AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene44888

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene44888.t1 MTR_5g070090 94.839 310 16 0 1 310 1 310 0.0 606
MS.gene44888.t1 MTR_5g070090 83.505 97 15 1 290 385 369 465 5.14e-42 153
MS.gene44888.t1 MTR_5g070040 72.327 318 80 3 1 315 1 313 1.71e-161 461
MS.gene44888.t1 MTR_5g070040 57.143 98 37 3 290 385 376 470 1.00e-20 94.0
MS.gene44888.t1 MTR_5g090700 40.317 315 165 11 8 310 2 305 1.70e-61 205
MS.gene44888.t1 MTR_5g090600 39.936 313 163 9 8 310 2 299 6.15e-60 201
MS.gene44888.t1 MTR_2g040530 36.688 308 180 6 9 310 3 301 1.77e-59 200
MS.gene44888.t1 MTR_2g040530 32.967 91 61 0 292 382 375 465 9.60e-12 66.6
MS.gene44888.t1 MTR_5g090630 40.523 306 166 9 8 310 2 294 1.34e-58 197
MS.gene44888.t1 MTR_2g046750 35.737 319 177 9 9 315 3 305 1.55e-57 194
MS.gene44888.t1 MTR_2g046750 34.066 91 60 0 292 382 376 466 1.36e-11 66.2
MS.gene44888.t1 MTR_5g098930 38.801 317 158 11 8 310 2 296 2.65e-57 194
MS.gene44888.t1 MTR_2g046820 37.618 319 160 11 9 310 3 299 8.57e-57 192
MS.gene44888.t1 MTR_5g090670 39.683 315 167 10 8 310 2 305 1.57e-56 192
MS.gene44888.t1 MTR_5g090620 36.937 333 185 11 8 331 2 318 4.27e-56 191
MS.gene44888.t1 MTR_5g090660 38.907 311 171 10 8 310 2 301 1.82e-55 191
MS.gene44888.t1 MTR_5g090610 38.387 310 162 10 8 310 2 289 8.93e-55 187
MS.gene44888.t1 MTR_5g090580 37.500 312 176 9 8 310 2 303 9.72e-54 185
MS.gene44888.t1 MTR_3g009510 32.166 314 194 10 8 310 2 307 4.61e-41 151
MS.gene44888.t1 MTR_5g090650 33.333 315 160 11 8 315 2 273 4.73e-41 147
MS.gene44888.t1 MTR_6g488280 30.284 317 190 11 6 310 1 298 5.18e-34 131
MS.gene44888.t1 MTR_0428s0010 30.284 317 190 11 6 310 1 298 5.18e-34 131
MS.gene44888.t1 MTR_6g023665 30.625 320 201 10 2 315 4 308 5.13e-33 129
MS.gene44888.t1 MTR_8g006730 28.616 318 204 13 6 315 1 303 6.08e-32 125
MS.gene44888.t1 MTR_8g027885 35.410 305 169 15 14 310 21 305 7.90e-32 125
MS.gene44888.t1 MTR_7g117170 34.194 310 176 13 12 310 16 308 2.98e-31 124
MS.gene44888.t1 MTR_8g006710 28.793 323 208 12 6 321 1 308 5.81e-31 123
MS.gene44888.t1 MTR_6g033675 27.660 376 230 13 9 364 2 355 3.52e-30 121
MS.gene44888.t1 MTR_8g027870 32.237 304 180 12 14 310 21 305 1.45e-29 119
MS.gene44888.t1 MTR_8g006220 31.230 317 186 14 6 310 1 297 1.50e-27 114
MS.gene44888.t1 MTR_8g074550 28.076 317 205 10 4 310 1 304 1.54e-27 114
MS.gene44888.t1 MTR_8g465670 32.177 317 179 17 8 310 4 298 8.17e-27 111
MS.gene44888.t1 MTR_5g019580 28.750 320 199 13 6 310 2 307 1.66e-25 108
MS.gene44888.t1 MTR_6g033725 28.076 317 199 10 9 310 2 304 2.33e-24 104
MS.gene44888.t1 MTR_6g033615 29.595 321 178 11 9 310 2 293 3.36e-24 103
MS.gene44888.t1 MTR_6g033625 28.354 328 187 11 9 310 2 307 3.50e-24 103
MS.gene44888.t1 MTR_6g005600 29.967 307 191 14 9 306 4 295 8.12e-24 102
MS.gene44888.t1 MTR_0184s0040 28.406 345 218 13 4 341 14 336 1.84e-23 102
MS.gene44888.t1 MTR_0078s0190 28.406 345 218 13 4 341 14 336 1.84e-23 102
MS.gene44888.t1 MTR_0078s0010 27.405 343 216 13 8 341 3 321 4.21e-23 100
MS.gene44888.t1 MTR_6g033660 27.760 317 196 9 12 310 5 306 5.12e-23 100
MS.gene44888.t1 MTR_8g009055 27.036 307 214 7 6 310 1 299 1.17e-21 96.7
MS.gene44888.t1 MTR_4g082820 27.606 355 226 15 16 352 12 353 2.71e-21 95.5
