AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene052866


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene052866.t1 MTR_8g465890 96.324 408 15 0 1 408 85 492 0.0 812
MS.gene052866.t1 MTR_2g047855 86.098 410 51 3 1 408 89 494 0.0 712
MS.gene052866.t1 MTR_2g090290 51.459 377 173 2 12 381 101 474 2.75e-130 384
MS.gene052866.t1 MTR_8g059115 30.769 403 268 7 1 398 1 397 8.16e-60 200
MS.gene052866.t1 MTR_8g069570 32.958 355 229 5 4 353 83 433 4.19e-58 197
MS.gene052866.t1 MTR_8g007375 31.285 358 239 5 7 361 8 361 4.58e-52 179
MS.gene052866.t1 MTR_2g013450 34.277 318 203 4 15 330 100 413 8.65e-51 178
MS.gene052866.t1 MTR_1g072300 31.148 366 241 6 1 361 95 454 4.03e-48 171
MS.gene052866.t1 MTR_3g099753 29.570 372 239 8 1 360 95 455 2.01e-45 164
MS.gene052866.t1 MTR_3g099753 29.570 372 239 8 1 360 119 479 2.27e-45 164
MS.gene052866.t1 MTR_1g086820 30.747 348 228 6 5 347 92 431 1.60e-44 161
MS.gene052866.t1 MTR_1g022400 29.643 280 189 6 1 276 112 387 1.04e-26 110
MS.gene052866.t1 MTR_7g089770 25.201 373 251 12 1 364 214 567 1.39e-23 103
MS.gene052866.t1 MTR_1g050750 23.784 370 260 9 1 364 148 501 1.31e-17 85.1
MS.gene052866.t1 MTR_1g048080 24.841 314 218 8 46 353 340 641 8.62e-15 77.0
MS.gene052866.t1 MTR_8g031510 28.906 128 90 1 235 361 1 128 1.15e-12 66.2
MS.gene052866.t1 MTR_8g098755 22.713 317 229 8 47 354 240 549 3.34e-12 68.6
MS.gene052866.t1 MTR_8g031520 30.303 132 84 3 11 139 173 299 4.28e-12 67.0
MS.gene052866.t1 MTR_7g091410 23.263 331 211 9 25 316 130 456 9.38e-12 67.0
MS.gene052866.t1 MTR_5g077220 22.222 270 201 8 46 312 466 729 1.04e-11 67.4
MS.gene052866.t1 MTR_7g091410 23.263 331 211 9 25 316 171 497 1.07e-11 67.0
MS.gene052866.t1 MTR_7g091410 22.590 332 214 8 24 316 235 562 1.18e-11 67.0
MS.gene052866.t1 MTR_7g091410 23.193 332 212 9 24 316 374 701 1.21e-11 67.0
MS.gene052866.t1 MTR_2g096880 20.882 340 259 5 13 347 113 447 1.51e-11 66.2
MS.gene052866.t1 MTR_7g066990 20.552 326 245 8 18 338 194 510 3.74e-11 65.5
MS.gene052866.t1 MTR_3g033040 24.280 243 176 5 36 275 195 432 8.37e-11 63.9
MS.gene052866.t1 MTR_3g033040 24.280 243 176 5 36 275 195 432 9.06e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene052866.t1 AT2G20710 39.835 364 215 2 1 363 1 361 1.07e-97 297
