Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88888.t1 | XP_003592044.1 | 99.5 | 389 | 2 | 0 | 1 | 389 | 1 | 389 | 1.50E-221 | 778.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88888.t1 | P30075 | 98.2 | 389 | 7 | 0 | 1 | 389 | 1 | 389 | 9.7e-222 | 770.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88888.t1 | G7IAK9 | 99.5 | 389 | 2 | 0 | 1 | 389 | 1 | 389 | 1.1e-221 | 778.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049181 | MS.gene88888 | 0.81482 | 1.27E-51 | -1.69E-46 |
| MS.gene049232 | MS.gene88888 | -0.800071 | 1.71E-48 | -1.69E-46 |
| MS.gene049259 | MS.gene88888 | 0.81418 | 1.76E-51 | -1.69E-46 |
| MS.gene049281 | MS.gene88888 | 0.808629 | 2.82E-50 | -1.69E-46 |
| MS.gene049312 | MS.gene88888 | 0.803398 | 3.55E-49 | -1.69E-46 |
| MS.gene049320 | MS.gene88888 | -0.810586 | 1.07E-50 | -1.69E-46 |
| MS.gene04986 | MS.gene88888 | 0.805071 | 1.59E-49 | -1.69E-46 |
| MS.gene05015 | MS.gene88888 | 0.804467 | 2.13E-49 | -1.69E-46 |
| MS.gene050216 | MS.gene88888 | -0.805031 | 1.62E-49 | -1.69E-46 |
| MS.gene050363 | MS.gene88888 | 0.802757 | 4.82E-49 | -1.69E-46 |
| MS.gene050381 | MS.gene88888 | 0.868069 | 8.58E-66 | -1.69E-46 |
| MS.gene050504 | MS.gene88888 | -0.822214 | 2.67E-53 | -1.69E-46 |
| MS.gene050612 | MS.gene88888 | -0.819715 | 1.00E-52 | -1.69E-46 |
| MS.gene050868 | MS.gene88888 | -0.818242 | 2.17E-52 | -1.69E-46 |
| MS.gene050871 | MS.gene88888 | -0.817963 | 2.51E-52 | -1.69E-46 |
| MS.gene050883 | MS.gene88888 | 0.941988 | 1.59E-101 | -1.69E-46 |
| MS.gene051044 | MS.gene88888 | 0.845012 | 5.29E-59 | -1.69E-46 |
| MS.gene051127 | MS.gene88888 | 0.862867 | 3.74E-64 | -1.69E-46 |
| MS.gene051138 | MS.gene88888 | 0.813496 | 2.49E-51 | -1.69E-46 |
| MS.gene051364 | MS.gene88888 | 0.823183 | 1.59E-53 | -1.69E-46 |
| MS.gene051366 | MS.gene88888 | 0.810881 | 9.25E-51 | -1.69E-46 |
| MS.gene051410 | MS.gene88888 | 0.80034 | 1.51E-48 | -1.69E-46 |
| MS.gene05141 | MS.gene88888 | 0.830087 | 3.58E-55 | -1.69E-46 |
| MS.gene051946 | MS.gene88888 | 0.857856 | 1.23E-62 | -1.69E-46 |
| MS.gene052053 | MS.gene88888 | 0.819315 | 1.24E-52 | -1.69E-46 |
| MS.gene052089 | MS.gene88888 | 0.800283 | 1.55E-48 | -1.69E-46 |
| MS.gene052482 | MS.gene88888 | -0.810659 | 1.03E-50 | -1.69E-46 |
| MS.gene052486 | MS.gene88888 | -0.806346 | 8.60E-50 | -1.69E-46 |
| MS.gene053069 | MS.gene88888 | 0.829134 | 6.10E-55 | -1.69E-46 |
| MS.gene053420 | MS.gene88888 | 0.827757 | 1.31E-54 | -1.69E-46 |
| MS.gene053488 | MS.gene88888 | 0.815608 | 8.47E-52 | -1.69E-46 |
| MS.gene053549 | MS.gene88888 | 0.819452 | 1.15E-52 | -1.69E-46 |
| MS.gene053561 | MS.gene88888 | 0.838909 | 2.17E-57 | -1.69E-46 |
| MS.gene053826 | MS.gene88888 | 0.845932 | 2.98E-59 | -1.69E-46 |
| MS.gene054054 | MS.gene88888 | 0.81011 | 1.36E-50 | -1.69E-46 |
| MS.gene054056 | MS.gene88888 | 0.847894 | 8.66E-60 | -1.69E-46 |
| MS.gene054708 | MS.gene88888 | 0.831845 | 1.32E-55 | -1.69E-46 |
| MS.gene054954 | MS.gene88888 | -0.80051 | 1.39E-48 | -1.69E-46 |
| MS.gene055124 | MS.gene88888 | 0.868791 | 5.02E-66 | -1.69E-46 |
| MS.gene055139 | MS.gene88888 | 0.838589 | 2.63E-57 | -1.69E-46 |
| MS.gene055154 | MS.gene88888 | 0.867379 | 1.43E-65 | -1.69E-46 |
| MS.gene055202 | MS.gene88888 | 0.825615 | 4.26E-54 | -1.69E-46 |
| MS.gene055502 | MS.gene88888 | -0.815488 | 9.01E-52 | -1.69E-46 |
| MS.gene055549 | MS.gene88888 | 0.831884 | 1.30E-55 | -1.69E-46 |
| MS.gene055550 | MS.gene88888 | 0.832655 | 8.35E-56 | -1.69E-46 |
