Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44603.t1 | XP_013452335.1 | 71.8 | 124 | 31 | 1 | 1 | 124 | 235 | 354 | 6.10E-36 | 160.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44603.t1 | Q8LG95 | 66.1 | 56 | 19 | 0 | 1 | 56 | 188 | 243 | 2.3e-15 | 83.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44603.t1 | A0A072UBE9 | 71.8 | 124 | 31 | 1 | 1 | 124 | 235 | 354 | 4.4e-36 | 160.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049159 | MS.gene44603 | 0.82123 | 4.51E-53 | -1.69E-46 |
| MS.gene049230 | MS.gene44603 | 0.809272 | 2.05E-50 | -1.69E-46 |
| MS.gene049254 | MS.gene44603 | 0.810044 | 1.40E-50 | -1.69E-46 |
| MS.gene049297 | MS.gene44603 | 0.814983 | 1.17E-51 | -1.69E-46 |
| MS.gene049378 | MS.gene44603 | 0.857527 | 1.54E-62 | -1.69E-46 |
| MS.gene049404 | MS.gene44603 | 0.805427 | 1.34E-49 | -1.69E-46 |
| MS.gene049412 | MS.gene44603 | 0.808361 | 3.22E-50 | -1.69E-46 |
| MS.gene049424 | MS.gene44603 | 0.804853 | 1.77E-49 | -1.69E-46 |
| MS.gene049435 | MS.gene44603 | 0.812351 | 4.43E-51 | -1.69E-46 |
| MS.gene049669 | MS.gene44603 | 0.827693 | 1.36E-54 | -1.69E-46 |
| MS.gene049821 | MS.gene44603 | 0.806001 | 1.02E-49 | -1.69E-46 |
| MS.gene049877 | MS.gene44603 | 0.813259 | 2.80E-51 | -1.69E-46 |
| MS.gene050026 | MS.gene44603 | 0.808761 | 2.64E-50 | -1.69E-46 |
| MS.gene05016 | MS.gene44603 | 0.803003 | 4.29E-49 | -1.69E-46 |
| MS.gene050201 | MS.gene44603 | 0.80131 | 9.56E-49 | -1.69E-46 |
| MS.gene050226 | MS.gene44603 | 0.857664 | 1.40E-62 | -1.69E-46 |
| MS.gene050227 | MS.gene44603 | 0.864014 | 1.65E-64 | -1.69E-46 |
| MS.gene050228 | MS.gene44603 | 0.870466 | 1.43E-66 | -1.69E-46 |
| MS.gene050237 | MS.gene44603 | 0.854996 | 8.48E-62 | -1.69E-46 |
| MS.gene050330 | MS.gene44603 | -0.814511 | 1.48E-51 | -1.69E-46 |
| MS.gene050442 | MS.gene44603 | 0.849308 | 3.51E-60 | -1.69E-46 |
| MS.gene050623 | MS.gene44603 | -0.829877 | 4.03E-55 | -1.69E-46 |
| MS.gene050680 | MS.gene44603 | 0.822653 | 2.11E-53 | -1.69E-46 |
| MS.gene050719 | MS.gene44603 | 0.842412 | 2.63E-58 | -1.69E-46 |
| MS.gene050721 | MS.gene44603 | 0.842029 | 3.32E-58 | -1.69E-46 |
| MS.gene050724 | MS.gene44603 | 0.818629 | 1.77E-52 | -1.69E-46 |
| MS.gene050750 | MS.gene44603 | 0.836863 | 7.29E-57 | -1.69E-46 |
| MS.gene050780 | MS.gene44603 | 0.859315 | 4.50E-63 | -1.69E-46 |
| MS.gene050807 | MS.gene44603 | 0.824376 | 8.35E-54 | -1.69E-46 |
| MS.gene050820 | MS.gene44603 | 0.80803 | 3.78E-50 | -1.69E-46 |
| MS.gene050824 | MS.gene44603 | 0.81578 | 7.76E-52 | -1.69E-46 |
| MS.gene050953 | MS.gene44603 | 0.848418 | 6.21E-60 | -1.69E-46 |