MS.gene052866.t1 AT2G20710 39.835 364 215 2 1 363 96 456 2.87e-96 296
MS.gene052866.t1 AT1G60770 34.637 358 222 7 4 353 83 436 5.33e-63 210
MS.gene052866.t1 AT1G02150 31.877 389 250 8 7 384 132 516 7.73e-58 197
MS.gene052866.t1 AT5G27460 32.162 370 234 7 1 360 96 458 1.30e-52 183
MS.gene052866.t1 AT4G21705 32.704 318 206 5 15 328 97 410 1.10e-49 175
MS.gene052866.t1 AT4G01990 31.507 365 242 5 5 366 101 460 8.02e-47 167
MS.gene052866.t1 AT1G28020 34.323 303 181 5 1 294 94 387 3.15e-43 158
MS.gene052866.t1 AT4G02820 27.151 372 264 5 1 371 119 484 1.14e-40 151
MS.gene052866.t1 AT1G02370 31.119 286 190 5 4 286 131 412 3.55e-38 145
MS.gene052866.t1 AT5G09450 30.682 264 178 4 12 272 124 385 7.22e-25 105
MS.gene052866.t1 AT3G11380 28.571 287 163 7 4 284 103 353 1.31e-24 106
MS.gene052866.t1 AT3G11380 34.752 141 86 4 1 138 404 541 6.22e-12 67.8
MS.gene052866.t1 AT1G80270 26.359 368 246 9 5 364 190 540 3.93e-23 102
MS.gene052866.t1 AT1G80270 26.359 368 246 9 5 364 190 540 3.93e-23 102
MS.gene052866.t1 AT1G80270 26.359 368 246 9 5 364 190 540 3.93e-23 102
MS.gene052866.t1 AT1G80270 26.359 368 246 9 5 364 190 540 3.93e-23 102
MS.gene052866.t1 AT1G80270 26.359 368 246 9 5 364 190 540 3.93e-23 102
MS.gene052866.t1 AT2G20720 28.826 281 167 11 88 353 1 263 1.12e-22 97.8
MS.gene052866.t1 AT5G04810 25.962 312 219 7 45 353 345 647 1.67e-18 88.6
MS.gene052866.t1 AT5G04810 25.962 312 219 7 45 353 345 647 1.75e-18 88.2
MS.gene052866.t1 AT1G28000 28.761 226 146 6 9 225 99 318 5.92e-18 85.5
MS.gene052866.t1 AT1G15480 21.972 355 262 6 13 364 196 538 3.08e-15 78.2
MS.gene052866.t1 AT2G01740 20.536 336 259 5 18 350 63 393 5.86e-14 73.9
MS.gene052866.t1 AT1G62590 22.222 333 233 8 43 365 259 575 7.12e-12 67.8
MS.gene052866.t1 AT1G62590 22.222 333 233 8 43 365 259 575 7.12e-12 67.8
MS.gene052866.t1 AT1G63330 22.253 364 246 10 12 365 164 500 2.33e-11 65.9
MS.gene052866.t1 AT1G63330 22.253 364 246 10 12 365 164 500 2.33e-11 65.9
MS.gene052866.t1 AT1G63330 22.253 364 246 10 12 365 164 500 2.33e-11 65.9
MS.gene052866.t1 AT5G61990 21.994 341 226 7 70 374 440 776 3.01e-11 65.9
MS.gene052866.t1 AT1G12300 22.507 351 239 10 47 388 276 602 5.06e-11 65.1
MS.gene052866.t1 AT3G15590 23.018 391 282 9 6 390 204 581 9.24e-11 64.3
MS.gene052866.t1 AT3G15590 23.018 391 282 9 6 390 204 581 9.24e-11 64.3

