AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene024514


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene024514.t1 MTR_7g116340 98.837 172 2 0 1 172 1 172 2.32e-126 352
MS.gene024514.t1 MTR_4g075290 76.744 172 40 0 1 172 1 172 5.84e-100 285
MS.gene024514.t1 MTR_3g117750 75.595 168 41 0 5 172 13 180 4.26e-95 274
MS.gene024514.t1 MTR_3g463900 69.461 167 51 0 5 171 35 201 3.80e-84 246
MS.gene024514.t1 MTR_3g070500 67.857 168 54 0 5 172 65 232 6.34e-82 242
MS.gene024514.t1 MTR_7g095750 64.912 171 59 1 3 172 6 176 1.64e-76 240
MS.gene024514.t1 MTR_8g079690 64.000 175 59 3 1 172 1 174 6.08e-72 221
MS.gene024514.t1 MTR_1g085560 68.493 146 39 1 5 150 86 224 1.89e-69 211
MS.gene024514.t1 MTR_1g117010 59.649 171 58 3 5 171 78 241 2.88e-67 205
MS.gene024514.t1 MTR_1g117010 59.649 171 58 3 5 171 78 241 2.88e-67 205
MS.gene024514.t1 MTR_8g040180 59.064 171 66 3 3 171 17 185 6.62e-66 199
MS.gene024514.t1 MTR_7g110980 65.753 146 43 1 5 150 97 235 8.45e-66 202
MS.gene024514.t1 MTR_4g086760 59.538 173 68 2 1 171 1 173 6.29e-65 201
MS.gene024514.t1 MTR_4g086760 59.538 173 68 2 1 171 1 173 6.93e-65 202
MS.gene024514.t1 MTR_4g086760 59.538 173 68 2 1 171 1 173 7.76e-65 202
MS.gene024514.t1 MTR_3g070500 74.194 124 32 0 5 128 65 188 1.17e-64 196
MS.gene024514.t1 MTR_4g073650 54.762 168 75 1 5 171 47 214 9.66e-63 193
MS.gene024514.t1 MTR_3g107660 53.254 169 78 1 3 170 9 177 1.91e-51 176
MS.gene024514.t1 MTR_3g070500 73.737 99 26 0 5 103 65 163 4.41e-49 156
MS.gene024514.t1 MTR_3g070500 73.469 98 26 0 5 102 65 162 6.43e-49 156
MS.gene024514.t1 MTR_1g011980 51.479 169 80 2 3 170 6 173 1.17e-47 165
MS.gene024514.t1 MTR_2g085075 51.034 145 59 3 11 154 464 597 6.73e-37 134
MS.gene024514.t1 MTR_7g105220 49.286 140 59 4 18 156 19 147 7.99e-37 125
MS.gene024514.t1 MTR_8g036015 52.206 136 53 3 18 152 10 134 1.85e-36 124
MS.gene024514.t1 MTR_5g015500 49.333 150 63 5 18 165 314 452 2.41e-32 121
MS.gene024514.t1 MTR_5g015500 49.333 150 63 5 18 165 354 492 3.64e-32 120
MS.gene024514.t1 MTR_3g085705 60.638 94 32 3 57 150 27 115 1.93e-31 110
MS.gene024514.t1 MTR_2g023120 46.825 126 50 5 18 139 22 134 2.46e-21 90.1
MS.gene024514.t1 MTR_6g084140 34.591 159 83 5 18 166 27 174 9.15e-21 88.6
MS.gene024514.t1 MTR_6g084140 34.591 159 83 5 18 166 10 157 1.04e-20 88.6
MS.gene024514.t1 MTR_8g442420 33.962 159 84 5 18 166 10 157 1.99e-19 84.7
MS.gene024514.t1 MTR_4g111720 25.907 193 98 4 5 160 100 284 2.07e-12 64.3
MS.gene024514.t1 MTR_1g035360 30.556 144 83 6 12 154 82 209 3.07e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene024514.t1 AT3G56070 83.626 171 28 0 1 171 1 171 1.46e-107 305
