AlfalfaGEDB | MS.gene24598

M. sativa cultivar XinJiangDaYe / MS.gene24598

KEGG: K02870
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0019538 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576
PFAM: PF00298.22 PF03946.17

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene24598.t1	XP_013468200.1	100	166	0	0	1	166	1	166	2.10E-84	321.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene24598.t1	O50003	93.4	166	11	0	1	166	1	166	9.3e-81	300.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene24598.t1	I3SXZ6	100.0	166	0	0	1	166	1	166	1.5e-84	321.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene045180	MS.gene24598	PPI
MS.gene007662	MS.gene24598	PPI
MS.gene45417	MS.gene24598	PPI
MS.gene056257	MS.gene24598	PPI
MS.gene08871	MS.gene24598	PPI
MS.gene039110	MS.gene24598	PPI
MS.gene054186	MS.gene24598	PPI
MS.gene060216	MS.gene24598	PPI
MS.gene24598	MS.gene63721	PPI
MS.gene059422	MS.gene24598	PPI
MS.gene050134	MS.gene24598	PPI
MS.gene08636	MS.gene24598	PPI
MS.gene037324	MS.gene24598	PPI
MS.gene75537	MS.gene24598	PPI
MS.gene24598	MS.gene067072	PPI
MS.gene063366	MS.gene24598	PPI
MS.gene41916	MS.gene24598	PPI
MS.gene066163	MS.gene24598	PPI
MS.gene040581	MS.gene24598	PPI
MS.gene007661	MS.gene24598	PPI
MS.gene99744	MS.gene24598	PPI
MS.gene029242	MS.gene24598	PPI
MS.gene029840	MS.gene24598	PPI
MS.gene24598	MS.gene001986	PPI
MS.gene08870	MS.gene24598	PPI

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene24598.t1	MTR_1g064500	100.000	166	0	1	166	1	166	1.17e-116	327
MS.gene24598.t1	MTR_4g059400	99.390	164	1	1	164	1	164	1.21e-114	322
MS.gene24598.t1	MTR_2g094300	83.537	164	27	1	164	1	164	1.22e-97	279

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene24598.t1	AT5G60670	90.964	166	15	1	166	1	166	1.27e-107	304
MS.gene24598.t1	AT2G37190	90.854	164	15	1	164	1	164	5.76e-107	303
MS.gene24598.t1	AT3G53430	90.244	164	16	1	164	1	164	1.10e-106	302

