AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1357240


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1357240 MtrunA17_Chr3g0123651 96.311 244 3 2 1 240 3 244 7.62e-173 475
Msa1357240 MtrunA17_Chr8g0381701 65.532 235 60 6 12 239 1 221 1.07e-96 281
Msa1357240 MtrunA17_Chr7g0240291 47.239 163 80 3 81 240 65 224 6.53e-41 140
Msa1357240 MtrunA17_Chr8g0353821 41.618 173 94 4 70 238 77 246 1.71e-40 139
Msa1357240 MtrunA17_Chr8g0353811 43.210 162 85 4 81 238 98 256 6.98e-39 135
Msa1357240 MtrunA17_Chr3g0133611 42.286 175 88 6 75 239 65 236 1.11e-37 132
Msa1357240 MtrunA17_Chr3g0129191 40.719 167 91 5 77 238 95 258 3.99e-37 130
Msa1357240 MtrunA17_Chr4g0062281 37.126 167 91 4 81 238 98 259 9.12e-34 122
Msa1357240 MtrunA17_Chr2g0307001 38.547 179 92 6 70 238 103 273 1.23e-33 122
Msa1357240 MtrunA17_Chr1g0173171 42.188 192 91 5 64 238 95 283 1.93e-32 119
Msa1357240 MtrunA17_Chr2g0307541 36.207 174 102 4 71 238 92 262 4.74e-32 117
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1357240 AT5G65340.1 54.959 242 93 6 9 239 14 250 1.33e-82 247
Msa1357240 AT2G22460.1 58.442 231 85 5 16 239 17 243 1.90e-78 236
Msa1357240 AT1G21050.1 48.035 229 105 6 17 239 25 245 4.38e-60 190
Msa1357240 AT1G21050.2 48.035 229 105 6 17 239 61 281 1.03e-59 190
Msa1357240 AT1G76610.1 44.643 224 105 5 17 239 21 226 2.31e-51 167
Msa1357240 AT2G21990.1 44.253 174 90 4 69 238 81 251 3.91e-43 146
Msa1357240 AT4G39610.1 43.956 182 84 6 69 238 88 263 1.55e-42 145
Msa1357240 AT2G37880.1 44.970 169 83 4 79 239 79 245 3.50e-41 141
Msa1357240 AT5G06990.1 41.618 173 85 4 79 238 91 260 1.28e-38 135
Msa1357240 AT2G41660.1 41.520 171 84 5 81 238 129 296 7.74e-35 126
Msa1357240 AT5G42680.2 41.765 170 91 6 75 239 71 237 9.98e-35 124
Msa1357240 AT5G42680.1 41.765 170 91 6 75 239 71 237 9.98e-35 124
Msa1357240 AT3G25640.1 35.260 173 93 6 81 238 98 266 8.48e-30 112
Msa1357240 AT3G25640.2 35.260 173 93 6 81 238 124 292 1.68e-29 112
Msa1357240 AT5G23100.1 31.443 194 94 5 79 238 88 276 1.17e-25 101