| MS.gene055551 | MS.gene88888 | 0.817255 | 3.63E-52 | -1.69E-46 |
| MS.gene05589 | MS.gene88888 | -0.80776 | 4.32E-50 | -1.69E-46 |
| MS.gene055942 | MS.gene88888 | 0.819124 | 1.37E-52 | -1.69E-46 |
| MS.gene055961 | MS.gene88888 | 0.811294 | 7.52E-51 | -1.69E-46 |
| MS.gene056224 | MS.gene88888 | 0.810503 | 1.12E-50 | -1.69E-46 |
| MS.gene056310 | MS.gene88888 | 0.89012 | 1.31E-73 | -1.69E-46 |
| MS.gene056311 | MS.gene88888 | 0.914973 | 1.02E-84 | -1.69E-46 |
| MS.gene056313 | MS.gene88888 | 0.863198 | 2.95E-64 | -1.69E-46 |
| MS.gene056504 | MS.gene88888 | -0.834369 | 3.12E-56 | -1.69E-46 |
| MS.gene056530 | MS.gene88888 | 0.838018 | 3.69E-57 | -1.69E-46 |
| MS.gene057014 | MS.gene88888 | 0.874413 | 6.89E-68 | -1.69E-46 |
| MS.gene058068 | MS.gene88888 | 0.803657 | 3.14E-49 | -1.69E-46 |
| MS.gene058093 | MS.gene88888 | 0.811595 | 6.47E-51 | -1.69E-46 |
| MS.gene058099 | MS.gene88888 | 0.843753 | 1.15E-58 | -1.69E-46 |
| MS.gene058108 | MS.gene88888 | 0.851708 | 7.44E-61 | -1.69E-46 |
| MS.gene058109 | MS.gene88888 | 0.90153 | 2.44E-78 | -1.69E-46 |
| MS.gene058111 | MS.gene88888 | 0.900847 | 4.86E-78 | -1.69E-46 |
| MS.gene058117 | MS.gene88888 | 0.813264 | 2.79E-51 | -1.69E-46 |
| MS.gene058171 | MS.gene88888 | 0.892025 | 2.32E-74 | -1.69E-46 |
| MS.gene058173 | MS.gene88888 | 0.893991 | 3.75E-75 | -1.69E-46 |
| MS.gene058175 | MS.gene88888 | 0.89597 | 5.78E-76 | -1.69E-46 |
| MS.gene058268 | MS.gene88888 | 0.825159 | 5.46E-54 | -1.69E-46 |
| MS.gene058269 | MS.gene88888 | 0.806725 | 7.16E-50 | -1.69E-46 |
| MS.gene058493 | MS.gene88888 | 0.870199 | 1.75E-66 | -1.69E-46 |
| MS.gene058494 | MS.gene88888 | 0.861073 | 1.33E-63 | -1.69E-46 |
| MS.gene058496 | MS.gene88888 | 0.825185 | 5.38E-54 | -1.69E-46 |
| MS.gene058628 | MS.gene88888 | 0.875392 | 3.19E-68 | -1.69E-46 |
| MS.gene058629 | MS.gene88888 | 0.863069 | 3.24E-64 | -1.69E-46 |
| MS.gene058630 | MS.gene88888 | 0.870355 | 1.55E-66 | -1.69E-46 |
| MS.gene05884 | MS.gene88888 | 0.805111 | 1.56E-49 | -1.69E-46 |
| MS.gene059022 | MS.gene88888 | -0.819585 | 1.08E-52 | -1.69E-46 |
| MS.gene059037 | MS.gene88888 | 0.800543 | 1.37E-48 | -1.69E-46 |
| MS.gene059045 | MS.gene88888 | 0.851169 | 1.06E-60 | -1.69E-46 |
| MS.gene059223 | MS.gene88888 | 0.816312 | 5.90E-52 | -1.69E-46 |
| MS.gene059690 | MS.gene88888 | 0.846549 | 2.03E-59 | -1.69E-46 |
| MS.gene059691 | MS.gene88888 | 0.819878 | 9.22E-53 | -1.69E-46 |
| MS.gene059694 | MS.gene88888 | 0.850703 | 1.43E-60 | -1.69E-46 |
| MS.gene059697 | MS.gene88888 | 0.852859 | 3.50E-61 | -1.69E-46 |
| MS.gene059865 | MS.gene88888 | -0.833143 | 6.32E-56 | -1.69E-46 |
| MS.gene060595 | MS.gene88888 | 0.82063 | 6.20E-53 | -1.69E-46 |
| MS.gene060778 | MS.gene88888 | -0.802583 | 5.24E-49 | -1.69E-46 |
| MS.gene06077 | MS.gene88888 | -0.828464 | 8.86E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88888.t1 | MTR_1g098140 | 99.486 | 389 | 2 | 0 | 1 | 389 | 1 | 389 | 0.0 | 803 |
| MS.gene88888.t1 | MTR_1g097935 | 99.229 | 389 | 3 | 0 | 1 | 389 | 1 | 389 | 0.0 | 801 |
| MS.gene88888.t1 | MTR_1g097910 | 98.972 | 389 | 4 | 0 | 1 | 389 | 1 | 389 | 0.0 | 801 |
| MS.gene88888.t1 | MTR_7g016800 | 98.715 | 389 | 5 | 0 | 1 | 389 | 1 | 389 | 0.0 | 798 |
| MS.gene88888.t1 | MTR_7g016820 | 97.943 | 389 | 8 | 0 | 1 | 389 | 1 | 389 | 0.0 | 792 |
| MS.gene88888.t1 | MTR_7g016700 | 97.943 | 389 | 8 | 0 | 1 | 389 | 1 | 389 | 0.0 | 791 |
| MS.gene88888.t1 | MTR_7g016720 | 98.201 | 389 | 7 | 0 | 1 | 389 | 1 | 389 | 0.0 | 791 |
