AlfalfaGEDB | MS.gene45417

M. sativa cultivar XinJiangDaYe / MS.gene45417

KEGG: K02870
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0019538 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576
PFAM: PF00298.22 PF03946.17

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene45417.t1	XP_013468200.1	100	166	0	0	1	166	1	166	2.10E-84	321.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene45417.t1	O50003	93.4	166	11	0	1	166	1	166	9.3e-81	300.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene45417.t1	I3SXZ6	100.0	166	0	0	1	166	1	166	1.5e-84	321.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene066163	MS.gene45417	PPI
MS.gene45417	MS.gene63721	PPI
MS.gene040581	MS.gene45417	PPI
MS.gene007661	MS.gene45417	PPI
MS.gene45417	MS.gene24598	PPI
MS.gene45417	MS.gene99744	PPI
MS.gene45417	MS.gene067072	PPI
MS.gene45417	MS.gene75537	PPI
MS.gene45417	MS.gene050134	PPI
MS.gene08871	MS.gene45417	PPI
MS.gene08870	MS.gene45417	PPI
MS.gene45417	MS.gene029242	PPI
MS.gene45417	MS.gene045180	PPI
MS.gene45417	MS.gene060216	PPI
MS.gene45417	MS.gene41916	PPI
MS.gene45417	MS.gene001986	PPI
MS.gene45417	MS.gene007662	PPI
MS.gene45417	MS.gene063366	PPI
MS.gene059422	MS.gene45417	PPI
MS.gene45417	MS.gene029840	PPI
MS.gene08636	MS.gene45417	PPI
MS.gene037324	MS.gene45417	PPI
MS.gene45417	MS.gene054186	PPI
MS.gene45417	MS.gene056257	PPI
MS.gene45417	MS.gene039110	PPI

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene45417.t1	MTR_1g064500	100.000	166	0	1	166	1	166	1.17e-116	327
MS.gene45417.t1	MTR_4g059400	99.390	164	1	1	164	1	164	1.21e-114	322
MS.gene45417.t1	MTR_2g094300	83.537	164	27	1	164	1	164	1.22e-97	279

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene45417.t1	AT5G60670	90.964	166	15	1	166	1	166	1.27e-107	304
MS.gene45417.t1	AT2G37190	90.854	164	15	1	164	1	164	5.76e-107	303
MS.gene45417.t1	AT3G53430	90.244	164	16	1	164	1	164	1.10e-106	302