MS.gene44888.t1 MTR_8g009050 27.597 308 201 11 6 305 1 294 7.56e-21 94.4
MS.gene44888.t1 MTR_8g006270 29.290 338 198 17 6 332 1 308 9.38e-21 94.0
MS.gene44888.t1 MTR_8g009063 27.564 312 206 11 6 310 8 306 1.17e-20 93.6
MS.gene44888.t1 MTR_3g009520 26.054 261 179 6 6 254 1 259 1.65e-20 90.5
MS.gene44888.t1 MTR_7g047030 27.885 312 202 10 8 310 3 300 1.95e-20 93.2
MS.gene44888.t1 MTR_7g047270 27.885 312 202 10 8 310 3 300 1.95e-20 93.2
MS.gene44888.t1 MTR_8g009073 28.939 311 203 11 6 310 1 299 2.02e-20 92.8
MS.gene44888.t1 MTR_0078s0200 28.247 308 193 13 15 310 10 301 6.73e-20 89.7
MS.gene44888.t1 MTR_0184s0030 27.687 307 196 12 15 310 10 301 8.47e-20 91.3
MS.gene44888.t1 MTR_8g008990 26.797 306 214 7 6 309 1 298 1.02e-19 90.9
MS.gene44888.t1 MTR_0078s0060 27.273 341 218 13 8 341 3 320 1.31e-19 90.5
MS.gene44888.t1 MTR_8g006230 29.469 207 132 7 112 315 49 244 2.00e-19 87.8
MS.gene44888.t1 MTR_3g009500 25.681 257 167 9 9 248 3 252 2.96e-19 87.0
MS.gene44888.t1 MTR_7g046490 27.532 316 198 12 6 310 1 296 9.94e-19 87.8
MS.gene44888.t1 MTR_8g077590 26.316 323 202 15 3 310 1 302 1.50e-18 87.4
MS.gene44888.t1 MTR_5g072810 28.391 317 189 13 7 308 8 301 3.08e-18 86.3
MS.gene44888.t1 MTR_1g019510 26.452 310 210 10 6 310 1 297 1.55e-17 84.3
MS.gene44888.t1 MTR_8g008970 26.861 309 212 9 6 310 1 299 8.51e-17 82.8
MS.gene44888.t1 MTR_8g009067 27.302 315 203 11 6 310 1 299 2.33e-16 80.9
MS.gene44888.t1 MTR_5g072840 26.837 313 192 14 11 310 10 298 1.99e-15 77.8
MS.gene44888.t1 MTR_4g082810 26.190 378 218 14 16 364 12 357 2.38e-15 77.4
MS.gene44888.t1 MTR_5g045970 24.179 335 204 11 12 314 17 333 1.39e-14 75.5
MS.gene44888.t1 MTR_8g012635 24.920 313 212 12 8 310 3 302 2.07e-14 74.7
MS.gene44888.t1 MTR_5g031830 28.620 297 178 13 12 285 14 299 4.04e-14 73.9
MS.gene44888.t1 MTR_8g066610 26.332 319 206 14 12 310 10 319 4.40e-14 73.9
MS.gene44888.t1 MTR_6g038200 25.478 314 200 13 7 305 7 301 1.03e-13 72.8
MS.gene44888.t1 MTR_7g117405 24.933 373 209 18 5 345 6 339 2.61e-13 71.6
MS.gene44888.t1 MTR_2g098430 25.077 323 206 13 8 310 3 309 3.24e-13 71.2
MS.gene44888.t1 MTR_7g102520 24.540 326 192 14 12 305 13 316 3.71e-13 71.2
MS.gene44888.t1 MTR_6g014250 23.967 363 235 12 6 341 5 353 6.08e-13 70.5
MS.gene44888.t1 MTR_8g012745 23.377 308 223 9 8 310 3 302 8.62e-13 70.1
MS.gene44888.t1 MTR_3g479470 23.724 333 216 11 1 305 3 325 1.08e-12 69.7
MS.gene44888.t1 MTR_5g464810 28.070 342 190 18 1 305 1 323 3.99e-12 67.8
MS.gene44888.t1 MTR_5g064240 28.070 342 190 18 1 305 1 323 3.99e-12 67.8
MS.gene44888.t1 MTR_6g038220 26.101 318 192 15 7 305 7 300 7.78e-12 67.0
MS.gene44888.t1 MTR_5g464760 26.216 370 226 17 2 341 3 355 7.94e-12 67.0
MS.gene44888.t1 MTR_3g008010 25.439 342 190 14 6 305 5 323 1.16e-11 66.2
MS.gene44888.t1 MTR_1g062380 23.867 331 211 14 12 314 17 334 2.02e-11 65.9
MS.gene44888.t1 MTR_5g031840 26.000 350 222 14 12 339 7 341 2.69e-11 65.1
MS.gene44888.t1 MTR_6g014020 24.033 362 234 13 8 341 7 355 7.84e-11 63.9
MS.gene44888.t1 MTR_5g035580 23.136 389 219 14 5 364 7 344 9.92e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene44888.t1 AT2G29730 39.011 364 200 7 8 364 2 350 4.46e-77 246
MS.gene44888.t1 AT1G07240 41.945 329 171 10 8 322 2 324 2.32e-75 241
