AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene01038

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene01038.t1 MTR_2g014470 96.542 347 5 2 1 347 1 340 0.0 676
MS.gene01038.t1 MTR_4g078850 78.797 349 66 4 1 346 1 344 0.0 540
MS.gene01038.t1 MTR_4g015840 69.143 350 93 5 1 346 1 339 3.59e-167 469
MS.gene01038.t1 MTR_2g090705 69.429 350 88 3 1 346 1 335 7.73e-163 458
MS.gene01038.t1 MTR_2g014480 68.895 344 91 6 1 336 1 336 5.02e-152 431
MS.gene01038.t1 MTR_3g108020 55.652 345 133 4 1 345 1 325 1.14e-127 369
MS.gene01038.t1 MTR_4g015840 68.504 254 68 4 97 346 17 262 3.51e-113 330
MS.gene01038.t1 MTR_3g437360 39.169 337 195 7 2 329 3 338 4.67e-69 219
MS.gene01038.t1 MTR_5g037880 54.237 177 81 0 169 345 98 274 4.87e-67 213
MS.gene01038.t1 MTR_5g037880 52.128 94 32 2 4 90 5 92 7.69e-21 91.3
MS.gene01038.t1 MTR_3g437840 51.553 161 78 0 186 346 2 162 2.32e-54 176
MS.gene01038.t1 MTR_7g109340 33.913 345 196 7 5 345 27 343 2.30e-50 171
MS.gene01038.t1 MTR_3g088140 42.857 196 105 3 153 341 157 352 1.28e-44 157
MS.gene01038.t1 MTR_8g006430 33.609 363 204 11 5 345 15 362 1.81e-44 158
MS.gene01038.t1 MTR_2g006890 45.000 180 98 1 167 345 196 375 3.34e-44 156
MS.gene01038.t1 MTR_3g437850 35.610 205 89 5 3 202 1 167 5.38e-32 118
MS.gene01038.t1 MTR_3g087030 27.793 367 216 11 4 344 82 425 1.34e-31 123
MS.gene01038.t1 MTR_2g087660 37.241 145 89 1 203 345 214 358 1.54e-25 106
MS.gene01038.t1 MTR_2g087660 60.563 71 23 2 5 75 14 79 5.33e-12 67.0
MS.gene01038.t1 MTR_4g050420 32.979 188 118 4 161 345 174 356 4.40e-25 105
MS.gene01038.t1 MTR_4g050420 59.155 71 24 2 5 75 14 79 3.99e-11 64.3
MS.gene01038.t1 MTR_5g065160 26.849 365 206 13 4 338 76 409 2.06e-22 98.2
MS.gene01038.t1 MTR_5g065160 26.849 365 206 13 4 338 83 416 2.62e-22 97.8
MS.gene01038.t1 MTR_2g104030 44.697 132 51 4 4 135 70 179 2.67e-21 95.1
MS.gene01038.t1 MTR_3g027580 27.746 346 223 11 4 338 89 418 4.16e-21 94.0
MS.gene01038.t1 MTR_7g101070 60.000 65 26 0 4 68 358 422 5.24e-20 91.3
MS.gene01038.t1 MTR_7g112360 46.970 66 32 1 5 67 17 82 4.33e-16 75.5
MS.gene01038.t1 MTR_7g112360 46.970 66 32 1 5 67 17 82 7.50e-16 73.2
MS.gene01038.t1 MTR_7g069480 51.429 70 32 1 3 72 26 93 8.06e-16 79.0
MS.gene01038.t1 MTR_5g012530 48.649 74 36 1 4 75 12 85 1.02e-15 78.6
MS.gene01038.t1 MTR_2g087660 38.462 91 56 0 203 293 214 304 2.69e-15 76.3
MS.gene01038.t1 MTR_2g087660 60.563 71 23 2 5 75 14 79 3.19e-12 67.0
MS.gene01038.t1 MTR_6g027110 44.000 75 38 1 3 73 1 75 5.70e-15 71.6
MS.gene01038.t1 MTR_5g065860 50.000 64 31 1 4 67 48 110 7.57e-15 71.2
MS.gene01038.t1 MTR_6g471050 24.786 351 206 14 30 346 7 333 1.38e-14 74.7
MS.gene01038.t1 MTR_1g110310 49.296 71 35 1 4 74 16 85 1.72e-14 74.3
MS.gene01038.t1 MTR_6g027030 45.333 75 37 1 3 73 1 75 1.88e-14 72.0
MS.gene01038.t1 MTR_8g022310 44.928 69 33 1 4 67 10 78 3.51e-14 71.2
MS.gene01038.t1 MTR_4g085070 50.000 60 27 1 11 67 19 78 4.89e-14 69.3
MS.gene01038.t1 MTR_8g022310 44.928 69 33 1 4 67 10 78 5.45e-14 70.1
MS.gene01038.t1 MTR_8g022310 43.478 69 34 1 4 67 10 78 5.65e-14 70.5
MS.gene01038.t1 MTR_8g022310 43.478 69 34 1 4 67 10 78 7.29e-14 70.5
MS.gene01038.t1 MTR_5g035770 47.945 73 37 1 4 76 25 96 1.84e-13 71.2
MS.gene01038.t1 MTR_5g065160 48.571 70 35 1 4 73 83 151 1.93e-13 71.2
MS.gene01038.t1 MTR_5g065160 48.571 70 35 1 4 73 82 150 2.07e-13 71.2
MS.gene01038.t1 MTR_6g026940 44.928 69 34 1 3 67 1 69 2.19e-13 68.2
MS.gene01038.t1 MTR_1g052110 47.692 65 33 1 6 70 40 103 2.59e-13 69.7
MS.gene01038.t1 MTR_4g023470 39.167 120 59 4 5 123 87 193 6.65e-13 69.7
MS.gene01038.t1 MTR_2g436560 36.154 130 59 5 4 123 104 219 2.28e-12 67.8
MS.gene01038.t1 MTR_7g092380 42.105 76 40 2 6 78 24 98 4.08e-12 66.2
