AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene007661

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene007661.t1 MTR_4g059400 99.398 166 1 0 1 166 1 166 1.89e-116 327
MS.gene007661.t1 MTR_1g064500 99.390 164 1 0 1 164 1 164 2.24e-114 321
MS.gene007661.t1 MTR_2g094300 83.537 164 27 0 1 164 1 164 1.50e-97 279
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene007661.t1 AT5G60670 90.361 166 16 0 1 166 1 166 9.53e-108 305
MS.gene007661.t1 AT2G37190 89.759 166 17 0 1 166 1 166 1.20e-107 304
MS.gene007661.t1 AT3G53430 89.157 166 18 0 1 166 1 166 2.70e-107 303

Find 64 sgRNAs with CRISPR-Local

Find 64 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTCTTCTTGCGGTCCCTTTC+TGG 0.085933 4.2:+44263703 None:intergenic
CGGAACGGTGAAGGAGATTT+TGG 0.108347 4.2:-44263587 MS.gene007661:CDS
GGACGTAAACTTCGACGATT+TGG 0.192090 4.2:+44263944 None:intergenic
CCAATCCTTGGCGGTTTCTT+TGG 0.201986 4.2:+44263828 None:intergenic
CCAAGGATTGGAAGGGTCTT+AGG 0.217246 4.2:-44263816 MS.gene007661:CDS
ATGTCTTCTCCGATCTTCTT+TGG 0.296410 4.2:+44263853 None:intergenic
ACTAAGAACATCAAGCATAA+TGG 0.336453 4.2:-44263682 MS.gene007661:CDS
TCTGTTGGGTGTACTGTTGA+TGG 0.361476 4.2:-44263553 MS.gene007661:CDS
CTAAGAACATCAAGCATAAT+GGG 0.363010 4.2:-44263681 MS.gene007661:CDS
GACGTAAACTTCGACGATTT+GGG 0.369524 4.2:+44263945 None:intergenic
CAAAATCTCCTTCACCGTTC+CGG 0.392918 4.2:+44263588 None:intergenic
ATCTCCTGCTGCAAATCCTT+TGG 0.401059 4.2:+44263523 None:intergenic
ATTTGGGATGGATCGAGCTT+TGG 0.421723 4.2:+44263961 None:intergenic
TTGGGGACTTGTGTTTCTGT+TGG 0.422484 4.2:-44263568 MS.gene007661:CDS
TGCGGCGGCGCTGGTTATCA+AGG 0.430495 4.2:-44263737 MS.gene007661:CDS
CCAAAGAAACCGCCAAGGAT+TGG 0.444226 4.2:-44263828 MS.gene007661:CDS
TGGGGACTTGTGTTTCTGTT+GGG 0.450633 4.2:-44263567 MS.gene007661:CDS
TGTTCCGTCTGCGGCGGCGC+TGG 0.455070 4.2:-44263746 MS.gene007661:CDS
GAGACCGAGAGGACCGATCT+TGG 0.459185 4.2:+44263879 None:intergenic
GGCTTTGAAGGAACCAGAAA+GGG 0.473237 4.2:-44263716 MS.gene007661:CDS
GATCTTGGGAGCGAGGGAAC+TGG 0.475143 4.2:+44263894 None:intergenic
GAAGTTTACGTCCGCGTCAC+CGG 0.477456 4.2:-44263934 MS.gene007661:CDS
GCTGGTTATCAAGGCTTTGA+AGG 0.478691 4.2:-44263728 MS.gene007661:CDS
AGACCGAGAGGACCGATCTT+GGG 0.482147 4.2:+44263880 None:intergenic
CGCGTCACCGGCGGTGAAGT+CGG 0.482477 4.2:-44263922 MS.gene007661:CDS
AGTTCCCTCGCTCCCAAGAT+CGG 0.485343 4.2:-44263892 MS.gene007661:CDS
TCGATGGCGAAGGAGCTTGC+CGG 0.485429 4.2:-44263607 MS.gene007661:CDS
CAAGGATTGGAAGGGTCTTA+GGG 0.485831 4.2:-44263815 MS.gene007661:CDS
AAGACCCTTCCAATCCTTGG+CGG 0.491678 4.2:+44263819 None:intergenic
GATTATGAAGCCGAGGTCGA+TGG 0.494531 4.2:-44263623 MS.gene007661:CDS
CTGTTGGGTGTACTGTTGAT+GGG 0.503488 4.2:-44263552 MS.gene007661:CDS
CCTAAGACCCTTCCAATCCT+TGG 0.507224 4.2:+44263816 None:intergenic
TGGGATGGATCGAGCTTTGG+CGG 0.507491 4.2:+44263964 None:intergenic
GGCGAAGGAGCTTGCCGGAA+CGG 0.508951 4.2:-44263602 MS.gene007661:CDS
CGCTGTTGTTCCGTCTGCGG+CGG 0.521792 4.2:-44263752 MS.gene007661:CDS
GCTCCCAAGATCGGTCCTCT+CGG 0.529023 4.2:-44263883 MS.gene007661:CDS
GGAGCTTGCCGGAACGGTGA+AGG 0.535229 4.2:-44263596 MS.gene007661:CDS
AGCTCCTTCGCCATCGACCT+CGG 0.537913 4.2:+44263613 None:intergenic
AGAAACCGCCAAGGATTGGA+AGG 0.540852 4.2:-44263824 MS.gene007661:CDS
CGTTCAGAATCGTCAGGCTA+AGG 0.541896 4.2:-44263776 MS.gene007661:CDS
GTTGAGATTCCTCAGGATTG+AGG 0.542687 4.2:-44263487 None:intergenic