MS.gene44888.t1 AT2G29740 41.049 324 169 11 7 315 4 320 3.50e-75 241
MS.gene44888.t1 AT1G07260 41.433 321 173 8 6 315 1 317 4.71e-75 241
MS.gene44888.t1 AT1G07260 39.744 78 46 1 309 385 398 475 2.98e-12 68.2
MS.gene44888.t1 AT1G07250 40.994 322 170 11 8 315 3 318 1.35e-73 237
MS.gene44888.t1 AT2G29710 38.567 363 203 6 8 364 2 350 1.17e-70 229
MS.gene44888.t1 AT2G29750 40.385 312 166 10 7 303 4 310 1.15e-66 219
MS.gene44888.t1 AT3G21790 39.432 317 167 9 9 310 2 308 1.75e-59 201
MS.gene44888.t1 AT3G21780 39.744 312 164 11 9 310 2 299 3.63e-57 194
MS.gene44888.t1 AT3G21760 38.170 317 174 9 9 310 2 311 1.10e-56 193
MS.gene44888.t1 AT3G21750 33.866 313 179 9 9 310 2 297 9.43e-52 179
MS.gene44888.t1 AT3G21800 35.647 317 175 9 9 310 3 305 4.63e-48 170
MS.gene44888.t1 AT4G15280 35.048 311 185 9 9 310 2 304 1.62e-46 166
MS.gene44888.t1 AT4G15280 35.048 311 185 9 9 310 34 336 2.84e-46 166
MS.gene44888.t1 AT4G15260 39.583 192 105 6 122 310 1 184 7.28e-35 132
MS.gene44888.t1 AT3G16520 32.903 310 193 12 12 315 6 306 3.57e-31 123
MS.gene44888.t1 AT3G16520 32.903 310 193 12 12 315 6 306 3.78e-31 123
MS.gene44888.t1 AT3G16520 32.787 305 192 11 12 310 6 303 3.94e-31 123
MS.gene44888.t1 AT4G01070 31.210 314 196 9 4 310 1 301 5.11e-29 115
MS.gene44888.t1 AT4G01070 31.210 314 196 9 4 310 1 301 7.10e-29 117
MS.gene44888.t1 AT4G15270 34.272 213 125 4 9 210 2 210 2.71e-27 108
MS.gene44888.t1 AT4G15270 32.314 229 137 6 9 225 2 224 3.50e-26 107
MS.gene44888.t1 AT1G01390 30.293 307 194 9 11 310 25 318 1.28e-25 108
MS.gene44888.t1 AT1G01390 30.293 307 194 9 11 310 8 301 1.55e-25 108
MS.gene44888.t1 AT4G15270 33.180 217 127 6 9 213 2 212 3.23e-25 102
MS.gene44888.t1 AT1G01420 32.039 309 178 12 15 310 12 301 7.09e-25 106
MS.gene44888.t1 AT1G01420 32.039 309 178 12 15 310 15 304 7.95e-25 105
MS.gene44888.t1 AT3G50740 26.129 310 208 11 9 310 5 301 4.70e-22 97.8
MS.gene44888.t1 AT2G18570 25.161 310 204 12 13 310 7 300 8.56e-22 97.1
MS.gene44888.t1 AT5G66690 25.000 312 204 12 9 310 5 296 1.77e-19 90.1
MS.gene44888.t1 AT4G36770 26.710 307 198 11 14 310 7 296 6.79e-19 88.6
MS.gene44888.t1 AT5G26310 25.397 315 199 14 9 310 5 296 4.23e-18 86.3
MS.gene44888.t1 AT2G30150 25.460 326 178 16 12 309 14 302 4.28e-15 77.0
MS.gene44888.t1 AT2G30150 25.697 323 175 16 15 309 1 286 5.65e-15 76.6
MS.gene44888.t1 AT1G73880 25.378 331 221 12 9 327 12 328 1.54e-14 75.5
MS.gene44888.t1 AT1G22360 24.924 329 209 13 6 306 5 323 2.22e-14 74.7
MS.gene44888.t1 AT1G22360 24.924 329 209 13 6 306 5 323 3.09e-14 74.3
MS.gene44888.t1 AT1G22400 25.444 338 203 14 3 306 5 327 2.01e-13 72.0
MS.gene44888.t1 AT5G14860 26.113 337 202 16 4 310 1 320 2.92e-13 71.6
MS.gene44888.t1 AT1G10400 23.861 373 224 16 8 352 4 344 3.56e-13 71.2
MS.gene44888.t1 AT2G36800 23.602 322 215 13 5 305 4 315 1.49e-12 69.3
MS.gene44888.t1 AT1G22370 25.392 319 203 12 8 302 10 317 1.04e-11 66.6
MS.gene44888.t1 AT5G38010 25.407 307 207 11 7 305 6 298 1.17e-11 66.2
MS.gene44888.t1 AT2G30140 24.762 315 205 14 7 310 9 302 6.56e-11 63.9
MS.gene44888.t1 AT4G34138 25.000 360 227 13 4 341 5 343 8.50e-11 63.9
MS.gene44888.t1 AT5G12890 26.168 321 192 14 11 305 10 311 9.37e-11 63.5

Find 68 sgRNAs with CRISPR-Local

Find 121 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGGAATGGGGTGTAGGTTT+GGG 0.176366 5.2:+59345391 MS.gene44888:CDS
