Msa1291460:CDS 35.0%
AACCTTGCAAATTCATCTGT+AGG + chr8_3:46823738-46823757 Msa1291460:CDS 35.0%
AACCTACAGATGAATTTGCA+AGG - chr8_3:46823743-46823762 None:intergenic 35.0%
TGCAAATTCATCTGTAGGTT+TGG + chr8_3:46823743-46823762 Msa1291460:CDS 35.0%
! ACCCATCATAGAATTTTCCA+TGG - chr8_3:46823772-46823791 None:intergenic 35.0%
TTCATGTCCTAATTCAATGG+TGG + chr8_3:46823797-46823816 Msa1291460:CDS 35.0%
GTAAGAAGGTCCAAGAAATT+AGG - chr8_3:46823834-46823853 None:intergenic 35.0%
! AGTTTTCGGAGTTAGTAAGA+AGG - chr8_3:46823848-46823867 None:intergenic 35.0%
TCTTACTAACTCCGAAAACT+TGG + chr8_3:46823848-46823867 Msa1291460:CDS 35.0%
CTTACTAACTCCGAAAACTT+GGG + chr8_3:46823849-46823868 Msa1291460:CDS 35.0%
! ACAAAAACCACCCAAGTTTT+CGG - chr8_3:46823862-46823881 None:intergenic 35.0%
! GGTGGTTTTTGTGATGTTAA+TGG + chr8_3:46823870-46823889 Msa1291460:CDS 35.0%
AGCAATGAAGTGAGAAAAGA+TGG + chr8_3:46823897-46823916 Msa1291460:CDS 35.0%
GCAATGAAGTGAGAAAAGAT+GGG + chr8_3:46823898-46823917 Msa1291460:CDS 35.0%
GGTTATGATGATAGAACAGT+TGG + chr8_3:46823939-46823958 Msa1291460:CDS 35.0%
TGATGATAGAACAGTTGGAA+TGG + chr8_3:46823944-46823963 Msa1291460:CDS 35.0%
GATGATAGAACAGTTGGAAT+GGG + chr8_3:46823945-46823964 Msa1291460:CDS 35.0%
! TAGTGGTTCTATGATGAAGA+GGG + chr8_3:46823980-46823999 Msa1291460:CDS 35.0%
GAAAACTGAGACAAACTTTG+AGG - chr8_3:46824038-46824057 None:intergenic 35.0%
!! TCTCAGTTTTCAACTGATTC+TGG + chr8_3:46824047-46824066 Msa1291460:CDS 35.0%
CTGTAATGTCTCAAGATGAA+GGG + chr8_3:46824240-46824259 Msa1291460:CDS 35.0%
ACTTGCACAATCCAACATTT+GGG - chr8_3:46824330-46824349 None:intergenic 35.0%
AGTGGAGAACCTTCTATTAA+AGG + chr8_3:46824347-46824366 Msa1291460:CDS 35.0%
TTGAGGTTCAAATCGTGAAT+TGG + chr8_3:46824454-46824473 Msa1291460:intron 35.0%
CATTGATAAAAACTGCCAAG+AGG - chr8_3:46825025-46825044 None:intergenic 35.0%
TCAGATTCTTCAACATCAAC+CGG + chr8_3:46825194-46825213 Msa1291460:intron 35.0%
TCATTCAACTCTACCTAACA+TGG + chr8_3:46825299-46825318 Msa1291460:CDS 35.0%
!! TGCAATGGATTAACCTGATT+TGG - chr8_3:46825396-46825415 None:intergenic 35.0%
! AATGCCTCTTTGGTATTTTC+TGG + chr8_3:46825921-46825940 Msa1291460:three_prime_UTR 35.0%
GAATCCAGAAAATACCAAAG+AGG - chr8_3:46825928-46825947 None:intergenic 35.0%
!! ATCCACAAAAGGTATTAGAC+CGG - chr8_3:46825986-46826005 None:intergenic 35.0%
!! AGAAGTACTTCATCCACAAA+AGG - chr8_3:46825997-46826016 None:intergenic 35.0%
AGGGATTAACTCTAGGTTAA+AGG + chr8_3:46826045-46826064 Msa1291460:three_prime_UTR 35.0%
