AlfalfaGEDB | MS.gene87287

M. sativa cultivar XinJiangDaYe / MS.gene87287

KEGG: K02979
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0019538 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576
PFAM: PF01200.21

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene87287.t1	XP_004489433.1	93.8	65	4	0	1	65	1	65	4.80E-24	119.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene87287.t1	P46302	84.6	65	10	0	1	65	1	65	2.2e-22	105.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene87287.t1	Q2HWC2	93.8	65	4	0	1	65	1	65	3.4e-24	119.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene031254	MS.gene87287	PPI
MS.gene031549	MS.gene87287	PPI
MS.gene87287	MS.gene006754	PPI
MS.gene87287	MS.gene86059	PPI
MS.gene87287	MS.gene69814	PPI
MS.gene87287	MS.gene028153	PPI
MS.gene87287	MS.gene83444	PPI
MS.gene87287	MS.gene015586	PPI
MS.gene87287	MS.gene50744	PPI
MS.gene87287	MS.gene072782	PPI
MS.gene87287	MS.gene72768	PPI
MS.gene87287	MS.gene031256	PPI
MS.gene87285	MS.gene87287	PPI
MS.gene87287	MS.gene96825	PPI
MS.gene87287	MS.gene003693	PPI
MS.gene87287	MS.gene23314	PPI
MS.gene87287	MS.gene82426	PPI
MS.gene87287	MS.gene019309	PPI
MS.gene87287	MS.gene99823	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene87287.t1	MTR_6g013210	93.846	65	4	0	1	65	1	65	1.58e-38	122
MS.gene87287.t1	MTR_4g081180	86.154	65	9	0	1	65	150	214	4.73e-33	112
MS.gene87287.t1	MTR_6g013410	78.462	65	13	1	1	65	38	101	5.74e-28	96.3
MS.gene87287.t1	MTR_8g089765	81.633	49	9	0	1	49	1	49	2.67e-24	87.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene87287.t1	AT5G03850	84.615	65	9	1	1	65	1	64	2.98e-32	106
MS.gene87287.t1	AT3G10090	84.615	65	9	1	1	65	1	64	2.98e-32	106
MS.gene87287.t1	AT5G64140	86.154	65	8	1	1	65	1	64	7.17e-32	105