MS.gene01038.t1 MTR_1g029460 41.667 72 41 1 3 74 5 75 5.62e-12 66.6
MS.gene01038.t1 MTR_5g004570 45.070 71 38 1 4 74 6 75 1.56e-11 63.9
MS.gene01038.t1 MTR_4g094275 41.558 77 40 3 2 75 49 123 9.06e-11 60.5
MS.gene01038.t1 MTR_4g109510 44.118 68 35 1 5 69 9 76 9.19e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene01038.t1 AT2G20560 74.571 350 70 4 1 345 1 336 0.0 516
MS.gene01038.t1 AT4G28480 69.382 356 89 3 1 345 1 347 2.96e-170 478
MS.gene01038.t1 AT3G08910 65.896 346 93 5 1 346 1 321 5.60e-160 451
MS.gene01038.t1 AT5G01390 62.571 350 113 4 1 347 1 335 3.07e-154 436
MS.gene01038.t1 AT4G28480 61.159 345 78 3 1 345 1 289 4.46e-142 404
MS.gene01038.t1 AT5G25530 59.040 354 121 7 1 346 1 338 1.30e-139 399
MS.gene01038.t1 AT5G25530 57.735 362 121 8 1 346 1 346 1.40e-136 392
MS.gene01038.t1 AT1G10350 56.772 347 147 3 1 345 1 346 4.79e-130 375
MS.gene01038.t1 AT3G47940 54.017 361 135 4 1 345 1 346 7.03e-129 373
MS.gene01038.t1 AT1G59725 55.172 348 133 3 1 345 1 328 5.45e-127 367
MS.gene01038.t1 AT2G20550 55.670 291 71 4 103 345 3 283 1.50e-98 293
MS.gene01038.t1 AT2G20550 55.670 291 71 4 103 345 3 283 1.50e-98 293
MS.gene01038.t1 AT5G01390 45.245 347 120 7 1 347 1 277 7.25e-89 268
MS.gene01038.t1 AT5G01390 54.762 210 77 4 1 207 1 195 6.47e-68 213
MS.gene01038.t1 AT5G01390 61.029 136 48 2 1 135 1 132 3.14e-49 165
MS.gene01038.t1 AT5G01390 54.930 142 25 1 206 347 136 238 3.20e-43 149
MS.gene01038.t1 AT1G44160 47.753 178 93 0 168 345 176 353 3.77e-47 163
MS.gene01038.t1 AT3G62600 32.378 349 206 7 2 346 24 346 1.07e-44 157
MS.gene01038.t1 AT1G11040 43.353 173 98 0 166 338 253 425 7.78e-43 154
MS.gene01038.t1 AT3G44110 33.616 354 212 9 5 345 15 358 4.61e-40 146
MS.gene01038.t1 AT5G22060 34.066 364 198 11 5 345 15 359 3.48e-38 141
MS.gene01038.t1 AT3G44110 33.642 324 192 9 5 315 15 328 7.38e-34 128
MS.gene01038.t1 AT1G80030 28.382 377 210 13 4 344 75 427 1.04e-28 116
MS.gene01038.t1 AT1G80030 28.382 377 210 13 4 344 75 427 1.68e-28 115
MS.gene01038.t1 AT1G80030 28.382 377 210 13 4 344 75 427 1.68e-28 115
MS.gene01038.t1 AT1G80030 28.382 377 210 13 4 344 75 427 1.68e-28 115
MS.gene01038.t1 AT2G22360 65.333 75 20 2 4 76 86 156 7.68e-22 96.3
MS.gene01038.t1 AT2G22360 29.412 136 92 3 211 344 295 428 9.02e-12 66.2
MS.gene01038.t1 AT3G17830 52.174 92 38 2 2 91 61 148 1.44e-20 93.2
MS.gene01038.t1 AT4G39960 58.904 73 28 1 4 76 85 155 2.66e-20 92.0
MS.gene01038.t1 AT4G39960 29.412 136 92 3 211 344 301 434 2.73e-12 67.8
MS.gene01038.t1 AT4G39960 58.904 73 28 1 4 76 85 155 2.66e-20 92.0
MS.gene01038.t1 AT4G39960 29.412 136 92 3 211 344 301 434 2.73e-12 67.8
MS.gene01038.t1 AT5G48030 46.018 113 51 3 4 116 94 196 2.16e-17 83.2
MS.gene01038.t1 AT5G16650 59.375 64 25 1 4 67 11 73 6.80e-17 77.8
MS.gene01038.t1 AT5G03160 53.846 65 30 0 4 68 370 434 7.31e-17 82.0
MS.gene01038.t1 AT5G16650 59.375 64 25 1 4 67 11 73 9.44e-17 76.3
MS.gene01038.t1 AT1G59980 47.826 69 35 1 6 74 25 92 1.62e-15 77.0
MS.gene01038.t1 AT2G41000 47.826 69 32 1 3 67 2 70 1.80e-15 74.7
MS.gene01038.t1 AT1G56300 48.485 66 31 1 5 67 14 79 2.51e-15 72.0
MS.gene01038.t1 AT1G56300 48.485 66 31 1 5 67 14 79 2.53e-15 73.2
MS.gene01038.t1 AT1G68370 52.113 71 33 1 4 74 17 86 2.57e-15 77.0
MS.gene01038.t1 AT1G59980 47.826 69 35 1 6 74 25 92 3.93e-15 76.3
MS.gene01038.t1 AT2G41000 47.826 69 32 1 3 67 2 70 6.01e-15 72.8
MS.gene01038.t1 AT3G08970 51.316 76 33 2 2 77 25 96 2.25e-14 74.7
MS.gene01038.t1 AT3G08970 51.316 76 33 2 2 77 25 96 2.25e-14 74.7
MS.gene01038.t1 AT2G35720 47.945 73 36 1 4 74 15 87 3.25e-14 73.9
MS.gene01038.t1 AT2G21510 49.315 73 36 1 3 75 5 76 1.85e-13 70.9
MS.gene01038.t1 AT2G21510 49.315 73 36 1 3 75 5 76 1.85e-13 70.9
MS.gene01038.t1 AT2G21510 49.315 73 36 1 3 75 5 76 2.04e-13 70.9
