AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene74758


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene74758.t1 MTR_7g009070 90.299 134 10 1 1 131 1 134 1.89e-79 230
MS.gene74758.t1 MTR_5g037950 55.385 130 54 1 1 130 1 126 9.14e-41 132
MS.gene74758.t1 MTR_5g037930 56.034 116 51 0 15 130 12 127 7.71e-39 127
MS.gene74758.t1 MTR_5g037890 54.701 117 53 0 14 130 9 125 8.19e-39 127
MS.gene74758.t1 MTR_1g023140 48.214 112 58 0 19 130 2 113 1.71e-38 126
MS.gene74758.t1 MTR_3g112410 46.847 111 59 0 19 129 2 112 1.19e-35 119
MS.gene74758.t1 MTR_5g021180 46.018 113 61 0 19 131 4 116 1.00e-34 117
MS.gene74758.t1 MTR_8g107460 42.574 101 58 0 29 129 260 360 1.55e-26 101
MS.gene74758.t1 MTR_7g073680 38.095 105 65 0 22 126 23 127 2.39e-25 93.6
MS.gene74758.t1 MTR_8g005320 35.238 105 68 0 22 126 25 129 2.01e-24 91.3
MS.gene74758.t1 MTR_2g010750 37.143 105 66 0 23 127 9 113 5.75e-24 89.7
MS.gene74758.t1 MTR_4g081380 36.697 109 69 0 18 126 5 113 9.89e-24 89.4
MS.gene74758.t1 MTR_8g006685 35.238 105 68 0 22 126 94 198 1.76e-23 90.9
MS.gene74758.t1 MTR_8g006560 35.238 105 68 0 22 126 94 198 1.76e-23 90.9
MS.gene74758.t1 MTR_2g082590 35.398 113 73 0 14 126 1 113 2.84e-23 87.8
MS.gene74758.t1 MTR_2g082590 39.806 103 60 1 24 126 26 126 6.31e-23 87.4
MS.gene74758.t1 MTR_1g098660 35.955 89 55 2 41 127 74 162 1.53e-18 77.0
MS.gene74758.t1 MTR_1g114290 38.298 94 53 3 35 127 77 166 1.25e-17 75.1
MS.gene74758.t1 MTR_3g089065 36.082 97 55 4 38 129 78 172 1.34e-17 74.7
MS.gene74758.t1 MTR_2g079420 36.145 83 51 2 48 128 89 171 1.57e-16 72.0
MS.gene74758.t1 MTR_7g102130 29.508 122 82 2 8 129 10 127 5.91e-16 69.3
MS.gene74758.t1 MTR_7g080250 35.484 124 69 5 2 122 57 172 1.55e-15 69.7
MS.gene74758.t1 MTR_8g059015 34.343 99 60 4 32 128 79 174 1.74e-15 69.3
MS.gene74758.t1 MTR_4g094690 31.707 82 54 1 49 128 85 166 4.98e-15 67.8
MS.gene74758.t1 MTR_4g085880 36.842 76 47 1 48 122 84 159 3.58e-14 65.9
MS.gene74758.t1 MTR_2g079340 32.955 88 57 2 44 129 51 138 4.10e-14 65.5
MS.gene74758.t1 MTR_4g088905 35.000 80 52 0 49 128 25 104 3.26e-12 62.4
MS.gene74758.t1 MTR_1g053160 32.468 77 51 1 43 118 425 501 2.87e-11 59.7
MS.gene74758.t1 MTR_3g112400 56.098 41 18 0 79 119 41 81 4.58e-11 55.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene74758.t1 AT5G39950 59.398 133 52 1 1 131 1 133 1.06e-56 173
MS.gene74758.t1 AT1G45145 52.475 101 48 0 29 129 11 111 8.86e-36 119
MS.gene74758.t1 AT3G51030 46.018 113 61 0 19 131 2 114 2.65e-34 115
MS.gene74758.t1 AT1G59730 42.748 131 71 2 1 130 1 128 6.50e-34 115
MS.gene74758.t1 AT1G19730 46.429 112 59 1 19 129 2 113 1.90e-33 113
MS.gene74758.t1 AT1G69880 51.546 97 47 0 33 129 47 143 2.96e-33 114
MS.gene74758.t1 AT5G42980 47.059 102 54 0 29 130 11 112 2.45e-31 108
