AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene99744

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene99744.t1 MTR_1g114000 90.794 315 0 2 1 288 1 313 0.0 544
MS.gene99744.t1 MTR_6g047580 86.722 241 5 1 75 288 69 309 4.30e-149 420
MS.gene99744.t1 MTR_6g047580 84.211 266 15 1 50 288 12 277 1.29e-146 412
MS.gene99744.t1 MTR_6g047580 97.297 185 5 0 75 259 69 253 3.11e-130 370
MS.gene99744.t1 MTR_6g047580 84.737 190 27 1 75 264 69 256 2.93e-113 327
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene99744.t1 AT4G25630 82.377 244 16 1 71 287 74 317 5.54e-145 410
MS.gene99744.t1 AT5G52470 81.818 242 17 1 71 285 63 304 5.48e-144 407
MS.gene99744.t1 AT5G52490 65.702 242 56 1 69 283 51 292 8.87e-112 325
MS.gene99744.t1 AT5G52470 93.902 164 10 0 71 234 63 226 1.16e-111 324

Find 105 sgRNAs with CRISPR-Local

Find 200 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAAAATACCCTCATGTCTA+TGG 0.205671 1.2:+83989348 None:intergenic
GGAGGTGGGAGAGGTGGTTT+TGG 0.245696 1.2:-83989512 MS.gene99744:CDS
TGTCTAGCATCTTCAATAAT+TGG 0.265009 1.2:+83988507 None:intergenic
CTTTACCTAGGAGCTGCTTC+TGG 0.296675 1.2:-83988752 MS.gene99744:CDS
TATAACATTAGTACGCTTCT+TGG 0.324454 1.2:+83988530 None:intergenic
CTAAGACAGAGTATAGAATT+TGG 0.324854 1.2:-83988934 MS.gene99744:CDS
GGAGGAAGGGGTGGTGATAG+AGG 0.351363 1.2:-83989542 MS.gene99744:CDS
TGGGCGTGACTTGGTCAATA+TGG 0.352165 1.2:-83988553 MS.gene99744:CDS
TCAAGAGTCACTTGCTCCAT+TGG 0.352395 1.2:+83986973 None:intergenic
GGCCGTGGTGGTTCTGGTGG+AGG 0.363670 1.2:-83989572 MS.gene99744:CDS
TGTACAGGCCGTGGTGGTTC+TGG 0.364877 1.2:-83989578 MS.gene99744:intron
GGAGGCCGTGGTGGCGGAAG+AGG 0.374341 1.2:-83989410 MS.gene99744:CDS
GGAGGTAGAGGAGGTGGGAG+AGG 0.378171 1.2:-83989521 MS.gene99744:CDS
GATAGAGGAGGTAGAGGAGG+TGG 0.391157 1.2:-83989527 MS.gene99744:CDS
CGGAAGAGGTGGTGGAATGA+AGG 0.392714 1.2:-83989396 MS.gene99744:CDS
AGGGACCATGCTTGTGTTGT+TGG 0.394797 1.2:-83986940 MS.gene99744:CDS
GTCACTAAGAATCTTGTTCC+TGG 0.395090 1.2:-83989299 MS.gene99744:CDS
GGCAGAGGAGGACGTGGAGG+AGG 0.399351 1.2:-83989428 MS.gene99744:CDS
TGATGTTGCTCAGCCTGATC+AGG 0.400610 1.2:-83988436 MS.gene99744:intron
AGAGGTGGCGGTAGAGGCGG+AGG 0.402146 1.2:-83989452 MS.gene99744:CDS
GGAGGGTTCAGAGGAGGAAG+GGG 0.404742 1.2:-83989554 MS.gene99744:CDS
CGTGGAGGAGGCCGTGGTGG+CGG 0.406160 1.2:-83989416 MS.gene99744:CDS
GGTAGAGGCGGAGGAGGCAG+AGG 0.408492 1.2:-83989443 MS.gene99744:CDS
GGTGGCGGTAGAGGCGGAGG+AGG 0.421527 1.2:-83989449 MS.gene99744:CDS
GGGTTCAGAGGAGGAAGGGG+TGG 0.421936 1.2:-83989551 MS.gene99744:CDS
ACACTTTGTTATTTCTATCA+AGG 0.422492 1.2:-83987390 MS.gene99744:intron
TTGGTGGTGTCGACAATATA+TGG 0.424660 1.2:-83988877 MS.gene99744:CDS
GGAGGACGTGGAGGAGGCCG+TGG 0.430468 1.2:-83989422 MS.gene99744:CDS
AAATTAAACCACACCTGATC+AGG 0.438185 1.2:+83988423 None:intergenic
GGAGGACGAGGAGGAGATAG+AGG 0.440185 1.2:-83989491 MS.gene99744:CDS
GGAGGAGGCAGAGGAGGACG+TGG 0.443901 1.2:-83989434 MS.gene99744:CDS
CGTGTTCTGTTACCTCGAAC+AGG 0.448367 1.2:+83989757 None:intergenic
GCCAACCAACATTCTGTACT+TGG 0.454773 1.2:+83988479 None:intergenic
CCATCGAAGTGGGCGTGACT+TGG 0.458744 1.2:-83988562 MS.gene99744:CDS
ATAGAGGAGGTAGAGGAGGT+GGG 0.464408 1.2:-83989526 MS.gene99744:CDS
CGTGGTGGCGGAAGAGGTGG+TGG 0.465952 1.2:-83989404 MS.gene99744:CDS
GCTAGAGGTGGCGGTAGAGG+CGG 0.468529 1.2:-83989455 MS.gene99744:CDS
AAGGCTAGAGGTGGCGGTAG+AGG 0.468910 1.2:-83989458 MS.gene99744:CDS
TCTCATGTGTCTGATATTGT+TGG 0.470006 1.2:-83988719 MS.gene99744:CDS
AGATAGAGGTACCCCTTTCA+AGG 0.470760 1.2:-83989477 MS.gene99744:CDS
