AlfalfaGEDB | Msa1433150

M. sativa cultivar zhongmu-4 / Msa1433150

KEGG: K20815
GO: -
PFAM: PF06916.16

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1433150	XP_013449388.1	99.213	127	1	0	1	127	1	127	4.82e-85	254

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1433150	A0A072U1Z4	99.213	127	1	0	1	127	1	127	2.30e-85	254

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0142220	Msa1433150	-0.823973	1.037888e-53	-8.615850e-47
Msa0344690	Msa1433150	0.812325	4.488457e-51	-8.615850e-47
Msa0350870	Msa1433150	-0.824085	9.771485e-54	-8.615850e-47
Msa0381600	Msa1433150	0.812127	4.957304e-51	-8.615850e-47
Msa0436800	Msa1433150	-0.800456	1.428819e-48	-8.615850e-47
Msa0437830	Msa1433150	0.814439	1.539701e-51	-8.615850e-47
Msa0443290	Msa1433150	-0.819374	1.201250e-52	-8.615850e-47
Msa0469940	Msa1433150	0.819001	1.460507e-52	-8.615850e-47
Msa0490870	Msa1433150	-0.805124	1.552978e-49	-8.615850e-47
Msa0492880	Msa1433150	-0.810208	1.292214e-50	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1433150	MtrunA17_Chr7g0250101	99.213	127	1	0	1	127	1	127	4.43e-89	254

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1433150	AT2G20940.1	60.800	125	46	1	5	126	4	128	8.95e-52	160
Msa1433150	AT2G20940.2	60.800	125	46	1	5	126	4	128	8.95e-52	160

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Find 36 sgRNAs with CRISPR-Local

Find 46 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACGTCGACGTTGTTTCTAA+TGG	0.376811	tig0027235:-28371	None:intergenic
AAGAATCAGTATCAGAATTA+AGG	0.347381	tig0027235:-28450	None:intergenic
AAGTACGGAAAAGTAGCAAT+CGG	0.518083	tig0027235:+28301	Msa1433150:CDS
AATCCTCGAGAAATTTCACT+TGG	0.549671	tig0027235:+28399	Msa1433150:CDS
ACAAGGCTCTGATTCCTGTT+CGG	0.366143	tig0027235:+28551	Msa1433150:CDS
AGGAGGAAGATTGTGAAGAC+TGG	0.542945	tig0027235:+28622	Msa1433150:CDS
ATAGTGATGGGAACCCGAAC+AGG	0.445276	tig0027235:-28565	None:intergenic
ATCCTCGAGAAATTTCACTT+GGG	0.664906	tig0027235:+28400	Msa1433150:CDS
CAAGGCTCTGATTCCTGTTC+GGG	0.317971	tig0027235:+28552	Msa1433150:CDS
CAATTGCGAGGTTCTTGGCG+AGG	0.600349	tig0027235:+28602	Msa1433150:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CAGTATCAGAATTAAGGTTA+GGG	-	tig0027235:28447-28466	None:intergenic	30.0%
TCAGTATCAGAATTAAGGTT+AGG	-	tig0027235:28448-28467	None:intergenic	30.0%
AAGTACGGAAAAGTAGCAAT+CGG	+	tig0027235:28301-28320	Msa1433150:CDS	35.0%
AATCCTCGAGAAATTTCACT+TGG	+	tig0027235:28399-28418	Msa1433150:CDS	35.0%
ATCCTCGAGAAATTTCACTT+GGG	+	tig0027235:28400-28419	Msa1433150:CDS	35.0%
CACCCAAGTGAAATTTCTCG+AGG	-	tig0027235:28405-28424	None:intergenic	45.0%
CTTGTTGCAAAGCACAGCTA+GGG	-	tig0027235:28536-28555	None:intergenic	45.0%
AGGAGGAAGATTGTGAAGAC+TGG	+	tig0027235:28622-28641	Msa1433150:CDS	45.0%
CGCGAGCTCCTGAAAAAGTA+CGG	+	tig0027235:28286-28305	Msa1433150:CDS	50.0%
TCTAATGGCGACGTAGATGC+CGG	-	tig0027235:28359-28378	None:intergenic	50.0%

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Chromosome	Type	Strat	End	Strand	Name
tig0027235	gene	28268	28651	28268	ID=Msa1433150;Name=Msa1433150
tig0027235	mRNA	28268	28651	28268	ID=Msa1433150-mRNA-1;Parent=Msa1433150;Name=Msa1433150-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|127
tig0027235	exon	28268	28651	28268	ID=Msa1433150-mRNA-1:exon:8476;Parent=Msa1433150-mRNA-1
tig0027235	CDS	28268	28651	28268	ID=Msa1433150-mRNA-1:cds;Parent=Msa1433150-mRNA-1

Gene Sequence

>Msa1433150

ATGTCCGGTGGTCGATTCCGCGAGCTCCTGAAAAAGTACGGAAAAGTAGCAATCGGTGTTCACTGCTCCGTTTCCGCCGCTTCCGTCGCCGGCATCTACGTCGCCATTAGAAACAACGTCGACGTTGAAGCAATCCTCGAGAAATTTCACTTGGGTGCCGCTTCTCCCGAAGAAAACCCTAACCTTAATTCTGATACTGATTCTTCCACCGTGCCAGTGAAGAATCGCACGGCGCAGATGGCGGCTTCTGCTGGTGGTGCTTTTACCCTAGCTGTGCTTTGCAACAAGGCTCTGATTCCTGTTCGGGTTCCCATCACTATAGCACTCACGCCCCCAATTGCGAGGTTCTTGGCGAGGAGGAAGATTGTGAAGACTGGTGTATGA

Protein sequence

>Msa1433150

MSGGRFRELLKKYGKVAIGVHCSVSAASVAGIYVAIRNNVDVEAILEKFHLGAASPEENPNLNSDTDSSTVPVKNRTAQMAASAGGAFTLAVLCNKALIPVRVPITIALTPPIARFLARRKIVKTGV*