AlfalfaGEDB | MS.gene87285

M. sativa cultivar XinJiangDaYe / MS.gene87285

KEGG: K02979
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0019538 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576
PFAM: PF01200.21

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene87285.t1	XP_004489433.1	100	65	0	0	1	65	1	65	3.00E-26	127.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene87285.t1	P46302	90.8	65	6	0	1	65	1	65	1.4e-24	112.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene87285.t1	Q2HWC2	100.0	65	0	0	1	65	1	65	2.1e-26	127.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049255	MS.gene87285	0.826975	2.02E-54	-1.69E-46
MS.gene049341	MS.gene87285	0.811568	6.56E-51	-1.69E-46
MS.gene049666	MS.gene87285	0.801914	7.19E-49	-1.69E-46
MS.gene049825	MS.gene87285	0.824627	7.28E-54	-1.69E-46
MS.gene049873	MS.gene87285	0.826316	2.90E-54	-1.69E-46
MS.gene050277	MS.gene87285	0.83446	2.96E-56	-1.69E-46
MS.gene050303	MS.gene87285	0.874451	6.68E-68	-1.69E-46
MS.gene050815	MS.gene87285	0.810405	1.17E-50	-1.69E-46
MS.gene051241	MS.gene87285	0.804308	2.30E-49	-1.69E-46
MS.gene051243	MS.gene87285	0.806241	9.05E-50	-1.69E-46
MS.gene051247	MS.gene87285	0.806934	6.46E-50	-1.69E-46
MS.gene051328	MS.gene87285	0.825077	5.70E-54	-1.69E-46
MS.gene05134	MS.gene87285	0.851481	8.63E-61	-1.69E-46
MS.gene051451	MS.gene87285	0.817889	2.61E-52	-1.69E-46
MS.gene051633	MS.gene87285	0.814022	1.90E-51	-1.69E-46
MS.gene051700	MS.gene87285	0.81221	4.75E-51	-1.69E-46
MS.gene05188	MS.gene87285	0.814817	1.27E-51	-1.69E-46
MS.gene052302	MS.gene87285	0.806219	9.15E-50	-1.69E-46
MS.gene052376	MS.gene87285	0.829869	4.04E-55	-1.69E-46
MS.gene052519	MS.gene87285	0.820116	8.13E-53	-1.69E-46
MS.gene052947	MS.gene87285	0.834076	3.69E-56	-1.69E-46
MS.gene053006	MS.gene87285	0.829821	4.15E-55	-1.69E-46
MS.gene053226	MS.gene87285	0.813728	2.21E-51	-1.69E-46
MS.gene053294	MS.gene87285	0.814437	1.54E-51	-1.69E-46
MS.gene05365	MS.gene87285	0.807366	5.23E-50	-1.69E-46
MS.gene055162	MS.gene87285	0.821121	4.78E-53	-1.69E-46
MS.gene055778	MS.gene87285	0.840692	7.46E-58	-1.69E-46
MS.gene055808	MS.gene87285	0.851308	9.66E-61	-1.69E-46
MS.gene055818	MS.gene87285	0.802362	5.82E-49	-1.69E-46
MS.gene056091	MS.gene87285	0.849638	2.84E-60	-1.69E-46
MS.gene056215	MS.gene87285	0.847357	1.22E-59	-1.69E-46
MS.gene056498	MS.gene87285	0.80685	6.73E-50	-1.69E-46
MS.gene056601	MS.gene87285	0.800032	1.74E-48	-1.69E-46
MS.gene056631	MS.gene87285	0.862474	4.94E-64	-1.69E-46
MS.gene057060	MS.gene87285	0.815706	8.06E-52	-1.69E-46
