AlfalfaGEDB | MS.gene08636

M. sativa cultivar XinJiangDaYe / MS.gene08636

KEGG: K02870
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0019538 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576
PFAM: PF00298.22 PF03946.17

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene08636.t1	XP_003606385.1	99.4	166	1	0	1	166	1	166	8.00E-84	319.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene08636.t1	O50003	93.4	166	11	0	1	166	1	166	1.2e-80	300.4

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene08636.t1	B7FH91	99.4	166	1	0	1	166	1	166	5.8e-84	319.7

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049306	MS.gene08636	0.819098	1.39E-52	-1.69E-46
MS.gene051925	MS.gene08636	0.840715	7.36E-58	-1.69E-46
MS.gene051927	MS.gene08636	0.830724	2.50E-55	-1.69E-46
MS.gene057321	MS.gene08636	0.818614	1.79E-52	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene08636	MS.gene037324	PPI
MS.gene08636	MS.gene054186	PPI
MS.gene08636	MS.gene056257	PPI
MS.gene08870	MS.gene08636	PPI
MS.gene08636	MS.gene24598	PPI
MS.gene08636	MS.gene99744	PPI
MS.gene08636	MS.gene067072	PPI
MS.gene08871	MS.gene08636	PPI
MS.gene08636	MS.gene029242	PPI
MS.gene08636	MS.gene75537	PPI
MS.gene08636	MS.gene050134	PPI
MS.gene08636	MS.gene045180	PPI
MS.gene08636	MS.gene007661	PPI
MS.gene059422	MS.gene08636	PPI
MS.gene08636	MS.gene001986	PPI
MS.gene08636	MS.gene007662	PPI
MS.gene08636	MS.gene063366	PPI
MS.gene08636	MS.gene060216	PPI
MS.gene08636	MS.gene029840	PPI
MS.gene08636	MS.gene41916	PPI
MS.gene066163	MS.gene08636	PPI
MS.gene040581	MS.gene08636	PPI
MS.gene08636	MS.gene45417	PPI
MS.gene007661	MS.gene08636	PPI
MS.gene08636	MS.gene039110	PPI
MS.gene08636	MS.gene08871	PPI
MS.gene08636	MS.gene63721	PPI

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene08636.t1	MTR_4g059400	99.398	166	1	1	166	1	166	1.89e-116	327
MS.gene08636.t1	MTR_1g064500	99.390	164	1	1	164	1	164	2.24e-114	321
MS.gene08636.t1	MTR_2g094300	83.537	164	27	1	164	1	164	1.50e-97	279

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene08636.t1	AT5G60670	90.361	166	16	1	166	1	166	9.53e-108	305
MS.gene08636.t1	AT2G37190	89.759	166	17	1	166	1	166	1.20e-107	304
MS.gene08636.t1	AT3G53430	89.157	166	18	1	166	1	166	2.70e-107	303