MS.gene74758.t1 AT3G17880 40.000 105 63 0 25 129 265 369 9.69e-26 99.8
MS.gene74758.t1 AT3G17880 40.000 105 63 0 25 129 272 376 1.20e-25 99.8
MS.gene74758.t1 AT3G08710 39.062 128 74 2 1 126 1 126 2.11e-24 91.3
MS.gene74758.t1 AT3G08710 39.062 128 74 2 1 126 1 126 2.11e-24 91.3
MS.gene74758.t1 AT3G08710 39.062 128 74 2 1 126 1 126 2.11e-24 91.3
MS.gene74758.t1 AT3G56420 38.298 94 58 0 33 126 50 143 2.69e-24 91.7
MS.gene74758.t1 AT3G56420 38.298 94 58 0 33 126 50 143 2.69e-24 91.7
MS.gene74758.t1 AT3G56420 38.298 94 58 0 33 126 50 143 2.69e-24 91.7
MS.gene74758.t1 AT2G40790 38.298 94 58 0 33 126 50 143 1.30e-21 84.7
MS.gene74758.t1 AT2G40790 38.298 94 58 0 33 126 50 143 1.30e-21 84.7
MS.gene74758.t1 AT1G11530 38.144 97 60 0 30 126 9 105 2.41e-21 82.8
MS.gene74758.t1 AT1G43560 36.697 109 66 3 12 117 41 149 8.58e-20 80.1
MS.gene74758.t1 AT1G76760 39.744 78 46 1 41 117 77 154 6.32e-19 78.2
MS.gene74758.t1 AT1G50320 44.286 70 38 1 43 111 85 154 4.12e-17 73.6
MS.gene74758.t1 AT3G02730 36.782 87 54 1 46 131 88 174 5.82e-17 73.2
MS.gene74758.t1 AT3G15360 32.673 101 63 4 30 128 92 189 1.83e-16 72.4
MS.gene74758.t1 AT1G03680 36.145 83 51 2 48 128 95 177 5.47e-16 70.9
MS.gene74758.t1 AT4G03520 36.620 71 44 1 48 117 101 171 1.60e-15 69.7
MS.gene74758.t1 AT3G53220 26.549 113 81 1 17 129 15 125 1.60e-15 68.2
MS.gene74758.t1 AT1G31020 30.000 110 75 1 21 128 47 156 4.20e-15 67.8
MS.gene74758.t1 AT2G35010 34.146 82 52 1 49 128 110 191 4.82e-15 68.6
MS.gene74758.t1 AT2G35010 34.146 82 52 1 49 128 110 191 4.82e-15 68.6
MS.gene74758.t1 AT5G16400 32.184 87 58 1 46 131 98 184 1.66e-14 67.0
MS.gene74758.t1 AT4G04950 33.645 107 67 1 22 128 2 104 4.32e-14 67.8
MS.gene74758.t1 AT4G32580 31.868 91 62 0 38 128 14 104 4.53e-14 65.1
MS.gene74758.t1 AT1G52990 32.632 95 63 1 36 129 218 312 1.48e-13 65.9
MS.gene74758.t1 AT4G26160 32.292 96 61 2 38 129 105 200 2.25e-12 62.0
MS.gene74758.t1 AT2G41680 28.889 90 62 2 43 130 440 529 1.07e-11 61.2

Find 25 sgRNAs with CRISPR-Local

Find 66 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGATGTGGCACAAGAGTTTA+AGG 0.257673 7.1:+86083013 MS.gene74758:intron
AATCGATGTTGATGAATTAT+CGG 0.348841 7.1:+86082373 MS.gene74758:CDS
TTAGCCATAGCTTGAAGAAT+CGG 0.442276 7.1:-86082323 None:intergenic
CGTTTGTGTTGTTGAAGAAT+GGG 0.453169 7.1:+86083051 MS.gene74758:CDS
ACGTTTGTGTTGTTGAAGAA+TGG 0.456074 7.1:+86083050 MS.gene74758:CDS
TTCTCCTCCTTAGTCGGCAG+TGG 0.459284 7.1:+86082043 MS.gene74758:CDS
TACGGGGCCACTGCCGACTA+AGG 0.463959 7.1:-86082050 None:intergenic
TGATTGATTTCTCTGCTACA+TGG 0.471205 7.1:+86082276 MS.gene74758:CDS
TGATGATTCTGATGACTGTA+CGG 0.487264 7.1:-86082068 None:intergenic
GAATGGGAAAGAGGTTGATA+AGG 0.491854 7.1:+86083067 MS.gene74758:CDS