MS.gene057079	MS.gene87285	0.825348	4.92E-54	-1.69E-46
MS.gene057140	MS.gene87285	0.801259	9.80E-49	-1.69E-46
MS.gene057555	MS.gene87285	0.821328	4.28E-53	-1.69E-46
MS.gene057693	MS.gene87285	0.85468	1.05E-61	-1.69E-46
MS.gene057705	MS.gene87285	0.839965	1.16E-57	-1.69E-46
MS.gene058037	MS.gene87285	0.828952	6.75E-55	-1.69E-46
MS.gene058084	MS.gene87285	0.833551	5.00E-56	-1.69E-46
MS.gene058238	MS.gene87285	0.801098	1.06E-48	-1.69E-46
MS.gene058548	MS.gene87285	0.807848	4.14E-50	-1.69E-46
MS.gene058549	MS.gene87285	0.819222	1.30E-52	-1.69E-46
MS.gene058906	MS.gene87285	0.826553	2.55E-54	-1.69E-46
MS.gene059249	MS.gene87285	0.810342	1.21E-50	-1.69E-46
MS.gene059317	MS.gene87285	0.813555	2.41E-51	-1.69E-46
MS.gene05953	MS.gene87285	0.810513	1.11E-50	-1.69E-46
MS.gene05954	MS.gene87285	0.856715	2.67E-62	-1.69E-46
MS.gene05979	MS.gene87285	0.813848	2.08E-51	-1.69E-46
MS.gene059830	MS.gene87285	0.829992	3.77E-55	-1.69E-46
MS.gene059842	MS.gene87285	0.822906	1.84E-53	-1.69E-46
MS.gene06001	MS.gene87285	0.854358	1.30E-61	-1.69E-46
MS.gene060429	MS.gene87285	0.893904	4.07E-75	-1.69E-46
MS.gene060586	MS.gene87285	0.832456	9.36E-56	-1.69E-46
MS.gene060907	MS.gene87285	0.808568	2.90E-50	-1.69E-46
MS.gene060917	MS.gene87285	0.848411	6.23E-60	-1.69E-46
MS.gene061040	MS.gene87285	-0.823905	1.08E-53	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene87285	MS.gene72768	PPI
MS.gene87285	MS.gene031256	PPI
MS.gene031254	MS.gene87285	PPI
MS.gene87285	MS.gene96825	PPI
MS.gene87285	MS.gene003693	PPI
MS.gene031549	MS.gene87285	PPI
MS.gene87285	MS.gene23314	PPI
MS.gene87285	MS.gene82426	PPI
MS.gene87285	MS.gene019309	PPI
MS.gene87285	MS.gene99823	PPI
MS.gene87285	MS.gene006754	PPI
MS.gene87285	MS.gene86059	PPI
MS.gene87285	MS.gene87287	PPI
MS.gene87285	MS.gene031254	PPI
MS.gene87285	MS.gene69814	PPI
MS.gene87285	MS.gene028153	PPI
MS.gene87285	MS.gene83444	PPI
MS.gene87285	MS.gene015586	PPI
MS.gene87285	MS.gene50744	PPI
MS.gene87285	MS.gene072782	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene87285.t1	MTR_6g013210	100.000	65	0	0	1	65	1	65	8.41e-42	130
MS.gene87285.t1	MTR_4g081180	90.769	65	6	0	1	65	150	214	8.23e-36	119
MS.gene87285.t1	MTR_6g013410	81.538	65	11	1	1	65	38	101	4.19e-31	104
MS.gene87285.t1	MTR_8g089765	71.667	60	17	0	1	60	1	60	5.17e-25	89.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene87285.t1	AT5G03850	90.769	65	5	1	1	65	1	64	1.28e-35	114
MS.gene87285.t1	AT3G10090	90.769	65	5	1	1	65	1	64	1.28e-35	114
MS.gene87285.t1	AT5G64140	89.231	65	6	1	1	65	1	64	1.09e-34	112