| MS.gene050987 | MS.gene44603 | 0.808975 | 2.38E-50 | -1.69E-46 |
| MS.gene051198 | MS.gene44603 | 0.805966 | 1.03E-49 | -1.69E-46 |
| MS.gene051210 | MS.gene44603 | 0.807252 | 5.54E-50 | -1.69E-46 |
| MS.gene051265 | MS.gene44603 | 0.804685 | 1.92E-49 | -1.69E-46 |
| MS.gene051349 | MS.gene44603 | 0.815118 | 1.09E-51 | -1.69E-46 |
| MS.gene051370 | MS.gene44603 | 0.803459 | 3.45E-49 | -1.69E-46 |
| MS.gene051744 | MS.gene44603 | 0.802228 | 6.20E-49 | -1.69E-46 |
| MS.gene051983 | MS.gene44603 | 0.825957 | 3.53E-54 | -1.69E-46 |
| MS.gene052108 | MS.gene44603 | 0.822405 | 2.41E-53 | -1.69E-46 |
| MS.gene052176 | MS.gene44603 | 0.848296 | 6.71E-60 | -1.69E-46 |
| MS.gene052190 | MS.gene44603 | 0.803204 | 3.90E-49 | -1.69E-46 |
| MS.gene052208 | MS.gene44603 | 0.800628 | 1.32E-48 | -1.69E-46 |
| MS.gene052286 | MS.gene44603 | 0.8018 | 7.59E-49 | -1.69E-46 |
| MS.gene052302 | MS.gene44603 | 0.810426 | 1.16E-50 | -1.69E-46 |
| MS.gene052309 | MS.gene44603 | -0.806276 | 8.90E-50 | -1.69E-46 |
| MS.gene052323 | MS.gene44603 | 0.821764 | 3.39E-53 | -1.69E-46 |
| MS.gene052333 | MS.gene44603 | 0.802661 | 5.05E-49 | -1.69E-46 |
| MS.gene052358 | MS.gene44603 | -0.803502 | 3.38E-49 | -1.69E-46 |
| MS.gene052376 | MS.gene44603 | 0.823146 | 1.62E-53 | -1.69E-46 |
| MS.gene052384 | MS.gene44603 | 0.808171 | 3.53E-50 | -1.69E-46 |
| MS.gene052426 | MS.gene44603 | 0.813966 | 1.96E-51 | -1.69E-46 |
| MS.gene052519 | MS.gene44603 | 0.806864 | 6.69E-50 | -1.69E-46 |
| MS.gene052586 | MS.gene44603 | 0.802151 | 6.43E-49 | -1.69E-46 |
| MS.gene052856 | MS.gene44603 | 0.800212 | 1.60E-48 | -1.69E-46 |
| MS.gene052858 | MS.gene44603 | 0.805266 | 1.45E-49 | -1.69E-46 |
| MS.gene052860 | MS.gene44603 | 0.822645 | 2.12E-53 | -1.69E-46 |
| MS.gene052863 | MS.gene44603 | 0.801988 | 6.94E-49 | -1.69E-46 |
| MS.gene052866 | MS.gene44603 | 0.800127 | 1.67E-48 | -1.69E-46 |
| MS.gene052867 | MS.gene44603 | 0.823848 | 1.11E-53 | -1.69E-46 |
| MS.gene053006 | MS.gene44603 | 0.832067 | 1.17E-55 | -1.69E-46 |
| MS.gene053085 | MS.gene44603 | 0.851824 | 6.90E-61 | -1.69E-46 |
| MS.gene053166 | MS.gene44603 | 0.82738 | 1.61E-54 | -1.69E-46 |
| MS.gene053201 | MS.gene44603 | 0.815925 | 7.20E-52 | -1.69E-46 |
| MS.gene053216 | MS.gene44603 | 0.815833 | 7.55E-52 | -1.69E-46 |
| MS.gene053565 | MS.gene44603 | 0.814559 | 1.45E-51 | -1.69E-46 |
| MS.gene053630 | MS.gene44603 | 0.807014 | 6.22E-50 | -1.69E-46 |
| MS.gene054083 | MS.gene44603 | 0.829299 | 5.56E-55 | -1.69E-46 |
| MS.gene05410 | MS.gene44603 | 0.808229 | 3.43E-50 | -1.69E-46 |
