AlfalfaGEDB | MS.gene007662

M. sativa cultivar XinJiangDaYe / MS.gene007662

KEGG: K02870
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0019538 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576
PFAM: PF00298.22 PF03946.17

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene007662.t1	XP_003606385.1	99.4	166	1	0	1	166	1	166	8.00E-84	319.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene007662.t1	O50003	93.4	166	11	0	1	166	1	166	1.2e-80	300.4

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene007662.t1	B7FH91	99.4	166	1	0	1	166	1	166	5.8e-84	319.7

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene99744	MS.gene007662	PPI
MS.gene029242	MS.gene007662	PPI
MS.gene007662	MS.gene24598	PPI
MS.gene007662	MS.gene067072	PPI
MS.gene007662	MS.gene050134	PPI
MS.gene007662	MS.gene41916	PPI
MS.gene08870	MS.gene007662	PPI
MS.gene045180	MS.gene007662	PPI
MS.gene007662	MS.gene001986	PPI
MS.gene08871	MS.gene007662	PPI
MS.gene007662	MS.gene060216	PPI
MS.gene007662	MS.gene029840	PPI
MS.gene007662	MS.gene054186	PPI
MS.gene007662	MS.gene056257	PPI
MS.gene007662	MS.gene039110	PPI
MS.gene08636	MS.gene007662	PPI
MS.gene45417	MS.gene007662	PPI
MS.gene007662	MS.gene63721	PPI
MS.gene063366	MS.gene007662	PPI
MS.gene066163	MS.gene007662	PPI
MS.gene059422	MS.gene007662	PPI
MS.gene040581	MS.gene007662	PPI
MS.gene007661	MS.gene007662	PPI
MS.gene037324	MS.gene007662	PPI
MS.gene007662	MS.gene75537	PPI

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene007662.t1	MTR_4g059400	99.398	166	1	1	166	1	166	1.89e-116	327
MS.gene007662.t1	MTR_1g064500	99.390	164	1	1	164	1	164	2.24e-114	321
MS.gene007662.t1	MTR_2g094300	83.537	164	27	1	164	1	164	1.50e-97	279

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene007662.t1	AT5G60670	90.361	166	16	1	166	1	166	9.53e-108	305
MS.gene007662.t1	AT2G37190	89.759	166	17	1	166	1	166	1.20e-107	304
MS.gene007662.t1	AT3G53430	89.157	166	18	1	166	1	166	2.70e-107	303

