Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23546.t1 | XP_013445004.1 | 98.8 | 160 | 2 | 0 | 1 | 160 | 1 | 160 | 1.40E-88 | 335.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23546.t1 | Q9LPC7 | 90.6 | 160 | 15 | 0 | 1 | 160 | 1 | 160 | 7.1e-86 | 317.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23546.t1 | G7ZW00 | 98.8 | 160 | 2 | 0 | 1 | 160 | 1 | 160 | 9.8e-89 | 335.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050750 | MS.gene23546 | 0.807335 | 5.31E-50 | -1.69E-46 |
| MS.gene052012 | MS.gene23546 | 0.807088 | 6.00E-50 | -1.69E-46 |
| MS.gene053008 | MS.gene23546 | 0.814514 | 1.48E-51 | -1.69E-46 |
| MS.gene053080 | MS.gene23546 | 0.809261 | 2.07E-50 | -1.69E-46 |
| MS.gene053166 | MS.gene23546 | 0.822986 | 1.77E-53 | -1.69E-46 |
| MS.gene053953 | MS.gene23546 | 0.817078 | 3.97E-52 | -1.69E-46 |
| MS.gene054472 | MS.gene23546 | 0.802858 | 4.59E-49 | -1.69E-46 |
| MS.gene054559 | MS.gene23546 | 0.814776 | 1.30E-51 | -1.69E-46 |
| MS.gene054657 | MS.gene23546 | 0.808417 | 3.13E-50 | -1.69E-46 |
| MS.gene054658 | MS.gene23546 | 0.800168 | 1.64E-48 | -1.69E-46 |
| MS.gene055623 | MS.gene23546 | 0.801637 | 8.19E-49 | -1.69E-46 |
| MS.gene055818 | MS.gene23546 | 0.812778 | 3.57E-51 | -1.69E-46 |
| MS.gene056366 | MS.gene23546 | 0.808706 | 2.72E-50 | -1.69E-46 |
| MS.gene056498 | MS.gene23546 | 0.817383 | 3.39E-52 | -1.69E-46 |
| MS.gene056506 | MS.gene23546 | 0.823771 | 1.16E-53 | -1.69E-46 |
| MS.gene057026 | MS.gene23546 | 0.81704 | 4.05E-52 | -1.69E-46 |
| MS.gene057060 | MS.gene23546 | 0.816496 | 5.37E-52 | -1.69E-46 |
| MS.gene057414 | MS.gene23546 | 0.805494 | 1.30E-49 | -1.69E-46 |
| MS.gene05954 | MS.gene23546 | 0.805528 | 1.28E-49 | -1.69E-46 |
| MS.gene059569 | MS.gene23546 | 0.83694 | 6.97E-57 | -1.69E-46 |
| MS.gene060322 | MS.gene23546 | 0.808554 | 2.93E-50 | -1.69E-46 |
| MS.gene060572 | MS.gene23546 | 0.854144 | 1.50E-61 | -1.69E-46 |
| MS.gene060803 | MS.gene23546 | 0.81263 | 3.85E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene23546 | MS.gene04515 | PPI |
| MS.gene052266 | MS.gene23546 | PPI |
| MS.gene23546 | MS.gene04526 | PPI |
| MS.gene050574 | MS.gene23546 | PPI |
| MS.gene39668 | MS.gene23546 | PPI |
| MS.gene003412 | MS.gene23546 | PPI |
| MS.gene61767 | MS.gene23546 | PPI |
| MS.gene28963 | MS.gene23546 | PPI |
| MS.gene68752 | MS.gene23546 | PPI |
| MS.gene88451 | MS.gene23546 | PPI |
| MS.gene050281 | MS.gene23546 | PPI |
| MS.gene22872 | MS.gene23546 | PPI |
| MS.gene23546 | MS.gene024514 | PPI |
| MS.gene063104 | MS.gene23546 | PPI |
| MS.gene28703 | MS.gene23546 | PPI |
| MS.gene063787 | MS.gene23546 | PPI |
| MS.gene23546 | MS.gene007179 | PPI |
| MS.gene025980 | MS.gene23546 | PPI |
| MS.gene68153 | MS.gene23546 | PPI |
| MS.gene014216 | MS.gene23546 | PPI |
| MS.gene052269 | MS.gene23546 | PPI |
| MS.gene048458 | MS.gene23546 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23546.t1 | MTR_8g036015 | 98.750 | 160 | 2 | 0 | 1 | 160 | 1 | 160 | 3.14e-118 | 330 |
| MS.gene23546.t1 | MTR_5g015500 | 56.129 | 155 | 68 | 0 | 3 | 157 | 307 | 461 | 7.59e-58 | 189 |
| MS.gene23546.t1 | MTR_5g015500 | 56.129 | 155 | 68 | 0 | 3 | 157 | 347 | 501 | 2.02e-57 | 189 |
| MS.gene23546.t1 | MTR_7g105220 | 50.331 | 151 | 75 | 0 | 3 | 153 | 12 | 162 | 3.36e-54 | 169 |
| MS.gene23546.t1 | MTR_2g085075 | 50.000 | 152 | 76 | 0 | 2 | 153 | 463 | 614 | 1.35e-50 | 171 |
| MS.gene23546.t1 | MTR_8g079690 | 49.664 | 149 | 62 | 3 | 10 | 146 | 18 | 165 | 1.42e-40 | 139 |