AAGTTGGCAGCTGCTGTCCT+TGG 0.472296 1.2:-83988896 MS.gene99744:CDS
GCCGTGGTGGTTCTGGTGGA+GGG 0.473475 1.2:-83989571 MS.gene99744:CDS
GGTAGAGGAGGTGGGAGAGG+TGG 0.474660 1.2:-83989518 MS.gene99744:CDS
CGCCACCTCTAGCCTTGAAA+GGG 0.477576 1.2:+83989465 None:intergenic
GAAAATACCCTCATGTCTAT+GGG 0.477789 1.2:+83989349 None:intergenic
CCAAGTCACGCCCACTTCGA+TGG 0.482871 1.2:+83988562 None:intergenic
AGAGGCGGAGGAGGCAGAGG+AGG 0.484426 1.2:-83989440 MS.gene99744:CDS
GGTGGTGATAGAGGAGGTAG+AGG 0.485873 1.2:-83989533 MS.gene99744:CDS
GGCCGTGGTGGCGGAAGAGG+TGG 0.485933 1.2:-83989407 MS.gene99744:CDS
GGAAGAGGTGGTGGAATGAA+GGG 0.492329 1.2:-83989395 MS.gene99744:CDS
CAAGCATGGTCCCTCTCAAA+TGG 0.492656 1.2:+83986949 None:intergenic
GACCATGCTTGTGTTGTTGG+TGG 0.497588 1.2:-83986937 MS.gene99744:CDS
TGAGAAGAGGGTTACCGTGC+AGG 0.499140 1.2:-83989261 MS.gene99744:intron
ACCAACATTCTGTACTTGGC+GGG 0.501411 1.2:+83988483 None:intergenic
GCTTCATATTATCTCAAAGC+TGG 0.503141 1.2:-83987416 MS.gene99744:CDS
GGTGATAGAGGAGGTAGAGG+AGG 0.504276 1.2:-83989530 MS.gene99744:CDS
CAGAAGCAGCTCCTAGGTAA+AGG 0.506248 1.2:+83988753 None:intergenic
CTCAAAATATAGAGGCTACT+CGG 0.506705 1.2:+83986885 None:intergenic
CCGCCACCTCTAGCCTTGAA+AGG 0.514108 1.2:+83989464 None:intergenic
TTGGCAGCTGCTGTCCTTGG+TGG 0.517332 1.2:-83988893 MS.gene99744:CDS
TTGTTCCAGAAGCAGCTCCT+AGG 0.523899 1.2:+83988747 None:intergenic
TCATTGTAAACAGCTTCTCC+AGG 0.524882 1.2:+83989281 None:intergenic
AACCAACATTCTGTACTTGG+CGG 0.526725 1.2:+83988482 None:intergenic
GGAGGCAGAGGAGGACGTGG+AGG 0.526880 1.2:-83989431 MS.gene99744:CDS
TGGAATGAAGGGAGGAAGCA+AGG 0.532988 1.2:-83989384 MS.gene99744:CDS
ACAATGGCTCCTCCTGTTCG+AGG 0.533054 1.2:-83989769 MS.gene99744:intron
TGGAGGGTTCAGAGGAGGAA+GGG 0.537472 1.2:-83989555 MS.gene99744:CDS
GAACCCTTTCCGTTCTAAGT+TGG 0.539930 1.2:-83988912 MS.gene99744:CDS
GTACAGAATGTTGGTTGGCA+TGG 0.545696 1.2:-83988475 MS.gene99744:CDS
CCTTTCAAGGCTAGAGGTGG+CGG 0.550070 1.2:-83989464 MS.gene99744:CDS
CTGCCAACTTAGAACGGAAA+GGG 0.557631 1.2:+83988909 None:intergenic
AGGTAAAGGACTTTAGCGCC+AGG 0.557851 1.2:+83988767 None:intergenic
CAAGTCACGCCCACTTCGAT+GGG 0.558873 1.2:+83988563 None:intergenic
ACAGGCCGTGGTGGTTCTGG+TGG 0.566444 1.2:-83989575 MS.gene99744:intron
GACTCTTGAACCATTTGAGA+GGG 0.572197 1.2:-83986959 MS.gene99744:CDS
GTGGAGGGTTCAGAGGAGGA+AGG 0.574068 1.2:-83989556 MS.gene99744:CDS
GGTTCTGGTGGAGGGTTCAG+AGG 0.575244 1.2:-83989563 MS.gene99744:CDS
AGAGGTGGTGGAATGAAGGG+AGG 0.576093 1.2:-83989392 MS.gene99744:CDS
GGACGTGGAGGAGGCCGTGG+TGG 0.585474 1.2:-83989419 MS.gene99744:CDS
CAGCAGCTGCCAACTTAGAA+CGG 0.593341 1.2:+83988903 None:intergenic
TCTGGTGGAGGGTTCAGAGG+AGG 0.598909 1.2:-83989560 MS.gene99744:CDS
GGAAGGGGTGGTGATAGAGG+AGG 0.599693 1.2:-83989539 MS.gene99744:CDS
AAGCTGTTTACAATGAGAAG+AGG 0.601232 1.2:-83989274 MS.gene99744:CDS
AGCTGTTTACAATGAGAAGA+GGG 0.603845 1.2:-83989273 MS.gene99744:CDS
GTTGAACCCCATAGACATGA+GGG 0.606951 1.2:-83989356 MS.gene99744:CDS
TGACTCTTGAACCATTTGAG+AGG 0.609035 1.2:-83986960 MS.gene99744:CDS
GCCAAGTACAGAATGTTGGT+TGG 0.614552 1.2:-83988480 MS.gene99744:CDS
TCCCGCCAAGTACAGAATGT+TGG 0.621000 1.2:-83988484 MS.gene99744:CDS
TGAATTTGTACAGGCCGTGG+TGG 0.621278 1.2:-83989584 MS.gene99744:intron
TGTTGAACCCCATAGACATG+AGG 0.622170 1.2:-83989357 MS.gene99744:CDS
TAGAATTCTCCCATCGAAGT+GGG 0.624110 1.2:-83988572 MS.gene99744:CDS
TCATATTATCTCAAAGCTGG+AGG 0.625407 1.2:-83987413 MS.gene99744:CDS
ACCCCTTTCAAGGCTAGAGG+TGG 0.626063 1.2:-83989467 MS.gene99744:CDS
