AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene038261

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene038261.t1 MTR_5g090660 83.951 324 20 2 1 292 1 324 0.0 545
MS.gene038261.t1 MTR_5g090660 98.630 73 1 0 293 365 403 475 2.66e-40 150
MS.gene038261.t1 MTR_5g090600 61.269 457 86 7 1 366 1 457 0.0 514
MS.gene038261.t1 MTR_5g090670 70.427 328 61 4 1 292 1 328 4.56e-156 447
MS.gene038261.t1 MTR_5g090670 86.486 74 10 0 293 366 407 480 1.24e-34 133
MS.gene038261.t1 MTR_5g090630 70.062 324 58 3 1 292 1 317 6.82e-154 441
MS.gene038261.t1 MTR_5g090630 86.486 74 10 0 293 366 396 469 1.66e-35 135
MS.gene038261.t1 MTR_5g090610 68.098 326 80 3 1 302 1 326 2.18e-151 435
MS.gene038261.t1 MTR_5g090610 82.432 74 13 0 293 366 391 464 1.52e-32 127
MS.gene038261.t1 MTR_5g090700 68.293 328 68 4 1 292 1 328 3.67e-151 435
MS.gene038261.t1 MTR_5g090700 82.432 74 13 0 293 366 407 480 5.97e-33 128
MS.gene038261.t1 MTR_5g090580 69.301 329 67 5 1 295 1 329 3.06e-149 430
MS.gene038261.t1 MTR_5g090580 83.784 74 12 0 293 366 405 478 6.92e-34 131
MS.gene038261.t1 MTR_5g090620 65.443 327 78 5 1 292 1 327 2.38e-140 407
MS.gene038261.t1 MTR_5g090620 82.432 74 13 0 293 366 406 479 3.38e-35 134
MS.gene038261.t1 MTR_5g090650 59.327 327 70 5 1 295 1 296 1.41e-124 362
MS.gene038261.t1 MTR_5g098930 59.091 330 92 5 1 295 1 322 1.84e-124 367
MS.gene038261.t1 MTR_5g098930 68.919 74 23 0 293 366 398 471 2.31e-26 109
MS.gene038261.t1 MTR_5g090690 48.320 387 75 7 65 356 18 374 1.51e-89 274
MS.gene038261.t1 MTR_5g070040 38.978 313 150 10 2 276 8 317 2.08e-60 202
MS.gene038261.t1 MTR_2g046750 38.080 323 160 10 1 287 1 319 2.71e-56 191
MS.gene038261.t1 MTR_2g040530 36.474 329 168 10 1 293 1 324 3.94e-53 182
MS.gene038261.t1 MTR_5g070090 37.117 326 151 13 2 281 8 325 6.26e-53 182
MS.gene038261.t1 MTR_2g046820 35.780 327 167 10 1 291 1 320 2.25e-50 175
MS.gene038261.t1 MTR_3g009510 31.361 338 186 11 1 295 1 335 4.97e-40 147
MS.gene038261.t1 MTR_6g488280 29.114 316 180 9 3 282 4 311 5.68e-33 128
MS.gene038261.t1 MTR_0428s0010 29.114 316 180 9 3 282 4 311 5.68e-33 128
MS.gene038261.t1 MTR_6g023665 28.616 318 181 10 2 282 10 318 1.35e-32 127
MS.gene038261.t1 MTR_8g465670 29.677 310 173 10 2 275 4 304 1.53e-32 127
MS.gene038261.t1 MTR_8g006220 29.677 310 173 10 2 275 3 303 3.25e-32 126
MS.gene038261.t1 MTR_7g117170 30.968 310 163 11 6 275 16 314 1.27e-29 119
MS.gene038261.t1 MTR_8g006730 27.760 317 173 10 2 275 3 306 1.94e-29 118
MS.gene038261.t1 MTR_8g006270 28.896 308 177 9 2 275 3 302 4.49e-29 117
MS.gene038261.t1 MTR_8g027870 31.023 303 166 9 6 275 19 311 8.47e-29 117
MS.gene038261.t1 MTR_8g027885 30.363 303 168 9 6 275 19 311 1.27e-28 116
MS.gene038261.t1 MTR_8g006710 26.752 314 179 11 2 275 3 305 2.63e-28 115
MS.gene038261.t1 MTR_0184s0040 26.129 310 187 7 2 275 18 321 2.04e-27 113
MS.gene038261.t1 MTR_0078s0190 26.129 310 187 7 2 275 18 321 2.04e-27 113
MS.gene038261.t1 MTR_0078s0060 26.537 309 186 8 2 275 3 305 1.68e-25 107
MS.gene038261.t1 MTR_6g033675 23.579 475 232 16 3 360 2 462 1.68e-25 107
MS.gene038261.t1 MTR_8g009050 28.981 314 172 12 2 275 3 305 2.27e-25 107
MS.gene038261.t1 MTR_8g009063 28.846 312 171 11 2 273 10 310 6.58e-25 105
MS.gene038261.t1 MTR_8g009073 27.900 319 169 11 2 275 3 305 7.18e-25 105
MS.gene038261.t1 MTR_8g077590 28.438 320 162 14 4 275 8 308 1.08e-24 105
MS.gene038261.t1 MTR_0078s0200 26.603 312 184 9 2 275 3 307 1.45e-24 102
MS.gene038261.t1 MTR_8g009055 28.205 312 177 11 2 275 3 305 3.62e-24 103
MS.gene038261.t1 MTR_0184s0030 26.129 310 188 8 2 275 3 307 6.61e-24 102
MS.gene038261.t1 MTR_8g006260 27.103 321 187 10 3 283 4 317 8.04e-24 102
MS.gene038261.t1 MTR_1g019510 25.153 326 205 8 2 294 3 322 1.14e-23 102
MS.gene038261.t1 MTR_2g098430 28.440 327 167 16 2 275 3 315 1.40e-23 102
MS.gene038261.t1 MTR_0078s0010 25.478 314 184 10 2 275 3 306 2.01e-23 101
MS.gene038261.t1 MTR_8g008970 27.044 318 187 9 2 282 3 312 2.74e-23 102
MS.gene038261.t1 MTR_8g009067 27.244 312 180 10 2 275 3 305 5.01e-23 100
MS.gene038261.t1 MTR_8g008990 28.939 311 176 10 2 275 3 305 6.38e-23 100
MS.gene038261.t1 MTR_7g047030 24.920 313 187 12 2 275 3 306 3.70e-22 97.8
MS.gene038261.t1 MTR_7g047270 24.920 313 187 12 2 275 3 306 3.70e-22 97.8
MS.gene038261.t1 MTR_8g074550 25.402 311 186 10 2 273 5 308 6.80e-22 97.4
MS.gene038261.t1 MTR_8g012635 26.730 318 177 11 2 275 3 308 2.25e-21 95.5
MS.gene038261.t1 MTR_7g046490 28.527 319 164 12 2 275 3 302 3.01e-21 95.1
MS.gene038261.t1 MTR_6g005600 24.679 312 198 7 3 281 4 311 3.10e-21 95.1
MS.gene038261.t1 MTR_8g006230 27.468 233 134 6 75 275 18 247 4.38e-21 92.4