GGCAGCTCCGACTTCACCGC+CGG 0.547290 4.2:+44263915 None:intergenic
GCTCACCGTTCAGAATCGTC+AGG 0.553915 4.2:-44263782 MS.gene007661:CDS
AGATCCAAAGGATTTGCAGC+AGG 0.556469 4.2:-44263527 MS.gene007661:CDS
AGGCTTTGAAGGAACCAGAA+AGG 0.556759 4.2:-44263717 MS.gene007661:CDS
TGATGTTCTTAGTCTTCTTG+CGG 0.558436 4.2:+44263692 None:intergenic
AGGACCGATCTTGGGAGCGA+GGG 0.558873 4.2:+44263888 None:intergenic
CGACTTCACCGCCGGTGACG+CGG 0.560163 4.2:+44263923 None:intergenic
TGGTGATGTTGAGATTCCTC+AGG 0.564181 4.2:-44263494 MS.gene007661:CDS
GAGGACCGATCTTGGGAGCG+AGG 0.581430 4.2:+44263887 None:intergenic
GGTCTCTCACCAAAGAAGAT+CGG 0.586947 4.2:-44263862 MS.gene007661:CDS
ATAACCAGCGCCGCCGCAGA+CGG 0.589868 4.2:+44263742 None:intergenic
TTGCAGCAGGAGATTAGTGA+TGG 0.592966 4.2:-44263514 MS.gene007661:CDS
GAAGCCGAGGTCGATGGCGA+AGG 0.599042 4.2:-44263617 MS.gene007661:CDS
GTTTACGTCCGCGTCACCGG+CGG 0.607165 4.2:-44263931 MS.gene007661:CDS
TAAACTTCGACGATTTGGGA+TGG 0.609008 4.2:+44263949 None:intergenic
TGTTGATGGGAAAGATCCAA+AGG 0.621231 4.2:-44263539 MS.gene007661:CDS
GGTCGCTGTTGTTCCGTCTG+CGG 0.635590 4.2:-44263755 MS.gene007661:CDS
TTGCGAAGATTATGAAGCCG+AGG 0.654828 4.2:-44263630 MS.gene007661:CDS
TTCTTTGGTGAGAGACCGAG+AGG 0.661014 4.2:+44263868 None:intergenic
CTTAGCCTGACGATTCTGAA+CGG 0.665775 4.2:+44263777 None:intergenic
GAAACCGCCAAGGATTGGAA+GGG 0.668035 4.2:-44263823 MS.gene007661:CDS
CATCGCCAAAGAAACCGCCA+AGG 0.687884 4.2:-44263833 MS.gene007661:CDS
CAACAACCACCTCAATCCTG+AGG 0.689306 4.2:+44263478 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
ACTAAGAACATCAAGCATAA+TGG - chr4.2:44263774-44263793 MS.gene007661:CDS 30.0%
CTAAGAACATCAAGCATAAT+GGG - chr4.2:44263775-44263794 MS.gene007661:CDS 30.0%
! TGATGTTCTTAGTCTTCTTG+CGG + chr4.2:44263767-44263786 None:intergenic 35.0%
ATGTCTTCTCCGATCTTCTT+TGG + chr4.2:44263606-44263625 None:intergenic 40.0%
GACGTAAACTTCGACGATTT+GGG + chr4.2:44263514-44263533 None:intergenic 40.0%
! TAAACTTCGACGATTTGGGA+TGG + chr4.2:44263510-44263529 None:intergenic 40.0%
! TGTTGATGGGAAAGATCCAA+AGG - chr4.2:44263917-44263936 MS.gene007661:CDS 40.0%
AGATCCAAAGGATTTGCAGC+AGG - chr4.2:44263929-44263948 MS.gene007661:CDS 45.0%
ATCTCCTGCTGCAAATCCTT+TGG + chr4.2:44263936-44263955 None:intergenic 45.0%
CAAAATCTCCTTCACCGTTC+CGG + chr4.2:44263871-44263890 None:intergenic 45.0%
CAAGGATTGGAAGGGTCTTA+GGG - chr4.2:44263641-44263660 MS.gene007661:CDS 45.0%
CTGTTGGGTGTACTGTTGAT+GGG - chr4.2:44263904-44263923 MS.gene007661:CDS 45.0%
CTTAGCCTGACGATTCTGAA+CGG + chr4.2:44263682-44263701 None:intergenic 45.0%
GGACGTAAACTTCGACGATT+TGG + chr4.2:44263515-44263534 None:intergenic 45.0%
GGTCTCTCACCAAAGAAGAT+CGG - chr4.2:44263594-44263613 MS.gene007661:CDS 45.0%
TCTGTTGGGTGTACTGTTGA+TGG - chr4.2:44263903-44263922 MS.gene007661:CDS 45.0%
TTGCAGCAGGAGATTAGTGA+TGG - chr4.2:44263942-44263961 MS.gene007661:CDS 45.0%
TTGCGAAGATTATGAAGCCG+AGG - chr4.2:44263826-44263845 MS.gene007661:CDS 45.0%
! ATTTGGGATGGATCGAGCTT+TGG + chr4.2:44263498-44263517 None:intergenic 45.0%
! GAACGGTGAAGGAGATTTTG+GGG - chr4.2:44263871-44263890 MS.gene007661:CDS 45.0%
! GCTGGTTATCAAGGCTTTGA+AGG - chr4.2:44263728-44263747 MS.gene007661:CDS 45.0%
! GGAACGGTGAAGGAGATTTT+GGG - chr4.2:44263870-44263889 MS.gene007661:CDS 45.0%
! TGGGGACTTGTGTTTCTGTT+GGG - chr4.2:44263889-44263908 MS.gene007661:CDS 45.0%
! TGGTGATGTTGAGATTCCTC+AGG - chr4.2:44263962-44263981 MS.gene007661:CDS 45.0%