| MS.gene88888.t1 | MTR_5g007730 | 97.429 | 389 | 10 | 0 | 1 | 389 | 1 | 389 | 0.0 | 791 |
| MS.gene88888.t1 | MTR_5g007760 | 97.686 | 389 | 9 | 0 | 1 | 389 | 1 | 389 | 0.0 | 790 |
| MS.gene88888.t1 | MTR_7g016780 | 97.429 | 389 | 10 | 0 | 1 | 389 | 1 | 389 | 0.0 | 788 |
| MS.gene88888.t1 | MTR_5g007713 | 96.658 | 389 | 13 | 0 | 1 | 389 | 1 | 389 | 0.0 | 783 |
| MS.gene88888.t1 | MTR_5g007717 | 96.401 | 389 | 14 | 0 | 1 | 389 | 1 | 389 | 0.0 | 782 |
| MS.gene88888.t1 | MTR_3g083920 | 90.979 | 388 | 35 | 0 | 1 | 388 | 1 | 388 | 0.0 | 751 |
| MS.gene88888.t1 | MTR_3g083910 | 90.181 | 387 | 38 | 0 | 1 | 387 | 1 | 387 | 0.0 | 746 |
| MS.gene88888.t1 | MTR_2g058470 | 87.404 | 389 | 49 | 0 | 1 | 389 | 1 | 389 | 0.0 | 716 |
| MS.gene88888.t1 | MTR_3g086260 | 81.912 | 387 | 70 | 0 | 1 | 387 | 1 | 387 | 0.0 | 679 |
| MS.gene88888.t1 | MTR_7g084300 | 80.463 | 389 | 76 | 0 | 1 | 389 | 1 | 389 | 0.0 | 674 |
| MS.gene88888.t1 | MTR_7g113405 | 95.710 | 303 | 13 | 0 | 87 | 389 | 1 | 303 | 0.0 | 598 |
| MS.gene88888.t1 | MTR_3g088675 | 66.324 | 389 | 131 | 0 | 1 | 389 | 1 | 389 | 0.0 | 577 |
| MS.gene88888.t1 | MTR_5g007740 | 80.061 | 326 | 65 | 0 | 64 | 389 | 1 | 326 | 0.0 | 549 |
| MS.gene88888.t1 | MTR_5g007723 | 69.018 | 397 | 74 | 5 | 12 | 389 | 32 | 398 | 0.0 | 534 |
| MS.gene88888.t1 | MTR_5g007770 | 77.667 | 300 | 34 | 2 | 70 | 369 | 1 | 267 | 1.77e-167 | 469 |
| MS.gene88888.t1 | MTR_3g088670 | 49.100 | 389 | 184 | 6 | 10 | 387 | 2 | 387 | 2.42e-130 | 380 |
| MS.gene88888.t1 | MTR_4g075560 | 42.632 | 380 | 211 | 4 | 11 | 387 | 13 | 388 | 1.51e-102 | 309 |
| MS.gene88888.t1 | MTR_4g078730 | 37.802 | 373 | 225 | 2 | 15 | 387 | 24 | 389 | 7.12e-94 | 286 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88888.t1 | AT5G13930 | 82.732 | 388 | 66 | 1 | 3 | 389 | 8 | 395 | 0.0 | 675 |
| MS.gene88888.t1 | AT4G34850 | 40.849 | 377 | 219 | 3 | 15 | 389 | 18 | 392 | 1.30e-97 | 296 |
| MS.gene88888.t1 | AT4G00040 | 40.584 | 377 | 214 | 5 | 14 | 387 | 16 | 385 | 1.30e-95 | 291 |
| MS.gene88888.t1 | AT1G02050 | 40.053 | 377 | 216 | 5 | 15 | 388 | 24 | 393 | 1.64e-88 | 273 |
| MS.gene88888.t1 | AT4G34850 | 40.312 | 320 | 188 | 2 | 72 | 389 | 1 | 319 | 4.31e-82 | 254 |
| MS.gene88888.t1 | AT3G52160 | 26.749 | 243 | 158 | 9 | 150 | 387 | 97 | 324 | 3.51e-12 | 67.4 |
| MS.gene88888.t1 | AT3G52160 | 26.749 | 243 | 158 | 8 | 150 | 387 | 210 | 437 | 8.67e-12 | 67.0 |
Find 92 sgRNAs with CRISPR-Local
Find 119 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAATAGGTTTCTCAATTTC+TGG | 0.101255 | 1.1:-70448977 | None:intergenic |
| TGCAGTTCCAATGGCTAAAA+TGG | 0.233615 | 1.1:-70448212 | None:intergenic |
| CTGATTACAATTCAATCTTT+TGG | 0.254027 | 1.1:+70449167 | MS.gene88888:CDS |
| TATCAACAAGGTTGCTTTGC+AGG | 0.259527 | 1.1:+70448767 | MS.gene88888:CDS |
| CCTTTGAGCATTGCGAATTT+CGG | 0.276950 | 1.1:-70448173 | None:intergenic |
| GGTTTCTCAATTTCTGGTAT+TGG | 0.282073 | 1.1:-70448971 | None:intergenic |
| AGAGAAGTGCTTAGTGAATA+TGG | 0.295286 | 1.1:+70449271 | MS.gene88888:CDS |
| CTTTCAAAATCTCCTCTGTT+AGG | 0.305425 | 1.1:-70448502 | None:intergenic |
| TCAATGGCCCCTTCACTATC+TGG | 0.309674 | 1.1:-70449031 | None:intergenic |
| CTTGTTGGACAAGCACTATT+TGG | 0.314421 | 1.1:+70448914 | MS.gene88888:CDS |
| GCTTCGTTTGGCTAAAGATT+TGG | 0.342769 | 1.1:+70448799 | MS.gene88888:CDS |
| TGAATACATGGCACCTTCAT+TGG | 0.347646 | 1.1:+70448541 | MS.gene88888:CDS |
| GTTGTAGAGGTACCTAGATT+AGG | 0.360448 | 1.1:+70448584 | MS.gene88888:CDS |
