AlfalfaGEDB | MS.gene029840

M. sativa cultivar XinJiangDaYe / MS.gene029840

KEGG: K02870
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0019538 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576
PFAM: PF00298.22 PF03946.17

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene029840.t1	XP_013468200.1	100	166	0	0	1	166	1	166	2.10E-84	321.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene029840.t1	O50003	93.4	166	11	0	1	166	1	166	9.3e-81	300.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene029840.t1	I3SXZ6	100.0	166	0	0	1	166	1	166	1.5e-84	321.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene007661	MS.gene029840	PPI
MS.gene037324	MS.gene029840	PPI
MS.gene75537	MS.gene029840	PPI
MS.gene029840	MS.gene001986	PPI
MS.gene99744	MS.gene029840	PPI
MS.gene029242	MS.gene029840	PPI
MS.gene08870	MS.gene029840	PPI
MS.gene045180	MS.gene029840	PPI
MS.gene007662	MS.gene029840	PPI
MS.gene08871	MS.gene029840	PPI
MS.gene029840	MS.gene054186	PPI
MS.gene029840	MS.gene039110	PPI
MS.gene029840	MS.gene63721	PPI
MS.gene08636	MS.gene029840	PPI
MS.gene45417	MS.gene029840	PPI
MS.gene056257	MS.gene029840	PPI
MS.gene029840	MS.gene24598	PPI
MS.gene063366	MS.gene029840	PPI
MS.gene029840	MS.gene067072	PPI
MS.gene060216	MS.gene029840	PPI
MS.gene41916	MS.gene029840	PPI
MS.gene029840	MS.gene050134	PPI
MS.gene066163	MS.gene029840	PPI
MS.gene059422	MS.gene029840	PPI
MS.gene040581	MS.gene029840	PPI
MS.gene050134	MS.gene029840	PPI

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene029840.t1	MTR_1g064500	100.000	166	0	1	166	1	166	1.17e-116	327
MS.gene029840.t1	MTR_4g059400	99.390	164	1	1	164	1	164	1.21e-114	322
MS.gene029840.t1	MTR_2g094300	83.537	164	27	1	164	1	164	1.22e-97	279

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene029840.t1	AT5G60670	90.964	166	15	1	166	1	166	1.27e-107	304
MS.gene029840.t1	AT2G37190	90.854	164	15	1	164	1	164	5.76e-107	303
MS.gene029840.t1	AT3G53430	90.244	164	16	1	164	1	164	1.10e-106	302