MS.gene024514.t1 AT3G56070 83.626 171 28 0 1 171 1 171 1.46e-107 305
MS.gene024514.t1 AT2G16600 78.947 171 36 0 2 172 3 173 1.96e-100 286
MS.gene024514.t1 AT2G21130 77.193 171 39 0 2 172 3 173 5.94e-99 283
MS.gene024514.t1 AT4G38740 74.419 172 44 0 1 172 1 172 9.18e-96 275
MS.gene024514.t1 AT4G34870 75.000 172 43 0 1 172 1 172 8.59e-95 272
MS.gene024514.t1 AT2G29960 70.060 167 50 0 5 171 33 199 1.01e-84 248
MS.gene024514.t1 AT2G29960 70.060 167 50 0 5 171 33 199 1.01e-84 248
MS.gene024514.t1 AT5G58710 69.461 167 51 0 5 171 36 202 1.87e-81 239
MS.gene024514.t1 AT2G16600 67.836 171 33 1 2 172 3 151 3.69e-78 229
MS.gene024514.t1 AT3G55920 64.881 168 59 0 5 172 60 227 8.99e-78 231
MS.gene024514.t1 AT3G63400 65.497 171 58 1 3 172 6 176 7.27e-76 232
MS.gene024514.t1 AT2G29960 65.269 167 48 1 5 171 33 189 2.97e-75 223
MS.gene024514.t1 AT3G63400 65.497 171 58 1 3 172 6 176 4.57e-74 232
MS.gene024514.t1 AT3G63400 65.497 171 58 1 3 172 6 176 4.57e-74 232
MS.gene024514.t1 AT3G63400 65.497 171 58 1 3 172 6 176 4.57e-74 232
MS.gene024514.t1 AT5G13120 61.988 171 54 3 5 171 87 250 6.13e-72 218
MS.gene024514.t1 AT5G13120 61.988 171 54 3 5 171 91 254 6.43e-72 218
MS.gene024514.t1 AT3G62030 69.863 146 37 1 5 150 95 233 8.71e-70 212
MS.gene024514.t1 AT3G62030 69.863 146 37 1 5 150 96 234 9.08e-70 212
MS.gene024514.t1 AT3G62030 69.863 146 37 1 5 150 149 287 4.99e-69 212
MS.gene024514.t1 AT2G15790 61.272 173 65 2 1 171 1 173 1.85e-67 209
MS.gene024514.t1 AT2G38730 59.649 171 65 3 3 171 31 199 2.66e-65 199
MS.gene024514.t1 AT4G34960 53.571 168 77 1 5 171 48 215 1.58e-61 190
MS.gene024514.t1 AT3G22920 55.114 176 67 6 1 172 1 168 3.91e-54 171
MS.gene024514.t1 AT4G34960 55.645 124 55 0 5 128 48 171 4.81e-48 154
MS.gene024514.t1 AT4G32420 49.704 169 84 1 3 170 6 174 5.75e-48 166
MS.gene024514.t1 AT4G32420 49.704 169 84 1 3 170 6 174 5.75e-48 166
MS.gene024514.t1 AT4G32420 49.704 169 84 1 3 170 6 174 5.75e-48 166
MS.gene024514.t1 AT4G32420 49.704 169 84 1 3 170 6 174 5.75e-48 166
MS.gene024514.t1 AT4G32420 50.318 157 77 1 3 158 6 162 9.87e-45 157
MS.gene024514.t1 AT2G36130 50.000 140 58 4 18 156 19 147 1.12e-37 127
MS.gene024514.t1 AT3G44600 49.045 157 67 4 11 165 478 623 2.37e-37 135
MS.gene024514.t1 AT1G01940 52.206 136 53 3 18 152 10 134 3.60e-37 126
MS.gene024514.t1 AT5G67530 48.667 150 64 5 18 165 353 491 3.17e-32 121
MS.gene024514.t1 AT4G33060 47.200 125 53 4 18 140 22 135 1.48e-27 107
MS.gene024514.t1 AT1G53720 39.130 138 64 5 18 146 10 136 1.66e-20 87.0
MS.gene024514.t1 AT1G53720 39.130 138 64 5 18 146 10 136 2.30e-20 87.4