GCTGCCAACTTAGAACGGAA+AGG 0.630241 1.2:+83988908 None:intergenic
CACCACCTCTTCCGCCACCA+CGG 0.647973 1.2:+83989405 None:intergenic
AGCCACCAACAACACAAGCA+TGG 0.648785 1.2:+83986935 None:intergenic
GGTACCCCTTTCAAGGCTAG+AGG 0.653441 1.2:-83989470 MS.gene99744:CDS
AAAATACCCTCATGTCTATG+GGG 0.663716 1.2:+83989350 None:intergenic
GTAGAATTCTCCCATCGAAG+TGG 0.663836 1.2:-83988573 MS.gene99744:CDS
GGCGCTAAAGTCCTTTACCT+AGG 0.679116 1.2:-83988764 MS.gene99744:CDS
ACCCTCCACCAGAACCACCA+CGG 0.696616 1.2:+83989570 None:intergenic
GCCACCTCTAGCCTTGAAAG+GGG 0.697135 1.2:+83989466 None:intergenic
TGAGAAAGGAAATACCTGCA+CGG 0.697697 1.2:+83989247 None:intergenic
GTTCTGTTACCTCGAACAGG+AGG 0.699595 1.2:+83989760 None:intergenic
ATATATTGTCGACACCACCA+AGG 0.737399 1.2:+83988879 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAAATTTTAATTTTTTTTTT+TGG - chr1.2:83989558-83989577 MS.gene99744:CDS 0.0%
!! GAAAATCATTAAAATAAATT+AGG + chr1.2:83988890-83988909 None:intergenic 10.0%
!! TGAAAATTTATAAACAAAAT+TGG + chr1.2:83988637-83988656 None:intergenic 10.0%
!!! TTTATTTGTTAAAATCAAAT+GGG - chr1.2:83986982-83987001 MS.gene99744:intron 10.0%
!!! TTTTTAATTTGTTAACTTTT+TGG - chr1.2:83988913-83988932 MS.gene99744:CDS 10.0%
!! ATATGATGTTTATTAAATAG+TGG - chr1.2:83988978-83988997 MS.gene99744:intron 15.0%
!! TAATGCATAACAAAAAAATA+CGG + chr1.2:83989380-83989399 None:intergenic 15.0%
!! TGTCAATATAATGTATTTAA+AGG + chr1.2:83987853-83987872 None:intergenic 15.0%
!!! CAATTTTATTTTGTATTTGT+AGG - chr1.2:83989590-83989609 MS.gene99744:intron 15.0%
!!! CATTTGATTTTAACAAATAA+AGG + chr1.2:83986983-83987002 None:intergenic 15.0%
!!! CTTTATTTGTTAAAATCAAA+TGG - chr1.2:83986981-83987000 MS.gene99744:intron 15.0%
!!! TTATATTGACATCTTAATTT+TGG - chr1.2:83987862-83987881 MS.gene99744:intron 15.0%
!! ATCATCAATAAACTCTAATT+CGG + chr1.2:83986949-83986968 None:intergenic 20.0%
!! ATGTTATTGTCTAGTAAAAT+AGG + chr1.2:83987548-83987567 None:intergenic 20.0%
!! CATGTTATTCTATTTAATAG+TGG - chr1.2:83987666-83987685 MS.gene99744:intron 20.0%
!! CGAAAATTTATAAACAAAAC+AGG + chr1.2:83988611-83988630 None:intergenic 20.0%
!!! GTGTAAAATGTAAAATGTTT+TGG - chr1.2:83988442-83988461 MS.gene99744:CDS 20.0%
!!! TGTTTTATAATGTTAAGAAC+AGG - chr1.2:83988796-83988815 MS.gene99744:intron 20.0%
!!! TTTTAAAGCTTACTTTCTAT+AGG - chr1.2:83989338-83989357 MS.gene99744:CDS 20.0%
! AACACAAAACATACATATGT+TGG + chr1.2:83988019-83988038 None:intergenic 25.0%
! ACTTCAATAACAATAAACCA+TGG - chr1.2:83989399-83989418 MS.gene99744:CDS 25.0%
! ATAATGTTAAGAACAGGATT+GGG - chr1.2:83988802-83988821 MS.gene99744:intron 25.0%
! ATCTATCCAATGAAGTTATA+AGG + chr1.2:83989461-83989480 None:intergenic 25.0%
! CTTCAATAACAATAAACCAT+GGG - chr1.2:83989400-83989419 MS.gene99744:CDS 25.0%
! TAAAATAAATTAGGCTCAAC+TGG + chr1.2:83988881-83988900 None:intergenic 25.0%
! TATAATGTTAAGAACAGGAT+TGG - chr1.2:83988801-83988820 MS.gene99744:intron 25.0%
! TATACTATGTTGATATTTGC+AGG - chr1.2:83989207-83989226 MS.gene99744:intron 25.0%
! TGATGTTTATTAAATAGTGG+TGG - chr1.2:83988981-83989000 MS.gene99744:intron 25.0%
!! ATATGTATGTTTTGTGTTTG+AGG - chr1.2:83988021-83988040 MS.gene99744:intron 25.0%
!! ATTCATTCACCATTTTACAA+AGG + chr1.2:83989498-83989517 None:intergenic 25.0%
!! TTTTGACCTTATAACTTCAT+TGG - chr1.2:83989452-83989471 MS.gene99744:CDS 25.0%
!!! ACACTTTGTTATTTCTATCA+AGG - chr1.2:83989276-83989295 MS.gene99744:CDS 25.0%
!!! AGCTTTAAAAAACAAGAAAC+TGG + chr1.2:83989328-83989347 None:intergenic 25.0%
AAATATGTGCATCAGAATCT+CGG + chr1.2:83988537-83988556 None:intergenic 30.0%
