Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene031549.t1 | XP_004507193.1 | 93.8 | 65 | 4 | 0 | 1 | 65 | 1 | 65 | 2.80E-24 | 120.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene031549.t1 | P46302 | 89.2 | 65 | 7 | 0 | 1 | 65 | 1 | 65 | 3.4e-23 | 108.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene031549.t1 | A0A1S2YMY4 | 93.8 | 65 | 4 | 0 | 1 | 65 | 1 | 65 | 2.0e-24 | 120.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene031549 | MS.gene50744 | PPI |
| MS.gene031549 | MS.gene072782 | PPI |
| MS.gene031549 | MS.gene87287 | PPI |
| MS.gene031549 | MS.gene83444 | PPI |
| MS.gene031549 | MS.gene72768 | PPI |
| MS.gene031549 | MS.gene031256 | PPI |
| MS.gene031549 | MS.gene87285 | PPI |
| MS.gene031549 | MS.gene96825 | PPI |
| MS.gene031549 | MS.gene003693 | PPI |
| MS.gene031549 | MS.gene019309 | PPI |
| MS.gene031549 | MS.gene99823 | PPI |
| MS.gene031549 | MS.gene23314 | PPI |
| MS.gene031549 | MS.gene006754 | PPI |
| MS.gene031549 | MS.gene86059 | PPI |
| MS.gene031549 | MS.gene82426 | PPI |
| MS.gene031549 | MS.gene031254 | PPI |
| MS.gene031549 | MS.gene69814 | PPI |
| MS.gene031549 | MS.gene028153 | PPI |
| MS.gene031549 | MS.gene015586 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene031549.t1 | MTR_4g081180 | 93.846 | 65 | 4 | 0 | 1 | 65 | 150 | 214 | 7.98e-38 | 124 |
| MS.gene031549.t1 | MTR_6g013210 | 90.769 | 65 | 6 | 0 | 1 | 65 | 1 | 65 | 1.60e-37 | 119 |
| MS.gene031549.t1 | MTR_6g013410 | 75.385 | 65 | 15 | 1 | 1 | 65 | 38 | 101 | 7.47e-28 | 96.3 |
| MS.gene031549.t1 | MTR_8g089765 | 70.000 | 60 | 18 | 0 | 1 | 60 | 1 | 60 | 2.25e-25 | 90.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene031549.t1 | AT5G03850 | 87.692 | 65 | 7 | 1 | 1 | 65 | 1 | 64 | 2.56e-34 | 111 |
| MS.gene031549.t1 | AT3G10090 | 87.692 | 65 | 7 | 1 | 1 | 65 | 1 | 64 | 2.56e-34 | 111 |
| MS.gene031549.t1 | AT5G64140 | 87.692 | 65 | 7 | 1 | 1 | 65 | 1 | 64 | 5.01e-34 | 110 |
Find 22 sgRNAs with CRISPR-Local
Find 22 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGGGTGTCTCCTTCTCTAAC+TGG | 0.224700 | 4.2:-28481827 | None:intergenic |
| TGCTGTTGTTGTCAAGGTTA+TGG | 0.305748 | 4.2:+28481715 | MS.gene031549:CDS |
| GCTGTTGTTGTCAAGGTTAT+GGG | 0.344081 | 4.2:+28481716 | MS.gene031549:CDS |
| GAGGACAGGTGACTCAAGTT+AGG | 0.354183 | 4.2:+28481753 | MS.gene031549:CDS |
| TTCCTCATGATGTGACGGTT+CGG | 0.361521 | 4.2:-28481794 | None:intergenic |
| TCCTCATGATGTGACGGTTC+GGG | 0.372907 | 4.2:-28481793 | None:intergenic |
| CTTCTCTTTCAGACTCAAGC+AGG | 0.393026 | 4.2:-28481853 | None:intergenic |
| GTCAAGGTTATGGGTCGTAC+TGG | 0.486004 | 4.2:+28481725 | MS.gene031549:CDS |
| TTTCAGACTCAAGCAGGGTG+AGG | 0.496062 | 4.2:-28481847 | None:intergenic |
| GGGTGTCTCCTTCTCTAACT+GGG | 0.522501 | 4.2:-28481826 | None:intergenic |
| AGGACAGGTGACTCAAGTTA+GGG | 0.535175 | 4.2:+28481754 | MS.gene031549:CDS |
| CAAGTATGCTGTTGTTGTCA+AGG | 0.536125 | 4.2:+28481709 | MS.gene031549:CDS |
| TCGTACTGGTTCTAGAGGAC+AGG | 0.538891 | 4.2:+28481739 | MS.gene031549:CDS |
| TTCAGACTCAAGCAGGGTGA+GGG | 0.547860 | 4.2:-28481846 | None:intergenic |