| TCTACTTGATCTAGAATTGC+AGG | 0.378004 | 1.1:-70449208 | None:intergenic |
| AAGTGAGTAATCTTTGACTT+TGG | 0.381814 | 1.1:-70448647 | None:intergenic |
| AGGAGGCACGGTGCTTCGTT+TGG | 0.381968 | 1.1:+70448787 | MS.gene88888:CDS |
| TACCAACTCACAAAACTCTT+AGG | 0.382277 | 1.1:+70448713 | MS.gene88888:CDS |
| CTTTGTTCAACACAATTGGC+TGG | 0.390254 | 1.1:-70448238 | None:intergenic |
| CCAATGGCTAAAATGGTTGC+AGG | 0.393820 | 1.1:-70448205 | None:intergenic |
| CACCTTCTTAAAGATGTTCC+CGG | 0.401953 | 1.1:+70449085 | MS.gene88888:CDS |
| TTGTAGAGGTACCTAGATTA+GGG | 0.409559 | 1.1:+70448585 | MS.gene88888:CDS |
| GGAGCTGCTGCACTGATTGT+TGG | 0.411529 | 1.1:+70448941 | MS.gene88888:CDS |
| AGTAAAAGCTATAAAAGAAT+GGG | 0.422344 | 1.1:+70448619 | MS.gene88888:CDS |
| ACTCACTTGGACAGCCTTGT+TGG | 0.426712 | 1.1:+70448899 | MS.gene88888:CDS |
| GATAGTGAAGGGGCCATTGA+TGG | 0.440309 | 1.1:+70449034 | MS.gene88888:CDS |
| GTCACTGCAGTTACATTTCG+CGG | 0.450823 | 1.1:+70448866 | MS.gene88888:CDS |
| TTGCGTAGTGTCACTATATG+AGG | 0.451569 | 1.1:+70449439 | MS.gene88888:CDS |
| GTGATAAATCTATGATCAAA+AGG | 0.453113 | 1.1:+70448468 | MS.gene88888:CDS |
| CAGTAAAAGCTATAAAAGAA+TGG | 0.459818 | 1.1:+70448618 | MS.gene88888:CDS |
| ACGCAAAACAACGGTTTCAA+TGG | 0.461439 | 1.1:-70449422 | None:intergenic |
| ATATTCACTAAGCACTTCTC+TGG | 0.467287 | 1.1:-70449269 | None:intergenic |
| CCGAAATTCGCAATGCTCAA+AGG | 0.469903 | 1.1:+70448173 | MS.gene88888:CDS |
| TTCACGAAGGTGACCATCAA+TGG | 0.470504 | 1.1:-70449047 | None:intergenic |
| CGAAATTCGCAATGCTCAAA+GGG | 0.470864 | 1.1:+70448174 | MS.gene88888:CDS |
| GTGAGTTGGTAATCAGCTCC+AGG | 0.479782 | 1.1:-70448701 | None:intergenic |
| CTGTCCAAGTGAGTATCACT+AGG | 0.483602 | 1.1:-70448890 | None:intergenic |
| AAAGAACATCAATAAAGCAT+TGG | 0.485622 | 1.1:+70449117 | MS.gene88888:CDS |
| GATGGTCACCTTCGTGAAGC+TGG | 0.486168 | 1.1:+70449052 | MS.gene88888:CDS |
| TGTGCTTTGTTCAACACAAT+TGG | 0.494990 | 1.1:-70448242 | None:intergenic |
| TCTAGAATTGCAGGTCCACC+AGG | 0.500155 | 1.1:-70449199 | None:intergenic |
| GGCTGGGTTTGCAGTTCCAA+TGG | 0.502395 | 1.1:-70448221 | None:intergenic |
| GTCTTCGCCCATATGTGAAA+AGG | 0.504788 | 1.1:+70448735 | MS.gene88888:CDS |
| GCAGCCTCCTTCCCTAATCT+AGG | 0.505127 | 1.1:-70448596 | None:intergenic |
| TGGCACCTTCATTGGATGCT+AGG | 0.506096 | 1.1:+70448549 | MS.gene88888:CDS |
| GATGTTCTTTGAAACAATCC+CGG | 0.506109 | 1.1:-70449104 | None:intergenic |
| GGACTGAAGACCACGGGAGA+AGG | 0.524843 | 1.1:+70449358 | MS.gene88888:CDS |
| TCCTAGTGTTTGTGAATACA+TGG | 0.528394 | 1.1:+70448529 | MS.gene88888:CDS |
| CGGCCCTAGTGATACTCACT+TGG | 0.529910 | 1.1:+70448886 | MS.gene88888:CDS |
| TTTGTTCAACACAATTGGCT+GGG | 0.538613 | 1.1:-70448237 | None:intergenic |
| CATCAATAAAGCATTGGTTG+AGG | 0.540608 | 1.1:+70449123 | MS.gene88888:CDS |
| ACCTTCTTAAAGATGTTCCC+GGG | 0.543150 | 1.1:+70449086 | MS.gene88888:CDS |
| ATGTTCTTTGAAACAATCCC+GGG | 0.547822 | 1.1:-70449103 | None:intergenic |
| CGCAATGCTCAAAGGGCAGA+AGG | 0.548900 | 1.1:+70448181 | MS.gene88888:CDS |
| AGAAAGAAGTCGGCTCAAGA+TGG | 0.555109 | 1.1:+70449337 | MS.gene88888:CDS |
| AGGTTGCTTTGCAGGAGGCA+CGG | 0.555669 | 1.1:+70448775 | MS.gene88888:CDS |
| GGACAAGCACTATTTGGAGA+TGG | 0.563405 | 1.1:+70448920 | MS.gene88888:CDS |
| TTCACTAAGCACTTCTCTGG+TGG | 0.567752 | 1.1:-70449266 | None:intergenic |
| TCCCGGGAACATCTTTAAGA+AGG | 0.569831 | 1.1:-70449087 | None:intergenic |
| AGGCATGTCTACACCACTTG+TGG | 0.569916 | 1.1:-70448681 | None:intergenic |
