Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1433150 | XP_013449388.1 | 99.213 | 127 | 1 | 0 | 1 | 127 | 1 | 127 | 4.82e-85 | 254 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1433150 | A0A072U1Z4 | 99.213 | 127 | 1 | 0 | 1 | 127 | 1 | 127 | 2.30e-85 | 254 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa1433150 | Msa1461810 | 0.838062 | 3.595240e-57 | -8.615850e-47 |
| Msa0766630 | Msa1433150 | -0.805025 | 1.629085e-49 | -8.615850e-47 |
| Msa0142220 | Msa1433150 | -0.823973 | 1.037888e-53 | -8.615850e-47 |
| Msa0381600 | Msa1433150 | 0.812127 | 4.957304e-51 | -8.615850e-47 |
| Msa0436800 | Msa1433150 | -0.800456 | 1.428819e-48 | -8.615850e-47 |
| Msa0437830 | Msa1433150 | 0.814439 | 1.539701e-51 | -8.615850e-47 |
| Msa0443290 | Msa1433150 | -0.819374 | 1.201250e-52 | -8.615850e-47 |
| Msa0469940 | Msa1433150 | 0.819001 | 1.460507e-52 | -8.615850e-47 |
| Msa1037620 | Msa1433150 | -0.803437 | 3.488386e-49 | -8.615850e-47 |
| Msa1041600 | Msa1433150 | 0.838808 | 2.307141e-57 | -8.615850e-47 |
| Msa1068200 | Msa1433150 | 0.821345 | 4.242618e-53 | -8.615850e-47 |
| Msa1075220 | Msa1433150 | 0.832070 | 1.165624e-55 | -8.615850e-47 |
| Msa0344690 | Msa1433150 | 0.812325 | 4.488457e-51 | -8.615850e-47 |
| Msa0350870 | Msa1433150 | -0.824085 | 9.771485e-54 | -8.615850e-47 |
| Msa1139000 | Msa1433150 | 0.838429 | 2.890584e-57 | -8.615850e-47 |
| Msa1144720 | Msa1433150 | 0.810707 | 1.008065e-50 | -8.615850e-47 |
| Msa1156340 | Msa1433150 | 0.828106 | 1.081247e-54 | -8.615850e-47 |
| Msa1162550 | Msa1433150 | 0.816103 | 6.571345e-52 | -8.615850e-47 |
| Msa1166500 | Msa1433150 | 0.837623 | 4.659406e-57 | -8.615850e-47 |
| Msa1184530 | Msa1433150 | -0.801985 | 6.952670e-49 | -8.615850e-47 |
| Msa1184550 | Msa1433150 | -0.819424 | 1.169744e-52 | -8.615850e-47 |
| Msa0490870 | Msa1433150 | -0.805124 | 1.552978e-49 | -8.615850e-47 |
| Msa0492880 | Msa1433150 | -0.810208 | 1.292214e-50 | -8.615850e-47 |
| Msa0498220 | Msa1433150 | -0.808937 | 2.423327e-50 | -8.615850e-47 |
| Msa0526100 | Msa1433150 | 0.816948 | 4.249958e-52 | -8.615850e-47 |
| Msa0532810 | Msa1433150 | 0.830077 | 3.598625e-55 | -8.615850e-47 |
| Msa0879550 | Msa1433150 | 0.805685 | 1.184915e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1433150 | MtrunA17_Chr7g0250101 | 99.213 | 127 | 1 | 0 | 1 | 127 | 1 | 127 | 4.43e-89 | 254 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1433150 | AT2G20940.1 | 60.800 | 125 | 46 | 1 | 5 | 126 | 4 | 128 | 8.95e-52 | 160 |