Find 46 sgRNAs with CRISPR-Local

Find 54 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATGGAAAGCATGTTGTATT+TGG 0.221121 7.2:+1689458 MS.gene024514:CDS
ATTCTTTCCATGGCAAATTC+TGG 0.225996 7.2:+1689380 MS.gene024514:CDS
ACTGGAGGAGAGTCTATATA+TGG 0.292463 7.2:+1689308 MS.gene024514:CDS
GAGAAGGTTGGTTCACAAAA+TGG 0.318577 7.2:+1689518 MS.gene024514:CDS
GGTAAACCTTTACACTATAA+AGG 0.338627 7.2:+1689218 MS.gene024514:CDS
CCTTTACACTATAAAGGTTC+TGG 0.340990 7.2:+1689224 MS.gene024514:CDS
AGAATATCAAAGAAAACCTT+TGG 0.366302 7.2:-1689080 None:intergenic
TCAAAGACTCAATGGCTTGA+TGG 0.389006 7.2:+1689440 MS.gene024514:CDS
ATCAAGCCATTGAGTCTTTG+AGG 0.392504 7.2:-1689438 None:intergenic
TGCACAGGAGAAAGGGGTAT+TGG 0.393886 7.2:+1689188 MS.gene024514:CDS
GCACAGGAGAAAGGGGTATT+GGG 0.401126 7.2:+1689189 MS.gene024514:CDS
AACTGAGAACCATTAGTATT+GGG 0.402674 7.2:-1689404 None:intergenic
AATTTCAAGCTGAAGCATAC+TGG 0.411931 7.2:+1689350 MS.gene024514:CDS
CGAAGGATACGATGTAGTTC+AGG 0.437134 7.2:+1689490 MS.gene024514:CDS
CCAGAACCTTTATAGTGTAA+AGG 0.437419 7.2:-1689224 None:intergenic
AAACTGAGAACCATTAGTAT+TGG 0.443961 7.2:-1689405 None:intergenic
TTTACCAAAGGGAATGGTAC+TGG 0.450711 7.2:+1689290 MS.gene024514:CDS
TTTGTACCTCAAAGACTCAA+TGG 0.453659 7.2:+1689432 MS.gene024514:CDS
AATTCTGGACCCAATACTAA+TGG 0.454102 7.2:+1689395 MS.gene024514:CDS
GGCACTCTGCACAGGAGAAA+GGG 0.455813 7.2:+1689181 MS.gene024514:CDS
TCCTCCAGTACCATTCCCTT+TGG 0.468424 7.2:-1689294 None:intergenic
GGGCACTCTGCACAGGAGAA+AGG 0.484529 7.2:+1689180 MS.gene024514:CDS
GCATGTTGTATTTGGAAAAG+TGG 0.496708 7.2:+1689466 MS.gene024514:CDS
AAATGGTAGGACATCAGAAC+AGG 0.503914 7.2:+1689535 MS.gene024514:CDS
ATTCCAGAGTTTATGTGTCA+AGG 0.523820 7.2:+1689260 MS.gene024514:CDS
TTGTGAACCAACCTTCTCCA+TGG 0.533815 7.2:-1689513 None:intergenic
TGTAACCATGTCAAATCCAA+AGG 0.535644 7.2:+1689064 None:intergenic
CTAATTGGAAGAATGAAAGC+TGG 0.537372 7.2:+1689101 MS.gene024514:CDS
TGGACCTGGTATTCTTTCCA+TGG 0.538902 7.2:+1689370 MS.gene024514:CDS
CCTCCTTGACACATAAACTC+TGG 0.550153 7.2:-1689263 None:intergenic
CTGGAGGAGAGTCTATATAT+GGG 0.556827 7.2:+1689309 MS.gene024514:CDS
AAGCTGAAGCATACTGGACC+TGG 0.564886 7.2:+1689356 MS.gene024514:CDS
ATACGATGTAGTTCAGGCCA+TGG 0.568695 7.2:+1689496 MS.gene024514:CDS
TGTAGTTCAGGCCATGGAGA+AGG 0.577243 7.2:+1689502 MS.gene024514:CDS
GAAAGCTGGAAAGATAGTAA+TGG 0.582740 7.2:+1689115 MS.gene024514:CDS
TTTGCCATGGAAAGAATACC+AGG 0.593087 7.2:-1689374 None:intergenic
GTTCAGGCCATGGAGAAGGT+TGG 0.594001 7.2:+1689506 MS.gene024514:CDS
GAAAACCTTTGGATTTGACA+TGG 0.607760 7.2:-1689069 None:intergenic
GTATTTGGAAAAGTGGTCGA+AGG 0.607981 7.2:+1689473 MS.gene024514:CDS
ATTGGGTCCAGAATTTGCCA+TGG 0.609063 7.2:-1689387 None:intergenic
CAGGTTGTCATCGAAGATTG+TGG 0.618674 7.2:+1689554 MS.gene024514:CDS
AGGTTGGTTCACAAAATGGT+AGG 0.628168 7.2:+1689522 MS.gene024514:CDS
GCACTCTGCACAGGAGAAAG+GGG 0.634116 7.2:+1689182 MS.gene024514:CDS
ACCAAAGGGAATGGTACTGG+AGG 0.652379 7.2:+1689293 MS.gene024514:CDS
GAAAGGGGTATTGGGCAATG+CGG 0.661531 7.2:+1689197 MS.gene024514:CDS
CCAGAGTTTATGTGTCAAGG+AGG 0.669137 7.2:+1689263 MS.gene024514:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAACAGCTGAAAATTTTA+GGG + chr7.2:1689160-1689179 MS.gene024514:CDS 20.0%
!!! GTTTTCTTTGATATTCTAAT+TGG + chr7.2:1689086-1689105 MS.gene024514:CDS 20.0%
!!! TAAAATTTTCAGCTGTTTTT+GGG - chr7.2:1689160-1689179 None:intergenic 20.0%
! AGAATATCAAAGAAAACCTT+TGG - chr7.2:1689083-1689102 None:intergenic 25.0%
!! CAAAAACAGCTGAAAATTTT+AGG + chr7.2:1689159-1689178 MS.gene024514:CDS 25.0%
!!! AAAATTTTCAGCTGTTTTTG+GGG - chr7.2:1689159-1689178 None:intergenic 25.0%
