Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60721.t1 | XP_003592044.1 | 99.7 | 389 | 1 | 0 | 1 | 389 | 1 | 389 | 5.60E-221 | 776.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60721.t1 | P30075 | 98.5 | 389 | 6 | 0 | 1 | 389 | 1 | 389 | 3.7e-221 | 768.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60721.t1 | G7IAK9 | 99.7 | 389 | 1 | 0 | 1 | 389 | 1 | 389 | 4.0e-221 | 776.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049181 | MS.gene60721 | 0.807335 | 5.32E-50 | -1.69E-46 |
| MS.gene049259 | MS.gene60721 | 0.804751 | 1.86E-49 | -1.69E-46 |
| MS.gene049281 | MS.gene60721 | 0.800531 | 1.38E-48 | -1.69E-46 |
| MS.gene049320 | MS.gene60721 | -0.814991 | 1.16E-51 | -1.69E-46 |
| MS.gene05015 | MS.gene60721 | 0.81108 | 8.37E-51 | -1.69E-46 |
| MS.gene050216 | MS.gene60721 | -0.80089 | 1.17E-48 | -1.69E-46 |
| MS.gene050363 | MS.gene60721 | 0.823824 | 1.13E-53 | -1.69E-46 |
| MS.gene050381 | MS.gene60721 | 0.868016 | 8.93E-66 | -1.69E-46 |
| MS.gene050504 | MS.gene60721 | -0.82071 | 5.94E-53 | -1.69E-46 |
| MS.gene050612 | MS.gene60721 | -0.821875 | 3.20E-53 | -1.69E-46 |
| MS.gene050868 | MS.gene60721 | -0.816722 | 4.78E-52 | -1.69E-46 |
| MS.gene050871 | MS.gene60721 | -0.814322 | 1.63E-51 | -1.69E-46 |
| MS.gene050883 | MS.gene60721 | 0.939026 | 2.53E-99 | -1.69E-46 |
| MS.gene050986 | MS.gene60721 | -0.803167 | 3.97E-49 | -1.69E-46 |
| MS.gene051044 | MS.gene60721 | 0.840365 | 9.09E-58 | -1.69E-46 |
| MS.gene051127 | MS.gene60721 | 0.850574 | 1.56E-60 | -1.69E-46 |
| MS.gene051138 | MS.gene60721 | 0.822667 | 2.10E-53 | -1.69E-46 |
| MS.gene051364 | MS.gene60721 | 0.818168 | 2.26E-52 | -1.69E-46 |
| MS.gene051366 | MS.gene60721 | 0.804522 | 2.08E-49 | -1.69E-46 |
| MS.gene051410 | MS.gene60721 | 0.805834 | 1.10E-49 | -1.69E-46 |
| MS.gene05141 | MS.gene60721 | 0.824214 | 9.11E-54 | -1.69E-46 |
| MS.gene051946 | MS.gene60721 | 0.859282 | 4.61E-63 | -1.69E-46 |
| MS.gene052053 | MS.gene60721 | 0.83181 | 1.35E-55 | -1.69E-46 |
| MS.gene052482 | MS.gene60721 | -0.821843 | 3.25E-53 | -1.69E-46 |
| MS.gene052486 | MS.gene60721 | -0.813842 | 2.09E-51 | -1.69E-46 |
| MS.gene052802 | MS.gene60721 | 0.809885 | 1.52E-50 | -1.69E-46 |
| MS.gene053069 | MS.gene60721 | 0.812637 | 3.84E-51 | -1.69E-46 |
| MS.gene053420 | MS.gene60721 | 0.836127 | 1.12E-56 | -1.69E-46 |
| MS.gene053488 | MS.gene60721 | 0.822549 | 2.23E-53 | -1.69E-46 |
| MS.gene053549 | MS.gene60721 | 0.815351 | 9.67E-52 | -1.69E-46 |
| MS.gene053561 | MS.gene60721 | 0.845105 | 5.00E-59 | -1.69E-46 |
| MS.gene053826 | MS.gene60721 | 0.841922 | 3.54E-58 | -1.69E-46 |
| MS.gene054054 | MS.gene60721 | 0.809732 | 1.64E-50 | -1.69E-46 |
| MS.gene054056 | MS.gene60721 | 0.836723 | 7.92E-57 | -1.69E-46 |
| MS.gene054616 | MS.gene60721 | 0.803333 | 3.67E-49 | -1.69E-46 |
| MS.gene054707 | MS.gene60721 | 0.8022 | 6.28E-49 | -1.69E-46 |
| MS.gene054708 | MS.gene60721 | 0.836626 | 8.38E-57 | -1.69E-46 |
| MS.gene054954 | MS.gene60721 | -0.804199 | 2.42E-49 | -1.69E-46 |
| MS.gene055124 | MS.gene60721 | 0.84245 | 2.57E-58 | -1.69E-46 |
| MS.gene055139 | MS.gene60721 | 0.812963 | 3.25E-51 | -1.69E-46 |
| MS.gene055154 | MS.gene60721 | 0.865952 | 4.06E-65 | -1.69E-46 |
| MS.gene055202 | MS.gene60721 | 0.813183 | 2.91E-51 | -1.69E-46 |
| MS.gene055362 | MS.gene60721 | -0.804607 | 1.99E-49 | -1.69E-46 |