TGCTGAGCTTGATCCAATTT+AGG 0.192689 5.2:-59344986 None:intergenic
ATTGATGTTGGAAATGAATT+TGG 0.238848 5.2:+59344602 MS.gene44888:CDS
GAAGGAATGGGGTGTAGGTT+TGG 0.254892 5.2:+59345390 MS.gene44888:CDS
GCTGAGCTTGATCCAATTTA+GGG 0.255292 5.2:-59344985 None:intergenic
GCATCAGGTAAAACATTAAA+AGG 0.305297 5.2:-59344770 None:intergenic
AGGTCAACCTAACCCTAAAT+TGG 0.307191 5.2:+59344973 MS.gene44888:CDS
TGTGGAGGTGGTTCTACTTC+AGG 0.310331 5.2:-59344443 None:intergenic
AAAGTCAAGATGTAAAATTC+TGG 0.325907 5.2:-59344482 None:intergenic
AAACTTATTGATGATATTAC+AGG 0.328977 5.2:+59345586 MS.gene44888:CDS
GAAAGGATTGAAGGATTTGA+TGG 0.340181 5.2:+59345468 MS.gene44888:CDS
GGCTGAGAGTGGACTATAGA+AGG 0.347835 5.2:+59345413 MS.gene44888:CDS
GTCTTCTGCATCAAGTTTCC+AGG 0.360293 5.2:+59344350 MS.gene44888:CDS
CATCGAAAACATCTTCGATT+TGG 0.361596 5.2:-59344694 None:intergenic
CATCAGTTGTTGAATATTCC+TGG 0.366179 5.2:+59344731 MS.gene44888:CDS
TTCATTCCTGCACCAGGAAT+TGG 0.371292 5.2:+59344260 MS.gene44888:CDS
GCTTGATCCAATTTAGGGTT+AGG 0.382679 5.2:-59344980 None:intergenic
GGAAGCATGGGAGTTAGCTT+TGG 0.385426 5.2:+59345070 MS.gene44888:CDS
GAAGCTAAGTGGCCAATTCC+TGG 0.388433 5.2:-59344272 None:intergenic
GACTTAAGCATAGTGGAGTT+AGG 0.398532 5.2:+59345122 MS.gene44888:CDS
CAAACAAGAGAAATAGCATT+AGG 0.401684 5.2:+59345100 MS.gene44888:CDS
AGCATGATCTTATATTGAAA+TGG 0.416804 5.2:+59345005 MS.gene44888:CDS
TTGAAACACAAAAGAAATCT+AGG 0.416883 5.2:-59344577 None:intergenic
TTTAGTAGCTCTTGTGGAGG+TGG 0.421818 5.2:-59344455 None:intergenic
GGTATTAACAATAATCCCTT+TGG 0.424481 5.2:-59344855 None:intergenic
TCTAGGAATGCTGTTGTTGA+TGG 0.443758 5.2:+59345538 MS.gene44888:CDS
TTTAGGTTGGTGAAGGAATG+GGG 0.457761 5.2:+59345379 MS.gene44888:intron
CGAAAACATCTTCGATTTGG+CGG 0.459719 5.2:-59344691 None:intergenic
GAATCTGCAAAGGGTGTGCC+TGG 0.461256 5.2:-59344368 None:intergenic
TGTAGGTTTGGGGCTGAGAG+TGG 0.465531 5.2:+59345402 MS.gene44888:CDS
AGGAATGGGGTGTAGGTTTG+GGG 0.479020 5.2:+59345392 MS.gene44888:CDS
GGAGATTGAGAAAGGATTGA+AGG 0.482769 5.2:+59345459 MS.gene44888:CDS
AAAGGACCAACAGCATAGAT+AGG 0.483097 5.2:-59344938 None:intergenic
TTGGTGAAGGAATGGGGTGT+AGG 0.491795 5.2:+59345385 MS.gene44888:CDS
GGATCTTCTTTAATTTCTGT+CGG 0.492776 5.2:+59345562 MS.gene44888:CDS
TGCTATTTCTCTTGTTTGAG+AGG 0.500007 5.2:-59345095 None:intergenic
CTCATCTTCATTCCTGCACC+AGG 0.502272 5.2:+59344254 MS.gene44888:CDS
GCTATTTCTCTTGTTTGAGA+GGG 0.503078 5.2:-59345094 None:intergenic
TCTTTATTAAAACAAGCATC+AGG 0.510179 5.2:-59344785 None:intergenic
AAGAGATCAAAGAGATGTCT+AGG 0.510224 5.2:+59345521 MS.gene44888:CDS
TAGTGATGTTGTAGCGGCCG+AGG 0.515521 5.2:+59345438 MS.gene44888:CDS
TGTGTTTCAATGATTGATGT+TGG 0.520106 5.2:+59344590 MS.gene44888:CDS
CAATCCTTTCTCAATCTCCT+CGG 0.520210 5.2:-59345455 None:intergenic
TTGATGGTTGCTTTGACATG+AGG 0.524267 5.2:-59344521 None:intergenic
AAGTGGCCAATTCCTGGTGC+AGG 0.524765 5.2:-59344266 None:intergenic
ATTCCTCCTATCTATGCTGT+TGG 0.528901 5.2:+59344932 MS.gene44888:CDS
GGAACTTGATTTGAGATACC+AGG 0.534218 5.2:-59344749 None:intergenic
AGATTGTTCCAAATCTGAAA+AGG 0.546266 5.2:-59344876 None:intergenic
AGGAATGCTGTTGTTGATGG+TGG 0.550773 5.2:+59345541 MS.gene44888:CDS
AGATCAATGAGTTGAATTTG+TGG 0.552600 5.2:-59344419 None:intergenic