MS.gene01038.t1 AT2G21510 49.315 73 36 1 3 75 5 76 2.04e-13 70.9
MS.gene01038.t1 AT2G21510 44.828 87 47 1 4 90 6 91 2.41e-13 70.5
MS.gene01038.t1 AT2G33735 43.077 65 36 1 4 68 22 85 1.07e-12 64.7
MS.gene01038.t1 AT2G33735 43.077 65 36 1 4 68 22 85 1.07e-12 64.7
MS.gene01038.t1 AT1G71000 45.455 66 33 1 5 67 9 74 1.60e-12 65.5
MS.gene01038.t1 AT1G24120 44.595 74 40 1 4 77 20 92 1.88e-12 68.6
MS.gene01038.t1 AT5G06910 46.154 65 34 1 6 70 31 94 1.96e-12 67.4
MS.gene01038.t1 AT1G71000 45.455 66 33 1 5 67 9 74 3.45e-12 64.3
MS.gene01038.t1 AT4G39150 47.222 72 37 1 4 75 6 76 6.16e-12 66.2
MS.gene01038.t1 AT4G39150 47.222 72 37 1 4 75 6 76 6.16e-12 66.2
MS.gene01038.t1 AT4G39150 47.222 72 37 1 4 75 6 76 6.16e-12 66.2
MS.gene01038.t1 AT5G05750 36.283 113 61 3 4 116 114 215 6.29e-12 65.9
MS.gene01038.t1 AT3G14200 45.312 64 33 1 6 67 14 77 6.65e-12 65.1
MS.gene01038.t1 AT1G21080 42.857 91 49 2 3 93 5 92 1.55e-11 65.5
MS.gene01038.t1 AT1G21080 42.857 91 49 2 3 93 5 92 1.67e-11 65.5
MS.gene01038.t1 AT3G13310 46.774 62 29 1 6 67 66 123 2.17e-11 62.0
MS.gene01038.t1 AT4G13830 38.806 67 40 1 3 68 65 131 2.95e-11 61.6
MS.gene01038.t1 AT5G49060 46.269 67 34 1 4 70 99 163 3.48e-11 64.3
MS.gene01038.t1 AT5G49060 46.269 67 34 1 4 70 99 163 3.48e-11 64.3
MS.gene01038.t1 AT4G13830 38.806 67 40 1 3 68 65 131 3.99e-11 61.6
MS.gene01038.t1 AT1G72416 34.831 89 49 2 4 90 5 86 6.01e-11 61.6
MS.gene01038.t1 AT3G12170 43.077 65 36 1 6 70 13 76 6.46e-11 62.4

Find 90 sgRNAs with CRISPR-Local

Find 153 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAATAACCTCTTCATAATT+TGG 0.139392 2.4:+70436453 None:intergenic
CCCTTCTTTGAAGGATCTTT+TGG 0.174093 2.4:+70436411 None:intergenic
TTCAGATATCTCTTTGAATT+TGG 0.183628 2.4:+70437968 None:intergenic
TTGACTTCTGAGCAGAAATT+TGG 0.209442 2.4:-70436348 MS.gene01038:CDS
AAGATATCGTTTGCGTTTCT+TGG 0.221316 2.4:+70437701 None:intergenic
GGATGAATCACATTGTTTAT+AGG 0.244976 2.4:+70436474 None:intergenic
TTATTTGAGGGATTCTTATC+AGG 0.267987 2.4:+70438005 None:intergenic
TATGGAGAAGAAGGGCTAAA+AGG 0.276981 2.4:-70437806 MS.gene01038:CDS
GGTTGCTCATTTCCTTTCTC+AGG 0.283976 2.4:+70436663 None:intergenic
TCGGGTATTCAAGCCCGTTT+GGG 0.303828 2.4:-70437667 MS.gene01038:CDS
TGCTCAGAAGTCAACCTTGC+TGG 0.309488 2.4:+70436357 None:intergenic
AGTCAAGCCAGGGTGGAAAA+AGG 0.321865 2.4:-70436703 MS.gene01038:CDS
TTCGGGTATTCAAGCCCGTT+TGG 0.333587 2.4:-70437668 MS.gene01038:CDS
AAAGCAATTTATGATCAATA+TGG 0.348727 2.4:-70437824 MS.gene01038:CDS
ATTTGGAATCAAGAAACTTC+TGG 0.350613 2.4:-70436331 MS.gene01038:CDS
CTTATCAGGGTGCCACTTCA+TGG 0.353470 2.4:+70438019 None:intergenic
CTTCAAAGAAGGGTAATCTT+AGG 0.357619 2.4:-70436401 MS.gene01038:CDS
AGGTAGGCCTTTGCGTCAAC+AGG 0.359224 2.4:-70437540 MS.gene01038:CDS
TACAAGAGATGGCAATGATT+TGG 0.359667 2.4:-70436583 MS.gene01038:CDS
TCACTTGCAGAAGCTTTAAC+AGG 0.361103 2.4:-70436543 MS.gene01038:CDS
CTAAGATTACCCTTCTTTGA+AGG 0.367122 2.4:+70436402 None:intergenic
AGCCTTGAGGAACTCTATAA+AGG 0.369722 2.4:-70437476 MS.gene01038:CDS
AGATCTGCTGGTATCACATT+TGG 0.371997 2.4:+70436642 None:intergenic
AGATCCTTTGGTGGAATGTT+TGG 0.375285 2.4:-70437596 MS.gene01038:CDS
CTCAGCGTCTTTCTTATTTG+AGG 0.384413 2.4:+70437992 None:intergenic
GGAATAGGCATACCTTCCTT+TGG 0.388950 2.4:+70436432 None:intergenic
GAGATGGACCAACAACGTTT+AGG 0.400923 2.4:-70437730 MS.gene01038:CDS
AGAGGTGGAGCCAGATCCTT+TGG 0.409550 2.4:-70437608 MS.gene01038:CDS
GCCTTGAGGAACTCTATAAA+GGG 0.442299 2.4:-70437475 MS.gene01038:CDS
AAACTGTAATGTTACCTCAT+AGG 0.442550 2.4:+70437944 None:intergenic
TATGATCAATATGGAGAAGA+AGG 0.445033 2.4:-70437815 MS.gene01038:CDS
TCAGCGTCTTTCTTATTTGA+GGG 0.447077 2.4:+70437993 None:intergenic
ATGGAGAAGAAGGGCTAAAA+GGG 0.457737 2.4:-70437805 MS.gene01038:CDS