Find 56 sgRNAs with CRISPR-Local

Find 58 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CAACCTTCGCTTGACGATTC+TGG	0.204840	1.3:-46483038	None:intergenic
ATGTCTTCTCCGATCTTCTT+TGG	0.275399	1.3:-46482958	None:intergenic
ACGAAGAATATTAAGCATAA+TGG	0.343409	1.3:+46483129	MS.gene24598:CDS
ATCTGTGATGGGTCGAGCTT+TGG	0.352336	1.3:-46482850	None:intergenic
CTTGGAACTTGTGTTTCTGT+TGG	0.355041	1.3:+46483243	MS.gene24598:CDS
CGAAGAATATTAAGCATAAT+GGG	0.358983	1.3:+46483130	MS.gene24598:CDS
TCTGTTGGGTGTACTGTTGA+TGG	0.361476	1.3:+46483258	MS.gene24598:CDS
CGCGGCGGCGCTTGTGATCA+AGG	0.362603	1.3:+46483074	MS.gene24598:CDS
AATCTCTTGTTGCAAATCCT+TGG	0.364185	1.3:-46483289	None:intergenic
ATCTCTTGTTGCAAATCCTT+GGG	0.391437	1.3:-46483288	None:intergenic
TTGGAACTTGTGTTTCTGTT+GGG	0.400252	1.3:+46483244	MS.gene24598:CDS
TGAAGGAACCAGAGAGAGAT+AGG	0.404063	1.3:+46483100	MS.gene24598:CDS
GTCTTCTTCCTATCTCTCTC+TGG	0.414689	1.3:-46483108	None:intergenic
GAGACCGAGGGGACCGATCT+TGG	0.416952	1.3:-46482932	None:intergenic
CCAAAGAAACCGCAAAAGAC+TGG	0.428144	1.3:+46482983	MS.gene24598:CDS
AGACCGAGGGGACCGATCTT+GGG	0.428565	1.3:-46482931	None:intergenic
GATCTTGGGAGCGAGGGAAC+TGG	0.450092	1.3:-46482917	None:intergenic
GGTACTGTGAAGGAGATTCT+TGG	0.451833	1.3:+46483225	MS.gene24598:CDS
TGTGATGGGTCGAGCTTTGG+AGG	0.459117	1.3:-46482847	None:intergenic
TCCATGGCGAAGGAGCTTGC+GGG	0.471935	1.3:+46483204	MS.gene24598:CDS
AGCTCCTTCGCCATGGATCT+CGG	0.474540	1.3:-46483198	None:intergenic
GATTATGAAGCCGAGATCCA+TGG	0.485064	1.3:+46483188	MS.gene24598:CDS
GAAGTCTACGTCCGCGTCAC+CGG	0.489324	1.3:+46482877	MS.gene24598:CDS
AGTTCCCTCGCTCCCAAGAT+CGG	0.497799	1.3:+46482919	MS.gene24598:CDS
CTGTTGGGTGTACTGTTGAT+GGG	0.503488	1.3:+46483259	MS.gene24598:CDS
GGGACCGATCTTGGGAGCGA+GGG	0.509084	1.3:-46482923	None:intergenic
CGCGTCACCGGTGGAGAAGT+CGG	0.509186	1.3:+46482889	MS.gene24598:CDS
GGGGACCGATCTTGGGAGCG+AGG	0.533033	1.3:-46482924	None:intergenic
ATCCATGGCGAAGGAGCTTG+CGG	0.534410	1.3:+46483203	MS.gene24598:CDS
GCTCCCAAGATCGGTCCCCT+CGG	0.536064	1.3:+46482928	MS.gene24598:CDS
GCTTGTGATCAAGGCGTTGA+AGG	0.539531	1.3:+46483083	MS.gene24598:CDS
ATCACAAGCGCCGCCGCGGA+GGG	0.546925	1.3:-46483069	None:intergenic
GGCGGCGCCGACTTCTCCAC+CGG	0.558047	1.3:-46482896	None:intergenic
GTCTACGTCCGCGTCACCGG+TGG	0.568502	1.3:+46482880	MS.gene24598:CDS
TTGCAACAAGAGATTGATGA+TGG	0.571171	1.3:+46483297	MS.gene24598:CDS
ACCCGCAAGCTCCTTCGCCA+TGG	0.574079	1.3:-46483205	None:intergenic
CTTGGGAGCGAGGGAACTGG+CGG	0.575994	1.3:-46482914	None:intergenic
GGTCTCTCTCCAAAGAAGAT+CGG	0.584278	1.3:+46482949	MS.gene24598:CDS
GATCACAAGCGCCGCCGCGG+AGG	0.585182	1.3:-46483070	None:intergenic
TAGACTTCGACGATCTGTGA+TGG	0.587094	1.3:-46482862	None:intergenic
CGACTTCTCCACCGGTGACG+CGG	0.588865	1.3:-46482888	None:intergenic
AGAAACCGCAAAAGACTGGA+AGG	0.591239	1.3:+46482987	MS.gene24598:CDS
CTTCTTTGGAGAGAGACCGA+GGG	0.594178	1.3:-46482944	None:intergenic
GGAGCTTGCGGGTACTGTGA+AGG	0.597173	1.3:+46483215	MS.gene24598:CDS
TGCGGTTGTTCCCTCCGCGG+CGG	0.605465	1.3:+46483059	MS.gene24598:CDS
TCTTCTTTGGAGAGAGACCG+AGG	0.616068	1.3:-46482945	None:intergenic
AGACTTCGACGATCTGTGAT+GGG	0.618253	1.3:-46482861	None:intergenic
GAATCGTCAAGCGAAGGTTG+CGG	0.619107	1.3:+46483041	MS.gene24598:CDS
CTTGATCACAAGCGCCGCCG+CGG	0.628883	1.3:-46483073	None:intergenic
TGTTGATGGGAAAGATCCCA+AGG	0.632585	1.3:+46483272	MS.gene24598:CDS
AGTCCAGAATCGTCAAGCGA+AGG	0.641493	1.3:+46483035	MS.gene24598:CDS
GAAGCCGAGATCCATGGCGA+AGG	0.654215	1.3:+46483194	MS.gene24598:CDS
CTGTGAGCTTGACTGTGACA+CGG	0.663078	1.3:-46483014	None:intergenic
GAAACCGCAAAAGACTGGAA+GGG	0.670069	1.3:+46482988	MS.gene24598:CDS
GGTTGCGGTTGTTCCCTCCG+CGG	0.676784	1.3:+46483056	MS.gene24598:CDS
TTCTTTGGAGAGAGACCGAG+GGG	0.684696	1.3:-46482943	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	ACGAAGAATATTAAGCATAA+TGG	+	chr1.3:46483129-46483148	MS.gene24598:CDS	25.0%
!	CGAAGAATATTAAGCATAAT+GGG	+	chr1.3:46483130-46483149	MS.gene24598:CDS	25.0%
	AATCTCTTGTTGCAAATCCT+TGG	-	chr1.3:46483292-46483311	None:intergenic	35.0%
	ATCTCTTGTTGCAAATCCTT+GGG	-	chr1.3:46483291-46483310	None:intergenic	35.0%
	TTGCAACAAGAGATTGATGA+TGG	+	chr1.3:46483297-46483316	MS.gene24598:CDS	35.0%
!	TTGGAACTTGTGTTTCTGTT+GGG	+	chr1.3:46483244-46483263	MS.gene24598:CDS	35.0%
	ATGTCTTCTCCGATCTTCTT+TGG	-	chr1.3:46482961-46482980	None:intergenic	40.0%