| MS.gene055502 | MS.gene60721 | -0.815363 | 9.60E-52 | -1.69E-46 |
| MS.gene055549 | MS.gene60721 | 0.852452 | 4.57E-61 | -1.69E-46 |
| MS.gene055550 | MS.gene60721 | 0.850259 | 1.91E-60 | -1.69E-46 |
| MS.gene055551 | MS.gene60721 | 0.827304 | 1.68E-54 | -1.69E-46 |
| MS.gene05589 | MS.gene60721 | -0.808979 | 2.37E-50 | -1.69E-46 |
| MS.gene055942 | MS.gene60721 | 0.816958 | 4.23E-52 | -1.69E-46 |
| MS.gene055961 | MS.gene60721 | 0.816479 | 5.42E-52 | -1.69E-46 |
| MS.gene056224 | MS.gene60721 | 0.804931 | 1.70E-49 | -1.69E-46 |
| MS.gene056310 | MS.gene60721 | 0.88168 | 1.96E-70 | -1.69E-46 |
| MS.gene056311 | MS.gene60721 | 0.918728 | 1.09E-86 | -1.69E-46 |
| MS.gene056313 | MS.gene60721 | 0.856736 | 2.63E-62 | -1.69E-46 |
| MS.gene056365 | MS.gene60721 | 0.81468 | 1.36E-51 | -1.69E-46 |
| MS.gene056504 | MS.gene60721 | -0.834521 | 2.86E-56 | -1.69E-46 |
| MS.gene056530 | MS.gene60721 | 0.830297 | 3.18E-55 | -1.69E-46 |
| MS.gene057014 | MS.gene60721 | 0.883164 | 5.66E-71 | -1.69E-46 |
| MS.gene058099 | MS.gene60721 | 0.846828 | 1.70E-59 | -1.69E-46 |
| MS.gene058108 | MS.gene60721 | 0.829551 | 4.83E-55 | -1.69E-46 |
| MS.gene058109 | MS.gene60721 | 0.880276 | 6.27E-70 | -1.69E-46 |
| MS.gene058111 | MS.gene60721 | 0.903136 | 4.74E-79 | -1.69E-46 |
| MS.gene058117 | MS.gene60721 | 0.803049 | 4.20E-49 | -1.69E-46 |
| MS.gene058171 | MS.gene60721 | 0.886188 | 4.25E-72 | -1.69E-46 |
| MS.gene058173 | MS.gene60721 | 0.887329 | 1.57E-72 | -1.69E-46 |
| MS.gene058175 | MS.gene60721 | 0.891588 | 3.46E-74 | -1.69E-46 |
| MS.gene058268 | MS.gene60721 | 0.80288 | 4.55E-49 | -1.69E-46 |
| MS.gene058493 | MS.gene60721 | 0.871756 | 5.36E-67 | -1.69E-46 |
| MS.gene058494 | MS.gene60721 | 0.878043 | 3.86E-69 | -1.69E-46 |
| MS.gene058496 | MS.gene60721 | 0.831721 | 1.42E-55 | -1.69E-46 |
| MS.gene058628 | MS.gene60721 | 0.868541 | 6.05E-66 | -1.69E-46 |
| MS.gene058629 | MS.gene60721 | 0.854421 | 1.24E-61 | -1.69E-46 |
| MS.gene058630 | MS.gene60721 | 0.859791 | 3.24E-63 | -1.69E-46 |
| MS.gene05884 | MS.gene60721 | 0.809384 | 1.94E-50 | -1.69E-46 |
| MS.gene059022 | MS.gene60721 | -0.825526 | 4.47E-54 | -1.69E-46 |
| MS.gene059045 | MS.gene60721 | 0.822904 | 1.85E-53 | -1.69E-46 |
| MS.gene059223 | MS.gene60721 | 0.827988 | 1.15E-54 | -1.69E-46 |
| MS.gene059690 | MS.gene60721 | 0.872168 | 3.91E-67 | -1.69E-46 |
| MS.gene059691 | MS.gene60721 | 0.841101 | 5.83E-58 | -1.69E-46 |
| MS.gene059694 | MS.gene60721 | 0.874594 | 5.98E-68 | -1.69E-46 |
| MS.gene059697 | MS.gene60721 | 0.872431 | 3.20E-67 | -1.69E-46 |
| MS.gene059865 | MS.gene60721 | -0.832558 | 8.83E-56 | -1.69E-46 |
| MS.gene060595 | MS.gene60721 | 0.809503 | 1.83E-50 | -1.69E-46 |
| MS.gene06077 | MS.gene60721 | -0.83335 | 5.61E-56 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60721.t1 | MTR_1g098140 | 99.743 | 389 | 1 | 0 | 1 | 389 | 1 | 389 | 0.0 | 803 |
| MS.gene60721.t1 | MTR_1g097935 | 99.486 | 389 | 2 | 0 | 1 | 389 | 1 | 389 | 0.0 | 800 |
| MS.gene60721.t1 | MTR_1g097910 | 99.229 | 389 | 3 | 0 | 1 | 389 | 1 | 389 | 0.0 | 799 |
| MS.gene60721.t1 | MTR_7g016800 | 98.458 | 389 | 6 | 0 | 1 | 389 | 1 | 389 | 0.0 | 794 |
| MS.gene60721.t1 | MTR_7g016720 | 98.458 | 389 | 6 | 0 | 1 | 389 | 1 | 389 | 0.0 | 792 |
| MS.gene60721.t1 | MTR_7g016820 | 98.201 | 389 | 7 | 0 | 1 | 389 | 1 | 389 | 0.0 | 791 |
| MS.gene60721.t1 | MTR_5g007730 | 97.686 | 389 | 9 | 0 | 1 | 389 | 1 | 389 | 0.0 | 790 |