Find 59 sgRNAs with CRISPR-Local

Find 59 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CCAGTCCTTTGCTGTTTCTT+TGG	0.201208	1.2:-49619426	None:intergenic
CAACCTTCGCTTGACGATTC+TGG	0.213514	1.2:-49619481	None:intergenic
CGCGGCGGCGTTGGTGATTA+AGG	0.265025	1.2:+49619517	MS.gene029840:CDS
ATGTCTTCTCCGATCTTCTT+TGG	0.296410	1.2:-49619401	None:intergenic
ACGAAGAATATTAAGCATAA+TGG	0.343409	1.2:+49619572	MS.gene029840:CDS
ATCTGTGATGGGTCGAGCTT+TGG	0.352336	1.2:-49619293	None:intergenic
CGAAGAATATTAAGCATAAT+GGG	0.358983	1.2:+49619573	MS.gene029840:CDS
TCTGTTGGGTGTACTGTTGA+TGG	0.361476	1.2:+49619701	MS.gene029840:CDS
AATCTCTTGTTGCAAATCCT+TGG	0.364185	1.2:-49619732	None:intergenic
CTTGGAACTTGTGTTTCTGT+TGG	0.373065	1.2:+49619686	MS.gene029840:CDS
GATTATGAAGCCAAGATCTA+TGG	0.378103	1.2:+49619631	MS.gene029840:CDS
TGAAGGAACCGGAGAGAGAT+AGG	0.383406	1.2:+49619543	MS.gene029840:CDS
ATCTCTTGTTGCAAATCCTT+GGG	0.391437	1.2:-49619731	None:intergenic
TTGGAACTTGTGTTTCTGTT+GGG	0.400252	1.2:+49619687	MS.gene029840:CDS
AGCTCCTTCGCCATAGATCT+TGG	0.402065	1.2:-49619641	None:intergenic
GTTGGTGATTAAGGCGTTGA+AGG	0.418116	1.2:+49619526	MS.gene029840:CDS
AGACCGAGGGGACCGATCTT+GGG	0.428565	1.2:-49619374	None:intergenic
GAGACCGAGGGGACCGATCT+TGG	0.433374	1.2:-49619375	None:intergenic
GGTACTGTGAAGGAAATTCT+TGG	0.448182	1.2:+49619668	MS.gene029840:CDS
GATCTTGGGAGCGAGGGAAC+TGG	0.450092	1.2:-49619360	None:intergenic
TGTGATGGGTCGAGCTTTGG+AGG	0.459117	1.2:-49619290	None:intergenic
TGTTCCCTCCGCGGCGGCGT+TGG	0.461032	1.2:+49619508	MS.gene029840:CDS
GTCTTCTTCCTATCTCTCTC+CGG	0.464028	1.2:-49619551	None:intergenic
GAAGTCTACGTCCGCGTCAC+CGG	0.490300	1.2:+49619320	MS.gene029840:CDS
AGTTCCCTCGCTCCCAAGAT+CGG	0.497799	1.2:+49619362	MS.gene029840:CDS
TCTATGGCGAAGGAGCTTGC+GGG	0.499893	1.2:+49619647	MS.gene029840:CDS
GGCGGCGCCGACTTCTCCTC+CGG	0.502694	1.2:-49619339	None:intergenic
CTGTTGGGTGTACTGTTGAT+GGG	0.503488	1.2:+49619702	MS.gene029840:CDS
CCAAAGAAACAGCAAAGGAC+TGG	0.506670	1.2:+49619426	MS.gene029840:CDS
GATTAAGGCGTTGAAGGAAC+CGG	0.507910	1.2:+49619532	MS.gene029840:CDS
GGGACCGATCTTGGGAGCGA+GGG	0.509084	1.2:-49619366	None:intergenic
ATCTATGGCGAAGGAGCTTG+CGG	0.512673	1.2:+49619646	MS.gene029840:CDS
GGGGACCGATCTTGGGAGCG+AGG	0.533033	1.2:-49619367	None:intergenic
GCTCCCAAGATCGGTCCCCT+CGG	0.536064	1.2:+49619371	MS.gene029840:CDS
CGCGTCACCGGAGGAGAAGT+CGG	0.541002	1.2:+49619332	MS.gene029840:CDS
ATCACCAACGCCGCCGCGGA+GGG	0.564082	1.2:-49619512	None:intergenic
AGAAACAGCAAAGGACTGGA+AGG	0.568635	1.2:+49619430	MS.gene029840:CDS
TTGCAACAAGAGATTGATGA+TGG	0.571171	1.2:+49619740	MS.gene029840:CDS
CGACTTCTCCTCCGGTGACG+CGG	0.575053	1.2:-49619331	None:intergenic
CTTGGGAGCGAGGGAACTGG+CGG	0.575994	1.2:-49619357	None:intergenic
GGTCTCTCACCAAAGAAGAT+CGG	0.586947	1.2:+49619392	MS.gene029840:CDS
TAGACTTCGACGATCTGTGA+TGG	0.587094	1.2:-49619305	None:intergenic
TCTTCTTTGGTGAGAGACCG+AGG	0.587632	1.2:-49619388	None:intergenic
CTTCTTTGGTGAGAGACCGA+GGG	0.589378	1.2:-49619387	None:intergenic