Find 17 sgRNAs with CRISPR-Local

Find 18 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GTTGTTGTTGTGAAAGTTAT+GGG	0.376114	2.2:+14199319	MS.gene87287:CDS
AGAACGTATCCCTCCCTAAC+TGG	0.425380	2.2:-14199430	None:intergenic
TGTTGTTGTTGTGAAAGTTA+TGG	0.451434	2.2:+14199318	MS.gene87287:CDS
GAATGTGAAGGGACCAGTTA+GGG	0.464736	2.2:+14199417	MS.gene87287:CDS
AACCTGAGTGACTTGTCCTC+TGG	0.467654	2.2:-14199353	None:intergenic
GGAATGTGAAGGGACCAGTT+AGG	0.473428	2.2:+14199416	MS.gene87287:CDS
GTGAAAGTTATGGGTCGTAC+TGG	0.530087	2.2:+14199328	MS.gene87287:CDS
TTGCTTCTCTCTCAAACTCA+AGG	0.538040	2.2:-14199459	None:intergenic
TGTGAAGGGACCAGTTAGGG+AGG	0.582336	2.2:+14199420	MS.gene87287:CDS
ATCAGAATCGTCACATCATG+AGG	0.585221	2.2:+14199395	MS.gene87287:CDS
CTTCTCTCTCAAACTCAAGG+AGG	0.613685	2.2:-14199456	None:intergenic
TTCTCTCTCAAACTCAAGGA+GGG	0.614914	2.2:-14199455	None:intergenic
TCACATCATGAGGAATGTGA+AGG	0.626309	2.2:+14199405	MS.gene87287:CDS
CACATCATGAGGAATGTGAA+GGG	0.632733	2.2:+14199406	MS.gene87287:CDS
ATCCAGAGGACAAGTCACTC+AGG	0.638739	2.2:+14199351	MS.gene87287:CDS
GTGAAGGGACCAGTTAGGGA+GGG	0.657315	2.2:+14199421	MS.gene87287:CDS
ATGGGTCGTACTGGATCCAG+AGG	0.663896	2.2:+14199337	MS.gene87287:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	GTTGTTGTTGTGAAAGTTAT+GGG	+	chr2.2:14199319-14199338	MS.gene87287:CDS	30.0%
!	TGTTGTTGTTGTGAAAGTTA+TGG	+	chr2.2:14199318-14199337	MS.gene87287:CDS	30.0%
!	TCAGGTTAGAGTGAAGTTTT+TGG	+	chr2.2:14199369-14199388	MS.gene87287:CDS	35.0%
	ATCAGAATCGTCACATCATG+AGG	+	chr2.2:14199395-14199414	MS.gene87287:CDS	40.0%
	CACATCATGAGGAATGTGAA+GGG	+	chr2.2:14199406-14199425	MS.gene87287:CDS	40.0%
	TCACATCATGAGGAATGTGA+AGG	+	chr2.2:14199405-14199424	MS.gene87287:CDS	40.0%
	TTCTCTCTCAAACTCAAGGA+GGG	-	chr2.2:14199458-14199477	None:intergenic	40.0%
	TTGCTTCTCTCTCAAACTCA+AGG	-	chr2.2:14199462-14199481	None:intergenic	40.0%
	CTTCTCTCTCAAACTCAAGG+AGG	-	chr2.2:14199459-14199478	None:intergenic	45.0%
	GAATGTGAAGGGACCAGTTA+GGG	+	chr2.2:14199417-14199436	MS.gene87287:CDS	45.0%
	GTGAAAGTTATGGGTCGTAC+TGG	+	chr2.2:14199328-14199347	MS.gene87287:CDS	45.0%
	AGAACGTATCCCTCCCTAAC+TGG	-	chr2.2:14199433-14199452	None:intergenic	50.0%
	ATCCAGAGGACAAGTCACTC+AGG	+	chr2.2:14199351-14199370	MS.gene87287:CDS	50.0%
	GGAATGTGAAGGGACCAGTT+AGG	+	chr2.2:14199416-14199435	MS.gene87287:CDS	50.0%
!	AACCTGAGTGACTTGTCCTC+TGG	-	chr2.2:14199356-14199375	None:intergenic	50.0%
	ATGGGTCGTACTGGATCCAG+AGG	+	chr2.2:14199337-14199356	MS.gene87287:CDS	55.0%
	GTGAAGGGACCAGTTAGGGA+GGG	+	chr2.2:14199421-14199440	MS.gene87287:CDS	55.0%
	TGTGAAGGGACCAGTTAGGG+AGG	+	chr2.2:14199420-14199439	MS.gene87287:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.2	gene	14199298	14199495	14199298	ID=MS.gene87287
chr2.2	mRNA	14199298	14199495	14199298	ID=MS.gene87287.t1;Parent=MS.gene87287
chr2.2	exon	14199298	14199495	14199298	ID=MS.gene87287.t1.exon1;Parent=MS.gene87287.t1
chr2.2	CDS	14199298	14199495	14199298	ID=cds.MS.gene87287.t1;Parent=MS.gene87287.t1

Gene Sequence	>MS.gene87287 ATGGAATCACAGATTAAGCATGTTGTTGTTGTGAAAGTTATGGGTCGTACTGGATCCAGAGGACAAGTCACTCAGGTTAGAGTGAAGTTTTTGGACGATCAGAATCGTCACATCATGAGGAATGTGAAGGGACCAGTTAGGGAGGGATACGTTCTCACCCTCCTTGAGTTTGAGAGAGAAGCAAGAAGACTCCGATAG
Protein sequence	>MS.gene87287.t1 MESQIKHVVVVKVMGRTGSRGQVTQVRVKFLDDQNRHIMRNVKGPVREGYVLTLLEFEREARRLR

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