AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene08870


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene08870.t1 MTR_4g059400 99.398 166 1 0 1 166 1 166 1.89e-116 327
MS.gene08870.t1 MTR_1g064500 99.390 164 1 0 1 164 1 164 2.24e-114 321
MS.gene08870.t1 MTR_2g094300 83.537 164 27 0 1 164 1 164 1.50e-97 279
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene08870.t1 AT5G60670 90.361 166 16 0 1 166 1 166 9.53e-108 305
MS.gene08870.t1 AT2G37190 89.759 166 17 0 1 166 1 166 1.20e-107 304
MS.gene08870.t1 AT3G53430 89.157 166 18 0 1 166 1 166 2.70e-107 303

Find 0 sgRNAs with CRISPR-Local

Find 64 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
ACTAAGAACATCAAGCATAA+TGG + 32375:9379-9398 MS.gene08870:CDS 30.0%
CTAAGAACATCAAGCATAAT+GGG + 32375:9380-9399 MS.gene08870:CDS 30.0%
! TGATGTTCTTAGTCTTCTTG+CGG - 32375:9372-9391 MS.gene08870:intergenic 35.0%
ATGTCTTCTCCGATCTTCTT+TGG - 32375:9211-9230 MS.gene08870:intergenic 40.0%
GACGTAAACTTCGACGATTT+GGG - 32375:9119-9138 MS.gene08870:intergenic 40.0%
! TAAACTTCGACGATTTGGGA+TGG - 32375:9115-9134 MS.gene08870:intergenic 40.0%
! TGTTGATGGGAAAGATCCAA+AGG + 32375:9522-9541 MS.gene08870:CDS 40.0%
AGATCCAAAGGATTTGCAGC+AGG + 32375:9534-9553 MS.gene08870:CDS 45.0%
ATCTCCTGCTGCAAATCCTT+TGG - 32375:9541-9560 MS.gene08870:intergenic 45.0%
CAAAATCTCCTTCACCGTTC+CGG - 32375:9476-9495 MS.gene08870:intergenic 45.0%
CAAGGATTGGAAGGGTCTTA+GGG + 32375:9246-9265 MS.gene08870:CDS 45.0%
CTGTTGGGTGTACTGTTGAT+GGG + 32375:9509-9528 MS.gene08870:CDS 45.0%
CTTAGCCTGACGATTCTGAA+CGG - 32375:9287-9306 MS.gene08870:intergenic 45.0%
GGACGTAAACTTCGACGATT+TGG - 32375:9120-9139 MS.gene08870:intergenic 45.0%
GGTCTCTCACCAAAGAAGAT+CGG + 32375:9199-9218 MS.gene08870:CDS 45.0%
TCTGTTGGGTGTACTGTTGA+TGG + 32375:9508-9527 MS.gene08870:CDS 45.0%
TTGCAGCAGGAGATTAGTGA+TGG + 32375:9547-9566 MS.gene08870:CDS 45.0%
TTGCGAAGATTATGAAGCCG+AGG + 32375:9431-9450 MS.gene08870:CDS 45.0%
! ATTTGGGATGGATCGAGCTT+TGG - 32375:9103-9122 MS.gene08870:intergenic 45.0%
! GAACGGTGAAGGAGATTTTG+GGG + 32375:9476-9495 MS.gene08870:CDS 45.0%
! GCTGGTTATCAAGGCTTTGA+AGG + 32375:9333-9352 MS.gene08870:CDS 45.0%
! GGAACGGTGAAGGAGATTTT+GGG + 32375:9475-9494 MS.gene08870:CDS 45.0%
! TGGGGACTTGTGTTTCTGTT+GGG + 32375:9494-9513 MS.gene08870:CDS 45.0%
! TGGTGATGTTGAGATTCCTC+AGG + 32375:9567-9586 MS.gene08870:CDS 45.0%
! TTGGGGACTTGTGTTTCTGT+TGG + 32375:9493-9512 MS.gene08870:CDS 45.0%
!! AGGCTTTGAAGGAACCAGAA+AGG + 32375:9344-9363 MS.gene08870:CDS 45.0%
!! GGCTTTGAAGGAACCAGAAA+GGG + 32375:9345-9364 MS.gene08870:CDS 45.0%
AAGACCCTTCCAATCCTTGG+CGG - 32375:9245-9264 MS.gene08869:intergenic 50.0%
AGAAACCGCCAAGGATTGGA+AGG + 32375:9237-9256 MS.gene08870:CDS 50.0%
CCAAAGAAACCGCCAAGGAT+TGG + 32375:9233-9252 MS.gene08870:CDS 50.0%
CCAAGGATTGGAAGGGTCTT+AGG + 32375:9245-9264 MS.gene08870:CDS 50.0%
CCAATCCTTGGCGGTTTCTT+TGG - 32375:9236-9255 MS.gene08870:intergenic 50.0%
CCTAAGACCCTTCCAATCCT+TGG - 32375:9248-9267 MS.gene08870:intergenic 50.0%
CGTTCAGAATCGTCAGGCTA+AGG + 32375:9285-9304 MS.gene08870:CDS 50.0%
GAAACCGCCAAGGATTGGAA+GGG + 32375:9238-9257 MS.gene08870:CDS 50.0%
GATTATGAAGCCGAGGTCGA+TGG + 32375:9438-9457 MS.gene08870:CDS 50.0%
TTCTTTGGTGAGAGACCGAG+AGG - 32375:9196-9215 MS.gene08870:intergenic 50.0%
! CGGAACGGTGAAGGAGATTT+TGG + 32375:9474-9493 MS.gene08870:CDS 50.0%
AGACCGAGAGGACCGATCTT+GGG - 32375:9184-9203 MS.gene08870:intergenic 55.0%
AGTTCCCTCGCTCCCAAGAT+CGG + 32375:9169-9188 MS.gene08870:CDS 55.0%
CATCGCCAAAGAAACCGCCA+AGG + 32375:9228-9247 MS.gene08870:CDS 55.0%
GAAGTTTACGTCCGCGTCAC+CGG + 32375:9127-9146 MS.gene08870:CDS 55.0%
GCTCACCGTTCAGAATCGTC+AGG + 32375:9279-9298 MS.gene08870:CDS 55.0%
GTCTTCTTGCGGTCCCTTTC+TGG - 32375:9361-9380 MS.gene08870:intergenic 55.0%
! TGGGATGGATCGAGCTTTGG+CGG - 32375:9100-9119 MS.gene08870:intergenic 55.0%
AGCTCCTTCGCCATCGACCT+CGG - 32375:9451-9470 MS.gene08870:intergenic 60.0%
AGGACCGATCTTGGGAGCGA+GGG - 32375:9176-9195 MS.gene08870:intergenic 60.0%
GAGACCGAGAGGACCGATCT+TGG - 32375:9185-9204 MS.gene08870:intergenic 60.0%
GATCTTGGGAGCGAGGGAAC+TGG - 32375:9170-9189 MS.gene08870:intergenic 60.0%
GCTCCCAAGATCGGTCCTCT+CGG + 32375:9178-9197 MS.gene08870:CDS 60.0%
GGTCGCTGTTGTTCCGTCTG+CGG + 32375:9306-9325 MS.gene08870:CDS 60.0%
TCGATGGCGAAGGAGCTTGC+CGG + 32375:9454-9473 MS.gene08870:CDS 60.0%
ATAACCAGCGCCGCCGCAGA+CGG - 32375:9322-9341 MS.gene08870:intergenic 65.0%
CGCTGTTGTTCCGTCTGCGG+CGG + 32375:9309-9328 MS.gene08870:CDS 65.0%
GAAGCCGAGGTCGATGGCGA+AGG + 32375:9444-9463 MS.gene08870:CDS 65.0%
GAGGACCGATCTTGGGAGCG+AGG - 32375:9177-9196 MS.gene08870:intergenic 65.0%
GGAGCTTGCCGGAACGGTGA+AGG + 32375:9465-9484 MS.gene08870:CDS 65.0%
GGCGAAGGAGCTTGCCGGAA+CGG + 32375:9459-9478 MS.gene08870:CDS 65.0%
GTTTACGTCCGCGTCACCGG+CGG + 32375:9130-9149 MS.gene08870:CDS 65.0%
!! TGCGGCGGCGCTGGTTATCA+AGG + 32375:9324-9343 MS.gene08870:CDS 65.0%
CGACTTCACCGCCGGTGACG+CGG - 32375:9141-9160 MS.gene08870:intergenic 70.0%
CGCGTCACCGGCGGTGAAGT+CGG + 32375:9139-9158 MS.gene08870:CDS 70.0%
GGCAGCTCCGACTTCACCGC+CGG - 32375:9149-9168 MS.gene08870:intergenic 70.0%
TGTTCCGTCTGCGGCGGCGC+TGG + 32375:9315-9334 MS.gene08870:CDS 75.0%


