Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73596.t1 | XP_024625600.1 | 98 | 101 | 2 | 0 | 1 | 101 | 1 | 101 | 1.10E-49 | 206.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73596.t1 | Q9LSD8 | 72.1 | 104 | 29 | 0 | 1 | 104 | 1 | 104 | 3.8e-40 | 165.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73596.t1 | A0A072TWY4 | 94.3 | 105 | 6 | 0 | 1 | 105 | 1 | 105 | 1.0e-49 | 205.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049230 | MS.gene73596 | 0.807332 | 5.32E-50 | -1.69E-46 |
| MS.gene049254 | MS.gene73596 | 0.808183 | 3.51E-50 | -1.69E-46 |
| MS.gene049272 | MS.gene73596 | 0.802032 | 6.80E-49 | -1.69E-46 |
| MS.gene049378 | MS.gene73596 | 0.808122 | 3.62E-50 | -1.69E-46 |
| MS.gene049404 | MS.gene73596 | 0.803351 | 3.63E-49 | -1.69E-46 |
| MS.gene049669 | MS.gene73596 | 0.825639 | 4.20E-54 | -1.69E-46 |
| MS.gene050105 | MS.gene73596 | 0.804498 | 2.10E-49 | -1.69E-46 |
| MS.gene050226 | MS.gene73596 | 0.805912 | 1.06E-49 | -1.69E-46 |
| MS.gene050227 | MS.gene73596 | 0.814066 | 1.86E-51 | -1.69E-46 |
| MS.gene050228 | MS.gene73596 | 0.818912 | 1.53E-52 | -1.69E-46 |
| MS.gene050355 | MS.gene73596 | 0.813805 | 2.12E-51 | -1.69E-46 |
| MS.gene050442 | MS.gene73596 | 0.809236 | 2.09E-50 | -1.69E-46 |
| MS.gene050559 | MS.gene73596 | 0.800674 | 1.29E-48 | -1.69E-46 |
| MS.gene050605 | MS.gene73596 | 0.80835 | 3.23E-50 | -1.69E-46 |
| MS.gene050680 | MS.gene73596 | 0.818798 | 1.62E-52 | -1.69E-46 |
| MS.gene050719 | MS.gene73596 | 0.800232 | 1.59E-48 | -1.69E-46 |
| MS.gene050750 | MS.gene73596 | 0.837985 | 3.76E-57 | -1.69E-46 |
| MS.gene050780 | MS.gene73596 | 0.800023 | 1.75E-48 | -1.69E-46 |
| MS.gene050844 | MS.gene73596 | 0.807169 | 5.76E-50 | -1.69E-46 |
| MS.gene050953 | MS.gene73596 | 0.837067 | 6.47E-57 | -1.69E-46 |
| MS.gene051368 | MS.gene73596 | 0.81485 | 1.25E-51 | -1.69E-46 |
| MS.gene051594 | MS.gene73596 | 0.81104 | 8.54E-51 | -1.69E-46 |
| MS.gene052176 | MS.gene73596 | 0.850302 | 1.85E-60 | -1.69E-46 |
| MS.gene052190 | MS.gene73596 | 0.814583 | 1.43E-51 | -1.69E-46 |
| MS.gene052208 | MS.gene73596 | 0.833769 | 4.41E-56 | -1.69E-46 |
| MS.gene052333 | MS.gene73596 | 0.839995 | 1.13E-57 | -1.69E-46 |
| MS.gene052500 | MS.gene73596 | 0.83321 | 6.08E-56 | -1.69E-46 |
| MS.gene052502 | MS.gene73596 | 0.836965 | 6.87E-57 | -1.69E-46 |
| MS.gene052519 | MS.gene73596 | 0.812074 | 5.09E-51 | -1.69E-46 |
| MS.gene052582 | MS.gene73596 | 0.810237 | 1.27E-50 | -1.69E-46 |