| MS.gene23546.t1 | MTR_4g086760 | 49.669 | 151 | 63 | 4 | 10 | 148 | 18 | 167 | 6.83e-40 | 137 |
| MS.gene23546.t1 | MTR_4g086760 | 49.669 | 151 | 63 | 4 | 10 | 148 | 18 | 167 | 1.10e-39 | 137 |
| MS.gene23546.t1 | MTR_4g086760 | 49.669 | 151 | 63 | 4 | 10 | 148 | 18 | 167 | 1.11e-39 | 137 |
| MS.gene23546.t1 | MTR_7g095750 | 51.678 | 149 | 58 | 5 | 12 | 148 | 23 | 169 | 3.40e-37 | 134 |
| MS.gene23546.t1 | MTR_1g117010 | 46.897 | 145 | 72 | 4 | 9 | 149 | 93 | 236 | 1.73e-36 | 126 |
| MS.gene23546.t1 | MTR_1g117010 | 46.897 | 145 | 72 | 4 | 9 | 149 | 93 | 236 | 1.73e-36 | 126 |
| MS.gene23546.t1 | MTR_7g116340 | 51.471 | 136 | 54 | 3 | 10 | 134 | 18 | 152 | 2.14e-36 | 124 |
| MS.gene23546.t1 | MTR_7g110980 | 50.345 | 145 | 67 | 4 | 8 | 148 | 108 | 251 | 1.85e-35 | 124 |
| MS.gene23546.t1 | MTR_4g075290 | 46.203 | 158 | 67 | 6 | 10 | 152 | 18 | 172 | 5.47e-34 | 117 |
| MS.gene23546.t1 | MTR_1g085560 | 50.345 | 145 | 64 | 5 | 10 | 148 | 99 | 241 | 1.35e-33 | 119 |
| MS.gene23546.t1 | MTR_6g084140 | 38.554 | 166 | 94 | 2 | 1 | 158 | 1 | 166 | 7.91e-33 | 122 |
| MS.gene23546.t1 | MTR_6g084140 | 38.554 | 166 | 94 | 2 | 1 | 158 | 18 | 183 | 8.10e-33 | 122 |
| MS.gene23546.t1 | MTR_8g040180 | 48.252 | 143 | 63 | 4 | 10 | 142 | 32 | 173 | 9.01e-33 | 115 |
| MS.gene23546.t1 | MTR_3g117750 | 47.222 | 144 | 60 | 5 | 9 | 139 | 25 | 165 | 1.90e-32 | 114 |
| MS.gene23546.t1 | MTR_2g023120 | 45.283 | 159 | 83 | 3 | 3 | 158 | 15 | 172 | 2.49e-32 | 120 |
| MS.gene23546.t1 | MTR_3g070500 | 47.552 | 143 | 63 | 4 | 8 | 139 | 76 | 217 | 7.86e-32 | 114 |
| MS.gene23546.t1 | MTR_8g442420 | 37.952 | 166 | 95 | 2 | 1 | 158 | 1 | 166 | 1.40e-31 | 119 |
| MS.gene23546.t1 | MTR_3g463900 | 47.794 | 136 | 59 | 4 | 8 | 132 | 46 | 180 | 6.58e-31 | 110 |
| MS.gene23546.t1 | MTR_4g073650 | 44.853 | 136 | 63 | 4 | 8 | 132 | 58 | 192 | 1.72e-27 | 102 |
| MS.gene23546.t1 | MTR_3g070500 | 51.351 | 111 | 42 | 4 | 8 | 107 | 76 | 185 | 1.72e-24 | 94.0 |
| MS.gene23546.t1 | MTR_3g107660 | 39.231 | 130 | 66 | 3 | 15 | 132 | 29 | 157 | 2.03e-21 | 90.1 |
| MS.gene23546.t1 | MTR_1g011980 | 38.462 | 130 | 66 | 4 | 15 | 132 | 26 | 153 | 2.15e-19 | 84.3 |
| MS.gene23546.t1 | MTR_1g094065 | 33.113 | 151 | 93 | 3 | 4 | 153 | 83 | 226 | 1.44e-18 | 79.3 |
| MS.gene23546.t1 | MTR_1g035360 | 33.562 | 146 | 86 | 4 | 4 | 146 | 82 | 219 | 2.70e-18 | 78.6 |
| MS.gene23546.t1 | MTR_3g085705 | 41.237 | 97 | 53 | 2 | 46 | 142 | 33 | 125 | 2.27e-15 | 68.6 |
| MS.gene23546.t1 | MTR_5g039210 | 30.994 | 171 | 95 | 6 | 3 | 158 | 28 | 190 | 1.06e-13 | 65.9 |
| MS.gene23546.t1 | MTR_3g070500 | 47.191 | 89 | 35 | 4 | 8 | 85 | 76 | 163 | 4.91e-13 | 63.5 |
| MS.gene23546.t1 | MTR_5g039210 | 32.192 | 146 | 79 | 5 | 3 | 136 | 28 | 165 | 6.48e-13 | 64.3 |
| MS.gene23546.t1 | MTR_3g070500 | 48.235 | 85 | 32 | 4 | 8 | 81 | 76 | 159 | 7.06e-13 | 63.2 |
| MS.gene23546.t1 | MTR_8g036030 | 100.000 | 27 | 0 | 0 | 134 | 160 | 69 | 95 | 1.72e-12 | 60.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23546.t1 | AT1G01940 | 90.625 | 160 | 15 | 0 | 1 | 160 | 1 | 160 | 1.93e-111 | 313 |
| MS.gene23546.t1 | AT5G67530 | 54.194 | 155 | 71 | 0 | 3 | 157 | 346 | 500 | 8.79e-55 | 182 |
| MS.gene23546.t1 | AT2G36130 | 51.656 | 151 | 73 | 0 | 3 | 153 | 12 | 162 | 8.98e-55 | 170 |
| MS.gene23546.t1 | AT3G44600 | 50.658 | 152 | 75 | 0 | 2 | 153 | 477 | 628 | 2.53e-50 | 170 |