| MS.gene60721.t1 | MTR_7g016700 | 97.686 | 389 | 9 | 0 | 1 | 389 | 1 | 389 | 0.0 | 790 |
| MS.gene60721.t1 | MTR_7g016780 | 97.686 | 389 | 9 | 0 | 1 | 389 | 1 | 389 | 0.0 | 788 |
| MS.gene60721.t1 | MTR_5g007760 | 97.429 | 389 | 10 | 0 | 1 | 389 | 1 | 389 | 0.0 | 787 |
| MS.gene60721.t1 | MTR_5g007713 | 96.401 | 389 | 14 | 0 | 1 | 389 | 1 | 389 | 0.0 | 781 |
| MS.gene60721.t1 | MTR_5g007717 | 96.144 | 389 | 15 | 0 | 1 | 389 | 1 | 389 | 0.0 | 780 |
| MS.gene60721.t1 | MTR_3g083920 | 90.722 | 388 | 36 | 0 | 1 | 388 | 1 | 388 | 0.0 | 748 |
| MS.gene60721.t1 | MTR_3g083910 | 89.948 | 388 | 39 | 0 | 1 | 388 | 1 | 388 | 0.0 | 744 |
| MS.gene60721.t1 | MTR_2g058470 | 87.147 | 389 | 50 | 0 | 1 | 389 | 1 | 389 | 0.0 | 713 |
| MS.gene60721.t1 | MTR_3g086260 | 81.701 | 388 | 71 | 0 | 1 | 388 | 1 | 388 | 0.0 | 676 |
| MS.gene60721.t1 | MTR_7g084300 | 80.206 | 389 | 77 | 0 | 1 | 389 | 1 | 389 | 0.0 | 670 |
| MS.gene60721.t1 | MTR_7g113405 | 96.040 | 303 | 12 | 0 | 87 | 389 | 1 | 303 | 0.0 | 597 |
| MS.gene60721.t1 | MTR_3g088675 | 66.067 | 389 | 132 | 0 | 1 | 389 | 1 | 389 | 0.0 | 572 |
| MS.gene60721.t1 | MTR_5g007740 | 80.061 | 326 | 65 | 0 | 64 | 389 | 1 | 326 | 0.0 | 546 |
| MS.gene60721.t1 | MTR_5g007723 | 68.514 | 397 | 76 | 5 | 12 | 389 | 32 | 398 | 0.0 | 530 |
| MS.gene60721.t1 | MTR_5g007770 | 77.667 | 300 | 34 | 2 | 70 | 369 | 1 | 267 | 3.47e-166 | 466 |
| MS.gene60721.t1 | MTR_3g088670 | 49.231 | 390 | 184 | 6 | 10 | 388 | 2 | 388 | 1.11e-131 | 383 |
| MS.gene60721.t1 | MTR_4g075560 | 42.632 | 380 | 211 | 4 | 11 | 387 | 13 | 388 | 1.35e-102 | 309 |
| MS.gene60721.t1 | MTR_4g078730 | 37.802 | 373 | 225 | 2 | 15 | 387 | 24 | 389 | 2.72e-94 | 288 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60721.t1 | AT5G13930 | 82.474 | 388 | 67 | 1 | 3 | 389 | 8 | 395 | 0.0 | 671 |
| MS.gene60721.t1 | AT4G34850 | 40.849 | 377 | 219 | 3 | 15 | 389 | 18 | 392 | 1.78e-97 | 296 |
| MS.gene60721.t1 | AT4G00040 | 40.584 | 377 | 214 | 5 | 14 | 387 | 16 | 385 | 2.37e-96 | 293 |
| MS.gene60721.t1 | AT1G02050 | 39.894 | 376 | 216 | 5 | 15 | 387 | 24 | 392 | 2.19e-88 | 273 |
| MS.gene60721.t1 | AT4G34850 | 40.312 | 320 | 188 | 2 | 72 | 389 | 1 | 319 | 5.35e-82 | 254 |
| MS.gene60721.t1 | AT3G52160 | 26.174 | 298 | 192 | 12 | 103 | 387 | 42 | 324 | 5.65e-12 | 67.0 |
| MS.gene60721.t1 | AT3G52160 | 26.174 | 298 | 192 | 13 | 103 | 387 | 155 | 437 | 1.11e-11 | 66.6 |
Find 88 sgRNAs with CRISPR-Local
Find 115 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAATAGGTTTCTCAATTTC+TGG | 0.101255 | 1.2:+71208173 | None:intergenic |
| TGCAGTTCCAATGGCTAAAA+TGG | 0.225262 | 1.2:+71208930 | None:intergenic |
| CTGATTACAATTCAATCTTT+TGG | 0.254027 | 1.2:-71207983 | MS.gene60721:CDS |
| CCTTTGAGCATTGCGAATTT+CGG | 0.276950 | 1.2:+71208969 | None:intergenic |
| TACCAACAAGGTTGCTTTGC+AGG | 0.283953 | 1.2:-71208383 | MS.gene60721:CDS |
| CTTTCAAAATCTCCTCTGTT+AGG | 0.305425 | 1.2:+71208648 | None:intergenic |
| CTTGTTGGACAAGCACTATT+TGG | 0.314421 | 1.2:-71208236 | MS.gene60721:CDS |
| AGAGAAGTGCTCAGTGAATA+TGG | 0.317887 | 1.2:-71207879 | MS.gene60721:CDS |
| GCTTCGTTTGGCTAAAGATT+TGG | 0.342769 | 1.2:-71208351 | MS.gene60721:CDS |
| GTCTTCGTCCATACGTAAAA+AGG | 0.344471 | 1.2:-71208415 | MS.gene60721:CDS |
| TCTACTTGATCTAGAATTGC+AGG | 0.346751 | 1.2:+71207942 | None:intergenic |