MS.gene038261.t1 MTR_5g019580 25.538 325 190 11 2 282 4 320 4.47e-21 94.7
MS.gene038261.t1 MTR_6g033615 26.403 303 184 9 3 273 2 297 7.15e-21 94.0
MS.gene038261.t1 MTR_6g033625 26.814 317 179 13 3 273 2 311 8.45e-21 93.6
MS.gene038261.t1 MTR_6g038240 26.129 310 178 9 6 274 12 311 2.40e-20 92.4
MS.gene038261.t1 MTR_8g012745 26.048 334 186 11 2 287 3 323 5.76e-20 91.3
MS.gene038261.t1 MTR_6g033725 25.080 311 189 10 3 273 2 308 2.31e-19 89.7
MS.gene038261.t1 MTR_4g485590 28.065 310 187 12 4 287 8 307 2.39e-19 89.0
MS.gene038261.t1 MTR_5g019580 26.974 304 184 12 2 282 56 344 1.03e-18 87.8
MS.gene038261.t1 MTR_7g080900 23.913 322 175 9 2 273 9 310 9.90e-18 84.7
MS.gene038261.t1 MTR_6g033660 21.598 463 249 15 6 360 5 461 1.26e-17 84.3
MS.gene038261.t1 MTR_8g012650 26.733 202 110 5 109 275 110 308 4.32e-17 82.8
MS.gene038261.t1 MTR_5g035580 24.194 310 182 8 5 274 13 309 4.46e-17 82.8
MS.gene038261.t1 MTR_8g466240 25.305 328 188 13 3 287 6 319 6.31e-17 82.0
MS.gene038261.t1 MTR_6g038300 24.522 314 178 10 5 273 11 310 9.97e-17 81.6
MS.gene038261.t1 MTR_5g072810 24.510 306 186 8 4 273 11 307 1.06e-16 81.6
MS.gene038261.t1 MTR_7g080935 22.581 310 191 9 3 273 9 308 1.42e-16 81.3
MS.gene038261.t1 MTR_1g107360 21.767 464 252 16 5 365 9 464 1.98e-16 80.9
MS.gene038261.t1 MTR_3g009500 26.642 274 155 11 1 233 1 269 2.83e-16 78.6
MS.gene038261.t1 MTR_0536s0020 25.658 304 181 11 2 270 15 308 3.93e-16 80.1
MS.gene038261.t1 MTR_5g464760 25.228 329 184 9 3 273 10 334 4.37e-16 79.7
MS.gene038261.t1 MTR_5g090680 80.952 42 8 0 1 42 1 42 4.91e-16 73.9
MS.gene038261.t1 MTR_5g035560 24.204 314 177 10 5 274 11 307 9.11e-16 79.0
MS.gene038261.t1 MTR_5g029800 26.537 309 172 12 5 270 11 307 1.25e-15 78.6
MS.gene038261.t1 MTR_8g066610 24.633 341 185 13 6 287 10 337 1.68e-15 78.2
MS.gene038261.t1 MTR_8g009067 28.421 190 98 6 120 275 2 187 2.61e-15 76.6
MS.gene038261.t1 MTR_5g072840 25.083 303 185 9 4 273 9 302 2.63e-15 77.4
MS.gene038261.t1 MTR_4g095008 23.636 330 191 9 6 289 12 326 3.83e-15 77.0
MS.gene038261.t1 MTR_7g080950 25.078 319 181 11 3 278 4 307 7.57e-15 75.9
MS.gene038261.t1 MTR_3g069690 22.884 319 177 9 2 273 5 301 1.50e-14 75.1
MS.gene038261.t1 MTR_6g038200 22.293 314 189 8 4 274 10 311 1.63e-14 74.7
MS.gene038261.t1 MTR_3g009520 26.667 255 141 10 2 214 3 253 2.34e-14 72.8
MS.gene038261.t1 MTR_4g082810 37.000 100 58 2 179 273 212 311 2.41e-14 74.3
MS.gene038261.t1 MTR_7g080940 21.290 310 195 8 3 273 9 308 2.65e-14 74.3
MS.gene038261.t1 MTR_4g082820 23.899 318 180 12 5 273 4 308 6.29e-14 73.2
MS.gene038261.t1 MTR_6g078320 22.188 320 195 11 4 273 10 325 6.47e-14 73.2
MS.gene038261.t1 MTR_8g088560 24.194 310 179 10 5 270 10 307 7.52e-14 72.8
MS.gene038261.t1 MTR_5g072850 25.362 276 170 10 5 273 10 256 1.05e-13 72.0
MS.gene038261.t1 MTR_8g009073 35.922 103 58 4 178 275 77 176 1.15e-13 71.6
MS.gene038261.t1 MTR_5g090590 65.306 49 17 0 296 344 6 54 1.20e-13 65.5
MS.gene038261.t1 MTR_8g064870 23.197 319 197 9 5 280 11 324 1.31e-13 72.4
MS.gene038261.t1 MTR_8g012730 32.432 111 56 1 165 275 54 145 1.56e-13 70.9
MS.gene038261.t1 MTR_6g038230 22.293 314 189 8 4 274 10 311 1.62e-13 72.0
MS.gene038261.t1 MTR_7g070660 24.611 321 177 16 5 274 11 317 2.31e-13 71.6
MS.gene038261.t1 MTR_3g069710 23.701 308 187 9 2 273 9 304 2.35e-13 71.2
MS.gene038261.t1 MTR_7g070870 27.811 169 97 5 120 279 172 324 3.73e-13 70.9
MS.gene038261.t1 MTR_6g038220 23.585 318 175 11 6 274 12 310 4.21e-13 70.5
MS.gene038261.t1 MTR_4g117960 22.654 309 180 15 8 270 14 309 4.86e-13 70.5
MS.gene038261.t1 MTR_1g029540 23.183 289 190 9 10 273 19 300 5.06e-13 70.5
MS.gene038261.t1 MTR_5g087620 23.353 334 189 13 2 275 7 333 5.48e-13 70.5
MS.gene038261.t1 MTR_4g117890 25.000 308 178 13 2 270 10 303 6.65e-13 70.1
MS.gene038261.t1 MTR_4g117980 23.779 307 181 10 8 270 14 311 1.03e-12 69.3
MS.gene038261.t1 MTR_4g094920 21.450 331 199 8 5 289 26 341 1.52e-12 68.9
MS.gene038261.t1 MTR_6g035185 24.839 310 174 16 8 270 14 311 1.52e-12 68.9
MS.gene038261.t1 MTR_1g107285 23.988 321 185 10 5 275 8 319 1.80e-12 68.6
MS.gene038261.t1 MTR_2g035020 23.323 313 181 13 5 270 11 311 3.61e-12 67.8
MS.gene038261.t1 MTR_6g035265 22.591 301 183 11 8 270 14 302 4.15e-12 67.8
MS.gene038261.t1 MTR_7g071040 22.699 326 187 12 3 274 15 329 7.11e-12 67.0
MS.gene038261.t1 MTR_4g117950 25.890 309 173 13 2 270 3 295 8.68e-12 66.6
MS.gene038261.t1 MTR_4g118000 23.649 296 182 8 8 270 15 299 1.20e-11 66.2
MS.gene038261.t1 MTR_4g031800 24.762 315 178 12 2 270 6 307 1.23e-11 66.2
MS.gene038261.t1 MTR_7g102520 22.257 319 189 10 7 273 14 325 1.25e-11 66.2