| Msa1433150 | AT2G20940.2 | 60.800 | 125 | 46 | 1 | 5 | 126 | 4 | 128 | 8.95e-52 | 160 |
Find 36 sgRNAs with CRISPR-Local
Find 46 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAAGGCTCTGATTCCTGTTC+GGG | 0.317971 | tig0027235:+28552 | Msa1433150:CDS |
| GCGCAGATGGCGGCTTCTGC+TGG | 0.326079 | tig0027235:+28499 | Msa1433150:CDS |
| CTCGCCAAGAACCTCGCAAT+TGG | 0.328407 | tig0027235:-28601 | None:intergenic |
| TCGCCAAGAACCTCGCAATT+GGG | 0.333134 | tig0027235:-28600 | None:intergenic |
| AAGAATCAGTATCAGAATTA+AGG | 0.347381 | tig0027235:-28450 | None:intergenic |
| ACAAGGCTCTGATTCCTGTT+CGG | 0.366143 | tig0027235:+28551 | Msa1433150:CDS |
| TCAGTATCAGAATTAAGGTT+AGG | 0.368311 | tig0027235:-28445 | None:intergenic |
| AACGTCGACGTTGTTTCTAA+TGG | 0.376811 | tig0027235:-28371 | None:intergenic |
| GGCGACGGAAGCGGCGGAAA+CGG | 0.412713 | tig0027235:-28335 | None:intergenic |
| ATAGTGATGGGAACCCGAAC+AGG | 0.445276 | tig0027235:-28565 | None:intergenic |
| GTTTCCGCCGCTTCCGTCGC+CGG | 0.450661 | tig0027235:+28337 | Msa1433150:CDS |
| CAGTATCAGAATTAAGGTTA+GGG | 0.462709 | tig0027235:-28444 | None:intergenic |
| CGCCAAGAACCTCGCAATTG+GGG | 0.473782 | tig0027235:-28599 | None:intergenic |
| CAGATGGCGGCTTCTGCTGG+TGG | 0.491218 | tig0027235:+28502 | Msa1433150:CDS |
| CCTAGCTGTGCTTTGCAACA+AGG | 0.506591 | tig0027235:+28534 | Msa1433150:CDS |
| CCTTGTTGCAAAGCACAGCT+AGG | 0.511786 | tig0027235:-28534 | None:intergenic |
| AAGTACGGAAAAGTAGCAAT+CGG | 0.518083 | tig0027235:+28301 | Msa1433150:CDS |
| GTAGATGCCGGCGACGGAAG+CGG | 0.518756 | tig0027235:-28344 | None:intergenic |
| GAAGAATCGCACGGCGCAGA+TGG | 0.519084 | tig0027235:+28486 | Msa1433150:CDS |
| AGGAGGAAGATTGTGAAGAC+TGG | 0.542945 | tig0027235:+28622 | Msa1433150:CDS |
| AATCCTCGAGAAATTTCACT+TGG | 0.549671 | tig0027235:+28399 | Msa1433150:CDS |
| TCTAATGGCGACGTAGATGC+CGG | 0.552367 | tig0027235:-28356 | None:intergenic |
| GGGCGTGAGTGCTATAGTGA+TGG | 0.566302 | tig0027235:-28578 | None:intergenic |
| GAATCGCACGGCGCAGATGG+CGG | 0.577526 | tig0027235:+28489 | Msa1433150:CDS |
| CTTGTTGCAAAGCACAGCTA+GGG | 0.588257 | tig0027235:-28533 | None:intergenic |
| CGTGCGATTCTTCACTGGCA+CGG | 0.590678 | tig0027235:-28476 | None:intergenic |
| GGCGACGTAGATGCCGGCGA+CGG | 0.595129 | tig0027235:-28350 | None:intergenic |
| CAATTGCGAGGTTCTTGGCG+AGG | 0.600349 | tig0027235:+28602 | Msa1433150:CDS |
| GATGCCGGCGACGGAAGCGG+CGG | 0.604989 | tig0027235:-28341 | None:intergenic |
| TTGCGAGGTTCTTGGCGAGG+AGG | 0.610969 | tig0027235:+28605 | Msa1433150:CDS |
