AlfalfaGEDB | MS.gene031254

M. sativa cultivar XinJiangDaYe / MS.gene031254

KEGG: K02979
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0019538 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576
PFAM: PF01200.21

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene031254.t1	XP_004489433.1	96.9	65	2	0	1	65	1	65	2.50E-25	124

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene031254.t1	P46302	87.7	65	8	0	1	65	1	65	1.2e-23	109.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene031254.t1	Q2HWC2	96.9	65	2	0	1	65	1	65	1.8e-25	124.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene031254	MS.gene87287	PPI
MS.gene031254	MS.gene83444	PPI
MS.gene031254	MS.gene50744	PPI
MS.gene031254	MS.gene031256	PPI
MS.gene031254	MS.gene072782	PPI
MS.gene031254	MS.gene87285	PPI
MS.gene031254	MS.gene003693	PPI
MS.gene031254	MS.gene72768	PPI
MS.gene031254	MS.gene99823	PPI
MS.gene031254	MS.gene96825	PPI
MS.gene031254	MS.gene23314	PPI
MS.gene031254	MS.gene006754	PPI
MS.gene031254	MS.gene82426	PPI
MS.gene87285	MS.gene031254	PPI
MS.gene031254	MS.gene69814	PPI
MS.gene031254	MS.gene028153	PPI
MS.gene031549	MS.gene031254	PPI
MS.gene031254	MS.gene015586	PPI
MS.gene031254	MS.gene019309	PPI
MS.gene031254	MS.gene86059	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene031254.t1	MTR_6g013210	96.923	65	2	0	1	65	1	65	1.82e-40	126
MS.gene031254.t1	MTR_4g081180	87.692	65	8	0	1	65	150	214	1.79e-34	116
MS.gene031254.t1	MTR_6g013410	80.000	65	12	1	1	65	38	101	1.90e-29	100
MS.gene031254.t1	MTR_8g089765	73.333	60	16	0	1	60	1	60	1.81e-25	90.1

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene031254.t1	AT5G03850	87.692	65	7	1	1	65	1	64	3.68e-34	110
MS.gene031254.t1	AT3G10090	87.692	65	7	1	1	65	1	64	3.68e-34	110
MS.gene031254.t1	AT5G64140	86.154	65	8	1	1	65	1	64	1.81e-33	109