GAAAGGAGGGTTCTTTAGAT+TGG + chr8_3:46823700-46823719 Msa1291460:CDS 40.0%
TTAGATTGGAGGTTTGTGAG+TGG + chr8_3:46823714-46823733 Msa1291460:CDS 40.0%
! ATCTGTAGGTTTGGTTTCCA+TGG + chr8_3:46823752-46823771 Msa1291460:CDS 40.0%
! GAAGTGAGAAAAGATGGGTT+TGG + chr8_3:46823903-46823922 Msa1291460:CDS 40.0%
!!! AAGATGGGTTTGGTTTTGCA+AGG + chr8_3:46823913-46823932 Msa1291460:CDS 40.0%
!!! AGATGGGTTTGGTTTTGCAA+GGG + chr8_3:46823914-46823933 Msa1291460:CDS 40.0%
ATAGAACAGTTGGAATGGGT+TGG + chr8_3:46823949-46823968 Msa1291460:CDS 40.0%
! TAGAACAGTTGGAATGGGTT+GGG + chr8_3:46823950-46823969 Msa1291460:CDS 40.0%
TCATCATAGAACCACTACCA+AGG - chr8_3:46823977-46823996 None:intergenic 40.0%
! GTAGTGGTTCTATGATGAAG+AGG + chr8_3:46823979-46823998 Msa1291460:CDS 40.0%
! AGGGATGGTGTTTTACCAAA+TGG + chr8_3:46823999-46824018 Msa1291460:CDS 40.0%
! GGGATGGTGTTTTACCAAAT+GGG + chr8_3:46824000-46824019 Msa1291460:CDS 40.0%
! GGATGGTGTTTTACCAAATG+GGG + chr8_3:46824001-46824020 Msa1291460:CDS 40.0%
CTGGATTTATTGATGCTGCA+AGG + chr8_3:46824066-46824085 Msa1291460:CDS 40.0%
!!! AGGATGTCATGTTTTAGTGC+CGG + chr8_3:46824086-46824105 Msa1291460:CDS 40.0%
TGGATATGATGAACTCGTGT+GGG + chr8_3:46824117-46824136 Msa1291460:CDS 40.0%
CTTCTTCGATCATTCTGGAT+TGG - chr8_3:46824212-46824231 None:intergenic 40.0%
AGTCACTTCTTCGATCATTC+TGG - chr8_3:46824217-46824236 None:intergenic 40.0%
CCTTCATCTTGAGACATTAC+AGG - chr8_3:46824242-46824261 None:intergenic 40.0%
CCTGTAATGTCTCAAGATGA+AGG + chr8_3:46824239-46824258 Msa1291460:CDS 40.0%
CACTTGCACAATCCAACATT+TGG - chr8_3:46824331-46824350 None:intergenic 40.0%
CAAATGTTGGATTGTGCAAG+TGG + chr8_3:46824329-46824348 Msa1291460:CDS 40.0%
! TGACACATTTTTCTCCCTCT+TGG + chr8_3:46825007-46825026 Msa1291460:intron 40.0%
GACTAGAACCGTACATCTTT+AGG - chr8_3:46825099-46825118 None:intergenic 40.0%
CAGATTCTTCAACATCAACC+GGG + chr8_3:46825195-46825214 Msa1291460:intron 40.0%
GTTTCTCAGAGAAATGCCAA+TGG + chr8_3:46825236-46825255 Msa1291460:CDS 40.0%
!! GATAATGGAGGAAAAGCCAT+TGG - chr8_3:46825255-46825274 None:intergenic 40.0%
AGAGTTGAATGAATCAGTCC+TGG - chr8_3:46825291-46825310 None:intergenic 40.0%
AGGGTTTAAAGAGCCAAATC+AGG + chr8_3:46825380-46825399 Msa1291460:CDS 40.0%
GTTGAGGAAATTTCAGTCCT+TGG - chr8_3:46825424-46825443 None:intergenic 40.0%
GTTGTTGTGAAGGTTGTTTG+TGG - chr8_3:46825702-46825721 None:intergenic 40.0%
ACATCTTACCATCCAAGTTG+TGG - chr8_3:46825738-46825757 None:intergenic 40.0%