| MS.gene052584 | MS.gene73596 | 0.802215 | 6.23E-49 | -1.69E-46 |
| MS.gene052586 | MS.gene73596 | 0.846986 | 1.54E-59 | -1.69E-46 |
| MS.gene052824 | MS.gene73596 | 0.825725 | 4.01E-54 | -1.69E-46 |
| MS.gene052826 | MS.gene73596 | 0.834638 | 2.67E-56 | -1.69E-46 |
| MS.gene052866 | MS.gene73596 | 0.804961 | 1.68E-49 | -1.69E-46 |
| MS.gene052867 | MS.gene73596 | 0.821597 | 3.71E-53 | -1.69E-46 |
| MS.gene052954 | MS.gene73596 | 0.827532 | 1.49E-54 | -1.69E-46 |
| MS.gene053006 | MS.gene73596 | 0.850506 | 1.62E-60 | -1.69E-46 |
| MS.gene053166 | MS.gene73596 | 0.840076 | 1.08E-57 | -1.69E-46 |
| MS.gene053188 | MS.gene73596 | 0.800209 | 1.60E-48 | -1.69E-46 |
| MS.gene053216 | MS.gene73596 | 0.800705 | 1.27E-48 | -1.69E-46 |
| MS.gene053226 | MS.gene73596 | 0.811861 | 5.67E-51 | -1.69E-46 |
| MS.gene053995 | MS.gene73596 | 0.810721 | 1.00E-50 | -1.69E-46 |
| MS.gene054083 | MS.gene73596 | 0.807158 | 5.80E-50 | -1.69E-46 |
| MS.gene05410 | MS.gene73596 | 0.80248 | 5.50E-49 | -1.69E-46 |
| MS.gene05414 | MS.gene73596 | 0.808961 | 2.39E-50 | -1.69E-46 |
| MS.gene05415 | MS.gene73596 | 0.832262 | 1.05E-55 | -1.69E-46 |
| MS.gene054160 | MS.gene73596 | 0.833024 | 6.76E-56 | -1.69E-46 |
| MS.gene054236 | MS.gene73596 | 0.820957 | 5.21E-53 | -1.69E-46 |
| MS.gene054405 | MS.gene73596 | 0.828602 | 8.20E-55 | -1.69E-46 |
| MS.gene054468 | MS.gene73596 | 0.813821 | 2.11E-51 | -1.69E-46 |
| MS.gene054559 | MS.gene73596 | 0.823898 | 1.08E-53 | -1.69E-46 |
| MS.gene054576 | MS.gene73596 | 0.80454 | 2.06E-49 | -1.69E-46 |
| MS.gene054651 | MS.gene73596 | 0.808708 | 2.71E-50 | -1.69E-46 |
| MS.gene05510 | MS.gene73596 | 0.808994 | 2.36E-50 | -1.69E-46 |
| MS.gene055225 | MS.gene73596 | 0.800836 | 1.20E-48 | -1.69E-46 |
| MS.gene055586 | MS.gene73596 | 0.821234 | 4.50E-53 | -1.69E-46 |
| MS.gene055701 | MS.gene73596 | 0.845487 | 3.94E-59 | -1.69E-46 |
| MS.gene055778 | MS.gene73596 | 0.80681 | 6.87E-50 | -1.69E-46 |
| MS.gene055818 | MS.gene73596 | 0.820948 | 5.24E-53 | -1.69E-46 |
| MS.gene055841 | MS.gene73596 | 0.835667 | 1.47E-56 | -1.69E-46 |
| MS.gene056162 | MS.gene73596 | 0.804126 | 2.51E-49 | -1.69E-46 |
| MS.gene056366 | MS.gene73596 | 0.808611 | 2.84E-50 | -1.69E-46 |
| MS.gene056506 | MS.gene73596 | 0.844558 | 7.02E-59 | -1.69E-46 |
| MS.gene05659 | MS.gene73596 | 0.814073 | 1.85E-51 | -1.69E-46 |
| MS.gene057012 | MS.gene73596 | 0.814808 | 1.28E-51 | -1.69E-46 |