| MS.gene05414 | MS.gene44603 | 0.83736 | 5.44E-57 | -1.69E-46 |
| MS.gene05415 | MS.gene44603 | 0.87844 | 2.80E-69 | -1.69E-46 |
| MS.gene054160 | MS.gene44603 | 0.818541 | 1.86E-52 | -1.69E-46 |
| MS.gene054236 | MS.gene44603 | 0.814177 | 1.76E-51 | -1.69E-46 |
| MS.gene054291 | MS.gene44603 | -0.818633 | 1.77E-52 | -1.69E-46 |
| MS.gene054405 | MS.gene44603 | 0.834055 | 3.74E-56 | -1.69E-46 |
| MS.gene054468 | MS.gene44603 | 0.806665 | 7.37E-50 | -1.69E-46 |
| MS.gene054472 | MS.gene44603 | 0.805469 | 1.32E-49 | -1.69E-46 |
| MS.gene054559 | MS.gene44603 | 0.806158 | 9.42E-50 | -1.69E-46 |
| MS.gene054657 | MS.gene44603 | 0.823224 | 1.55E-53 | -1.69E-46 |
| MS.gene054930 | MS.gene44603 | 0.830421 | 2.96E-55 | -1.69E-46 |
| MS.gene054942 | MS.gene44603 | 0.824808 | 6.60E-54 | -1.69E-46 |
| MS.gene05510 | MS.gene44603 | 0.823196 | 1.58E-53 | -1.69E-46 |
| MS.gene055227 | MS.gene44603 | 0.800981 | 1.12E-48 | -1.69E-46 |
| MS.gene055228 | MS.gene44603 | 0.834246 | 3.35E-56 | -1.69E-46 |
| MS.gene055357 | MS.gene44603 | 0.801107 | 1.05E-48 | -1.69E-46 |
| MS.gene055542 | MS.gene44603 | 0.815737 | 7.93E-52 | -1.69E-46 |
| MS.gene055623 | MS.gene44603 | 0.808484 | 3.03E-50 | -1.69E-46 |
| MS.gene055701 | MS.gene44603 | 0.838327 | 3.07E-57 | -1.69E-46 |
| MS.gene055711 | MS.gene44603 | 0.837016 | 6.67E-57 | -1.69E-46 |
| MS.gene055778 | MS.gene44603 | 0.80077 | 1.23E-48 | -1.69E-46 |
| MS.gene055818 | MS.gene44603 | 0.847625 | 1.03E-59 | -1.69E-46 |
| MS.gene05604 | MS.gene44603 | 0.807309 | 5.38E-50 | -1.69E-46 |
| MS.gene056366 | MS.gene44603 | 0.803404 | 3.54E-49 | -1.69E-46 |
| MS.gene056498 | MS.gene44603 | 0.811472 | 6.88E-51 | -1.69E-46 |
| MS.gene056506 | MS.gene44603 | 0.84965 | 2.82E-60 | -1.69E-46 |
| MS.gene05659 | MS.gene44603 | 0.805825 | 1.11E-49 | -1.69E-46 |
| MS.gene056760 | MS.gene44603 | 0.812717 | 3.68E-51 | -1.69E-46 |
| MS.gene056873 | MS.gene44603 | 0.821294 | 4.36E-53 | -1.69E-46 |
| MS.gene056979 | MS.gene44603 | 0.820832 | 5.57E-53 | -1.69E-46 |
| MS.gene057021 | MS.gene44603 | 0.813859 | 2.07E-51 | -1.69E-46 |
| MS.gene057026 | MS.gene44603 | 0.834346 | 3.16E-56 | -1.69E-46 |
| MS.gene057060 | MS.gene44603 | 0.862517 | 4.79E-64 | -1.69E-46 |
| MS.gene057079 | MS.gene44603 | 0.812074 | 5.09E-51 | -1.69E-46 |
| MS.gene057200 | MS.gene44603 | 0.823594 | 1.27E-53 | -1.69E-46 |
| MS.gene057208 | MS.gene44603 | 0.80505 | 1.61E-49 | -1.69E-46 |
| MS.gene057414 | MS.gene44603 | 0.812016 | 5.24E-51 | -1.69E-46 |
| MS.gene057444 | MS.gene44603 | 0.82151 | 3.89E-53 | -1.69E-46 |