Find 39 sgRNAs with CRISPR-Local

Find 46 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTGGTACTGGTGTTATAAA+TGG 0.255094 tig0016006:-36906 Msa1357240:CDS
ACAATTACTGGCACGATATT+CGG 0.320130 tig0016006:-37209 Msa1357240:CDS
TCCTTTGCTAAAACACTTGT+TGG 0.329615 tig0016006:+37104 None:intergenic
ATGGATATTTGTTATAGTTT+TGG 0.334698 tig0016006:+37261 None:intergenic
GCAAGTTCTAAAAGAAGAAT+AGG 0.336410 tig0016006:+37131 None:intergenic
GTCTTGTTCTTGTTCTACTA+AGG 0.338250 tig0016006:+37313 None:intergenic
CAAGTTCTAAAAGAAGAATA+GGG 0.363193 tig0016006:+37132 None:intergenic
TTTGGATTATGTATTGTAAT+GGG 0.364697 tig0016006:-37007 Msa1357240:CDS
ATGACATCTACAACAATTAC+TGG 0.370669 tig0016006:-37221 Msa1357240:CDS
CTTTGGATTATGTATTGTAA+TGG 0.380120 tig0016006:-37008 Msa1357240:CDS
TCTTGACCAATGCTTCCTTC+AGG 0.407088 tig0016006:+36777 None:intergenic
GCTTCTAAATCACTTCTTGA+AGG 0.409476 tig0016006:+36954 None:intergenic
CGGTCGGTTTCTGTTGGTAC+TGG 0.430039 tig0016006:-36918 Msa1357240:CDS
TAAAAGAAGAATAGGGTTTG+TGG 0.440606 tig0016006:+37139 None:intergenic
TGGGAGGAAAGTCGGGTATT+CGG 0.443287 tig0016006:-36988 Msa1357240:CDS
CTAAGGTGCAGTTGCAAGTA+GGG 0.450004 tig0016006:+37330 None:intergenic
ATTGTAATGGGAGGAAAGTC+GGG 0.463638 tig0016006:-36995 Msa1357240:CDS
TCTTAGAGCTAACTTTCAAA+GGG 0.464488 tig0016006:-36847 Msa1357240:CDS
ATCTTAGAGCTAACTTTCAA+AGG 0.465935 tig0016006:-36848 Msa1357240:CDS
TATTGTAATGGGAGGAAAGT+CGG 0.482311 tig0016006:-36996 Msa1357240:CDS
ATTGATCCTGAAGGAAGCAT+TGG 0.506241 tig0016006:-36783 Msa1357240:CDS
GCTAACTTTCAAAGGGTTCG+TGG 0.509016 tig0016006:-36840 Msa1357240:CDS
ACGATATTCGGATACCGTAA+AGG 0.517456 tig0016006:-37197 Msa1357240:CDS
ACTACCAACCTTGGTGATGT+AGG 0.531843 tig0016006:+37442 None:intergenic
ATCCACTGTGGTTACACCAT+TGG 0.548210 tig0016006:+37409 None:intergenic
TTGATGCGGTCGGTTTCTGT+TGG 0.551122 tig0016006:-36924 Msa1357240:CDS
ACTAAGGTGCAGTTGCAAGT+AGG 0.551502 tig0016006:+37329 None:intergenic
ATGAGTGAAGAATGTGATGA+TGG 0.567861 tig0016006:+37242 None:intergenic
GGATTATGTATTGTAATGGG+AGG 0.573929 tig0016006:-37004 Msa1357240:CDS
TAGAAGCACTTAACTTGATG+CGG 0.577344 tig0016006:-36938 Msa1357240:CDS
TTTCATTTGATTGATCCTGA+AGG 0.587099 tig0016006:-36792 Msa1357240:CDS
ATGGCCTACATCACCAAGGT+TGG 0.591227 tig0016006:-37446 Msa1357240:CDS
AGCACTTAACTTGATGCGGT+CGG 0.611210 tig0016006:-36934 Msa1357240:CDS
GGTAGTAGCAGCATGACCAA+TGG 0.630024 tig0016006:-37425 Msa1357240:CDS
CATGCTGCTACTACCAACCT+TGG 0.631697 tig0016006:+37433 None:intergenic
TTACAATACATAATCCAAAG+AGG 0.638246 tig0016006:+37011 None:intergenic
TTAGCAAAGGAAATGAGAGG+AGG 0.650662 tig0016006:-37092 Msa1357240:CDS
GACAATGGCCTACATCACCA+AGG 0.711681 tig0016006:-37450 None:intergenic
GACCAATGGTGTAACCACAG+TGG 0.740457 tig0016006:-37411 Msa1357240:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATGGATATTTGTTATAGTTT+TGG + tig0016006:36934-36953 None:intergenic 20.0%
!!! AAAAACTTACTTTTCCTTTA+CGG + tig0016006:37012-37031 None:intergenic 20.0%
!! TTTGGATTATGTATTGTAAT+GGG - tig0016006:37185-37204 Msa1357240:CDS 20.0%
!!! GTTTTGAAATGCTAATATCT+TGG + tig0016006:36904-36923 None:intergenic 25.0%
!!! CAAGTTCTAAAAGAAGAATA+GGG + tig0016006:37063-37082 None:intergenic 25.0%
! TTACAATACATAATCCAAAG+AGG + tig0016006:37184-37203 None:intergenic 25.0%
! CTTTGGATTATGTATTGTAA+TGG - tig0016006:37184-37203 Msa1357240:CDS 25.0%