GGAGCTTGCGGGTACTGTGA+AGG	0.597173	1.2:+49619658	MS.gene029840:CDS
AATCACCAACGCCGCCGCGG+AGG	0.602136	1.2:-49619513	None:intergenic
GTCTACGTCCGCGTCACCGG+AGG	0.605485	1.2:+49619323	MS.gene029840:CDS
AGACTTCGACGATCTGTGAT+GGG	0.618253	1.2:-49619304	None:intergenic
TGCTGTTGTTCCCTCCGCGG+CGG	0.624376	1.2:+49619502	MS.gene029840:CDS
CTTCGCTTGACGATTCTGGA+CGG	0.629414	1.2:-49619477	None:intergenic
TGTTGATGGGAAAGATCCCA+AGG	0.632585	1.2:+49619715	MS.gene029840:CDS
CGTCCAGAATCGTCAAGCGA+AGG	0.633297	1.2:+49619478	MS.gene029840:CDS
GAAGCCAAGATCTATGGCGA+AGG	0.638721	1.2:+49619637	MS.gene029840:CDS
GGTTGCTGTTGTTCCCTCCG+CGG	0.642626	1.2:+49619499	MS.gene029840:CDS
GAAACAGCAAAGGACTGGAA+GGG	0.644970	1.2:+49619431	MS.gene029840:CDS
CTTAATCACCAACGCCGCCG+CGG	0.673186	1.2:-49619516	None:intergenic
TTCTTTGGTGAGAGACCGAG+GGG	0.678841	1.2:-49619386	None:intergenic
CATCGCCAAAGAAACAGCAA+AGG	0.684501	1.2:+49619421	MS.gene029840:CDS
CGGTGAGCTTGACTGTGACA+CGG	0.697095	1.2:-49619457	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	ACGAAGAATATTAAGCATAA+TGG	+	chr1.2:49619572-49619591	MS.gene029840:CDS	25.0%
!	CGAAGAATATTAAGCATAAT+GGG	+	chr1.2:49619573-49619592	MS.gene029840:CDS	25.0%
	AATCTCTTGTTGCAAATCCT+TGG	-	chr1.2:49619735-49619754	None:intergenic	35.0%
	ATCTCTTGTTGCAAATCCTT+GGG	-	chr1.2:49619734-49619753	None:intergenic	35.0%
	GATTATGAAGCCAAGATCTA+TGG	+	chr1.2:49619631-49619650	MS.gene029840:CDS	35.0%
	TTGCAACAAGAGATTGATGA+TGG	+	chr1.2:49619740-49619759	MS.gene029840:CDS	35.0%
!	TTGGAACTTGTGTTTCTGTT+GGG	+	chr1.2:49619687-49619706	MS.gene029840:CDS	35.0%
	ATGTCTTCTCCGATCTTCTT+TGG	-	chr1.2:49619404-49619423	None:intergenic	40.0%
	GGTACTGTGAAGGAAATTCT+TGG	+	chr1.2:49619668-49619687	MS.gene029840:CDS	40.0%
!	CTTGGAACTTGTGTTTCTGT+TGG	+	chr1.2:49619686-49619705	MS.gene029840:CDS	40.0%
	AGAAACAGCAAAGGACTGGA+AGG	+	chr1.2:49619430-49619449	MS.gene029840:CDS	45.0%
	AGACTTCGACGATCTGTGAT+GGG	-	chr1.2:49619307-49619326	None:intergenic	45.0%
	CATCGCCAAAGAAACAGCAA+AGG	+	chr1.2:49619421-49619440	MS.gene029840:CDS	45.0%
	CCAAAGAAACAGCAAAGGAC+TGG	+	chr1.2:49619426-49619445	MS.gene029840:CDS	45.0%
	CTGTTGGGTGTACTGTTGAT+GGG	+	chr1.2:49619702-49619721	MS.gene029840:CDS	45.0%
	GAAACAGCAAAGGACTGGAA+GGG	+	chr1.2:49619431-49619450	MS.gene029840:CDS	45.0%
	GGTCTCTCACCAAAGAAGAT+CGG	+	chr1.2:49619392-49619411	MS.gene029840:CDS	45.0%
	GTCTTCTTCCTATCTCTCTC+CGG	-	chr1.2:49619554-49619573	None:intergenic	45.0%
	TAGACTTCGACGATCTGTGA+TGG	-	chr1.2:49619308-49619327	None:intergenic	45.0%
	TCTGTTGGGTGTACTGTTGA+TGG	+	chr1.2:49619701-49619720	MS.gene029840:CDS	45.0%
!	TGTTGATGGGAAAGATCCCA+AGG	+	chr1.2:49619715-49619734	MS.gene029840:CDS	45.0%
!!	CCAGTCCTTTGCTGTTTCTT+TGG	-	chr1.2:49619429-49619448	None:intergenic	45.0%
!!	GATTAAGGCGTTGAAGGAAC+CGG	+	chr1.2:49619532-49619551	MS.gene029840:CDS	45.0%
!!	GTTGGTGATTAAGGCGTTGA+AGG	+	chr1.2:49619526-49619545	MS.gene029840:CDS	45.0%
	AGCTCCTTCGCCATAGATCT+TGG	-	chr1.2:49619644-49619663	None:intergenic	50.0%