MS.gene038261.t1 MTR_7g070610 27.219 169 96 4 120 274 169 324 1.31e-11 66.2
MS.gene038261.t1 MTR_8g083290 24.771 327 169 11 6 273 5 313 1.57e-11 65.9
MS.gene038261.t1 MTR_4g008630 24.917 301 181 11 6 273 15 303 3.17e-11 64.7
MS.gene038261.t1 MTR_1g032490 22.500 320 195 11 5 278 10 322 4.25e-11 64.3
MS.gene038261.t1 MTR_8g083280 24.773 331 176 13 5 278 7 321 4.69e-11 64.3
MS.gene038261.t1 MTR_1g062380 21.429 322 191 11 6 274 17 329 7.02e-11 63.9
MS.gene038261.t1 MTR_1g107350 22.601 323 197 10 5 281 8 323 8.97e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene038261.t1 AT3G21750 36.446 332 162 8 3 295 2 323 4.83e-63 209
MS.gene038261.t1 AT3G21790 32.927 328 174 5 3 287 2 326 6.57e-56 191
MS.gene038261.t1 AT3G21760 37.273 330 160 9 3 287 2 329 7.89e-56 190
MS.gene038261.t1 AT3G21760 43.243 74 42 0 293 366 411 484 2.51e-13 71.2
MS.gene038261.t1 AT4G15280 34.862 327 165 9 3 287 2 322 4.57e-55 188
MS.gene038261.t1 AT4G15280 34.862 327 165 9 3 287 34 354 6.42e-55 188
MS.gene038261.t1 AT1G07240 30.472 466 207 14 1 351 1 464 1.96e-54 186
MS.gene038261.t1 AT3G21780 34.259 324 166 8 3 287 2 317 7.22e-53 182
MS.gene038261.t1 AT3G21800 33.939 330 168 7 1 287 1 323 7.60e-53 182
MS.gene038261.t1 AT1G07250 34.375 320 161 10 1 273 2 319 1.89e-48 170
MS.gene038261.t1 AT2G29730 34.286 315 164 9 1 274 1 313 2.58e-46 164
MS.gene038261.t1 AT2G29740 34.743 331 167 14 2 286 5 332 1.37e-44 160
MS.gene038261.t1 AT1G07260 31.515 330 176 11 3 286 4 329 2.40e-43 157
MS.gene038261.t1 AT2G29750 32.880 368 196 15 2 322 5 368 1.27e-42 155
MS.gene038261.t1 AT4G15260 38.278 209 85 6 116 287 1 202 3.56e-40 145
MS.gene038261.t1 AT2G29710 34.030 335 158 13 1 281 1 326 3.63e-39 145
MS.gene038261.t1 AT3G16520 29.430 316 177 8 1 276 1 310 2.41e-36 137
MS.gene038261.t1 AT3G16520 29.430 316 177 8 1 276 1 310 2.74e-36 137
MS.gene038261.t1 AT3G16520 30.063 316 175 9 1 276 1 310 3.16e-36 137
MS.gene038261.t1 AT4G01070 28.664 307 182 7 2 275 5 307 2.49e-31 122
MS.gene038261.t1 AT4G01070 28.664 307 182 7 2 275 5 307 1.52e-30 122
MS.gene038261.t1 AT4G36770 29.221 308 171 10 8 278 7 304 5.82e-25 105
MS.gene038261.t1 AT4G15270 37.013 154 88 4 3 149 2 153 6.25e-25 101
MS.gene038261.t1 AT5G26310 29.206 315 167 14 12 287 14 311 6.53e-25 105
MS.gene038261.t1 AT4G15270 37.013 154 88 4 3 149 2 153 9.31e-25 101
MS.gene038261.t1 AT4G15270 36.306 157 91 4 3 152 2 156 2.91e-24 101
MS.gene038261.t1 AT1G01390 27.213 305 183 9 5 275 25 324 5.72e-24 103
MS.gene038261.t1 AT1G01390 26.557 305 185 8 5 275 8 307 6.22e-24 103
MS.gene038261.t1 AT5G66690 25.641 312 176 11 12 283 14 309 1.49e-23 102
MS.gene038261.t1 AT5G12890 28.086 324 175 15 2 275 7 322 2.34e-23 101
MS.gene038261.t1 AT1G01420 25.902 305 187 7 5 275 8 307 8.51e-23 99.8
MS.gene038261.t1 AT1G01420 25.902 305 187 7 5 275 11 310 8.98e-23 99.8
MS.gene038261.t1 AT2G18570 24.038 312 194 8 1 275 1 306 1.24e-22 99.4
MS.gene038261.t1 AT3G50740 24.432 352 202 13 1 310 3 332 1.31e-22 99.4
MS.gene038261.t1 AT2G15480 25.532 329 190 9 2 279 7 331 7.97e-18 85.1
MS.gene038261.t1 AT2G15480 25.532 329 190 9 2 279 7 331 1.02e-17 84.7
MS.gene038261.t1 AT4G34131 26.667 330 175 12 2 274 7 326 1.73e-17 84.0
MS.gene038261.t1 AT2G30150 24.918 305 183 7 5 273 13 307 5.68e-16 79.3
MS.gene038261.t1 AT4G34135 25.231 325 186 11 2 274 8 327 1.27e-15 77.4
MS.gene038261.t1 AT1G24100 25.369 339 188 12 3 290 9 333 1.41e-15 78.2
MS.gene038261.t1 AT2G18560 34.951 103 64 1 177 276 115 217 2.07e-15 77.4
MS.gene038261.t1 AT4G34135 25.231 325 186 11 2 274 8 327 2.74e-15 77.4
MS.gene038261.t1 AT2G30150 24.585 301 181 7 9 273 1 291 3.00e-15 77.0
MS.gene038261.t1 AT2G36750 24.214 318 181 11 7 273 12 320 5.38e-15 76.6
MS.gene038261.t1 AT4G15480 25.225 222 121 5 95 276 111 327 5.62e-15 76.6
MS.gene038261.t1 AT2G31790 22.812 320 186 11 2 274 5 310 5.78e-15 76.3
MS.gene038261.t1 AT2G15490 24.085 328 196 10 2 279 4 328 9.86e-15 75.9
MS.gene038261.t1 AT3G46650 24.161 298 193 12 3 280 8 292 2.51e-14 74.3
MS.gene038261.t1 AT2G15490 24.149 323 192 10 2 274 4 323 2.67e-14 74.3
MS.gene038261.t1 AT4G15490 24.926 337 193 13 2 288 5 331 2.98e-14 74.3
MS.gene038261.t1 AT4G15500 24.681 235 135 6 94 289 97 328 3.70e-14 73.9
MS.gene038261.t1 AT3G02100 22.086 326 184 11 1 270 9 320 4.17e-14 73.6
MS.gene038261.t1 AT4G15550 23.453 307 183 9 7 270 15 312 1.54e-13 72.0
MS.gene038261.t1 AT3G55710 23.810 336 173 11 2 280 5 314 3.10e-13 71.2
MS.gene038261.t1 AT2G23250 37.815 119 59 3 165 273 175 288 4.14e-13 70.5
MS.gene038261.t1 AT3G55700 35.398 113 65 3 172 280 202 310 1.08e-12 69.3
