Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88887.t1 | XP_003592044.1 | 99.7 | 389 | 1 | 0 | 1 | 389 | 1 | 389 | 1.10E-221 | 778.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88887.t1 | P51078 | 99.2 | 389 | 3 | 0 | 1 | 389 | 1 | 389 | 1.9e-222 | 772.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88887.t1 | G7IAK9 | 99.7 | 389 | 1 | 0 | 1 | 389 | 1 | 389 | 8.1e-222 | 778.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049320 | MS.gene88887 | -0.800614 | 1.33E-48 | -1.69E-46 |
| MS.gene05015 | MS.gene88887 | 0.825474 | 4.60E-54 | -1.69E-46 |
| MS.gene050216 | MS.gene88887 | -0.804302 | 2.31E-49 | -1.69E-46 |
| MS.gene050363 | MS.gene88887 | 0.801984 | 6.96E-49 | -1.69E-46 |
| MS.gene050381 | MS.gene88887 | 0.865051 | 7.82E-65 | -1.69E-46 |
| MS.gene050504 | MS.gene88887 | -0.802301 | 5.99E-49 | -1.69E-46 |
| MS.gene050612 | MS.gene88887 | -0.813504 | 2.48E-51 | -1.69E-46 |
| MS.gene050868 | MS.gene88887 | -0.811531 | 6.68E-51 | -1.69E-46 |
| MS.gene050871 | MS.gene88887 | -0.809327 | 2.00E-50 | -1.69E-46 |
| MS.gene050883 | MS.gene88887 | 0.928486 | 2.70E-92 | -1.69E-46 |
| MS.gene051044 | MS.gene88887 | 0.836311 | 1.01E-56 | -1.69E-46 |
| MS.gene051127 | MS.gene88887 | 0.851519 | 8.42E-61 | -1.69E-46 |
| MS.gene051138 | MS.gene88887 | 0.822403 | 2.41E-53 | -1.69E-46 |
| MS.gene051364 | MS.gene88887 | 0.805741 | 1.15E-49 | -1.69E-46 |
| MS.gene051410 | MS.gene88887 | 0.803422 | 3.51E-49 | -1.69E-46 |
| MS.gene05141 | MS.gene88887 | 0.81817 | 2.25E-52 | -1.69E-46 |
| MS.gene051946 | MS.gene88887 | 0.851766 | 7.17E-61 | -1.69E-46 |
| MS.gene052053 | MS.gene88887 | 0.82737 | 1.62E-54 | -1.69E-46 |
| MS.gene052482 | MS.gene88887 | -0.808412 | 3.14E-50 | -1.69E-46 |
| MS.gene052486 | MS.gene88887 | -0.800274 | 1.56E-48 | -1.69E-46 |
| MS.gene052802 | MS.gene88887 | 0.812997 | 3.20E-51 | -1.69E-46 |
| MS.gene053069 | MS.gene88887 | 0.818843 | 1.59E-52 | -1.69E-46 |
| MS.gene053420 | MS.gene88887 | 0.836831 | 7.43E-57 | -1.69E-46 |
| MS.gene053488 | MS.gene88887 | 0.818063 | 2.38E-52 | -1.69E-46 |
| MS.gene053549 | MS.gene88887 | 0.802175 | 6.35E-49 | -1.69E-46 |
| MS.gene053561 | MS.gene88887 | 0.845815 | 3.21E-59 | -1.69E-46 |
| MS.gene053826 | MS.gene88887 | 0.845643 | 3.57E-59 | -1.69E-46 |
| MS.gene054054 | MS.gene88887 | 0.803719 | 3.05E-49 | -1.69E-46 |
| MS.gene054056 | MS.gene88887 | 0.825857 | 3.73E-54 | -1.69E-46 |
| MS.gene054708 | MS.gene88887 | 0.835587 | 1.54E-56 | -1.69E-46 |
| MS.gene055124 | MS.gene88887 | 0.855215 | 7.33E-62 | -1.69E-46 |
| MS.gene055139 | MS.gene88887 | 0.826921 | 2.08E-54 | -1.69E-46 |
| MS.gene055154 | MS.gene88887 | 0.864658 | 1.04E-64 | -1.69E-46 |
| MS.gene055202 | MS.gene88887 | 0.815619 | 8.43E-52 | -1.69E-46 |
| MS.gene055502 | MS.gene88887 | -0.805142 | 1.54E-49 | -1.69E-46 |
| MS.gene055549 | MS.gene88887 | 0.838425 | 2.90E-57 | -1.69E-46 |
| MS.gene055550 | MS.gene88887 | 0.832714 | 8.07E-56 | -1.69E-46 |
| MS.gene055551 | MS.gene88887 | 0.815555 | 8.71E-52 | -1.69E-46 |
| MS.gene055942 | MS.gene88887 | 0.821131 | 4.75E-53 | -1.69E-46 |
| MS.gene055961 | MS.gene88887 | 0.807891 | 4.05E-50 | -1.69E-46 |
| MS.gene056310 | MS.gene88887 | 0.872526 | 2.97E-67 | -1.69E-46 |
| MS.gene056311 | MS.gene88887 | 0.906095 | 2.14E-80 | -1.69E-46 |
| MS.gene056313 | MS.gene88887 | 0.852227 | 5.30E-61 | -1.69E-46 |