Find 64 sgRNAs with CRISPR-Local

Find 64 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GTCTTCTTGCGGTCCCTTTC+TGG	0.085933	4.3:+42324663	None:intergenic
CGGAACGGTGAAGGAGATTT+TGG	0.108347	4.3:-42324547	MS.gene007662:CDS
GGACGTAAACTTCGACGATT+TGG	0.192090	4.3:+42324904	None:intergenic
CCAATCCTTGGCGGTTTCTT+TGG	0.201986	4.3:+42324788	None:intergenic
CCAAGGATTGGAAGGGTCTT+AGG	0.217246	4.3:-42324776	MS.gene007662:CDS
ATGTCTTCTCCGATCTTCTT+TGG	0.296410	4.3:+42324813	None:intergenic
ACTAAGAACATCAAGCATAA+TGG	0.336453	4.3:-42324642	MS.gene007662:CDS
TCTGTTGGGTGTACTGTTGA+TGG	0.361476	4.3:-42324513	MS.gene007662:CDS
CTAAGAACATCAAGCATAAT+GGG	0.363010	4.3:-42324641	MS.gene007662:CDS
GACGTAAACTTCGACGATTT+GGG	0.369524	4.3:+42324905	None:intergenic
CAAAATCTCCTTCACCGTTC+CGG	0.392918	4.3:+42324548	None:intergenic
ATCTCCTGCTGCAAATCCTT+TGG	0.401059	4.3:+42324483	None:intergenic
ATTTGGGATGGATCGAGCTT+TGG	0.421723	4.3:+42324921	None:intergenic
TTGGGGACTTGTGTTTCTGT+TGG	0.422484	4.3:-42324528	MS.gene007662:CDS
TGCGGCGGCGCTGGTTATCA+AGG	0.430495	4.3:-42324697	MS.gene007662:CDS
CCAAAGAAACCGCCAAGGAT+TGG	0.444226	4.3:-42324788	MS.gene007662:CDS
TGGGGACTTGTGTTTCTGTT+GGG	0.450633	4.3:-42324527	MS.gene007662:CDS
TGTTCCGTCTGCGGCGGCGC+TGG	0.455070	4.3:-42324706	MS.gene007662:CDS
GAGACCGAGAGGACCGATCT+TGG	0.459185	4.3:+42324839	None:intergenic
GGCTTTGAAGGAACCAGAAA+GGG	0.473237	4.3:-42324676	MS.gene007662:CDS
GATCTTGGGAGCGAGGGAAC+TGG	0.475143	4.3:+42324854	None:intergenic
GAAGTTTACGTCCGCGTCAC+CGG	0.477456	4.3:-42324894	MS.gene007662:CDS
GCTGGTTATCAAGGCTTTGA+AGG	0.478691	4.3:-42324688	MS.gene007662:CDS
AGACCGAGAGGACCGATCTT+GGG	0.482147	4.3:+42324840	None:intergenic
CGCGTCACCGGCGGTGAAGT+CGG	0.482477	4.3:-42324882	MS.gene007662:CDS
AGTTCCCTCGCTCCCAAGAT+CGG	0.485343	4.3:-42324852	MS.gene007662:CDS
TCGATGGCGAAGGAGCTTGC+CGG	0.485429	4.3:-42324567	MS.gene007662:CDS
CAAGGATTGGAAGGGTCTTA+GGG	0.485831	4.3:-42324775	MS.gene007662:CDS
AAGACCCTTCCAATCCTTGG+CGG	0.491678	4.3:+42324779	None:intergenic
GATTATGAAGCCGAGGTCGA+TGG	0.494531	4.3:-42324583	MS.gene007662:CDS
CTGTTGGGTGTACTGTTGAT+GGG	0.503488	4.3:-42324512	MS.gene007662:CDS
CCTAAGACCCTTCCAATCCT+TGG	0.507224	4.3:+42324776	None:intergenic
TGGGATGGATCGAGCTTTGG+CGG	0.507491	4.3:+42324924	None:intergenic
GGCGAAGGAGCTTGCCGGAA+CGG	0.508951	4.3:-42324562	MS.gene007662:CDS
CGCTGTTGTTCCGTCTGCGG+CGG	0.521792	4.3:-42324712	MS.gene007662:CDS
GCTCCCAAGATCGGTCCTCT+CGG	0.529023	4.3:-42324843	MS.gene007662:CDS
GGAGCTTGCCGGAACGGTGA+AGG	0.535229	4.3:-42324556	MS.gene007662:CDS
AGCTCCTTCGCCATCGACCT+CGG	0.537913	4.3:+42324573	None:intergenic
AGAAACCGCCAAGGATTGGA+AGG	0.540852	4.3:-42324784	MS.gene007662:CDS
CGTTCAGAATCGTCAGGCTA+AGG	0.541896	4.3:-42324736	MS.gene007662:CDS
GTTGAGATTCCTCAGGATTG+AGG	0.542687	4.3:-42324447	None:intergenic
GGCAGCTCCGACTTCACCGC+CGG	0.547290	4.3:+42324875	None:intergenic
GCTCACCGTTCAGAATCGTC+AGG	0.553915	4.3:-42324742	MS.gene007662:CDS
AGATCCAAAGGATTTGCAGC+AGG	0.556469	4.3:-42324487	MS.gene007662:CDS
AGGCTTTGAAGGAACCAGAA+AGG	0.556759	4.3:-42324677	MS.gene007662:CDS
TGATGTTCTTAGTCTTCTTG+CGG	0.558436	4.3:+42324652	None:intergenic
AGGACCGATCTTGGGAGCGA+GGG	0.558873	4.3:+42324848	None:intergenic