Find 65 sgRNAs with CRISPR-Local

Find 64 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GTCTTCTTGCGGTCCCTTTC+TGG	0.085933	4.1:+39250235	None:intergenic
CGGAACTGTGAAGGAGATTT+TGG	0.121001	4.1:-39250119	MS.gene08636:CDS
GGACGTAAACTTCGACGATT+TGG	0.184989	4.1:+39250476	None:intergenic
CCAATCCTTGGCGGTTTCTT+TGG	0.201986	4.1:+39250360	None:intergenic
ATGTCTTCTCCGATCTTCTT+TGG	0.296410	4.1:+39250385	None:intergenic
ACTAAGAACATCAAGCATAA+TGG	0.336453	4.1:-39250214	MS.gene08636:CDS
TCTGTTGGGTGTACTGTTGA+TGG	0.361476	4.1:-39250085	MS.gene08636:CDS
CTAAGAACATCAAGCATAAT+GGG	0.363010	4.1:-39250213	MS.gene08636:CDS
GACGTAAACTTCGACGATTT+GGG	0.369524	4.1:+39250477	None:intergenic
CAAAATCTCCTTCACAGTTC+CGG	0.393278	4.1:+39250120	None:intergenic
ATCTCCTGCTGCAAATCCTT+TGG	0.401059	4.1:+39250055	None:intergenic
TTGGGTACTTGTGTTTCTGT+TGG	0.418354	4.1:-39250100	MS.gene08636:CDS
ATTTGGGATGGATCGAGCTT+TGG	0.421723	4.1:+39250493	None:intergenic
TGCGGCGGCGCTGGTTATCA+AGG	0.430495	4.1:-39250269	MS.gene08636:CDS
TGGGTACTTGTGTTTCTGTT+GGG	0.442622	4.1:-39250099	MS.gene08636:CDS
CCAAAGAAACCGCCAAGGAT+TGG	0.444226	4.1:-39250360	MS.gene08636:CDS
GATCTTGGGAGCGAGGGAAC+TGG	0.450092	4.1:+39250426	None:intergenic
TGTTCCGTCTGCGGCGGCGC+TGG	0.455070	4.1:-39250278	MS.gene08636:CDS
CAGACCCTTCCAATCCTTGG+CGG	0.455684	4.1:+39250351	None:intergenic
GAGACCGAGAGGACCGATCT+TGG	0.459185	4.1:+39250411	None:intergenic
TCCATGGCGAAGGAGCTTGC+CGG	0.471094	4.1:-39250139	MS.gene08636:CDS
GGCTTTGAAGGAACCAGAAA+GGG	0.473237	4.1:-39250248	MS.gene08636:CDS
CCTCAGACCCTTCCAATCCT+TGG	0.473920	4.1:+39250348	None:intergenic
GCTGGTTATCAAGGCTTTGA+AGG	0.478691	4.1:-39250260	MS.gene08636:CDS
AGACCGAGAGGACCGATCTT+GGG	0.482147	4.1:+39250412	None:intergenic
GATTATGAAGCCGAGGTCCA+TGG	0.484809	4.1:-39250155	MS.gene08636:CDS
AGTTCCCTCGCTCCCAAGAT+CGG	0.497799	4.1:-39250424	MS.gene08636:CDS
GAAGTTTACGTCCGTGTCAC+CGG	0.499320	4.1:-39250466	MS.gene08636:CDS
CTGTTGGGTGTACTGTTGAT+GGG	0.503488	4.1:-39250084	MS.gene08636:CDS
TGGGATGGATCGAGCTTTGG+CGG	0.507491	4.1:+39250496	None:intergenic
CGTGTCACCGGCGGTGAAGT+CGG	0.509886	4.1:-39250454	MS.gene08636:CDS
AGCTCCTTCGCCATGGACCT+CGG	0.515558	4.1:+39250145	None:intergenic
CGCTGTTGTTCCGTCTGCGG+CGG	0.521792	4.1:-39250284	MS.gene08636:CDS
CCAAGGATTGGAAGGGTCTG+AGG	0.527691	4.1:-39250348	MS.gene08636:CDS
GCTCCCAAGATCGGTCCTCT+CGG	0.529023	4.1:-39250415	MS.gene08636:CDS
GGCGGCTCCGACTTCACCGC+CGG	0.532286	4.1:+39250447	None:intergenic
GTTGAGATTCCTCAGGATTG+AGG	0.534021	4.1:-39250019	None:intergenic
CGACTTCACCGCCGGTGACA+CGG	0.537897	4.1:+39250455	None:intergenic
AGAAACCGCCAAGGATTGGA+AGG	0.540852	4.1:-39250356	MS.gene08636:CDS
CGTTCAGAATCGTCAGGCTA+AGG	0.541896	4.1:-39250308	MS.gene08636:CDS
TCCGGCAAGCTCCTTCGCCA+TGG	0.545168	4.1:+39250138	None:intergenic
GCTCACCGTTCAGAATCGTC+AGG	0.553915	4.1:-39250314	MS.gene08636:CDS
AGATCCAAAGGATTTGCAGC+AGG	0.556469	4.1:-39250059	MS.gene08636:CDS
AGGCTTTGAAGGAACCAGAA+AGG	0.556759	4.1:-39250249	MS.gene08636:CDS
TGATGTTCTTAGTCTTCTTG+CGG	0.558436	4.1:+39250224	None:intergenic
AGGACCGATCTTGGGAGCGA+GGG	0.558873	4.1:+39250420	None:intergenic
TGGTGATGTTGAGATTCCTC+AGG	0.564181	4.1:-39250026	MS.gene08636:CDS
GAGGACCGATCTTGGGAGCG+AGG	0.581430	4.1:+39250419	None:intergenic