GATGATTCTGATGACTGTAC+GGG 0.500909 7.1:-86082067 None:intergenic
GATTTCTCTGCTACATGGTG+TGG 0.519111 7.1:+86082281 MS.gene74758:CDS
GGCACAAGAGTTTAAGGTGC+AGG 0.519916 7.1:+86083019 MS.gene74758:CDS
AAAGAGGTTGATAAGGTTGT+CGG 0.520686 7.1:+86083074 MS.gene74758:CDS
TAAGGTTGTCGGAGCAAAGA+AGG 0.540917 7.1:+86083085 MS.gene74758:CDS
GTGTGGACCTTGTAAAATGA+TGG 0.541235 7.1:+86082298 MS.gene74758:CDS
CCTTCCACTCATCTGCTCGC+TGG 0.549073 7.1:+86082110 MS.gene74758:CDS
GTTGTTGAAGAATGGGAAAG+AGG 0.553595 7.1:+86083058 MS.gene74758:CDS
AGATGAGTGGAAGGTCTTGA+CGG 0.570930 7.1:-86082101 None:intergenic
GGAACCGATTCTTCAAGCTA+TGG 0.574314 7.1:+86082319 MS.gene74758:CDS
GGGGCCACTGCCGACTAAGG+AGG 0.594415 7.1:-86082047 None:intergenic
GTTGCCAGCGAGCAGATGAG+TGG 0.605035 7.1:-86082114 None:intergenic
TTCTTCAACAACACAAACGT+TGG 0.610426 7.1:-86083047 None:intergenic
CCAGCGAGCAGATGAGTGGA+AGG 0.627549 7.1:-86082110 None:intergenic
ATGATTCTGATGACTGTACG+GGG 0.715538 7.1:-86082066 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTATGTTATTTGTTAAAA+TGG + chr7.1:86082547-86082566 MS.gene74758:intron 10.0%
!!! TCATTTTTACAAATTATTAT+CGG + chr7.1:86082904-86082923 MS.gene74758:intron 10.0%
!! CCAAAAAATAGAAAAATAAA+TGG - chr7.1:86082212-86082231 None:intergenic 15.0%
!!! AATTATACAAAAAGCATTTA+AGG + chr7.1:86082678-86082697 MS.gene74758:intron 15.0%
!!! ATAATGATTTAATTTTTGTG+TGG + chr7.1:86082971-86082990 MS.gene74758:intron 15.0%
!!! ATTGTATGTTATTTTTTTGA+GGG + chr7.1:86082520-86082539 MS.gene74758:intron 15.0%
!!! CCATTTATTTTTCTATTTTT+TGG + chr7.1:86082209-86082228 MS.gene74758:intron 15.0%
!!! TGATAATGGTAAATTTTAAA+AGG - chr7.1:86082723-86082742 None:intergenic 15.0%
!!! AACTAAAAATACATTTTTGC+CGG + chr7.1:86082614-86082633 MS.gene74758:intron 20.0%
!!! ATGTTATTTGTTAAAATGGA+TGG + chr7.1:86082551-86082570 MS.gene74758:intron 20.0%
!!! ATTTTAAAAGGAAAAAGGTT+AGG - chr7.1:86082711-86082730 None:intergenic 20.0%
!!! CATTGTATGTTATTTTTTTG+AGG + chr7.1:86082519-86082538 MS.gene74758:intron 20.0%
!!! GGTAAATTTTAAAAGGAAAA+AGG - chr7.1:86082716-86082735 None:intergenic 20.0%
!!! TTAATTTTTGTGTGGTTTTT+TGG + chr7.1:86082979-86082998 MS.gene74758:intron 20.0%
! AACAGTATAGTTGATCAAAT+TGG - chr7.1:86082413-86082432 None:intergenic 25.0%
! ACAGTATAGTTGATCAAATT+GGG - chr7.1:86082412-86082431 None:intergenic 25.0%
! ATTGAGATCTAAAGAGATAT+GGG - chr7.1:86082181-86082200 None:intergenic 25.0%
! CAAATAATAACACATCGAAT+CGG - chr7.1:86082803-86082822 None:intergenic 25.0%
! TATTGAGATCTAAAGAGATA+TGG - chr7.1:86082182-86082201 None:intergenic 25.0%
! TTCTCAAAGTTTATTATAGC+CGG - chr7.1:86082636-86082655 None:intergenic 25.0%
!! AATCGATGTTGATGAATTAT+CGG + chr7.1:86082373-86082392 MS.gene74758:CDS 25.0%