| TGAATACATGGCACCTTCAT+TGG | 0.347646 | 1.2:-71208609 | MS.gene60721:CDS |
| TCAATGGCTCCTTCACTATC+TGG | 0.348362 | 1.2:+71208119 | None:intergenic |
| GGTTTCTCAATTTCTGGTAC+TGG | 0.362281 | 1.2:+71208179 | None:intergenic |
| AAGTGAGTAATCTTTGACTT+TGG | 0.381814 | 1.2:+71208503 | None:intergenic |
| AGGAGGCACGGTGCTTCGTT+TGG | 0.381968 | 1.2:-71208363 | MS.gene60721:CDS |
| TACCAACTCACAAAACTCTT+AGG | 0.382277 | 1.2:-71208437 | MS.gene60721:CDS |
| CCAATGGCTAAAATGGTTGC+AGG | 0.393820 | 1.2:+71208937 | None:intergenic |
| ATATTCACTGAGCACTTCTC+TGG | 0.410278 | 1.2:+71207881 | None:intergenic |
| GGAGCTGCTGCACTGATTGT+TGG | 0.411529 | 1.2:-71208209 | MS.gene60721:CDS |
| CACCTTCTAAAAGATGTTCC+CGG | 0.414732 | 1.2:-71208065 | MS.gene60721:CDS |
| ACTCACTTGGACAGCCTTGT+TGG | 0.426712 | 1.2:-71208251 | MS.gene60721:CDS |
| AGTGAAAGCTATAAAAGAAT+GGG | 0.430526 | 1.2:-71208531 | MS.gene60721:CDS |
| CTTTGTTCAACACAGTTGGC+TGG | 0.449203 | 1.2:+71208904 | None:intergenic |
| GTCACTGCAGTTACATTTCG+CGG | 0.450823 | 1.2:-71208284 | MS.gene60721:CDS |
| CCGAAATTCGCAATGCTCAA+AGG | 0.469903 | 1.2:-71208969 | MS.gene60721:CDS |
| CGAAATTCGCAATGCTCAAA+GGG | 0.470864 | 1.2:-71208968 | MS.gene60721:CDS |
| TTCACGAAGGTGACCATCAA+TGG | 0.471758 | 1.2:+71208103 | None:intergenic |
| GCAAGACATGGTGGTGGTAG+AGG | 0.474656 | 1.2:-71208579 | MS.gene60721:CDS |
| CTCCTGCAAAGCAACCTTGT+TGG | 0.478750 | 1.2:+71208381 | None:intergenic |
| GTGAGTTGGTAATCAGCTCC+AGG | 0.479782 | 1.2:+71208449 | None:intergenic |
| GATAGTGAAGGAGCCATTGA+TGG | 0.482535 | 1.2:-71208116 | MS.gene60721:CDS |
| CAGTGAAAGCTATAAAAGAA+TGG | 0.482912 | 1.2:-71208532 | MS.gene60721:CDS |
| CTGTCCAAGTGAGTATCACT+AGG | 0.483602 | 1.2:+71208260 | None:intergenic |
| AAAGAACATCAATAAAGCAT+TGG | 0.485622 | 1.2:-71208033 | MS.gene60721:CDS |
| GATGGTCACCTTCGTGAAGC+TGG | 0.486168 | 1.2:-71208098 | MS.gene60721:CDS |
| TCTAGAATTGCAGGTCCACC+AGG | 0.500155 | 1.2:+71207951 | None:intergenic |
| TTGCGCAGTGTCGCTATATG+AGG | 0.502378 | 1.2:-71207711 | None:intergenic |
| GGCTGGATTTGCAGTTCCAA+TGG | 0.506046 | 1.2:+71208921 | None:intergenic |
| TGGCACCTTCATTGGATGCT+AGG | 0.506096 | 1.2:-71208601 | MS.gene60721:CDS |
| GATGTTCTTTGAAACAATCC+CGG | 0.506109 | 1.2:+71208046 | None:intergenic |
| GCGCAAAACAACAGTTTCAA+TGG | 0.506548 | 1.2:+71207728 | None:intergenic |
| ACCTTCTAAAAGATGTTCCC+GGG | 0.518985 | 1.2:-71208064 | MS.gene60721:CDS |
| TTCACTGAGCACTTCTCTGG+TGG | 0.523654 | 1.2:+71207884 | None:intergenic |
| GGACTGAAGACCACGGGAGA+AGG | 0.524843 | 1.2:-71207792 | MS.gene60721:CDS |
| TCCTAGTGTTTGTGAATACA+TGG | 0.528394 | 1.2:-71208621 | MS.gene60721:CDS |
| CGGCCCTAGTGATACTCACT+TGG | 0.529910 | 1.2:-71208264 | MS.gene60721:CDS |
| CATCAATAAAGCATTGGTTG+AGG | 0.540608 | 1.2:-71208027 | MS.gene60721:CDS |
| ATGTTCTTTGAAACAATCCC+GGG | 0.547822 | 1.2:+71208047 | None:intergenic |
| CGCAATGCTCAAAGGGCAGA+AGG | 0.548900 | 1.2:-71208961 | MS.gene60721:CDS |
| AGGTTGCTTTGCAGGAGGCA+CGG | 0.555669 | 1.2:-71208375 | MS.gene60721:CDS |
| GGACAAGCACTATTTGGAGA+TGG | 0.563405 | 1.2:-71208230 | MS.gene60721:CDS |
| AGGCATGTCTACACCACTTG+TGG | 0.569916 | 1.2:+71208469 | None:intergenic |
| ACAAGTGGTGTAGACATGCC+TGG | 0.572074 | 1.2:-71208467 | MS.gene60721:CDS |
| CACGGGAGAAGGACTTGAGT+GGG | 0.572386 | 1.2:-71207781 | MS.gene60721:CDS |
| CAAATAGTGCTTGTCCAACA+AGG | 0.572762 | 1.2:+71208237 | None:intergenic |
