AlfalfaGEDB | MS.gene99823

M. sativa cultivar XinJiangDaYe / MS.gene99823

KEGG: K02979
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0019538 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576
PFAM: PF01200.21

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene99823.t1	XP_004489433.1	100	65	0	0	1	65	1	65	3.00E-26	127.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene99823.t1	P46302	90.8	65	6	0	1	65	1	65	1.4e-24	112.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene99823.t1	Q2HWC2	100.0	65	0	0	1	65	1	65	2.1e-26	127.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene056257	MS.gene99823	0.825156	5.47E-54	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene96825	MS.gene99823	PPI
MS.gene99823	MS.gene86059	PPI
MS.gene99823	MS.gene83444	PPI
MS.gene82426	MS.gene99823	PPI
MS.gene99823	MS.gene50744	PPI
MS.gene99823	MS.gene031256	PPI
MS.gene99823	MS.gene072782	PPI
MS.gene69814	MS.gene99823	PPI
MS.gene99823	MS.gene72768	PPI
MS.gene87285	MS.gene99823	PPI
MS.gene99823	MS.gene96825	PPI
MS.gene031254	MS.gene99823	PPI
MS.gene031549	MS.gene99823	PPI
MS.gene003693	MS.gene99823	PPI
MS.gene99823	MS.gene23314	PPI
MS.gene006754	MS.gene99823	PPI
MS.gene99823	MS.gene015586	PPI
MS.gene99823	MS.gene019309	PPI
MS.gene028153	MS.gene99823	PPI
MS.gene87287	MS.gene99823	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene99823.t1	MTR_6g013210	100.000	65	0	0	1	65	1	65	8.41e-42	130
MS.gene99823.t1	MTR_4g081180	90.769	65	6	0	1	65	150	214	8.23e-36	119
MS.gene99823.t1	MTR_6g013410	81.538	65	11	1	1	65	38	101	4.19e-31	104
MS.gene99823.t1	MTR_8g089765	71.667	60	17	0	1	60	1	60	5.17e-25	89.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene99823.t1	AT5G03850	90.769	65	5	1	1	65	1	64	1.28e-35	114
MS.gene99823.t1	AT3G10090	90.769	65	5	1	1	65	1	64	1.28e-35	114
MS.gene99823.t1	AT5G64140	89.231	65	6	1	1	65	1	64	1.09e-34	112