GGAGCTTGCCGGAACTGTGA+AGG	0.582805	4.1:-39250128	MS.gene08636:CDS
GAAGCCGAGGTCCATGGCGA+AGG	0.584975	4.1:-39250149	MS.gene08636:CDS
GGTCTCTCACCAAAGAAGAT+CGG	0.586947	4.1:-39250394	MS.gene08636:CDS
ATAACCAGCGCCGCCGCAGA+CGG	0.589868	4.1:+39250274	None:intergenic
TTGCAGCAGGAGATTAGTGA+TGG	0.592966	4.1:-39250046	MS.gene08636:CDS
TAAACTTCGACGATTTGGGA+TGG	0.609008	4.1:+39250481	None:intergenic
CAAGGATTGGAAGGGTCTGA+GGG	0.614585	4.1:-39250347	MS.gene08636:CDS
TGTTGATGGGAAAGATCCAA+AGG	0.621231	4.1:-39250071	MS.gene08636:CDS
CTTGGGAGCGAGGGAACTGG+CGG	0.624967	4.1:+39250429	None:intergenic
GGTCGCTGTTGTTCCGTCTG+CGG	0.635590	4.1:-39250287	MS.gene08636:CDS
GTTTACGTCCGTGTCACCGG+CGG	0.651197	4.1:-39250463	MS.gene08636:CDS
TTGCGAAGATTATGAAGCCG+AGG	0.656123	4.1:-39250162	MS.gene08636:CDS
TTCTTTGGTGAGAGACCGAG+AGG	0.661014	4.1:+39250400	None:intergenic
CTTAGCCTGACGATTCTGAA+CGG	0.665775	4.1:+39250309	None:intergenic
GAAACCGCCAAGGATTGGAA+GGG	0.668035	4.1:-39250355	MS.gene08636:CDS
CAACAACAACCTCAATCCTG+AGG	0.669617	4.1:+39250010	None:intergenic
CATCGCCAAAGAAACCGCCA+AGG	0.687884	4.1:-39250365	MS.gene08636:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	ACTAAGAACATCAAGCATAA+TGG	-	chr4.1:39250306-39250325	MS.gene08636:CDS	30.0%
	CTAAGAACATCAAGCATAAT+GGG	-	chr4.1:39250307-39250326	MS.gene08636:CDS	30.0%
!	TGATGTTCTTAGTCTTCTTG+CGG	+	chr4.1:39250299-39250318	None:intergenic	35.0%
	ATGTCTTCTCCGATCTTCTT+TGG	+	chr4.1:39250138-39250157	None:intergenic	40.0%
	CAAAATCTCCTTCACAGTTC+CGG	+	chr4.1:39250403-39250422	None:intergenic	40.0%
	GACGTAAACTTCGACGATTT+GGG	+	chr4.1:39250046-39250065	None:intergenic	40.0%
!	GGAACTGTGAAGGAGATTTT+GGG	-	chr4.1:39250402-39250421	MS.gene08636:CDS	40.0%
!	TAAACTTCGACGATTTGGGA+TGG	+	chr4.1:39250042-39250061	None:intergenic	40.0%
!	TGGGTACTTGTGTTTCTGTT+GGG	-	chr4.1:39250421-39250440	MS.gene08636:CDS	40.0%
!	TGTTGATGGGAAAGATCCAA+AGG	-	chr4.1:39250449-39250468	MS.gene08636:CDS	40.0%
!	TTGGGTACTTGTGTTTCTGT+TGG	-	chr4.1:39250420-39250439	MS.gene08636:CDS	40.0%
	AGATCCAAAGGATTTGCAGC+AGG	-	chr4.1:39250461-39250480	MS.gene08636:CDS	45.0%
	ATCTCCTGCTGCAAATCCTT+TGG	+	chr4.1:39250468-39250487	None:intergenic	45.0%
	CTGTTGGGTGTACTGTTGAT+GGG	-	chr4.1:39250436-39250455	MS.gene08636:CDS	45.0%
	CTTAGCCTGACGATTCTGAA+CGG	+	chr4.1:39250214-39250233	None:intergenic	45.0%
	GGACGTAAACTTCGACGATT+TGG	+	chr4.1:39250047-39250066	None:intergenic	45.0%
	GGTCTCTCACCAAAGAAGAT+CGG	-	chr4.1:39250126-39250145	MS.gene08636:CDS	45.0%
	TCTGTTGGGTGTACTGTTGA+TGG	-	chr4.1:39250435-39250454	MS.gene08636:CDS	45.0%
	TTGCAGCAGGAGATTAGTGA+TGG	-	chr4.1:39250474-39250493	MS.gene08636:CDS	45.0%
	TTGCGAAGATTATGAAGCCG+AGG	-	chr4.1:39250358-39250377	MS.gene08636:CDS	45.0%
!	ATTTGGGATGGATCGAGCTT+TGG	+	chr4.1:39250030-39250049	None:intergenic	45.0%
!	CGGAACTGTGAAGGAGATTT+TGG	-	chr4.1:39250401-39250420	MS.gene08636:CDS	45.0%
!	GCTGGTTATCAAGGCTTTGA+AGG	-	chr4.1:39250260-39250279	MS.gene08636:CDS	45.0%
!	TGGTGATGTTGAGATTCCTC+AGG	-	chr4.1:39250494-39250513	MS.gene08636:CDS	45.0%
!!	AGGCTTTGAAGGAACCAGAA+AGG	-	chr4.1:39250271-39250290	MS.gene08636:CDS	45.0%
!!	GGCTTTGAAGGAACCAGAAA+GGG	-	chr4.1:39250272-39250291	MS.gene08636:CDS	45.0%
	AGAAACCGCCAAGGATTGGA+AGG	-	chr4.1:39250164-39250183	MS.gene08636:CDS	50.0%