AACTGTGGAATCAATACAAT+TGG + chr1.2:83989616-83989635 None:intergenic 30.0%
AATAAACTCTAATTCGGACA+AGG + chr1.2:83986943-83986962 None:intergenic 30.0%
AATGTTAACCAAATAGCAGT+GGG - chr1.2:83989149-83989168 MS.gene99744:intron 30.0%
AGATTGCAAATCAACAATCA+AGG + chr1.2:83989069-83989088 None:intergenic 30.0%
CAAGGATATAATGCAAGATA+CGG + chr1.2:83989051-83989070 None:intergenic 30.0%
CTAAGACAGAGTATAGAATT+TGG - chr1.2:83987732-83987751 MS.gene99744:intron 30.0%
CTGTAAGTATTAGTTCAACT+TGG - chr1.2:83987972-83987991 MS.gene99744:intron 30.0%
CTTACTTTCTATAGGAATCT+TGG - chr1.2:83989346-83989365 MS.gene99744:CDS 30.0%
TAATGTTAACCAAATAGCAG+TGG - chr1.2:83989148-83989167 MS.gene99744:intron 30.0%
TAATGTTAAGAACAGGATTG+GGG - chr1.2:83988803-83988822 MS.gene99744:intron 30.0%
TATAACATTAGTACGCTTCT+TGG + chr1.2:83988139-83988158 None:intergenic 30.0%
TGCATTAGTAGAAAAGTGAA+TGG + chr1.2:83988326-83988345 None:intergenic 30.0%
TGTCTAGCATCTTCAATAAT+TGG + chr1.2:83988162-83988181 None:intergenic 30.0%
! ACCATTTTACAAAGGTTTGA+AGG + chr1.2:83989490-83989509 None:intergenic 30.0%
! ACTGATTTAATGTGTCTTGA+TGG - chr1.2:83988385-83988404 MS.gene99744:intron 30.0%
! AGATTCTGATGCACATATTT+TGG - chr1.2:83988537-83988556 MS.gene99744:CDS 30.0%
! CAAGTTGAACTAATACTTAC+AGG + chr1.2:83987974-83987993 None:intergenic 30.0%
! CCATTTTACAAAGGTTTGAA+GGG + chr1.2:83989489-83989508 None:intergenic 30.0%
! TATTTGCAGGCAAGAATTTT+AGG - chr1.2:83989220-83989239 MS.gene99744:intron 30.0%
!! TTGATTGTTGATTTGCAATC+TGG - chr1.2:83989068-83989087 MS.gene99744:intron 30.0%
!! TTGGTTTTTATCTAGAAACC+TGG - chr1.2:83987881-83987900 MS.gene99744:intron 30.0%
!!! TGTTTTGGTTACTACAACAT+CGG - chr1.2:83988457-83988476 MS.gene99744:CDS 30.0%
AAAATACCCTCATGTCTATG+GGG + chr1.2:83987319-83987338 None:intergenic 35.0%
AAATTAAACCACACCTGATC+AGG + chr1.2:83988246-83988265 None:intergenic 35.0%
AAGCTGTTTACAATGAGAAG+AGG - chr1.2:83987392-83987411 MS.gene99744:CDS 35.0%
AATATGTGCATCAGAATCTC+GGG + chr1.2:83988536-83988555 None:intergenic 35.0%
AATGTTAAGAACAGGATTGG+GGG - chr1.2:83988804-83988823 MS.gene99744:intron 35.0%
AGCTGATCAATTTAAGCCAA+TGG - chr1.2:83989677-83989696 MS.gene99744:intron 35.0%
AGCTGTTTACAATGAGAAGA+GGG - chr1.2:83987393-83987412 MS.gene99744:CDS 35.0%
ATCATCCAAGAAGTCTTGAA+GGG + chr1.2:83988508-83988527 None:intergenic 35.0%
ATTCAAAGCACAAACATCCA+AGG + chr1.2:83987446-83987465 None:intergenic 35.0%
ATTGATGTGCATCAGAATCT+CGG + chr1.2:83988749-83988768 None:intergenic 35.0%
CAATAAACCATGGGAAAATG+AGG - chr1.2:83989409-83989428 MS.gene99744:CDS 35.0%
CATGTTCTGTTTATGTAGAC+TGG - chr1.2:83988057-83988076 MS.gene99744:intron 35.0%
CCCTTCAAACCTTTGTAAAA+TGG - chr1.2:83989486-83989505 MS.gene99744:CDS 35.0%
GAAAATACCCTCATGTCTAT+GGG + chr1.2:83987320-83987339 None:intergenic 35.0%
GCTTCATATTATCTCAAAGC+TGG - chr1.2:83989250-83989269 MS.gene99744:intron 35.0%
TAGGCTCAACTGGAAAAAAA+GGG + chr1.2:83988871-83988890 None:intergenic 35.0%
TCATATTATCTCAAAGCTGG+AGG - chr1.2:83989253-83989272 MS.gene99744:intron 35.0%
TCTCATGTGTCTGATATTGT+TGG - chr1.2:83987947-83987966 MS.gene99744:intron 35.0%
TGAAAATACCCTCATGTCTA+TGG + chr1.2:83987321-83987340 None:intergenic 35.0%
TGGGAAAATGAGGTTAGTAT+TGG - chr1.2:83989419-83989438 MS.gene99744:CDS 35.0%
TTAGGCTCAACTGGAAAAAA+AGG + chr1.2:83988872-83988891 None:intergenic 35.0%
TTGTTGCGTTGAATTTGTAC+AGG - chr1.2:83987073-83987092 MS.gene99744:intron 35.0%
! ATACTAACCTCATTTTCCCA+TGG + chr1.2:83989419-83989438 None:intergenic 35.0%
! GACATATTTTCTGCAGAATG+AGG - chr1.2:83987703-83987722 MS.gene99744:intron 35.0%