| TCTTGCCTAGCATCCAATGA+AGG | 0.579296 | 1.2:+71208596 | None:intergenic |
| GTGATAAATCTATGATCAAG+AGG | 0.584564 | 1.2:-71208682 | MS.gene60721:CDS |
| TTGATCGTTTGCACCACAAG+TGG | 0.595788 | 1.2:-71208482 | MS.gene60721:CDS |
| AATGAGAAAGAAGTCCGCTC+AGG | 0.596562 | 1.2:-71207817 | MS.gene60721:CDS |
| AGAAAGAAGTCCGCTCAGGA+TGG | 0.597434 | 1.2:-71207813 | MS.gene60721:CDS |
| AGAGGTACTTAGACTAGGCA+AGG | 0.600359 | 1.2:-71208561 | MS.gene60721:CDS |
| CAGGATGGACTGAAGACCAC+GGG | 0.604373 | 1.2:-71207798 | MS.gene60721:CDS |
| CCACTCAAGTCCTTCTCCCG+TGG | 0.610390 | 1.2:+71207782 | None:intergenic |
| TGTGCTTTGTTCAACACAGT+TGG | 0.612931 | 1.2:+71208900 | None:intergenic |
| GTGGTAGAGGTACTTAGACT+AGG | 0.615806 | 1.2:-71208566 | MS.gene60721:CDS |
| TTGTAATCAGAAATTCCCAA+TGG | 0.619803 | 1.2:+71207996 | None:intergenic |
| CAACAAAGGTGCTCGTGTGT+TGG | 0.620198 | 1.2:-71208321 | MS.gene60721:CDS |
| CAACAAGGTTGCTTTGCAGG+AGG | 0.634702 | 1.2:-71208380 | MS.gene60721:CDS |
| CCACGGGAGAAGGACTTGAG+TGG | 0.645541 | 1.2:-71207782 | MS.gene60721:CDS |
| ATTGGATGCTAGGCAAGACA+TGG | 0.648366 | 1.2:-71208591 | MS.gene60721:CDS |
| CAATGGCTAAAATGGTTGCA+GGG | 0.655281 | 1.2:+71208938 | None:intergenic |
| ACAATTGCTCCAGATAGTGA+AGG | 0.659707 | 1.2:-71208128 | MS.gene60721:CDS |
| GTGAAAGCTATAAAAGAATG+GGG | 0.667253 | 1.2:-71208530 | MS.gene60721:CDS |
| GAGATACATGTACCTAACAG+AGG | 0.667740 | 1.2:-71208660 | MS.gene60721:CDS |
| TGTCCAAGTGAGTATCACTA+GGG | 0.674277 | 1.2:+71208261 | None:intergenic |
| TGCTAGGCAAGACATGGTGG+TGG | 0.675229 | 1.2:-71208585 | MS.gene60721:CDS |
| GGTCTTCAGTCCATCCTGAG+CGG | 0.678236 | 1.2:+71207803 | None:intergenic |
| ACAGTTTCAATGGTAAGACC+CGG | 0.678772 | 1.2:+71207738 | None:intergenic |
| TCAGGATGGACTGAAGACCA+CGG | 0.679433 | 1.2:-71207799 | MS.gene60721:CDS |
| GCCATGTATTCACAAACACT+AGG | 0.680230 | 1.2:+71208620 | None:intergenic |
| GATTTGGCTGAGAACAACAA+AGG | 0.683242 | 1.2:-71208335 | MS.gene60721:CDS |
| GGTACTTAGACTAGGCAAGG+AGG | 0.684618 | 1.2:-71208558 | MS.gene60721:CDS |
| ATGTTAGTCCAGCTTCACGA+AGG | 0.698828 | 1.2:+71208090 | None:intergenic |
| AGGTATATGATGTACCAACA+AGG | 0.704257 | 1.2:-71208395 | MS.gene60721:CDS |
| CTAGAATTGCAGGTCCACCA+GGG | 0.711261 | 1.2:+71207952 | None:intergenic |
| ACGGGAGAAGGACTTGAGTG+GGG | 0.725736 | 1.2:-71207780 | MS.gene60721:CDS |
| GGATGCTAGGCAAGACATGG+TGG | 0.737305 | 1.2:-71208588 | MS.gene60721:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GATAGCAAAATATATTTAAA+TGG | + | chr1.2:71207928-71207947 | None:intergenic | 15.0% |
| !!! | ATATTTTGCTATCTTTACAT+TGG | - | chr1.2:71207935-71207954 | MS.gene60721:CDS | 20.0% |
| !!! | ATTTAAATGGAAAGTATCTA+AGG | + | chr1.2:71207915-71207934 | None:intergenic | 20.0% |
| ! | AAAATAGGTTTCTCAATTTC+TGG | + | chr1.2:71208525-71208544 | None:intergenic | 25.0% |
| ! | AAAGAACATCAATAAAGCAT+TGG | - | chr1.2:71208662-71208681 | MS.gene60721:CDS | 25.0% |
| ! | AGTGAAAGCTATAAAAGAAT+GGG | - | chr1.2:71208164-71208183 | MS.gene60721:CDS | 25.0% |
| !! | ATCATATACCTTTTTACGTA+TGG | + | chr1.2:71208291-71208310 | None:intergenic | 25.0% |
| !! | CTGATTACAATTCAATCTTT+TGG | - | chr1.2:71208712-71208731 | MS.gene60721:intron | 25.0% |
| !!! | GTGATTTTAAAGTAGAAATC+AGG | + | chr1.2:71207824-71207843 | None:intergenic | 25.0% |
| ATCATAGATTTATCACCTGA+AGG | + | chr1.2:71208009-71208028 | None:intergenic | 30.0% | |