| MS.gene23546.t1 | AT3G63400 | 48.993 | 149 | 62 | 4 | 12 | 148 | 23 | 169 | 3.04e-38 | 134 |
| MS.gene23546.t1 | AT5G13120 | 49.645 | 141 | 66 | 4 | 10 | 146 | 103 | 242 | 7.84e-38 | 130 |
| MS.gene23546.t1 | AT5G13120 | 49.645 | 141 | 66 | 4 | 10 | 146 | 107 | 246 | 8.65e-38 | 130 |
| MS.gene23546.t1 | AT4G33060 | 47.917 | 144 | 71 | 3 | 3 | 143 | 15 | 157 | 8.85e-38 | 135 |
| MS.gene23546.t1 | AT3G63400 | 49.664 | 149 | 61 | 5 | 12 | 148 | 23 | 169 | 5.39e-37 | 133 |
| MS.gene23546.t1 | AT3G63400 | 49.664 | 149 | 61 | 5 | 12 | 148 | 23 | 169 | 5.39e-37 | 133 |
| MS.gene23546.t1 | AT3G63400 | 49.664 | 149 | 61 | 5 | 12 | 148 | 23 | 169 | 5.39e-37 | 133 |
| MS.gene23546.t1 | AT4G34870 | 49.315 | 146 | 58 | 4 | 7 | 139 | 15 | 157 | 1.44e-36 | 124 |
| MS.gene23546.t1 | AT3G56070 | 44.654 | 159 | 75 | 4 | 10 | 157 | 18 | 174 | 3.35e-35 | 121 |
| MS.gene23546.t1 | AT3G56070 | 44.654 | 159 | 75 | 4 | 10 | 157 | 18 | 174 | 3.35e-35 | 121 |
| MS.gene23546.t1 | AT3G62030 | 51.389 | 144 | 63 | 4 | 10 | 148 | 108 | 249 | 3.52e-35 | 123 |
| MS.gene23546.t1 | AT3G62030 | 51.389 | 144 | 63 | 4 | 10 | 148 | 109 | 250 | 3.84e-35 | 123 |
| MS.gene23546.t1 | AT3G62030 | 51.389 | 144 | 63 | 4 | 10 | 148 | 162 | 303 | 1.31e-34 | 123 |
| MS.gene23546.t1 | AT2G21130 | 49.645 | 141 | 59 | 4 | 10 | 139 | 19 | 158 | 2.52e-34 | 119 |
| MS.gene23546.t1 | AT2G15790 | 51.111 | 135 | 53 | 3 | 10 | 132 | 18 | 151 | 8.50e-34 | 122 |
| MS.gene23546.t1 | AT4G38740 | 47.518 | 141 | 62 | 3 | 10 | 139 | 18 | 157 | 9.03e-34 | 117 |
| MS.gene23546.t1 | AT3G55920 | 48.227 | 141 | 61 | 3 | 10 | 139 | 73 | 212 | 4.76e-33 | 117 |
| MS.gene23546.t1 | AT1G53720 | 37.952 | 166 | 95 | 2 | 1 | 158 | 1 | 166 | 1.05e-32 | 119 |
| MS.gene23546.t1 | AT1G53720 | 37.952 | 166 | 95 | 2 | 1 | 158 | 1 | 166 | 5.48e-32 | 119 |
| MS.gene23546.t1 | AT2G16600 | 46.154 | 143 | 61 | 4 | 10 | 139 | 19 | 158 | 3.51e-31 | 110 |
| MS.gene23546.t1 | AT2G29960 | 48.507 | 134 | 57 | 4 | 10 | 132 | 46 | 178 | 4.08e-31 | 111 |
| MS.gene23546.t1 | AT2G29960 | 48.507 | 134 | 57 | 4 | 10 | 132 | 46 | 178 | 4.08e-31 | 111 |
| MS.gene23546.t1 | AT5G58710 | 46.269 | 134 | 60 | 3 | 10 | 132 | 49 | 181 | 9.77e-29 | 105 |
| MS.gene23546.t1 | AT2G38730 | 45.833 | 144 | 65 | 5 | 10 | 142 | 46 | 187 | 2.23e-28 | 104 |
| MS.gene23546.t1 | AT4G34960 | 44.961 | 129 | 59 | 4 | 9 | 126 | 60 | 187 | 1.35e-25 | 97.8 |
| MS.gene23546.t1 | AT2G16600 | 43.182 | 132 | 59 | 3 | 10 | 139 | 19 | 136 | 3.89e-25 | 94.7 |
| MS.gene23546.t1 | AT2G29960 | 44.030 | 134 | 53 | 5 | 10 | 132 | 46 | 168 | 2.38e-24 | 93.6 |
| MS.gene23546.t1 | AT4G34960 | 46.364 | 110 | 47 | 4 | 9 | 107 | 60 | 168 | 4.06e-21 | 84.7 |
| MS.gene23546.t1 | AT4G32420 | 35.135 | 148 | 82 | 5 | 15 | 150 | 26 | 171 | 2.38e-17 | 78.6 |
| MS.gene23546.t1 | AT4G32420 | 35.135 | 148 | 82 | 5 | 15 | 150 | 26 | 171 | 2.38e-17 | 78.6 |
| MS.gene23546.t1 | AT4G32420 | 35.135 | 148 | 82 | 5 | 15 | 150 | 26 | 171 | 2.38e-17 | 78.6 |
| MS.gene23546.t1 | AT4G32420 | 35.135 | 148 | 82 | 5 | 15 | 150 | 26 | 171 | 2.38e-17 | 78.6 |
| MS.gene23546.t1 | AT2G47320 | 32.000 | 150 | 98 | 2 | 4 | 153 | 81 | 226 | 3.58e-17 | 75.9 |
| MS.gene23546.t1 | AT4G32420 | 37.405 | 131 | 69 | 4 | 15 | 133 | 26 | 155 | 1.20e-16 | 76.6 |
| MS.gene23546.t1 | AT1G53780 | 38.202 | 89 | 54 | 1 | 71 | 158 | 4 | 92 | 6.07e-14 | 68.6 |
| MS.gene23546.t1 | AT3G66654 | 27.517 | 149 | 107 | 1 | 5 | 153 | 85 | 232 | 8.89e-12 | 61.2 |
| MS.gene23546.t1 | AT3G66654 | 27.517 | 149 | 107 | 1 | 5 | 153 | 85 | 232 | 8.89e-12 | 61.2 |