TTAACAATTGAAGTCAAGCC+AGG 0.465128 2.4:-70436714 MS.gene01038:CDS
TCCAAAAGATCCTTCAAAGA+AGG 0.470055 2.4:-70436412 MS.gene01038:CDS
CACCTCAAGATGCTGCTGGT+GGG 0.471293 2.4:-70437772 MS.gene01038:CDS
CTTCTTCAACTCTTCTTCTG+TGG 0.473979 2.4:+70438058 None:intergenic
GTTGCTCATTTCCTTTCTCA+GGG 0.474664 2.4:+70436664 None:intergenic
TATTTGAGGGATTCTTATCA+GGG 0.478544 2.4:+70438006 None:intergenic
GGATTGAACCTAAACGTTGT+TGG 0.479222 2.4:+70437722 None:intergenic
TCCTTTGGTGGAATGTTTGG+TGG 0.483436 2.4:-70437593 MS.gene01038:CDS
GTTCGTCTCACAACACTAGA+TGG 0.485111 2.4:-70436513 MS.gene01038:CDS
ATAACAAATATAAGATCTGC+TGG 0.490598 2.4:+70436630 None:intergenic
AAACATTCCACCAAAGGATC+TGG 0.493654 2.4:+70437598 None:intergenic
GTCTCACAACACTAGATGGC+AGG 0.501582 2.4:-70436509 MS.gene01038:CDS
AAAATCACATTCCCTGAGAA+AGG 0.501962 2.4:-70436675 MS.gene01038:CDS
ATTGTTAAGATTTCCTCCAC+TGG 0.504925 2.4:+70436729 None:intergenic
GCTGGTGGGAATGGAATGAG+AGG 0.505271 2.4:-70437626 MS.gene01038:CDS
ACCTCAAGATGCTGCTGGTG+GGG 0.512127 2.4:-70437771 MS.gene01038:CDS
ACATTCTTCCAAAGTGGAGA+TGG 0.515684 2.4:-70437746 MS.gene01038:CDS
CCAAAAGATCCTTCAAAGAA+GGG 0.516873 2.4:-70436411 MS.gene01038:CDS
CATAGTGTATTTACAAGAGA+TGG 0.519390 2.4:-70436594 MS.gene01038:CDS
TAGAGTTCCTCAAGGCTACA+AGG 0.520626 2.4:+70437482 None:intergenic
AGAGATATCTGAAGCCTATG+AGG 0.524531 2.4:-70437958 MS.gene01038:intron
CGAGGACCCTGTTGACGCAA+AGG 0.535042 2.4:+70437533 None:intergenic
CCACCTCAAGATGCTGCTGG+TGG 0.539888 2.4:-70437773 MS.gene01038:CDS
CCAGCAGCATCTTGAGGTGG+TGG 0.542898 2.4:+70437776 None:intergenic
ATCGAAAACTTGCCATGAAG+TGG 0.546006 2.4:-70438031 MS.gene01038:CDS
TTGTTGGTCCATCTCCACTT+TGG 0.559606 2.4:+70437738 None:intergenic
TTTCTTCATTTGATGAAGGT+AGG 0.563556 2.4:-70437556 MS.gene01038:CDS
GCCCCACCAGCAGCATCTTG+AGG 0.565292 2.4:+70437770 None:intergenic
TCAATAGCAGCTGCCTTGCG+AGG 0.571839 2.4:+70437515 None:intergenic
CACAGAAGAAGAGTTGAAGA+AGG 0.577915 2.4:-70438057 MS.gene01038:CDS
TTATGAAGAGGTTATTCCAA+AGG 0.579708 2.4:-70436448 MS.gene01038:CDS
AGGGAAATTGCTGATGCAAG+TGG 0.584073 2.4:-70437428 MS.gene01038:CDS
CGGGTATTCAAGCCCGTTTG+GGG 0.586346 2.4:-70437666 MS.gene01038:CDS
GCGTCAACAGGGTCCTCGCA+AGG 0.592745 2.4:-70437528 MS.gene01038:CDS
GGGAAATTGCTGATGCAAGT+GGG 0.597387 2.4:-70437427 MS.gene01038:intron
TCTCTTGTAAATACACTATG+TGG 0.600191 2.4:+70436597 None:intergenic
GAAGAGGTTATTCCAAAGGA+AGG 0.600896 2.4:-70436444 MS.gene01038:CDS
TTAATATCAAGTTTCCAGCA+AGG 0.601452 2.4:-70436371 MS.gene01038:CDS
GATTCATCCAAATTATGAAG+AGG 0.602330 2.4:-70436460 MS.gene01038:CDS
TAGATTGCCTTGTAGCCTTG+AGG 0.603463 2.4:-70437489 MS.gene01038:CDS
CCACCACCTCAAGATGCTGC+TGG 0.609111 2.4:-70437776 MS.gene01038:CDS
GGTGGAGCCAGATCCTTTGG+TGG 0.611596 2.4:-70437605 MS.gene01038:CDS
AGAAACTTCTGGCACCGTAG+TGG 0.613726 2.4:-70436320 None:intergenic
ATGATCAATATGGAGAAGAA+GGG 0.615681 2.4:-70437814 MS.gene01038:CDS
GGTGGGAATGGAATGAGAGG+TGG 0.620935 2.4:-70437623 MS.gene01038:CDS
GGGGCTACATTCTTCCAAAG+TGG 0.625228 2.4:-70437752 MS.gene01038:CDS
CCACCAGCAGCATCTTGAGG+TGG 0.626886 2.4:+70437773 None:intergenic
ACCACCAAACATTCCACCAA+AGG 0.627179 2.4:+70437592 None:intergenic
CAGGAAAACTATGCCAGTGG+AGG 0.633181 2.4:-70436742 MS.gene01038:CDS
TTGCAGGAAAACTATGCCAG+TGG 0.635655 2.4:-70436745 MS.gene01038:intron
TCCCTTTATAGAGTTCCTCA+AGG 0.641392 2.4:+70437474 None:intergenic
TCTCACAACACTAGATGGCA+GGG 0.644540 2.4:-70436508 MS.gene01038:CDS
TAACAATTGAAGTCAAGCCA+GGG 0.646777 2.4:-70436713 MS.gene01038:CDS
CAATTGAAGTCAAGCCAGGG+TGG 0.649151 2.4:-70436710 MS.gene01038:CDS
GGGTATTCAAGCCCGTTTGG+GGG 0.650237 2.4:-70437665 MS.gene01038:CDS
CTCAAATAAGAAAGACGCTG+AGG 0.667226 2.4:-70437991 MS.gene01038:CDS