| MS.gene057026 | MS.gene73596 | 0.854144 | 1.50E-61 | -1.69E-46 |
| MS.gene057060 | MS.gene73596 | 0.817934 | 2.55E-52 | -1.69E-46 |
| MS.gene057208 | MS.gene73596 | 0.819682 | 1.02E-52 | -1.69E-46 |
| MS.gene057414 | MS.gene73596 | 0.804392 | 2.21E-49 | -1.69E-46 |
| MS.gene057693 | MS.gene73596 | 0.810213 | 1.29E-50 | -1.69E-46 |
| MS.gene05795 | MS.gene73596 | 0.808815 | 2.57E-50 | -1.69E-46 |
| MS.gene058037 | MS.gene73596 | 0.806532 | 7.86E-50 | -1.69E-46 |
| MS.gene058548 | MS.gene73596 | 0.801238 | 9.89E-49 | -1.69E-46 |
| MS.gene058979 | MS.gene73596 | 0.824324 | 8.59E-54 | -1.69E-46 |
| MS.gene059317 | MS.gene73596 | 0.800788 | 1.22E-48 | -1.69E-46 |
| MS.gene059382 | MS.gene73596 | 0.829644 | 4.59E-55 | -1.69E-46 |
| MS.gene05954 | MS.gene73596 | 0.815765 | 7.82E-52 | -1.69E-46 |
| MS.gene059830 | MS.gene73596 | 0.821585 | 3.73E-53 | -1.69E-46 |
| MS.gene060011 | MS.gene73596 | 0.802523 | 5.39E-49 | -1.69E-46 |
| MS.gene060322 | MS.gene73596 | 0.809319 | 2.01E-50 | -1.69E-46 |
| MS.gene060521 | MS.gene73596 | 0.801051 | 1.08E-48 | -1.69E-46 |
| MS.gene060572 | MS.gene73596 | 0.835806 | 1.35E-56 | -1.69E-46 |
| MS.gene060879 | MS.gene73596 | 0.841974 | 3.43E-58 | -1.69E-46 |
| MS.gene060998 | MS.gene73596 | 0.801622 | 8.25E-49 | -1.69E-46 |
| MS.gene061040 | MS.gene73596 | -0.860133 | 2.55E-63 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73596.t1 | MTR_7g027300 | 87.611 | 113 | 14 | 0 | 1 | 113 | 1 | 113 | 2.14e-70 | 207 |
| MS.gene73596.t1 | MTR_7g027017 | 93.396 | 106 | 7 | 0 | 1 | 106 | 1 | 106 | 3.44e-70 | 213 |
| MS.gene73596.t1 | MTR_8g096380 | 57.895 | 114 | 48 | 0 | 1 | 114 | 1 | 114 | 4.66e-49 | 153 |
| MS.gene73596.t1 | MTR_8g096390 | 51.887 | 106 | 49 | 1 | 1 | 104 | 1 | 106 | 1.08e-36 | 122 |
| MS.gene73596.t1 | MTR_7g012560 | 56.989 | 93 | 38 | 1 | 3 | 93 | 4 | 96 | 6.52e-34 | 114 |
| MS.gene73596.t1 | MTR_7g012560 | 56.989 | 93 | 38 | 1 | 3 | 93 | 4 | 96 | 6.87e-34 | 115 |
| MS.gene73596.t1 | MTR_6g013820 | 57.471 | 87 | 37 | 0 | 7 | 93 | 8 | 94 | 1.06e-32 | 112 |
| MS.gene73596.t1 | MTR_7g108180 | 52.041 | 98 | 45 | 2 | 4 | 100 | 5 | 101 | 4.66e-32 | 110 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73596.t1 | AT3G26980 | 72.115 | 104 | 29 | 0 | 1 | 104 | 1 | 104 | 4.83e-54 | 166 |
| MS.gene73596.t1 | AT4G24990 | 56.780 | 118 | 50 | 1 | 1 | 118 | 1 | 117 | 9.48e-48 | 150 |