Find 56 sgRNAs with CRISPR-Local

Find 58 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CAACCTTCGCTTGACGATTC+TGG	0.204840	1.1:-48748186	None:intergenic
ATGTCTTCTCCGATCTTCTT+TGG	0.275399	1.1:-48748106	None:intergenic
CGCGGCGGCGCTGGTGATTA+AGG	0.283872	1.1:+48748222	MS.gene45417:CDS
ATCTCTTGTTGCAAATCCTT+TGG	0.305493	1.1:-48748436	None:intergenic
GATTATGAAGCCGAGATCTA+TGG	0.306495	1.1:+48748336	MS.gene45417:CDS
TCTGTTGGTTGTACTGTTGA+TGG	0.359180	1.1:+48748406	MS.gene45417:CDS
CGAAGAATATCAAGCATAAT+GGG	0.367098	1.1:+48748278	MS.gene45417:CDS
ACGAAGAATATCAAGCATAA+TGG	0.368118	1.1:+48748277	MS.gene45417:CDS
ATCTGTGATGGGTCTAGCTT+TGG	0.382802	1.1:-48747998	None:intergenic
AGTTCCCTCGCTCCCAAGAT+TGG	0.390849	1.1:+48748067	MS.gene45417:CDS
CTTGGGACTTGTGTTTCTGT+TGG	0.402938	1.1:+48748391	MS.gene45417:CDS
TGAAGGAACCAGAGAGAGAT+AGG	0.404063	1.1:+48748248	MS.gene45417:CDS
GAGACCGAGGGGACCAATCT+TGG	0.405226	1.1:-48748080	None:intergenic
TGTTCCCTCCGCGGCGGCGC+TGG	0.411312	1.1:+48748213	MS.gene45417:CDS
GTCTTCTTCCTATCTCTCTC+TGG	0.414689	1.1:-48748256	None:intergenic
CCAAAGAAACCGCAAAAGAC+TGG	0.428144	1.1:+48748131	MS.gene45417:CDS
GGTACTGTGAAGGAGATTCT+TGG	0.433544	1.1:+48748373	MS.gene45417:CDS
AATCTTGGGAGCGAGGGAAC+TGG	0.439973	1.1:-48748065	None:intergenic
TGTGATGGGTCTAGCTTTGG+AGG	0.447457	1.1:-48747995	None:intergenic
AGACCGAGGGGACCAATCTT+GGG	0.459920	1.1:-48748079	None:intergenic
TCTATGGCGAAGGAGCTTGC+GGG	0.471180	1.1:+48748352	MS.gene45417:CDS
CTGTTGGTTGTACTGTTGAT+GGG	0.483928	1.1:+48748407	MS.gene45417:CDS
GAAGTCTACGTCCGCGTCAC+CGG	0.490300	1.1:+48748025	MS.gene45417:CDS
GGCGGCGCCGACTTCACCTC+CGG	0.496419	1.1:-48748044	None:intergenic
GTACTGTGAAGGAGATTCTT+GGG	0.502057	1.1:+48748374	MS.gene45417:CDS
ATCTATGGCGAAGGAGCTTG+CGG	0.510579	1.1:+48748351	MS.gene45417:CDS
GCTGGTGATTAAGGCGTTGA+AGG	0.514616	1.1:+48748231	MS.gene45417:CDS
CGCGTCACCGGAGGTGAAGT+CGG	0.523581	1.1:+48748037	MS.gene45417:CDS
GCTCCCAAGATTGGTCCCCT+CGG	0.529818	1.1:+48748076	MS.gene45417:CDS
GGGACCAATCTTGGGAGCGA+GGG	0.530376	1.1:-48748071	None:intergenic
AGCTCCTTCGCCATAGATCT+CGG	0.544258	1.1:-48748346	None:intergenic
GGGGACCAATCTTGGGAGCG+AGG	0.544731	1.1:-48748072	None:intergenic
ATCACCAGCGCCGCCGCGGA+GGG	0.551663	1.1:-48748217	None:intergenic
TTGCAACAAGAGATTGATGA+TGG	0.571171	1.1:+48748445	MS.gene45417:CDS
AATCACCAGCGCCGCCGCGG+AGG	0.577978	1.1:-48748218	None:intergenic
GGTCTCTCTCCAAAGAAGAT+CGG	0.584278	1.1:+48748097	MS.gene45417:CDS
CTTGGGAGCGAGGGAACTGG+CGG	0.586450	1.1:-48748062	None:intergenic
TAGACTTCGACGATCTGTGA+TGG	0.587094	1.1:-48748010	None:intergenic
CGACTTCACCTCCGGTGACG+CGG	0.588865	1.1:-48748036	None:intergenic
AGAAACCGCAAAAGACTGGA+AGG	0.591239	1.1:+48748135	MS.gene45417:CDS
CTTCTTTGGAGAGAGACCGA+GGG	0.594178	1.1:-48748092	None:intergenic
GGAGCTTGCGGGTACTGTGA+AGG	0.597173	1.1:+48748363	MS.gene45417:CDS