! TATTTTCTGCAGAATGAGGA+TGG - chr1.2:83987707-83987726 MS.gene99744:intron 35.0%
!! AAAATGACTTTGAGACTGCA+AGG + chr1.2:83989016-83989035 None:intergenic 35.0%
!! TAATGACTTGTTTAGGCACA+TGG - chr1.2:83988830-83988849 MS.gene99744:intron 35.0%
!! TGCAGTCTCAAAGTCATTTT+TGG - chr1.2:83989016-83989035 MS.gene99744:intron 35.0%
!!! GAGGGTATTTTCATTGCTAA+GGG - chr1.2:83987328-83987347 MS.gene99744:intron 35.0%
!!! TCGGCATCTTTTTTCTTCTT+GGG + chr1.2:83989765-83989784 None:intergenic 35.0%
!!! TGAGGGTATTTTCATTGCTA+AGG - chr1.2:83987327-83987346 MS.gene99744:intron 35.0%
AACCAACATTCTGTACTTGG+CGG + chr1.2:83988187-83988206 None:intergenic 40.0%
AACTCTAATTCGGACAAGGA+TGG + chr1.2:83986939-83986958 None:intergenic 40.0%
ACACTGACACGTAGAATACA+TGG + chr1.2:83989183-83989202 None:intergenic 40.0%
AGTGCATGTGATGAGTTACT+CGG - chr1.2:83988690-83988709 MS.gene99744:intron 40.0%
ATATATTGTCGACACCACCA+AGG + chr1.2:83987790-83987809 None:intergenic 40.0%
CAAACATCCAAGGATGAGAA+AGG + chr1.2:83987436-83987455 None:intergenic 40.0%
CATCATCCAAGAAGTCTTGA+AGG + chr1.2:83988509-83988528 None:intergenic 40.0%
GACTCTTGAACCATTTGAGA+GGG - chr1.2:83989707-83989726 MS.gene99744:intron 40.0%
GGTATTTCCTTTCTCATCCT+TGG - chr1.2:83987426-83987445 MS.gene99744:CDS 40.0%
GTCACTAAGAATCTTGTTCC+TGG - chr1.2:83987367-83987386 MS.gene99744:intron 40.0%
TAGAATTCTCCCATCGAAGT+GGG - chr1.2:83988094-83988113 MS.gene99744:intron 40.0%
TCATCCAAGAAGTCTTGAAG+GGG + chr1.2:83988507-83988526 None:intergenic 40.0%
TCATTGTAAACAGCTTCTCC+AGG + chr1.2:83987388-83987407 None:intergenic 40.0%
TGACTCTTGAACCATTTGAG+AGG - chr1.2:83989706-83989725 MS.gene99744:intron 40.0%
TGAGAAAGGAAATACCTGCA+CGG + chr1.2:83987422-83987441 None:intergenic 40.0%
TGTCAATGTTGATGCTTGCT+TGG - chr1.2:83989521-83989540 MS.gene99744:CDS 40.0%
TTGATGTGCATCAGAATCTC+GGG + chr1.2:83988748-83988767 None:intergenic 40.0%
!! TTGGTGGTGTCGACAATATA+TGG - chr1.2:83987789-83987808 MS.gene99744:intron 40.0%
!!! CTCGGCATCTTTTTTCTTCT+TGG + chr1.2:83989766-83989785 None:intergenic 40.0%
ACCAACATTCTGTACTTGGC+GGG + chr1.2:83988186-83988205 None:intergenic 45.0%
AGATTCGTCCCACTGCTATT+TGG + chr1.2:83989160-83989179 None:intergenic 45.0%
CTGCCAACTTAGAACGGAAA+GGG + chr1.2:83987760-83987779 None:intergenic 45.0%
GATTCCCCTTCAAGACTTCT+TGG - chr1.2:83988500-83988519 MS.gene99744:CDS 45.0%
GCCAACCAACATTCTGTACT+TGG + chr1.2:83988190-83988209 None:intergenic 45.0%
GCCAAGTACAGAATGTTGGT+TGG - chr1.2:83988186-83988205 MS.gene99744:intron 45.0%
GGTGCATGTGATGAGTTACT+CGG - chr1.2:83988478-83988497 MS.gene99744:CDS 45.0%
GTAGAATTCTCCCATCGAAG+TGG - chr1.2:83988093-83988112 MS.gene99744:intron 45.0%
GTTGAACCCCATAGACATGA+GGG - chr1.2:83987310-83987329 MS.gene99744:intron 45.0%
TCAAGAGTCACTTGCTCCAT+TGG + chr1.2:83989696-83989715 None:intergenic 45.0%
TGTTGAACCCCATAGACATG+AGG - chr1.2:83987309-83987328 MS.gene99744:intron 45.0%
! GAACCCTTTCCGTTCTAAGT+TGG - chr1.2:83987754-83987773 MS.gene99744:intron 45.0%
! GTACAGAATGTTGGTTGGCA+TGG - chr1.2:83988191-83988210 MS.gene99744:intron 45.0%
!! AGATAGAGGTACCCCTTTCA+AGG - chr1.2:83987189-83987208 MS.gene99744:intron 45.0%
!! GGGGCAGTAATGACTTGTTT+AGG - chr1.2:83988823-83988842 MS.gene99744:intron 45.0%
AGCCACCAACAACACAAGCA+TGG + chr1.2:83989734-83989753 None:intergenic 50.0%
AGGGACCATGCTTGTGTTGT+TGG - chr1.2:83989726-83989745 MS.gene99744:intron 50.0%
AGGTAAAGGACTTTAGCGCC+AGG + chr1.2:83987902-83987921 None:intergenic 50.0%
ATAGAGGAGGTAGAGGAGGT+GGG - chr1.2:83987140-83987159 MS.gene99744:intron 50.0%