| GCAAGACATGGTGGTTGTAG+AGG | 0.570536 | 1.1:+70448571 | MS.gene88888:CDS |
| ACAAGTGGTGTAGACATGCC+TGG | 0.572074 | 1.1:+70448683 | MS.gene88888:CDS |
| CACGGGAGAAGGACTTGAGT+GGG | 0.572386 | 1.1:+70449369 | MS.gene88888:CDS |
| CAAATAGTGCTTGTCCAACA+AGG | 0.572762 | 1.1:-70448913 | None:intergenic |
| TCTTGCCTAGCATCCAATGA+AGG | 0.579296 | 1.1:-70448554 | None:intergenic |
| AGATGAAATGAGAAAGAAGT+CGG | 0.582002 | 1.1:+70449327 | MS.gene88888:CDS |
| AGAGGTACCTAGATTAGGGA+AGG | 0.585699 | 1.1:+70448589 | MS.gene88888:CDS |
| CAATTGCTCCAGATAGTGAA+GGG | 0.593639 | 1.1:+70449023 | MS.gene88888:CDS |
| TTGATCGTTTGCACCACAAG+TGG | 0.595788 | 1.1:+70448668 | MS.gene88888:CDS |
| CAAGATGGACTGAAGACCAC+GGG | 0.604373 | 1.1:+70449352 | MS.gene88888:CDS |
| ACAATTGCTCCAGATAGTGA+AGG | 0.608112 | 1.1:+70449022 | MS.gene88888:CDS |
| CCACTCAAGTCCTTCTCCCG+TGG | 0.610390 | 1.1:-70449368 | None:intergenic |
| ACGGTTTCAATGGTAAGCCC+CGG | 0.611403 | 1.1:-70449412 | None:intergenic |
| GGTACCTAGATTAGGGAAGG+AGG | 0.615819 | 1.1:+70448592 | MS.gene88888:CDS |
| TTGTAATCAGAAATTCCCAA+TGG | 0.619803 | 1.1:-70449154 | None:intergenic |
| CAACAAAGGTGCTCGTGTGT+TGG | 0.620198 | 1.1:+70448829 | MS.gene88888:CDS |
| CAACAAGGTTGCTTTGCAGG+AGG | 0.628329 | 1.1:+70448770 | MS.gene88888:CDS |
| CCACGGGAGAAGGACTTGAG+TGG | 0.645541 | 1.1:+70449368 | MS.gene88888:CDS |
| ATTGGATGCTAGGCAAGACA+TGG | 0.648366 | 1.1:+70448559 | MS.gene88888:CDS |
| CAATGGCTAAAATGGTTGCA+GGG | 0.655281 | 1.1:-70448204 | None:intergenic |
| GTAAAAGCTATAAAAGAATG+GGG | 0.663083 | 1.1:+70448620 | MS.gene88888:CDS |
| TCAAGATGGACTGAAGACCA+CGG | 0.668535 | 1.1:+70449351 | MS.gene88888:CDS |
| TGTCCAAGTGAGTATCACTA+GGG | 0.674277 | 1.1:-70448889 | None:intergenic |
| GCCATGTATTCACAAACACT+AGG | 0.680230 | 1.1:-70448530 | None:intergenic |
| GATTTGGCTGAGAACAACAA+AGG | 0.683242 | 1.1:+70448815 | MS.gene88888:CDS |
| AGTGACACTACGCAAAACAA+CGG | 0.695175 | 1.1:-70449431 | None:intergenic |
| ATGTTAGTCCAGCTTCACGA+AGG | 0.698828 | 1.1:-70449060 | None:intergenic |
| AATTGCTCCAGATAGTGAAG+GGG | 0.700404 | 1.1:+70449024 | MS.gene88888:CDS |
| CTAGAATTGCAGGTCCACCA+GGG | 0.711261 | 1.1:-70449198 | None:intergenic |
| GAGATACATGTACCTAACAG+AGG | 0.713175 | 1.1:+70448490 | MS.gene88888:CDS |
| AGGTATATGATGTATCAACA+AGG | 0.714291 | 1.1:+70448755 | MS.gene88888:CDS |
| ACGGGAGAAGGACTTGAGTG+GGG | 0.725736 | 1.1:+70449370 | MS.gene88888:CDS |
| GGATGCTAGGCAAGACATGG+TGG | 0.737305 | 1.1:+70448562 | MS.gene88888:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGTAAAAGCTATAAAAGAAT+GGG | + | chr1.1:70448619-70448638 | MS.gene88888:CDS | 20.0% |
| !! | GATAGCAAAATATGTTTAAA+TGG | - | chr1.1:70448383-70448402 | None:intergenic | 20.0% |
| !!! | ATATTTTGCTATCTTTACAT+TGG | + | chr1.1:70448390-70448409 | MS.gene88888:intron | 20.0% |
| ! | AAAATAGGTTTCTCAATTTC+TGG | - | chr1.1:70448980-70448999 | None:intergenic | 25.0% |
| ! | AAAGAACATCAATAAAGCAT+TGG | + | chr1.1:70449117-70449136 | MS.gene88888:CDS | 25.0% |
| ! | ATTTCTTCATAATTACCTTC+AGG | + | chr1.1:70448446-70448465 | MS.gene88888:intron | 25.0% |
| ! | CAGTAAAAGCTATAAAAGAA+TGG | + | chr1.1:70448618-70448637 | MS.gene88888:CDS | 25.0% |
| ! | GTAAAAGCTATAAAAGAATG+GGG | + | chr1.1:70448620-70448639 | MS.gene88888:CDS | 25.0% |
| ! | GTGATAAATCTATGATCAAA+AGG | + | chr1.1:70448468-70448487 | MS.gene88888:CDS | 25.0% |
| ! | TATCTAAAAAAGTTAGGTAG+AGG | - | chr1.1:70448356-70448375 | None:intergenic | 25.0% |