GGTAGGCCTTTGCGTCAACA+GGG 0.671612 2.4:-70437539 MS.gene01038:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCAATAATAATTTAAAAAAC+TGG - chr2.4:70437333-70437352 MS.gene01038:intron 10.0%
!! AAAGCAATTTATGATCAATA+TGG - chr2.4:70436600-70436619 MS.gene01038:CDS 20.0%
!! ACAACTTGCAATTTAAAAAT+CGG + chr2.4:70437547-70437566 None:intergenic 20.0%
!! TAGAAATTCTGAATATTAAC+TGG - chr2.4:70436537-70436556 MS.gene01038:CDS 20.0%
!! TCTAAATTATTTCAAAAAGC+AGG + chr2.4:70437143-70437162 None:intergenic 20.0%
! AAAAGTTGAACAGTTGAATT+TGG - chr2.4:70437445-70437464 MS.gene01038:CDS 25.0%
! ATAACAAATATAAGATCTGC+TGG + chr2.4:70437797-70437816 None:intergenic 25.0%
! ATAATTTAGATGATGAAGCA+CGG - chr2.4:70437269-70437288 MS.gene01038:intron 25.0%
! ATTGTTACATCACTATATGT+TGG - chr2.4:70437044-70437063 MS.gene01038:intron 25.0%
! CAAAAAGATGAAGATTTCAA+GGG - chr2.4:70436977-70436996 MS.gene01038:intron 25.0%
! CTAATTATTTATCTCTTTGC+AGG - chr2.4:70437663-70437682 MS.gene01038:CDS 25.0%
! CTGCAAAGAGATAAATAATT+AGG + chr2.4:70437665-70437684 None:intergenic 25.0%
! TCAGAATTTCTATGAAGAAT+TGG + chr2.4:70436529-70436548 None:intergenic 25.0%
! TTAGATTCCTCAAAATATGT+TGG - chr2.4:70437070-70437089 MS.gene01038:intron 25.0%
! TTCAGATATCTCTTTGAATT+TGG + chr2.4:70436459-70436478 None:intergenic 25.0%
!! ATGTTTTCTTCATTTGATGA+AGG - chr2.4:70436864-70436883 MS.gene01038:intron 25.0%
!! ATTAGATCCAACATATTTTG+AGG + chr2.4:70437080-70437099 None:intergenic 25.0%
!! TCATAAATTGCTTTCTTTTG+TGG + chr2.4:70436594-70436613 None:intergenic 25.0%
!!! AAATTGCAAGTTGTTTTTGT+GGG - chr2.4:70437553-70437572 MS.gene01038:CDS 25.0%
!!! TAAATTGCAAGTTGTTTTTG+TGG - chr2.4:70437552-70437571 MS.gene01038:CDS 25.0%
!!! TTCTGAATATTAACTGGTTT+TGG - chr2.4:70436543-70436562 MS.gene01038:CDS 25.0%
AAACTGTAATGTTACCTCAT+AGG + chr2.4:70436483-70436502 None:intergenic 30.0%
AATAAAACAATGACACTCCT+TGG + chr2.4:70437420-70437439 None:intergenic 30.0%
AGCAAAAATTCAGCCAAAAT+TGG + chr2.4:70437626-70437645 None:intergenic 30.0%
ATCAAGATAGACATACAGAT+AGG - chr2.4:70437101-70437120 MS.gene01038:intron 30.0%
ATGATCAATATGGAGAAGAA+GGG - chr2.4:70436610-70436629 MS.gene01038:CDS 30.0%
CATAGTGTATTTACAAGAGA+TGG - chr2.4:70437830-70437849 MS.gene01038:CDS 30.0%
CCAAAAAGATGAAGATTTCA+AGG - chr2.4:70436976-70436995 MS.gene01038:intron 30.0%
GATTCATCCAAATTATGAAG+AGG - chr2.4:70437964-70437983 MS.gene01038:CDS 30.0%
GCAAAAATTCAGCCAAAATT+GGG + chr2.4:70437625-70437644 None:intergenic 30.0%
GGAATAACCTCTTCATAATT+TGG + chr2.4:70437974-70437993 None:intergenic 30.0%
GGATGAATCACATTGTTTAT+AGG + chr2.4:70437953-70437972 None:intergenic 30.0%
TAAATGTGATCTTCCATCAT+AGG - chr2.4:70437232-70437251 MS.gene01038:intron 30.0%
TATGATCAATATGGAGAAGA+AGG - chr2.4:70436609-70436628 MS.gene01038:CDS 30.0%
TATTTGAGGGATTCTTATCA+GGG + chr2.4:70436421-70436440 None:intergenic 30.0%
TCTCTTGTAAATACACTATG+TGG + chr2.4:70437830-70437849 None:intergenic 30.0%
TTAATATCAAGTTTCCAGCA+AGG - chr2.4:70438053-70438072 MS.gene01038:CDS 30.0%
TTATGAAGAGGTTATTCCAA+AGG - chr2.4:70437976-70437995 MS.gene01038:CDS 30.0%
TTATTTGAGGGATTCTTATC+AGG + chr2.4:70436422-70436441 None:intergenic 30.0%
TTTCTTCATTTGATGAAGGT+AGG - chr2.4:70436868-70436887 MS.gene01038:intron 30.0%
! AGACTACTACAAGATTTTGA+AGG - chr2.4:70436331-70436350 MS.gene01038:CDS 30.0%
! ATATCTTTGCAGAGTTTTTC+GGG - chr2.4:70436739-70436758 MS.gene01038:CDS 30.0%
! ATTTGGAATCAAGAAACTTC+TGG - chr2.4:70438093-70438112 MS.gene01038:CDS 30.0%
! CCTTGAAATCTTCATCTTTT+TGG + chr2.4:70436979-70436998 None:intergenic 30.0%
! GATATCTTTGCAGAGTTTTT+CGG - chr2.4:70436738-70436757 MS.gene01038:CDS 30.0%
!!! AATTGCAAGTTGTTTTTGTG+GGG - chr2.4:70437554-70437573 MS.gene01038:CDS 30.0%
!!! AATTTTGGCTGAATTTTTGC+TGG - chr2.4:70437625-70437644 MS.gene01038:CDS 30.0%