| MS.gene056365 | MS.gene88887 | 0.802878 | 4.55E-49 | -1.69E-46 |
| MS.gene056504 | MS.gene88887 | -0.819906 | 9.08E-53 | -1.69E-46 |
| MS.gene056530 | MS.gene88887 | 0.810785 | 9.70E-51 | -1.69E-46 |
| MS.gene057014 | MS.gene88887 | 0.875109 | 3.99E-68 | -1.69E-46 |
| MS.gene058068 | MS.gene88887 | 0.80041 | 1.46E-48 | -1.69E-46 |
| MS.gene058093 | MS.gene88887 | 0.805353 | 1.39E-49 | -1.69E-46 |
| MS.gene058099 | MS.gene88887 | 0.843256 | 1.57E-58 | -1.69E-46 |
| MS.gene058108 | MS.gene88887 | 0.831821 | 1.34E-55 | -1.69E-46 |
| MS.gene058109 | MS.gene88887 | 0.885266 | 9.43E-72 | -1.69E-46 |
| MS.gene058111 | MS.gene88887 | 0.893068 | 8.86E-75 | -1.69E-46 |
| MS.gene058117 | MS.gene88887 | 0.80107 | 1.07E-48 | -1.69E-46 |
| MS.gene058171 | MS.gene88887 | 0.885938 | 5.28E-72 | -1.69E-46 |
| MS.gene058173 | MS.gene88887 | 0.888471 | 5.73E-73 | -1.69E-46 |
| MS.gene058175 | MS.gene88887 | 0.88781 | 1.03E-72 | -1.69E-46 |
| MS.gene058268 | MS.gene88887 | 0.802656 | 5.06E-49 | -1.69E-46 |
| MS.gene058493 | MS.gene88887 | 0.863098 | 3.17E-64 | -1.69E-46 |
| MS.gene058494 | MS.gene88887 | 0.870141 | 1.83E-66 | -1.69E-46 |
| MS.gene058496 | MS.gene88887 | 0.81961 | 1.06E-52 | -1.69E-46 |
| MS.gene058628 | MS.gene88887 | 0.854646 | 1.07E-61 | -1.69E-46 |
| MS.gene058629 | MS.gene88887 | 0.841868 | 3.66E-58 | -1.69E-46 |
| MS.gene058630 | MS.gene88887 | 0.847804 | 9.17E-60 | -1.69E-46 |
| MS.gene05884 | MS.gene88887 | 0.80157 | 8.46E-49 | -1.69E-46 |
| MS.gene059022 | MS.gene88887 | -0.811095 | 8.31E-51 | -1.69E-46 |
| MS.gene059037 | MS.gene88887 | 0.803509 | 3.37E-49 | -1.69E-46 |
| MS.gene059045 | MS.gene88887 | 0.818317 | 2.09E-52 | -1.69E-46 |
| MS.gene059223 | MS.gene88887 | 0.822164 | 2.74E-53 | -1.69E-46 |
| MS.gene059690 | MS.gene88887 | 0.861801 | 7.95E-64 | -1.69E-46 |
| MS.gene059691 | MS.gene88887 | 0.831872 | 1.31E-55 | -1.69E-46 |
| MS.gene059694 | MS.gene88887 | 0.865376 | 6.17E-65 | -1.69E-46 |
| MS.gene059697 | MS.gene88887 | 0.863269 | 2.81E-64 | -1.69E-46 |
| MS.gene059865 | MS.gene88887 | -0.821906 | 3.15E-53 | -1.69E-46 |
| MS.gene06077 | MS.gene88887 | -0.815001 | 1.16E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88887.t1 | MTR_1g098140 | 99.743 | 389 | 1 | 0 | 1 | 389 | 1 | 389 | 0.0 | 804 |
| MS.gene88887.t1 | MTR_1g097935 | 99.486 | 389 | 2 | 0 | 1 | 389 | 1 | 389 | 0.0 | 802 |
| MS.gene88887.t1 | MTR_1g097910 | 99.229 | 389 | 3 | 0 | 1 | 389 | 1 | 389 | 0.0 | 801 |
| MS.gene88887.t1 | MTR_7g016800 | 98.458 | 389 | 6 | 0 | 1 | 389 | 1 | 389 | 0.0 | 795 |
| MS.gene88887.t1 | MTR_7g016720 | 98.458 | 389 | 6 | 0 | 1 | 389 | 1 | 389 | 0.0 | 793 |
| MS.gene88887.t1 | MTR_7g016820 | 98.201 | 389 | 7 | 0 | 1 | 389 | 1 | 389 | 0.0 | 792 |
| MS.gene88887.t1 | MTR_5g007730 | 97.686 | 389 | 9 | 0 | 1 | 389 | 1 | 389 | 0.0 | 792 |
| MS.gene88887.t1 | MTR_7g016700 | 97.686 | 389 | 9 | 0 | 1 | 389 | 1 | 389 | 0.0 | 791 |
| MS.gene88887.t1 | MTR_5g007760 | 97.686 | 389 | 9 | 0 | 1 | 389 | 1 | 389 | 0.0 | 791 |
| MS.gene88887.t1 | MTR_7g016780 | 97.686 | 389 | 9 | 0 | 1 | 389 | 1 | 389 | 0.0 | 789 |
| MS.gene88887.t1 | MTR_5g007713 | 96.401 | 389 | 14 | 0 | 1 | 389 | 1 | 389 | 0.0 | 782 |
| MS.gene88887.t1 | MTR_5g007717 | 96.144 | 389 | 15 | 0 | 1 | 389 | 1 | 389 | 0.0 | 781 |