Find 0 sgRNAs with CRISPR-Local

Find 81 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CTATTATAAGTATAACGATA+TGG - 31140:19102-19121 MS.gene052866:CDS 20.0%
!! TAAATTAGATGGAAGAATAT+GGG - 31140:19063-19082 MS.gene052866:CDS 20.0%
! AAAAGAGATGGTAAATTAGA+TGG - 31140:19052-19071 MS.gene052866:CDS 25.0%
! AATATCTGCAAAAGCTTAAA+TGG - 31140:18833-18852 MS.gene052866:CDS 25.0%
! AATGCATTGAAACTATTGAA+AGG - 31140:19343-19362 MS.gene052866:CDS 25.0%
! AGAAACCTTCATTTATTATC+AGG - 31140:18212-18231 MS.gene052866:CDS 25.0%
! ATAATTTATCAACTCATCAC+AGG + 31140:19299-19318 None:intergenic 25.0%
! GTAAATTAGATGGAAGAATA+TGG - 31140:19062-19081 MS.gene052866:CDS 25.0%
! TACAATGAAGAAAGCAATAT+TGG - 31140:19138-19157 MS.gene052866:CDS 25.0%
! TATCTCCTGATAATAAATGA+AGG + 31140:18220-18239 None:intergenic 25.0%
! TGACATTAACATAGAAATGT+AGG + 31140:18873-18892 None:intergenic 25.0%
! TTATTGTAAGAATGGTATGT+TGG - 31140:18997-19016 MS.gene052866:CDS 25.0%
AAATCTGAACAGTTGATTAG+AGG - 31140:18728-18747 MS.gene052866:CDS 30.0%
ACACACTTCAAAAGATTTCA+AGG - 31140:18313-18332 MS.gene052866:CDS 30.0%
AGGATGATGTGTATAGAATT+TGG - 31140:18810-18829 MS.gene052866:CDS 30.0%
ATACATGCAGCAAAAGTAAA+TGG + 31140:19190-19209 None:intergenic 30.0%
ATCACAACATAACTTTGAAC+TGG - 31140:18633-18652 MS.gene052866:CDS 30.0%
CATGAAAGAAAAGAGAGATT+TGG - 31140:19216-19235 MS.gene052866:CDS 30.0%
GTTAGTGCTTATTGTAAGAA+TGG - 31140:18989-19008 MS.gene052866:CDS 30.0%
TAACTAAGTATGCTGCTATT+GGG - 31140:18783-18802 MS.gene052866:CDS 30.0%
TAAGAATGGTATGTTGGAAA+AGG - 31140:19003-19022 MS.gene052866:CDS 30.0%
TACATGCAGCAAAAGTAAAT+GGG + 31140:19189-19208 None:intergenic 30.0%
TACTAGTAACATAGATGAGA+TGG - 31140:18583-18602 MS.gene052866:CDS 30.0%
TCAAAAGATTTCAAGGTCTA+TGG - 31140:18320-18339 MS.gene052866:CDS 30.0%
TCGAATCTAAATGACATTGA+TGG - 31140:18896-18915 MS.gene052866:CDS 30.0%
TTCCAATAATGAACAAGACT+TGG - 31140:18421-18440 MS.gene052866:CDS 30.0%
TTCTGTTAAAGCTCTATTCT+CGG - 31140:18459-18478 MS.gene052866:CDS 30.0%
! ACATAGAAATGTAGGTTGAA+TGG + 31140:18865-18884 None:intergenic 30.0%
! GCTGATGGATATATTAAAGT+TGG - 31140:18674-18693 MS.gene052866:CDS 30.0%
!! GAGTTGATAAATTATGTGCA+AGG - 31140:19304-19323 MS.gene052866:CDS 30.0%
!! TACCAAGTCTTGTTCATTAT+TGG + 31140:18426-18445 None:intergenic 30.0%
!!! TTGAAATCTTTTGAAGTGTG+TGG + 31140:18314-18333 None:intergenic 30.0%
ACAATACAACTCTGTTGAGA+GGG - 31140:18364-18383 MS.gene052866:CDS 35.0%
AGATGAGATGGAGAAGTTAT+TGG - 31140:18595-18614 MS.gene052866:CDS 35.0%
CTAACTAAGTATGCTGCTAT+TGG - 31140:18782-18801 MS.gene052866:CDS 35.0%
TAAAGTTGGCCAATTTGACA+AGG - 31140:18688-18707 MS.gene052866:CDS 35.0%
TAAGCACTAACCATCACATT+TGG + 31140:18980-18999 None:intergenic 35.0%
TGAAAGGAGATTATCATCTG+AGG - 31140:19359-19378 MS.gene052866:CDS 35.0%
TGTCAGAATTCCAAATGTGA+TGG - 31140:18967-18986 MS.gene052866:CDS 35.0%
! AAAAGGCAGAAGCATATGTT+GGG - 31140:19020-19039 MS.gene052866:CDS 35.0%