!!! TGGTTTTGGTTTTTTGATGA+AGG - chr2.4:70436557-70436576 MS.gene01038:CDS 30.0%
!!! TTTGAAGGATCTTTTGGAAT+AGG + chr2.4:70438010-70438029 None:intergenic 30.0%
AAAATCACATTCCCTGAGAA+AGG - chr2.4:70437749-70437768 MS.gene01038:CDS 35.0%
AAGATATCGTTTGCGTTTCT+TGG + chr2.4:70436726-70436745 None:intergenic 35.0%
ACATACAGATAGGAGATTGA+TGG - chr2.4:70437111-70437130 MS.gene01038:intron 35.0%
ATTGTTAAGATTTCCTCCAC+TGG + chr2.4:70437698-70437717 None:intergenic 35.0%
CCAAAAGATCCTTCAAAGAA+GGG - chr2.4:70438013-70438032 MS.gene01038:CDS 35.0%
CTAAGATTACCCTTCTTTGA+AGG + chr2.4:70438025-70438044 None:intergenic 35.0%
CTTCAAAGAAGGGTAATCTT+AGG - chr2.4:70438023-70438042 MS.gene01038:CDS 35.0%
GAAGTTAAGTCATCCTATGA+TGG + chr2.4:70437248-70437267 None:intergenic 35.0%
GAGAATTGATGTTGAACTTC+TGG + chr2.4:70437590-70437609 None:intergenic 35.0%
TAACAATTGAAGTCAAGCCA+GGG - chr2.4:70437711-70437730 MS.gene01038:CDS 35.0%
TACAAGAGATGGCAATGATT+TGG - chr2.4:70437841-70437860 MS.gene01038:CDS 35.0%
TCCAAAAGATCCTTCAAAGA+AGG - chr2.4:70438012-70438031 MS.gene01038:CDS 35.0%
TTAACAATTGAAGTCAAGCC+AGG - chr2.4:70437710-70437729 MS.gene01038:CDS 35.0%
TTGAACACAAAATCTACCGA+AGG + chr2.4:70437501-70437520 None:intergenic 35.0%
TTGACTTCTGAGCAGAAATT+TGG - chr2.4:70438076-70438095 MS.gene01038:CDS 35.0%
! AGATTTTGTGTTCAACGCAT+TGG - chr2.4:70437506-70437525 MS.gene01038:CDS 35.0%
! CAGTTGAATTTGGTTCAGTA+TGG - chr2.4:70437455-70437474 MS.gene01038:CDS 35.0%
! GAATTTGGTTCAGTATGGTA+GGG - chr2.4:70437460-70437479 MS.gene01038:CDS 35.0%
! TCAGCGTCTTTCTTATTTGA+GGG + chr2.4:70436434-70436453 None:intergenic 35.0%
! TGAATTTGGTTCAGTATGGT+AGG - chr2.4:70437459-70437478 MS.gene01038:CDS 35.0%
! TGAGTCATAAGTCCCAATTT+TGG - chr2.4:70437610-70437629 MS.gene01038:CDS 35.0%
AAACATTCCACCAAAGGATC+TGG + chr2.4:70436829-70436848 None:intergenic 40.0%
ACAATGACACTCCTTGGATT+AGG + chr2.4:70437414-70437433 None:intergenic 40.0%
ACATTCTTCCAAAGTGGAGA+TGG - chr2.4:70436678-70436697 MS.gene01038:CDS 40.0%
AGATCCTTTGGTGGAATGTT+TGG - chr2.4:70436828-70436847 MS.gene01038:intron 40.0%
AGATCTGCTGGTATCACATT+TGG + chr2.4:70437785-70437804 None:intergenic 40.0%
AGCCTTGAGGAACTCTATAA+AGG - chr2.4:70436948-70436967 MS.gene01038:intron 40.0%
ATCGAAAACTTGCCATGAAG+TGG - chr2.4:70436393-70436412 MS.gene01038:CDS 40.0%
ATGGAGAAGAAGGGCTAAAA+GGG - chr2.4:70436619-70436638 MS.gene01038:CDS 40.0%
CACAGAAGAAGAGTTGAAGA+AGG - chr2.4:70436367-70436386 MS.gene01038:CDS 40.0%
CTCAAATAAGAAAGACGCTG+AGG - chr2.4:70436433-70436452 MS.gene01038:CDS 40.0%
CTTCTTCAACTCTTCTTCTG+TGG + chr2.4:70436369-70436388 None:intergenic 40.0%
GAAGAGGTTATTCCAAAGGA+AGG - chr2.4:70437980-70437999 MS.gene01038:CDS 40.0%
GCCTTGAGGAACTCTATAAA+GGG - chr2.4:70436949-70436968 MS.gene01038:intron 40.0%
GGATTGAACCTAAACGTTGT+TGG + chr2.4:70436705-70436724 None:intergenic 40.0%
GTTGCTCATTTCCTTTCTCA+GGG + chr2.4:70437763-70437782 None:intergenic 40.0%
TATGGAGAAGAAGGGCTAAA+AGG - chr2.4:70436618-70436637 MS.gene01038:CDS 40.0%
TCCCTTTATAGAGTTCCTCA+AGG + chr2.4:70436953-70436972 None:intergenic 40.0%
! AGAGATATCTGAAGCCTATG+AGG - chr2.4:70436466-70436485 MS.gene01038:CDS 40.0%
! CTCAGCGTCTTTCTTATTTG+AGG + chr2.4:70436435-70436454 None:intergenic 40.0%
! TCACTTGCAGAAGCTTTAAC+AGG - chr2.4:70437881-70437900 MS.gene01038:intron 40.0%
!! CCCTTCTTTGAAGGATCTTT+TGG + chr2.4:70438016-70438035 None:intergenic 40.0%
!! TTGTGTTCAACGCATTGGTT+TGG - chr2.4:70437511-70437530 MS.gene01038:CDS 40.0%
ACCACCAAACATTCCACCAA+AGG + chr2.4:70436835-70436854 None:intergenic 45.0%
AGGGAAATTGCTGATGCAAG+TGG - chr2.4:70436996-70437015 MS.gene01038:intron 45.0%
CACAGACATCAGATGTGACA+TGG - chr2.4:70437293-70437312 MS.gene01038:intron 45.0%