ATGACATCTACAACAATTAC+TGG - tig0016006:36971-36990 Msa1357240:CDS 30.0%
TAAAAGAAGAATAGGGTTTG+TGG + tig0016006:37056-37075 None:intergenic 30.0%
!! GCAAGTTCTAAAAGAAGAAT+AGG + tig0016006:37064-37083 None:intergenic 30.0%
ATCTTAGAGCTAACTTTCAA+AGG - tig0016006:37344-37363 Msa1357240:CDS 30.0%
TCTTAGAGCTAACTTTCAAA+GGG - tig0016006:37345-37364 Msa1357240:CDS 30.0%
TTTCATTTGATTGATCCTGA+AGG - tig0016006:37400-37419 Msa1357240:CDS 30.0%
!!! TTGTTTTTGACAATCCACTG+TGG + tig0016006:36798-36817 None:intergenic 35.0%
GTCAAAAACAAGTTCGATCA+TGG - tig0016006:36807-36826 Msa1357240:CDS 35.0%
AAAAACAAGTTCGATCATGG+AGG - tig0016006:36810-36829 Msa1357240:CDS 35.0%
! GTCTTGTTCTTGTTCTACTA+AGG + tig0016006:36882-36901 None:intergenic 35.0%
ATGAGTGAAGAATGTGATGA+TGG + tig0016006:36953-36972 None:intergenic 35.0%
ACAATTACTGGCACGATATT+CGG - tig0016006:36983-37002 Msa1357240:CDS 35.0%
!!! TCCAACAAGTGTTTTAGCAA+AGG - tig0016006:37087-37106 Msa1357240:CDS 35.0%
TCCTTTGCTAAAACACTTGT+TGG + tig0016006:37091-37110 None:intergenic 35.0%
!! GTTTTAGCAAAGGAAATGAG+AGG - tig0016006:37097-37116 Msa1357240:CDS 35.0%
!! ACTTGTTTTCAACTCCTCTT+TGG - tig0016006:37167-37186 Msa1357240:CDS 35.0%
! GGATTATGTATTGTAATGGG+AGG - tig0016006:37188-37207 Msa1357240:CDS 35.0%
! TATTGTAATGGGAGGAAAGT+CGG - tig0016006:37196-37215 Msa1357240:CDS 35.0%
GCTTCTAAATCACTTCTTGA+AGG + tig0016006:37241-37260 None:intergenic 35.0%
!! TAGAAGCACTTAACTTGATG+CGG - tig0016006:37254-37273 Msa1357240:CDS 35.0%
!! GTTGGTACTGGTGTTATAAA+TGG - tig0016006:37286-37305 Msa1357240:CDS 35.0%
ACGATATTCGGATACCGTAA+AGG - tig0016006:36995-37014 Msa1357240:CDS 40.0%
TTAGCAAAGGAAATGAGAGG+AGG - tig0016006:37100-37119 Msa1357240:CDS 40.0%
! ATTGTAATGGGAGGAAAGTC+GGG - tig0016006:37197-37216 Msa1357240:CDS 40.0%
! ATTGATCCTGAAGGAAGCAT+TGG - tig0016006:37409-37428 Msa1357240:CDS 40.0%
!! ACTACCAACCTTGGTGATGT+AGG + tig0016006:36753-36772 None:intergenic 45.0%
ATCCACTGTGGTTACACCAT+TGG + tig0016006:36786-36805 None:intergenic 45.0%
CTAAGGTGCAGTTGCAAGTA+GGG + tig0016006:36865-36884 None:intergenic 45.0%
ACTAAGGTGCAGTTGCAAGT+AGG + tig0016006:36866-36885 None:intergenic 45.0%
! AGCACTTAACTTGATGCGGT+CGG - tig0016006:37258-37277 Msa1357240:CDS 45.0%
GCTAACTTTCAAAGGGTTCG+TGG - tig0016006:37352-37371 Msa1357240:CDS 45.0%
TCTTGACCAATGCTTCCTTC+AGG + tig0016006:37418-37437 None:intergenic 45.0%
ATGGCCTACATCACCAAGGT+TGG - tig0016006:36746-36765 Msa1357240:CDS 50.0%
CATGCTGCTACTACCAACCT+TGG + tig0016006:36762-36781 None:intergenic 50.0%
GGTAGTAGCAGCATGACCAA+TGG - tig0016006:36767-36786 Msa1357240:CDS 50.0%
GACCAATGGTGTAACCACAG+TGG - tig0016006:36781-36800 Msa1357240:CDS 50.0%
TGGGAGGAAAGTCGGGTATT+CGG - tig0016006:37204-37223 Msa1357240:CDS 50.0%
! TTGATGCGGTCGGTTTCTGT+TGG - tig0016006:37268-37287 Msa1357240:CDS 50.0%
! CGGTCGGTTTCTGTTGGTAC+TGG - tig0016006:37274-37293 Msa1357240:CDS 55.0%
Chromosome Type Strat End Strand Name
tig0016006 gene 36746 37468 36746 ID=Msa1357240;Name=Msa1357240
tig0016006 mRNA 36746 37468 36746 ID=Msa1357240-mRNA-1;Parent=Msa1357240;Name=Msa1357240-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|-1|0|1|-1|1|1|0|240
tig0016006 exon 36746 37468 36746 ID=Msa1357240-mRNA-1:exon:10316;Parent=Msa1357240-mRNA-1
tig0016006 CDS 36746 37468 36746 ID=Msa1357240-mRNA-1:cds;Parent=Msa1357240-mRNA-1
Gene Sequence