| MS.gene23546.t1 | AT3G66654 | 27.517 | 149 | 107 | 1 | 5 | 153 | 85 | 232 | 8.89e-12 | 61.2 |
| MS.gene23546.t1 | AT3G66654 | 27.517 | 149 | 107 | 1 | 5 | 153 | 85 | 232 | 8.89e-12 | 61.2 |
| MS.gene23546.t1 | AT3G66654 | 27.517 | 149 | 107 | 1 | 5 | 153 | 85 | 232 | 8.89e-12 | 61.2 |
| MS.gene23546.t1 | AT1G26940 | 30.986 | 142 | 90 | 4 | 1 | 136 | 1 | 140 | 1.30e-11 | 60.5 |
| MS.gene23546.t1 | AT1G26940 | 31.429 | 140 | 88 | 4 | 3 | 136 | 34 | 171 | 2.12e-11 | 60.1 |
Find 41 sgRNAs with CRISPR-Local
Find 133 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACTGACTTCCATTAGTATT+TGG | 0.166809 | 8.4:+61524580 | None:intergenic |
| ATATTTCACCGCAATATTAA+AGG | 0.210104 | 8.4:-61525187 | MS.gene23546:CDS |
| TTTGTTGTGCAGACTCCAAC+AGG | 0.336703 | 8.4:-61523746 | MS.gene23546:intron |
| AATGGACTATACACCGTGTT+TGG | 0.381087 | 8.4:-61524526 | MS.gene23546:CDS |
| GCAAAACAACCACATTTGAA+TGG | 0.399385 | 8.4:-61524544 | MS.gene23546:CDS |
| ATGCATTCTAATCCACTTGC+TGG | 0.402070 | 8.4:-61523677 | MS.gene23546:CDS |
| GTGTTTGGAAGAGTAATTCA+TGG | 0.424305 | 8.4:-61524511 | MS.gene23546:CDS |
| TGTGCGAGTGGTTACTTTGA+TGG | 0.431366 | 8.4:-61525214 | MS.gene23546:CDS |
| ATTTGAAGTTCTTGATCTCA+TGG | 0.441468 | 8.4:-61524488 | MS.gene23546:CDS |
| GTGCAGACTCCAACAGGGGC+AGG | 0.442734 | 8.4:-61523740 | MS.gene23546:intron |
| CGAGATAAGAGAATCTTTGA+AGG | 0.451647 | 8.4:-61525089 | MS.gene23546:intron |
| GATCCAACTGGTACCGGCAA+AGG | 0.454778 | 8.4:-61525145 | MS.gene23546:CDS |
| ATCCAACTGGTACCGGCAAA+GGG | 0.456047 | 8.4:-61525144 | MS.gene23546:CDS |
| GTGCGAGTGGTTACTTTGAT+GGG | 0.467141 | 8.4:-61525213 | MS.gene23546:CDS |
| AGTTCTTGATCTCATGGAAA+AGG | 0.470855 | 8.4:-61524482 | MS.gene23546:intron |
| AACAGTGGTCCAAATACTAA+TGG | 0.476528 | 8.4:-61524589 | MS.gene23546:CDS |
| GGTCGATCCCCTGCCCCTGT+TGG | 0.500602 | 8.4:+61523731 | None:intergenic |
| ATCGACCACTTGCAGAGATA+AGG | 0.511883 | 8.4:-61523715 | MS.gene23546:CDS |
| GGTGGAGATCCAACTGGTAC+CGG | 0.512107 | 8.4:-61525151 | MS.gene23546:CDS |
| GCAGACTCCAACAGGGGCAG+GGG | 0.516609 | 8.4:-61523738 | MS.gene23546:intron |
| TCATAAAACCTTTAATATTG+CGG | 0.529387 | 8.4:+61525179 | None:intergenic |
| TGCAGACTCCAACAGGGGCA+GGG | 0.545321 | 8.4:-61523739 | MS.gene23546:intron |
| TGCAAGGGGAATATTGTCAA+TGG | 0.546666 | 8.4:-61524614 | MS.gene23546:CDS |
| ATTCAAGGTGGAGATCCAAC+TGG | 0.551039 | 8.4:-61525157 | MS.gene23546:CDS |
| CCAACTGGTACCGGCAAAGG+GGG | 0.557700 | 8.4:-61525142 | MS.gene23546:CDS |
| AGACCAGTCTAGCCAGCAAG+TGG | 0.559274 | 8.4:+61523665 | None:intergenic |
| TCCAACTGGTACCGGCAAAG+GGG | 0.565649 | 8.4:-61525143 | MS.gene23546:CDS |
| TGTTGTGCAGACTCCAACAG+GGG | 0.570681 | 8.4:-61523744 | MS.gene23546:intron |
| TTGTGTTCCAGCATAATGCA+AGG | 0.572411 | 8.4:-61524630 | MS.gene23546:intron |
| TGTGTTCCAGCATAATGCAA+GGG | 0.579477 | 8.4:-61524629 | MS.gene23546:intron |
| GTGTTCCAGCATAATGCAAG+GGG | 0.605603 | 8.4:-61524628 | MS.gene23546:intron |
| TTTCGCATTTGATGTCACCT+AGG | 0.612378 | 8.4:+61525449 | None:intergenic |
| ATGAATTACTCTTCCAAACA+CGG | 0.615509 | 8.4:+61524513 | None:intergenic |
| TTGAGCCTTATCTCTGCAAG+TGG | 0.632274 | 8.4:+61523710 | None:intergenic |
| GTGTATAGTCCATTCAAATG+TGG | 0.635991 | 8.4:+61524535 | None:intergenic |
| TTGTTGTGCAGACTCCAACA+GGG | 0.638282 | 8.4:-61523745 | MS.gene23546:intron |
| GTTACTCTTCACACAAACCT+AGG | 0.645766 | 8.4:-61525466 | MS.gene23546:CDS |