| MS.gene88887.t1 | MTR_3g083920 | 90.979 | 388 | 35 | 0 | 1 | 388 | 1 | 388 | 0.0 | 751 |
| MS.gene88887.t1 | MTR_3g083910 | 90.206 | 388 | 38 | 0 | 1 | 388 | 1 | 388 | 0.0 | 748 |
| MS.gene88887.t1 | MTR_2g058470 | 87.404 | 389 | 49 | 0 | 1 | 389 | 1 | 389 | 0.0 | 717 |
| MS.gene88887.t1 | MTR_3g086260 | 81.959 | 388 | 70 | 0 | 1 | 388 | 1 | 388 | 0.0 | 680 |
| MS.gene88887.t1 | MTR_7g084300 | 80.463 | 389 | 76 | 0 | 1 | 389 | 1 | 389 | 0.0 | 674 |
| MS.gene88887.t1 | MTR_7g113405 | 96.370 | 303 | 11 | 0 | 87 | 389 | 1 | 303 | 0.0 | 600 |
| MS.gene88887.t1 | MTR_3g088675 | 66.324 | 389 | 131 | 0 | 1 | 389 | 1 | 389 | 0.0 | 576 |
| MS.gene88887.t1 | MTR_5g007740 | 80.368 | 326 | 64 | 0 | 64 | 389 | 1 | 326 | 0.0 | 550 |
| MS.gene88887.t1 | MTR_5g007723 | 68.766 | 397 | 75 | 5 | 12 | 389 | 32 | 398 | 0.0 | 533 |
| MS.gene88887.t1 | MTR_5g007770 | 78.000 | 300 | 33 | 2 | 70 | 369 | 1 | 267 | 1.13e-167 | 469 |
| MS.gene88887.t1 | MTR_3g088670 | 49.231 | 390 | 184 | 6 | 10 | 388 | 2 | 388 | 6.85e-131 | 381 |
| MS.gene88887.t1 | MTR_4g075560 | 42.632 | 380 | 211 | 4 | 11 | 387 | 13 | 388 | 1.32e-102 | 309 |
| MS.gene88887.t1 | MTR_4g078730 | 37.802 | 373 | 225 | 2 | 15 | 387 | 24 | 389 | 7.77e-94 | 286 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88887.t1 | AT5G13930 | 82.732 | 388 | 66 | 1 | 3 | 389 | 8 | 395 | 0.0 | 675 |
| MS.gene88887.t1 | AT4G34850 | 40.849 | 377 | 219 | 3 | 15 | 389 | 18 | 392 | 2.05e-97 | 296 |
| MS.gene88887.t1 | AT4G00040 | 40.584 | 377 | 214 | 5 | 14 | 387 | 16 | 385 | 9.90e-96 | 291 |
| MS.gene88887.t1 | AT1G02050 | 39.894 | 376 | 216 | 5 | 15 | 387 | 24 | 392 | 7.11e-88 | 271 |
| MS.gene88887.t1 | AT4G34850 | 40.312 | 320 | 188 | 2 | 72 | 389 | 1 | 319 | 5.65e-82 | 254 |
| MS.gene88887.t1 | AT3G52160 | 26.337 | 243 | 159 | 8 | 150 | 387 | 97 | 324 | 6.02e-12 | 66.6 |
| MS.gene88887.t1 | AT3G52160 | 26.337 | 243 | 159 | 8 | 150 | 387 | 210 | 437 | 1.27e-11 | 66.2 |
Find 88 sgRNAs with CRISPR-Local
Find 118 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAATAGGTTTCTCAATTTC+TGG | 0.101255 | 1.1:-70441809 | None:intergenic |
| TGCAGTTCCAATGGCTAAAA+TGG | 0.225262 | 1.1:-70441005 | None:intergenic |
| CTGATTACAATTCAATCTTT+TGG | 0.254027 | 1.1:+70441999 | MS.gene88887:CDS |
| TATCAACAAGGTTGCTTTGC+AGG | 0.259527 | 1.1:+70441599 | MS.gene88887:CDS |
| CCTTTGAGCATTGCGAATTT+CGG | 0.276950 | 1.1:-70440966 | None:intergenic |
| AGAGAAGTGCTTAGTGAATA+TGG | 0.295286 | 1.1:+70442103 | MS.gene88887:CDS |
| CTTTCAAAATCTCCTCTGTT+AGG | 0.305425 | 1.1:-70441334 | None:intergenic |
| CTTGTTGGACAAGCACTATT+TGG | 0.314421 | 1.1:+70441746 | MS.gene88887:CDS |
| GCTTCGTTTGGCTAAAGATT+TGG | 0.342769 | 1.1:+70441631 | MS.gene88887:CDS |
| TCTACTTGATCTAGAATTGC+AGG | 0.346751 | 1.1:-70442040 | None:intergenic |
| TGAATACATGGCACCTTCAT+TGG | 0.347646 | 1.1:+70441373 | MS.gene88887:CDS |
| TCAATGGCTCCTTCACTATC+TGG | 0.348362 | 1.1:-70441863 | None:intergenic |
| GTTGTAGAGGTACCTAGATT+AGG | 0.360448 | 1.1:+70441416 | MS.gene88887:CDS |
| GGTTTCTCAATTTCTGGTAC+TGG | 0.362281 | 1.1:-70441803 | None:intergenic |
| AAGTGAGTAATCTTAGACTT+TGG | 0.381814 | 1.1:-70441479 | None:intergenic |
| AGGAGGCACGGTGCTTCGTT+TGG | 0.381968 | 1.1:+70441619 | MS.gene88887:CDS |
| TACCAACTCACAAAACTCTT+AGG | 0.382277 | 1.1:+70441545 | MS.gene88887:CDS |
| CCAATGGCTAAAATGGTTGC+AGG | 0.393820 | 1.1:-70440998 | None:intergenic |