Find 20 sgRNAs with CRISPR-Local

Find 21 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GCTGTTGTTGTGAAAGTTAT+GGG	0.420809	2.2:+14203576	MS.gene87285:CDS
AGGATGTCTCCCTCCCTAAC+TGG	0.425936	2.2:-14203687	None:intergenic
TGCTGTTGTTGTGAAAGTTA+TGG	0.441002	2.2:+14203575	MS.gene87285:CDS
TTATGGGTCGTACTGGATCC+AGG	0.451262	2.2:+14203592	MS.gene87285:CDS
AACCTGAGTGACTTGTCCCC+TGG	0.461687	2.2:-14203610	None:intergenic
GAATGTGAAGGGACCAGTTA+GGG	0.464736	2.2:+14203674	MS.gene87285:CDS
GGAATGTGAAGGGACCAGTT+AGG	0.473428	2.2:+14203673	MS.gene87285:CDS
TCTCAGACTCGAGGAGGGTA+AGG	0.488938	2.2:-14203707	None:intergenic
GTGAAAGTTATGGGTCGTAC+TGG	0.530087	2.2:+14203585	MS.gene87285:CDS
TGTGAAGGGACCAGTTAGGG+AGG	0.582336	2.2:+14203677	MS.gene87285:CDS
ATCAGAATCGTCACATCATG+AGG	0.584464	2.2:+14203652	MS.gene87285:CDS
TTGCTTCTCTCTCAGACTCG+AGG	0.586554	2.2:-14203716	None:intergenic
CTTCTCTCTCAGACTCGAGG+AGG	0.606831	2.2:-14203713	None:intergenic
TATGGGTCGTACTGGATCCA+GGG	0.613108	2.2:+14203593	MS.gene87285:CDS
TCACATCATGAGGAATGTGA+AGG	0.626309	2.2:+14203662	MS.gene87285:CDS
CACATCATGAGGAATGTGAA+GGG	0.632733	2.2:+14203663	MS.gene87285:CDS
ATCCAGGGGACAAGTCACTC+AGG	0.638739	2.2:+14203608	MS.gene87285:CDS
TTCTCTCTCAGACTCGAGGA+GGG	0.644799	2.2:-14203712	None:intergenic
GTGAAGGGACCAGTTAGGGA+GGG	0.647685	2.2:+14203678	MS.gene87285:CDS
ATGGGTCGTACTGGATCCAG+GGG	0.674814	2.2:+14203594	MS.gene87285:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	GCTGTTGTTGTGAAAGTTAT+GGG	+	chr2.2:14203576-14203595	MS.gene87285:CDS	35.0%
	TGCTGTTGTTGTGAAAGTTA+TGG	+	chr2.2:14203575-14203594	MS.gene87285:CDS	35.0%
!	TCAGGTTAGAGTGAAGTTTT+TGG	+	chr2.2:14203626-14203645	MS.gene87285:CDS	35.0%
	ATCAGAATCGTCACATCATG+AGG	+	chr2.2:14203652-14203671	MS.gene87285:CDS	40.0%
	CACATCATGAGGAATGTGAA+GGG	+	chr2.2:14203663-14203682	MS.gene87285:CDS	40.0%
	TCACATCATGAGGAATGTGA+AGG	+	chr2.2:14203662-14203681	MS.gene87285:CDS	40.0%
	GAATGTGAAGGGACCAGTTA+GGG	+	chr2.2:14203674-14203693	MS.gene87285:CDS	45.0%
	GTGAAAGTTATGGGTCGTAC+TGG	+	chr2.2:14203585-14203604	MS.gene87285:CDS	45.0%
	GGAATGTGAAGGGACCAGTT+AGG	+	chr2.2:14203673-14203692	MS.gene87285:CDS	50.0%
	TATGGGTCGTACTGGATCCA+GGG	+	chr2.2:14203593-14203612	MS.gene87285:CDS	50.0%
	TTATGGGTCGTACTGGATCC+AGG	+	chr2.2:14203592-14203611	MS.gene87285:CDS	50.0%
	TTCTCTCTCAGACTCGAGGA+GGG	-	chr2.2:14203715-14203734	None:intergenic	50.0%
	TTGCTTCTCTCTCAGACTCG+AGG	-	chr2.2:14203719-14203738	None:intergenic	50.0%
	AGGATGTCTCCCTCCCTAAC+TGG	-	chr2.2:14203690-14203709	None:intergenic	55.0%
	ATCCAGGGGACAAGTCACTC+AGG	+	chr2.2:14203608-14203627	MS.gene87285:CDS	55.0%
	ATGGGTCGTACTGGATCCAG+GGG	+	chr2.2:14203594-14203613	MS.gene87285:CDS	55.0%
	CTTCTCTCTCAGACTCGAGG+AGG	-	chr2.2:14203716-14203735	None:intergenic	55.0%
	GTGAAGGGACCAGTTAGGGA+GGG	+	chr2.2:14203678-14203697	MS.gene87285:CDS	55.0%
	TCTCAGACTCGAGGAGGGTA+AGG	-	chr2.2:14203710-14203729	None:intergenic	55.0%
	TGTGAAGGGACCAGTTAGGG+AGG	+	chr2.2:14203677-14203696	MS.gene87285:CDS	55.0%
!	AACCTGAGTGACTTGTCCCC+TGG	-	chr2.2:14203613-14203632	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.2	gene	14203555	14203752	14203555	ID=MS.gene87285
chr2.2	mRNA	14203555	14203752	14203555	ID=MS.gene87285.t1;Parent=MS.gene87285
chr2.2	exon	14203555	14203752	14203555	ID=MS.gene87285.t1.exon1;Parent=MS.gene87285.t1
chr2.2	CDS	14203555	14203752	14203555	ID=cds.MS.gene87285.t1;Parent=MS.gene87285.t1

Gene Sequence	>MS.gene87285 ATGGAATCACAGATTAAGCATGCTGTTGTTGTGAAAGTTATGGGTCGTACTGGATCCAGGGGACAAGTCACTCAGGTTAGAGTGAAGTTTTTGGATGATCAGAATCGTCACATCATGAGGAATGTGAAGGGACCAGTTAGGGAGGGAGACATCCTTACCCTCCTCGAGTCTGAGAGAGAAGCAAGAAGACTCCGATAG
Protein sequence	>MS.gene87285.t1 MESQIKHAVVVKVMGRTGSRGQVTQVRVKFLDDQNRHIMRNVKGPVREGDILTLLESEREARRLR