| CACCCAAGTGAAATTTCTCG+AGG | 0.622021 | tig0027235:-28402 | None:intergenic |
| GCCAAGAACCTCGCAATTGG+GGG | 0.628146 | tig0027235:-28598 | None:intergenic |
| GGCGTGAGTGCTATAGTGAT+GGG | 0.635390 | tig0027235:-28577 | None:intergenic |
| ATCCTCGAGAAATTTCACTT+GGG | 0.664906 | tig0027235:+28400 | Msa1433150:CDS |
| CGTGCCAGTGAAGAATCGCA+CGG | 0.698432 | tig0027235:+28477 | Msa1433150:CDS |
| GCGATTCTTCACTGGCACGG+TGG | 0.698621 | tig0027235:-28473 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAGAATCAGTATCAGAATTA+AGG | - | tig0027235:28453-28472 | None:intergenic | 25.0% |
| ! | ATTAAGGTTAGGGTTTTCTT+CGG | - | tig0027235:28437-28456 | None:intergenic | 30.0% |
| CAGTATCAGAATTAAGGTTA+GGG | - | tig0027235:28447-28466 | None:intergenic | 30.0% | |
| TCAGTATCAGAATTAAGGTT+AGG | - | tig0027235:28448-28467 | None:intergenic | 30.0% | |
| !!! | CTACTTTTCCGTACTTTTTC+AGG | - | tig0027235:28297-28316 | None:intergenic | 35.0% |
| AAGTACGGAAAAGTAGCAAT+CGG | + | tig0027235:28301-28320 | Msa1433150:CDS | 35.0% | |
| AATCCTCGAGAAATTTCACT+TGG | + | tig0027235:28399-28418 | Msa1433150:CDS | 35.0% | |
| ATCCTCGAGAAATTTCACTT+GGG | + | tig0027235:28400-28419 | Msa1433150:CDS | 35.0% | |
| ! | TTAAGGTTAGGGTTTTCTTC+GGG | - | tig0027235:28436-28455 | None:intergenic | 35.0% |
| !! | AACGTCGACGTTGTTTCTAA+TGG | - | tig0027235:28374-28393 | None:intergenic | 40.0% |
| CACCCAAGTGAAATTTCTCG+AGG | - | tig0027235:28405-28424 | None:intergenic | 45.0% | |
| CTTGTTGCAAAGCACAGCTA+GGG | - | tig0027235:28536-28555 | None:intergenic | 45.0% | |
| ! | ACAAGGCTCTGATTCCTGTT+CGG | + | tig0027235:28551-28570 | Msa1433150:CDS | 45.0% |
| AGGAGGAAGATTGTGAAGAC+TGG | + | tig0027235:28622-28641 | Msa1433150:CDS | 45.0% | |
| !!! | CGTACTTTTTCAGGAGCTCG+CGG | - | tig0027235:28288-28307 | None:intergenic | 50.0% |
| CGCGAGCTCCTGAAAAAGTA+CGG | + | tig0027235:28286-28305 | Msa1433150:CDS | 50.0% | |
| TCTAATGGCGACGTAGATGC+CGG | - | tig0027235:28359-28378 | None:intergenic | 50.0% | |
| ! | AGGGTTTTCTTCGGGAGAAG+CGG | - | tig0027235:28428-28447 | None:intergenic | 50.0% |
| CCTTGTTGCAAAGCACAGCT+AGG | - | tig0027235:28537-28556 | None:intergenic | 50.0% | |
| CCTAGCTGTGCTTTGCAACA+AGG | + | tig0027235:28534-28553 | Msa1433150:CDS | 50.0% | |
| ! | CAAGGCTCTGATTCCTGTTC+GGG | + | tig0027235:28552-28571 | Msa1433150:CDS | 50.0% |
| ! | ATAGTGATGGGAACCCGAAC+AGG | - | tig0027235:28568-28587 | None:intergenic | 50.0% |
| GGCGTGAGTGCTATAGTGAT+GGG | - | tig0027235:28580-28599 | None:intergenic | 50.0% | |