CAAGCATGGTCCCTCTCAAA+TGG + chr1.2:83989720-83989739 None:intergenic 50.0%
CAGAAGCAGCTCCTAGGTAA+AGG + chr1.2:83987916-83987935 None:intergenic 50.0%
CAGCAGCTGCCAACTTAGAA+CGG + chr1.2:83987766-83987785 None:intergenic 50.0%
CGTGTTCTGTTACCTCGAAC+AGG + chr1.2:83986912-83986931 None:intergenic 50.0%
CGTTGAATTTGTACAGGCCG+TGG - chr1.2:83987079-83987098 MS.gene99744:intron 50.0%
CTTTACCTAGGAGCTGCTTC+TGG - chr1.2:83987914-83987933 MS.gene99744:intron 50.0%
GCTGCCAACTTAGAACGGAA+AGG + chr1.2:83987761-83987780 None:intergenic 50.0%
GGCGCTAAAGTCCTTTACCT+AGG - chr1.2:83987902-83987921 MS.gene99744:intron 50.0%
GTTCTGTTACCTCGAACAGG+AGG + chr1.2:83986909-83986928 None:intergenic 50.0%
TCCCGCCAAGTACAGAATGT+TGG - chr1.2:83988182-83988201 MS.gene99744:intron 50.0%
TGAATTTGTACAGGCCGTGG+TGG - chr1.2:83987082-83987101 MS.gene99744:intron 50.0%
TGATGTTGCTCAGCCTGATC+AGG - chr1.2:83988230-83988249 MS.gene99744:intron 50.0%
TGATTCCACAGTTCCAGCTG+AGG - chr1.2:83989623-83989642 MS.gene99744:intron 50.0%
TGGAATGAAGGGAGGAAGCA+AGG - chr1.2:83987282-83987301 MS.gene99744:intron 50.0%
TTGTTCCAGAAGCAGCTCCT+AGG + chr1.2:83987922-83987941 None:intergenic 50.0%
! GACCATGCTTGTGTTGTTGG+TGG - chr1.2:83989729-83989748 MS.gene99744:intron 50.0%
!! GGAAGAGGTGGTGGAATGAA+GGG - chr1.2:83987271-83987290 MS.gene99744:intron 50.0%
!! TGGGCGTGACTTGGTCAATA+TGG - chr1.2:83988113-83988132 MS.gene99744:intron 50.0%
AAGTTGGCAGCTGCTGTCCT+TGG - chr1.2:83987770-83987789 MS.gene99744:intron 55.0%
ACCCCTTTCAAGGCTAGAGG+TGG - chr1.2:83987199-83987218 MS.gene99744:intron 55.0%
CAAGTCACGCCCACTTCGAT+GGG + chr1.2:83988106-83988125 None:intergenic 55.0%
CCTTTCAAGGCTAGAGGTGG+CGG - chr1.2:83987202-83987221 MS.gene99744:intron 55.0%
GATAGAGGAGGTAGAGGAGG+TGG - chr1.2:83987139-83987158 MS.gene99744:intron 55.0%
GGTACCCCTTTCAAGGCTAG+AGG - chr1.2:83987196-83987215 MS.gene99744:intron 55.0%
GGTGATAGAGGAGGTAGAGG+AGG - chr1.2:83987136-83987155 MS.gene99744:intron 55.0%
GGTGGTGATAGAGGAGGTAG+AGG - chr1.2:83987133-83987152 MS.gene99744:intron 55.0%
TACTGCCTCAGCTGGAACTG+TGG + chr1.2:83989631-83989650 None:intergenic 55.0%
TGAGAAGAGGGTTACCGTGC+AGG - chr1.2:83987405-83987424 MS.gene99744:CDS 55.0%
TGAGGCAGTATTCAGCGCTG+AGG - chr1.2:83989641-83989660 MS.gene99744:intron 55.0%
TGGAGGGTTCAGAGGAGGAA+GGG - chr1.2:83987111-83987130 MS.gene99744:intron 55.0%
TTGCTCAGCCTGATCAGGTG+TGG - chr1.2:83988235-83988254 MS.gene99744:intron 55.0%
!! AGAGGTGGTGGAATGAAGGG+AGG - chr1.2:83987274-83987293 MS.gene99744:intron 55.0%
!! CGCCACCTCTAGCCTTGAAA+GGG + chr1.2:83987204-83987223 None:intergenic 55.0%
!! CGGAAGAGGTGGTGGAATGA+AGG - chr1.2:83987270-83987289 MS.gene99744:intron 55.0%
!! GCCACCTCTAGCCTTGAAAG+GGG + chr1.2:83987203-83987222 None:intergenic 55.0%
!!! AGAGGTGGTTTTGGAGGACG+AGG - chr1.2:83987163-83987182 MS.gene99744:intron 55.0%
ACCCTCCACCAGAACCACCA+CGG + chr1.2:83987099-83987118 None:intergenic 60.0%
CCAAGTCACGCCCACTTCGA+TGG + chr1.2:83988107-83988126 None:intergenic 60.0%
CCATCGAAGTGGGCGTGACT+TGG - chr1.2:83988104-83988123 MS.gene99744:intron 60.0%
GCGCTGAATACTGCCTCAGC+TGG + chr1.2:83989639-83989658 None:intergenic 60.0%
GGAAGGGGTGGTGATAGAGG+AGG - chr1.2:83987127-83987146 MS.gene99744:intron 60.0%
GGAGGAAGGGGTGGTGATAG+AGG - chr1.2:83987124-83987143 MS.gene99744:intron 60.0%
GGAGGACGAGGAGGAGATAG+AGG - chr1.2:83987175-83987194 MS.gene99744:intron 60.0%
GGAGGGTTCAGAGGAGGAAG+GGG - chr1.2:83987112-83987131 MS.gene99744:intron 60.0%
GTGGAGGGTTCAGAGGAGGA+AGG - chr1.2:83987110-83987129 MS.gene99744:intron 60.0%