!! ACCATATTTTACTTACTTGA+CGG - chr7.1:86082764-86082783 None:intergenic 25.0%
!!! TTTTGATTTTCTGTTTGTGA+AGG + chr7.1:86082250-86082269 MS.gene74758:intron 25.0%
ACCGTCAAGTAAGTAAAATA+TGG + chr7.1:86082760-86082779 MS.gene74758:intron 30.0%
AGAAGCAATAACAACACATA+TGG - chr7.1:86082941-86082960 None:intergenic 30.0%
GAAGGATGAACTTAAAAACA+AGG + chr7.1:86083103-86083122 MS.gene74758:CDS 30.0%
TTAAAAGGAAAAAGGTTAGG+TGG - chr7.1:86082708-86082727 None:intergenic 30.0%
! TTCTTTAGATGTTTTTCGTG+TGG + chr7.1:86082586-86082605 MS.gene74758:intron 30.0%
!! ATTTTTGTGTGGTTTTTTGG+TGG + chr7.1:86082982-86083001 MS.gene74758:intron 30.0%
!! TTTTTGTGTGGTTTTTTGGT+GGG + chr7.1:86082983-86083002 MS.gene74758:intron 30.0%
TGATGATTCTGATGACTGTA+CGG - chr7.1:86082071-86082090 None:intergenic 35.0%
TTAGCCATAGCTTGAAGAAT+CGG - chr7.1:86082326-86082345 None:intergenic 35.0%
TTCTTCAACAACACAAACGT+TGG - chr7.1:86083050-86083069 None:intergenic 35.0%
! ACGTTTGTGTTGTTGAAGAA+TGG + chr7.1:86083050-86083069 MS.gene74758:CDS 35.0%
! CGTTTGTGTTGTTGAAGAAT+GGG + chr7.1:86083051-86083070 MS.gene74758:CDS 35.0%
! TGATTGATTTCTCTGCTACA+TGG + chr7.1:86082276-86082295 MS.gene74758:CDS 35.0%
! TGATTTTCTGTTTGTGAAGG+TGG + chr7.1:86082253-86082272 MS.gene74758:intron 35.0%
!! AAAGAGGTTGATAAGGTTGT+CGG + chr7.1:86083074-86083093 MS.gene74758:CDS 35.0%
!! ATCGGTTCCATCATTTTACA+AGG - chr7.1:86082308-86082327 None:intergenic 35.0%
!! TCATTTTTCTCCTCCTTAGT+CGG + chr7.1:86082037-86082056 MS.gene74758:CDS 35.0%
!!! AATTTTTAGCACCGACACTT+CGG + chr7.1:86082447-86082466 MS.gene74758:intron 35.0%
!!! GGTTTTTTGGTGGGAATTAT+AGG + chr7.1:86082992-86083011 MS.gene74758:intron 35.0%
ACACGGACAAGACATGATAA+TGG - chr7.1:86082737-86082756 None:intergenic 40.0%
ACTTACTTGACGGTCTAACA+CGG - chr7.1:86082754-86082773 None:intergenic 40.0%
ATGATTCTGATGACTGTACG+GGG - chr7.1:86082069-86082088 None:intergenic 40.0%
GATGATTCTGATGACTGTAC+GGG - chr7.1:86082070-86082089 None:intergenic 40.0%
GTGTGGACCTTGTAAAATGA+TGG + chr7.1:86082298-86082317 MS.gene74758:CDS 40.0%
GTTGTTGAAGAATGGGAAAG+AGG + chr7.1:86083058-86083077 MS.gene74758:CDS 40.0%
TTGGTGGGAATTATAGGATG+TGG + chr7.1:86082998-86083017 MS.gene74758:intron 40.0%
!! GAATGGGAAAGAGGTTGATA+AGG + chr7.1:86083067-86083086 MS.gene74758:CDS 40.0%
AAACCCTCTATCCGAAGTGT+CGG - chr7.1:86082461-86082480 None:intergenic 45.0%
AGATGAGTGGAAGGTCTTGA+CGG - chr7.1:86082104-86082123 None:intergenic 45.0%
GATTTCTCTGCTACATGGTG+TGG + chr7.1:86082281-86082300 MS.gene74758:CDS 45.0%
GGATGTGGCACAAGAGTTTA+AGG + chr7.1:86083013-86083032 MS.gene74758:intron 45.0%
TAAGGTTGTCGGAGCAAAGA+AGG + chr7.1:86083085-86083104 MS.gene74758:CDS 45.0%
! GATATGGGTTTTACGAGACG+AGG - chr7.1:86082166-86082185 None:intergenic 45.0%
! GGAACCGATTCTTCAAGCTA+TGG + chr7.1:86082319-86082338 MS.gene74758:CDS 45.0%