| !! | ATCATATACCTTTTCACATA+TGG | - | chr1.1:70448746-70448765 | None:intergenic | 25.0% |
| !! | CTGATTACAATTCAATCTTT+TGG | + | chr1.1:70449167-70449186 | MS.gene88888:CDS | 25.0% |
| !! | TCATATACCTTTTCACATAT+GGG | - | chr1.1:70448745-70448764 | None:intergenic | 25.0% |
| !!! | GGAAAGTATCTAAAAAAGTT+AGG | - | chr1.1:70448362-70448381 | None:intergenic | 25.0% |
| !!! | GTGATTTTAAAGTAGAAATC+AGG | - | chr1.1:70448271-70448290 | None:intergenic | 25.0% |
| !!! | TTTCATCTTTTCAGGTTTTA+AGG | - | chr1.1:70449245-70449264 | None:intergenic | 25.0% |
| AAAAATACTTACACATGCGT+TGG | - | chr1.1:70448330-70448349 | None:intergenic | 30.0% | |
| AGATGAAATGAGAAAGAAGT+CGG | + | chr1.1:70449327-70449346 | MS.gene88888:CDS | 30.0% | |
| AGGTATATGATGTATCAACA+AGG | + | chr1.1:70448755-70448774 | MS.gene88888:CDS | 30.0% | |
| ATCATAGATTTATCACCTGA+AGG | - | chr1.1:70448464-70448483 | None:intergenic | 30.0% | |
| GATATTAGTTCAGCAAACTT+GGG | - | chr1.1:70448426-70448445 | None:intergenic | 30.0% | |
| TGATATTAGTTCAGCAAACT+TGG | - | chr1.1:70448427-70448446 | None:intergenic | 30.0% | |
| TTGTAATCAGAAATTCCCAA+TGG | - | chr1.1:70449157-70449176 | None:intergenic | 30.0% | |
| ! | AAGTGAGTAATCTTTGACTT+TGG | - | chr1.1:70448650-70448669 | None:intergenic | 30.0% |
| !!! | AACCTATTTTTGAGATGGTT+TGG | + | chr1.1:70448990-70449009 | MS.gene88888:CDS | 30.0% |
| !!! | TGAGAAACCTATTTTTGAGA+TGG | + | chr1.1:70448985-70449004 | MS.gene88888:CDS | 30.0% |
| ATATTCACTAAGCACTTCTC+TGG | - | chr1.1:70449272-70449291 | None:intergenic | 35.0% | |
| CTTTCAAAATCTCCTCTGTT+AGG | - | chr1.1:70448505-70448524 | None:intergenic | 35.0% | |
| GTCCAAACCATCTCAAAAAT+AGG | - | chr1.1:70448995-70449014 | None:intergenic | 35.0% | |
| TACCAACTCACAAAACTCTT+AGG | + | chr1.1:70448713-70448732 | MS.gene88888:CDS | 35.0% | |
| TCCTAGTGTTTGTGAATACA+TGG | + | chr1.1:70448529-70448548 | MS.gene88888:CDS | 35.0% | |
| TTTGTTCAACACAATTGGCT+GGG | - | chr1.1:70448240-70448259 | None:intergenic | 35.0% | |
| ! | TCTACTTGATCTAGAATTGC+AGG | - | chr1.1:70449211-70449230 | None:intergenic | 35.0% |
| ! | TGTGCTTTGTTCAACACAAT+TGG | - | chr1.1:70448245-70448264 | None:intergenic | 35.0% |
| !! | AGAGAAGTGCTTAGTGAATA+TGG | + | chr1.1:70449271-70449290 | MS.gene88888:CDS | 35.0% |
| !! | ATGTTCTTTGAAACAATCCC+GGG | - | chr1.1:70449106-70449125 | None:intergenic | 35.0% |
| !! | CATCAATAAAGCATTGGTTG+AGG | + | chr1.1:70449123-70449142 | MS.gene88888:CDS | 35.0% |
| !! | GATGTTCTTTGAAACAATCC+CGG | - | chr1.1:70449107-70449126 | None:intergenic | 35.0% |
| !! | GGTTTCTCAATTTCTGGTAT+TGG | - | chr1.1:70448974-70448993 | None:intergenic | 35.0% |
| !! | TTGTAGAGGTACCTAGATTA+GGG | + | chr1.1:70448585-70448604 | MS.gene88888:CDS | 35.0% |
| !!! | TGTTTTATTTGGCTTTGGAC+CGG | + | chr1.1:70449393-70449412 | MS.gene88888:CDS | 35.0% |
| AATTGCTCCAGATAGTGAAG+GGG | + | chr1.1:70449024-70449043 | MS.gene88888:CDS | 40.0% | |
| ACAATTGCTCCAGATAGTGA+AGG | + | chr1.1:70449022-70449041 | MS.gene88888:CDS | 40.0% | |
| ACCTTCTTAAAGATGTTCCC+GGG | + | chr1.1:70449086-70449105 | MS.gene88888:CDS | 40.0% | |
| ACGCAAAACAACGGTTTCAA+TGG | - | chr1.1:70449425-70449444 | None:intergenic | 40.0% | |
| AGTGACACTACGCAAAACAA+CGG | - | chr1.1:70449434-70449453 | None:intergenic | 40.0% | |
| CAAATAGTGCTTGTCCAACA+AGG | - | chr1.1:70448916-70448935 | None:intergenic | 40.0% | |
| CAATGGCTAAAATGGTTGCA+GGG | - | chr1.1:70448207-70448226 | None:intergenic | 40.0% | |
| CAATTGCTCCAGATAGTGAA+GGG | + | chr1.1:70449023-70449042 | MS.gene88888:CDS | 40.0% | |