| CAACTGGTACCGGCAAAGGG+GGG | 0.655196 | 8.4:-61525141 | MS.gene23546:CDS |
| ATATTGTCAATGGCTAACAG+TGG | 0.731092 | 8.4:-61524604 | MS.gene23546:CDS |
| GAATGAAGTACGTACCTCGG+AGG | 0.745275 | 8.4:+61525402 | None:intergenic |
| CGAAGTTCCTAAAACCTCCG+AGG | 0.749820 | 8.4:-61525416 | MS.gene23546:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTTGTTTATCAAAAAATTT+GGG | - | chr8.4:61524788-61524807 | MS.gene23546:intron | 10.0% |
| !! | TTATAAAAATATTCATTCAA+TGG | + | chr8.4:61525096-61525115 | None:intergenic | 10.0% |
| !!! | AATTTGTTTATCAAAAAATT+TGG | - | chr8.4:61524787-61524806 | MS.gene23546:intron | 10.0% |
| !! | AAATAGAATGTAATCATTAT+AGG | + | chr8.4:61525270-61525289 | None:intergenic | 15.0% |
| !! | TCATTATAGGATATATTATT+TGG | + | chr8.4:61525257-61525276 | None:intergenic | 15.0% |
| !! | TTTATAAAAACAAACGAAAT+AGG | - | chr8.4:61524259-61524278 | MS.gene23546:intron | 15.0% |
| !!! | TGATTCAATTTTTTAAACAT+TGG | - | chr8.4:61524971-61524990 | MS.gene23546:intron | 15.0% |
| !! | AAACCAAAAATAAACACAAT+AGG | + | chr8.4:61524462-61524481 | None:intergenic | 20.0% |
| !! | TCATAAAACCTTTAATATTG+CGG | + | chr8.4:61524050-61524069 | None:intergenic | 20.0% |
| !! | TTAAAAAATTGAATCAGACA+TGG | + | chr8.4:61524967-61524986 | None:intergenic | 20.0% |
| !!! | ATTAAAGGTTTTATGATTCA+AGG | - | chr8.4:61524054-61524073 | MS.gene23546:intron | 20.0% |
| !!! | TATCCTATTGTGTTTATTTT+TGG | - | chr8.4:61524456-61524475 | MS.gene23546:intron | 20.0% |
| !!! | TTGTGTTTATTTTTGGTTTA+TGG | - | chr8.4:61524463-61524482 | MS.gene23546:intron | 20.0% |
| ! | AAATAACCAAACTGAAGTAA+AGG | + | chr8.4:61524857-61524876 | None:intergenic | 25.0% |
| ! | AATAGTTGAATGTGATCATT+AGG | - | chr8.4:61525050-61525069 | MS.gene23546:intron | 25.0% |
| ! | ATATTTCACCGCAATATTAA+AGG | - | chr8.4:61524039-61524058 | MS.gene23546:intron | 25.0% |
| ! | GGATATATTATTTGGATAGT+TGG | + | chr8.4:61525249-61525268 | None:intergenic | 25.0% |
| !! | CAAGAATTACATGATTTTAC+CGG | + | chr8.4:61523892-61523911 | None:intergenic | 25.0% |
| !! | TCTTTTATTGTTTGTTCAGT+CGG | - | chr8.4:61523738-61523757 | MS.gene23546:intron | 25.0% |
| !!! | CTGTTCAGTTTTTAAAACAT+TGG | + | chr8.4:61524820-61524839 | None:intergenic | 25.0% |
| !!! | TAAACATTGGTTGATGATAA+AGG | - | chr8.4:61524984-61525003 | MS.gene23546:intron | 25.0% |
| !!! | TTTATTTTTGGTTTATGGTG+TGG | - | chr8.4:61524468-61524487 | MS.gene23546:intron | 25.0% |
| !!! | TTTCCACAAAAAAGTATCAA+CGG | - | chr8.4:61525146-61525165 | MS.gene23546:CDS | 25.0% |
| AAGTATCAACGGAGAAAAAT+TGG | - | chr8.4:61525157-61525176 | MS.gene23546:CDS | 30.0% | |
| ACCAATTGAAACAACAGAAT+AGG | + | chr8.4:61523916-61523935 | None:intergenic | 30.0% | |
| AGTATCAACGGAGAAAAATT+GGG | - | chr8.4:61525158-61525177 | MS.gene23546:CDS | 30.0% | |
| ATGAATTACTCTTCCAAACA+CGG | + | chr8.4:61524716-61524735 | None:intergenic | 30.0% | |
| ATGTTTAGTATTTGAGTGCA+TGG | + | chr8.4:61525431-61525450 | None:intergenic | 30.0% | |
| CCAATTGAAACAACAGAATA+GGG | + | chr8.4:61523915-61523934 | None:intergenic | 30.0% | |
| GTATAACAGATTGCCTATAT+TGG | + | chr8.4:61524355-61524374 | None:intergenic | 30.0% | |
| TAGAATTGACAAAGTATACG+CGG | - | chr8.4:61524285-61524304 | MS.gene23546:intron | 30.0% | |
| TAGTTACATCACTCCAATAT+AGG | - | chr8.4:61524339-61524358 | MS.gene23546:intron | 30.0% | |
| TGGGTTAAAGGTCAATATTA+CGG | - | chr8.4:61524395-61524414 | MS.gene23546:intron | 30.0% | |
| TTGAATGTGATCATTAGGAA+TGG | - | chr8.4:61525055-61525074 | MS.gene23546:intron | 30.0% | |