	CAAGGATTGGAAGGGTCTGA+GGG	-	chr4.1:39250173-39250192	MS.gene08636:CDS	50.0%
	CCAAAGAAACCGCCAAGGAT+TGG	-	chr4.1:39250160-39250179	MS.gene08636:CDS	50.0%
	CCAATCCTTGGCGGTTTCTT+TGG	+	chr4.1:39250163-39250182	None:intergenic	50.0%
	CGTTCAGAATCGTCAGGCTA+AGG	-	chr4.1:39250212-39250231	MS.gene08636:CDS	50.0%
	GAAACCGCCAAGGATTGGAA+GGG	-	chr4.1:39250165-39250184	MS.gene08636:CDS	50.0%
	GAAGTTTACGTCCGTGTCAC+CGG	-	chr4.1:39250054-39250073	MS.gene08636:CDS	50.0%
	GATTATGAAGCCGAGGTCCA+TGG	-	chr4.1:39250365-39250384	MS.gene08636:CDS	50.0%
	TTCTTTGGTGAGAGACCGAG+AGG	+	chr4.1:39250123-39250142	None:intergenic	50.0%
	AGACCGAGAGGACCGATCTT+GGG	+	chr4.1:39250111-39250130	None:intergenic	55.0%
	AGTTCCCTCGCTCCCAAGAT+CGG	-	chr4.1:39250096-39250115	MS.gene08636:CDS	55.0%
	CAGACCCTTCCAATCCTTGG+CGG	+	chr4.1:39250172-39250191	None:intergenic	55.0%
	CATCGCCAAAGAAACCGCCA+AGG	-	chr4.1:39250155-39250174	MS.gene08636:CDS	55.0%
	CCAAGGATTGGAAGGGTCTG+AGG	-	chr4.1:39250172-39250191	MS.gene08636:CDS	55.0%
	CCTCAGACCCTTCCAATCCT+TGG	+	chr4.1:39250175-39250194	None:intergenic	55.0%
	GCTCACCGTTCAGAATCGTC+AGG	-	chr4.1:39250206-39250225	MS.gene08636:CDS	55.0%
	GTCTTCTTGCGGTCCCTTTC+TGG	+	chr4.1:39250288-39250307	None:intergenic	55.0%
!	TGGGATGGATCGAGCTTTGG+CGG	+	chr4.1:39250027-39250046	None:intergenic	55.0%
	AGCTCCTTCGCCATGGACCT+CGG	+	chr4.1:39250378-39250397	None:intergenic	60.0%
	AGGACCGATCTTGGGAGCGA+GGG	+	chr4.1:39250103-39250122	None:intergenic	60.0%
	GAGACCGAGAGGACCGATCT+TGG	+	chr4.1:39250112-39250131	None:intergenic	60.0%
	GATCTTGGGAGCGAGGGAAC+TGG	+	chr4.1:39250097-39250116	None:intergenic	60.0%
	GCTCCCAAGATCGGTCCTCT+CGG	-	chr4.1:39250105-39250124	MS.gene08636:CDS	60.0%
	GGAGCTTGCCGGAACTGTGA+AGG	-	chr4.1:39250392-39250411	MS.gene08636:CDS	60.0%
	GGTCGCTGTTGTTCCGTCTG+CGG	-	chr4.1:39250233-39250252	MS.gene08636:CDS	60.0%
	GTTTACGTCCGTGTCACCGG+CGG	-	chr4.1:39250057-39250076	MS.gene08636:CDS	60.0%
	TCCATGGCGAAGGAGCTTGC+CGG	-	chr4.1:39250381-39250400	MS.gene08636:CDS	60.0%
	ATAACCAGCGCCGCCGCAGA+CGG	+	chr4.1:39250249-39250268	None:intergenic	65.0%
	CGACTTCACCGCCGGTGACA+CGG	+	chr4.1:39250068-39250087	None:intergenic	65.0%
	CGCTGTTGTTCCGTCTGCGG+CGG	-	chr4.1:39250236-39250255	MS.gene08636:CDS	65.0%
	CGTGTCACCGGCGGTGAAGT+CGG	-	chr4.1:39250066-39250085	MS.gene08636:CDS	65.0%
	CTTGGGAGCGAGGGAACTGG+CGG	+	chr4.1:39250094-39250113	None:intergenic	65.0%
	GAAGCCGAGGTCCATGGCGA+AGG	-	chr4.1:39250371-39250390	MS.gene08636:CDS	65.0%
	GAGGACCGATCTTGGGAGCG+AGG	+	chr4.1:39250104-39250123	None:intergenic	65.0%
	TCCGGCAAGCTCCTTCGCCA+TGG	+	chr4.1:39250385-39250404	None:intergenic	65.0%
!!	TGCGGCGGCGCTGGTTATCA+AGG	-	chr4.1:39250251-39250270	MS.gene08636:CDS	65.0%
	GGCGGCTCCGACTTCACCGC+CGG	+	chr4.1:39250076-39250095	None:intergenic	75.0%
	TGTTCCGTCTGCGGCGGCGC+TGG	-	chr4.1:39250242-39250261	MS.gene08636:CDS	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.1	gene	39250021	39250521	39250021	ID=MS.gene08636
chr4.1	mRNA	39250021	39250521	39250021	ID=MS.gene08636.t1;Parent=MS.gene08636
chr4.1	exon	39250021	39250521	39250021	ID=MS.gene08636.t1.exon1;Parent=MS.gene08636.t1
chr4.1	CDS	39250021	39250521	39250021	ID=cds.MS.gene08636.t1;Parent=MS.gene08636.t1

