AlfalfaGEDB | MS.gene025918

M. sativa cultivar XinJiangDaYe / MS.gene025918

KEGG: K08237
GO: GO:0003674 GO:0003824 GO:0016740 GO:0016757 GO:0016758
PFAM: PF00201.21

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene025918.t1	XP_003626556.1	95	483	20	3	1	479	1	483	1.20E-262	915.2

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene025918.t1	Q9AR73	63.4	462	169	0	13	474	5	466	1.3e-175	617.5

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene025918.t1	G7L424	95.0	483	20	3	1	479	1	483	9.0e-263	915.2

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
MS.gene009405	MS.gene025918	PPI
MS.gene009637	MS.gene025918	PPI
MS.gene009890	MS.gene025918	PPI
MS.gene009895	MS.gene025918	PPI
MS.gene010467	MS.gene025918	PPI
MS.gene010469	MS.gene025918	PPI
MS.gene017041	MS.gene025918	PPI
MS.gene017042	MS.gene025918	PPI
MS.gene017043	MS.gene025918	PPI
MS.gene017044	MS.gene025918	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene025918.t1	MTR_7g117170	95.031	483	20	3	1	479	1	483	0.0	929
MS.gene025918.t1	MTR_8g027885	61.822	461	171	3	12	471	16	472	0.0	585
MS.gene025918.t1	MTR_8g027870	60.954	461	175	3	12	471	16	472	0.0	579
MS.gene025918.t1	MTR_8g009050	52.350	468	220	2	9	473	1	468	1.93e-179	511
MS.gene025918.t1	MTR_8g009055	51.923	468	222	2	9	473	1	468	4.28e-178	508
MS.gene025918.t1	MTR_8g008990	51.489	470	223	3	9	473	1	470	3.10e-172	493
MS.gene025918.t1	MTR_8g009063	51.282	468	225	2	9	473	8	475	3.66e-167	480
MS.gene025918.t1	MTR_8g009073	50.000	468	229	4	9	473	1	466	3.78e-167	480
MS.gene025918.t1	MTR_1g019510	49.356	466	234	2	9	473	1	465	5.77e-165	474
MS.gene025918.t1	MTR_0184s0040	48.941	472	231	5	8	473	15	482	8.87e-164	472

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene025918.t1	AT4G01070	62.099	467	169	5	10	471	4	467	0.0	590
MS.gene025918.t1	AT1G01420	57.234	470	196	4	13	479	10	477	0.0	547
MS.gene025918.t1	AT1G01420	57.234	470	196	4	13	479	7	474	0.0	547
MS.gene025918.t1	AT1G01390	54.894	470	208	3	13	479	24	492	0.0	524
MS.gene025918.t1	AT1G01390	54.894	470	208	3	13	479	7	475	0.0	523
MS.gene025918.t1	AT4G01070	60.057	348	129	6	10	352	4	346	4.30e-141	409
MS.gene025918.t1	AT3G50740	41.277	470	257	10	13	468	6	470	1.46e-111	338
MS.gene025918.t1	AT5G66690	39.565	460	265	7	13	465	6	459	1.27e-108	331
MS.gene025918.t1	AT5G26310	38.043	460	272	7	13	465	6	459	1.49e-105	323
MS.gene025918.t1	AT2G18570	36.326	479	279	12	13	478	4	469	2.65e-100	309

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Find 128 sgRNAs with CRISPR-Local

Find 160 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACCCACCAGTTGAGCCA+TGG	0.543571	7.1:+988296	None:intergenic
AAAAGTGGCCAAACCACTAA+AGG	0.518321	7.1:+988223	None:intergenic
AAACCATTCTCACCAACATT+TGG	0.408050	7.1:+988142	None:intergenic
AAACCCACCAGTTGAGCCAT+GGG	0.561495	7.1:+988297	None:intergenic
AAAGCTTCGTTATCAAATGA+AGG	0.510389	7.1:-988059	MS.gene025918:CDS
AAAGTATGTGAAATGGACTT+TGG	0.416845	7.1:+989174	None:intergenic
AAATGTTGGTGAGAATGGTT+TGG	0.502614	7.1:-988140	MS.gene025918:CDS
AACAGGGAAGTAGGTCAAAT+TGG	0.322029	7.1:-988625	MS.gene025918:CDS
AAGAGATACAGAGAAGCCGA+TGG	0.629191	7.1:-988751	MS.gene025918:CDS
AAGGAAAAGAGAGAGAGCCA+TGG	0.679106	7.1:+988930	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAGCTTCGTTATCAAATGA+AGG	-	chr7.1:989262-989281	MS.gene025918:CDS	30.0%
AAGGGTAGAAGACATATTTA+GGG	+	chr7.1:988365-988384	None:intergenic	30.0%
CAATTATCTGTTTACTAGAG+AGG	+	chr7.1:988803-988822	None:intergenic	30.0%
CCATTTGGTAGAAAATCAAA+AGG	+	chr7.1:988936-988955	None:intergenic	30.0%
GCATTAGCAACTTTATCATT+TGG	+	chr7.1:988882-988901	None:intergenic	30.0%
TAAATTGGAGATATGGTTTG+TGG	+	chr7.1:989333-989352	None:intergenic	30.0%
AAACCATTCTCACCAACATT+TGG	+	chr7.1:989182-989201	None:intergenic	35.0%
ACCATTATCAAAACCACCTA+TGG	-	chr7.1:987972-987991	MS.gene025918:CDS	35.0%
ACTGTTCTCTATAAGTCCAT+CGG	+	chr7.1:988589-988608	None:intergenic	35.0%
CAATTCAAACTCTCTGAGTT+CGG	+	chr7.1:988723-988742	None:intergenic	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr7.1	gene	987952	989391	987952	ID=MS.gene025918
chr7.1	mRNA	987952	989391	987952	ID=MS.gene025918.t1;Parent=MS.gene025918
chr7.1	exon	987952	989391	987952	ID=MS.gene025918.t1.exon1;Parent=MS.gene025918.t1
chr7.1	CDS	987952	989391	987952	ID=cds.MS.gene025918.t1;Parent=MS.gene025918.t1