| ! | AACTGACTTCCATTAGTATT+TGG | + | chr8.4:61524649-61524668 | None:intergenic | 30.0% |
| ! | ATTTGAAGTTCTTGATCTCA+TGG | - | chr8.4:61524738-61524757 | MS.gene23546:intron | 30.0% |
| ! | GTTTGATCACAATTTTGTCT+CGG | - | chr8.4:61524882-61524901 | MS.gene23546:intron | 30.0% |
| ! | TTGATTTGACAACACAATGA+AGG | - | chr8.4:61525320-61525339 | MS.gene23546:intron | 30.0% |
| !! | AAAGGTTTTATGATTCAAGG+TGG | - | chr8.4:61524057-61524076 | MS.gene23546:intron | 30.0% |
| AAACATAGCCTACAATTCTG+AGG | + | chr8.4:61525014-61525033 | None:intergenic | 35.0% | |
| AACAGTGGTCCAAATACTAA+TGG | - | chr8.4:61524637-61524656 | MS.gene23546:intron | 35.0% | |
| AATTGAATCAGACATGGAAC+AGG | + | chr8.4:61524961-61524980 | None:intergenic | 35.0% | |
| AGTTCTTGATCTCATGGAAA+AGG | - | chr8.4:61524744-61524763 | MS.gene23546:intron | 35.0% | |
| ATATTGTCAATGGCTAACAG+TGG | - | chr8.4:61524622-61524641 | MS.gene23546:CDS | 35.0% | |
| ATGTCCCAGTATGCTTAAAA+AGG | + | chr8.4:61524502-61524521 | None:intergenic | 35.0% | |
| ATGTGATCATTAGGAATGGA+AGG | - | chr8.4:61525059-61525078 | MS.gene23546:intron | 35.0% | |
| CGAAAACTAATGAATCGTGA+TGG | + | chr8.4:61525218-61525237 | None:intergenic | 35.0% | |
| CGAGATAAGAGAATCTTTGA+AGG | - | chr8.4:61524137-61524156 | MS.gene23546:intron | 35.0% | |
| GCAAAACAACCACATTTGAA+TGG | - | chr8.4:61524682-61524701 | MS.gene23546:intron | 35.0% | |
| GTGTATAGTCCATTCAAATG+TGG | + | chr8.4:61524694-61524713 | None:intergenic | 35.0% | |
| GTGTTTGGAAGAGTAATTCA+TGG | - | chr8.4:61524715-61524734 | MS.gene23546:intron | 35.0% | |
| GTTAAGCATATGTTAGAGCA+GGG | - | chr8.4:61524200-61524219 | MS.gene23546:intron | 35.0% | |
| TCAGAATTGTAGGCTATGTT+TGG | - | chr8.4:61525013-61525032 | MS.gene23546:intron | 35.0% | |
| TGTCTCCTCAGTTATCTTAA+CGG | - | chr8.4:61523857-61523876 | MS.gene23546:intron | 35.0% | |
| TGTGATCATTAGGAATGGAA+GGG | - | chr8.4:61525060-61525079 | MS.gene23546:intron | 35.0% | |
| TGTTAAGCATATGTTAGAGC+AGG | - | chr8.4:61524199-61524218 | MS.gene23546:intron | 35.0% | |
| TTAAAGCAGAAACCTTGAAC+TGG | - | chr8.4:61524915-61524934 | MS.gene23546:intron | 35.0% | |
| TTAAGCATATGTTAGAGCAG+GGG | - | chr8.4:61524201-61524220 | MS.gene23546:intron | 35.0% | |
| ! | CTTTAACCCAATAAAAGCGT+CGG | + | chr8.4:61524385-61524404 | None:intergenic | 35.0% |
| ! | GTTTCTTCATCAGATTTTCG+TGG | - | chr8.4:61524552-61524571 | MS.gene23546:CDS | 35.0% |
| ! | TAAGTTCCGACGCTTTTATT+GGG | - | chr8.4:61524376-61524395 | MS.gene23546:intron | 35.0% |
| ! | TTCACTTGTTCAGTATTGGA+TGG | - | chr8.4:61525184-61525203 | MS.gene23546:CDS | 35.0% |
| !! | CCCTATTCTGTTGTTTCAAT+TGG | - | chr8.4:61523912-61523931 | MS.gene23546:intron | 35.0% |
| !! | CTTCAGTTTGGTTATTTTCG+TGG | - | chr8.4:61524860-61524879 | MS.gene23546:intron | 35.0% |
| !!! | TCTCCGTTGATACTTTTTTG+TGG | + | chr8.4:61525152-61525171 | None:intergenic | 35.0% |
| AAGGTGAACCTCAGAATTGT+AGG | - | chr8.4:61525003-61525022 | MS.gene23546:intron | 40.0% | |
| ACTGAACAGAAACCTTGAAC+TGG | - | chr8.4:61524831-61524850 | MS.gene23546:intron | 40.0% | |
| AGTGAATGAAGTACGTACCT+CGG | + | chr8.4:61523830-61523849 | None:intergenic | 40.0% | |
| ATATTCCCCTTGCATTATGC+TGG | + | chr8.4:61524606-61524625 | None:intergenic | 40.0% | |
| ATGCATTCTAATCCACTTGC+TGG | - | chr8.4:61525549-61525568 | MS.gene23546:intron | 40.0% | |
| CATCAATCCAAACATAGCCT+GGG | - | chr8.4:61525290-61525309 | MS.gene23546:intron | 40.0% | |