! AGAAAAGAGAGATTTGGAGT+TGG - 31140:19222-19241 MS.gene052866:CDS 35.0%
! AGAAATGTAGGTTGAATGGT+GGG + 31140:18861-18880 None:intergenic 35.0%
! TAGAAATGTAGGTTGAATGG+TGG + 31140:18862-18881 None:intergenic 35.0%
ACAGTTCCACACTTACTAGT+TGG - 31140:18543-18562 MS.gene052866:CDS 40.0%
ATTGAGCCAACTAGTAAGTG+TGG + 31140:18552-18571 None:intergenic 40.0%
CACAATACAACTCTGTTGAG+AGG - 31140:18363-18382 MS.gene052866:CDS 40.0%
CTATCAACGCCTTGTCAAAT+TGG + 31140:18700-18719 None:intergenic 40.0%
GCAGCAAAAGTAAATGGGTA+AGG + 31140:19184-19203 None:intergenic 40.0%
GTATGCTGCTATTGGGAAAA+AGG - 31140:18790-18809 MS.gene052866:CDS 40.0%
TGGAAGAATATGGGATCGTT+TGG - 31140:19072-19091 MS.gene052866:CDS 40.0%
TTAAAGCTCTATTCTCGGAC+AGG - 31140:18464-18483 MS.gene052866:CDS 40.0%
! AGTTGGCATTGGAGATTCTT+GGG - 31140:19239-19258 MS.gene052866:CDS 40.0%
! ATTCTAGAGGAATGGGAATC+TGG - 31140:18929-18948 MS.gene052866:CDS 40.0%
! GAAAAGGCAGAAGCATATGT+TGG - 31140:19019-19038 MS.gene052866:CDS 40.0%
! TGAGACGATTCTAGAGGAAT+GGG - 31140:18922-18941 MS.gene052866:CDS 40.0%
!! GGGAGGCTTTTAAAAAGAGA+TGG - 31140:19040-19059 MS.gene052866:CDS 40.0%
ACCTTGATAGGTAAAGTCCG+CGG - 31140:18254-18273 MS.gene052866:CDS 45.0%
ACGTATTCTGCTGTTGCTGA+TGG - 31140:18659-18678 MS.gene052866:CDS 45.0%
GAGCAACCAACTTCTCATGT+TGG + 31140:18490-18509 None:intergenic 45.0%
GCAATATTGGCAAGTCCACA+AGG - 31140:19151-19170 MS.gene052866:CDS 45.0%
GGAGAAGTTATTGGCGAAGA+TGG - 31140:18604-18623 MS.gene052866:CDS 45.0%
TATTGGCAAGTCCACAAGGA+TGG - 31140:19155-19174 MS.gene052866:CDS 45.0%
TCTGAGGATTGATGAAGTGC+CGG - 31140:19375-19394 MS.gene052866:CDS 45.0%
! CTGAGACGATTCTAGAGGAA+TGG - 31140:18921-18940 MS.gene052866:CDS 45.0%
! GAGAGATTTGGAGTTGGCAT+TGG - 31140:19228-19247 MS.gene052866:CDS 45.0%
! GAGTTGGCATTGGAGATTCT+TGG - 31140:19238-19257 MS.gene052866:CDS 45.0%
ACCGCGGACTTTACCTATCA+AGG + 31140:18258-18277 None:intergenic 50.0%
ATATGGGATCGTTTGGCGCA+TGG - 31140:19079-19098 MS.gene052866:CDS 50.0%
GACAGGCCAACATGAGAAGT+TGG - 31140:18481-18500 MS.gene052866:CDS 50.0%
GCGATACGTCTCACCTTGAT+AGG - 31140:18242-18261 MS.gene052866:CDS 50.0%
GGAGATTCTTGGGACGTGTA+GGG - 31140:19249-19268 MS.gene052866:CDS 50.0%
GGGTAAGGTTTCCATCCTTG+TGG + 31140:19169-19188 None:intergenic 50.0%
TCTCTGCTTGATCTAGACCG+CGG + 31140:18274-18293 None:intergenic 50.0%
TGGAGATTCTTGGGACGTGT+AGG - 31140:19248-19267 MS.gene052866:CDS 50.0%
TGGAGCTGAGACGATTCTAG+AGG - 31140:18916-18935 MS.gene052866:CDS 50.0%
! AGGCAGAAGCATATGTTGGG+AGG - 31140:19023-19042 MS.gene052866:CDS 50.0%
!! ATTGATGAAGTGCCGGTGGA+TGG - 31140:19382-19401 MS.gene052866:CDS 50.0%
CTTGGGACGTGTAGGGAACT+AGG - 31140:19256-19275 MS.gene052866:CDS 55.0%
TGTTGCTCCTCTCCATCCAC+CGG + 31140:19397-19416 None:intergenic 55.0%
!! GAGGATTGATGAAGTGCCGG+TGG - 31140:19378-19397 MS.gene052866:CDS 55.0%
!! TGAAGTGCCGGTGGATGGAG+AGG - 31140:19387-19406 MS.gene052866:CDS 60.0%