! TGCTAAATTTTGCCAGAGAG+AGG + chr8_3:46825787-46825806 Msa1291460:intron 40.0%
TTAACTTTCATCTGACCAGC+TGG - chr8_3:46825855-46825874 None:intergenic 40.0%
AATACAGCACAATGCCTCTT+TGG + chr8_3:46825911-46825930 Msa1291460:three_prime_UTR 40.0%
! AACCGGTCTAATACCTTTTG+TGG + chr8_3:46825981-46826000 Msa1291460:three_prime_UTR 40.0%
GCACACTGAACAAACTTCAA+GGG - chr8_3:46826105-46826124 None:intergenic 40.0%
AGGAGGGTTCTTTAGATTGG+AGG + chr8_3:46823703-46823722 Msa1291460:CDS 45.0%
ACTAACTCCGAAAACTTGGG+TGG + chr8_3:46823852-46823871 Msa1291460:CDS 45.0%
GGTTCTATGATGAAGAGGGA+TGG + chr8_3:46823984-46824003 Msa1291460:CDS 45.0%
GGAAATATCTCAGCCCCATT+TGG - chr8_3:46824017-46824036 None:intergenic 45.0%
!!! GGATGTCATGTTTTAGTGCC+GGG + chr8_3:46824087-46824106 Msa1291460:CDS 45.0%
CATCATATCCACGAAACTCC+CGG - chr8_3:46824108-46824127 None:intergenic 45.0%
GTGGATATGATGAACTCGTG+TGG + chr8_3:46824116-46824135 Msa1291460:CDS 45.0%
GTGTGGGATTCCTCAATCTA+TGG + chr8_3:46824133-46824152 Msa1291460:CDS 45.0%
AGAGGTAGAGCCATAGATTG+AGG - chr8_3:46824146-46824165 None:intergenic 45.0%
!! GTTGAGCATCTTGGTAGTGA+TGG + chr8_3:46824182-46824201 Msa1291460:CDS 45.0%
GATGAAGGGAAACAAGCTCT+TGG + chr8_3:46824254-46824273 Msa1291460:CDS 45.0%
AAGGGAAACAAGCTCTTGGA+GGG + chr8_3:46824258-46824277 Msa1291460:CDS 45.0%
! CGATGATGGTTCCCAAATGT+TGG + chr8_3:46824316-46824335 Msa1291460:CDS 45.0%
TGCAACCGTAAATCCACATG+TGG + chr8_3:46825050-46825069 Msa1291460:CDS 45.0%
GGCTGCTTCCTAAAGATGTA+CGG + chr8_3:46825088-46825107 Msa1291460:intron 45.0%
TCAACCACAGTTGGAACCTT+TGG + chr8_3:46825353-46825372 Msa1291460:CDS 45.0%
TCTTTAAACCCTCCAGCCAA+AGG - chr8_3:46825372-46825391 None:intergenic 45.0%
TAATCCATTGCAAGCAGCCA+AGG + chr8_3:46825404-46825423 Msa1291460:CDS 45.0%
CTCAGGAGAAGTTGTTGTGA+AGG - chr8_3:46825712-46825731 None:intergenic 45.0%
! ATTTTGCCAGAGAGAGGTAG+TGG + chr8_3:46825793-46825812 Msa1291460:intron 45.0%
TCTAGTGTCAAGGCTGTGAA+GGG + chr8_3:46826026-46826045 Msa1291460:three_prime_UTR 45.0%
GCTGTGAAGGGATTAACTCT+AGG + chr8_3:46826038-46826057 Msa1291460:three_prime_UTR 45.0%
GGCACACTGAACAAACTTCA+AGG - chr8_3:46826106-46826125 None:intergenic 45.0%
!!! GGGTTTGGTTTTGCAAGGGT+TGG + chr8_3:46823918-46823937 Msa1291460:CDS 50.0%
!! TTTTAGTGCCGGGAGTTTCG+TGG + chr8_3:46824097-46824116 Msa1291460:CDS 50.0%
CTCTACCTCTACCTGTGTCT+CGG + chr8_3:46824156-46824175 Msa1291460:CDS 50.0%
TCTCGGTCTGTTGAGCATCT+TGG + chr8_3:46824173-46824192 Msa1291460:CDS 50.0%