>Msa1357240

ATGGCCTACATCACCAAGGTTGGTAGTAGCAGCATGACCAATGGTGTAACCACAGTGGATTGTCAAAAACAAGTTCGATCATGGAGGCTTCTACGTTCACTCATTGAACTACTAATCCCTACTTGCAACTGCACCTTAGTAGAACAAGAACAAGACCAAGATATTAGCATTTCAAAACACTCTCACCAAAACTATAACAAATATCCATCATCACATTCTTCACTCATGACATCTACAACAATTACTGGCACGATATTCGGATACCGTAAAGGAAAAGTAAGTTTTTGTATTCAATCTAACTCAAATTCCACAAACCCTATTCTTCTTTTAGAACTTGCAATTCCAACAAGTGTTTTAGCAAAGGAAATGAGAGGAGGAACGTTGAGAATTGTGCTAGAAAGTGCTTGTAACAATAACAGTAACTTGTTTTCAACTCCTCTTTGGATTATGTATTGTAATGGGAGGAAAGTCGGGTATTCGGTTAAGCGTAAGCCTTCAAGAAGTGATTTAGAAGCACTTAACTTGATGCGGTCGGTTTCTGTTGGTACTGGTGTTATAAATGGAAAAGACATTTGTCAAGAAGATGATCAACTTATGTATCTTAGAGCTAACTTTCAAAGGGTTCGTGGTTCTTCTAAATCTAACTGTGAATCTTTTCATTTGATTGATCCTGAAGGAAGCATTGGTCAAGAGCTTAGTATTTTCTTTTTTCAATCAAAGTGA

Protein sequence

>Msa1357240

MAYITKVGSSSMTNGVTTVDCQKQVRSWRLLRSLIELLIPTCNCTLVEQEQDQDISISKHSHQNYNKYPSSHSSLMTSTTITGTIFGYRKGKVSFCIQSNSNSTNPILLLELAIPTSVLAKEMRGGTLRIVLESACNNNSNLFSTPLWIMYCNGRKVGYSVKRKPSRSDLEALNLMRSVSVGTGVINGKDICQEDDQLMYLRANFQRVRGSSKSNCESFHLIDPEGSIGQELSIFFFQSK*