| TCGCCAAGAACCTCGCAATT+GGG | - | tig0027235:28603-28622 | None:intergenic | 50.0% | |
| CGTGCGATTCTTCACTGGCA+CGG | - | tig0027235:28479-28498 | None:intergenic | 55.0% | |
| CGTGCCAGTGAAGAATCGCA+CGG | + | tig0027235:28477-28496 | Msa1433150:CDS | 55.0% | |
| GGGCGTGAGTGCTATAGTGA+TGG | - | tig0027235:28581-28600 | None:intergenic | 55.0% | |
| GCCAAGAACCTCGCAATTGG+GGG | - | tig0027235:28601-28620 | None:intergenic | 55.0% | |
| CGCCAAGAACCTCGCAATTG+GGG | - | tig0027235:28602-28621 | None:intergenic | 55.0% | |
| CTCGCCAAGAACCTCGCAAT+TGG | - | tig0027235:28604-28623 | None:intergenic | 55.0% | |
| CAATTGCGAGGTTCTTGGCG+AGG | + | tig0027235:28602-28621 | Msa1433150:CDS | 55.0% | |
| GCGATTCTTCACTGGCACGG+TGG | - | tig0027235:28476-28495 | None:intergenic | 60.0% | |
| TGCGCCGTGCGATTCTTCAC+TGG | - | tig0027235:28484-28503 | None:intergenic | 60.0% | |
| GAAGAATCGCACGGCGCAGA+TGG | + | tig0027235:28486-28505 | Msa1433150:CDS | 60.0% | |
| GCCCCCAATTGCGAGGTTCT+TGG | + | tig0027235:28597-28616 | Msa1433150:CDS | 60.0% | |
| TTGCGAGGTTCTTGGCGAGG+AGG | + | tig0027235:28605-28624 | Msa1433150:CDS | 60.0% | |
| GAGCTCGCGGAATCGACCAC+CGG | - | tig0027235:28275-28294 | None:intergenic | 65.0% | |
| GTAGATGCCGGCGACGGAAG+CGG | - | tig0027235:28347-28366 | None:intergenic | 65.0% | |
| GAATCGCACGGCGCAGATGG+CGG | + | tig0027235:28489-28508 | Msa1433150:CDS | 65.0% | |
| ! | CAGATGGCGGCTTCTGCTGG+TGG | + | tig0027235:28502-28521 | Msa1433150:CDS | 65.0% |
| CACTCACGCCCCCAATTGCG+AGG | + | tig0027235:28590-28609 | Msa1433150:CDS | 65.0% | |
| GGCGACGGAAGCGGCGGAAA+CGG | - | tig0027235:28338-28357 | None:intergenic | 70.0% | |
| GTTTCCGCCGCTTCCGTCGC+CGG | + | tig0027235:28337-28356 | Msa1433150:CDS | 70.0% | |
| GGCGACGTAGATGCCGGCGA+CGG | - | tig0027235:28353-28372 | None:intergenic | 70.0% | |
| ! | GCGCAGATGGCGGCTTCTGC+TGG | + | tig0027235:28499-28518 | Msa1433150:CDS | 70.0% |
| GATGCCGGCGACGGAAGCGG+CGG | - | tig0027235:28344-28363 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0027235 | gene | 28268 | 28651 | 28268 | ID=Msa1433150;Name=Msa1433150 |
| tig0027235 | mRNA | 28268 | 28651 | 28268 | ID=Msa1433150-mRNA-1;Parent=Msa1433150;Name=Msa1433150-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|127 |
| tig0027235 | exon | 28268 | 28651 | 28268 | ID=Msa1433150-mRNA-1:exon:8476;Parent=Msa1433150-mRNA-1 |
| tig0027235 | CDS | 28268 | 28651 | 28268 | ID=Msa1433150-mRNA-1:cds;Parent=Msa1433150-mRNA-1 |
| Gene Sequence |
| Protein sequence |