! TTGGGGACTTGTGTTTCTGT+TGG - chr4.2:44263888-44263907 MS.gene007661:CDS 45.0%
!! AGGCTTTGAAGGAACCAGAA+AGG - chr4.2:44263739-44263758 MS.gene007661:CDS 45.0%
!! GGCTTTGAAGGAACCAGAAA+GGG - chr4.2:44263740-44263759 MS.gene007661:CDS 45.0%
AAGACCCTTCCAATCCTTGG+CGG + chr4.2:44263640-44263659 None:intergenic 50.0%
AGAAACCGCCAAGGATTGGA+AGG - chr4.2:44263632-44263651 MS.gene007661:CDS 50.0%
CCAAAGAAACCGCCAAGGAT+TGG - chr4.2:44263628-44263647 MS.gene007661:CDS 50.0%
CCAAGGATTGGAAGGGTCTT+AGG - chr4.2:44263640-44263659 MS.gene007661:CDS 50.0%
CCAATCCTTGGCGGTTTCTT+TGG + chr4.2:44263631-44263650 None:intergenic 50.0%
CCTAAGACCCTTCCAATCCT+TGG + chr4.2:44263643-44263662 None:intergenic 50.0%
CGTTCAGAATCGTCAGGCTA+AGG - chr4.2:44263680-44263699 MS.gene007661:CDS 50.0%
GAAACCGCCAAGGATTGGAA+GGG - chr4.2:44263633-44263652 MS.gene007661:CDS 50.0%
GATTATGAAGCCGAGGTCGA+TGG - chr4.2:44263833-44263852 MS.gene007661:CDS 50.0%
TTCTTTGGTGAGAGACCGAG+AGG + chr4.2:44263591-44263610 None:intergenic 50.0%
! CGGAACGGTGAAGGAGATTT+TGG - chr4.2:44263869-44263888 MS.gene007661:CDS 50.0%
AGACCGAGAGGACCGATCTT+GGG + chr4.2:44263579-44263598 None:intergenic 55.0%
AGTTCCCTCGCTCCCAAGAT+CGG - chr4.2:44263564-44263583 MS.gene007661:CDS 55.0%
CATCGCCAAAGAAACCGCCA+AGG - chr4.2:44263623-44263642 MS.gene007661:CDS 55.0%
GAAGTTTACGTCCGCGTCAC+CGG - chr4.2:44263522-44263541 MS.gene007661:CDS 55.0%
GCTCACCGTTCAGAATCGTC+AGG - chr4.2:44263674-44263693 MS.gene007661:CDS 55.0%
GTCTTCTTGCGGTCCCTTTC+TGG + chr4.2:44263756-44263775 None:intergenic 55.0%
! TGGGATGGATCGAGCTTTGG+CGG + chr4.2:44263495-44263514 None:intergenic 55.0%
AGCTCCTTCGCCATCGACCT+CGG + chr4.2:44263846-44263865 None:intergenic 60.0%
AGGACCGATCTTGGGAGCGA+GGG + chr4.2:44263571-44263590 None:intergenic 60.0%
GAGACCGAGAGGACCGATCT+TGG + chr4.2:44263580-44263599 None:intergenic 60.0%
GATCTTGGGAGCGAGGGAAC+TGG + chr4.2:44263565-44263584 None:intergenic 60.0%
GCTCCCAAGATCGGTCCTCT+CGG - chr4.2:44263573-44263592 MS.gene007661:CDS 60.0%
GGTCGCTGTTGTTCCGTCTG+CGG - chr4.2:44263701-44263720 MS.gene007661:CDS 60.0%
TCGATGGCGAAGGAGCTTGC+CGG - chr4.2:44263849-44263868 MS.gene007661:CDS 60.0%
ATAACCAGCGCCGCCGCAGA+CGG + chr4.2:44263717-44263736 None:intergenic 65.0%
CGCTGTTGTTCCGTCTGCGG+CGG - chr4.2:44263704-44263723 MS.gene007661:CDS 65.0%
GAAGCCGAGGTCGATGGCGA+AGG - chr4.2:44263839-44263858 MS.gene007661:CDS 65.0%
GAGGACCGATCTTGGGAGCG+AGG + chr4.2:44263572-44263591 None:intergenic 65.0%
GGAGCTTGCCGGAACGGTGA+AGG - chr4.2:44263860-44263879 MS.gene007661:CDS 65.0%
GGCGAAGGAGCTTGCCGGAA+CGG - chr4.2:44263854-44263873 MS.gene007661:CDS 65.0%
GTTTACGTCCGCGTCACCGG+CGG - chr4.2:44263525-44263544 MS.gene007661:CDS 65.0%
!! TGCGGCGGCGCTGGTTATCA+AGG - chr4.2:44263719-44263738 MS.gene007661:CDS 65.0%
CGACTTCACCGCCGGTGACG+CGG + chr4.2:44263536-44263555 None:intergenic 70.0%
CGCGTCACCGGCGGTGAAGT+CGG - chr4.2:44263534-44263553 MS.gene007661:CDS 70.0%
GGCAGCTCCGACTTCACCGC+CGG + chr4.2:44263544-44263563 None:intergenic 70.0%
TGTTCCGTCTGCGGCGGCGC+TGG - chr4.2:44263710-44263729 MS.gene007661:CDS 75.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 44263489 44263989 44263489 ID=MS.gene007661
chr4.2 mRNA 44263489 44263989 44263489 ID=MS.gene007661.t1;Parent=MS.gene007661
chr4.2 exon 44263489 44263989 44263489 ID=MS.gene007661.t1.exon1;Parent=MS.gene007661.t1
chr4.2 CDS 44263489 44263989 44263489 ID=cds.MS.gene007661.t1;Parent=MS.gene007661.t1
Gene Sequence