TCTGGTGGAGGGTTCAGAGG+AGG - chr1.2:83987106-83987125 MS.gene99744:intron 60.0%
TGTACAGGCCGTGGTGGTTC+TGG - chr1.2:83987088-83987107 MS.gene99744:intron 60.0%
TTGGCAGCTGCTGTCCTTGG+TGG - chr1.2:83987773-83987792 MS.gene99744:intron 60.0%
! AAGGCTAGAGGTGGCGGTAG+AGG - chr1.2:83987208-83987227 MS.gene99744:intron 60.0%
! GGAGGTGGGAGAGGTGGTTT+TGG - chr1.2:83987154-83987173 MS.gene99744:intron 60.0%
! GGTTCTGGTGGAGGGTTCAG+AGG - chr1.2:83987103-83987122 MS.gene99744:intron 60.0%
!! CCGCCACCTCTAGCCTTGAA+AGG + chr1.2:83987205-83987224 None:intergenic 60.0%
!!! GGTGGGAGAGGTGGTTTTGG+AGG - chr1.2:83987157-83987176 MS.gene99744:intron 60.0%
!!! GGTGGTTTTGGAGGACGAGG+AGG - chr1.2:83987166-83987185 MS.gene99744:intron 60.0%
CACCACCTCTTCCGCCACCA+CGG + chr1.2:83987264-83987283 None:intergenic 65.0%
GGAGGTAGAGGAGGTGGGAG+AGG - chr1.2:83987145-83987164 MS.gene99744:intron 65.0%
GGGTTCAGAGGAGGAAGGGG+TGG - chr1.2:83987115-83987134 MS.gene99744:intron 65.0%
GGTAGAGGAGGTGGGAGAGG+TGG - chr1.2:83987148-83987167 MS.gene99744:intron 65.0%
! GCTAGAGGTGGCGGTAGAGG+CGG - chr1.2:83987211-83987230 MS.gene99744:intron 65.0%
!! ACAGGCCGTGGTGGTTCTGG+TGG - chr1.2:83987091-83987110 MS.gene99744:intron 65.0%
!! GCCGTGGTGGTTCTGGTGGA+GGG - chr1.2:83987095-83987114 MS.gene99744:intron 65.0%
AGAGGCGGAGGAGGCAGAGG+AGG - chr1.2:83987226-83987245 MS.gene99744:intron 70.0%
GGAGGAGGCAGAGGAGGACG+TGG - chr1.2:83987232-83987251 MS.gene99744:intron 70.0%
GGAGGCAGAGGAGGACGTGG+AGG - chr1.2:83987235-83987254 MS.gene99744:intron 70.0%
GGCAGAGGAGGACGTGGAGG+AGG - chr1.2:83987238-83987257 MS.gene99744:intron 70.0%
GGTAGAGGCGGAGGAGGCAG+AGG - chr1.2:83987223-83987242 MS.gene99744:intron 70.0%
! AGAGGTGGCGGTAGAGGCGG+AGG - chr1.2:83987214-83987233 MS.gene99744:intron 70.0%
!! CGTGGTGGCGGAAGAGGTGG+TGG - chr1.2:83987262-83987281 MS.gene99744:intron 70.0%
!! GGCCGTGGTGGTTCTGGTGG+AGG - chr1.2:83987094-83987113 MS.gene99744:intron 70.0%
CGTGGAGGAGGCCGTGGTGG+CGG - chr1.2:83987250-83987269 MS.gene99744:intron 75.0%
GGACGTGGAGGAGGCCGTGG+TGG - chr1.2:83987247-83987266 MS.gene99744:intron 75.0%
GGAGGACGTGGAGGAGGCCG+TGG - chr1.2:83987244-83987263 MS.gene99744:intron 75.0%
! GGTGGCGGTAGAGGCGGAGG+AGG - chr1.2:83987217-83987236 MS.gene99744:intron 75.0%
!! GGAGGCCGTGGTGGCGGAAG+AGG - chr1.2:83987256-83987275 MS.gene99744:intron 75.0%
!! GGCCGTGGTGGCGGAAGAGG+TGG - chr1.2:83987259-83987278 MS.gene99744:intron 75.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 83986900 83989788 83986900 ID=MS.gene99744
chr1.2 mRNA 83986900 83989788 83986900 ID=MS.gene99744.t1;Parent=MS.gene99744
chr1.2 exon 83989770 83989788 83989770 ID=MS.gene99744.t1.exon1;Parent=MS.gene99744.t1
chr1.2 CDS 83989770 83989788 83989770 ID=cds.MS.gene99744.t1;Parent=MS.gene99744.t1
chr1.2 exon 83989262 83989593 83989262 ID=MS.gene99744.t1.exon2;Parent=MS.gene99744.t1
chr1.2 CDS 83989262 83989593 83989262 ID=cds.MS.gene99744.t1;Parent=MS.gene99744.t1
chr1.2 exon 83988874 83988969 83988874 ID=MS.gene99744.t1.exon3;Parent=MS.gene99744.t1
chr1.2 CDS 83988874 83988969 83988874 ID=cds.MS.gene99744.t1;Parent=MS.gene99744.t1
chr1.2 exon 83988715 83988792 83988715 ID=MS.gene99744.t1.exon4;Parent=MS.gene99744.t1
chr1.2 CDS 83988715 83988792 83988715 ID=cds.MS.gene99744.t1;Parent=MS.gene99744.t1
chr1.2 exon 83988437 83988613 83988437 ID=MS.gene99744.t1.exon5;Parent=MS.gene99744.t1
chr1.2 CDS 83988437 83988613 83988437 ID=cds.MS.gene99744.t1;Parent=MS.gene99744.t1
chr1.2 exon 83987391 83987459 83987391 ID=MS.gene99744.t1.exon6;Parent=MS.gene99744.t1