!	CTTGGAACTTGTGTTTCTGT+TGG	+	chr1.3:46483243-46483262	MS.gene24598:CDS	40.0%
	AGAAACCGCAAAAGACTGGA+AGG	+	chr1.3:46482987-46483006	MS.gene24598:CDS	45.0%
	AGACTTCGACGATCTGTGAT+GGG	-	chr1.3:46482864-46482883	None:intergenic	45.0%
	CCAAAGAAACCGCAAAAGAC+TGG	+	chr1.3:46482983-46483002	MS.gene24598:CDS	45.0%
	CTGTTGGGTGTACTGTTGAT+GGG	+	chr1.3:46483259-46483278	MS.gene24598:CDS	45.0%
	GAAACCGCAAAAGACTGGAA+GGG	+	chr1.3:46482988-46483007	MS.gene24598:CDS	45.0%
	GATTATGAAGCCGAGATCCA+TGG	+	chr1.3:46483188-46483207	MS.gene24598:CDS	45.0%
	GGTACTGTGAAGGAGATTCT+TGG	+	chr1.3:46483225-46483244	MS.gene24598:CDS	45.0%
	GGTCTCTCTCCAAAGAAGAT+CGG	+	chr1.3:46482949-46482968	MS.gene24598:CDS	45.0%
	GTCTTCTTCCTATCTCTCTC+TGG	-	chr1.3:46483111-46483130	None:intergenic	45.0%
	TAGACTTCGACGATCTGTGA+TGG	-	chr1.3:46482865-46482884	None:intergenic	45.0%
	TCTGTTGGGTGTACTGTTGA+TGG	+	chr1.3:46483258-46483277	MS.gene24598:CDS	45.0%
	TGAAGGAACCAGAGAGAGAT+AGG	+	chr1.3:46483100-46483119	MS.gene24598:CDS	45.0%
!	CCAGTCTTTTGCGGTTTCTT+TGG	-	chr1.3:46482986-46483005	None:intergenic	45.0%
!	TGTTGATGGGAAAGATCCCA+AGG	+	chr1.3:46483272-46483291	MS.gene24598:CDS	45.0%
	AGTCCAGAATCGTCAAGCGA+AGG	+	chr1.3:46483035-46483054	MS.gene24598:CDS	50.0%
	CAACCTTCGCTTGACGATTC+TGG	-	chr1.3:46483041-46483060	None:intergenic	50.0%
	CTGTGAGCTTGACTGTGACA+CGG	-	chr1.3:46483017-46483036	None:intergenic	50.0%
	GAATCGTCAAGCGAAGGTTG+CGG	+	chr1.3:46483041-46483060	MS.gene24598:CDS	50.0%
!	CTTCTTTGGAGAGAGACCGA+GGG	-	chr1.3:46482947-46482966	None:intergenic	50.0%
!	GAGACCCTTCCAGTCTTTTG+CGG	-	chr1.3:46482995-46483014	None:intergenic	50.0%
!	TCTTCTTTGGAGAGAGACCG+AGG	-	chr1.3:46482948-46482967	None:intergenic	50.0%
!	TTCTTTGGAGAGAGACCGAG+GGG	-	chr1.3:46482946-46482965	None:intergenic	50.0%
!!	ATCTGTGATGGGTCGAGCTT+TGG	-	chr1.3:46482853-46482872	None:intergenic	50.0%
!!	GCTTGTGATCAAGGCGTTGA+AGG	+	chr1.3:46483083-46483102	MS.gene24598:CDS	50.0%
	AGCTCCTTCGCCATGGATCT+CGG	-	chr1.3:46483201-46483220	None:intergenic	55.0%
	AGTTCCCTCGCTCCCAAGAT+CGG	+	chr1.3:46482919-46482938	MS.gene24598:CDS	55.0%
	ATCCATGGCGAAGGAGCTTG+CGG	+	chr1.3:46483203-46483222	MS.gene24598:CDS	55.0%
!!	TGTGATGGGTCGAGCTTTGG+AGG	-	chr1.3:46482850-46482869	None:intergenic	55.0%
	AGACCGAGGGGACCGATCTT+GGG	-	chr1.3:46482934-46482953	None:intergenic	60.0%
	GAAGCCGAGATCCATGGCGA+AGG	+	chr1.3:46483194-46483213	MS.gene24598:CDS	60.0%
	GAAGTCTACGTCCGCGTCAC+CGG	+	chr1.3:46482877-46482896	MS.gene24598:CDS	60.0%
	GATCTTGGGAGCGAGGGAAC+TGG	-	chr1.3:46482920-46482939	None:intergenic	60.0%
	TCCATGGCGAAGGAGCTTGC+GGG	+	chr1.3:46483204-46483223	MS.gene24598:CDS	60.0%
!	GGAGCTTGCGGGTACTGTGA+AGG	+	chr1.3:46483215-46483234	MS.gene24598:CDS	60.0%
	ACCCGCAAGCTCCTTCGCCA+TGG	-	chr1.3:46483208-46483227	None:intergenic	65.0%
	CGACTTCTCCACCGGTGACG+CGG	-	chr1.3:46482891-46482910	None:intergenic	65.0%
	CGCGTCACCGGTGGAGAAGT+CGG	+	chr1.3:46482889-46482908	MS.gene24598:CDS	65.0%
	CTTGGGAGCGAGGGAACTGG+CGG	-	chr1.3:46482917-46482936	None:intergenic	65.0%
	GAGACCGAGGGGACCGATCT+TGG	-	chr1.3:46482935-46482954	None:intergenic	65.0%
	GCTCCCAAGATCGGTCCCCT+CGG	+	chr1.3:46482928-46482947	MS.gene24598:CDS	65.0%
	GGGACCGATCTTGGGAGCGA+GGG	-	chr1.3:46482926-46482945	None:intergenic	65.0%
	GGTTGCGGTTGTTCCCTCCG+CGG	+	chr1.3:46483056-46483075	MS.gene24598:CDS	65.0%
!	CTTGATCACAAGCGCCGCCG+CGG	-	chr1.3:46483076-46483095	None:intergenic	65.0%
	CGCGGCGGCGCTTGTGATCA+AGG	+	chr1.3:46483074-46483093	MS.gene24598:CDS	70.0%
	GGGGACCGATCTTGGGAGCG+AGG	-	chr1.3:46482927-46482946	None:intergenic	70.0%
	GTCTACGTCCGCGTCACCGG+TGG	+	chr1.3:46482880-46482899	MS.gene24598:CDS	70.0%
	TGCGGTTGTTCCCTCCGCGG+CGG	+	chr1.3:46483059-46483078	MS.gene24598:CDS	70.0%
!	ATCACAAGCGCCGCCGCGGA+GGG	-	chr1.3:46483072-46483091	None:intergenic	70.0%
!	GATCACAAGCGCCGCCGCGG+AGG	-	chr1.3:46483073-46483092	None:intergenic	75.0%
!!	GGCGGCGCCGACTTCTCCAC+CGG	-	chr1.3:46482899-46482918	None:intergenic	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.3	gene	46482844	46483344	46482844	ID=MS.gene24598
chr1.3	mRNA	46482844	46483344	46482844	ID=MS.gene24598.t1;Parent=MS.gene24598
chr1.3	exon	46482844	46483344	46482844	ID=MS.gene24598.t1.exon1;Parent=MS.gene24598.t1
chr1.3	CDS	46482844	46483344	46482844	ID=cds.MS.gene24598.t1;Parent=MS.gene24598.t1