AGACTTCGACGATCTGTGAT+GGG	0.603678	1.1:-48748009	None:intergenic
TGCGGTTGTTCCCTCCGCGG+CGG	0.605465	1.1:+48748207	MS.gene45417:CDS
GTCTACGTCCGCGTCACCGG+AGG	0.608763	1.1:+48748028	MS.gene45417:CDS
CTTAATCACCAGCGCCGCCG+CGG	0.610925	1.1:-48748221	None:intergenic
TCTTCTTTGGAGAGAGACCG+AGG	0.616068	1.1:-48748093	None:intergenic
GAATCGTCAAGCGAAGGTTG+CGG	0.619107	1.1:+48748189	MS.gene45417:CDS
TGTTGATGGGAAAGATCCAA+AGG	0.621231	1.1:+48748420	MS.gene45417:CDS
CTTCGCTTGACGATTCTGGA+CGG	0.629414	1.1:-48748182	None:intergenic
CGTCCAGAATCGTCAAGCGA+AGG	0.633297	1.1:+48748183	MS.gene45417:CDS
GAAGCCGAGATCTATGGCGA+AGG	0.651418	1.1:+48748342	MS.gene45417:CDS
GAAACCGCAAAAGACTGGAA+GGG	0.670069	1.1:+48748136	MS.gene45417:CDS
GGTTGCGGTTGTTCCCTCCG+CGG	0.676784	1.1:+48748204	MS.gene45417:CDS
TTCTTTGGAGAGAGACCGAG+GGG	0.684696	1.1:-48748091	None:intergenic
CGGTGAGCTTGACTGTGACA+CGG	0.697095	1.1:-48748162	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	ACGAAGAATATCAAGCATAA+TGG	+	chr1.1:48748277-48748296	MS.gene45417:CDS	30.0%
	CGAAGAATATCAAGCATAAT+GGG	+	chr1.1:48748278-48748297	MS.gene45417:CDS	30.0%
	ATCTCTTGTTGCAAATCCTT+TGG	-	chr1.1:48748439-48748458	None:intergenic	35.0%
	TTGCAACAAGAGATTGATGA+TGG	+	chr1.1:48748445-48748464	MS.gene45417:CDS	35.0%
	ATGTCTTCTCCGATCTTCTT+TGG	-	chr1.1:48748109-48748128	None:intergenic	40.0%
	GATTATGAAGCCGAGATCTA+TGG	+	chr1.1:48748336-48748355	MS.gene45417:CDS	40.0%
	GTACTGTGAAGGAGATTCTT+GGG	+	chr1.1:48748374-48748393	MS.gene45417:CDS	40.0%
!	CTGTTGGTTGTACTGTTGAT+GGG	+	chr1.1:48748407-48748426	MS.gene45417:CDS	40.0%
!	TCTGTTGGTTGTACTGTTGA+TGG	+	chr1.1:48748406-48748425	MS.gene45417:CDS	40.0%
!	TGTTGATGGGAAAGATCCAA+AGG	+	chr1.1:48748420-48748439	MS.gene45417:CDS	40.0%
	AGAAACCGCAAAAGACTGGA+AGG	+	chr1.1:48748135-48748154	MS.gene45417:CDS	45.0%
	AGACTTCGACGATCTGTGAT+GGG	-	chr1.1:48748012-48748031	None:intergenic	45.0%
	CCAAAGAAACCGCAAAAGAC+TGG	+	chr1.1:48748131-48748150	MS.gene45417:CDS	45.0%
	GAAACCGCAAAAGACTGGAA+GGG	+	chr1.1:48748136-48748155	MS.gene45417:CDS	45.0%
	GGTACTGTGAAGGAGATTCT+TGG	+	chr1.1:48748373-48748392	MS.gene45417:CDS	45.0%
	GGTCTCTCTCCAAAGAAGAT+CGG	+	chr1.1:48748097-48748116	MS.gene45417:CDS	45.0%
	GTCTTCTTCCTATCTCTCTC+TGG	-	chr1.1:48748259-48748278	None:intergenic	45.0%
	TAGACTTCGACGATCTGTGA+TGG	-	chr1.1:48748013-48748032	None:intergenic	45.0%
	TGAAGGAACCAGAGAGAGAT+AGG	+	chr1.1:48748248-48748267	MS.gene45417:CDS	45.0%
!	CCAGTCTTTTGCGGTTTCTT+TGG	-	chr1.1:48748134-48748153	None:intergenic	45.0%
!	CTTGGGACTTGTGTTTCTGT+TGG	+	chr1.1:48748391-48748410	MS.gene45417:CDS	45.0%
!!	ATCTGTGATGGGTCTAGCTT+TGG	-	chr1.1:48748001-48748020	None:intergenic	45.0%
	AGCTCCTTCGCCATAGATCT+CGG	-	chr1.1:48748349-48748368	None:intergenic	50.0%
	ATCTATGGCGAAGGAGCTTG+CGG	+	chr1.1:48748351-48748370	MS.gene45417:CDS	50.0%
	CAACCTTCGCTTGACGATTC+TGG	-	chr1.1:48748189-48748208	None:intergenic	50.0%