GAAGGGAAACAAGCTCTTGG+AGG + chr8_3:46824257-46824276 Msa1291460:CDS 50.0%
! GGGTCTTGTAACGAAGCTGA+TGG + chr8_3:46824278-46824297 Msa1291460:CDS 50.0%
GCTGATGGAGATGAATCGAG+TGG + chr8_3:46824293-46824312 Msa1291460:CDS 50.0%
GATGAATCGAGTGGCGATGA+TGG + chr8_3:46824302-46824321 Msa1291460:CDS 50.0%
AGAAACCCCAGAGCAGATGA+TGG - chr8_3:46825222-46825241 None:intergenic 50.0%
GCAGACTGTTCAACCACAGT+TGG + chr8_3:46825344-46825363 Msa1291460:CDS 50.0%
AGTTGGAACCTTTGGCTGGA+GGG + chr8_3:46825361-46825380 Msa1291460:CDS 50.0%
ATAGCTGCATGAAGTGTGCG+AGG + chr8_3:46825659-46825678 Msa1291460:three_prime_UTR 50.0%
TCTCCTGAGTAGCCACAACT+TGG + chr8_3:46825723-46825742 Msa1291460:three_prime_UTR 50.0%
CATCCAAGTTGTGGCTACTC+AGG - chr8_3:46825729-46825748 None:intergenic 50.0%
CTGAGTAGCCACAACTTGGA+TGG + chr8_3:46825727-46825746 Msa1291460:three_prime_UTR 50.0%
ACCTGCATCAGCATGGAACA+GGG - chr8_3:46825826-46825845 None:intergenic 50.0%
TACCTGCATCAGCATGGAAC+AGG - chr8_3:46825827-46825846 None:intergenic 50.0%
CTCTAGTGTCAAGGCTGTGA+AGG + chr8_3:46826025-46826044 Msa1291460:three_prime_UTR 50.0%
!! GTTGGAATGGGTTGGGACCT+TGG + chr8_3:46823957-46823976 Msa1291460:CDS 55.0%
!! ATGGGTTGGGACCTTGGTAG+TGG + chr8_3:46823963-46823982 Msa1291460:CDS 55.0%
ACAGACCGAGACACAGGTAG+AGG - chr8_3:46824164-46824183 None:intergenic 55.0%
TGCTCAACAGACCGAGACAC+AGG - chr8_3:46824170-46824189 None:intergenic 55.0%
CCAGCCAAAGGTTCCAACTG+TGG - chr8_3:46825360-46825379 None:intergenic 55.0%
CCACAGTTGGAACCTTTGGC+TGG + chr8_3:46825357-46825376 Msa1291460:CDS 55.0%
CAGTTGGAACCTTTGGCTGG+AGG + chr8_3:46825360-46825379 Msa1291460:CDS 55.0%
CAGTCCTTGGCTGCTTGCAA+TGG - chr8_3:46825411-46825430 None:intergenic 55.0%
AGCAGTCCACTACCTCTCTC+TGG - chr8_3:46825802-46825821 None:intergenic 55.0%
GCACGATACCTGCATCAGCA+TGG - chr8_3:46825833-46825852 None:intergenic 55.0%
ACCGGGTCCATCATCTGCTC+TGG + chr8_3:46825212-46825231 Msa1291460:CDS 60.0%
!! CCCAGAGCAGATGATGGACC+CGG - chr8_3:46825216-46825235 None:intergenic 60.0%
CCGGGTCCATCATCTGCTCT+GGG + chr8_3:46825213-46825232 Msa1291460:CDS 60.0%
CGGGTCCATCATCTGCTCTG+GGG + chr8_3:46825214-46825233 Msa1291460:CDS 60.0%
GCCCTGTTCCATGCTGATGC+AGG + chr8_3:46825822-46825841 Msa1291460:intron 60.0%
! GATGCAGGTATCGTGCCAGC+TGG + chr8_3:46825837-46825856 Msa1291460:intron 60.0%
Chromosome Type Strat End Strand Name
chr8_3 gene 46823669 46826236 46823669 ID=Msa1291460;Name=Msa1291460