Chromosome Type Strat End Strand Name
31140 gene 18200 19426 18200 ID=MS.gene052866
31140 mRNA 18200 19426 18200 ID=MS.gene052866.t1;Parent=MS.gene052866
31140 exon 18200 19426 18200 ID=MS.gene052866.t1.exon1;Parent=MS.gene052866.t1
31140 CDS 18200 19426 18200 ID=cds.MS.gene052866.t1;Parent=MS.gene052866.t1
Gene Sequence

>MS.gene052866

ATGAGCAATGAAAGAAACCTTCATTTATTATCAGGAGATATTGCGATACGTCTCACCTTGATAGGTAAAGTCCGCGGTCTAGATCAAGCAGAGAAATATTTTAACAGTGTTCCACACACTTCAAAAGATTTCAAGGTCTATGGTGCTCTGTTGAATTGCTATGCACAATACAACTCTGTTGAGAGGGCAGAACTTATCATGAAGACATTAAAAGAGTCTTCTTCCAATAATGAACAAGACTTGGTAGTGAGTTATCATGTTCTGTTAAAGCTCTATTCTCGGACAGGCCAACATGAGAAGTTGGTTGCTCTGATGAAAGAAATGAAAGAGAAGAAAATGTGCAACAGTTCCACACTTACTAGTTGGCTCAATGCTTATGCGATTACTAGTAACATAGATGAGATGGAGAAGTTATTGGCGAAGATGGAAGTTGATCACAACATAACTTTGAACTGGCTCACGTATTCTGCTGTTGCTGATGGATATATTAAAGTTGGCCAATTTGACAAGGCGTTGATAGTGTTGAAAAAATCTGAACAGTTGATTAGAGGAAAGTCGAAGAGAGCTGTGTATCAATCTCTTCTAACTAAGTATGCTGCTATTGGGAAAAAGGATGATGTGTATAGAATTTGGAATATCTGCAAAAGCTTAAATGGTTCCCACCATTCAACCTACATTTCTATGTTAATGTCATTATCGAATCTAAATGACATTGATGGAGCTGAGACGATTCTAGAGGAATGGGAATCTGGAAATACATGCTTTGATGTCAGAATTCCAAATGTGATGGTTAGTGCTTATTGTAAGAATGGTATGTTGGAAAAGGCAGAAGCATATGTTGGGAGGCTTTTAAAAAGAGATGGTAAATTAGATGGAAGAATATGGGATCGTTTGGCGCATGGCTATTATAAGTATAACGATATGGATAAAGCAGTTCATACAATGAAGAAAGCAATATTGGCAAGTCCACAAGGATGGAAACCTTACCCATTTACTTTTGCTGCATGTATTGATCACATGAAAGAAAAGAGAGATTTGGAGTTGGCATTGGAGATTCTTGGGACGTGTAGGGAACTAGGTCATTTCTCTCAAGCTACCTGTGATGAGTTGATAAATTATGTGCAAGGTGAAATCTCAGAAACAAATGCATTGAAACTATTGAAAGGAGATTATCATCTGAGGATTGATGAAGTGCCGGTGGATGGAGAGGAGCAACATGAAATGTAG

Protein sequence

>MS.gene052866.t1

MSNERNLHLLSGDIAIRLTLIGKVRGLDQAEKYFNSVPHTSKDFKVYGALLNCYAQYNSVERAELIMKTLKESSSNNEQDLVVSYHVLLKLYSRTGQHEKLVALMKEMKEKKMCNSSTLTSWLNAYAITSNIDEMEKLLAKMEVDHNITLNWLTYSAVADGYIKVGQFDKALIVLKKSEQLIRGKSKRAVYQSLLTKYAAIGKKDDVYRIWNICKSLNGSHHSTYISMLMSLSNLNDIDGAETILEEWESGNTCFDVRIPNVMVSAYCKNGMLEKAEAYVGRLLKRDGKLDGRIWDRLAHGYYKYNDMDKAVHTMKKAILASPQGWKPYPFTFAACIDHMKEKRDLELALEILGTCRELGHFSQATCDELINYVQGEISETNALKLLKGDYHLRIDEVPVDGEEQHEM