Find 20 sgRNAs with CRISPR-Local

Find 21 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GCTGTTGTTGTGAAAGTTAT+GGG	0.420809	2.3:+14587224	MS.gene99823:CDS
AGGATGTCTCCCTCCCTAAC+TGG	0.425936	2.3:-14587335	None:intergenic
TGCTGTTGTTGTGAAAGTTA+TGG	0.441002	2.3:+14587223	MS.gene99823:CDS
TTATGGGTCGTACTGGATCC+AGG	0.451262	2.3:+14587240	MS.gene99823:CDS
AACCTGAGTGACTTGTCCCC+TGG	0.461687	2.3:-14587258	None:intergenic
GAATGTGAAGGGACCAGTTA+GGG	0.464736	2.3:+14587322	MS.gene99823:CDS
GGAATGTGAAGGGACCAGTT+AGG	0.473428	2.3:+14587321	MS.gene99823:CDS
TCTCAGACTCGAGGAGGGTA+AGG	0.488938	2.3:-14587355	None:intergenic
GTGAAAGTTATGGGTCGTAC+TGG	0.530087	2.3:+14587233	MS.gene99823:CDS
TGTGAAGGGACCAGTTAGGG+AGG	0.582336	2.3:+14587325	MS.gene99823:CDS
ATCAGAATCGTCACATCATG+AGG	0.584464	2.3:+14587300	MS.gene99823:CDS
TTGCTTCTCTCTCAGACTCG+AGG	0.586554	2.3:-14587364	None:intergenic
CTTCTCTCTCAGACTCGAGG+AGG	0.606831	2.3:-14587361	None:intergenic
TATGGGTCGTACTGGATCCA+GGG	0.613108	2.3:+14587241	MS.gene99823:CDS
TCACATCATGAGGAATGTGA+AGG	0.626309	2.3:+14587310	MS.gene99823:CDS
CACATCATGAGGAATGTGAA+GGG	0.632733	2.3:+14587311	MS.gene99823:CDS
ATCCAGGGGACAAGTCACTC+AGG	0.638739	2.3:+14587256	MS.gene99823:CDS
TTCTCTCTCAGACTCGAGGA+GGG	0.644799	2.3:-14587360	None:intergenic
GTGAAGGGACCAGTTAGGGA+GGG	0.647685	2.3:+14587326	MS.gene99823:CDS
ATGGGTCGTACTGGATCCAG+GGG	0.674814	2.3:+14587242	MS.gene99823:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	GCTGTTGTTGTGAAAGTTAT+GGG	+	chr2.3:14587224-14587243	MS.gene99823:CDS	35.0%
	TGCTGTTGTTGTGAAAGTTA+TGG	+	chr2.3:14587223-14587242	MS.gene99823:CDS	35.0%
!	TCAGGTTAGAGTGAAGTTTT+TGG	+	chr2.3:14587274-14587293	MS.gene99823:CDS	35.0%
	ATCAGAATCGTCACATCATG+AGG	+	chr2.3:14587300-14587319	MS.gene99823:CDS	40.0%
	CACATCATGAGGAATGTGAA+GGG	+	chr2.3:14587311-14587330	MS.gene99823:CDS	40.0%
	TCACATCATGAGGAATGTGA+AGG	+	chr2.3:14587310-14587329	MS.gene99823:CDS	40.0%
	GAATGTGAAGGGACCAGTTA+GGG	+	chr2.3:14587322-14587341	MS.gene99823:CDS	45.0%
	GTGAAAGTTATGGGTCGTAC+TGG	+	chr2.3:14587233-14587252	MS.gene99823:CDS	45.0%
	GGAATGTGAAGGGACCAGTT+AGG	+	chr2.3:14587321-14587340	MS.gene99823:CDS	50.0%
	TATGGGTCGTACTGGATCCA+GGG	+	chr2.3:14587241-14587260	MS.gene99823:CDS	50.0%
	TTATGGGTCGTACTGGATCC+AGG	+	chr2.3:14587240-14587259	MS.gene99823:CDS	50.0%
	TTCTCTCTCAGACTCGAGGA+GGG	-	chr2.3:14587363-14587382	None:intergenic	50.0%
	TTGCTTCTCTCTCAGACTCG+AGG	-	chr2.3:14587367-14587386	None:intergenic	50.0%
	AGGATGTCTCCCTCCCTAAC+TGG	-	chr2.3:14587338-14587357	None:intergenic	55.0%
	ATCCAGGGGACAAGTCACTC+AGG	+	chr2.3:14587256-14587275	MS.gene99823:CDS	55.0%
	ATGGGTCGTACTGGATCCAG+GGG	+	chr2.3:14587242-14587261	MS.gene99823:CDS	55.0%
	CTTCTCTCTCAGACTCGAGG+AGG	-	chr2.3:14587364-14587383	None:intergenic	55.0%
	GTGAAGGGACCAGTTAGGGA+GGG	+	chr2.3:14587326-14587345	MS.gene99823:CDS	55.0%
	TCTCAGACTCGAGGAGGGTA+AGG	-	chr2.3:14587358-14587377	None:intergenic	55.0%
	TGTGAAGGGACCAGTTAGGG+AGG	+	chr2.3:14587325-14587344	MS.gene99823:CDS	55.0%
!	AACCTGAGTGACTTGTCCCC+TGG	-	chr2.3:14587261-14587280	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.3	gene	14587203	14587400	14587203	ID=MS.gene99823
chr2.3	mRNA	14587203	14587400	14587203	ID=MS.gene99823.t1;Parent=MS.gene99823
chr2.3	exon	14587203	14587400	14587203	ID=MS.gene99823.t1.exon1;Parent=MS.gene99823.t1
chr2.3	CDS	14587203	14587400	14587203	ID=cds.MS.gene99823.t1;Parent=MS.gene99823.t1

Gene Sequence	>MS.gene99823 ATGGAATCACAGATTAAGCATGCTGTTGTTGTGAAAGTTATGGGTCGTACTGGATCCAGGGGACAAGTCACTCAGGTTAGAGTGAAGTTTTTGGATGATCAGAATCGTCACATCATGAGGAATGTGAAGGGACCAGTTAGGGAGGGAGACATCCTTACCCTCCTCGAGTCTGAGAGAGAAGCAAGAAGACTCCGATAG
Protein sequence	>MS.gene99823.t1 MESQIKHAVVVKVMGRTGSRGQVTQVRVKFLDDQNRHIMRNVKGPVREGDILTLLESEREARRLR