| GCTGTTCACTTGTTCAGTAT+TGG | - | chr8.4:61525180-61525199 | MS.gene23546:CDS | 40.0% | |
| GGGACATTTCTTGTGCTTTA+TGG | - | chr8.4:61524515-61524534 | MS.gene23546:CDS | 40.0% | |
| GTCTCCTCAGTTATCTTAAC+GGG | - | chr8.4:61523858-61523877 | MS.gene23546:intron | 40.0% | |
| GTTACTCTTCACACAAACCT+AGG | - | chr8.4:61523760-61523779 | MS.gene23546:intron | 40.0% | |
| TCATCAATCCAAACATAGCC+TGG | - | chr8.4:61525289-61525308 | MS.gene23546:intron | 40.0% | |
| TGCAAGGGGAATATTGTCAA+TGG | - | chr8.4:61524612-61524631 | MS.gene23546:CDS | 40.0% | |
| TGTGTTCCAGCATAATGCAA+GGG | - | chr8.4:61524597-61524616 | MS.gene23546:CDS | 40.0% | |
| TTGTGTTCCAGCATAATGCA+AGG | - | chr8.4:61524596-61524615 | MS.gene23546:CDS | 40.0% | |
| TTTCGCATTTGATGTCACCT+AGG | + | chr8.4:61523780-61523799 | None:intergenic | 40.0% | |
| TTTGAGTAAAGGCCAGTTCA+AGG | + | chr8.4:61524930-61524949 | None:intergenic | 40.0% | |
| ! | AGATTTTCGTGGTCGCTAAT+TGG | - | chr8.4:61524563-61524582 | MS.gene23546:CDS | 40.0% |
| ! | CGACGCTTTTATTGGGTTAA+AGG | - | chr8.4:61524383-61524402 | MS.gene23546:intron | 40.0% |
| ! | CTAAGTTCCGACGCTTTTAT+TGG | - | chr8.4:61524375-61524394 | MS.gene23546:intron | 40.0% |
| ! | TTTGGTTTATGGTGTGGACT+GGG | - | chr8.4:61524474-61524493 | MS.gene23546:intron | 40.0% |
| !! | AACTGGCCTTTACTTCAGTT+TGG | - | chr8.4:61524848-61524867 | MS.gene23546:intron | 40.0% |
| !! | AATGGACTATACACCGTGTT+TGG | - | chr8.4:61524700-61524719 | MS.gene23546:intron | 40.0% |
| !! | AGGCAAGACCTTTTGAGTAA+AGG | + | chr8.4:61524941-61524960 | None:intergenic | 40.0% |
| !! | CAATAAAAGCGTCGGAACTT+AGG | + | chr8.4:61524377-61524396 | None:intergenic | 40.0% |
| !! | CTCAGTTATCTTAACGGGTT+CGG | - | chr8.4:61523863-61523882 | MS.gene23546:intron | 40.0% |
| !! | GGGACCTTTTTAAGCATACT+GGG | - | chr8.4:61524495-61524514 | MS.gene23546:CDS | 40.0% |
| !! | TCAGTTATCTTAACGGGTTC+GGG | - | chr8.4:61523864-61523883 | MS.gene23546:intron | 40.0% |
| !! | TGAACTGGCCTTTACTCAAA+AGG | - | chr8.4:61524930-61524949 | MS.gene23546:intron | 40.0% |
| !!! | TTTTGGTTTATGGTGTGGAC+TGG | - | chr8.4:61524473-61524492 | MS.gene23546:intron | 40.0% |
| ATCGACCACTTGCAGAGATA+AGG | - | chr8.4:61525511-61525530 | MS.gene23546:intron | 45.0% | |
| ATTCAAGGTGGAGATCCAAC+TGG | - | chr8.4:61524069-61524088 | MS.gene23546:intron | 45.0% | |
| CGAACCCGTTAAGATAACTG+AGG | + | chr8.4:61523865-61523884 | None:intergenic | 45.0% | |
| CTGAAGTAAAGGCCAGTTCA+AGG | + | chr8.4:61524846-61524865 | None:intergenic | 45.0% | |
| GTCAAATCAAGTCAAGTCCC+AGG | + | chr8.4:61525310-61525329 | None:intergenic | 45.0% | |
| GTGTGTGTGAAATCTAGACG+AGG | + | chr8.4:61525409-61525428 | None:intergenic | 45.0% | |
| GTGTTCCAGCATAATGCAAG+GGG | - | chr8.4:61524598-61524617 | MS.gene23546:CDS | 45.0% | |
| TTGTTGTGCAGACTCCAACA+GGG | - | chr8.4:61525481-61525500 | MS.gene23546:intron | 45.0% | |
| ! | AAAGGGGGGACTAGTATATG+GGG | - | chr8.4:61524099-61524118 | MS.gene23546:intron | 45.0% |
| ! | CAAAGGGGGGACTAGTATAT+GGG | - | chr8.4:61524098-61524117 | MS.gene23546:intron | 45.0% |
| ! | GTGCGAGTGGTTACTTTGAT+GGG | - | chr8.4:61524013-61524032 | MS.gene23546:intron | 45.0% |
| ! | TGTGCGAGTGGTTACTTTGA+TGG | - | chr8.4:61524012-61524031 | MS.gene23546:intron | 45.0% |
| ! | TTGAGCCTTATCTCTGCAAG+TGG | + | chr8.4:61525519-61525538 | None:intergenic | 45.0% |
| ! | TTGGTTTATGGTGTGGACTG+GGG | - | chr8.4:61524475-61524494 | MS.gene23546:intron | 45.0% |
| ! | TTTGTTGTGCAGACTCCAAC+AGG | - | chr8.4:61525480-61525499 | MS.gene23546:intron | 45.0% |