CGACTTCACCGCCGGTGACG+CGG	0.560163	4.3:+42324883	None:intergenic
TGGTGATGTTGAGATTCCTC+AGG	0.564181	4.3:-42324454	MS.gene007662:CDS
GAGGACCGATCTTGGGAGCG+AGG	0.581430	4.3:+42324847	None:intergenic
GGTCTCTCACCAAAGAAGAT+CGG	0.586947	4.3:-42324822	MS.gene007662:CDS
ATAACCAGCGCCGCCGCAGA+CGG	0.589868	4.3:+42324702	None:intergenic
TTGCAGCAGGAGATTAGTGA+TGG	0.592966	4.3:-42324474	MS.gene007662:CDS
GAAGCCGAGGTCGATGGCGA+AGG	0.599042	4.3:-42324577	MS.gene007662:CDS
GTTTACGTCCGCGTCACCGG+CGG	0.607165	4.3:-42324891	MS.gene007662:CDS
TAAACTTCGACGATTTGGGA+TGG	0.609008	4.3:+42324909	None:intergenic
TGTTGATGGGAAAGATCCAA+AGG	0.621231	4.3:-42324499	MS.gene007662:CDS
GGTCGCTGTTGTTCCGTCTG+CGG	0.635590	4.3:-42324715	MS.gene007662:CDS
TTGCGAAGATTATGAAGCCG+AGG	0.654828	4.3:-42324590	MS.gene007662:CDS
TTCTTTGGTGAGAGACCGAG+AGG	0.661014	4.3:+42324828	None:intergenic
CTTAGCCTGACGATTCTGAA+CGG	0.665775	4.3:+42324737	None:intergenic
GAAACCGCCAAGGATTGGAA+GGG	0.668035	4.3:-42324783	MS.gene007662:CDS
CATCGCCAAAGAAACCGCCA+AGG	0.687884	4.3:-42324793	MS.gene007662:CDS
CAACAACCACCTCAATCCTG+AGG	0.689306	4.3:+42324438	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	ACTAAGAACATCAAGCATAA+TGG	-	chr4.3:42324734-42324753	MS.gene007662:CDS	30.0%
	CTAAGAACATCAAGCATAAT+GGG	-	chr4.3:42324735-42324754	MS.gene007662:CDS	30.0%
!	TGATGTTCTTAGTCTTCTTG+CGG	+	chr4.3:42324727-42324746	None:intergenic	35.0%
	ATGTCTTCTCCGATCTTCTT+TGG	+	chr4.3:42324566-42324585	None:intergenic	40.0%
	GACGTAAACTTCGACGATTT+GGG	+	chr4.3:42324474-42324493	None:intergenic	40.0%
!	TAAACTTCGACGATTTGGGA+TGG	+	chr4.3:42324470-42324489	None:intergenic	40.0%
!	TGTTGATGGGAAAGATCCAA+AGG	-	chr4.3:42324877-42324896	MS.gene007662:CDS	40.0%
	AGATCCAAAGGATTTGCAGC+AGG	-	chr4.3:42324889-42324908	MS.gene007662:CDS	45.0%
	ATCTCCTGCTGCAAATCCTT+TGG	+	chr4.3:42324896-42324915	None:intergenic	45.0%
	CAAAATCTCCTTCACCGTTC+CGG	+	chr4.3:42324831-42324850	None:intergenic	45.0%
	CAAGGATTGGAAGGGTCTTA+GGG	-	chr4.3:42324601-42324620	MS.gene007662:CDS	45.0%
	CTGTTGGGTGTACTGTTGAT+GGG	-	chr4.3:42324864-42324883	MS.gene007662:CDS	45.0%
	CTTAGCCTGACGATTCTGAA+CGG	+	chr4.3:42324642-42324661	None:intergenic	45.0%
	GGACGTAAACTTCGACGATT+TGG	+	chr4.3:42324475-42324494	None:intergenic	45.0%
	GGTCTCTCACCAAAGAAGAT+CGG	-	chr4.3:42324554-42324573	MS.gene007662:CDS	45.0%
	TCTGTTGGGTGTACTGTTGA+TGG	-	chr4.3:42324863-42324882	MS.gene007662:CDS	45.0%
	TTGCAGCAGGAGATTAGTGA+TGG	-	chr4.3:42324902-42324921	MS.gene007662:CDS	45.0%
	TTGCGAAGATTATGAAGCCG+AGG	-	chr4.3:42324786-42324805	MS.gene007662:CDS	45.0%
!	ATTTGGGATGGATCGAGCTT+TGG	+	chr4.3:42324458-42324477	None:intergenic	45.0%
!	GAACGGTGAAGGAGATTTTG+GGG	-	chr4.3:42324831-42324850	MS.gene007662:CDS	45.0%
!	GCTGGTTATCAAGGCTTTGA+AGG	-	chr4.3:42324688-42324707	MS.gene007662:CDS	45.0%
!	GGAACGGTGAAGGAGATTTT+GGG	-	chr4.3:42324830-42324849	MS.gene007662:CDS	45.0%
!	TGGGGACTTGTGTTTCTGTT+GGG	-	chr4.3:42324849-42324868	MS.gene007662:CDS	45.0%
!	TGGTGATGTTGAGATTCCTC+AGG	-	chr4.3:42324922-42324941	MS.gene007662:CDS	45.0%
!	TTGGGGACTTGTGTTTCTGT+TGG	-	chr4.3:42324848-42324867	MS.gene007662:CDS	45.0%
!!	AGGCTTTGAAGGAACCAGAA+AGG	-	chr4.3:42324699-42324718	MS.gene007662:CDS	45.0%
!!	GGCTTTGAAGGAACCAGAAA+GGG	-	chr4.3:42324700-42324719	MS.gene007662:CDS	45.0%