	CTTCGCTTGACGATTCTGGA+CGG	-	chr1.1:48748185-48748204	None:intergenic	50.0%
	GAATCGTCAAGCGAAGGTTG+CGG	+	chr1.1:48748189-48748208	MS.gene45417:CDS	50.0%
!	CTTCTTTGGAGAGAGACCGA+GGG	-	chr1.1:48748095-48748114	None:intergenic	50.0%
!	GAGACCCTTCCAGTCTTTTG+CGG	-	chr1.1:48748143-48748162	None:intergenic	50.0%
!	TCTTCTTTGGAGAGAGACCG+AGG	-	chr1.1:48748096-48748115	None:intergenic	50.0%
!	TTCTTTGGAGAGAGACCGAG+GGG	-	chr1.1:48748094-48748113	None:intergenic	50.0%
!!	GCTGGTGATTAAGGCGTTGA+AGG	+	chr1.1:48748231-48748250	MS.gene45417:CDS	50.0%
!!	TGTGATGGGTCTAGCTTTGG+AGG	-	chr1.1:48747998-48748017	None:intergenic	50.0%
	AATCTTGGGAGCGAGGGAAC+TGG	-	chr1.1:48748068-48748087	None:intergenic	55.0%
	AGACCGAGGGGACCAATCTT+GGG	-	chr1.1:48748082-48748101	None:intergenic	55.0%
	AGTTCCCTCGCTCCCAAGAT+TGG	+	chr1.1:48748067-48748086	MS.gene45417:CDS	55.0%
	CGGTGAGCTTGACTGTGACA+CGG	-	chr1.1:48748165-48748184	None:intergenic	55.0%
	CGTCCAGAATCGTCAAGCGA+AGG	+	chr1.1:48748183-48748202	MS.gene45417:CDS	55.0%
	GAAGCCGAGATCTATGGCGA+AGG	+	chr1.1:48748342-48748361	MS.gene45417:CDS	55.0%
	TCTATGGCGAAGGAGCTTGC+GGG	+	chr1.1:48748352-48748371	MS.gene45417:CDS	55.0%
	GAAGTCTACGTCCGCGTCAC+CGG	+	chr1.1:48748025-48748044	MS.gene45417:CDS	60.0%
	GAGACCGAGGGGACCAATCT+TGG	-	chr1.1:48748083-48748102	None:intergenic	60.0%
	GGGACCAATCTTGGGAGCGA+GGG	-	chr1.1:48748074-48748093	None:intergenic	60.0%
!	GCTCCCAAGATTGGTCCCCT+CGG	+	chr1.1:48748076-48748095	MS.gene45417:CDS	60.0%
!	GGAGCTTGCGGGTACTGTGA+AGG	+	chr1.1:48748363-48748382	MS.gene45417:CDS	60.0%
	CGACTTCACCTCCGGTGACG+CGG	-	chr1.1:48748039-48748058	None:intergenic	65.0%
	CGCGTCACCGGAGGTGAAGT+CGG	+	chr1.1:48748037-48748056	MS.gene45417:CDS	65.0%
	CTTAATCACCAGCGCCGCCG+CGG	-	chr1.1:48748224-48748243	None:intergenic	65.0%
	CTTGGGAGCGAGGGAACTGG+CGG	-	chr1.1:48748065-48748084	None:intergenic	65.0%
	GGGGACCAATCTTGGGAGCG+AGG	-	chr1.1:48748075-48748094	None:intergenic	65.0%
	GGTTGCGGTTGTTCCCTCCG+CGG	+	chr1.1:48748204-48748223	MS.gene45417:CDS	65.0%
	GTCTACGTCCGCGTCACCGG+AGG	+	chr1.1:48748028-48748047	MS.gene45417:CDS	70.0%
	TGCGGTTGTTCCCTCCGCGG+CGG	+	chr1.1:48748207-48748226	MS.gene45417:CDS	70.0%
!!	CGCGGCGGCGCTGGTGATTA+AGG	+	chr1.1:48748222-48748241	MS.gene45417:CDS	70.0%
	AATCACCAGCGCCGCCGCGG+AGG	-	chr1.1:48748221-48748240	None:intergenic	75.0%
	ATCACCAGCGCCGCCGCGGA+GGG	-	chr1.1:48748220-48748239	None:intergenic	75.0%
!!	GGCGGCGCCGACTTCACCTC+CGG	-	chr1.1:48748047-48748066	None:intergenic	75.0%
!	TGTTCCCTCCGCGGCGGCGC+TGG	+	chr1.1:48748213-48748232	MS.gene45417:CDS	80.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.1	gene	48747992	48748492	48747992	ID=MS.gene45417
chr1.1	mRNA	48747992	48748492	48747992	ID=MS.gene45417.t1;Parent=MS.gene45417
chr1.1	exon	48747992	48748492	48747992	ID=MS.gene45417.t1.exon1;Parent=MS.gene45417.t1
chr1.1	CDS	48747992	48748492	48747992	ID=cds.MS.gene45417.t1;Parent=MS.gene45417.t1