| TTGTAGAGGTACCTAGATTA+GGG | 0.409559 | 1.1:+70441417 | MS.gene88887:CDS |
| GGAGCTGCTGCACTGATTGT+TGG | 0.411529 | 1.1:+70441773 | MS.gene88887:CDS |
| CACCTTCTAAAAGATGTTCC+CGG | 0.414732 | 1.1:+70441917 | MS.gene88887:CDS |
| AGTAAAAGCTATAAAAGAAT+GGG | 0.422344 | 1.1:+70441451 | MS.gene88887:CDS |
| ACTCACTTGGACAGCCTTGT+TGG | 0.426712 | 1.1:+70441731 | MS.gene88887:CDS |
| GTCACTGCAGTTACATTTCG+CGG | 0.450823 | 1.1:+70441698 | MS.gene88887:CDS |
| CAGTAAAAGCTATAAAAGAA+TGG | 0.459818 | 1.1:+70441450 | MS.gene88887:CDS |
| ATATTCACTAAGCACTTCTC+TGG | 0.467287 | 1.1:-70442101 | None:intergenic |
| CCGAAATTCGCAATGCTCAA+AGG | 0.469903 | 1.1:+70440966 | MS.gene88887:CDS |
| CGAAATTCGCAATGCTCAAA+GGG | 0.470864 | 1.1:+70440967 | MS.gene88887:CDS |
| TTCACGAAGGTGACCATCAA+TGG | 0.471758 | 1.1:-70441879 | None:intergenic |
| GTGAGTTGGTAATCAGCTCC+AGG | 0.479782 | 1.1:-70441533 | None:intergenic |
| GATAGTGAAGGAGCCATTGA+TGG | 0.482535 | 1.1:+70441866 | MS.gene88887:CDS |
| CTGTCCAAGTGAGTATCACT+AGG | 0.483602 | 1.1:-70441722 | None:intergenic |
| AAAGAACATCAATAAAGCAT+TGG | 0.485622 | 1.1:+70441949 | MS.gene88887:CDS |
| GATGGTCACCTTCGTGAAGC+TGG | 0.486168 | 1.1:+70441884 | MS.gene88887:CDS |
| CTTTGTTCAACACAGTTGGT+TGG | 0.489948 | 1.1:-70441031 | None:intergenic |
| TCTAGAATTGCAGGTCCACC+AGG | 0.500155 | 1.1:-70442031 | None:intergenic |
| TTGCGCAGTGTCGCTATATG+AGG | 0.502378 | 1.1:+70442271 | MS.gene88887:CDS |
| GTCTTCGCCCATATGTGAAA+AGG | 0.504788 | 1.1:+70441567 | MS.gene88887:CDS |
| GCAGCCTCCTTCCCTAATCT+AGG | 0.505127 | 1.1:-70441428 | None:intergenic |
| TGGCACCTTCATTGGATGCT+AGG | 0.506096 | 1.1:+70441381 | MS.gene88887:CDS |
| GATGTTCTTTGAAACAATCC+CGG | 0.506109 | 1.1:-70441936 | None:intergenic |
| GCGCAAAACAACAGTTTCAA+TGG | 0.506548 | 1.1:-70442254 | None:intergenic |
| ACCTTCTAAAAGATGTTCCC+GGG | 0.518985 | 1.1:+70441918 | MS.gene88887:CDS |
| GGACTGAAGACCACGGGAGA+AGG | 0.524843 | 1.1:+70442190 | MS.gene88887:CDS |
| GGTTGGATTTGCAGTTCCAA+TGG | 0.525311 | 1.1:-70441014 | None:intergenic |
| TCCTAGTGTTTGTGAATACA+TGG | 0.528394 | 1.1:+70441361 | MS.gene88887:CDS |
| CGGCCCTAGTGATACTCACT+TGG | 0.529910 | 1.1:+70441718 | MS.gene88887:CDS |
| CATCAATAAAGCATTGGTTG+AGG | 0.540608 | 1.1:+70441955 | MS.gene88887:CDS |
| ATGTTCTTTGAAACAATCCC+GGG | 0.547822 | 1.1:-70441935 | None:intergenic |
| CGCAATGCTCAAAGGGCAGA+AGG | 0.548900 | 1.1:+70440974 | MS.gene88887:CDS |
| AGGTTGCTTTGCAGGAGGCA+CGG | 0.555669 | 1.1:+70441607 | MS.gene88887:CDS |
| GGACAAGCACTATTTGGAGA+TGG | 0.563405 | 1.1:+70441752 | MS.gene88887:CDS |
| TTCACTAAGCACTTCTCTGG+TGG | 0.567752 | 1.1:-70442098 | None:intergenic |
| AGGCATGTCTACACCACTTG+TGG | 0.569916 | 1.1:-70441513 | None:intergenic |
| GCAAGACATGGTGGTTGTAG+AGG | 0.570536 | 1.1:+70441403 | MS.gene88887:CDS |
| ACAAGTGGTGTAGACATGCC+TGG | 0.572074 | 1.1:+70441515 | MS.gene88887:CDS |
| CACGGGAGAAGGACTTGAGT+GGG | 0.572386 | 1.1:+70442201 | MS.gene88887:CDS |
| CAAATAGTGCTTGTCCAACA+AGG | 0.572762 | 1.1:-70441745 | None:intergenic |
| TCTTGCCTAGCATCCAATGA+AGG | 0.579296 | 1.1:-70441386 | None:intergenic |
| GTGATAAATCTATGATCAAG+AGG | 0.584564 | 1.1:+70441300 | MS.gene88887:CDS |
| AGAGGTACCTAGATTAGGGA+AGG | 0.585699 | 1.1:+70441421 | MS.gene88887:CDS |
| TTGATCGTTTGCACCACAAG+TGG | 0.595788 | 1.1:+70441500 | MS.gene88887:CDS |