! GGCACAAGAGTTTAAGGTGC+AGG + chr7.1:86083019-86083038 MS.gene74758:CDS 50.0%
TTCTCCTCCTTAGTCGGCAG+TGG + chr7.1:86082043-86082062 MS.gene74758:CDS 55.0%
! GCACCGACACTTCGGATAGA+GGG + chr7.1:86082455-86082474 MS.gene74758:intron 55.0%
!! AGCACCGACACTTCGGATAG+AGG + chr7.1:86082454-86082473 MS.gene74758:intron 55.0%
CCAGCGAGCAGATGAGTGGA+AGG - chr7.1:86082113-86082132 None:intergenic 60.0%
CCTTCCACTCATCTGCTCGC+TGG + chr7.1:86082110-86082129 MS.gene74758:CDS 60.0%
GTTGCCAGCGAGCAGATGAG+TGG - chr7.1:86082117-86082136 None:intergenic 60.0%
! TACGGGGCCACTGCCGACTA+AGG - chr7.1:86082053-86082072 None:intergenic 65.0%
! GGGGCCACTGCCGACTAAGG+AGG - chr7.1:86082050-86082069 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr7.1 gene 86082031 86083145 86082031 ID=MS.gene74758
chr7.1 mRNA 86082031 86083145 86082031 ID=MS.gene74758.t1;Parent=MS.gene74758
chr7.1 exon 86082031 86082171 86082031 ID=MS.gene74758.t1.exon1;Parent=MS.gene74758.t1
chr7.1 CDS 86082031 86082171 86082031 ID=cds.MS.gene74758.t1;Parent=MS.gene74758.t1
chr7.1 exon 86082272 86082394 86082272 ID=MS.gene74758.t1.exon2;Parent=MS.gene74758.t1
chr7.1 CDS 86082272 86082394 86082272 ID=cds.MS.gene74758.t1;Parent=MS.gene74758.t1
chr7.1 exon 86083014 86083145 86083014 ID=MS.gene74758.t1.exon3;Parent=MS.gene74758.t1
chr7.1 CDS 86083014 86083145 86083014 ID=cds.MS.gene74758.t1;Parent=MS.gene74758.t1
Gene Sequence

>MS.gene74758

ATGGGATCATTTTTCTCCTCCTTAGTCGGCAGTGGCCCCGTACAGTCATCAGAATCATCAGAGAGTTCTTCCGTCAAGACCTTCCACTCATCTGCTCGCTGGCAACTTCACTTCAACGAACTCAAAGATTCTCCTCGTCTCGTAAAACCCATATCTCTTTAGATCTCAATAATTTTAACCATTTATTTTTCTATTTTTTGGATCTGAAATTCTGAATGCTTTTGATTTTCTGTTTGTGAAGGTGGTGATTGATTTCTCTGCTACATGGTGTGGACCTTGTAAAATGATGGAACCGATTCTTCAAGCTATGGCTAATGAATTCACTGATGTTGAATTCATCAAAATCGATGTTGATGAATTATCGGTAATATTCTTATACCCAATTTGATCAACTATACTGTTTTTAATTTCAGTAAAATTTTTAGCACCGACACTTCGGATAGAGGGTTTATCACACTTGTCAGCATGCATCGAACACAACACTGACACATTGTATGTTATTTTTTTGAGGGATTCAATTATGTTATTTGTTAAAATGGATGGAAACTAACACATTTCTTTAGATGTTTTTCGTGTGGTTCTTAACTAAAAATACATTTTTGCCGGCTATAATAAACTTTGAGAAAATCATGAGTAGTTTAATTCAGAATTATACAAAAAGCATTTAAGGAAATCCACCTAACCTTTTTCCTTTTAAAATTTACCATTATCATGTCTTGTCCGTGTTAGACCGTCAAGTAAGTAAAATATGGTCAAAAATTGATTCACTCCGATTCGATGTGTTATTATTTGCATCAATGCTTTGTAGCATATTTATTACGTGTTTGTATTGTATCATAGTACAATACAAACACATGATTACATTCAATTATGTCATTTTTACAAATTATTATCGGTGTAGATGTGTCCATATGTGTTGTTATTGCTTCTTAGCATATTTATAATGATTTAATTTTTGTGTGGTTTTTTGGTGGGAATTATAGGATGTGGCACAAGAGTTTAAGGTGCAGGCTATGCCAACGTTTGTGTTGTTGAAGAATGGGAAAGAGGTTGATAAGGTTGTCGGAGCAAAGAAGGATGAACTTAAAAACAAGGTTCAAAAGCACAAAGCTTAG

Protein sequence

>MS.gene74758.t1

MGSFFSSLVGSGPVQSSESSESSSVKTFHSSARWQLHFNELKDSPRLVVIDFSATWCGPCKMMEPILQAMANEFTDVEFIKIDVDELSDVAQEFKVQAMPTFVLLKNGKEVDKVVGAKKDELKNKVQKHKA