	ATCTATGGCGAAGGAGCTTG+CGG	+	chr1.2:49619646-49619665	MS.gene029840:CDS	50.0%
	CAACCTTCGCTTGACGATTC+TGG	-	chr1.2:49619484-49619503	None:intergenic	50.0%
	CTTCGCTTGACGATTCTGGA+CGG	-	chr1.2:49619480-49619499	None:intergenic	50.0%
	CTTCTTTGGTGAGAGACCGA+GGG	-	chr1.2:49619390-49619409	None:intergenic	50.0%
	GAAGCCAAGATCTATGGCGA+AGG	+	chr1.2:49619637-49619656	MS.gene029840:CDS	50.0%
	TCTTCTTTGGTGAGAGACCG+AGG	-	chr1.2:49619391-49619410	None:intergenic	50.0%
	TGAAGGAACCGGAGAGAGAT+AGG	+	chr1.2:49619543-49619562	MS.gene029840:CDS	50.0%
	TTCTTTGGTGAGAGACCGAG+GGG	-	chr1.2:49619389-49619408	None:intergenic	50.0%
!!	ATCTGTGATGGGTCGAGCTT+TGG	-	chr1.2:49619296-49619315	None:intergenic	50.0%
	AGTTCCCTCGCTCCCAAGAT+CGG	+	chr1.2:49619362-49619381	MS.gene029840:CDS	55.0%
	CGGTGAGCTTGACTGTGACA+CGG	-	chr1.2:49619460-49619479	None:intergenic	55.0%
	CGTCCAGAATCGTCAAGCGA+AGG	+	chr1.2:49619478-49619497	MS.gene029840:CDS	55.0%
	TCTATGGCGAAGGAGCTTGC+GGG	+	chr1.2:49619647-49619666	MS.gene029840:CDS	55.0%
!!	TGTGATGGGTCGAGCTTTGG+AGG	-	chr1.2:49619293-49619312	None:intergenic	55.0%
	AGACCGAGGGGACCGATCTT+GGG	-	chr1.2:49619377-49619396	None:intergenic	60.0%
	CTTAATCACCAACGCCGCCG+CGG	-	chr1.2:49619519-49619538	None:intergenic	60.0%
	GAAGTCTACGTCCGCGTCAC+CGG	+	chr1.2:49619320-49619339	MS.gene029840:CDS	60.0%
	GATCTTGGGAGCGAGGGAAC+TGG	-	chr1.2:49619363-49619382	None:intergenic	60.0%
!	GGAGCTTGCGGGTACTGTGA+AGG	+	chr1.2:49619658-49619677	MS.gene029840:CDS	60.0%
!	GGTTGCTGTTGTTCCCTCCG+CGG	+	chr1.2:49619499-49619518	MS.gene029840:CDS	60.0%
	CGACTTCTCCTCCGGTGACG+CGG	-	chr1.2:49619334-49619353	None:intergenic	65.0%
	CGCGTCACCGGAGGAGAAGT+CGG	+	chr1.2:49619332-49619351	MS.gene029840:CDS	65.0%
	CTTGGGAGCGAGGGAACTGG+CGG	-	chr1.2:49619360-49619379	None:intergenic	65.0%
	GAGACCGAGGGGACCGATCT+TGG	-	chr1.2:49619378-49619397	None:intergenic	65.0%
	GCTCCCAAGATCGGTCCCCT+CGG	+	chr1.2:49619371-49619390	MS.gene029840:CDS	65.0%
	GGGACCGATCTTGGGAGCGA+GGG	-	chr1.2:49619369-49619388	None:intergenic	65.0%
	TGCTGTTGTTCCCTCCGCGG+CGG	+	chr1.2:49619502-49619521	MS.gene029840:CDS	65.0%
!!	CGCGGCGGCGTTGGTGATTA+AGG	+	chr1.2:49619517-49619536	MS.gene029840:CDS	65.0%
	AATCACCAACGCCGCCGCGG+AGG	-	chr1.2:49619516-49619535	None:intergenic	70.0%
	ATCACCAACGCCGCCGCGGA+GGG	-	chr1.2:49619515-49619534	None:intergenic	70.0%
	GGGGACCGATCTTGGGAGCG+AGG	-	chr1.2:49619370-49619389	None:intergenic	70.0%
	GTCTACGTCCGCGTCACCGG+AGG	+	chr1.2:49619323-49619342	MS.gene029840:CDS	70.0%
	TGTTCCCTCCGCGGCGGCGT+TGG	+	chr1.2:49619508-49619527	MS.gene029840:CDS	75.0%
!!	GGCGGCGCCGACTTCTCCTC+CGG	-	chr1.2:49619342-49619361	None:intergenic	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.2	gene	49619287	49619787	49619287	ID=MS.gene029840
chr1.2	mRNA	49619287	49619787	49619287	ID=MS.gene029840.t1;Parent=MS.gene029840
chr1.2	exon	49619287	49619787	49619287	ID=MS.gene029840.t1.exon1;Parent=MS.gene029840.t1
chr1.2	CDS	49619287	49619787	49619287	ID=cds.MS.gene029840.t1;Parent=MS.gene029840.t1