MS.gene038261.t1 AT4G34138 25.664 339 187 14 3 287 9 336 1.70e-12 68.9
MS.gene038261.t1 AT2G31750 23.757 362 201 14 3 315 6 341 1.96e-12 68.6
MS.gene038261.t1 AT3G46670 23.642 313 187 12 6 280 10 308 2.05e-12 68.6
MS.gene038261.t1 AT2G36800 22.188 320 187 10 7 273 14 324 2.10e-12 68.6
MS.gene038261.t1 AT2G31750 23.757 362 201 14 3 315 6 341 2.28e-12 68.6
MS.gene038261.t1 AT2G43820 24.841 314 186 11 2 278 4 304 2.51e-12 68.2
MS.gene038261.t1 AT2G36780 24.138 319 181 14 7 273 16 325 3.07e-12 68.2
MS.gene038261.t1 AT2G43840 24.351 308 183 9 3 273 5 299 3.26e-12 67.8
MS.gene038261.t1 AT1G73880 23.620 326 178 12 3 275 12 319 3.62e-12 67.8
MS.gene038261.t1 AT2G43840 24.351 308 183 9 3 273 5 299 3.77e-12 67.8
MS.gene038261.t1 AT2G23260 24.922 321 171 10 2 273 7 306 7.59e-12 66.6
MS.gene038261.t1 AT3G46660 24.351 308 185 13 3 273 12 308 1.33e-11 66.2

Find 107 sgRNAs with CRISPR-Local

Find 160 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCGAAATTGCGTTGGATTAT+AGG 0.154357 5.4:+71555515 MS.gene038261:CDS
TTTCCTCTGCTTCGGGAGTA+TGG 0.212424 5.4:+71555120 MS.gene038261:CDS
GTCATCGGTGCCCTTGGTTT+TGG 0.226170 5.4:-71555056 None:intergenic
GGGTCTTCTTACGCTTATTT+AGG 0.232646 5.4:+71555682 MS.gene038261:CDS
CGAAATTGCGTTGGATTATA+GGG 0.280259 5.4:+71555516 MS.gene038261:CDS
TTTGGGCTGCAGGTTCAGTA+TGG 0.283478 5.4:-71555038 None:intergenic
AGGAACCTGGATCGGTGTTT+GGG 0.292144 5.4:-71554535 None:intergenic
GATTATAGGGCGGAGTTTAA+TGG 0.293163 5.4:+71555529 MS.gene038261:CDS
GGTTGATTATATTATGAATC+AGG 0.301103 5.4:+71555711 MS.gene038261:CDS
TATTCCTCTCTCAATCTTAT+CGG 0.302069 5.4:-71555575 None:intergenic
GAGGAACCTGGATCGGTGTT+TGG 0.330268 5.4:-71554536 None:intergenic
ACACTTGTCTTCTTCACTTC+CGG 0.334459 5.4:+71554710 MS.gene038261:CDS
CTCACTACCAGATCAGAAAA+TGG 0.344871 5.4:+71554614 MS.gene038261:CDS
ATAAGATTGAGAGAGGAATA+AGG 0.350503 5.4:+71555578 MS.gene038261:CDS
AGTCGTTTATGATCGGCTAT+TGG 0.353780 5.4:+71554894 MS.gene038261:CDS
TCATCTGATCCTGGTTTAGC+TGG 0.360401 5.4:+71554992 MS.gene038261:CDS
GATACGGCCTGTTTGATGTT+TGG 0.373158 5.4:-71554587 None:intergenic
ACTTCCGGTGTTGCGTTCCT+TGG 0.377556 5.4:+71554725 MS.gene038261:CDS
TTACGCTTATTTAGGTCGTT+TGG 0.383098 5.4:+71555690 MS.gene038261:CDS
ATGTGGAGTGAATCGGAGAT+AGG 0.394209 5.4:-71554485 None:intergenic
GGGTTCTTTCAATGAGGATC+AGG 0.411413 5.4:+71555141 MS.gene038261:CDS
TCGGCTGTTACAAGATAGTT+AGG 0.415501 5.4:-71555556 None:intergenic
AATAAGGAGTGTGTTGGATA+AGG 0.419886 5.4:+71555594 MS.gene038261:CDS
ATGGGTCCTACCGGATATAT+AGG 0.432997 5.4:-71555019 None:intergenic
TGTAGGCACGGAGAACTCTT+TGG 0.437195 5.4:-71554690 None:intergenic
AGCTGGTTTACCTATATATC+CGG 0.438856 5.4:+71555009 MS.gene038261:CDS
TTCTTTACTTAGCACAGCAC+TGG 0.441792 5.4:-71554867 None:intergenic
AGTAAGAAAACATTGTTAGA+AGG 0.447750 5.4:+71555658 MS.gene038261:CDS
TTGGGCTGCAGGTTCAGTAT+GGG 0.448617 5.4:-71555037 None:intergenic
AGATGACCTGCAGCCGGAAA+AGG 0.451791 5.4:-71554344 None:intergenic
TTCCTCTGCTTCGGGAGTAT+GGG 0.453583 5.4:+71555121 MS.gene038261:CDS
GCATGCGCTATTGAGAATAG+TGG 0.454195 5.4:+71555175 MS.gene038261:CDS
GGCGCGGCCATGGTGCCCTC+TGG 0.455189 5.4:-71555232 None:intergenic
AGGAGTGTGTTGGATAAGGA+TGG 0.460546 5.4:+71555598 MS.gene038261:CDS
TGCTGTGCTAAGTAAAGAAT+GGG 0.468763 5.4:+71554871 MS.gene038261:CDS
GCGGCCATGGTGCCCTCTGG+TGG 0.475512 5.4:-71555229 None:intergenic
ATAGTGGGGCCATGTTCATA+TGG 0.477659 5.4:+71555191 MS.gene038261:CDS
TTAGAAGCTTCGCGAACTCA+AGG 0.484420 5.4:-71554376 None:intergenic
GCCATGGTGCCCTCTGGTGG+AGG 0.487632 5.4:-71555226 None:intergenic
CATCGGTTTCTGTGGTGTGC+GGG 0.494308 5.4:-71554445 None:intergenic
GAGAGGAATAAGGAGTGTGT+TGG 0.494443 5.4:+71555588 MS.gene038261:CDS
GATGACCTGCAGCCGGAAAA+GGG 0.500182 5.4:-71554343 None:intergenic
GTGCTGTGCTAAGTAAAGAA+TGG 0.501914 5.4:+71554870 MS.gene038261:CDS
TCAGAAACCAAACATCAAAC+AGG 0.507875 5.4:+71554580 MS.gene038261:CDS
GTAATCAGAGGGCGCGGCCA+TGG 0.518576 5.4:-71555242 None:intergenic
GATGATGTCATCGGTGCCCT+TGG 0.518830 5.4:-71555062 None:intergenic
TGGATAAGGATGGAGATGTA+AGG 0.521344 5.4:+71555608 MS.gene038261:CDS
CAAACCAAGGAACGCAACAC+CGG 0.528558 5.4:-71554729 None:intergenic
AAGAAAACATTGTTAGAAGG+TGG 0.530165 5.4:+71555661 MS.gene038261:CDS
AATGGGAGTCGAAATTGCGT+TGG 0.531788 5.4:+71555507 MS.gene038261:intron
CATGCGCTATTGAGAATAGT+GGG 0.532838 5.4:+71555176 MS.gene038261:CDS
TGTTTGGTTTCTGAGCTTCG+AGG 0.540247 5.4:-71554571 None:intergenic
AAAGATGAAAACTCAAACCA+AGG 0.543276 5.4:-71554742 None:intergenic
GTTGATGATGTGGAGTGAAT+CGG 0.547542 5.4:-71554492 None:intergenic
GGAGTGAATCGGAGATAGGA+AGG 0.557005 5.4:-71554481 None:intergenic