chr1.2 CDS 83987391 83987459 83987391 ID=cds.MS.gene99744.t1;Parent=MS.gene99744.t1
chr1.2 exon 83986900 83986995 83986900 ID=MS.gene99744.t1.exon7;Parent=MS.gene99744.t1
chr1.2 CDS 83986900 83986995 83986900 ID=cds.MS.gene99744.t1;Parent=MS.gene99744.t1
Gene Sequence

>MS.gene99744

ATGGCTCCTCCTGTTCGAGGTAACAGAACACGTTAACCATCCTTGTCCGAATTAGAGTTTATTGATGATGCTTTTGTTTACCTTTATTTGTTAAAATCAAATGGGTTTACGCTAATTTGCTCAAAAGAATGATTTTGCTTGTTTTATGAACTATATAGTGATTTTTGTTGATATTGTTGCGTTGAATTTGTACAGGCCGTGGTGGTTCTGGTGGAGGGTTCAGAGGAGGAAGGGGTGGTGATAGAGGAGGTAGAGGAGGTGGGAGAGGTGGTTTTGGAGGACGAGGAGGAGATAGAGGTACCCCTTTCAAGGCTAGAGGTGGCGGTAGAGGCGGAGGAGGCAGAGGAGGACGTGGAGGAGGCCGTGGTGGCGGAAGAGGTGGTGGAATGAAGGGAGGAAGCAAGGTTATTGTTGAACCCCATAGACATGAGGGTATTTTCATTGCTAAGGGTAAAGAAGATGCTCTTGTCACTAAGAATCTTGTTCCTGGAGAAGCTGTTTACAATGAGAAGAGGGTTACCGTGCAGGTATTTCCTTTCTCATCCTTGGATGTTTGTGCTTTGAATTTATTTAGCTTTTTTTAAATTAGTAGCTGTCGATTGAGTTTAACTTACATGTTTATGTGTTGCAATCAATCATTTTGTTCCTATTTTACTAGACAATAACATCTCTTTTACTTGATTTTATCACATAAATGTGCTTACAAATCTTGCAAAATATTTTTCTGAATGTGATATTTTTTTTCAAGATTTCAGTGTGTATCAGTCATGTTATTCTATTTAATAGTGGCATGACAATTTTGTGACATATTTTCTGCAGAATGAGGATGGTACTAAGACAGAGTATAGAATTTGGAACCCTTTCCGTTCTAAGTTGGCAGCTGCTGTCCTTGGTGGTGTCGACAATATATGGATTGTAAGTTGCTTCATTTTTCTGTAACATTACACTGTCCTTTAAATACATTATATTGACATCTTAATTTTGGTTTTTATCTAGAAACCTGGCGCTAAAGTCCTTTACCTAGGAGCTGCTTCTGGAACAACTGTGTCTCATGTGTCTGATATTGTTGGTCCTGTAAGTATTAGTTCAACTTGGTTGATTTTTTTTTTTCTTCTTCCAACATATGTATGTTTTGTGTTTGAGGCTGATTAGAATATCATGTTCTGTTTATGTAGACTGGAGTTGTGTATGCAGTAGAATTCTCCCATCGAAGTGGGCGTGACTTGGTCAATATGGCCAAGAAGCGTACTAATGTTATACCAATTATTGAAGATGCTAGACATCCCGCCAAGTACAGAATGTTGGTTGGCATGGTAGATGTGATATTTTCTGATGTTGCTCAGCCTGATCAGGTGTGGTTTAATTTTTCACTCTTTTTTATGTTAAGAGTACTAGCTATATATTTTTAACTCTCATGATAGTACCATTCACTTTTCTACTAATGCATAACATTAATCTCTACTATGTCATTGTAGTAATCTATTTACTGATTTAATGTGTCTTGATGGAATGCATAATTTGTTTATTATTGTTTATTTCAAAGTGTAAAATGTAAAATGTTTTGGTTACTACAACATCGGTGCATGTGATGAGTTACTCGGATTCCCCTTCAAGACTTCTTGGATGATGTAGACCCGAGATTCTGATGCACATATTTTGGTCTTTTTCTTTATATTGTTTTACTAAAAACTAATACTGTATTTTAAATCCTGTTTTGTTTATAAATTTTCGTTTCCAATTTTGTTTATAAATTTTCATTTCAAAGTGTAAAATGTTTTGATCACTACATCAGTGCATGTGATGAGTTACTCGGATTCCCCTTCAAGACTTCTTGGATGATGTAGACCCGAGATTCTGATGCACATCAATTTTTTTTTTCTCTCTATTTATCTATATTGTTTTATAATGTTAAGAACAGGATTGGGGGCAGTAATGACTTGTTTAGGCACATGGTTTTTGTTTCAGATTCCCTTTTTTTCCAGTTGAGCCTAATTTATTTTAATGATTTTCAGATTTTTAATTTGTTAACTTTTTGGTGTTTGTTTTCTATGAATGAATTTGATCGAAATGTGTTGTATATATGATGTTTATTAAATAGTGGTGGCTTGAAACACCTTGCAGTCTCAAAGTCATTTTTGGTTTGCGTTTCCGTATCTTGCATTATATCCTTGATTGTTGATTTGCAATCTGGATTTTTATTTGTTACTTTTGATGTCTGTTTGTTTTTAATAAATATAAGTGTAAGTGTTAATGTTAACCAAATAGCAGTGGGACGAATCTCCATGTATTCTACGTGTCAGTGTTGATTATACTATGTTGATATTTGCAGGCAAGAATTTTAGGTCTGAATGCTTCATATTATCTCAAAGCTGGAGGACACTTTGTTATTTCTATCAAGGTTTGTTCAGAAATCTATTGTATTAAACCAGTTTCTTGTTTTTTAAAGCTTACTTTCTATAGGAATCTTGGAAAACAATCCGTATTTTTTTGTTATGCATTACTTCAATAACAATAAACCATGGGAAAATGAGGTTAGTATTGGCGTGTGCTCATTTTGACCTTATAACTTCATTGGATAGATAAAATCCCTTCAAACCTTTGTAAAATGGTGAATGAATTTATGTCAATGTTGATGCTTGCTTGGCTTTGAATTAAGTTTAAATTTTAATTTTTTTTTTTGGTTTCTAAATCAATTTTATTTTGTATTTGTAGGCCAATTGTATTGATTCCACAGTTCCAGCTGAGGCAGTATTCAGCGCTGAGGTGAACAAGTTGAAAGCTGATCAATTTAAGCCAATGGAGCAAGTGACTCTTGAACCATTTGAGAGGGACCATGCTTGTGTTGTTGGTGGCTATAGAGTGCCCAAGAAGAAAAAAGATGCCGAGTAG
Protein sequence

>MS.gene99744.t1

MAPPVRGRGGSGGGFRGGRGGDRGGRGGGRGGFGGRGGDRGTPFKARGGGRGGGGRGGRGGGRGGGRGGGMKGGSKVIVEPHRHEGIFIAKGKEDALVTKNLVPGEAVYNEKRVTVQNEDGTKTEYRIWNPFRSKLAAAVLGGVDNIWIKPGAKVLYLGAASGTTVSHVSDIVGPTGVVYAVEFSHRSGRDLVNMAKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILGLNASYYLKAGGHFVISIKPMEQVTLEPFERDHACVVGGYRVPKKKKDAE