| AATGAGAAAGAAGTCCGCTC+AGG | 0.596562 | 1.1:+70442165 | MS.gene88887:CDS |
| AGAAAGAAGTCCGCTCAGGA+TGG | 0.597434 | 1.1:+70442169 | MS.gene88887:CDS |
| CAGGATGGACTGAAGACCAC+GGG | 0.604373 | 1.1:+70442184 | MS.gene88887:CDS |
| TGTGCTTTGTTCAACACAGT+TGG | 0.606666 | 1.1:-70441035 | None:intergenic |
| CCACTCAAGTCCTTCTCCCG+TGG | 0.610390 | 1.1:-70442200 | None:intergenic |
| GGTACCTAGATTAGGGAAGG+AGG | 0.615819 | 1.1:+70441424 | MS.gene88887:CDS |
| TTGTAATCAGAAATTCCCAA+TGG | 0.619803 | 1.1:-70441986 | None:intergenic |
| CAACAAAGGTGCTCGTGTGT+TGG | 0.620198 | 1.1:+70441661 | MS.gene88887:CDS |
| CAACAAGGTTGCTTTGCAGG+AGG | 0.628329 | 1.1:+70441602 | MS.gene88887:CDS |
| GAGATATATGTACCTAACAG+AGG | 0.637031 | 1.1:+70441322 | MS.gene88887:CDS |
| CCACGGGAGAAGGACTTGAG+TGG | 0.645541 | 1.1:+70442200 | MS.gene88887:CDS |
| ATTGGATGCTAGGCAAGACA+TGG | 0.648366 | 1.1:+70441391 | MS.gene88887:CDS |
| CAATGGCTAAAATGGTTGCA+GGG | 0.655281 | 1.1:-70440997 | None:intergenic |
| ACAATTGCTCCAGATAGTGA+AGG | 0.659707 | 1.1:+70441854 | MS.gene88887:CDS |
| GTAAAAGCTATAAAAGAATG+GGG | 0.663083 | 1.1:+70441452 | MS.gene88887:CDS |
| TGTCCAAGTGAGTATCACTA+GGG | 0.674277 | 1.1:-70441721 | None:intergenic |
| GGTCTTCAGTCCATCCTGAG+CGG | 0.678236 | 1.1:-70442179 | None:intergenic |
| ACAGTTTCAATGGTAAGACC+CGG | 0.678772 | 1.1:-70442244 | None:intergenic |
| TCAGGATGGACTGAAGACCA+CGG | 0.679433 | 1.1:+70442183 | MS.gene88887:CDS |
| GCCATGTATTCACAAACACT+AGG | 0.680230 | 1.1:-70441362 | None:intergenic |
| GATTTGGCTGAGAACAACAA+AGG | 0.683242 | 1.1:+70441647 | MS.gene88887:CDS |
| ATGTTAGTCCAGCTTCACGA+AGG | 0.698828 | 1.1:-70441892 | None:intergenic |
| CTAGAATTGCAGGTCCACCA+GGG | 0.711261 | 1.1:-70442030 | None:intergenic |
| AGGTATATGATGTATCAACA+AGG | 0.714291 | 1.1:+70441587 | MS.gene88887:CDS |
| ACGGGAGAAGGACTTGAGTG+GGG | 0.725736 | 1.1:+70442202 | MS.gene88887:CDS |
| GGATGCTAGGCAAGACATGG+TGG | 0.737305 | 1.1:+70441394 | MS.gene88887:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTATCTTTACATATGTAAA+TGG | + | chr1.1:70441191-70441210 | MS.gene88887:intron | 15.0% |
| !! | AATATTAGTTCAACAAAACT+TGG | - | chr1.1:70441258-70441277 | None:intergenic | 20.0% |
| !! | AGTAAAAGCTATAAAAGAAT+GGG | + | chr1.1:70441451-70441470 | MS.gene88887:CDS | 20.0% |
| !! | ATATTAGTTCAACAAAACTT+GGG | - | chr1.1:70441257-70441276 | None:intergenic | 20.0% |
| !! | TTATTACACTATCTTTACAT+TGG | + | chr1.1:70441221-70441240 | MS.gene88887:intron | 20.0% |
| ! | AAAATAGGTTTCTCAATTTC+TGG | - | chr1.1:70441812-70441831 | None:intergenic | 25.0% |
| ! | AAAGAACATCAATAAAGCAT+TGG | + | chr1.1:70441949-70441968 | MS.gene88887:CDS | 25.0% |
| ! | ATCTAAAAAAGTTAGGTAGA+GGG | - | chr1.1:70441149-70441168 | None:intergenic | 25.0% |
| ! | CAGTAAAAGCTATAAAAGAA+TGG | + | chr1.1:70441450-70441469 | MS.gene88887:CDS | 25.0% |
| ! | GTAAAAGCTATAAAAGAATG+GGG | + | chr1.1:70441452-70441471 | MS.gene88887:CDS | 25.0% |
| ! | TATCTAAAAAAGTTAGGTAG+AGG | - | chr1.1:70441150-70441169 | None:intergenic | 25.0% |
| !! | ATCATATACCTTTTCACATA+TGG | - | chr1.1:70441578-70441597 | None:intergenic | 25.0% |
| !! | CTGATTACAATTCAATCTTT+TGG | + | chr1.1:70441999-70442018 | MS.gene88887:CDS | 25.0% |
| !! | TCATATACCTTTTCACATAT+GGG | - | chr1.1:70441577-70441596 | None:intergenic | 25.0% |
