Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene006754.t1 | XP_004507193.1 | 93.8 | 65 | 4 | 0 | 1 | 65 | 1 | 65 | 2.80E-24 | 120.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene006754.t1 | P46302 | 89.2 | 65 | 7 | 0 | 1 | 65 | 1 | 65 | 3.4e-23 | 108.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene006754.t1 | A0A1S2YMY4 | 93.8 | 65 | 4 | 0 | 1 | 65 | 1 | 65 | 2.0e-24 | 120.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene028153 | MS.gene006754 | PPI |
| MS.gene87287 | MS.gene006754 | PPI |
| MS.gene006754 | MS.gene50744 | PPI |
| MS.gene006754 | MS.gene031256 | PPI |
| MS.gene006754 | MS.gene072782 | PPI |
| MS.gene006754 | MS.gene72768 | PPI |
| MS.gene006754 | MS.gene96825 | PPI |
| MS.gene69814 | MS.gene006754 | PPI |
| MS.gene006754 | MS.gene23314 | PPI |
| MS.gene006754 | MS.gene82426 | PPI |
| MS.gene87285 | MS.gene006754 | PPI |
| MS.gene031254 | MS.gene006754 | PPI |
| MS.gene006754 | MS.gene015586 | PPI |
| MS.gene031549 | MS.gene006754 | PPI |
| MS.gene006754 | MS.gene019309 | PPI |
| MS.gene006754 | MS.gene99823 | PPI |
| MS.gene003693 | MS.gene006754 | PPI |
| MS.gene006754 | MS.gene86059 | PPI |
| MS.gene006754 | MS.gene83444 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene006754.t1 | MTR_4g081180 | 93.846 | 65 | 4 | 0 | 1 | 65 | 150 | 214 | 7.98e-38 | 124 |
| MS.gene006754.t1 | MTR_6g013210 | 90.769 | 65 | 6 | 0 | 1 | 65 | 1 | 65 | 1.60e-37 | 119 |
| MS.gene006754.t1 | MTR_6g013410 | 75.385 | 65 | 15 | 1 | 1 | 65 | 38 | 101 | 7.47e-28 | 96.3 |
| MS.gene006754.t1 | MTR_8g089765 | 70.000 | 60 | 18 | 0 | 1 | 60 | 1 | 60 | 2.25e-25 | 90.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene006754.t1 | AT5G03850 | 87.692 | 65 | 7 | 1 | 1 | 65 | 1 | 64 | 2.56e-34 | 111 |
| MS.gene006754.t1 | AT3G10090 | 87.692 | 65 | 7 | 1 | 1 | 65 | 1 | 64 | 2.56e-34 | 111 |
| MS.gene006754.t1 | AT5G64140 | 87.692 | 65 | 7 | 1 | 1 | 65 | 1 | 64 | 5.01e-34 | 110 |
Find 22 sgRNAs with CRISPR-Local
Find 22 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGGGTGTCTCCTTCTCTAAC+TGG | 0.224700 | 4.1:-25674236 | None:intergenic |
| TGCTGTTGTTGTCAAGGTTA+TGG | 0.305748 | 4.1:+25674124 | MS.gene006754:CDS |
| GCTGTTGTTGTCAAGGTTAT+GGG | 0.344081 | 4.1:+25674125 | MS.gene006754:CDS |
| GAGGACAGGTGACTCAAGTT+AGG | 0.354183 | 4.1:+25674162 | MS.gene006754:CDS |
| TTCCTCATGATGTGACGGTT+CGG | 0.361521 | 4.1:-25674203 | None:intergenic |
| TCCTCATGATGTGACGGTTC+GGG | 0.372907 | 4.1:-25674202 | None:intergenic |
| CTTCTCTTTCAGACTCAAGC+AGG | 0.393026 | 4.1:-25674262 | None:intergenic |
| GTCAAGGTTATGGGTCGTAC+TGG | 0.486004 | 4.1:+25674134 | MS.gene006754:CDS |
| TTTCAGACTCAAGCAGGGTG+AGG | 0.496062 | 4.1:-25674256 | None:intergenic |
| GGGTGTCTCCTTCTCTAACT+GGG | 0.522501 | 4.1:-25674235 | None:intergenic |
| AGGACAGGTGACTCAAGTTA+GGG | 0.535175 | 4.1:+25674163 | MS.gene006754:CDS |
| CAAGTATGCTGTTGTTGTCA+AGG | 0.536125 | 4.1:+25674118 | MS.gene006754:CDS |
| TCGTACTGGTTCTAGAGGAC+AGG | 0.538891 | 4.1:+25674148 | MS.gene006754:CDS |
| TTCAGACTCAAGCAGGGTGA+GGG | 0.547860 | 4.1:-25674255 | None:intergenic |