| CACCTTCTTAAAGATGTTCC+CGG | + | chr1.1:70449085-70449104 | MS.gene88888:CDS | 40.0% | |
| CGAAATTCGCAATGCTCAAA+GGG | + | chr1.1:70448174-70448193 | MS.gene88888:CDS | 40.0% | |
| CTTGTTGGACAAGCACTATT+TGG | + | chr1.1:70448914-70448933 | MS.gene88888:CDS | 40.0% | |
| CTTTGTTCAACACAATTGGC+TGG | - | chr1.1:70448241-70448260 | None:intergenic | 40.0% | |
| GAGATACATGTACCTAACAG+AGG | + | chr1.1:70448490-70448509 | MS.gene88888:CDS | 40.0% | |
| GATTTGGCTGAGAACAACAA+AGG | + | chr1.1:70448815-70448834 | MS.gene88888:CDS | 40.0% | |
| GCCATGTATTCACAAACACT+AGG | - | chr1.1:70448533-70448552 | None:intergenic | 40.0% | |
| GCTTCGTTTGGCTAAAGATT+TGG | + | chr1.1:70448799-70448818 | MS.gene88888:CDS | 40.0% | |
| TGAATACATGGCACCTTCAT+TGG | + | chr1.1:70448541-70448560 | MS.gene88888:CDS | 40.0% | |
| TGCAGTTCCAATGGCTAAAA+TGG | - | chr1.1:70448215-70448234 | None:intergenic | 40.0% | |
| TGTCCAAGTGAGTATCACTA+GGG | - | chr1.1:70448892-70448911 | None:intergenic | 40.0% | |
| ! | CCTTTGAGCATTGCGAATTT+CGG | - | chr1.1:70448176-70448195 | None:intergenic | 40.0% |
| ! | GTTGAGGCTTTTGAACCATT+GGG | + | chr1.1:70449139-70449158 | MS.gene88888:CDS | 40.0% |
| ! | TATCAACAAGGTTGCTTTGC+AGG | + | chr1.1:70448767-70448786 | MS.gene88888:CDS | 40.0% |
| !! | CTTGAGTGGGGTGTTTTATT+TGG | + | chr1.1:70449382-70449401 | MS.gene88888:CDS | 40.0% |
| !! | GACCTAAGAGTTTTGTGAGT+TGG | - | chr1.1:70448718-70448737 | None:intergenic | 40.0% |
| !! | GTTGTAGAGGTACCTAGATT+AGG | + | chr1.1:70448584-70448603 | MS.gene88888:CDS | 40.0% |
| !!! | GTTTTATTTGGCTTTGGACC+GGG | + | chr1.1:70449394-70449413 | MS.gene88888:CDS | 40.0% |
| !!! | TGGGGTGTTTTATTTGGCTT+TGG | + | chr1.1:70449388-70449407 | MS.gene88888:CDS | 40.0% |
| !!! | TTTTATTTGGCTTTGGACCG+GGG | + | chr1.1:70449395-70449414 | MS.gene88888:CDS | 40.0% |
| ATGTTAGTCCAGCTTCACGA+AGG | - | chr1.1:70449063-70449082 | None:intergenic | 45.0% | |
| ATTGGATGCTAGGCAAGACA+TGG | + | chr1.1:70448559-70448578 | MS.gene88888:CDS | 45.0% | |
| CCAATGGCTAAAATGGTTGC+AGG | - | chr1.1:70448208-70448227 | None:intergenic | 45.0% | |
| CCGAAATTCGCAATGCTCAA+AGG | + | chr1.1:70448173-70448192 | MS.gene88888:CDS | 45.0% | |
| CTGTCCAAGTGAGTATCACT+AGG | - | chr1.1:70448893-70448912 | None:intergenic | 45.0% | |
| GGACAAGCACTATTTGGAGA+TGG | + | chr1.1:70448920-70448939 | MS.gene88888:CDS | 45.0% | |
| GTCACTGCAGTTACATTTCG+CGG | + | chr1.1:70448866-70448885 | MS.gene88888:CDS | 45.0% | |
| GTCTTCGCCCATATGTGAAA+AGG | + | chr1.1:70448735-70448754 | MS.gene88888:CDS | 45.0% | |
| TCTTGCCTAGCATCCAATGA+AGG | - | chr1.1:70448557-70448576 | None:intergenic | 45.0% | |
| TTCACGAAGGTGACCATCAA+TGG | - | chr1.1:70449050-70449069 | None:intergenic | 45.0% | |
| TTCACTAAGCACTTCTCTGG+TGG | - | chr1.1:70449269-70449288 | None:intergenic | 45.0% | |
| TTGATCGTTTGCACCACAAG+TGG | + | chr1.1:70448668-70448687 | MS.gene88888:CDS | 45.0% | |
| ! | AGAAAGAAGTCGGCTCAAGA+TGG | + | chr1.1:70449337-70449356 | MS.gene88888:CDS | 45.0% |
| ! | ATCTTTTGGATTGCACACCC+TGG | + | chr1.1:70449181-70449200 | MS.gene88888:CDS | 45.0% |
| ! | CTGGTGGCTTTCATCTTTTC+AGG | - | chr1.1:70449253-70449272 | None:intergenic | 45.0% |
| ! | GGTTGAGGCTTTTGAACCAT+TGG | + | chr1.1:70449138-70449157 | MS.gene88888:CDS | 45.0% |
| ! | TCCCGGGAACATCTTTAAGA+AGG | - | chr1.1:70449090-70449109 | None:intergenic | 45.0% |
| !! | AGAGGTACCTAGATTAGGGA+AGG | + | chr1.1:70448589-70448608 | MS.gene88888:CDS | 45.0% |