| CAGTGAAAGCTATAAAAGAA+TGG | - | chr1.2:71208163-71208182 | MS.gene60721:CDS | 30.0% | |
| GATATTAGTTCAGCAAACTT+GGG | + | chr1.2:71207971-71207990 | None:intergenic | 30.0% | |
| GTGAAAGCTATAAAAGAATG+GGG | - | chr1.2:71208165-71208184 | MS.gene60721:CDS | 30.0% | |
| GTGATAAATCTATGATCAAG+AGG | - | chr1.2:71208013-71208032 | MS.gene60721:CDS | 30.0% | |
| TCATAGATTTATCACCTGAA+GGG | + | chr1.2:71208008-71208027 | None:intergenic | 30.0% | |
| TGATATTAGTTCAGCAAACT+TGG | + | chr1.2:71207972-71207991 | None:intergenic | 30.0% | |
| TTGTAATCAGAAATTCCCAA+TGG | + | chr1.2:71208702-71208721 | None:intergenic | 30.0% | |
| ! | AAGTGAGTAATCTTTGACTT+TGG | + | chr1.2:71208195-71208214 | None:intergenic | 30.0% |
| ! | ATCTTTTCATAATTCCCTTC+AGG | - | chr1.2:71207991-71208010 | MS.gene60721:CDS | 30.0% |
| !! | TTTCATCTTTTCAGGCTTTA+AGG | + | chr1.2:71208790-71208809 | None:intergenic | 30.0% |
| !!! | AACCTATTTTTGAGATGGTT+TGG | - | chr1.2:71208535-71208554 | MS.gene60721:CDS | 30.0% |
| !!! | TGAGAAACCTATTTTTGAGA+TGG | - | chr1.2:71208530-71208549 | MS.gene60721:CDS | 30.0% |
| AGGTATATGATGTACCAACA+AGG | - | chr1.2:71208300-71208319 | MS.gene60721:CDS | 35.0% | |
| CTTTCAAAATCTCCTCTGTT+AGG | + | chr1.2:71208050-71208069 | None:intergenic | 35.0% | |
| GTCCAAACCATCTCAAAAAT+AGG | + | chr1.2:71208540-71208559 | None:intergenic | 35.0% | |
| TACCAACTCACAAAACTCTT+AGG | - | chr1.2:71208258-71208277 | MS.gene60721:CDS | 35.0% | |
| TCCTAGTGTTTGTGAATACA+TGG | - | chr1.2:71208074-71208093 | MS.gene60721:CDS | 35.0% | |
| ! | TCTACTTGATCTAGAATTGC+AGG | + | chr1.2:71208756-71208775 | None:intergenic | 35.0% |
| !! | ATGGAAAGTATCTAAGGTAG+AGG | + | chr1.2:71207909-71207928 | None:intergenic | 35.0% |
| !! | ATGTTCTTTGAAACAATCCC+GGG | + | chr1.2:71208651-71208670 | None:intergenic | 35.0% |
| !! | CATCAATAAAGCATTGGTTG+AGG | - | chr1.2:71208668-71208687 | MS.gene60721:CDS | 35.0% |
| !! | GATGTTCTTTGAAACAATCC+CGG | + | chr1.2:71208652-71208671 | None:intergenic | 35.0% |
| !!! | TGTTTTATTTGGGTTTGGAC+CGG | - | chr1.2:71208938-71208957 | MS.gene60721:CDS | 35.0% |
| !!! | TTGAGTGGGGTGTTTTATTT+GGG | - | chr1.2:71208928-71208947 | MS.gene60721:CDS | 35.0% |
| ACAATTGCTCCAGATAGTGA+AGG | - | chr1.2:71208567-71208586 | MS.gene60721:CDS | 40.0% | |
| ACAGTTTCAATGGTAAGACC+CGG | + | chr1.2:71208960-71208979 | None:intergenic | 40.0% | |
| ATATTCACTGAGCACTTCTC+TGG | + | chr1.2:71208817-71208836 | None:intergenic | 40.0% | |
| CAAATAGTGCTTGTCCAACA+AGG | + | chr1.2:71208461-71208480 | None:intergenic | 40.0% | |
| CAATGGCTAAAATGGTTGCA+GGG | + | chr1.2:71207760-71207779 | None:intergenic | 40.0% | |
| CGAAATTCGCAATGCTCAAA+GGG | - | chr1.2:71207727-71207746 | MS.gene60721:CDS | 40.0% | |
| CTTGTTGGACAAGCACTATT+TGG | - | chr1.2:71208459-71208478 | MS.gene60721:CDS | 40.0% | |
| GAGATACATGTACCTAACAG+AGG | - | chr1.2:71208035-71208054 | MS.gene60721:CDS | 40.0% | |
| GATTTGGCTGAGAACAACAA+AGG | - | chr1.2:71208360-71208379 | MS.gene60721:CDS | 40.0% | |
| GCCATGTATTCACAAACACT+AGG | + | chr1.2:71208078-71208097 | None:intergenic | 40.0% | |
| GCGCAAAACAACAGTTTCAA+TGG | + | chr1.2:71208970-71208989 | None:intergenic | 40.0% | |
| GCTTCGTTTGGCTAAAGATT+TGG | - | chr1.2:71208344-71208363 | MS.gene60721:CDS | 40.0% | |
| GTCTTCGTCCATACGTAAAA+AGG | - | chr1.2:71208280-71208299 | MS.gene60721:CDS | 40.0% | |