| MS.gene05761 | MS.gene44603 | 0.815791 | 7.71E-52 | -1.69E-46 |
| MS.gene057693 | MS.gene44603 | 0.803153 | 3.99E-49 | -1.69E-46 |
| MS.gene057869 | MS.gene44603 | 0.849447 | 3.21E-60 | -1.69E-46 |
| MS.gene05795 | MS.gene44603 | 0.844684 | 6.49E-59 | -1.69E-46 |
| MS.gene058037 | MS.gene44603 | 0.809927 | 1.49E-50 | -1.69E-46 |
| MS.gene058146 | MS.gene44603 | 0.822311 | 2.54E-53 | -1.69E-46 |
| MS.gene058399 | MS.gene44603 | 0.803328 | 3.67E-49 | -1.69E-46 |
| MS.gene058636 | MS.gene44603 | 0.818857 | 1.57E-52 | -1.69E-46 |
| MS.gene058979 | MS.gene44603 | 0.843253 | 1.57E-58 | -1.69E-46 |
| MS.gene059128 | MS.gene44603 | 0.836371 | 9.73E-57 | -1.69E-46 |
| MS.gene059161 | MS.gene44603 | 0.812963 | 3.25E-51 | -1.69E-46 |
| MS.gene059306 | MS.gene44603 | 0.818758 | 1.66E-52 | -1.69E-46 |
| MS.gene059317 | MS.gene44603 | 0.804565 | 2.03E-49 | -1.69E-46 |
| MS.gene059382 | MS.gene44603 | 0.819694 | 1.02E-52 | -1.69E-46 |
| MS.gene05954 | MS.gene44603 | 0.803262 | 3.79E-49 | -1.69E-46 |
| MS.gene059584 | MS.gene44603 | 0.810175 | 1.31E-50 | -1.69E-46 |
| MS.gene05973 | MS.gene44603 | 0.810362 | 1.20E-50 | -1.69E-46 |
| MS.gene060011 | MS.gene44603 | 0.807587 | 4.70E-50 | -1.69E-46 |
| MS.gene060137 | MS.gene44603 | -0.812894 | 3.37E-51 | -1.69E-46 |
| MS.gene060297 | MS.gene44603 | 0.804468 | 2.13E-49 | -1.69E-46 |
| MS.gene060310 | MS.gene44603 | 0.813791 | 2.14E-51 | -1.69E-46 |
| MS.gene060322 | MS.gene44603 | 0.835486 | 1.63E-56 | -1.69E-46 |
| MS.gene060480 | MS.gene44603 | 0.814533 | 1.47E-51 | -1.69E-46 |
| MS.gene060521 | MS.gene44603 | 0.816317 | 5.89E-52 | -1.69E-46 |
| MS.gene06052 | MS.gene44603 | 0.800615 | 1.33E-48 | -1.69E-46 |
| MS.gene060572 | MS.gene44603 | 0.850311 | 1.84E-60 | -1.69E-46 |
| MS.gene060634 | MS.gene44603 | -0.832122 | 1.13E-55 | -1.69E-46 |
| MS.gene060683 | MS.gene44603 | 0.857992 | 1.12E-62 | -1.69E-46 |
| MS.gene060685 | MS.gene44603 | 0.858021 | 1.10E-62 | -1.69E-46 |
| MS.gene060879 | MS.gene44603 | 0.812102 | 5.02E-51 | -1.69E-46 |
| MS.gene060918 | MS.gene44603 | 0.836021 | 1.19E-56 | -1.69E-46 |
| MS.gene060998 | MS.gene44603 | 0.829149 | 6.05E-55 | -1.69E-46 |
| MS.gene061040 | MS.gene44603 | -0.835651 | 1.48E-56 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44603.t1 | MTR_6g053090 | 90.323 | 124 | 8 | 1 | 1 | 124 | 235 | 354 | 9.36e-66 | 202 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44603.t1 | AT4G21190 | 66.071 | 56 | 19 | 0 | 1 | 56 | 188 | 243 | 2.14e-21 | 87.0 |
Find 0 sgRNAs with CRISPR-Local
Find 26 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ACGTATCTATCAACTTTATT+AGG | - | 43123:163540-163559 | None:intergenic | 25.0% |