| ACCCGAACCGTCACATCATG+AGG | 0.555528 | 4.1:+25674201 | MS.gene006754:CDS |
| TTCTCTTTCAGACTCAAGCA+GGG | 0.585301 | 4.1:-25674261 | None:intergenic |
| TCACATCATGAGGAATGTGA+AGG | 0.587194 | 4.1:+25674211 | MS.gene006754:CDS |
| TCACATTCCTCATGATGTGA+CGG | 0.589714 | 4.1:-25674208 | None:intergenic |
| CACATCATGAGGAATGTGAA+GGG | 0.593653 | 4.1:+25674212 | MS.gene006754:CDS |
| GTGAAGGGCCCAGTTAGAGA+AGG | 0.598823 | 4.1:+25674227 | MS.gene006754:CDS |
| AGTCTGAAAGAGAAGCAAGA+AGG | 0.599710 | 4.1:+25674270 | MS.gene006754:CDS |
| ATGGGTCGTACTGGTTCTAG+AGG | 0.630812 | 4.1:+25674143 | MS.gene006754:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AGTCTGAAAGAGAAGCAAGA+AGG | + | chr4.1:25674270-25674289 | MS.gene006754:CDS | 40.0% | |
| CAAGTATGCTGTTGTTGTCA+AGG | + | chr4.1:25674118-25674137 | MS.gene006754:CDS | 40.0% | |
| CACATCATGAGGAATGTGAA+GGG | + | chr4.1:25674212-25674231 | MS.gene006754:CDS | 40.0% | |
| GCTGTTGTTGTCAAGGTTAT+GGG | + | chr4.1:25674125-25674144 | MS.gene006754:CDS | 40.0% | |
| TCACATCATGAGGAATGTGA+AGG | + | chr4.1:25674211-25674230 | MS.gene006754:CDS | 40.0% | |
| TCACATTCCTCATGATGTGA+CGG | - | chr4.1:25674211-25674230 | None:intergenic | 40.0% | |
| TGCTGTTGTTGTCAAGGTTA+TGG | + | chr4.1:25674124-25674143 | MS.gene006754:CDS | 40.0% | |
| TTCTCTTTCAGACTCAAGCA+GGG | - | chr4.1:25674264-25674283 | None:intergenic | 40.0% | |
| AGGACAGGTGACTCAAGTTA+GGG | + | chr4.1:25674163-25674182 | MS.gene006754:CDS | 45.0% | |
| CTTCTCTTTCAGACTCAAGC+AGG | - | chr4.1:25674265-25674284 | None:intergenic | 45.0% | |
| TTCCTCATGATGTGACGGTT+CGG | - | chr4.1:25674206-25674225 | None:intergenic | 45.0% | |
| GAGGACAGGTGACTCAAGTT+AGG | + | chr4.1:25674162-25674181 | MS.gene006754:CDS | 50.0% | |
| GGGTGTCTCCTTCTCTAACT+GGG | - | chr4.1:25674238-25674257 | None:intergenic | 50.0% | |
| GTCAAGGTTATGGGTCGTAC+TGG | + | chr4.1:25674134-25674153 | MS.gene006754:CDS | 50.0% | |
| TCCTCATGATGTGACGGTTC+GGG | - | chr4.1:25674205-25674224 | None:intergenic | 50.0% | |
| TTCAGACTCAAGCAGGGTGA+GGG | - | chr4.1:25674258-25674277 | None:intergenic | 50.0% | |
| TTTCAGACTCAAGCAGGGTG+AGG | - | chr4.1:25674259-25674278 | None:intergenic | 50.0% | |
| ! | AGGGTGTCTCCTTCTCTAAC+TGG | - | chr4.1:25674239-25674258 | None:intergenic | 50.0% |
| !! | ATGGGTCGTACTGGTTCTAG+AGG | + | chr4.1:25674143-25674162 | MS.gene006754:CDS | 50.0% |
| !! | TCGTACTGGTTCTAGAGGAC+AGG | + | chr4.1:25674148-25674167 | MS.gene006754:CDS | 50.0% |
| ACCCGAACCGTCACATCATG+AGG | + | chr4.1:25674201-25674220 | MS.gene006754:CDS | 55.0% | |
| GTGAAGGGCCCAGTTAGAGA+AGG | + | chr4.1:25674227-25674246 | MS.gene006754:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 25674104 | 25674301 | 25674104 | ID=MS.gene006754 |
| chr4.1 | mRNA | 25674104 | 25674301 | 25674104 | ID=MS.gene006754.t1;Parent=MS.gene006754 |
| chr4.1 | exon | 25674104 | 25674301 | 25674104 | ID=MS.gene006754.t1.exon1;Parent=MS.gene006754.t1 |
| chr4.1 | CDS | 25674104 | 25674301 | 25674104 | ID=cds.MS.gene006754.t1;Parent=MS.gene006754.t1 |
| Gene Sequence |
| Protein sequence |