TTCGAGGAGCGCAGTCATAG+AGG 0.560869 5.4:-71554555 None:intergenic
TAAGATGAAGAAAGCAGAAG+TGG 0.563991 5.4:+71554313 None:intergenic
ACATCGGTTTCTGTGGTGTG+CGG 0.568243 5.4:-71554446 None:intergenic
TGATGATCAACCTCCTTCCT+CGG 0.570079 5.4:+71555093 MS.gene038261:CDS
TAGAAGCTTCGCGAACTCAA+GGG 0.570470 5.4:-71554375 None:intergenic
TTACTCGGAGACGGTTATCA+CGG 0.575806 5.4:-71554403 None:intergenic
TTTATGATCGGCTATTGGAA+AGG 0.576864 5.4:+71554899 MS.gene038261:CDS
AGAAAACATTGTTAGAAGGT+GGG 0.577699 5.4:+71555662 MS.gene038261:CDS
AGGTTCAGTATGGGTCCTAC+CGG 0.580031 5.4:-71555028 None:intergenic
GAATGGGAGTCGTTTATGAT+CGG 0.585661 5.4:+71554887 MS.gene038261:CDS
GAAGAAGACAAGTGTAGGCA+CGG 0.585980 5.4:-71554702 None:intergenic
CAGAGGAAAACTACCGAGGA+AGG 0.586007 5.4:-71555106 None:intergenic
AGTGTAGACATCGGTTTCTG+TGG 0.587465 5.4:-71554453 None:intergenic
AACCCATACTCCCGAAGCAG+AGG 0.589515 5.4:-71555123 None:intergenic
TCTTTACTTAGCACAGCACT+GGG 0.589923 5.4:-71554866 None:intergenic
GGAACCTGGATCGGTGTTTG+GGG 0.593092 5.4:-71554534 None:intergenic
TCTGGTAGTGAGGTTAGATA+CGG 0.594098 5.4:-71554603 None:intergenic
TGAAAACGAGTGAACTGCAT+GGG 0.595427 5.4:-71554972 None:intergenic
AAAGGCCTCAAGAATGCTGA+TGG 0.596518 5.4:+71554917 MS.gene038261:CDS
GAAACTAGATGACCTGCAGC+CGG 0.597871 5.4:-71554350 None:intergenic
AAGCCACTTGATGATGTCAT+CGG 0.604822 5.4:-71555071 None:intergenic
GAACATGTCAACAACGAAAG+CGG 0.605630 5.4:-71554645 None:intergenic
AGCGCAGTCATAGAGGAACC+TGG 0.606391 5.4:-71554548 None:intergenic
TAAAGCCATCAGCATTCTTG+AGG 0.611334 5.4:-71554922 None:intergenic
AAGAGGGTAATCAGAGGGCG+CGG 0.615621 5.4:-71555248 None:intergenic
GGGGAAGAGAACATTCTGGA+AGG 0.616011 5.4:-71554514 None:intergenic
AGTCATAGAGGAACCTGGAT+CGG 0.617905 5.4:-71554543 None:intergenic
GGTTTACCTATATATCCGGT+AGG 0.620912 5.4:+71555013 MS.gene038261:CDS
GAGTATGGGTTCTTTCAATG+AGG 0.621177 5.4:+71555135 MS.gene038261:CDS
TGATAACTAAAACAGTTACT+CGG 0.623393 5.4:-71554418 None:intergenic
ACCTCCACCAGAGGGCACCA+TGG 0.624215 5.4:+71555225 MS.gene038261:CDS
GAACCTGCAGCCCAAAACCA+AGG 0.624605 5.4:+71555045 MS.gene038261:CDS
ATTCTGGAAGGTTGATGATG+TGG 0.626981 5.4:-71554502 None:intergenic
ATAGGTAAACCAGCTAAACC+AGG 0.627999 5.4:-71555001 None:intergenic
AACCTGCAGCCCAAAACCAA+GGG 0.629871 5.4:+71555046 MS.gene038261:CDS
GAAGTGAAGAAGACAAGTGT+AGG 0.632023 5.4:-71554707 None:intergenic
CTAAAACAGTTACTCGGAGA+CGG 0.634129 5.4:-71554412 None:intergenic
TCTTCGTAAACCTCCACCAG+AGG 0.637133 5.4:+71555216 MS.gene038261:CDS
AAGCTTCGCGAACTCAAGGG+CGG 0.641179 5.4:-71554372 None:intergenic
ATGAAAACGAGTGAACTGCA+TGG 0.644548 5.4:-71554973 None:intergenic
GAGTGAATCGGAGATAGGAA+GGG 0.654524 5.4:-71554480 None:intergenic
ACAGCCGATAAGATTGAGAG+AGG 0.655724 5.4:+71555571 MS.gene038261:CDS
TGTCAACAACGAAAGCGGCA+AGG 0.657453 5.4:-71554640 None:intergenic
AGGAAAACTACCGAGGAAGG+AGG 0.665308 5.4:-71555103 None:intergenic
CTTCGTAAACCTCCACCAGA+GGG 0.665923 5.4:+71555217 MS.gene038261:CDS
GCACCGATGACATCATCAAG+TGG 0.677327 5.4:+71555068 MS.gene038261:CDS
AAGGGACTTAGTGTAGACAT+CGG 0.680660 5.4:-71554462 None:intergenic
ACGAAGAGACCATATGAACA+TGG 0.683117 5.4:-71555200 None:intergenic
GAACCTGGATCGGTGTTTGG+GGG 0.690042 5.4:-71554533 None:intergenic
AATTGCGTTGGATTATAGGG+CGG 0.699009 5.4:+71555519 MS.gene038261:CDS
GAAGCAGAGGAAAACTACCG+AGG 0.711128 5.4:-71555110 None:intergenic
ATGCGCTATTGAGAATAGTG+GGG 0.737124 5.4:+71555177 MS.gene038261:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AGTAAGAAAACATTGTTAGA+AGG + chr5.4:71555658-71555677 MS.gene038261:CDS 25.0%
! GGTTGATTATATTATGAATC+AGG + chr5.4:71555711-71555730 MS.gene038261:CDS 25.0%
! TGATAACTAAAACAGTTACT+CGG - chr5.4:71554421-71554440 None:intergenic 25.0%
AAAGATGAAAACTCAAACCA+AGG - chr5.4:71554745-71554764 None:intergenic 30.0%
AAGAAAACATTGTTAGAAGG+TGG + chr5.4:71555661-71555680 MS.gene038261:CDS 30.0%
AGAAAACATTGTTAGAAGGT+GGG + chr5.4:71555662-71555681 MS.gene038261:CDS 30.0%
ATAAGATTGAGAGAGGAATA+AGG + chr5.4:71555578-71555597 MS.gene038261:CDS 30.0%
TATTCCTCTCTCAATCTTAT+CGG - chr5.4:71555578-71555597 None:intergenic 30.0%
TTGAACCCAAATCAAAAAGA+GGG - chr5.4:71555267-71555286 None:intergenic 30.0%
! ACAGACTAATGCTTTTGAAT+TGG + chr5.4:71555471-71555490 MS.gene038261:intron 30.0%
!! TGATTACCCTCTTTTTGATT+TGG + chr5.4:71555258-71555277 MS.gene038261:CDS 30.0%
!!! GATTACCCTCTTTTTGATTT+GGG + chr5.4:71555259-71555278 MS.gene038261:CDS 30.0%
!!! GTACTTGAGAGCATTTATTT+TGG + chr5.4:71555412-71555431 MS.gene038261:intron 30.0%
ATCAAAAAGAGGGTAATCAG+AGG - chr5.4:71555257-71555276 None:intergenic 35.0%
CGAAATTGCGTTGGATTATA+GGG + chr5.4:71555516-71555535 MS.gene038261:CDS 35.0%