| !!! | GGAAAGTATCTAAAAAAGTT+AGG | - | chr1.1:70441156-70441175 | None:intergenic | 25.0% |
| !!! | GTGATTTTAAAGTAGAAATC+AGG | - | chr1.1:70441064-70441083 | None:intergenic | 25.0% |
| AAAAATACTTACACATGCGT+TGG | - | chr1.1:70441123-70441142 | None:intergenic | 30.0% | |
| AAGTGAGTAATCTTAGACTT+TGG | - | chr1.1:70441482-70441501 | None:intergenic | 30.0% | |
| AGGTATATGATGTATCAACA+AGG | + | chr1.1:70441587-70441606 | MS.gene88887:CDS | 30.0% | |
| ATCATAGATTTATCACCTGA+AGG | - | chr1.1:70441296-70441315 | None:intergenic | 30.0% | |
| ATCTCTTCATAATTACCTTC+AGG | + | chr1.1:70441278-70441297 | MS.gene88887:intron | 30.0% | |
| GTGATAAATCTATGATCAAG+AGG | + | chr1.1:70441300-70441319 | MS.gene88887:CDS | 30.0% | |
| TTGTAATCAGAAATTCCCAA+TGG | - | chr1.1:70441989-70442008 | None:intergenic | 30.0% | |
| !! | TTTCATCTTTTCAGGCTTTA+AGG | - | chr1.1:70442077-70442096 | None:intergenic | 30.0% |
| !!! | AACCTATTTTTGAGATGGTT+TGG | + | chr1.1:70441822-70441841 | MS.gene88887:CDS | 30.0% |
| !!! | TGAGAAACCTATTTTTGAGA+TGG | + | chr1.1:70441817-70441836 | MS.gene88887:CDS | 30.0% |
| ATATTCACTAAGCACTTCTC+TGG | - | chr1.1:70442104-70442123 | None:intergenic | 35.0% | |
| CTTTCAAAATCTCCTCTGTT+AGG | - | chr1.1:70441337-70441356 | None:intergenic | 35.0% | |
| GAGATATATGTACCTAACAG+AGG | + | chr1.1:70441322-70441341 | MS.gene88887:CDS | 35.0% | |
| GTCCAAACCATCTCAAAAAT+AGG | - | chr1.1:70441827-70441846 | None:intergenic | 35.0% | |
| TACCAACTCACAAAACTCTT+AGG | + | chr1.1:70441545-70441564 | MS.gene88887:CDS | 35.0% | |
| TCCTAGTGTTTGTGAATACA+TGG | + | chr1.1:70441361-70441380 | MS.gene88887:CDS | 35.0% | |
| ! | TCTACTTGATCTAGAATTGC+AGG | - | chr1.1:70442043-70442062 | None:intergenic | 35.0% |
| !! | AGAGAAGTGCTTAGTGAATA+TGG | + | chr1.1:70442103-70442122 | MS.gene88887:CDS | 35.0% |
| !! | ATGTTCTTTGAAACAATCCC+GGG | - | chr1.1:70441938-70441957 | None:intergenic | 35.0% |
| !! | CATCAATAAAGCATTGGTTG+AGG | + | chr1.1:70441955-70441974 | MS.gene88887:CDS | 35.0% |
| !! | GATGTTCTTTGAAACAATCC+CGG | - | chr1.1:70441939-70441958 | None:intergenic | 35.0% |
| !! | TTGTAGAGGTACCTAGATTA+GGG | + | chr1.1:70441417-70441436 | MS.gene88887:CDS | 35.0% |
| !!! | TGTTTTATTTGGGTTTGGAC+CGG | + | chr1.1:70442225-70442244 | MS.gene88887:CDS | 35.0% |
| !!! | TTGAGTGGGGTGTTTTATTT+GGG | + | chr1.1:70442215-70442234 | MS.gene88887:CDS | 35.0% |
| ACAATTGCTCCAGATAGTGA+AGG | + | chr1.1:70441854-70441873 | MS.gene88887:CDS | 40.0% | |
| ACAGTTTCAATGGTAAGACC+CGG | - | chr1.1:70442247-70442266 | None:intergenic | 40.0% | |
| CAAATAGTGCTTGTCCAACA+AGG | - | chr1.1:70441748-70441767 | None:intergenic | 40.0% | |
| CAATGGCTAAAATGGTTGCA+GGG | - | chr1.1:70441000-70441019 | None:intergenic | 40.0% | |
| CGAAATTCGCAATGCTCAAA+GGG | + | chr1.1:70440967-70440986 | MS.gene88887:CDS | 40.0% | |
| CTTGTTGGACAAGCACTATT+TGG | + | chr1.1:70441746-70441765 | MS.gene88887:CDS | 40.0% | |
| CTTTGTTCAACACAGTTGGT+TGG | - | chr1.1:70441034-70441053 | None:intergenic | 40.0% | |
| GATTTGGCTGAGAACAACAA+AGG | + | chr1.1:70441647-70441666 | MS.gene88887:CDS | 40.0% | |
| GCCATGTATTCACAAACACT+AGG | - | chr1.1:70441365-70441384 | None:intergenic | 40.0% | |
| GCGCAAAACAACAGTTTCAA+TGG | - | chr1.1:70442257-70442276 | None:intergenic | 40.0% | |
| GCTTCGTTTGGCTAAAGATT+TGG | + | chr1.1:70441631-70441650 | MS.gene88887:CDS | 40.0% | |