| TGAATACATGGCACCTTCAT+TGG | - | chr1.2:71208086-71208105 | MS.gene60721:CDS | 40.0% | |
| TGCAGTTCCAATGGCTAAAA+TGG | + | chr1.2:71207768-71207787 | None:intergenic | 40.0% | |
| TGTCCAAGTGAGTATCACTA+GGG | + | chr1.2:71208437-71208456 | None:intergenic | 40.0% | |
| ! | ACCTTCTAAAAGATGTTCCC+GGG | - | chr1.2:71208631-71208650 | MS.gene60721:CDS | 40.0% |
| ! | CACCTTCTAAAAGATGTTCC+CGG | - | chr1.2:71208630-71208649 | MS.gene60721:CDS | 40.0% |
| ! | CCTTTGAGCATTGCGAATTT+CGG | + | chr1.2:71207729-71207748 | None:intergenic | 40.0% |
| ! | GTTGAGGCTTTTGAACCATT+GGG | - | chr1.2:71208684-71208703 | MS.gene60721:CDS | 40.0% |
| ! | TGTGCTTTGTTCAACACAGT+TGG | + | chr1.2:71207798-71207817 | None:intergenic | 40.0% |
| !! | AGAGAAGTGCTCAGTGAATA+TGG | - | chr1.2:71208816-71208835 | MS.gene60721:intron | 40.0% |
| !! | CTTGAGTGGGGTGTTTTATT+TGG | - | chr1.2:71208927-71208946 | MS.gene60721:CDS | 40.0% |
| !! | GACCTAAGAGTTTTGTGAGT+TGG | + | chr1.2:71208263-71208282 | None:intergenic | 40.0% |
| !! | GGTTTCTCAATTTCTGGTAC+TGG | + | chr1.2:71208519-71208538 | None:intergenic | 40.0% |
| !!! | GTTTTATTTGGGTTTGGACC+GGG | - | chr1.2:71208939-71208958 | MS.gene60721:CDS | 40.0% |
| !!! | TGGGGTGTTTTATTTGGGTT+TGG | - | chr1.2:71208933-71208952 | MS.gene60721:CDS | 40.0% |
| AATGAGAAAGAAGTCCGCTC+AGG | - | chr1.2:71208878-71208897 | MS.gene60721:CDS | 45.0% | |
| ATGTTAGTCCAGCTTCACGA+AGG | + | chr1.2:71208608-71208627 | None:intergenic | 45.0% | |
| ATTGGATGCTAGGCAAGACA+TGG | - | chr1.2:71208104-71208123 | MS.gene60721:CDS | 45.0% | |
| CCAATGGCTAAAATGGTTGC+AGG | + | chr1.2:71207761-71207780 | None:intergenic | 45.0% | |
| CCGAAATTCGCAATGCTCAA+AGG | - | chr1.2:71207726-71207745 | MS.gene60721:CDS | 45.0% | |
| CTGTCCAAGTGAGTATCACT+AGG | + | chr1.2:71208438-71208457 | None:intergenic | 45.0% | |
| CTTTGTTCAACACAGTTGGC+TGG | + | chr1.2:71207794-71207813 | None:intergenic | 45.0% | |
| GATAGTGAAGGAGCCATTGA+TGG | - | chr1.2:71208579-71208598 | MS.gene60721:CDS | 45.0% | |
| GGACAAGCACTATTTGGAGA+TGG | - | chr1.2:71208465-71208484 | MS.gene60721:CDS | 45.0% | |
| GTCACTGCAGTTACATTTCG+CGG | - | chr1.2:71208411-71208430 | MS.gene60721:CDS | 45.0% | |
| TCAATGGCTCCTTCACTATC+TGG | + | chr1.2:71208579-71208598 | None:intergenic | 45.0% | |
| TCTTGCCTAGCATCCAATGA+AGG | + | chr1.2:71208102-71208121 | None:intergenic | 45.0% | |
| TTCACGAAGGTGACCATCAA+TGG | + | chr1.2:71208595-71208614 | None:intergenic | 45.0% | |
| TTGATCGTTTGCACCACAAG+TGG | - | chr1.2:71208213-71208232 | MS.gene60721:CDS | 45.0% | |
| ! | ATCTTTTGGATTGCACACCC+TGG | - | chr1.2:71208726-71208745 | MS.gene60721:intron | 45.0% |
| ! | CTGGTGGCTTTCATCTTTTC+AGG | + | chr1.2:71208798-71208817 | None:intergenic | 45.0% |
| ! | GGTTGAGGCTTTTGAACCAT+TGG | - | chr1.2:71208683-71208702 | MS.gene60721:CDS | 45.0% |
| ! | TACCAACAAGGTTGCTTTGC+AGG | - | chr1.2:71208312-71208331 | MS.gene60721:CDS | 45.0% |
| ! | TCCCGGGAACATCTTTTAGA+AGG | + | chr1.2:71208635-71208654 | None:intergenic | 45.0% |
| !! | AGAGGTACTTAGACTAGGCA+AGG | - | chr1.2:71208134-71208153 | MS.gene60721:CDS | 45.0% |
| !! | CCTGCAACCATTTTAGCCAT+TGG | - | chr1.2:71207758-71207777 | MS.gene60721:CDS | 45.0% |
| !! | GTGGTAGAGGTACTTAGACT+AGG | - | chr1.2:71208129-71208148 | MS.gene60721:CDS | 45.0% |
| ACAAGTGGTGTAGACATGCC+TGG | - | chr1.2:71208228-71208247 | MS.gene60721:CDS | 50.0% | |
| AGAAAGAAGTCCGCTCAGGA+TGG | - | chr1.2:71208882-71208901 | MS.gene60721:CDS | 50.0% | |