| ATGTATCTATATTCCCATTG+TGG | - | 43123:163480-163499 | None:intergenic | 30.0% | |
| TGGGAATATAGATACATCAA+AGG | + | 43123:163483-163502 | MS.gene44603:CDS | 30.0% | |
| ! | TGACTCATCTGAAATTTGTT+TGG | - | 43123:163685-163704 | None:intergenic | 30.0% |
| AAAAATATCCACCACCACAA+TGG | + | 43123:163463-163482 | MS.gene44603:CDS | 35.0% | |
| AAAATATCCACCACCACAAT+GGG | + | 43123:163464-163483 | MS.gene44603:CDS | 35.0% | |
| AATGGTTGGAAATGTCTTCA+AGG | + | 43123:163404-163423 | MS.gene44603:CDS | 35.0% | |
| AGAAGAGACTTCAGAGTTAA+CGG | + | 43123:163614-163633 | MS.gene44603:CDS | 35.0% | |
| AGATGAGTCATCTGAGTTTA+CGG | + | 43123:163695-163714 | MS.gene44603:CDS | 35.0% | |
| ATTGAGACAACAGAACTACT+AGG | - | 43123:163387-163406 | None:intergenic | 35.0% | |
| GACAATGTTCAACGAAATTC+AGG | + | 43123:163570-163589 | MS.gene44603:CDS | 35.0% | |
| GGTTTCCATGCCTATTAAAT+CGG | - | 43123:163664-163683 | None:intergenic | 35.0% | |
| TATCTATATTCCCATTGTGG+TGG | - | 43123:163477-163496 | None:intergenic | 35.0% | |
| ! | TAGTAGTTCTGTTGTCTCAA+TGG | + | 43123:163386-163405 | MS.gene44603:CDS | 35.0% |
| ACGAAATTCAGGCTTAAGAG+AGG | + | 43123:163581-163600 | MS.gene44603:CDS | 40.0% | |
| AGTTCTGTTGTCTCAATGGT+TGG | + | 43123:163390-163409 | MS.gene44603:CDS | 40.0% | |
| GAAGAGACTTCAGAGTTAAC+GGG | + | 43123:163615-163634 | MS.gene44603:CDS | 40.0% | |
| ! | AAGGAAAGCGTGTAAGAATC+AGG | + | 43123:163502-163521 | MS.gene44603:CDS | 40.0% |
| ! | AGGAAAGCGTGTAAGAATCA+GGG | + | 43123:163503-163522 | MS.gene44603:CDS | 40.0% |
| !! | GAACAGGATGCCGATTTAAT+AGG | + | 43123:163651-163670 | MS.gene44603:CDS | 40.0% |
| CTATATTCCCATTGTGGTGG+TGG | - | 43123:163474-163493 | None:intergenic | 45.0% | |
| CTTCAAGGAGCTAGGTATGA+TGG | + | 43123:163419-163438 | MS.gene44603:CDS | 45.0% | |
| GGAAATGTCTTCAAGGAGCT+AGG | + | 43123:163411-163430 | MS.gene44603:CDS | 45.0% | |
| !! | GGATGCCGATTTAATAGGCA+TGG | + | 43123:163656-163675 | MS.gene44603:CDS | 45.0% |
| CGTGTAAGAATCAGGGTGCA+AGG | + | 43123:163510-163529 | MS.gene44603:CDS | 50.0% | |
| GGGCGATGAACAATTCGAAC+AGG | + | 43123:163635-163654 | MS.gene44603:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 43123 | gene | 163363 | 163737 | 163363 | ID=MS.gene44603 |
| 43123 | mRNA | 163363 | 163737 | 163363 | ID=MS.gene44603.t1;Parent=MS.gene44603 |
| 43123 | exon | 163363 | 163737 | 163363 | ID=MS.gene44603.t1.exon1;Parent=MS.gene44603.t1 |
| 43123 | CDS | 163363 | 163737 | 163363 | ID=cds.MS.gene44603.t1;Parent=MS.gene44603.t1 |
| Gene Sequence |
| Protein sequence |