!!! CTAAAATTTTCAGCTGTTTT+TGG - chr7.2:1689161-1689180 None:intergenic 25.0%
AAACTGAGAACCATTAGTAT+TGG - chr7.2:1689408-1689427 None:intergenic 30.0%
AACTGAGAACCATTAGTATT+GGG - chr7.2:1689407-1689426 None:intergenic 30.0%
GGTAAACCTTTACACTATAA+AGG + chr7.2:1689218-1689237 MS.gene024514:CDS 30.0%
AATTCTGGACCCAATACTAA+TGG + chr7.2:1689395-1689414 MS.gene024514:CDS 35.0%
AATTTCAAGCTGAAGCATAC+TGG + chr7.2:1689350-1689369 MS.gene024514:CDS 35.0%
ATTCCAGAGTTTATGTGTCA+AGG + chr7.2:1689260-1689279 MS.gene024514:CDS 35.0%
ATTCTTTCCATGGCAAATTC+TGG + chr7.2:1689380-1689399 MS.gene024514:CDS 35.0%
CCAGAACCTTTATAGTGTAA+AGG - chr7.2:1689227-1689246 None:intergenic 35.0%
CCTTTACACTATAAAGGTTC+TGG + chr7.2:1689224-1689243 MS.gene024514:CDS 35.0%
CTAATTGGAAGAATGAAAGC+TGG + chr7.2:1689101-1689120 MS.gene024514:CDS 35.0%
GAAAGCTGGAAAGATAGTAA+TGG + chr7.2:1689115-1689134 MS.gene024514:CDS 35.0%
GCATGTTGTATTTGGAAAAG+TGG + chr7.2:1689466-1689485 MS.gene024514:CDS 35.0%
TTTGTACCTCAAAGACTCAA+TGG + chr7.2:1689432-1689451 MS.gene024514:CDS 35.0%
! AAGGAGGAGATTTTACCAAA+GGG + chr7.2:1689279-1689298 MS.gene024514:CDS 35.0%
! GATGGAAAGCATGTTGTATT+TGG + chr7.2:1689458-1689477 MS.gene024514:CDS 35.0%
AAATGGTAGGACATCAGAAC+AGG + chr7.2:1689535-1689554 MS.gene024514:CDS 40.0%
ACTGGAGGAGAGTCTATATA+TGG + chr7.2:1689308-1689327 MS.gene024514:CDS 40.0%
ATCAAGCCATTGAGTCTTTG+AGG - chr7.2:1689441-1689460 None:intergenic 40.0%
CTGGAGGAGAGTCTATATAT+GGG + chr7.2:1689309-1689328 MS.gene024514:CDS 40.0%
GTATTTGGAAAAGTGGTCGA+AGG + chr7.2:1689473-1689492 MS.gene024514:CDS 40.0%
TCAAAGACTCAATGGCTTGA+TGG + chr7.2:1689440-1689459 MS.gene024514:CDS 40.0%
TTTACCAAAGGGAATGGTAC+TGG + chr7.2:1689290-1689309 MS.gene024514:CDS 40.0%
TTTGCCATGGAAAGAATACC+AGG - chr7.2:1689377-1689396 None:intergenic 40.0%
! AGGTTGGTTCACAAAATGGT+AGG + chr7.2:1689522-1689541 MS.gene024514:CDS 40.0%
! CAAGGAGGAGATTTTACCAA+AGG + chr7.2:1689278-1689297 MS.gene024514:CDS 40.0%
! GAGAAGGTTGGTTCACAAAA+TGG + chr7.2:1689518-1689537 MS.gene024514:CDS 40.0%
! GGAGATTTTACCAAAGGGAA+TGG + chr7.2:1689284-1689303 MS.gene024514:CDS 40.0%
ATACGATGTAGTTCAGGCCA+TGG + chr7.2:1689496-1689515 MS.gene024514:CDS 45.0%
ATTGGGTCCAGAATTTGCCA+TGG - chr7.2:1689390-1689409 None:intergenic 45.0%
CAGGTTGTCATCGAAGATTG+TGG + chr7.2:1689554-1689573 MS.gene024514:CDS 45.0%
CCAGAGTTTATGTGTCAAGG+AGG + chr7.2:1689263-1689282 MS.gene024514:CDS 45.0%
CCTCCTTGACACATAAACTC+TGG - chr7.2:1689266-1689285 None:intergenic 45.0%
CGAAGGATACGATGTAGTTC+AGG + chr7.2:1689490-1689509 MS.gene024514:CDS 45.0%
TGGACCTGGTATTCTTTCCA+TGG + chr7.2:1689370-1689389 MS.gene024514:CDS 45.0%
TTGTGAACCAACCTTCTCCA+TGG - chr7.2:1689516-1689535 None:intergenic 45.0%
!!! AATTTTAGGGCACTCTGCAC+AGG + chr7.2:1689173-1689192 MS.gene024514:CDS 45.0%
AAGCTGAAGCATACTGGACC+TGG + chr7.2:1689356-1689375 MS.gene024514:CDS 50.0%
TCCTCCAGTACCATTCCCTT+TGG - chr7.2:1689297-1689316 None:intergenic 50.0%
TGTAGTTCAGGCCATGGAGA+AGG + chr7.2:1689502-1689521 MS.gene024514:CDS 50.0%
! ACCAAAGGGAATGGTACTGG+AGG + chr7.2:1689293-1689312 MS.gene024514:CDS 50.0%
! TGCACAGGAGAAAGGGGTAT+TGG + chr7.2:1689188-1689207 MS.gene024514:CDS 50.0%
!! GAAAGGGGTATTGGGCAATG+CGG + chr7.2:1689197-1689216 MS.gene024514:CDS 50.0%
!! GCACAGGAGAAAGGGGTATT+GGG + chr7.2:1689189-1689208 MS.gene024514:CDS 50.0%
GCACTCTGCACAGGAGAAAG+GGG + chr7.2:1689182-1689201 MS.gene024514:CDS 55.0%
GGCACTCTGCACAGGAGAAA+GGG + chr7.2:1689181-1689200 MS.gene024514:CDS 55.0%
GTTCAGGCCATGGAGAAGGT+TGG + chr7.2:1689506-1689525 MS.gene024514:CDS 55.0%
GGGCACTCTGCACAGGAGAA+AGG + chr7.2:1689180-1689199 MS.gene024514:CDS 60.0%