CTTGAACCCAAATCAAAAAG+AGG - chr5.4:71555268-71555287 None:intergenic 35.0%
GAACCAAGTTAGTGAAACTT+GGG - chr5.4:71554832-71554851 None:intergenic 35.0%
TCAAAAAGAGGGTAATCAGA+GGG - chr5.4:71555256-71555275 None:intergenic 35.0%
TCAGAAACCAAACATCAAAC+AGG + chr5.4:71554580-71554599 MS.gene038261:CDS 35.0%
TCGAAATTGCGTTGGATTAT+AGG + chr5.4:71555515-71555534 MS.gene038261:CDS 35.0%
TGCTGTGCTAAGTAAAGAAT+GGG + chr5.4:71554871-71554890 MS.gene038261:CDS 35.0%
TTACGCTTATTTAGGTCGTT+TGG + chr5.4:71555690-71555709 MS.gene038261:CDS 35.0%
TTTATGATCGGCTATTGGAA+AGG + chr5.4:71554899-71554918 MS.gene038261:CDS 35.0%
! AATAAGGAGTGTGTTGGATA+AGG + chr5.4:71555594-71555613 MS.gene038261:CDS 35.0%
! AGCTGGTTTACCTATATATC+CGG + chr5.4:71555009-71555028 MS.gene038261:CDS 35.0%
! ATTGGTGTGTGAGTTGAAAA+TGG + chr5.4:71555489-71555508 MS.gene038261:intron 35.0%
! TTGGTGTGTGAGTTGAAAAT+GGG + chr5.4:71555490-71555509 MS.gene038261:intron 35.0%
AAGGGACTTAGTGTAGACAT+CGG - chr5.4:71554465-71554484 None:intergenic 40.0%
AATTGCGTTGGATTATAGGG+CGG + chr5.4:71555519-71555538 MS.gene038261:CDS 40.0%
ACACTTGTCTTCTTCACTTC+CGG + chr5.4:71554710-71554729 MS.gene038261:CDS 40.0%
ACGAAGAGACCATATGAACA+TGG - chr5.4:71555203-71555222 None:intergenic 40.0%
AGTCGTTTATGATCGGCTAT+TGG + chr5.4:71554894-71554913 MS.gene038261:CDS 40.0%
ATAGGTAAACCAGCTAAACC+AGG - chr5.4:71555004-71555023 None:intergenic 40.0%
ATGAAAACGAGTGAACTGCA+TGG - chr5.4:71554976-71554995 None:intergenic 40.0%
ATGCGCTATTGAGAATAGTG+GGG + chr5.4:71555177-71555196 MS.gene038261:CDS 40.0%
CAAGTTAGTGAAACTTGGGA+CGG - chr5.4:71554828-71554847 None:intergenic 40.0%
CATGCGCTATTGAGAATAGT+GGG + chr5.4:71555176-71555195 MS.gene038261:CDS 40.0%
CTAAAACAGTTACTCGGAGA+CGG - chr5.4:71554415-71554434 None:intergenic 40.0%
CTCACTACCAGATCAGAAAA+TGG + chr5.4:71554614-71554633 MS.gene038261:CDS 40.0%
GAACATGTCAACAACGAAAG+CGG - chr5.4:71554648-71554667 None:intergenic 40.0%
GAAGTGAAGAAGACAAGTGT+AGG - chr5.4:71554710-71554729 None:intergenic 40.0%
GAATGGGAGTCGTTTATGAT+CGG + chr5.4:71554887-71554906 MS.gene038261:CDS 40.0%
GAGTATGGGTTCTTTCAATG+AGG + chr5.4:71555135-71555154 MS.gene038261:CDS 40.0%
GATTATAGGGCGGAGTTTAA+TGG + chr5.4:71555529-71555548 MS.gene038261:CDS 40.0%
GGAACCAAGTTAGTGAAACT+TGG - chr5.4:71554833-71554852 None:intergenic 40.0%
GGGTCTTCTTACGCTTATTT+AGG + chr5.4:71555682-71555701 MS.gene038261:CDS 40.0%
GGTTTACCTATATATCCGGT+AGG + chr5.4:71555013-71555032 MS.gene038261:CDS 40.0%
GTCTGTTGTTCTGCATAAAG+CGG - chr5.4:71555457-71555476 None:intergenic 40.0%
GTGCTGTGCTAAGTAAAGAA+TGG + chr5.4:71554870-71554889 MS.gene038261:CDS 40.0%
TAAAGCCATCAGCATTCTTG+AGG - chr5.4:71554925-71554944 None:intergenic 40.0%
TCGGCTGTTACAAGATAGTT+AGG - chr5.4:71555559-71555578 None:intergenic 40.0%
TCTTTACTTAGCACAGCACT+GGG - chr5.4:71554869-71554888 None:intergenic 40.0%
TGAAAACGAGTGAACTGCAT+GGG - chr5.4:71554975-71554994 None:intergenic 40.0%
TGGATAAGGATGGAGATGTA+AGG + chr5.4:71555608-71555627 MS.gene038261:CDS 40.0%
TTCTTTACTTAGCACAGCAC+TGG - chr5.4:71554870-71554889 None:intergenic 40.0%
! AAGGACTCCATTTTCTGATC+TGG - chr5.4:71554624-71554643 None:intergenic 40.0%
! GTTGTTACAACTCACTGACT+TGG + chr5.4:71554802-71554821 MS.gene038261:intron 40.0%
! TCTGGTAGTGAGGTTAGATA+CGG - chr5.4:71554606-71554625 None:intergenic 40.0%
!! AAGCCACTTGATGATGTCAT+CGG - chr5.4:71555074-71555093 None:intergenic 40.0%
!! ATTCTGGAAGGTTGATGATG+TGG - chr5.4:71554505-71554524 None:intergenic 40.0%
!! CATTTTCTGATCTGGTAGTG+AGG - chr5.4:71554616-71554635 None:intergenic 40.0%
!! GTTGATGATGTGGAGTGAAT+CGG - chr5.4:71554495-71554514 None:intergenic 40.0%
!! TACCCGAAGGATTTTTAGAG+CGG + chr5.4:71555290-71555309 MS.gene038261:intron 40.0%
!! TGTCCGCTCTAAAAATCCTT+CGG - chr5.4:71555296-71555315 None:intergenic 40.0%
AAAGGCCTCAAGAATGCTGA+TGG + chr5.4:71554917-71554936 MS.gene038261:CDS 45.0%
AAGCAAATCCTCCAATGGCT+GGG - chr5.4:71555372-71555391 None:intergenic 45.0%
AATGGGAGTCGAAATTGCGT+TGG + chr5.4:71555507-71555526 MS.gene038261:intron 45.0%
ACAGCCGATAAGATTGAGAG+AGG + chr5.4:71555571-71555590 MS.gene038261:CDS 45.0%
AGTCATAGAGGAACCTGGAT+CGG - chr5.4:71554546-71554565 None:intergenic 45.0%
ATGCGAAGCAAATCCTCCAA+TGG - chr5.4:71555377-71555396 None:intergenic 45.0%
ATGGGTCCTACCGGATATAT+AGG - chr5.4:71555022-71555041 None:intergenic 45.0%
ATGTGGAGTGAATCGGAGAT+AGG - chr5.4:71554488-71554507 None:intergenic 45.0%
CGTCCCAAGTTTCACTAACT+TGG + chr5.4:71554826-71554845 MS.gene038261:intron 45.0%
GAAGAAGACAAGTGTAGGCA+CGG - chr5.4:71554705-71554724 None:intergenic 45.0%
GAGTGAATCGGAGATAGGAA+GGG - chr5.4:71554483-71554502 None:intergenic 45.0%