| AGGCATGTCTACACCACTTG+TGG | + | chr1.2:71208229-71208248 | None:intergenic | 50.0% | |
| CTAGAATTGCAGGTCCACCA+GGG | + | chr1.2:71208746-71208765 | None:intergenic | 50.0% | |
| CTCCTGCAAAGCAACCTTGT+TGG | + | chr1.2:71208317-71208336 | None:intergenic | 50.0% | |
| GGCTGGATTTGCAGTTCCAA+TGG | + | chr1.2:71207777-71207796 | None:intergenic | 50.0% | |
| GGTACTTAGACTAGGCAAGG+AGG | - | chr1.2:71208137-71208156 | MS.gene60721:CDS | 50.0% | |
| TGGCACCTTCATTGGATGCT+AGG | - | chr1.2:71208094-71208113 | MS.gene60721:CDS | 50.0% | |
| TTCACTGAGCACTTCTCTGG+TGG | + | chr1.2:71208814-71208833 | None:intergenic | 50.0% | |
| ! | ACTCACTTGGACAGCCTTGT+TGG | - | chr1.2:71208444-71208463 | MS.gene60721:CDS | 50.0% |
| ! | CAACAAGGTTGCTTTGCAGG+AGG | - | chr1.2:71208315-71208334 | MS.gene60721:CDS | 50.0% |
| ! | TCTAGAATTGCAGGTCCACC+AGG | + | chr1.2:71208747-71208766 | None:intergenic | 50.0% |
| ! | TTTTGGATTGCACACCCTGG+TGG | - | chr1.2:71208729-71208748 | MS.gene60721:intron | 50.0% |
| !! | CAACAAAGGTGCTCGTGTGT+TGG | - | chr1.2:71208374-71208393 | MS.gene60721:CDS | 50.0% |
| !! | GTGAGTTGGTAATCAGCTCC+AGG | + | chr1.2:71208249-71208268 | None:intergenic | 50.0% |
| !! | TCAGGATGGACTGAAGACCA+CGG | - | chr1.2:71208896-71208915 | MS.gene60721:CDS | 50.0% |
| ACGGGAGAAGGACTTGAGTG+GGG | - | chr1.2:71208915-71208934 | MS.gene60721:CDS | 55.0% | |
| CACGGGAGAAGGACTTGAGT+GGG | - | chr1.2:71208914-71208933 | MS.gene60721:CDS | 55.0% | |
| CGCAATGCTCAAAGGGCAGA+AGG | - | chr1.2:71207734-71207753 | MS.gene60721:CDS | 55.0% | |
| CGGCCCTAGTGATACTCACT+TGG | - | chr1.2:71208431-71208450 | MS.gene60721:CDS | 55.0% | |
| GATGGTCACCTTCGTGAAGC+TGG | - | chr1.2:71208597-71208616 | MS.gene60721:CDS | 55.0% | |
| GGATGCTAGGCAAGACATGG+TGG | - | chr1.2:71208107-71208126 | MS.gene60721:CDS | 55.0% | |
| GGTCTTCAGTCCATCCTGAG+CGG | + | chr1.2:71208895-71208914 | None:intergenic | 55.0% | |
| TGCTAGGCAAGACATGGTGG+TGG | - | chr1.2:71208110-71208129 | MS.gene60721:CDS | 55.0% | |
| ! | AGGTTGCTTTGCAGGAGGCA+CGG | - | chr1.2:71208320-71208339 | MS.gene60721:CDS | 55.0% |
| !! | CAGGATGGACTGAAGACCAC+GGG | - | chr1.2:71208897-71208916 | MS.gene60721:CDS | 55.0% |
| !! | GCAAGACATGGTGGTGGTAG+AGG | - | chr1.2:71208116-71208135 | MS.gene60721:CDS | 55.0% |
| !! | GGAGCTGCTGCACTGATTGT+TGG | - | chr1.2:71208486-71208505 | MS.gene60721:CDS | 55.0% |
| CCACGGGAGAAGGACTTGAG+TGG | - | chr1.2:71208913-71208932 | MS.gene60721:CDS | 60.0% | |
| CCACTCAAGTCCTTCTCCCG+TGG | + | chr1.2:71208916-71208935 | None:intergenic | 60.0% | |
| GGACTGAAGACCACGGGAGA+AGG | - | chr1.2:71208903-71208922 | MS.gene60721:CDS | 60.0% | |
| ! | AGGAGGCACGGTGCTTCGTT+TGG | - | chr1.2:71208332-71208351 | MS.gene60721:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 71207713 | 71209004 | 71207713 | ID=MS.gene60721 |
| chr1.2 | mRNA | 71207713 | 71209004 | 71207713 | ID=MS.gene60721.t1;Parent=MS.gene60721 |
| chr1.2 | exon | 71208827 | 71209004 | 71208827 | ID=MS.gene60721.t1.exon1;Parent=MS.gene60721.t1 |
| chr1.2 | CDS | 71208827 | 71209004 | 71208827 | ID=cds.MS.gene60721.t1;Parent=MS.gene60721.t1 |
| chr1.2 | exon | 71207713 | 71208704 | 71207713 | ID=MS.gene60721.t1.exon2;Parent=MS.gene60721.t1 |
| chr1.2 | CDS | 71207713 | 71208704 | 71207713 | ID=cds.MS.gene60721.t1;Parent=MS.gene60721.t1 |
| Gene Sequence |
| Protein sequence |