| MS.gene73596.t1 | AT4G24990 | 56.780 | 118 | 50 | 1 | 1 | 118 | 1 | 117 | 9.48e-48 | 150 |
| MS.gene73596.t1 | AT1G22050 | 53.125 | 96 | 44 | 1 | 3 | 97 | 4 | 99 | 3.74e-34 | 115 |
| MS.gene73596.t1 | AT1G77870 | 55.446 | 101 | 43 | 1 | 1 | 99 | 1 | 101 | 6.09e-34 | 115 |
| MS.gene73596.t1 | AT5G15460 | 54.651 | 86 | 39 | 0 | 7 | 92 | 8 | 93 | 4.12e-31 | 108 |
| MS.gene73596.t1 | AT5G15460 | 54.651 | 86 | 39 | 0 | 7 | 92 | 8 | 93 | 4.12e-31 | 108 |
| MS.gene73596.t1 | AT5G15460 | 54.651 | 86 | 39 | 0 | 7 | 92 | 8 | 93 | 4.12e-31 | 108 |
| MS.gene73596.t1 | AT3G01050 | 50.000 | 86 | 43 | 0 | 7 | 92 | 8 | 93 | 7.48e-27 | 97.1 |
| MS.gene73596.t1 | AT3G01050 | 50.000 | 86 | 43 | 0 | 7 | 92 | 8 | 93 | 7.48e-27 | 97.1 |
| MS.gene73596.t1 | AT3G01050 | 50.000 | 86 | 43 | 0 | 7 | 92 | 8 | 93 | 7.48e-27 | 97.1 |
| MS.gene73596.t1 | AT3G01050 | 50.000 | 86 | 43 | 0 | 7 | 92 | 8 | 93 | 7.48e-27 | 97.1 |
| MS.gene73596.t1 | AT3G01050 | 50.000 | 86 | 43 | 0 | 7 | 92 | 8 | 93 | 7.48e-27 | 97.1 |
| MS.gene73596.t1 | AT5G15460 | 54.902 | 51 | 23 | 0 | 42 | 92 | 17 | 67 | 1.72e-13 | 62.4 |
Find 36 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATAAATGCTGGCAAAATTT+TGG | 0.148008 | 7.1:+70194588 | MS.gene73596:CDS |
| TGTTGTCGTTCAGCCATCTT+TGG | 0.348360 | 7.1:+70194681 | MS.gene73596:CDS |
| TATCTGATCCATCGTATATA+CGG | 0.369078 | 7.1:-70191655 | None:intergenic |
| ATATACGATGGATCAGATAT+TGG | 0.380125 | 7.1:+70191659 | MS.gene73596:CDS |
| CACACCAAAGTTACTCAATC+TGG | 0.382608 | 7.1:-70197515 | None:intergenic |
| GGCCAATGTAGAGTACCTTT+TGG | 0.405269 | 7.1:+70194628 | MS.gene73596:CDS |
| TTCGATTGATCGGATGGTTA+AGG | 0.405967 | 7.1:-70197554 | None:intergenic |
| TGAAGTTGGTGAATAAGAAA+AGG | 0.430837 | 7.1:-70191684 | None:intergenic |
| AGCTCCACCAATTCATCATC+AGG | 0.438663 | 7.1:-70191626 | None:intergenic |
| CGAGATGCCTGATGATGAAT+TGG | 0.447255 | 7.1:+70191619 | None:intergenic |
| TATTCGACTCGTGCGGCGCA+CGG | 0.448387 | 7.1:+70202067 | MS.gene73596:CDS |
| TTAATATCACTTGCTGCCCT+TGG | 0.457315 | 7.1:-70194562 | None:intergenic |
| GGATCCAGATTGAGTAACTT+TGG | 0.465645 | 7.1:+70197511 | MS.gene73596:intron |
| AGCATAGAAACAGTTGAAGT+TGG | 0.470657 | 7.1:-70191698 | None:intergenic |
| TCTTTGGCAAAAGCGAAAAC+AGG | 0.475441 | 7.1:+70194697 | MS.gene73596:CDS |
| ATTGGGTACCTTCGATTGAT+CGG | 0.500713 | 7.1:-70197564 | None:intergenic |
| TTCAACTGTTTCTATGCTCA+AGG | 0.504011 | 7.1:+70191703 | MS.gene73596:CDS |