GATACGGCCTGTTTGATGTT+TGG - chr5.4:71554590-71554609 None:intergenic 45.0%
GCATGCGCTATTGAGAATAG+TGG + chr5.4:71555175-71555194 MS.gene038261:CDS 45.0%
GGCTAGTATTTGGACTTGTG+GGG - chr5.4:71555347-71555366 None:intergenic 45.0%
GGGTTCTTTCAATGAGGATC+AGG + chr5.4:71555141-71555160 MS.gene038261:CDS 45.0%
TAGAAGCTTCGCGAACTCAA+GGG - chr5.4:71554378-71554397 None:intergenic 45.0%
TCATCTGATCCTGGTTTAGC+TGG + chr5.4:71554992-71555011 MS.gene038261:CDS 45.0%
TGATGATCAACCTCCTTCCT+CGG + chr5.4:71555093-71555112 MS.gene038261:CDS 45.0%
TTACTCGGAGACGGTTATCA+CGG - chr5.4:71554406-71554425 None:intergenic 45.0%
TTAGAAGCTTCGCGAACTCA+AGG - chr5.4:71554379-71554398 None:intergenic 45.0%
TTTGGGGGAAGAGAACATTC+TGG - chr5.4:71554521-71554540 None:intergenic 45.0%
! AGGAGTGTGTTGGATAAGGA+TGG + chr5.4:71555598-71555617 MS.gene038261:CDS 45.0%
! AGTGGTGTTTGTCCCTTTTC+CGG + chr5.4:71554331-71554350 MS.gene038261:CDS 45.0%
! ATAGTGGGGCCATGTTCATA+TGG + chr5.4:71555191-71555210 MS.gene038261:CDS 45.0%
! CACTCGTTTTCATCTGATCC+TGG + chr5.4:71554983-71555002 MS.gene038261:CDS 45.0%
! GAGAGGAATAAGGAGTGTGT+TGG + chr5.4:71555588-71555607 MS.gene038261:CDS 45.0%
! GATTTGCTTCGCATTGTGGT+TGG + chr5.4:71555383-71555402 MS.gene038261:intron 45.0%
! GGGCTAGTATTTGGACTTGT+GGG - chr5.4:71555348-71555367 None:intergenic 45.0%
! TCGGTAGTTTTCCTCTGCTT+CGG + chr5.4:71555112-71555131 MS.gene038261:CDS 45.0%
! TGTTTGGTTTCTGAGCTTCG+AGG - chr5.4:71554574-71554593 None:intergenic 45.0%
! TTGGGTTCAAGTTTACCCGA+AGG + chr5.4:71555277-71555296 MS.gene038261:intron 45.0%
!! AGTGTAGACATCGGTTTCTG+TGG - chr5.4:71554456-71554475 None:intergenic 45.0%
!! GTCCGCTCTAAAAATCCTTC+GGG - chr5.4:71555295-71555314 None:intergenic 45.0%
!! TGGGCTAGTATTTGGACTTG+TGG - chr5.4:71555349-71555368 None:intergenic 45.0%
AAATCCTCCAATGGCTGGGT+GGG - chr5.4:71555368-71555387 None:intergenic 50.0%
AACCTGCAGCCCAAAACCAA+GGG + chr5.4:71555046-71555065 MS.gene038261:CDS 50.0%
AGATTGGAAGGGTCGTTGGA+TGG + chr5.4:71555320-71555339 MS.gene038261:intron 50.0%
AGGAAAACTACCGAGGAAGG+AGG - chr5.4:71555106-71555125 None:intergenic 50.0%
AGGTTCAGTATGGGTCCTAC+CGG - chr5.4:71555031-71555050 None:intergenic 50.0%
CAAACCAAGGAACGCAACAC+CGG - chr5.4:71554732-71554751 None:intergenic 50.0%
CAGAGGAAAACTACCGAGGA+AGG - chr5.4:71555109-71555128 None:intergenic 50.0%
CTTCGTAAACCTCCACCAGA+GGG + chr5.4:71555217-71555236 MS.gene038261:CDS 50.0%
GAAACTAGATGACCTGCAGC+CGG - chr5.4:71554353-71554372 None:intergenic 50.0%
GAAGCAAATCCTCCAATGGC+TGG - chr5.4:71555373-71555392 None:intergenic 50.0%
GAAGCAGAGGAAAACTACCG+AGG - chr5.4:71555113-71555132 None:intergenic 50.0%
GATTGGAAGGGTCGTTGGAT+GGG + chr5.4:71555321-71555340 MS.gene038261:intron 50.0%
GGAGTGAATCGGAGATAGGA+AGG - chr5.4:71554484-71554503 None:intergenic 50.0%
GGGGAAGAGAACATTCTGGA+AGG - chr5.4:71554517-71554536 None:intergenic 50.0%
TAAAGCGGCCATGTGGCTAT+AGG - chr5.4:71555442-71555461 None:intergenic 50.0%
TCTTCGTAAACCTCCACCAG+AGG + chr5.4:71555216-71555235 MS.gene038261:CDS 50.0%
TGTAGGCACGGAGAACTCTT+TGG - chr5.4:71554693-71554712 None:intergenic 50.0%
TGTCAACAACGAAAGCGGCA+AGG - chr5.4:71554643-71554662 None:intergenic 50.0%
TTAGAGCGGACAGCAGAGAT+TGG + chr5.4:71555304-71555323 MS.gene038261:intron 50.0%
TTCTGCATAAAGCGGCCATG+TGG - chr5.4:71555449-71555468 None:intergenic 50.0%
TTGGGCTGCAGGTTCAGTAT+GGG - chr5.4:71555040-71555059 None:intergenic 50.0%
TTGGTGTGCCTATAGCCACA+TGG + chr5.4:71555431-71555450 MS.gene038261:intron 50.0%
TTTCCTCTGCTTCGGGAGTA+TGG + chr5.4:71555120-71555139 MS.gene038261:CDS 50.0%
TTTGGGCTGCAGGTTCAGTA+TGG - chr5.4:71555041-71555060 None:intergenic 50.0%
! AGGAACCTGGATCGGTGTTT+GGG - chr5.4:71554538-71554557 None:intergenic 50.0%
! CGGTAGTTTTCCTCTGCTTC+GGG + chr5.4:71555113-71555132 MS.gene038261:CDS 50.0%
! GCACCGATGACATCATCAAG+TGG + chr5.4:71555068-71555087 MS.gene038261:CDS 50.0%
! GGAGGATTTGCTTCGCATTG+TGG + chr5.4:71555379-71555398 MS.gene038261:intron 50.0%
! TTCCTCTGCTTCGGGAGTAT+GGG + chr5.4:71555121-71555140 MS.gene038261:CDS 50.0%
!! ACATCGGTTTCTGTGGTGTG+CGG - chr5.4:71554449-71554468 None:intergenic 50.0%
!! TCATCGGTGCCCTTGGTTTT+GGG - chr5.4:71555058-71555077 None:intergenic 50.0%
AACCCATACTCCCGAAGCAG+AGG - chr5.4:71555126-71555145 None:intergenic 55.0%
AAGAGGGTAATCAGAGGGCG+CGG - chr5.4:71555251-71555270 None:intergenic 55.0%
AAGCTTCGCGAACTCAAGGG+CGG - chr5.4:71554375-71554394 None:intergenic 55.0%
AGATGACCTGCAGCCGGAAA+AGG - chr5.4:71554347-71554366 None:intergenic 55.0%
AGCGCAGTCATAGAGGAACC+TGG - chr5.4:71554551-71554570 None:intergenic 55.0%