Gene Sequence

>MS.gene025918

ATGGAAATCTCAAAACAAGAACCATTATCAAAACCACCTATGGTTGTGATGCTACCATCACCAGGCATGGGACATCTTATACCAATGATTGAATTCGCCAAAAGAATTATTCTTCATCAAAATCTTCAAATCACCTTCTTCATTCCCAGTGATGGTCCACCTTCAAAAGCCCAAAAAACCGTTCTTCAATCTCTTCCAAAGTCCATTTCACATACTTTCCTTCCACCAGTTTCCTTCTCAGACCTTCCACCAACCTCTGGGATAGAGACAATCATCTCCCTCACTGTCCTCCGTTCTCTCCCTTCTCTCCGTCAAAACTTCAACACTCTCTCTGATACTCATACTATCACTGCTGTTGTCGTCGACCTCTTTGGGACAGACGCTTTTGACGTCGCTGGAGAATTCAACGTCCCTAAATATGTCTTCTACCCTTCAACTGCCATGGCTCTCTCTCTTTTCCTTTACCTTCCTCGCCTTGACCAGGAAGTTCACTGCGAGTTTAGAGAATTGACTGAACCGGTGAAAATTCCTGGTTGTATTCCGATTCATGGTAAATATTTACTAGACCCGCTTCAAGACAGGAAGAACGATGCTTACCAAAGTGTCCTTCGCAACGGGAAGAGATACAGAGAAGCCGATGGACTTATAGAGAACAGTTTCCTTGAACTTGAACCAGGTCCAATCAAGGAGTTACTAAAGGAAGAACCGGGAAAACCTAAGTTTTACCCGGTCGGACCATTAGTAAACAGGGAAGTAGGTCAAATTGGACCGAACTCAGAGAGTTTGAATTGGTTAGATAATCAACCACACGGGAGTGTTCTGTTTGTTTCTTTTGGAAGTGGTGGGACCCTCTCTAGTAAACAGATAATTGAATTGGCACTTGGCTTGGAAATGAGTGAGCAACGGTTTTTATGGGTTGTTAGAAGTCCAAATGATAAAGTTGCTAATGCTTCTTACTTCAGTGCTGAAACACATTCTGATCCTTTTGATTTTCTACCAAATGGGTTTTTGGAGAGAACCAAAGGGAGAGGTCTTGTTGTGTCATCTTGGGCCCCACAACCACAGGTTTTGGCCCATGGCTCAACTGGTGGGTTTTTGACTCATTGTGGTTGGAATTCAGTTCTTGAGAGTGTTGTTAATGGGGTGCCTTTAGTGGTTTGGCCACTTTTTGCAGAGCAAAAGATGAATGCTGTTATTTTAACTGAGGATGTTAAGGTTGGGTTGAGACCAAATGTTGGTGAGAATGGTTTGGTTGAGAGATTGGAAATTGCTAGTGTTGTTAAGTGTCTTATGGAAGGTGAAGAAGGGAAAAAGCTTCGTTATCAAATGAAGGATCTTAAGGATGCAGCTGCTAAAACTCTTGGTGAGAATGGAACTTCCACAAACCATATCTCCAATTTAGCTCTCAAGTGGAGCAACAAATCCACTGTAATAAATTAA

Protein sequence

>MS.gene025918.t1

MEISKQEPLSKPPMVVMLPSPGMGHLIPMIEFAKRIILHQNLQITFFIPSDGPPSKAQKTVLQSLPKSISHTFLPPVSFSDLPPTSGIETIISLTVLRSLPSLRQNFNTLSDTHTITAVVVDLFGTDAFDVAGEFNVPKYVFYPSTAMALSLFLYLPRLDQEVHCEFRELTEPVKIPGCIPIHGKYLLDPLQDRKNDAYQSVLRNGKRYREADGLIENSFLELEPGPIKELLKEEPGKPKFYPVGPLVNREVGQIGPNSESLNWLDNQPHGSVLFVSFGSGGTLSSKQIIELALGLEMSEQRFLWVVRSPNDKVANASYFSAETHSDPFDFLPNGFLERTKGRGLVVSSWAPQPQVLAHGSTGGFLTHCGWNSVLESVVNGVPLVVWPLFAEQKMNAVILTEDVKVGLRPNVGENGLVERLEIASVVKCLMEGEEGKKLRYQMKDLKDAAAKTLGENGTSTNHISNLALKWSNKSTVIN