Chromosome Type Strat End Strand Name
chr7.2 gene 1689071 1689589 1689071 ID=MS.gene024514
chr7.2 mRNA 1689071 1689589 1689071 ID=MS.gene024514.t1;Parent=MS.gene024514
chr7.2 exon 1689071 1689589 1689071 ID=MS.gene024514.t1.exon1;Parent=MS.gene024514.t1
chr7.2 CDS 1689071 1689589 1689071 ID=cds.MS.gene024514.t1;Parent=MS.gene024514.t1
Gene Sequence

>MS.gene024514

ATGTCAAATCCAAAGGTTTTCTTTGATATTCTAATTGGAAGAATGAAAGCTGGAAAGATAGTAATGGAGCTGTTTGCTGATGCTACCCCAAAAACAGCTGAAAATTTTAGGGCACTCTGCACAGGAGAAAGGGGTATTGGGCAATGCGGTAAACCTTTACACTATAAAGGTTCTGGTTTTCATCGTATTATTCCAGAGTTTATGTGTCAAGGAGGAGATTTTACCAAAGGGAATGGTACTGGAGGAGAGTCTATATATGGGTCTAAATTCGCTGACGAGAATTTCAAGCTGAAGCATACTGGACCTGGTATTCTTTCCATGGCAAATTCTGGACCCAATACTAATGGTTCTCAGTTTTTCATTTGTACCTCAAAGACTCAATGGCTTGATGGAAAGCATGTTGTATTTGGAAAAGTGGTCGAAGGATACGATGTAGTTCAGGCCATGGAGAAGGTTGGTTCACAAAATGGTAGGACATCAGAACAGGTTGTCATCGAAGATTGTGGACAAATTAGTTGA

Protein sequence

>MS.gene024514.t1

MSNPKVFFDILIGRMKAGKIVMELFADATPKTAENFRALCTGERGIGQCGKPLHYKGSGFHRIIPEFMCQGGDFTKGNGTGGESIYGSKFADENFKLKHTGPGILSMANSGPNTNGSQFFICTSKTQWLDGKHVVFGKVVEGYDVVQAMEKVGSQNGRTSEQVVIEDCGQIS