GCATTAGGACTTAAGCATAG+TGG 0.558018 5.2:+59345115 MS.gene44888:CDS
GGTCCTTTGTTAGATCTCAA+AGG 0.560078 5.2:+59344953 MS.gene44888:CDS
AGATAGCATTGTGCACAAGA+AGG 0.570738 5.2:+59345495 MS.gene44888:CDS
GCGGCCGAGGAGATTGAGAA+AGG 0.584169 5.2:+59345451 MS.gene44888:CDS
TTGATATATGAATCTGCAAA+GGG 0.584607 5.2:-59344377 None:intergenic
ATTCAAGAGCTGAAGCTAAG+TGG 0.586238 5.2:-59344283 None:intergenic
GCTGAGAGTGGACTATAGAA+GGG 0.592272 5.2:+59345414 MS.gene44888:CDS
GGACCAACAGCATAGATAGG+AGG 0.593467 5.2:-59344935 None:intergenic
AGAGGTTTAGAGACACCAAA+GGG 0.612890 5.2:+59344840 MS.gene44888:CDS
GAGAGGTTTAGAGACACCAA+AGG 0.619389 5.2:+59344839 MS.gene44888:CDS
GAGTTGAATTTGTGGTTGTG+AGG 0.630924 5.2:-59344411 None:intergenic
CTTATTATAAACTAGCTGAG+AGG 0.640289 5.2:+59344822 MS.gene44888:CDS
CAACAACTGATGATCACGGT+CGG 0.648446 5.2:-59344720 None:intergenic
AAGGGGTAGTGATGTTGTAG+CGG 0.652755 5.2:+59345432 MS.gene44888:CDS
TATTCAACAACTGATGATCA+CGG 0.656139 5.2:-59344724 None:intergenic
TTTGATATATGAATCTGCAA+AGG 0.661894 5.2:-59344378 None:intergenic
CTGAGAGTGGACTATAGAAG+GGG 0.670408 5.2:+59345415 MS.gene44888:CDS
TGACCTTTGAGATCTAACAA+AGG 0.729571 5.2:-59344956 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATGTTAAAAACAAATAAGAA+GGG - chr5.2:59344631-59344650 None:intergenic 15.0%
!!! AACAATTTTGTTTGATAAAA+TGG - chr5.2:59344549-59344568 None:intergenic 15.0%
!!! ATTTTATCAAACAAAATTGT+TGG + chr5.2:59344548-59344567 MS.gene44888:CDS 15.0%
!!! TTTTATCAAACAAAATTGTT+GGG + chr5.2:59344549-59344568 MS.gene44888:CDS 15.0%
!! GATGTTAAAAACAAATAAGA+AGG - chr5.2:59344632-59344651 None:intergenic 20.0%
!!! AAACTTATTGATGATATTAC+AGG + chr5.2:59345586-59345605 MS.gene44888:CDS 20.0%
!!! ATTTTACATCTTGACTTTTT+TGG + chr5.2:59344487-59344506 MS.gene44888:CDS 20.0%
!!! TTGTTTTTAACATCAAATGT+TGG + chr5.2:59344638-59344657 MS.gene44888:CDS 20.0%
! AAAGTCAAGATGTAAAATTC+TGG - chr5.2:59344485-59344504 None:intergenic 25.0%
! AGCATGATCTTATATTGAAA+TGG + chr5.2:59345005-59345024 MS.gene44888:CDS 25.0%
! ATTGATGTTGGAAATGAATT+TGG + chr5.2:59344602-59344621 MS.gene44888:CDS 25.0%
! TCTTTATTAAAACAAGCATC+AGG - chr5.2:59344788-59344807 None:intergenic 25.0%
! TTGAAACACAAAAGAAATCT+AGG - chr5.2:59344580-59344599 None:intergenic 25.0%
! TTGATATATGAATCTGCAAA+GGG - chr5.2:59344380-59344399 None:intergenic 25.0%
! TTTGATATATGAATCTGCAA+AGG - chr5.2:59344381-59344400 None:intergenic 25.0%
!! AGAAAAACAACTGATTTATC+TGG - chr5.2:59345043-59345062 None:intergenic 25.0%
!! TGTTAATACCTTTTCAGATT+TGG + chr5.2:59344868-59344887 MS.gene44888:CDS 25.0%
!!! ATTCTATTTTGGAAAGTATG+TGG + chr5.2:59345302-59345321 MS.gene44888:intron 25.0%
!!! GATGCTTGTTTTAATAAAGA+TGG + chr5.2:59344788-59344807 MS.gene44888:CDS 25.0%
!!! GTTTGATAAAATGGTTTTGA+TGG - chr5.2:59344540-59344559 None:intergenic 25.0%
!!! TCAGTTGTTTTTCTATGTTT+TGG + chr5.2:59345049-59345068 MS.gene44888:CDS 25.0%
AGATTGTTCCAAATCTGAAA+AGG - chr5.2:59344879-59344898 None:intergenic 30.0%
CAAACAAGAGAAATAGCATT+AGG + chr5.2:59345100-59345119 MS.gene44888:CDS 30.0%
CTTATTATAAACTAGCTGAG+AGG + chr5.2:59344822-59344841 MS.gene44888:CDS 30.0%
GCATCAGGTAAAACATTAAA+AGG - chr5.2:59344773-59344792 None:intergenic 30.0%
GGTATTAACAATAATCCCTT+TGG - chr5.2:59344858-59344877 None:intergenic 30.0%