| !! | CCTGCAACCATTTTAGCCAT+TGG | + | chr1.1:70448205-70448224 | MS.gene88888:CDS | 45.0% |
| !! | TCAAGATGGACTGAAGACCA+CGG | + | chr1.1:70449351-70449370 | MS.gene88888:CDS | 45.0% |
| ACAAGTGGTGTAGACATGCC+TGG | + | chr1.1:70448683-70448702 | MS.gene88888:CDS | 50.0% | |
| ACGGTTTCAATGGTAAGCCC+CGG | - | chr1.1:70449415-70449434 | None:intergenic | 50.0% | |
| AGGCATGTCTACACCACTTG+TGG | - | chr1.1:70448684-70448703 | None:intergenic | 50.0% | |
| CTAGAATTGCAGGTCCACCA+GGG | - | chr1.1:70449201-70449220 | None:intergenic | 50.0% | |
| GATAGTGAAGGGGCCATTGA+TGG | + | chr1.1:70449034-70449053 | MS.gene88888:CDS | 50.0% | |
| GCAAGACATGGTGGTTGTAG+AGG | + | chr1.1:70448571-70448590 | MS.gene88888:CDS | 50.0% | |
| GGTACCTAGATTAGGGAAGG+AGG | + | chr1.1:70448592-70448611 | MS.gene88888:CDS | 50.0% | |
| TCAATGGCCCCTTCACTATC+TGG | - | chr1.1:70449034-70449053 | None:intergenic | 50.0% | |
| TGGCACCTTCATTGGATGCT+AGG | + | chr1.1:70448549-70448568 | MS.gene88888:CDS | 50.0% | |
| ! | ACTCACTTGGACAGCCTTGT+TGG | + | chr1.1:70448899-70448918 | MS.gene88888:CDS | 50.0% |
| ! | CAACAAGGTTGCTTTGCAGG+AGG | + | chr1.1:70448770-70448789 | MS.gene88888:CDS | 50.0% |
| ! | TCTAGAATTGCAGGTCCACC+AGG | - | chr1.1:70449202-70449221 | None:intergenic | 50.0% |
| ! | TTTTGGATTGCACACCCTGG+TGG | + | chr1.1:70449184-70449203 | MS.gene88888:CDS | 50.0% |
| !! | CAACAAAGGTGCTCGTGTGT+TGG | + | chr1.1:70448829-70448848 | MS.gene88888:CDS | 50.0% |
| !! | CAAGATGGACTGAAGACCAC+GGG | + | chr1.1:70449352-70449371 | MS.gene88888:CDS | 50.0% |
| !! | GTGAGTTGGTAATCAGCTCC+AGG | - | chr1.1:70448704-70448723 | None:intergenic | 50.0% |
| ACGGGAGAAGGACTTGAGTG+GGG | + | chr1.1:70449370-70449389 | MS.gene88888:CDS | 55.0% | |
| CACGGGAGAAGGACTTGAGT+GGG | + | chr1.1:70449369-70449388 | MS.gene88888:CDS | 55.0% | |
| CGCAATGCTCAAAGGGCAGA+AGG | + | chr1.1:70448181-70448200 | MS.gene88888:CDS | 55.0% | |
| CGGCCCTAGTGATACTCACT+TGG | + | chr1.1:70448886-70448905 | MS.gene88888:CDS | 55.0% | |
| GATGGTCACCTTCGTGAAGC+TGG | + | chr1.1:70449052-70449071 | MS.gene88888:CDS | 55.0% | |
| GCAGCCTCCTTCCCTAATCT+AGG | - | chr1.1:70448599-70448618 | None:intergenic | 55.0% | |
| GGATGCTAGGCAAGACATGG+TGG | + | chr1.1:70448562-70448581 | MS.gene88888:CDS | 55.0% | |
| GGCTGGGTTTGCAGTTCCAA+TGG | - | chr1.1:70448224-70448243 | None:intergenic | 55.0% | |
| ! | AGGTTGCTTTGCAGGAGGCA+CGG | + | chr1.1:70448775-70448794 | MS.gene88888:CDS | 55.0% |
| !! | GGAGCTGCTGCACTGATTGT+TGG | + | chr1.1:70448941-70448960 | MS.gene88888:CDS | 55.0% |
| CCACGGGAGAAGGACTTGAG+TGG | + | chr1.1:70449368-70449387 | MS.gene88888:CDS | 60.0% | |
| CCACTCAAGTCCTTCTCCCG+TGG | - | chr1.1:70449371-70449390 | None:intergenic | 60.0% | |
| GGACTGAAGACCACGGGAGA+AGG | + | chr1.1:70449358-70449377 | MS.gene88888:CDS | 60.0% | |
| ! | AGGAGGCACGGTGCTTCGTT+TGG | + | chr1.1:70448787-70448806 | MS.gene88888:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 70448160 | 70449459 | 70448160 | ID=MS.gene88888 |
| chr1.1 | mRNA | 70448160 | 70449459 | 70448160 | ID=MS.gene88888.t1;Parent=MS.gene88888 |
| chr1.1 | exon | 70448160 | 70448337 | 70448160 | ID=MS.gene88888.t1.exon1;Parent=MS.gene88888.t1 |
| chr1.1 | CDS | 70448160 | 70448337 | 70448160 | ID=cds.MS.gene88888.t1;Parent=MS.gene88888.t1 |
| chr1.1 | exon | 70448468 | 70449459 | 70448468 | ID=MS.gene88888.t1.exon2;Parent=MS.gene88888.t1 |
| chr1.1 | CDS | 70448468 | 70449459 | 70448468 | ID=cds.MS.gene88888.t1;Parent=MS.gene88888.t1 |
| Gene Sequence |
| Protein sequence |