	AAGACCCTTCCAATCCTTGG+CGG	+	chr4.3:42324600-42324619	None:intergenic	50.0%
	AGAAACCGCCAAGGATTGGA+AGG	-	chr4.3:42324592-42324611	MS.gene007662:CDS	50.0%
	CCAAAGAAACCGCCAAGGAT+TGG	-	chr4.3:42324588-42324607	MS.gene007662:CDS	50.0%
	CCAAGGATTGGAAGGGTCTT+AGG	-	chr4.3:42324600-42324619	MS.gene007662:CDS	50.0%
	CCAATCCTTGGCGGTTTCTT+TGG	+	chr4.3:42324591-42324610	None:intergenic	50.0%
	CCTAAGACCCTTCCAATCCT+TGG	+	chr4.3:42324603-42324622	None:intergenic	50.0%
	CGTTCAGAATCGTCAGGCTA+AGG	-	chr4.3:42324640-42324659	MS.gene007662:CDS	50.0%
	GAAACCGCCAAGGATTGGAA+GGG	-	chr4.3:42324593-42324612	MS.gene007662:CDS	50.0%
	GATTATGAAGCCGAGGTCGA+TGG	-	chr4.3:42324793-42324812	MS.gene007662:CDS	50.0%
	TTCTTTGGTGAGAGACCGAG+AGG	+	chr4.3:42324551-42324570	None:intergenic	50.0%
!	CGGAACGGTGAAGGAGATTT+TGG	-	chr4.3:42324829-42324848	MS.gene007662:CDS	50.0%
	AGACCGAGAGGACCGATCTT+GGG	+	chr4.3:42324539-42324558	None:intergenic	55.0%
	AGTTCCCTCGCTCCCAAGAT+CGG	-	chr4.3:42324524-42324543	MS.gene007662:CDS	55.0%
	CATCGCCAAAGAAACCGCCA+AGG	-	chr4.3:42324583-42324602	MS.gene007662:CDS	55.0%
	GAAGTTTACGTCCGCGTCAC+CGG	-	chr4.3:42324482-42324501	MS.gene007662:CDS	55.0%
	GCTCACCGTTCAGAATCGTC+AGG	-	chr4.3:42324634-42324653	MS.gene007662:CDS	55.0%
	GTCTTCTTGCGGTCCCTTTC+TGG	+	chr4.3:42324716-42324735	None:intergenic	55.0%
!	TGGGATGGATCGAGCTTTGG+CGG	+	chr4.3:42324455-42324474	None:intergenic	55.0%
	AGCTCCTTCGCCATCGACCT+CGG	+	chr4.3:42324806-42324825	None:intergenic	60.0%
	AGGACCGATCTTGGGAGCGA+GGG	+	chr4.3:42324531-42324550	None:intergenic	60.0%
	GAGACCGAGAGGACCGATCT+TGG	+	chr4.3:42324540-42324559	None:intergenic	60.0%
	GATCTTGGGAGCGAGGGAAC+TGG	+	chr4.3:42324525-42324544	None:intergenic	60.0%
	GCTCCCAAGATCGGTCCTCT+CGG	-	chr4.3:42324533-42324552	MS.gene007662:CDS	60.0%
	GGTCGCTGTTGTTCCGTCTG+CGG	-	chr4.3:42324661-42324680	MS.gene007662:CDS	60.0%
	TCGATGGCGAAGGAGCTTGC+CGG	-	chr4.3:42324809-42324828	MS.gene007662:CDS	60.0%
	ATAACCAGCGCCGCCGCAGA+CGG	+	chr4.3:42324677-42324696	None:intergenic	65.0%
	CGCTGTTGTTCCGTCTGCGG+CGG	-	chr4.3:42324664-42324683	MS.gene007662:CDS	65.0%
	GAAGCCGAGGTCGATGGCGA+AGG	-	chr4.3:42324799-42324818	MS.gene007662:CDS	65.0%
	GAGGACCGATCTTGGGAGCG+AGG	+	chr4.3:42324532-42324551	None:intergenic	65.0%
	GGAGCTTGCCGGAACGGTGA+AGG	-	chr4.3:42324820-42324839	MS.gene007662:CDS	65.0%
	GGCGAAGGAGCTTGCCGGAA+CGG	-	chr4.3:42324814-42324833	MS.gene007662:CDS	65.0%
	GTTTACGTCCGCGTCACCGG+CGG	-	chr4.3:42324485-42324504	MS.gene007662:CDS	65.0%
!!	TGCGGCGGCGCTGGTTATCA+AGG	-	chr4.3:42324679-42324698	MS.gene007662:CDS	65.0%
	CGACTTCACCGCCGGTGACG+CGG	+	chr4.3:42324496-42324515	None:intergenic	70.0%
	CGCGTCACCGGCGGTGAAGT+CGG	-	chr4.3:42324494-42324513	MS.gene007662:CDS	70.0%
	GGCAGCTCCGACTTCACCGC+CGG	+	chr4.3:42324504-42324523	None:intergenic	70.0%
	TGTTCCGTCTGCGGCGGCGC+TGG	-	chr4.3:42324670-42324689	MS.gene007662:CDS	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.3	gene	42324449	42324949	42324449	ID=MS.gene007662
chr4.3	mRNA	42324449	42324949	42324449	ID=MS.gene007662.t1;Parent=MS.gene007662
chr4.3	exon	42324449	42324949	42324449	ID=MS.gene007662.t1.exon1;Parent=MS.gene007662.t1
chr4.3	CDS	42324449	42324949	42324449	ID=cds.MS.gene007662.t1;Parent=MS.gene007662.t1