TATTCAACAACTGATGATCA+CGG - chr5.2:59344727-59344746 None:intergenic 30.0%
TGTGTTTCAATGATTGATGT+TGG + chr5.2:59344590-59344609 MS.gene44888:CDS 30.0%
TTAGAATGGATGGAATTAGA+AGG + chr5.2:59345193-59345212 MS.gene44888:intron 30.0%
! AACAACAGCTTAATGCTTTT+AGG + chr5.2:59345362-59345381 MS.gene44888:intron 30.0%
! AGATCAATGAGTTGAATTTG+TGG - chr5.2:59344422-59344441 None:intergenic 30.0%
! GGATCTTCTTTAATTTCTGT+CGG + chr5.2:59345562-59345581 MS.gene44888:CDS 30.0%
! TGATGCAAAGATTTTTGTCA+TGG - chr5.2:59344328-59344347 None:intergenic 30.0%
! TTGTGGATGGAATTCTATTT+TGG + chr5.2:59345291-59345310 MS.gene44888:intron 30.0%
!! AGAAAAAAGTGTTTCCAGAA+GGG + chr5.2:59345167-59345186 MS.gene44888:intron 30.0%
!! ATTTTGGAAAGTATGTGGTT+TGG + chr5.2:59345307-59345326 MS.gene44888:intron 30.0%
!!! GCTTGTTTTAATAAAGATGG+TGG + chr5.2:59344791-59344810 MS.gene44888:CDS 30.0%
!!! TTTCTATGTTTTGGAAGCAT+GGG + chr5.2:59345058-59345077 MS.gene44888:CDS 30.0%
!!! TTTTCTATGTTTTGGAAGCA+TGG + chr5.2:59345057-59345076 MS.gene44888:CDS 30.0%
AAGAGATCAAAGAGATGTCT+AGG + chr5.2:59345521-59345540 MS.gene44888:CDS 35.0%
ATGGATGGAATTAGAAGGTA+AGG + chr5.2:59345198-59345217 MS.gene44888:intron 35.0%
CATCAGTTGTTGAATATTCC+TGG + chr5.2:59344731-59344750 MS.gene44888:CDS 35.0%
CATCGAAAACATCTTCGATT+TGG - chr5.2:59344697-59344716 None:intergenic 35.0%
GAAAGGATTGAAGGATTTGA+TGG + chr5.2:59345468-59345487 MS.gene44888:CDS 35.0%
TAAGGGAATGATATGTGGAT+GGG + chr5.2:59345216-59345235 MS.gene44888:intron 35.0%
TAGATAGGCCATGTCAATAT+TGG - chr5.2:59345337-59345356 None:intergenic 35.0%
TGGATGGAATTAGAAGGTAA+GGG + chr5.2:59345199-59345218 MS.gene44888:intron 35.0%
TTGGTGTACCAATATTGACA+TGG + chr5.2:59345326-59345345 MS.gene44888:intron 35.0%
! ATCCATTCTAAAAACCCTTC+TGG - chr5.2:59345184-59345203 None:intergenic 35.0%
! GATTTGTTTCACATTGTGGA+TGG + chr5.2:59345278-59345297 MS.gene44888:intron 35.0%
! GCAAAGATTTTTGTCATGGT+TGG - chr5.2:59344324-59344343 None:intergenic 35.0%
!! ACAGCTTAATGCTTTTAGGT+TGG + chr5.2:59345366-59345385 MS.gene44888:intron 35.0%
!! GAGAAAAAAGTGTTTCCAGA+AGG + chr5.2:59345166-59345185 MS.gene44888:intron 35.0%
!! GCTATTTCTCTTGTTTGAGA+GGG - chr5.2:59345097-59345116 None:intergenic 35.0%
!! TGACCTTTGAGATCTAACAA+AGG - chr5.2:59344959-59344978 None:intergenic 35.0%
!! TGCTATTTCTCTTGTTTGAG+AGG - chr5.2:59345098-59345117 None:intergenic 35.0%
!! TTCCAGAAGGGTTTTTAGAA+TGG + chr5.2:59345179-59345198 MS.gene44888:intron 35.0%
!!! AGAAGGGTTTTTAGAATGGA+TGG + chr5.2:59345183-59345202 MS.gene44888:intron 35.0%
!!! TAATGCTTTTAGGTTGGTGA+AGG + chr5.2:59345372-59345391 MS.gene44888:intron 35.0%
!!! TTCGATTTGGCGGTTTTTAA+GGG - chr5.2:59344684-59344703 None:intergenic 35.0%
!!! TTTTAGGTTGGTGAAGGAAT+GGG + chr5.2:59345378-59345397 MS.gene44888:intron 35.0%
AAAGGACCAACAGCATAGAT+AGG - chr5.2:59344941-59344960 None:intergenic 40.0%
AGAGGTTTAGAGACACCAAA+GGG + chr5.2:59344840-59344859 MS.gene44888:CDS 40.0%
AGATAGCATTGTGCACAAGA+AGG + chr5.2:59345495-59345514 MS.gene44888:CDS 40.0%
AGCTGTTGTTCTGCATAGAT+AGG - chr5.2:59345352-59345371 None:intergenic 40.0%
AGGTCAACCTAACCCTAAAT+TGG + chr5.2:59344973-59344992 MS.gene44888:CDS 40.0%
ATTCAAGAGCTGAAGCTAAG+TGG - chr5.2:59344286-59344305 None:intergenic 40.0%
ATTCCTCCTATCTATGCTGT+TGG + chr5.2:59344932-59344951 MS.gene44888:CDS 40.0%