| ATTCGACTCGTGCGGCGCAC+GGG | 0.504669 | 7.1:+70202068 | MS.gene73596:CDS |
| GATATTAAACTCATAAATGC+TGG | 0.505519 | 7.1:+70194577 | MS.gene73596:CDS |
| GAAGTTGGTGAATAAGAAAA+GGG | 0.509171 | 7.1:-70191683 | None:intergenic |
| GGTACCTTCGATTGATCGGA+TGG | 0.527392 | 7.1:-70197560 | None:intergenic |
| CGCCAAAAGGTACTCTACAT+TGG | 0.536293 | 7.1:-70194630 | None:intergenic |
| CCAGAAGGAAGCTCGCCAAA+AGG | 0.538717 | 7.1:-70194643 | None:intergenic |
| AGATTGAGTAACTTTGGTGT+GGG | 0.566311 | 7.1:+70197517 | MS.gene73596:CDS |
| CAGATTGAGTAACTTTGGTG+TGG | 0.574769 | 7.1:+70197516 | MS.gene73596:CDS |
| CTGTTTCTATGCTCAAGGAA+AGG | 0.586323 | 7.1:+70191708 | MS.gene73596:CDS |
| TGCATGGTGATGACCCCAGA+AGG | 0.596413 | 7.1:-70194658 | None:intergenic |
| TTGGAGAACAACAAGACGGT+GGG | 0.608984 | 7.1:+70194607 | MS.gene73596:CDS |
| GATGCCTGATGATGAATTGG+TGG | 0.609921 | 7.1:+70191622 | None:intergenic |
| AGACAAGAAAATTATACCAA+GGG | 0.613126 | 7.1:+70194546 | MS.gene73596:intron |
| CTGAAGTTCCGTATATACGA+TGG | 0.627299 | 7.1:+70191647 | MS.gene73596:CDS |
| CAGACAAGAAAATTATACCA+AGG | 0.653948 | 7.1:+70194545 | MS.gene73596:intron |
| TTAACCATCCGATCAATCGA+AGG | 0.663385 | 7.1:+70197556 | MS.gene73596:CDS |
| TTTGGAGAACAACAAGACGG+TGG | 0.663548 | 7.1:+70194606 | MS.gene73596:CDS |
| TGGCTGAACGACAACATGCA+TGG | 0.679504 | 7.1:-70194674 | None:intergenic |
| TACATTCTATTCGACTCGTG+CGG | 0.715500 | 7.1:+70202060 | MS.gene73596:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 70191623 | 70202106 | 70191623 | ID=MS.gene73596 |
| chr7.1 | mRNA | 70191623 | 70202106 | 70191623 | ID=MS.gene73596.t1;Parent=MS.gene73596 |
| chr7.1 | exon | 70191623 | 70191752 | 70191623 | ID=MS.gene73596.t1.exon1;Parent=MS.gene73596.t1 |
| chr7.1 | CDS | 70191623 | 70191752 | 70191623 | ID=cds.MS.gene73596.t1;Parent=MS.gene73596.t1 |
| chr7.1 | exon | 70194548 | 70194718 | 70194548 | ID=MS.gene73596.t1.exon2;Parent=MS.gene73596.t1 |
| chr7.1 | CDS | 70194548 | 70194718 | 70194548 | ID=cds.MS.gene73596.t1;Parent=MS.gene73596.t1 |
| chr7.1 | exon | 70197512 | 70197577 | 70197512 | ID=MS.gene73596.t1.exon3;Parent=MS.gene73596.t1 |
| chr7.1 | CDS | 70197512 | 70197577 | 70197512 | ID=cds.MS.gene73596.t1;Parent=MS.gene73596.t1 |
| chr7.1 | exon | 70202060 | 70202106 | 70202060 | ID=MS.gene73596.t1.exon4;Parent=MS.gene73596.t1 |
| chr7.1 | CDS | 70202060 | 70202106 | 70202060 | ID=cds.MS.gene73596.t1;Parent=MS.gene73596.t1 |
| Gene Sequence |
| Protein sequence |