Gene Sequence

>MS.gene007662

ATGCCGCCAAAGCTCGATCCATCCCAAATCGTCGAAGTTTACGTCCGCGTCACCGGCGGTGAAGTCGGAGCTGCCAGTTCCCTCGCTCCCAAGATCGGTCCTCTCGGTCTCTCACCAAAGAAGATCGGAGAAGACATCGCCAAAGAAACCGCCAAGGATTGGAAGGGTCTTAGGGTGACAGTCAAGCTCACCGTTCAGAATCGTCAGGCTAAGGTCGCTGTTGTTCCGTCTGCGGCGGCGCTGGTTATCAAGGCTTTGAAGGAACCAGAAAGGGACCGCAAGAAGACTAAGAACATCAAGCATAATGGGAATATCTCTCTTGATGATGTTATTGAGATTGCGAAGATTATGAAGCCGAGGTCGATGGCGAAGGAGCTTGCCGGAACGGTGAAGGAGATTTTGGGGACTTGTGTTTCTGTTGGGTGTACTGTTGATGGGAAAGATCCAAAGGATTTGCAGCAGGAGATTAGTGATGGTGATGTTGAGATTCCTCAGGATTGA

Protein sequence

>MS.gene007662.t1

MPPKLDPSQIVEVYVRVTGGEVGAASSLAPKIGPLGLSPKKIGEDIAKETAKDWKGLRVTVKLTVQNRQAKVAVVPSAAALVIKALKEPERDRKKTKNIKHNGNISLDDVIEIAKIMKPRSMAKELAGTVKEILGTCVSVGCTVDGKDPKDLQQEISDGDVEIPQD