CAATCCTTTCTCAATCTCCT+CGG - chr5.2:59345458-59345477 None:intergenic 40.0%
GAAGGTAAGGGAATGATATG+TGG + chr5.2:59345211-59345230 MS.gene44888:intron 40.0%
GAGTTGAATTTGTGGTTGTG+AGG - chr5.2:59344414-59344433 None:intergenic 40.0%
GCATTAGGACTTAAGCATAG+TGG + chr5.2:59345115-59345134 MS.gene44888:CDS 40.0%
GCTGAGCTTGATCCAATTTA+GGG - chr5.2:59344988-59345007 None:intergenic 40.0%
GGAGATTGAGAAAGGATTGA+AGG + chr5.2:59345459-59345478 MS.gene44888:CDS 40.0%
GGTCCTTTGTTAGATCTCAA+AGG + chr5.2:59344953-59344972 MS.gene44888:CDS 40.0%
GTAAGGGAATGATATGTGGA+TGG + chr5.2:59345215-59345234 MS.gene44888:intron 40.0%
TCTAGGAATGCTGTTGTTGA+TGG + chr5.2:59345538-59345557 MS.gene44888:CDS 40.0%
TGCTGAGCTTGATCCAATTT+AGG - chr5.2:59344989-59345008 None:intergenic 40.0%
! ATAGTGGAGTTAGGTTCTTG+TGG + chr5.2:59345131-59345150 MS.gene44888:intron 40.0%
! GACTTAAGCATAGTGGAGTT+AGG + chr5.2:59345122-59345141 MS.gene44888:CDS 40.0%
! GCTTGATCCAATTTAGGGTT+AGG - chr5.2:59344983-59345002 None:intergenic 40.0%
! GGAACTTGATTTGAGATACC+AGG - chr5.2:59344752-59344771 None:intergenic 40.0%
! GGTGGATTTGTTTCACATTG+TGG + chr5.2:59345274-59345293 MS.gene44888:intron 40.0%
! TTGATGGTTGCTTTGACATG+AGG - chr5.2:59344524-59344543 None:intergenic 40.0%
!! CGAAAACATCTTCGATTTGG+CGG - chr5.2:59344694-59344713 None:intergenic 40.0%
!! GATTTTAGTAGCTCTTGTGG+AGG - chr5.2:59344461-59344480 None:intergenic 40.0%
!! GGAGATTTTAGTAGCTCTTG+TGG - chr5.2:59344464-59344483 None:intergenic 40.0%
!! TTTAGGTTGGTGAAGGAATG+GGG + chr5.2:59345379-59345398 MS.gene44888:intron 40.0%
!!! AGTTGAGGTTTTGGGACATA+AGG + chr5.2:59345246-59345265 MS.gene44888:intron 40.0%
!!! CTTCGATTTGGCGGTTTTTA+AGG - chr5.2:59344685-59344704 None:intergenic 40.0%
!!! CTTTTAGGTTGGTGAAGGAA+TGG + chr5.2:59345377-59345396 MS.gene44888:intron 40.0%
!!! GTTTTGGGACATAAGGCTAT+TGG + chr5.2:59345253-59345272 MS.gene44888:intron 40.0%
AAGGAATGGGGTGTAGGTTT+GGG + chr5.2:59345391-59345410 MS.gene44888:CDS 45.0%
AAGGGGTAGTGATGTTGTAG+CGG + chr5.2:59345432-59345451 MS.gene44888:CDS 45.0%
CAACAACTGATGATCACGGT+CGG - chr5.2:59344723-59344742 None:intergenic 45.0%
GAGAGGTTTAGAGACACCAA+AGG + chr5.2:59344839-59344858 MS.gene44888:CDS 45.0%
GTCTTCTGCATCAAGTTTCC+AGG + chr5.2:59344350-59344369 MS.gene44888:CDS 45.0%
TGTCCCAAAACCTCAACTTG+TGG - chr5.2:59345244-59345263 None:intergenic 45.0%
TTCATTCCTGCACCAGGAAT+TGG + chr5.2:59344260-59344279 MS.gene44888:CDS 45.0%
TTGGGACATAAGGCTATTGG+TGG + chr5.2:59345256-59345275 MS.gene44888:intron 45.0%
TTTAGTAGCTCTTGTGGAGG+TGG - chr5.2:59344458-59344477 None:intergenic 45.0%
! AGGAATGCTGTTGTTGATGG+TGG + chr5.2:59345541-59345560 MS.gene44888:CDS 45.0%
!! CTGAGAGTGGACTATAGAAG+GGG + chr5.2:59345415-59345434 MS.gene44888:CDS 45.0%
!! GCACCACAAGTTGAGGTTTT+GGG + chr5.2:59345238-59345257 MS.gene44888:intron 45.0%
!! GCTGAGAGTGGACTATAGAA+GGG + chr5.2:59345414-59345433 MS.gene44888:CDS 45.0%
AGGAATGGGGTGTAGGTTTG+GGG + chr5.2:59345392-59345411 MS.gene44888:CDS 50.0%
CTCATCTTCATTCCTGCACC+AGG + chr5.2:59344254-59344273 MS.gene44888:CDS 50.0%
GAAGCTAAGTGGCCAATTCC+TGG - chr5.2:59344275-59344294 None:intergenic 50.0%
GAAGGAATGGGGTGTAGGTT+TGG + chr5.2:59345390-59345409 MS.gene44888:CDS 50.0%
GGACCAACAGCATAGATAGG+AGG - chr5.2:59344938-59344957 None:intergenic 50.0%