Gene Sequence

>MS.gene029840

ATGCCTCCAAAGCTCGACCCATCACAGATCGTCGAAGTCTACGTCCGCGTCACCGGAGGAGAAGTCGGCGCCGCCAGTTCCCTCGCTCCCAAGATCGGTCCCCTCGGTCTCTCACCAAAGAAGATCGGAGAAGACATCGCCAAAGAAACAGCAAAGGACTGGAAGGGTCTCCGTGTCACAGTCAAGCTCACCGTCCAGAATCGTCAAGCGAAGGTTGCTGTTGTTCCCTCCGCGGCGGCGTTGGTGATTAAGGCGTTGAAGGAACCGGAGAGAGATAGGAAGAAGACGAAGAATATTAAGCATAATGGGAATATCTCACTTGATGATGTTATTGAGATTGCGAAGATTATGAAGCCAAGATCTATGGCGAAGGAGCTTGCGGGTACTGTGAAGGAAATTCTTGGAACTTGTGTTTCTGTTGGGTGTACTGTTGATGGGAAAGATCCCAAGGATTTGCAACAAGAGATTGATGATGGTGATGTTGAGATTCCTTTAGAATAA

Protein sequence

>MS.gene029840.t1

MPPKLDPSQIVEVYVRVTGGEVGAASSLAPKIGPLGLSPKKIGEDIAKETAKDWKGLRVTVKLTVQNRQAKVAVVPSAAALVIKALKEPERDRKKTKNIKHNGNISLDDVIEIAKIMKPRSMAKELAGTVKEILGTCVSVGCTVDGKDPKDLQQEIDDGDVEIPLE