GAGATGGACCAACAACGTTT+AGG - chr2.4:70436694-70436713 MS.gene01038:CDS 45.0%
GGAATAGGCATACCTTCCTT+TGG + chr2.4:70437995-70438014 None:intergenic 45.0%
GGGAAATTGCTGATGCAAGT+GGG - chr2.4:70436997-70437016 MS.gene01038:intron 45.0%
GGTTGCTCATTTCCTTTCTC+AGG + chr2.4:70437764-70437783 None:intergenic 45.0%
GTTCGTCTCACAACACTAGA+TGG - chr2.4:70437911-70437930 MS.gene01038:intron 45.0%
TAGAGTTCCTCAAGGCTACA+AGG + chr2.4:70436945-70436964 None:intergenic 45.0%
TCCTTTGGTGGAATGTTTGG+TGG - chr2.4:70436831-70436850 MS.gene01038:intron 45.0%
TCTCACAACACTAGATGGCA+GGG - chr2.4:70437916-70437935 MS.gene01038:intron 45.0%
TTGCAGGAAAACTATGCCAG+TGG - chr2.4:70437679-70437698 MS.gene01038:CDS 45.0%
TTGTTGGTCCATCTCCACTT+TGG + chr2.4:70436689-70436708 None:intergenic 45.0%
! TAGATTGCCTTGTAGCCTTG+AGG - chr2.4:70436935-70436954 MS.gene01038:intron 45.0%
! TTTGTACCCTTTTTCCACCC+TGG + chr2.4:70437731-70437750 None:intergenic 45.0%
AGTCAAGCCAGGGTGGAAAA+AGG - chr2.4:70437721-70437740 MS.gene01038:CDS 50.0%
ATTCCTCCCATTCCCCCAAA+CGG + chr2.4:70436774-70436793 None:intergenic 50.0%
CAATTGAAGTCAAGCCAGGG+TGG - chr2.4:70437714-70437733 MS.gene01038:CDS 50.0%
CACTGGACACACCTAATCCA+AGG - chr2.4:70437400-70437419 MS.gene01038:intron 50.0%
CAGGAAAACTATGCCAGTGG+AGG - chr2.4:70437682-70437701 MS.gene01038:CDS 50.0%
GACCAAGACAACGAGTCCTT+CGG - chr2.4:70437482-70437501 MS.gene01038:CDS 50.0%
GGATTAGGTGTGTCCAGTGT+CGG + chr2.4:70437399-70437418 None:intergenic 50.0%
GGGGCTACATTCTTCCAAAG+TGG - chr2.4:70436672-70436691 MS.gene01038:CDS 50.0%
GTCAAGCCAGGGTGGAAAAA+GGG - chr2.4:70437722-70437741 MS.gene01038:CDS 50.0%
GTCTCACAACACTAGATGGC+AGG - chr2.4:70437915-70437934 MS.gene01038:intron 50.0%
TACCGAAGGACTCGTTGTCT+TGG + chr2.4:70437487-70437506 None:intergenic 50.0%
TCGGGTATTCAAGCCCGTTT+GGG - chr2.4:70436757-70436776 MS.gene01038:intron 50.0%
TGCTCAGAAGTCAACCTTGC+TGG + chr2.4:70438070-70438089 None:intergenic 50.0%
TTCGGGTATTCAAGCCCGTT+TGG - chr2.4:70436756-70436775 MS.gene01038:intron 50.0%
TTTGGGGGAATGGGAGGAAT+GGG - chr2.4:70436774-70436793 MS.gene01038:intron 50.0%
!! CTTATCAGGGTGCCACTTCA+TGG + chr2.4:70436408-70436427 None:intergenic 50.0%
ACCTCAAGATGCTGCTGGTG+GGG - chr2.4:70436653-70436672 MS.gene01038:CDS 55.0%
AGAGGTGGAGCCAGATCCTT+TGG - chr2.4:70436816-70436835 MS.gene01038:intron 55.0%
AGGTAGGCCTTTGCGTCAAC+AGG - chr2.4:70436884-70436903 MS.gene01038:intron 55.0%
CACCTCAAGATGCTGCTGGT+GGG - chr2.4:70436652-70436671 MS.gene01038:CDS 55.0%
CGGGTATTCAAGCCCGTTTG+GGG - chr2.4:70436758-70436777 MS.gene01038:intron 55.0%
GCTGGTGGGAATGGAATGAG+AGG - chr2.4:70436798-70436817 MS.gene01038:intron 55.0%
GGGTATTCAAGCCCGTTTGG+GGG - chr2.4:70436759-70436778 MS.gene01038:intron 55.0%
GGTAGGCCTTTGCGTCAACA+GGG - chr2.4:70436885-70436904 MS.gene01038:intron 55.0%
GGTGGGAATGGAATGAGAGG+TGG - chr2.4:70436801-70436820 MS.gene01038:intron 55.0%
GTTTGGGGGAATGGGAGGAA+TGG - chr2.4:70436773-70436792 MS.gene01038:intron 55.0%
TCAAGCCCGTTTGGGGGAAT+GGG - chr2.4:70436765-70436784 MS.gene01038:intron 55.0%
TCAATAGCAGCTGCCTTGCG+AGG + chr2.4:70436912-70436931 None:intergenic 55.0%
TTCAAGCCCGTTTGGGGGAA+TGG - chr2.4:70436764-70436783 MS.gene01038:intron 55.0%
TTCCTCCCATTCCCCCAAAC+GGG + chr2.4:70436773-70436792 None:intergenic 55.0%
TTGGGGGAATGGGAGGAATG+GGG - chr2.4:70436775-70436794 MS.gene01038:intron 55.0%
CCACCACCTCAAGATGCTGC+TGG - chr2.4:70436648-70436667 MS.gene01038:CDS 60.0%
CCACCAGCAGCATCTTGAGG+TGG + chr2.4:70436654-70436673 None:intergenic 60.0%
CCACCTCAAGATGCTGCTGG+TGG - chr2.4:70436651-70436670 MS.gene01038:CDS 60.0%
CCAGCAGCATCTTGAGGTGG+TGG + chr2.4:70436651-70436670 None:intergenic 60.0%
CGAGGACCCTGTTGACGCAA+AGG + chr2.4:70436894-70436913 None:intergenic 60.0%