Gene Sequence

>MS.gene08636

ATGCCGCCAAAGCTCGATCCATCCCAAATCGTCGAAGTTTACGTCCGTGTCACCGGCGGTGAAGTCGGAGCCGCCAGTTCCCTCGCTCCCAAGATCGGTCCTCTCGGTCTCTCACCAAAGAAGATCGGAGAAGACATCGCCAAAGAAACCGCCAAGGATTGGAAGGGTCTGAGGGTGACAGTCAAGCTCACCGTTCAGAATCGTCAGGCTAAGGTCGCTGTTGTTCCGTCTGCGGCGGCGCTGGTTATCAAGGCTTTGAAGGAACCAGAAAGGGACCGCAAGAAGACTAAGAACATCAAGCATAATGGGAATATCTCTCTTGATGATGTTATTGAGATTGCGAAGATTATGAAGCCGAGGTCCATGGCGAAGGAGCTTGCCGGAACTGTGAAGGAGATTTTGGGTACTTGTGTTTCTGTTGGGTGTACTGTTGATGGGAAAGATCCAAAGGATTTGCAGCAGGAGATTAGTGATGGTGATGTTGAGATTCCTCAGGATTGA

Protein sequence

>MS.gene08636.t1

MPPKLDPSQIVEVYVRVTGGEVGAASSLAPKIGPLGLSPKKIGEDIAKETAKDWKGLRVTVKLTVQNRQAKVAVVPSAAALVIKALKEPERDRKKTKNIKHNGNISLDDVIEIAKIMKPRSMAKELAGTVKEILGTCVSVGCTVDGKDPKDLQQEISDGDVEIPQD