AGCGGACAGCAGAGATTGGA+AGG + chr5.4:71555308-71555327 MS.gene038261:intron 55.0%
ATACTAGCCCACCCAGCCAT+TGG + chr5.4:71555358-71555377 MS.gene038261:intron 55.0%
CAAATCCTCCAATGGCTGGG+TGG - chr5.4:71555369-71555388 None:intergenic 55.0%
GAACCTGCAGCCCAAAACCA+AGG + chr5.4:71555045-71555064 MS.gene038261:CDS 55.0%
GATGACCTGCAGCCGGAAAA+GGG - chr5.4:71554346-71554365 None:intergenic 55.0%
GCAGAGATTGGAAGGGTCGT+TGG + chr5.4:71555316-71555335 MS.gene038261:intron 55.0%
GCGGACAGCAGAGATTGGAA+GGG + chr5.4:71555309-71555328 MS.gene038261:intron 55.0%
! GAACCTGGATCGGTGTTTGG+GGG - chr5.4:71554536-71554555 None:intergenic 55.0%
! GAGGAACCTGGATCGGTGTT+TGG - chr5.4:71554539-71554558 None:intergenic 55.0%
! GATGATGTCATCGGTGCCCT+TGG - chr5.4:71555065-71555084 None:intergenic 55.0%
! GCACTGGGCAACGCTTTTGA+TGG - chr5.4:71554854-71554873 None:intergenic 55.0%
! GGAACCTGGATCGGTGTTTG+GGG - chr5.4:71554537-71554556 None:intergenic 55.0%
! TTCGAGGAGCGCAGTCATAG+AGG - chr5.4:71554558-71554577 None:intergenic 55.0%
! TTTGTCCCTTTTCCGGCTGC+AGG + chr5.4:71554338-71554357 MS.gene038261:CDS 55.0%
!! ACTTCCGGTGTTGCGTTCCT+TGG + chr5.4:71554725-71554744 MS.gene038261:CDS 55.0%
!! CATCGGTTTCTGTGGTGTGC+GGG - chr5.4:71554448-71554467 None:intergenic 55.0%
!! GTCATCGGTGCCCTTGGTTT+TGG - chr5.4:71555059-71555078 None:intergenic 55.0%
!! TGGCTGGGTGGGCTAGTATT+TGG - chr5.4:71555357-71555376 None:intergenic 55.0%
CTTCCCCCAAACACCGATCC+AGG + chr5.4:71554530-71554549 MS.gene038261:CDS 60.0%
!!! TGCCCTTGGTTTTGGGCTGC+AGG - chr5.4:71555051-71555070 None:intergenic 60.0%
CTAGCCCACCCAGCCATTGG+AGG + chr5.4:71555361-71555380 MS.gene038261:intron 65.0%
! GTAATCAGAGGGCGCGGCCA+TGG - chr5.4:71555245-71555264 None:intergenic 65.0%
!! ACCTCCACCAGAGGGCACCA+TGG + chr5.4:71555225-71555244 MS.gene038261:CDS 65.0%
GCCATGGTGCCCTCTGGTGG+AGG - chr5.4:71555229-71555248 None:intergenic 70.0%
GCGGCCATGGTGCCCTCTGG+TGG - chr5.4:71555232-71555251 None:intergenic 75.0%
! GGCGCGGCCATGGTGCCCTC+TGG - chr5.4:71555235-71555254 None:intergenic 80.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 71554317 71555738 71554317 ID=MS.gene038261
chr5.4 mRNA 71554317 71555738 71554317 ID=MS.gene038261.t1;Parent=MS.gene038261
chr5.4 exon 71554317 71554773 71554317 ID=MS.gene038261.t1.exon1;Parent=MS.gene038261.t1
chr5.4 CDS 71554317 71554773 71554317 ID=cds.MS.gene038261.t1;Parent=MS.gene038261.t1
chr5.4 exon 71554861 71555280 71554861 ID=MS.gene038261.t1.exon2;Parent=MS.gene038261.t1
chr5.4 CDS 71554861 71555280 71554861 ID=cds.MS.gene038261.t1;Parent=MS.gene038261.t1
chr5.4 exon 71555515 71555738 71555515 ID=MS.gene038261.t1.exon3;Parent=MS.gene038261.t1
chr5.4 CDS 71555515 71555738 71555515 ID=cds.MS.gene038261.t1;Parent=MS.gene038261.t1
Gene Sequence

>MS.gene038261

ATGAAGAAAGCAGAAGTGGTGTTTGTCCCTTTTCCGGCTGCAGGTCATCTAGTTTCCGCCCTTGAGTTCGCGAAGCTTCTAATCAACCGTGATAACCGTCTCCGAGTAACTGTTTTAGTTATCAAATTCCCGCACACCACAGAAACCGATGTCTACACTAAGTCCCTTCCTATCTCCGATTCACTCCACATCATCAACCTTCCAGAATGTTCTCTTCCCCCAAACACCGATCCAGGTTCCTCTATGACTGCGCTCCTCGAAGCTCAGAAACCAAACATCAAACAGGCCGTATCTAACCTCACTACCAGATCAGAAAATGGAGTCCTTGCCGCTTTCGTTGTTGACATGTTCTGCACAAACATGATTGATGTTGCCAAAGAGTTCTCCGTGCCTACACTTGTCTTCTTCACTTCCGGTGTTGCGTTCCTTGGTTTGAGTTTTCATCTTTACACTCTTCGTCAACGAGACAATGTCTAGTCAACTAAGTTGTTACAACTCACTGACTTGGCCGTCCCAAGTTTCACTAACTTGGTTCCATCAAAAGCGTTGCCCAGTGCTGTGCTAAGTAAAGAATGGGAGTCGTTTATGATCGGCTATTGGAAAGGCCTCAAGAATGCTGATGGCTTTATAGTAAATTCATTTGAAGAGCTAGAATCCCATGCAGTTCACTCGTTTTCATCTGATCCTGGTTTAGCTGGTTTACCTATATATCCGGTAGGACCCATACTGAACCTGCAGCCCAAAACCAAGGGCACCGATGACATCATCAAGTGGCTTGATGATCAACCTCCTTCCTCGGTAGTTTTCCTCTGCTTCGGGAGTATGGGTTCTTTCAATGAGGATCAGGTTAAGCAGATTGCATGCGCTATTGAGAATAGTGGGGCCATGTTCATATGGTCTCTTCGTAAACCTCCACCAGAGGGCACCATGGCCGCGCCCTCTGATTACCCTCTTTTTGATTTGGGTTCAAGTTTACCCGAAGGATTTTTAGAGCGGACAGCAGAGATTGGAAGGGTCGTTGGATGGGCCCCACAAGTCCAAATACTAGCCCACCCAGCCATTGGAGGATTTGCTTCGCATTGTGGTTGGAATTCAGTACTTGAGAGCATTTATTTTGGTGTGCCTATAGCCACATGGCCGCTTTATGCAGAACAACAGACTAATGCTTTTGAATTGGTGTGTGAGTTGAAAATGGGAGTCGAAATTGCGTTGGATTATAGGGCGGAGTTTAATGGTGCGCCTAACTATCTTGTAACAGCCGATAAGATTGAGAGAGGAATAAGGAGTGTGTTGGATAAGGATGGAGATGTAAGGAAGAAAGTGAAAGAGATGAGTGAAAAAAGTAAGAAAACATTGTTAGAAGGTGGGTCTTCTTACGCTTATTTAGGTCGTTTGGTTGATTATATTATGAATCAGGTATGA
Protein sequence

>MS.gene038261.t1

MKKAEVVFVPFPAAGHLVSALEFAKLLINRDNRLRVTVLVIKFPHTTETDVYTKSLPISDSLHIINLPECSLPPNTDPGSSMTALLEAQKPNIKQAVSNLTTRSENGVLAAFVVDMFCTNMIDVAKEFSVPTLVFFTSGVAFLGLSFHLYTLPLPSAVLSKEWESFMIGYWKGLKNADGFIVNSFEELESHAVHSFSSDPGLAGLPIYPVGPILNLQPKTKGTDDIIKWLDDQPPSSVVFLCFGSMGSFNEDQVKQIACAIENSGAMFIWSLRKPPPEGTMAAPSDYPLFDLVEIALDYRAEFNGAPNYLVTADKIERGIRSVLDKDGDVRKKVKEMSEKSKKTLLEGGSSYAYLGRLVDYIMNQV