| !! | GGGGACCTTTTTAAGCATAC+TGG | - | chr8.4:61524494-61524513 | MS.gene23546:CDS | 45.0% |
| !! | TTTTTAGCGCTTTGTGCGAG+TGG | - | chr8.4:61524000-61524019 | MS.gene23546:intron | 45.0% |
| CGAAGTTCCTAAAACCTCCG+AGG | - | chr8.4:61523810-61523829 | MS.gene23546:intron | 50.0% | |
| CGCGGTTTGACTGAAATCGA+TGG | - | chr8.4:61524303-61524322 | MS.gene23546:intron | 50.0% | |
| CTACACACACAAGCATAGCG+CGG | + | chr8.4:61524175-61524194 | None:intergenic | 50.0% | |
| GAATGAAGTACGTACCTCGG+AGG | + | chr8.4:61523827-61523846 | None:intergenic | 50.0% | |
| GTCAAGTCCCAGGCTATGTT+TGG | + | chr8.4:61525300-61525319 | None:intergenic | 50.0% | |
| TATACTAGTCCCCCCTTTGC+CGG | + | chr8.4:61524097-61524116 | None:intergenic | 50.0% | |
| TGTTGTGCAGACTCCAACAG+GGG | - | chr8.4:61525482-61525501 | MS.gene23546:intron | 50.0% | |
| ! | GCAAAGGGGGGACTAGTATA+TGG | - | chr8.4:61524097-61524116 | MS.gene23546:intron | 50.0% |
| ! | GTACGTACCTCGGAGGTTTT+AGG | + | chr8.4:61523820-61523839 | None:intergenic | 50.0% |
| !! | ATCCAACTGGTACCGGCAAA+GGG | - | chr8.4:61524082-61524101 | MS.gene23546:intron | 50.0% |
| !! | ATCTTAACGGGTTCGGGTTC+CGG | - | chr8.4:61523870-61523889 | MS.gene23546:intron | 50.0% |
| GGTGGAGATCCAACTGGTAC+CGG | - | chr8.4:61524075-61524094 | MS.gene23546:intron | 55.0% | |
| !! | GATCCAACTGGTACCGGCAA+AGG | - | chr8.4:61524081-61524100 | MS.gene23546:intron | 55.0% |
| !! | TCCAACTGGTACCGGCAAAG+GGG | - | chr8.4:61524083-61524102 | MS.gene23546:intron | 55.0% |
| CACACACAAGCATAGCGCGG+CGG | + | chr8.4:61524172-61524191 | None:intergenic | 60.0% | |
| TGCAGACTCCAACAGGGGCA+GGG | - | chr8.4:61525487-61525506 | MS.gene23546:intron | 60.0% | |
| !! | CAACTGGTACCGGCAAAGGG+GGG | - | chr8.4:61524085-61524104 | MS.gene23546:intron | 60.0% |
| !! | CCAACTGGTACCGGCAAAGG+GGG | - | chr8.4:61524084-61524103 | MS.gene23546:intron | 60.0% |
| CCCCCTTTGCCGGTACCAGT+TGG | + | chr8.4:61524087-61524106 | None:intergenic | 65.0% | |
| GCAGACTCCAACAGGGGCAG+GGG | - | chr8.4:61525488-61525507 | MS.gene23546:intron | 65.0% | |
| GTGCAGACTCCAACAGGGGC+AGG | - | chr8.4:61525486-61525505 | MS.gene23546:intron | 65.0% | |
| GGTCGATCCCCTGCCCCTGT+TGG | + | chr8.4:61525498-61525517 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 61523673 | 61525575 | 61523673 | ID=MS.gene23546 |
| chr8.4 | mRNA | 61523673 | 61525575 | 61523673 | ID=MS.gene23546.t1;Parent=MS.gene23546 |
| chr8.4 | exon | 61525573 | 61525575 | 61525573 | ID=MS.gene23546.t1.exon1;Parent=MS.gene23546.t1 |
| chr8.4 | CDS | 61525573 | 61525575 | 61525573 | ID=cds.MS.gene23546.t1;Parent=MS.gene23546.t1 |
| chr8.4 | exon | 61525417 | 61525491 | 61525417 | ID=MS.gene23546.t1.exon2;Parent=MS.gene23546.t1 |
| chr8.4 | CDS | 61525417 | 61525491 | 61525417 | ID=cds.MS.gene23546.t1;Parent=MS.gene23546.t1 |
| chr8.4 | exon | 61525090 | 61525251 | 61525090 | ID=MS.gene23546.t1.exon3;Parent=MS.gene23546.t1 |
| chr8.4 | CDS | 61525090 | 61525251 | 61525090 | ID=cds.MS.gene23546.t1;Parent=MS.gene23546.t1 |
| chr8.4 | exon | 61524483 | 61524641 | 61524483 | ID=MS.gene23546.t1.exon4;Parent=MS.gene23546.t1 |
| chr8.4 | CDS | 61524483 | 61524641 | 61524483 | ID=cds.MS.gene23546.t1;Parent=MS.gene23546.t1 |
| chr8.4 | exon | 61523673 | 61523756 | 61523673 | ID=MS.gene23546.t1.exon5;Parent=MS.gene23546.t1 |
| chr8.4 | CDS | 61523673 | 61523756 | 61523673 | ID=cds.MS.gene23546.t1;Parent=MS.gene23546.t1 |
| Gene Sequence |
| Protein sequence |