TTGGTGAAGGAATGGGGTGT+AGG + chr5.2:59345385-59345404 MS.gene44888:CDS 50.0%
! GGAAGCATGGGAGTTAGCTT+TGG + chr5.2:59345070-59345089 MS.gene44888:CDS 50.0%
! TGTGGAGGTGGTTCTACTTC+AGG - chr5.2:59344446-59344465 None:intergenic 50.0%
!! GGCACCACAAGTTGAGGTTT+TGG + chr5.2:59345237-59345256 MS.gene44888:intron 50.0%
!! GGCTGAGAGTGGACTATAGA+AGG + chr5.2:59345413-59345432 MS.gene44888:CDS 50.0%
AAGTGGCCAATTCCTGGTGC+AGG - chr5.2:59344269-59344288 None:intergenic 55.0%
TAGTGATGTTGTAGCGGCCG+AGG + chr5.2:59345438-59345457 MS.gene44888:CDS 55.0%
TGGATGGGCACCACAAGTTG+AGG + chr5.2:59345231-59345250 MS.gene44888:intron 55.0%
! GAATCTGCAAAGGGTGTGCC+TGG - chr5.2:59344371-59344390 None:intergenic 55.0%
! TGTAGGTTTGGGGCTGAGAG+TGG + chr5.2:59345402-59345421 MS.gene44888:CDS 55.0%
GCGGCCGAGGAGATTGAGAA+AGG + chr5.2:59345451-59345470 MS.gene44888:CDS 60.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 59344221 59345618 59344221 ID=MS.gene44888
chr5.2 mRNA 59344221 59345618 59344221 ID=MS.gene44888.t1;Parent=MS.gene44888
chr5.2 exon 59344221 59345143 59344221 ID=MS.gene44888.t1.exon1;Parent=MS.gene44888.t1
chr5.2 CDS 59344221 59345143 59344221 ID=cds.MS.gene44888.t1;Parent=MS.gene44888.t1
chr5.2 exon 59345384 59345618 59345384 ID=MS.gene44888.t1.exon2;Parent=MS.gene44888.t1
chr5.2 CDS 59345384 59345618 59345384 ID=cds.MS.gene44888.t1;Parent=MS.gene44888.t1
Gene Sequence

>MS.gene44888

ATGTCTCTGATTGATGTAAACAAGAAATCAGAACTCATCTTCATTCCTGCACCAGGAATTGGCCACTTAGCTTCAGCTCTTGAATTTGCAAAACTTCTAACCAACCATGACAAAAATCTTTGCATCACAGTCTTCTGCATCAAGTTTCCAGGCACACCCTTTGCAGATTCATATATCAAATCAGTTTTATCCTCACAACCACAAATTCAACTCATTGATCTTCCTGAAGTAGAACCACCTCCACAAGAGCTACTAAAATCTCCAGAATTTTACATCTTGACTTTTTTGGAGAGTCTCATACCTCATGTCAAAGCAACCATCAAAACCATTTTATCAAACAAAATTGTTGGGTTAGTCCTAGATTTCTTTTGTGTTTCAATGATTGATGTTGGAAATGAATTTGGTATCCCTTCTTATTTGTTTTTAACATCAAATGTTGGTTTTTTAAGTCTCATGCTTTCCCTTAAAAACCGCCAAATCGAAGATGTTTTCGATGATACCGACCGTGATCATCAGTTGTTGAATATTCCTGGTATCTCAAATCAAGTTCCTTTTAATGTTTTACCTGATGCTTGTTTTAATAAAGATGGTGGATATGTTGCTTATTATAAACTAGCTGAGAGGTTTAGAGACACCAAAGGGATTATTGTTAATACCTTTTCAGATTTGGAACAATCTTCTATTGATGCATTATATGATTATGATGAAAAAATTCCTCCTATCTATGCTGTTGGTCCTTTGTTAGATCTCAAAGGTCAACCTAACCCTAAATTGGATCAAGCTCAGCATGATCTTATATTGAAATGGCTAGATGAGCAGCCAGATAAATCAGTTGTTTTTCTATGTTTTGGAAGCATGGGAGTTAGCTTTGGTCCCTCTCAAACAAGAGAAATAGCATTAGGACTTAAGCATAGTGGAGTTAGGTTCTTGTGGTCTAACAGTGCAGAGAAAAAAGTGTTTCCAGAAGGGTTTTTAGAATGGATGGAATTAGAAGGTAAGGGAATGATATGTGGATGGGCACCACAAGTTGAGGTTTTGGGACATAAGGCTATTGGTGGATTTGTTTCACATTGTGGATGGAATTCTATTTTGGAAAGTATGTGGTTTGGTGTACCAATATTGACATGGCCTATCTATGCAGAACAACAGCTTAATGCTTTTAGGTTGGTGAAGGAATGGGGTGTAGGTTTGGGGCTGAGAGTGGACTATAGAAGGGGTAGTGATGTTGTAGCGGCCGAGGAGATTGAGAAAGGATTGAAGGATTTGATGGATAAAGATAGCATTGTGCACAAGAAGGTTCAAGAGATCAAAGAGATGTCTAGGAATGCTGTTGTTGATGGTGGATCTTCTTTAATTTCTGTCGGAAAACTTATTGATGATATTACAGGAAGCAACTGA
Protein sequence

>MS.gene44888.t1

MSLIDVNKKSELIFIPAPGIGHLASALEFAKLLTNHDKNLCITVFCIKFPGTPFADSYIKSVLSSQPQIQLIDLPEVEPPPQELLKSPEFYILTFLESLIPHVKATIKTILSNKIVGLVLDFFCVSMIDVGNEFGIPSYLFLTSNVGFLSLMLSLKNRQIEDVFDDTDRDHQLLNIPGISNQVPFNVLPDACFNKDGGYVAYYKLAERFRDTKGIIVNTFSDLEQSSIDALYDYDEKIPPIYAVGPLLDLKGQPNPKLDQAQHDLILKWLDEQPDKSVVFLCFGSMGVSFGPSQTREIALGLKHSGVRLVKEWGVGLGLRVDYRRGSDVVAAEEIEKGLKDLMDKDSIVHKKVQEIKEMSRNAVVDGGSSLISVGKLIDDITGSN