| TGAATACATGGCACCTTCAT+TGG | + | chr1.1:70441373-70441392 | MS.gene88887:CDS | 40.0% | |
| TGCAGTTCCAATGGCTAAAA+TGG | - | chr1.1:70441008-70441027 | None:intergenic | 40.0% | |
| TGTCCAAGTGAGTATCACTA+GGG | - | chr1.1:70441724-70441743 | None:intergenic | 40.0% | |
| ! | ACCTTCTAAAAGATGTTCCC+GGG | + | chr1.1:70441918-70441937 | MS.gene88887:CDS | 40.0% |
| ! | CACCTTCTAAAAGATGTTCC+CGG | + | chr1.1:70441917-70441936 | MS.gene88887:CDS | 40.0% |
| ! | CCTTTGAGCATTGCGAATTT+CGG | - | chr1.1:70440969-70440988 | None:intergenic | 40.0% |
| ! | GTTGAGGCTTTTGAACCATT+GGG | + | chr1.1:70441971-70441990 | MS.gene88887:CDS | 40.0% |
| ! | TATCAACAAGGTTGCTTTGC+AGG | + | chr1.1:70441599-70441618 | MS.gene88887:CDS | 40.0% |
| ! | TGTGCTTTGTTCAACACAGT+TGG | - | chr1.1:70441038-70441057 | None:intergenic | 40.0% |
| !! | CTTGAGTGGGGTGTTTTATT+TGG | + | chr1.1:70442214-70442233 | MS.gene88887:CDS | 40.0% |
| !! | GACCTAAGAGTTTTGTGAGT+TGG | - | chr1.1:70441550-70441569 | None:intergenic | 40.0% |
| !! | GGTTTCTCAATTTCTGGTAC+TGG | - | chr1.1:70441806-70441825 | None:intergenic | 40.0% |
| !! | GTTGTAGAGGTACCTAGATT+AGG | + | chr1.1:70441416-70441435 | MS.gene88887:CDS | 40.0% |
| !!! | GTTTTATTTGGGTTTGGACC+GGG | + | chr1.1:70442226-70442245 | MS.gene88887:CDS | 40.0% |
| !!! | TGGGGTGTTTTATTTGGGTT+TGG | + | chr1.1:70442220-70442239 | MS.gene88887:CDS | 40.0% |
| AATGAGAAAGAAGTCCGCTC+AGG | + | chr1.1:70442165-70442184 | MS.gene88887:CDS | 45.0% | |
| ATGTTAGTCCAGCTTCACGA+AGG | - | chr1.1:70441895-70441914 | None:intergenic | 45.0% | |
| ATTGGATGCTAGGCAAGACA+TGG | + | chr1.1:70441391-70441410 | MS.gene88887:CDS | 45.0% | |
| CCAATGGCTAAAATGGTTGC+AGG | - | chr1.1:70441001-70441020 | None:intergenic | 45.0% | |
| CCGAAATTCGCAATGCTCAA+AGG | + | chr1.1:70440966-70440985 | MS.gene88887:CDS | 45.0% | |
| CTGTCCAAGTGAGTATCACT+AGG | - | chr1.1:70441725-70441744 | None:intergenic | 45.0% | |
| GATAGTGAAGGAGCCATTGA+TGG | + | chr1.1:70441866-70441885 | MS.gene88887:CDS | 45.0% | |
| GGACAAGCACTATTTGGAGA+TGG | + | chr1.1:70441752-70441771 | MS.gene88887:CDS | 45.0% | |
| GGTTGGATTTGCAGTTCCAA+TGG | - | chr1.1:70441017-70441036 | None:intergenic | 45.0% | |
| GTCACTGCAGTTACATTTCG+CGG | + | chr1.1:70441698-70441717 | MS.gene88887:CDS | 45.0% | |
| GTCTTCGCCCATATGTGAAA+AGG | + | chr1.1:70441567-70441586 | MS.gene88887:CDS | 45.0% | |
| TCAATGGCTCCTTCACTATC+TGG | - | chr1.1:70441866-70441885 | None:intergenic | 45.0% | |
| TCTTGCCTAGCATCCAATGA+AGG | - | chr1.1:70441389-70441408 | None:intergenic | 45.0% | |
| TTCACGAAGGTGACCATCAA+TGG | - | chr1.1:70441882-70441901 | None:intergenic | 45.0% | |
| TTCACTAAGCACTTCTCTGG+TGG | - | chr1.1:70442101-70442120 | None:intergenic | 45.0% | |
| TTGATCGTTTGCACCACAAG+TGG | + | chr1.1:70441500-70441519 | MS.gene88887:CDS | 45.0% | |
| ! | ATCTTTTGGATTGCACACCC+TGG | + | chr1.1:70442013-70442032 | MS.gene88887:CDS | 45.0% |
| ! | CTGGTGGCTTTCATCTTTTC+AGG | - | chr1.1:70442085-70442104 | None:intergenic | 45.0% |
| ! | GGTTGAGGCTTTTGAACCAT+TGG | + | chr1.1:70441970-70441989 | MS.gene88887:CDS | 45.0% |
| ! | TCCCGGGAACATCTTTTAGA+AGG | - | chr1.1:70441922-70441941 | None:intergenic | 45.0% |
| !! | AGAGGTACCTAGATTAGGGA+AGG | + | chr1.1:70441421-70441440 | MS.gene88887:CDS | 45.0% |
| !! | CCTGCAACCATTTTAGCCAT+TGG | + | chr1.1:70440998-70441017 | MS.gene88887:CDS | 45.0% |
