AlfalfaGEDB | MS.gene96825

M. sativa cultivar XinJiangDaYe / MS.gene96825

KEGG: K02979
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0019538 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576
PFAM: PF01200.21

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene96825.t1	XP_004507193.1	93.8	65	4	0	1	65	1	65	2.80E-24	120.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene96825.t1	P46302	89.2	65	7	0	1	65	1	65	3.4e-23	108.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene96825.t1	A0A1S2YMY4	93.8	65	4	0	1	65	1	65	2.0e-24	120.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049387	MS.gene96825	0.809316	2.01E-50	-1.69E-46
MS.gene050134	MS.gene96825	0.87448	6.54E-68	-1.69E-46
MS.gene050281	MS.gene96825	0.813918	2.01E-51	-1.69E-46
MS.gene050791	MS.gene96825	0.811353	7.30E-51	-1.69E-46
MS.gene051904	MS.gene96825	0.847324	1.24E-59	-1.69E-46
MS.gene052666	MS.gene96825	0.857637	1.42E-62	-1.69E-46
MS.gene053224	MS.gene96825	0.874881	4.78E-68	-1.69E-46
MS.gene057272	MS.gene96825	0.852155	5.56E-61	-1.69E-46
MS.gene057641	MS.gene96825	0.840368	9.07E-58	-1.69E-46
MS.gene057643	MS.gene96825	0.877156	7.87E-69	-1.69E-46
MS.gene060483	MS.gene96825	0.827721	1.34E-54	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene96825	MS.gene019309	PPI
MS.gene96825	MS.gene99823	PPI
MS.gene96825	MS.gene23314	PPI
MS.gene96825	MS.gene86059	PPI
MS.gene69814	MS.gene96825	PPI
MS.gene96825	MS.gene015586	PPI
MS.gene87285	MS.gene96825	PPI
MS.gene96825	MS.gene50744	PPI
MS.gene82426	MS.gene96825	PPI
MS.gene96825	MS.gene072782	PPI
MS.gene031549	MS.gene96825	PPI
MS.gene003693	MS.gene96825	PPI
MS.gene96825	MS.gene83444	PPI
MS.gene006754	MS.gene96825	PPI
MS.gene96825	MS.gene72768	PPI
MS.gene99823	MS.gene96825	PPI
MS.gene031254	MS.gene96825	PPI
MS.gene96825	MS.gene031256	PPI
MS.gene87287	MS.gene96825	PPI
MS.gene028153	MS.gene96825	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene96825.t1	MTR_4g081180	93.846	65	4	0	1	65	150	214	7.98e-38	124
MS.gene96825.t1	MTR_6g013210	90.769	65	6	0	1	65	1	65	1.60e-37	119
MS.gene96825.t1	MTR_6g013410	75.385	65	15	1	1	65	38	101	7.47e-28	96.3
MS.gene96825.t1	MTR_8g089765	70.000	60	18	0	1	60	1	60	2.25e-25	90.1

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene96825.t1	AT5G03850	87.692	65	7	1	1	65	1	64	2.56e-34	111
MS.gene96825.t1	AT3G10090	87.692	65	7	1	1	65	1	64	2.56e-34	111
MS.gene96825.t1	AT5G64140	87.692	65	7	1	1	65	1	64	5.01e-34	110