| ACCCGAACCGTCACATCATG+AGG | 0.555528 | 4.2:+28481792 | MS.gene031549:CDS |
| TTCTCTTTCAGACTCAAGCA+GGG | 0.585301 | 4.2:-28481852 | None:intergenic |
| TCACATCATGAGGAATGTGA+AGG | 0.587194 | 4.2:+28481802 | MS.gene031549:CDS |
| TCACATTCCTCATGATGTGA+CGG | 0.589714 | 4.2:-28481799 | None:intergenic |
| CACATCATGAGGAATGTGAA+GGG | 0.593653 | 4.2:+28481803 | MS.gene031549:CDS |
| GTGAAGGGCCCAGTTAGAGA+AGG | 0.598823 | 4.2:+28481818 | MS.gene031549:CDS |
| AGTCTGAAAGAGAAGCAAGA+AGG | 0.599710 | 4.2:+28481861 | MS.gene031549:CDS |
| ATGGGTCGTACTGGTTCTAG+AGG | 0.630812 | 4.2:+28481734 | MS.gene031549:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AGTCTGAAAGAGAAGCAAGA+AGG | + | chr4.2:28481861-28481880 | MS.gene031549:CDS | 40.0% | |
| CAAGTATGCTGTTGTTGTCA+AGG | + | chr4.2:28481709-28481728 | MS.gene031549:CDS | 40.0% | |
| CACATCATGAGGAATGTGAA+GGG | + | chr4.2:28481803-28481822 | MS.gene031549:CDS | 40.0% | |
| GCTGTTGTTGTCAAGGTTAT+GGG | + | chr4.2:28481716-28481735 | MS.gene031549:CDS | 40.0% | |
| TCACATCATGAGGAATGTGA+AGG | + | chr4.2:28481802-28481821 | MS.gene031549:CDS | 40.0% | |
| TCACATTCCTCATGATGTGA+CGG | - | chr4.2:28481802-28481821 | None:intergenic | 40.0% | |
| TGCTGTTGTTGTCAAGGTTA+TGG | + | chr4.2:28481715-28481734 | MS.gene031549:CDS | 40.0% | |
| TTCTCTTTCAGACTCAAGCA+GGG | - | chr4.2:28481855-28481874 | None:intergenic | 40.0% | |
| AGGACAGGTGACTCAAGTTA+GGG | + | chr4.2:28481754-28481773 | MS.gene031549:CDS | 45.0% | |
| CTTCTCTTTCAGACTCAAGC+AGG | - | chr4.2:28481856-28481875 | None:intergenic | 45.0% | |
| TTCCTCATGATGTGACGGTT+CGG | - | chr4.2:28481797-28481816 | None:intergenic | 45.0% | |
| GAGGACAGGTGACTCAAGTT+AGG | + | chr4.2:28481753-28481772 | MS.gene031549:CDS | 50.0% | |
| GGGTGTCTCCTTCTCTAACT+GGG | - | chr4.2:28481829-28481848 | None:intergenic | 50.0% | |
| GTCAAGGTTATGGGTCGTAC+TGG | + | chr4.2:28481725-28481744 | MS.gene031549:CDS | 50.0% | |
| TCCTCATGATGTGACGGTTC+GGG | - | chr4.2:28481796-28481815 | None:intergenic | 50.0% | |
| TTCAGACTCAAGCAGGGTGA+GGG | - | chr4.2:28481849-28481868 | None:intergenic | 50.0% | |
| TTTCAGACTCAAGCAGGGTG+AGG | - | chr4.2:28481850-28481869 | None:intergenic | 50.0% | |
| ! | AGGGTGTCTCCTTCTCTAAC+TGG | - | chr4.2:28481830-28481849 | None:intergenic | 50.0% |
| !! | ATGGGTCGTACTGGTTCTAG+AGG | + | chr4.2:28481734-28481753 | MS.gene031549:CDS | 50.0% |
| !! | TCGTACTGGTTCTAGAGGAC+AGG | + | chr4.2:28481739-28481758 | MS.gene031549:CDS | 50.0% |
| ACCCGAACCGTCACATCATG+AGG | + | chr4.2:28481792-28481811 | MS.gene031549:CDS | 55.0% | |
| GTGAAGGGCCCAGTTAGAGA+AGG | + | chr4.2:28481818-28481837 | MS.gene031549:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 28481695 | 28481892 | 28481695 | ID=MS.gene031549 |
| chr4.2 | mRNA | 28481695 | 28481892 | 28481695 | ID=MS.gene031549.t1;Parent=MS.gene031549 |
| chr4.2 | exon | 28481695 | 28481892 | 28481695 | ID=MS.gene031549.t1.exon1;Parent=MS.gene031549.t1 |
| chr4.2 | CDS | 28481695 | 28481892 | 28481695 | ID=cds.MS.gene031549.t1;Parent=MS.gene031549.t1 |
| Gene Sequence |
| Protein sequence |
