AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene031256


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene031256.t1 MTR_6g013210 100.000 65 0 0 1 65 1 65 8.41e-42 130
MS.gene031256.t1 MTR_4g081180 90.769 65 6 0 1 65 150 214 8.23e-36 119
MS.gene031256.t1 MTR_6g013410 81.538 65 11 1 1 65 38 101 4.19e-31 104
MS.gene031256.t1 MTR_8g089765 71.667 60 17 0 1 60 1 60 5.17e-25 89.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene031256.t1 AT5G03850 90.769 65 5 1 1 65 1 64 1.28e-35 114
MS.gene031256.t1 AT3G10090 90.769 65 5 1 1 65 1 64 1.28e-35 114
MS.gene031256.t1 AT5G64140 89.231 65 6 1 1 65 1 64 1.09e-34 112

Find 20 sgRNAs with CRISPR-Local

Find 21 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTGTTGTTGTGAAAGTTAT+GGG 0.420809 2.1:+18068713 MS.gene031256:CDS
AGGATGTCTCCCTCCCTAAC+TGG 0.425936 2.1:-18068824 None:intergenic
TGCTGTTGTTGTGAAAGTTA+TGG 0.441002 2.1:+18068712 MS.gene031256:CDS
TTATGGGTCGTACTGGATCC+AGG 0.451262 2.1:+18068729 MS.gene031256:CDS
AACCTGAGTGACTTGTCCCC+TGG 0.461687 2.1:-18068747 None:intergenic
GAATGTGAAGGGACCAGTTA+GGG 0.464736 2.1:+18068811 MS.gene031256:CDS
GGAATGTGAAGGGACCAGTT+AGG 0.473428 2.1:+18068810 MS.gene031256:CDS
TCTCAGACTCGAGGAGGGTA+AGG 0.488938 2.1:-18068844 None:intergenic
GTGAAAGTTATGGGTCGTAC+TGG 0.530087 2.1:+18068722 MS.gene031256:CDS
TGTGAAGGGACCAGTTAGGG+AGG 0.582336 2.1:+18068814 MS.gene031256:CDS
ATCAGAATCGTCACATCATG+AGG 0.584464 2.1:+18068789 MS.gene031256:CDS
TTGCTTCTCTCTCAGACTCG+AGG 0.586554 2.1:-18068853 None:intergenic
CTTCTCTCTCAGACTCGAGG+AGG 0.606831 2.1:-18068850 None:intergenic
TATGGGTCGTACTGGATCCA+GGG 0.613108 2.1:+18068730 MS.gene031256:CDS
TCACATCATGAGGAATGTGA+AGG 0.626309 2.1:+18068799 MS.gene031256:CDS
CACATCATGAGGAATGTGAA+GGG 0.632733 2.1:+18068800 MS.gene031256:CDS
ATCCAGGGGACAAGTCACTC+AGG 0.638739 2.1:+18068745 MS.gene031256:CDS
TTCTCTCTCAGACTCGAGGA+GGG 0.644799 2.1:-18068849 None:intergenic
GTGAAGGGACCAGTTAGGGA+GGG 0.647685 2.1:+18068815 MS.gene031256:CDS
ATGGGTCGTACTGGATCCAG+GGG 0.674814 2.1:+18068731 MS.gene031256:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
GCTGTTGTTGTGAAAGTTAT+GGG + chr2.1:18068713-18068732 MS.gene031256:CDS 35.0%
TGCTGTTGTTGTGAAAGTTA+TGG + chr2.1:18068712-18068731 MS.gene031256:CDS 35.0%
! TCAGGTTAGAGTGAAGTTTT+TGG + chr2.1:18068763-18068782 MS.gene031256:CDS 35.0%
ATCAGAATCGTCACATCATG+AGG + chr2.1:18068789-18068808 MS.gene031256:CDS 40.0%
CACATCATGAGGAATGTGAA+GGG + chr2.1:18068800-18068819 MS.gene031256:CDS 40.0%
TCACATCATGAGGAATGTGA+AGG + chr2.1:18068799-18068818 MS.gene031256:CDS 40.0%
GAATGTGAAGGGACCAGTTA+GGG + chr2.1:18068811-18068830 MS.gene031256:CDS 45.0%
GTGAAAGTTATGGGTCGTAC+TGG + chr2.1:18068722-18068741 MS.gene031256:CDS 45.0%
GGAATGTGAAGGGACCAGTT+AGG + chr2.1:18068810-18068829 MS.gene031256:CDS 50.0%
TATGGGTCGTACTGGATCCA+GGG + chr2.1:18068730-18068749 MS.gene031256:CDS 50.0%
TTATGGGTCGTACTGGATCC+AGG + chr2.1:18068729-18068748 MS.gene031256:CDS 50.0%
TTCTCTCTCAGACTCGAGGA+GGG - chr2.1:18068852-18068871 None:intergenic 50.0%
TTGCTTCTCTCTCAGACTCG+AGG - chr2.1:18068856-18068875 None:intergenic 50.0%
AGGATGTCTCCCTCCCTAAC+TGG - chr2.1:18068827-18068846 None:intergenic 55.0%
ATCCAGGGGACAAGTCACTC+AGG + chr2.1:18068745-18068764 MS.gene031256:CDS 55.0%
ATGGGTCGTACTGGATCCAG+GGG + chr2.1:18068731-18068750 MS.gene031256:CDS 55.0%
CTTCTCTCTCAGACTCGAGG+AGG - chr2.1:18068853-18068872 None:intergenic 55.0%
GTGAAGGGACCAGTTAGGGA+GGG + chr2.1:18068815-18068834 MS.gene031256:CDS 55.0%
TCTCAGACTCGAGGAGGGTA+AGG - chr2.1:18068847-18068866 None:intergenic 55.0%
TGTGAAGGGACCAGTTAGGG+AGG + chr2.1:18068814-18068833 MS.gene031256:CDS 55.0%
! AACCTGAGTGACTTGTCCCC+TGG - chr2.1:18068750-18068769 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 18068692 18068889 18068692 ID=MS.gene031256
chr2.1 mRNA 18068692 18068889 18068692 ID=MS.gene031256.t1;Parent=MS.gene031256
chr2.1 exon 18068692 18068889 18068692 ID=MS.gene031256.t1.exon1;Parent=MS.gene031256.t1
chr2.1 CDS 18068692 18068889 18068692 ID=cds.MS.gene031256.t1;Parent=MS.gene031256.t1
Gene Sequence

>MS.gene031256

ATGGAATCACAGATTAAGCATGCTGTTGTTGTGAAAGTTATGGGTCGTACTGGATCCAGGGGACAAGTCACTCAGGTTAGAGTGAAGTTTTTGGATGATCAGAATCGTCACATCATGAGGAATGTGAAGGGACCAGTTAGGGAGGGAGACATCCTTACCCTCCTCGAGTCTGAGAGAGAAGCAAGAAGACTCCGATAG

Protein sequence

>MS.gene031256.t1

MESQIKHAVVVKVMGRTGSRGQVTQVRVKFLDDQNRHIMRNVKGPVREGDILTLLESEREARRLR