Find 22 sgRNAs with CRISPR-Local

Find 22 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGGGTGTCTCCTTCTCTAAC+TGG	0.224700	4.3:-28881747	None:intergenic
TGCTGTTGTTGTCAAGGTTA+TGG	0.305748	4.3:+28881635	MS.gene96825:CDS
GCTGTTGTTGTCAAGGTTAT+GGG	0.344081	4.3:+28881636	MS.gene96825:CDS
GAGGACAGGTGACTCAAGTT+AGG	0.354183	4.3:+28881673	MS.gene96825:CDS
TTCCTCATGATGTGACGGTT+CGG	0.361521	4.3:-28881714	None:intergenic
TCCTCATGATGTGACGGTTC+GGG	0.372907	4.3:-28881713	None:intergenic
CTTCTCTTTCAGACTCAAGC+AGG	0.393026	4.3:-28881773	None:intergenic
GTCAAGGTTATGGGTCGTAC+TGG	0.486004	4.3:+28881645	MS.gene96825:CDS
TTTCAGACTCAAGCAGGGTG+AGG	0.496062	4.3:-28881767	None:intergenic
GGGTGTCTCCTTCTCTAACT+GGG	0.522501	4.3:-28881746	None:intergenic
AGGACAGGTGACTCAAGTTA+GGG	0.535175	4.3:+28881674	MS.gene96825:CDS
CAAGTATGCTGTTGTTGTCA+AGG	0.536125	4.3:+28881629	MS.gene96825:CDS
TCGTACTGGTTCTAGAGGAC+AGG	0.538891	4.3:+28881659	MS.gene96825:CDS
TTCAGACTCAAGCAGGGTGA+GGG	0.547860	4.3:-28881766	None:intergenic
ACCCGAACCGTCACATCATG+AGG	0.555528	4.3:+28881712	MS.gene96825:CDS
TTCTCTTTCAGACTCAAGCA+GGG	0.585301	4.3:-28881772	None:intergenic
TCACATCATGAGGAATGTGA+AGG	0.587194	4.3:+28881722	MS.gene96825:CDS
TCACATTCCTCATGATGTGA+CGG	0.589714	4.3:-28881719	None:intergenic
CACATCATGAGGAATGTGAA+GGG	0.593653	4.3:+28881723	MS.gene96825:CDS
GTGAAGGGCCCAGTTAGAGA+AGG	0.598823	4.3:+28881738	MS.gene96825:CDS
AGTCTGAAAGAGAAGCAAGA+AGG	0.599710	4.3:+28881781	MS.gene96825:CDS
ATGGGTCGTACTGGTTCTAG+AGG	0.630812	4.3:+28881654	MS.gene96825:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AGTCTGAAAGAGAAGCAAGA+AGG	+	chr4.3:28881781-28881800	MS.gene96825:CDS	40.0%
	CAAGTATGCTGTTGTTGTCA+AGG	+	chr4.3:28881629-28881648	MS.gene96825:CDS	40.0%
	CACATCATGAGGAATGTGAA+GGG	+	chr4.3:28881723-28881742	MS.gene96825:CDS	40.0%
	GCTGTTGTTGTCAAGGTTAT+GGG	+	chr4.3:28881636-28881655	MS.gene96825:CDS	40.0%
	TCACATCATGAGGAATGTGA+AGG	+	chr4.3:28881722-28881741	MS.gene96825:CDS	40.0%
	TCACATTCCTCATGATGTGA+CGG	-	chr4.3:28881722-28881741	None:intergenic	40.0%
	TGCTGTTGTTGTCAAGGTTA+TGG	+	chr4.3:28881635-28881654	MS.gene96825:CDS	40.0%
	TTCTCTTTCAGACTCAAGCA+GGG	-	chr4.3:28881775-28881794	None:intergenic	40.0%
	AGGACAGGTGACTCAAGTTA+GGG	+	chr4.3:28881674-28881693	MS.gene96825:CDS	45.0%
	CTTCTCTTTCAGACTCAAGC+AGG	-	chr4.3:28881776-28881795	None:intergenic	45.0%
	TTCCTCATGATGTGACGGTT+CGG	-	chr4.3:28881717-28881736	None:intergenic	45.0%
	GAGGACAGGTGACTCAAGTT+AGG	+	chr4.3:28881673-28881692	MS.gene96825:CDS	50.0%
	GGGTGTCTCCTTCTCTAACT+GGG	-	chr4.3:28881749-28881768	None:intergenic	50.0%
	GTCAAGGTTATGGGTCGTAC+TGG	+	chr4.3:28881645-28881664	MS.gene96825:CDS	50.0%
	TCCTCATGATGTGACGGTTC+GGG	-	chr4.3:28881716-28881735	None:intergenic	50.0%
	TTCAGACTCAAGCAGGGTGA+GGG	-	chr4.3:28881769-28881788	None:intergenic	50.0%
	TTTCAGACTCAAGCAGGGTG+AGG	-	chr4.3:28881770-28881789	None:intergenic	50.0%
!	AGGGTGTCTCCTTCTCTAAC+TGG	-	chr4.3:28881750-28881769	None:intergenic	50.0%
!!	ATGGGTCGTACTGGTTCTAG+AGG	+	chr4.3:28881654-28881673	MS.gene96825:CDS	50.0%
!!	TCGTACTGGTTCTAGAGGAC+AGG	+	chr4.3:28881659-28881678	MS.gene96825:CDS	50.0%
	ACCCGAACCGTCACATCATG+AGG	+	chr4.3:28881712-28881731	MS.gene96825:CDS	55.0%
	GTGAAGGGCCCAGTTAGAGA+AGG	+	chr4.3:28881738-28881757	MS.gene96825:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.3	gene	28881615	28881812	28881615	ID=MS.gene96825
chr4.3	mRNA	28881615	28881812	28881615	ID=MS.gene96825.t1;Parent=MS.gene96825
chr4.3	exon	28881615	28881812	28881615	ID=MS.gene96825.t1.exon1;Parent=MS.gene96825.t1
chr4.3	CDS	28881615	28881812	28881615	ID=cds.MS.gene96825.t1;Parent=MS.gene96825.t1

Gene Sequence	>MS.gene96825 ATGGATTCTCAAGTCAAGTATGCTGTTGTTGTCAAGGTTATGGGTCGTACTGGTTCTAGAGGACAGGTGACTCAAGTTAGGGTCAAGTTTAATGACGACCCGAACCGTCACATCATGAGGAATGTGAAGGGCCCAGTTAGAGAAGGAGACACCCTCACCCTGCTTGAGTCTGAAAGAGAAGCAAGAAGGTTGCGCTAG
Protein sequence	>MS.gene96825.t1 MDSQVKYAVVVKVMGRTGSRGQVTQVRVKFNDDPNRHIMRNVKGPVREGDTLTLLESEREARRLR