chr8_3 mRNA 46823669 46826236 46823669 ID=Msa1291460-mRNA-1;Parent=Msa1291460;Name=Msa1291460-mRNA-1;_AED=0.03;_eAED=0.03;_QI=0|0.33|0.25|1|0.66|0.5|4|710|346
chr8_3 exon 46823669 46824403 46823669 ID=Msa1291460-mRNA-1:exon:13003;Parent=Msa1291460-mRNA-1
chr8_3 exon 46825033 46825104 46825033 ID=Msa1291460-mRNA-1:exon:13004;Parent=Msa1291460-mRNA-1
chr8_3 exon 46825198 46825748 46825198 ID=Msa1291460-mRNA-1:exon:13005;Parent=Msa1291460-mRNA-1
chr8_3 exon 46825844 46826236 46825844 ID=Msa1291460-mRNA-1:exon:13006;Parent=Msa1291460-mRNA-1
chr8_3 CDS 46823669 46824403 46823669 ID=Msa1291460-mRNA-1:cds;Parent=Msa1291460-mRNA-1
chr8_3 CDS 46825033 46825104 46825033 ID=Msa1291460-mRNA-1:cds;Parent=Msa1291460-mRNA-1
chr8_3 CDS 46825198 46825431 46825198 ID=Msa1291460-mRNA-1:cds;Parent=Msa1291460-mRNA-1
chr8_3 three_prime_UTR 46825432 46825748 46825432 ID=Msa1291460-mRNA-1:three_prime_utr;Parent=Msa1291460-mRNA-1
chr8_3 three_prime_UTR 46825844 46826236 46825844 ID=Msa1291460-mRNA-1:three_prime_utr;Parent=Msa1291460-mRNA-1
Gene Sequence

>Msa1291460

ATGAGTGAAAGTGAAAATTTTGAGGTAGATAGAAAGGAGGGTTCTTTAGATTGGAGGTTTGTGAGTGGAAACCTTGCAAATTCATCTGTAGGTTTGGTTTCCATGGAAAATTCTATGATGGGTTCTTCTTCATGTCCTAATTCAATGGTGGATTCATATGGTCCTAATTTCTTGGACCTTCTTACTAACTCCGAAAACTTGGGTGGTTTTTGTGATGTTAATGGTCAGAGCAATGAAGTGAGAAAAGATGGGTTTGGTTTTGCAAGGGTTGGTTATGATGATAGAACAGTTGGAATGGGTTGGGACCTTGGTAGTGGTTCTATGATGAAGAGGGATGGTGTTTTACCAAATGGGGCTGAGATATTTCCTCAAAGTTTGTCTCAGTTTTCAACTGATTCTGGATTTATTGATGCTGCAAGGATGTCATGTTTTAGTGCCGGGAGTTTCGTGGATATGATGAACTCGTGTGGGATTCCTCAATCTATGGCTCTACCTCTACCTGTGTCTCGGTCTGTTGAGCATCTTGGTAGTGATGGAAGTCCAATCCAGAATGATCGAAGAAGTGACTGTCCTGTAATGTCTCAAGATGAAGGGAAACAAGCTCTTGGAGGGTCTTGTAACGAAGCTGATGGAGATGAATCGAGTGGCGATGATGGTTCCCAAATGTTGGATTGTGCAAGTGGAGAACCTTCTATTAAAGGTCTAAATCCTAAGAAAAGGAAAAAAAATGGACAGTTTTTATCAATGAAACTTGCAACCGTAAATCCACATGTGGATTTTAATATGGAACGGCTGCTTCCTAAAGATATTCTTCAACATCAACCGGGTCCATCATCTGCTCTGGGGTTTCTCAGAGAAATGCCAATGGCTTTTCCTCCATTATCACATTCATATCAACCAGGACTGATTCATTCAACTCTACCTAACATGGCAAATTTATCAGATATGCTTAGGCAGACTGTTCAACCACAGTTGGAACCTTTGGCTGGAGGGTTTAAAGAGCCAAATCAGGTTAATCCATTGCAAGCAGCCAAGGACTGA
Protein sequence

>Msa1291460

MSESENFEVDRKEGSLDWRFVSGNLANSSVGLVSMENSMMGSSSCPNSMVDSYGPNFLDLLTNSENLGGFCDVNGQSNEVRKDGFGFARVGYDDRTVGMGWDLGSGSMMKRDGVLPNGAEIFPQSLSQFSTDSGFIDAARMSCFSAGSFVDMMNSCGIPQSMALPLPVSRSVEHLGSDGSPIQNDRRSDCPVMSQDEGKQALGGSCNEADGDESSGDDGSQMLDCASGEPSIKGLNPKKRKKNGQFLSMKLATVNPHVDFNMERLLPKDILQHQPGPSSALGFLREMPMAFPPLSHSYQPGLIHSTLPNMANLSDMLRQTVQPQLEPLAGGFKEPNQVNPLQAAKD*