GGTGGAGCCAGATCCTTTGG+TGG - chr2.4:70436819-70436838 MS.gene01038:intron 60.0%
TGGGGGAATGGGAGGAATGG+GGG - chr2.4:70436776-70436795 MS.gene01038:intron 60.0%
AGCCCGTTTGGGGGAATGGG+AGG - chr2.4:70436768-70436787 MS.gene01038:intron 65.0%
ATGGGAGGAATGGGGGCTGG+TGG - chr2.4:70436783-70436802 MS.gene01038:intron 65.0%
GCCCCACCAGCAGCATCTTG+AGG + chr2.4:70436657-70436676 None:intergenic 65.0%
GCGTCAACAGGGTCCTCGCA+AGG - chr2.4:70436896-70436915 MS.gene01038:intron 65.0%
GGAATGGGAGGAATGGGGGC+TGG - chr2.4:70436780-70436799 MS.gene01038:intron 65.0%
! GGAATGGGGGCTGGTGGGAA+TGG - chr2.4:70436789-70436808 MS.gene01038:intron 65.0%
! TGGGAGGAATGGGGGCTGGT+GGG - chr2.4:70436784-70436803 MS.gene01038:intron 65.0%
CGCTGACGCGTGTCCGACAC+TGG - chr2.4:70437383-70437402 MS.gene01038:intron 70.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 70436323 70438123 70436323 ID=MS.gene01038
chr2.4 mRNA 70436323 70438123 70436323 ID=MS.gene01038.t1;Parent=MS.gene01038
chr2.4 exon 70437959 70438123 70437959 ID=MS.gene01038.t1.exon1;Parent=MS.gene01038.t1
chr2.4 CDS 70437959 70438123 70437959 ID=cds.MS.gene01038.t1;Parent=MS.gene01038.t1
chr2.4 exon 70437428 70437867 70437428 ID=MS.gene01038.t1.exon2;Parent=MS.gene01038.t1
chr2.4 CDS 70437428 70437867 70437428 ID=cds.MS.gene01038.t1;Parent=MS.gene01038.t1
chr2.4 exon 70436323 70436761 70436323 ID=MS.gene01038.t1.exon3;Parent=MS.gene01038.t1
chr2.4 CDS 70436323 70436761 70436323 ID=cds.MS.gene01038.t1;Parent=MS.gene01038.t1
Gene Sequence

>MS.gene01038

ATGGGGGTAGACTACTACAAGATTTTGAAGGTTGACAAGAATGCCACAGAAGAAGAGTTGAAGAAGGCTTATCGAAAACTTGCCATGAAGTGGCACCCTGATAAGAATCCCTCAAATAAGAAAGACGCTGAGGCCAAATTCAAAGAGATATCTGAAGCCTATGAGGTAACATTACAGTTTCTTTAATCTTCTGTTACTAAGTGCCAATTCTTCATAGAAATTCTGAATATTAACTGGTTTTGGTTTTTTGATGAAGGTTCTAAGTGATCCACAAAAGAAAGCAATTTATGATCAATATGGAGAAGAAGGGCTAAAAGGGCAAGTACCACCACCTCAAGATGCTGCTGGTGGGGCTACATTCTTCCAAAGTGGAGATGGACCAACAACGTTTAGGTTCAATCCAAGAAACGCAAACGATATCTTTGCAGAGTTTTTCGGGTATTCAAGCCCGTTTGGGGGAATGGGAGGAATGGGGGCTGGTGGGAATGGAATGAGAGGTGGAGCCAGATCCTTTGGTGGAATGTTTGGTGGTGATGATCATATGTTTTCTTCATTTGATGAAGGTAGGCCTTTGCGTCAACAGGGTCCTCGCAAGGCAGCTGCTATTGAAAATAGATTGCCTTGTAGCCTTGAGGAACTCTATAAAGGGACTACCAAAAAGATGAAGATTTCAAGGGAAATTGCTGATGCAAGTGGGTAAGTATTCTTTGCATAACTTTCTATTGTTACATCACTATATGTTGGTTGTTAGATTCCTCAAAATATGTTGGATCTAATTATCAAGATAGACATACAGATAGGAGATTGATGGATGAATCCTGCTTTTTGAAATAATTTAGAACACGAGTTATATCAATTACTTAATTGTATTTATGTATATCATAAACTTAATTGTATTTATGTATATCATAAATGTGATCTTCCATCATAGGATGACTTAACTTCAATAATTTAGATGATGAAGCACGGACACAGACATCAGATGTGACATGGACACTAATACACTGACATCAATAATAATTTAAAAAACTGGCATTTAGCGTAATCATAGTTGTCGTGTCGCTGACGCGTGTCCGACACTGGACACACCTAATCCAAGGAGTGTCATTGTTTTATTTGATCAAAAGTTGAACAGTTGAATTTGGTTCAGTATGGTAGGGACCAAGACAACGAGTCCTTCGGTAGATTTTGTGTTCAACGCATTGGTTTGGTTGATTCAGTCCGATTTTTAAATTGCAAGTTGTTTTTGTGGGGTTGTATTATACCAGAAGTTCAACATCAATTCTCTGAGTCATAAGTCCCAATTTTGGCTGAATTTTTGCTGGTCTAATTAAATTTGCCTAATTATTTATCTCTTTGCAGGAAAACTATGCCAGTGGAGGAAATCTTAACAATTGAAGTCAAGCCAGGGTGGAAAAAGGGTACAAAAATCACATTCCCTGAGAAAGGAAATGAGCAACCAAATGTGATACCAGCAGATCTTATATTTGTTATTGATGAGAAGCCACATAGTGTATTTACAAGAGATGGCAATGATTTGGTTGCAACACAGAAGATATCACTTGCAGAAGCTTTAACAGGCTACACTGTTCGTCTCACAACACTAGATGGCAGGGTTCTGAATGTTCCTATAAACAATGTGATTCATCCAAATTATGAAGAGGTTATTCCAAAGGAAGGTATGCCTATTCCAAAAGATCCTTCAAAGAAGGGTAATCTTAGGATCAAATTTAATATCAAGTTTCCAGCAAGGTTGACTTCTGAGCAGAAATTTGGAATCAAGAAACTTCTGGCACCGTAG
Protein sequence

>MS.gene01038.t1

MGVDYYKILKVDKNATEEELKKAYRKLAMKWHPDKNPSNKKDAEAKFKEISEAYEVLSDPQKKAIYDQYGEEGLKGQVPPPQDAAGGATFFQSGDGPTTFRFNPRNANDIFAEFFGYSSPFGGMGGMGAGGNGMRGGARSFGGMFGGDDHMFSSFDEGRPLRQQGPRKAAAIENRLPCSLEELYKGTTKKMKISREIADASGKTMPVEEILTIEVKPGWKKGTKITFPEKGNEQPNVIPADLIFVIDEKPHSVFTRDGNDLVATQKISLAEALTGYTVRLTTLDGRVLNVPINNVIHPNYEEVIPKEGMPIPKDPSKKGNLRIKFNIKFPARLTSEQKFGIKKLLAP