Find 17 sgRNAs with CRISPR-Local

Find 18 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GCTATTGTTGTGAAAGTTAT+GGG	0.389099	2.1:+18065687	MS.gene031254:CDS
AGAATGTATCCCTCCCTAAC+TGG	0.428755	2.1:-18065798	None:intergenic
TGCTATTGTTGTGAAAGTTA+TGG	0.460517	2.1:+18065686	MS.gene031254:CDS
GAATGTGAAGGGACCAGTTA+GGG	0.464736	2.1:+18065785	MS.gene031254:CDS
AACCTGAGTGACTTGTCCTC+TGG	0.467654	2.1:-18065721	None:intergenic
GGAATGTGAAGGGACCAGTT+AGG	0.473428	2.1:+18065784	MS.gene031254:CDS
TTGCTTCTCTCTCAGACTCA+AGG	0.498678	2.1:-18065827	None:intergenic
GTGAAAGTTATGGGTCGTAC+TGG	0.530087	2.1:+18065696	MS.gene031254:CDS
TGTGAAGGGACCAGTTAGGG+AGG	0.582336	2.1:+18065788	MS.gene031254:CDS
ATCAGAATCGTCACATCATG+AGG	0.585221	2.1:+18065763	MS.gene031254:CDS
CTTCTCTCTCAGACTCAAGG+AGG	0.589933	2.1:-18065824	None:intergenic
TCACATCATGAGGAATGTGA+AGG	0.626309	2.1:+18065773	MS.gene031254:CDS
CACATCATGAGGAATGTGAA+GGG	0.632733	2.1:+18065774	MS.gene031254:CDS
ATCCAGAGGACAAGTCACTC+AGG	0.638739	2.1:+18065719	MS.gene031254:CDS
TTCTCTCTCAGACTCAAGGA+GGG	0.640280	2.1:-18065823	None:intergenic
GTGAAGGGACCAGTTAGGGA+GGG	0.657315	2.1:+18065789	MS.gene031254:CDS
ATGGGTCGTACTGGATCCAG+AGG	0.663896	2.1:+18065705	MS.gene031254:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	GCTATTGTTGTGAAAGTTAT+GGG	+	chr2.1:18065687-18065706	MS.gene031254:CDS	30.0%
	TGCTATTGTTGTGAAAGTTA+TGG	+	chr2.1:18065686-18065705	MS.gene031254:CDS	30.0%
!	TCAGGTTAGAGTGAAGTTTT+TGG	+	chr2.1:18065737-18065756	MS.gene031254:CDS	35.0%
	ATCAGAATCGTCACATCATG+AGG	+	chr2.1:18065763-18065782	MS.gene031254:CDS	40.0%
	CACATCATGAGGAATGTGAA+GGG	+	chr2.1:18065774-18065793	MS.gene031254:CDS	40.0%
	TCACATCATGAGGAATGTGA+AGG	+	chr2.1:18065773-18065792	MS.gene031254:CDS	40.0%
	AGAATGTATCCCTCCCTAAC+TGG	-	chr2.1:18065801-18065820	None:intergenic	45.0%
	GAATGTGAAGGGACCAGTTA+GGG	+	chr2.1:18065785-18065804	MS.gene031254:CDS	45.0%
	GTGAAAGTTATGGGTCGTAC+TGG	+	chr2.1:18065696-18065715	MS.gene031254:CDS	45.0%
	TTCTCTCTCAGACTCAAGGA+GGG	-	chr2.1:18065826-18065845	None:intergenic	45.0%
	TTGCTTCTCTCTCAGACTCA+AGG	-	chr2.1:18065830-18065849	None:intergenic	45.0%
	ATCCAGAGGACAAGTCACTC+AGG	+	chr2.1:18065719-18065738	MS.gene031254:CDS	50.0%
	CTTCTCTCTCAGACTCAAGG+AGG	-	chr2.1:18065827-18065846	None:intergenic	50.0%
	GGAATGTGAAGGGACCAGTT+AGG	+	chr2.1:18065784-18065803	MS.gene031254:CDS	50.0%
!	AACCTGAGTGACTTGTCCTC+TGG	-	chr2.1:18065724-18065743	None:intergenic	50.0%
	ATGGGTCGTACTGGATCCAG+AGG	+	chr2.1:18065705-18065724	MS.gene031254:CDS	55.0%
	GTGAAGGGACCAGTTAGGGA+GGG	+	chr2.1:18065789-18065808	MS.gene031254:CDS	55.0%
	TGTGAAGGGACCAGTTAGGG+AGG	+	chr2.1:18065788-18065807	MS.gene031254:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.1	gene	18065666	18065863	18065666	ID=MS.gene031254
chr2.1	mRNA	18065666	18065863	18065666	ID=MS.gene031254.t1;Parent=MS.gene031254
chr2.1	exon	18065666	18065863	18065666	ID=MS.gene031254.t1.exon1;Parent=MS.gene031254.t1
chr2.1	CDS	18065666	18065863	18065666	ID=cds.MS.gene031254.t1;Parent=MS.gene031254.t1

Gene Sequence	>MS.gene031254 ATGGAATCACAGATTAAGCATGCTATTGTTGTGAAAGTTATGGGTCGTACTGGATCCAGAGGACAAGTCACTCAGGTTAGAGTGAAGTTTTTGGACGATCAGAATCGTCACATCATGAGGAATGTGAAGGGACCAGTTAGGGAGGGATACATTCTCACCCTCCTTGAGTCTGAGAGAGAAGCAAGAAGACTCCGATAG
Protein sequence	>MS.gene031254.t1 MESQIKHAIVVKVMGRTGSRGQVTQVRVKFLDDQNRHIMRNVKGPVREGYILTLLESEREARRLR

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