Gene Sequence

>MS.gene24598

ATGCCTCCAAAGCTCGACCCATCACAGATCGTCGAAGTCTACGTCCGCGTCACCGGTGGAGAAGTCGGCGCCGCCAGTTCCCTCGCTCCCAAGATCGGTCCCCTCGGTCTCTCTCCAAAGAAGATCGGAGAAGACATCGCCAAAGAAACCGCAAAAGACTGGAAGGGTCTCCGTGTCACAGTCAAGCTCACAGTCCAGAATCGTCAAGCGAAGGTTGCGGTTGTTCCCTCCGCGGCGGCGCTTGTGATCAAGGCGTTGAAGGAACCAGAGAGAGATAGGAAGAAGACGAAGAATATTAAGCATAATGGGAATATCTCACTTGATGATGTTATTGAGATTGCGAAGATTATGAAGCCGAGATCCATGGCGAAGGAGCTTGCGGGTACTGTGAAGGAGATTCTTGGAACTTGTGTTTCTGTTGGGTGTACTGTTGATGGGAAAGATCCCAAGGATTTGCAACAAGAGATTGATGATGGTGATGTTGAGATTCCTTTAGAATAA

Protein sequence

>MS.gene24598.t1

MPPKLDPSQIVEVYVRVTGGEVGAASSLAPKIGPLGLSPKKIGEDIAKETAKDWKGLRVTVKLTVQNRQAKVAVVPSAAALVIKALKEPERDRKKTKNIKHNGNISLDDVIEIAKIMKPRSMAKELAGTVKEILGTCVSVGCTVDGKDPKDLQQEIDDGDVEIPLE