Gene Sequence

>MS.gene45417

ATGCCTCCAAAGCTAGACCCATCACAGATCGTCGAAGTCTACGTCCGCGTCACCGGAGGTGAAGTCGGCGCCGCCAGTTCCCTCGCTCCCAAGATTGGTCCCCTCGGTCTCTCTCCAAAGAAGATCGGAGAAGACATCGCCAAAGAAACCGCAAAAGACTGGAAGGGTCTCCGTGTCACAGTCAAGCTCACCGTCCAGAATCGTCAAGCGAAGGTTGCGGTTGTTCCCTCCGCGGCGGCGCTGGTGATTAAGGCGTTGAAGGAACCAGAGAGAGATAGGAAGAAGACGAAGAATATCAAGCATAATGGGAATATCTCGCTTGATGATGTTATTGAGATTGCGAAGATTATGAAGCCGAGATCTATGGCGAAGGAGCTTGCGGGTACTGTGAAGGAGATTCTTGGGACTTGTGTTTCTGTTGGTTGTACTGTTGATGGGAAAGATCCAAAGGATTTGCAACAAGAGATTGATGATGGTGATGTTGAGATTCCTCTAGAATAA

Protein sequence

>MS.gene45417.t1

MPPKLDPSQIVEVYVRVTGGEVGAASSLAPKIGPLGLSPKKIGEDIAKETAKDWKGLRVTVKLTVQNRQAKVAVVPSAAALVIKALKEPERDRKKTKNIKHNGNISLDDVIEIAKIMKPRSMAKELAGTVKEILGTCVSVGCTVDGKDPKDLQQEIDDGDVEIPLE