| !! | GATAATAAAATATATTTAAA+TGG | - | chr1.1:70441177-70441196 | None:intergenic | 5.0% |
| ACAAGTGGTGTAGACATGCC+TGG | + | chr1.1:70441515-70441534 | MS.gene88887:CDS | 50.0% | |
| AGAAAGAAGTCCGCTCAGGA+TGG | + | chr1.1:70442169-70442188 | MS.gene88887:CDS | 50.0% | |
| AGGCATGTCTACACCACTTG+TGG | - | chr1.1:70441516-70441535 | None:intergenic | 50.0% | |
| CTAGAATTGCAGGTCCACCA+GGG | - | chr1.1:70442033-70442052 | None:intergenic | 50.0% | |
| GCAAGACATGGTGGTTGTAG+AGG | + | chr1.1:70441403-70441422 | MS.gene88887:CDS | 50.0% | |
| GGTACCTAGATTAGGGAAGG+AGG | + | chr1.1:70441424-70441443 | MS.gene88887:CDS | 50.0% | |
| TGGCACCTTCATTGGATGCT+AGG | + | chr1.1:70441381-70441400 | MS.gene88887:CDS | 50.0% | |
| ! | ACTCACTTGGACAGCCTTGT+TGG | + | chr1.1:70441731-70441750 | MS.gene88887:CDS | 50.0% |
| ! | CAACAAGGTTGCTTTGCAGG+AGG | + | chr1.1:70441602-70441621 | MS.gene88887:CDS | 50.0% |
| ! | TCTAGAATTGCAGGTCCACC+AGG | - | chr1.1:70442034-70442053 | None:intergenic | 50.0% |
| ! | TTTTGGATTGCACACCCTGG+TGG | + | chr1.1:70442016-70442035 | MS.gene88887:CDS | 50.0% |
| !! | CAACAAAGGTGCTCGTGTGT+TGG | + | chr1.1:70441661-70441680 | MS.gene88887:CDS | 50.0% |
| !! | GTGAGTTGGTAATCAGCTCC+AGG | - | chr1.1:70441536-70441555 | None:intergenic | 50.0% |
| !! | TCAGGATGGACTGAAGACCA+CGG | + | chr1.1:70442183-70442202 | MS.gene88887:CDS | 50.0% |
| ACGGGAGAAGGACTTGAGTG+GGG | + | chr1.1:70442202-70442221 | MS.gene88887:CDS | 55.0% | |
| CACGGGAGAAGGACTTGAGT+GGG | + | chr1.1:70442201-70442220 | MS.gene88887:CDS | 55.0% | |
| CGCAATGCTCAAAGGGCAGA+AGG | + | chr1.1:70440974-70440993 | MS.gene88887:CDS | 55.0% | |
| CGGCCCTAGTGATACTCACT+TGG | + | chr1.1:70441718-70441737 | MS.gene88887:CDS | 55.0% | |
| GATGGTCACCTTCGTGAAGC+TGG | + | chr1.1:70441884-70441903 | MS.gene88887:CDS | 55.0% | |
| GCAGCCTCCTTCCCTAATCT+AGG | - | chr1.1:70441431-70441450 | None:intergenic | 55.0% | |
| GGATGCTAGGCAAGACATGG+TGG | + | chr1.1:70441394-70441413 | MS.gene88887:CDS | 55.0% | |
| GGTCTTCAGTCCATCCTGAG+CGG | - | chr1.1:70442182-70442201 | None:intergenic | 55.0% | |
| ! | AGGTTGCTTTGCAGGAGGCA+CGG | + | chr1.1:70441607-70441626 | MS.gene88887:CDS | 55.0% |
| !! | CAGGATGGACTGAAGACCAC+GGG | + | chr1.1:70442184-70442203 | MS.gene88887:CDS | 55.0% |
| !! | GGAGCTGCTGCACTGATTGT+TGG | + | chr1.1:70441773-70441792 | MS.gene88887:CDS | 55.0% |
| CCACGGGAGAAGGACTTGAG+TGG | + | chr1.1:70442200-70442219 | MS.gene88887:CDS | 60.0% | |
| CCACTCAAGTCCTTCTCCCG+TGG | - | chr1.1:70442203-70442222 | None:intergenic | 60.0% | |
| GGACTGAAGACCACGGGAGA+AGG | + | chr1.1:70442190-70442209 | MS.gene88887:CDS | 60.0% | |
| ! | AGGAGGCACGGTGCTTCGTT+TGG | + | chr1.1:70441619-70441638 | MS.gene88887:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 70440953 | 70442291 | 70440953 | ID=MS.gene88887 |
| chr1.1 | mRNA | 70440953 | 70442291 | 70440953 | ID=MS.gene88887.t1;Parent=MS.gene88887 |
| chr1.1 | exon | 70440953 | 70441130 | 70440953 | ID=MS.gene88887.t1.exon1;Parent=MS.gene88887.t1 |
| chr1.1 | CDS | 70440953 | 70441130 | 70440953 | ID=cds.MS.gene88887.t1;Parent=MS.gene88887.t1 |
| chr1.1 | exon | 70441300 | 70442291 | 70441300 | ID=MS.gene88887.t1.exon2;Parent=MS.gene88887.t1 |
| chr1.1 | CDS | 70441300 | 70442291 | 70441300 | ID=cds.MS.gene88887.t1;Parent=MS.gene88887.t1 |
| Gene Sequence |
| Protein sequence |