Chromosome Type Strat End Strand Name
32375 gene 9094 9594 9094 ID=MS.gene08870
32375 mRNA 9094 9594 9094 ID=MS.gene08870.t1;Parent=MS.gene08870
32375 exon 9094 9594 9094 ID=MS.gene08870.t1.exon1;Parent=MS.gene08870.t1
32375 CDS 9094 9594 9094 ID=cds.MS.gene08870.t1;Parent=MS.gene08870.t1
Gene Sequence

>MS.gene08870

ATGCCGCCAAAGCTCGATCCATCCCAAATCGTCGAAGTTTACGTCCGCGTCACCGGCGGTGAAGTCGGAGCTGCCAGTTCCCTCGCTCCCAAGATCGGTCCTCTCGGTCTCTCACCAAAGAAGATCGGAGAAGACATCGCCAAAGAAACCGCCAAGGATTGGAAGGGTCTTAGGGTGACAGTCAAGCTCACCGTTCAGAATCGTCAGGCTAAGGTCGCTGTTGTTCCGTCTGCGGCGGCGCTGGTTATCAAGGCTTTGAAGGAACCAGAAAGGGACCGCAAGAAGACTAAGAACATCAAGCATAATGGGAATATCTCTCTTGATGATGTTATTGAGATTGCGAAGATTATGAAGCCGAGGTCGATGGCGAAGGAGCTTGCCGGAACGGTGAAGGAGATTTTGGGGACTTGTGTTTCTGTTGGGTGTACTGTTGATGGGAAAGATCCAAAGGATTTGCAGCAGGAGATTAGTGATGGTGATGTTGAGATTCCTCAGGATTGA

Protein sequence

>MS.gene08870.t1

MPPKLDPSQIVEVYVRVTGGEVGAASSLAPKIGPLGLSPKKIGEDIAKETAKDWKGLRVTVKLTVQNRQAKVAVVPSAAALVIKALKEPERDRKKTKNIKHNGNISLDDVIEIAKIMKPRSMAKELAGTVKEILGTCVSVGCTVDGKDPKDLQQEISDGDVEIPQD