>MS.gene007661

ATGCCGCCAAAGCTCGATCCATCCCAAATCGTCGAAGTTTACGTCCGCGTCACCGGCGGTGAAGTCGGAGCTGCCAGTTCCCTCGCTCCCAAGATCGGTCCTCTCGGTCTCTCACCAAAGAAGATCGGAGAAGACATCGCCAAAGAAACCGCCAAGGATTGGAAGGGTCTTAGGGTGACAGTCAAGCTCACCGTTCAGAATCGTCAGGCTAAGGTCGCTGTTGTTCCGTCTGCGGCGGCGCTGGTTATCAAGGCTTTGAAGGAACCAGAAAGGGACCGCAAGAAGACTAAGAACATCAAGCATAATGGGAATATCTCTCTTGATGATGTTATTGAGATTGCGAAGATTATGAAGCCGAGGTCGATGGCGAAGGAGCTTGCCGGAACGGTGAAGGAGATTTTGGGGACTTGTGTTTCTGTTGGGTGTACTGTTGATGGGAAAGATCCAAAGGATTTGCAGCAGGAGATTAGTGATGGTGATGTTGAGATTCCTCAGGATTGA
Protein sequence

>MS.gene007661.t1

MPPKLDPSQIVEVYVRVTGGEVGAASSLAPKIGPLGLSPKKIGEDIAKETAKDWKGLRVTVKLTVQNRQAKVAVVPSAAALVIKALKEPERDRKKTKNIKHNGNISLDDVIEIAKIMKPRSMAKELAGTVKEILGTCVSVGCTVDGKDPKDLQQEISDGDVEIPQD